Prupe.7G137800_v2.0.a1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0012519
Physical Location & Seq
Reverse (-)
15431308 .. 15434051
2744 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G137800.1

Sequence Viewer

Length: 750 bp
ATGGCCAATCGGTGGCGCTCAGCCGGCGGCAACCTCCGTGCGGTTCTGCAAAACCCTAGCTCGCGACAACAAGCTCCATTTCAATCCTTTTCGGCTCTGTATCACACGATCCAAGCCATCCCGAGGGAGTGCACAGGGAACAGAGTCTCCGTCAAAGACCGAGCTCAGGGTCGAATTCCAGCCGTCGTGTTCTCGCAGGAGCTAAACCCTAGCACCACCACCGTTCGATCGGTCTCCAAAAAGCACTTGTTGACCGCCGAGAGGAAGCAGATTCAGGCCATTCTCAAGTCAGTTGAGCTCCCGTTTTTCTGCTCCACTAGGTTCCCGCTCCAGATCCGAGCTGGTTCCGGGTCATCGGATCTTCTTGAATCTGGAAACGTCCTACCCATCAAGATTCATAGGGACGAAGAGAGTGGGAAGATACTGAATTTGGTGTTTGTTTGGGCGGATGAGGGCTCGCAGCTGAAAGTGGATGTCCCTGTTGTTTTCAAAGGTGAAGATGTTTGCCCTGGTCTCAAAAAAGGTGGCCATCTGAACAAAATTAGAACTAGTCTAAAGTACCTTTGCCCAGCTGAACACATTCCTCCCAAAATTGAGGTGGATGTCAGCAAGCTAGATATAGGTGATAGGGTGTTCATCCCTGATGTTGAGGTTCATCCATCCATGAAGCTTCTAAGTAAGAATGAAACCATGCCTATATGTAAGATAGTGGCAACAAAGTTGGAGAACCCAGAATCTGCAGGAGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L25p Ribosomal_TL5_C

Protein Analysis

250

Amino Acids

27.47

Weight (kDa)

9.42

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0012519)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G66860
fragaria_vesca FvH4_1g15520
malus_domestica MD02G1167000.v1.1 MD15G1279600.v1.1
prunus_persica Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1 Prupe.7G137800_v2.0.a1
pyrus_communis pycom02g13450
rosa_chinensis RchiOBHm_Chr2g0104801
rosa_laevigata RLG00000017358
rosa_multiflora Rmu_ssc0000030.1_g000001
rosa_rugosa Rorug02G0123000
rosa_samantha Rh2AG172900 Rh2BG180500 Rh2CG178100 Rh2DG179100
rosa_wichuraiana Rw0G008670 Rw2G013510 Rw2G013590

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 12
AccBSI CCGCTC 1 cut(s) 328
AccII CGCG 1 cut(s) 64
AciI CCGC 5 cut(s) 27, 41, 255, 326, 446
AclWI GGATC 3 cut(s) 103, 328, 366
AcoI YGGCCR 2 cut(s) 3, 526
AcsI RAATTY 2 cut(s) 174, 427
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 5 cut(s) 12, 40, 123, 166, 261
AgsI TTSAA 3 cut(s) 83, 368, 490
AhlI ACTAGT 1 cut(s) 548
AjnI CCWGG 1 cut(s) 508
AloI GAACNNNNNNTCC 2 cut(s) 131, 163
Alw21I GWGCWC 3 cut(s) 134, 166, 300
Alw26I GTCTC 3 cut(s) 151, 238, 518
Alw44I GTGCAC 1 cut(s) 130
AlwI GGATC 3 cut(s) 103, 328, 366
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 121
AoxI GGCC 3 cut(s) 3, 276, 526
ApaLI GTGCAC 1 cut(s) 130
ApeKI GCWGC 1 cut(s) 460
ApoI RAATTY 2 cut(s) 174, 427
Asp700I GAANNNNTTC 1 cut(s) 579
AspLEI GCGC 1 cut(s) 18
AsuC2I CCSGG 1 cut(s) 349
AsuHPI GGTGA 2 cut(s) 506, 635
AvaI CYCGRG 1 cut(s) 121
BaeGI GKGCMC 1 cut(s) 134
BalI TGGCCA 2 cut(s) 5, 528
BanII GRGCYC 3 cut(s) 166, 300, 458
Bbv12I GWGCWC 3 cut(s) 134, 166, 300
BbvI GCAGC 1 cut(s) 472
BccI CCATC 4 cut(s) 125, 395, 537, 667
BceAI ACGGC 1 cut(s) 167
BciT130I CCWGG 1 cut(s) 510
BcnI CCSGG 1 cut(s) 349
BcoDI GTCTC 3 cut(s) 151, 238, 518
BcuI ACTAGT 1 cut(s) 548
BfaI CTAG 5 cut(s) 57, 210, 318, 549, 614
BfmI CTRYAG 1 cut(s) 738
BfoI RGCGCY 1 cut(s) 19
BisI GCNGC 2 cut(s) 28, 461
BlpI GCTNAGC 1 cut(s) 19
BlsI GCNGC 2 cut(s) 29, 462
Bme1390I CCNGG 2 cut(s) 349, 510
BmeT110I CYCGRG 1 cut(s) 121
BmiI GGNNCC 2 cut(s) 323, 346
BmrFI CCNGG 2 cut(s) 349, 510
BpmI CTGGAG 1 cut(s) 314
Bpu10I CCTNAGC 1 cut(s) 165
Bpu1102I GCTNAGC 1 cut(s) 19
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 349
BsaI GGTCTC 2 cut(s) 238, 518
BsaJI CCNNGG 2 cut(s) 122, 508
BsaXI ACNNNNNCTCC 2 cut(s) 131, 161
Bsc4I CCNNNNNNNGG 5 cut(s) 12, 40, 123, 166, 261
Bse118I RCCGGY 1 cut(s) 23
BseBI CCWGG 1 cut(s) 510
BseDI CCNNGG 2 cut(s) 122, 508
BseGI GGATG 7 cut(s) 117, 454, 478, 607, 636, 655, 659
BseLI CCNNNNNNNGG 5 cut(s) 12, 40, 123, 166, 261
BseMII CTCAG 2 cut(s) 33, 179
BseSI GKGCMC 1 cut(s) 134
BseXI GCAGC 1 cut(s) 472
BseYI CCCAGC 1 cut(s) 568
Bsh1236I CGCG 1 cut(s) 64
Bsh1285I CGRYCG 1 cut(s) 230
BshFI GGCC 3 cut(s) 5, 278, 528
BsiEI CGRYCG 1 cut(s) 230
BsiHKAI GWGCWC 3 cut(s) 134, 166, 300
BsiHKCI CYCGRG 1 cut(s) 121
BsiSI CCGG 2 cut(s) 24, 348
BslFI GGGAC 2 cut(s) 416, 461
BslI CCNNNNNNNGG 5 cut(s) 12, 40, 123, 166, 261
BsmAI GTCTC 3 cut(s) 151, 238, 518
BsmFI GGGAC 2 cut(s) 416, 461
BsnI GGCC 3 cut(s) 5, 278, 528
Bso31I GGTCTC 2 cut(s) 238, 518
BsoBI CYCGRG 1 cut(s) 121
Bsp1286I GDGCHC 4 cut(s) 134, 166, 300, 458
Bsp143I GATC 4 cut(s) 108, 227, 333, 358
Bsp1720I GCTNAGC 1 cut(s) 19
Bsp68I TCGCGA 1 cut(s) 64
BspACI CCGC 5 cut(s) 27, 41, 255, 326, 446
BspANI GGCC 3 cut(s) 5, 278, 528
BspCNI CTCAG 2 cut(s) 32, 178
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 323, 346
BspMAI CTGCAG 1 cut(s) 742
BspPI GGATC 3 cut(s) 103, 328, 366
BspTNI GGTCTC 2 cut(s) 238, 518
BsrBI CCGCTC 1 cut(s) 328
BsrFI RCCGGY 1 cut(s) 23
BssAI RCCGGY 1 cut(s) 23
BssECI CCNNGG 2 cut(s) 122, 508
BssMI GATC 4 cut(s) 108, 227, 333, 358
Bst2UI CCWGG 1 cut(s) 510
Bst4CI ACNGT 1 cut(s) 223
Bst6I CTCTTC 1 cut(s) 402
BstC8I GCNNGC 4 cut(s) 25, 62, 458, 611
BstDEI CTNAG 3 cut(s) 19, 165, 674
BstF5I GGATG 7 cut(s) 117, 454, 478, 607, 636, 655, 659
BstFNI CGCG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 19
BstHHI GCGC 1 cut(s) 18
BstKTI GATC 4 cut(s) 111, 230, 336, 361
BstMAI GTCTC 3 cut(s) 151, 238, 518
BstMBI GATC 4 cut(s) 108, 227, 333, 358
BstMCI CGRYCG 1 cut(s) 230
BstMWI GCNNNNNNNGC 1 cut(s) 24
BstNI CCWGG 1 cut(s) 510
BstSCI CCNGG 2 cut(s) 347, 508
BstSFI CTRYAG 1 cut(s) 738
BstSLI GKGCMC 1 cut(s) 134
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 1 cut(s) 472
BstX2I RGATCY 2 cut(s) 333, 358
BstYI RGATCY 2 cut(s) 333, 358
BsuRI GGCC 3 cut(s) 5, 278, 528
BtsCI GGATG 7 cut(s) 117, 454, 478, 607, 636, 655, 659
BtuMI TCGCGA 1 cut(s) 64
Cac8I GCNNGC 4 cut(s) 25, 62, 458, 611
CaiI CAGNNNCTG 1 cut(s) 737
CfoI GCGC 1 cut(s) 18
Cfr10I RCCGGY 1 cut(s) 23
Csp6I GTAC 1 cut(s) 559
CspCI CAANNNNNGTGG 2 cut(s) 505, 540
CviAII CATG 2 cut(s) 664, 691
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 3 cut(s) 19, 165, 674
DpnI GATC 4 cut(s) 110, 229, 335, 360
DpnII GATC 4 cut(s) 108, 227, 333, 358
EaeI YGGCCR 2 cut(s) 3, 526
Eam1104I CTCTTC 1 cut(s) 402
EarI CTCTTC 1 cut(s) 402
EciI GGCGGA 1 cut(s) 461
Ecl136II GAGCTC 2 cut(s) 164, 298
Eco24I GRGCYC 3 cut(s) 166, 300, 458
Eco31I GGTCTC 2 cut(s) 238, 518
Eco53kI GAGCTC 2 cut(s) 164, 298
Eco88I CYCGRG 1 cut(s) 121
EcoICRI GAGCTC 2 cut(s) 164, 298
EcoRI GAATTC 1 cut(s) 174
EcoRII CCWGG 1 cut(s) 508
EcoT38I GRGCYC 3 cut(s) 166, 300, 458
FaeI CATG 2 cut(s) 667, 694
FaiI YATR 6 cut(s) 399, 620, 665, 692, 698, 700
FaqI GGGAC 2 cut(s) 416, 461
FatI CATG 2 cut(s) 663, 690
FauI CCCGC 1 cut(s) 333
Fnu4HI GCNGC 2 cut(s) 28, 461
FokI GGATG 7 cut(s) 104, 461, 485, 614, 623, 642, 646
FriOI GRGCYC 3 cut(s) 166, 300, 458
Fsp4HI GCNGC 2 cut(s) 28, 461
FspBI CTAG 5 cut(s) 57, 210, 318, 549, 614
GlaI GCGC 1 cut(s) 17
GluI GCNGC 2 cut(s) 28, 461
GsaI CCCAGC 1 cut(s) 572
GsuI CTGGAG 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 19
HaeIII GGCC 3 cut(s) 5, 278, 528
HapII CCGG 2 cut(s) 24, 348
HhaI GCGC 1 cut(s) 18
Hin1II CATG 2 cut(s) 667, 694
Hin6I GCGC 1 cut(s) 16
HinP1I GCGC 1 cut(s) 16
HincII GTYRAC 1 cut(s) 252
HindII GTYRAC 1 cut(s) 252
HindIII AAGCTT 1 cut(s) 668
HinfI GANTC 5 cut(s) 144, 271, 368, 394, 734
HpaII CCGG 2 cut(s) 24, 348
HphI GGTGA 2 cut(s) 506, 635
Hpy166II GTNNAC 2 cut(s) 132, 252
Hpy188I TCNGA 3 cut(s) 338, 358, 534
Hpy188III TCNNGA 6 cut(s) 63, 121, 331, 365, 372, 391
Hpy8I GTNNAC 2 cut(s) 132, 252
Hpy99I CGWCG 1 cut(s) 188
HpyCH4III ACNGT 1 cut(s) 223
HpyCH4IV ACGT 1 cut(s) 378
HpyCH4V TGCA 3 cut(s) 49, 132, 740
HpyF10VI GCNNNNNNNGC 1 cut(s) 24
HpyF3I CTNAG 3 cut(s) 19, 165, 674
HpySE526I ACGT 1 cut(s) 378
Hsp92II CATG 2 cut(s) 667, 694
HspAI GCGC 1 cut(s) 16
KroI GCCGGC 1 cut(s) 23
KroNI GCCGGC 1 cut(s) 25
Kzo9I GATC 4 cut(s) 108, 227, 333, 358
LmnI GCTCC 5 cut(s) 79, 199, 303, 317, 333
Lsp1109I GCAGC 1 cut(s) 472
MaeI CTAG 5 cut(s) 57, 210, 318, 549, 614
MaeII ACGT 1 cut(s) 378
MalI GATC 4 cut(s) 110, 229, 335, 360
MbiI CCGCTC 1 cut(s) 328
MboI GATC 4 cut(s) 108, 227, 333, 358
MboII GAAGA 4 cut(s) 353, 419, 430, 509
MflI RGATCY 2 cut(s) 333, 358
MhlI GDGCHC 4 cut(s) 134, 166, 300, 458
MlsI TGGCCA 2 cut(s) 5, 528
MluCI AATT 4 cut(s) 174, 427, 540, 591
MluNI TGGCCA 2 cut(s) 5, 528
MlyI GAGTC 1 cut(s) 153
MmeI TCCRAC 1 cut(s) 702
MnlI CCTC 7 cut(s) 44, 117, 255, 445, 589, 594, 643
Mox20I TGGCCA 2 cut(s) 5, 528
MroNI GCCGGC 1 cut(s) 23
MroXI GAANNNNTTC 1 cut(s) 579
MscI TGGCCA 2 cut(s) 5, 528
Msp20I TGGCCA 2 cut(s) 5, 528
MspA1I CMGCKG 2 cut(s) 463, 572
MspI CCGG 2 cut(s) 24, 348
MspR9I CCNGG 2 cut(s) 349, 510
MvaI CCWGG 1 cut(s) 510
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 1 cut(s) 24
NaeI GCCGGC 1 cut(s) 25
NciI CCSGG 1 cut(s) 349
NdeII GATC 4 cut(s) 108, 227, 333, 358
NgoMIV GCCGGC 1 cut(s) 23
NlaIII CATG 2 cut(s) 667, 694
NlaIV GGNNCC 2 cut(s) 323, 346
NmeAIII GCCGAG 1 cut(s) 283
NruI TCGCGA 1 cut(s) 64
PdiI GCCGGC 1 cut(s) 25
PdmI GAANNNNTTC 1 cut(s) 579
PfeI GAWTC 4 cut(s) 271, 368, 394, 734
PflMI CCANNNNNTGG 1 cut(s) 12
PkrI GCNGC 2 cut(s) 29, 462
Ple19I CGATCG 1 cut(s) 230
PleI GAGTC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 152
Psp124BI GAGCTC 2 cut(s) 166, 300
Psp6I CCWGG 1 cut(s) 508
PspFI CCCAGC 1 cut(s) 568
PspGI CCWGG 1 cut(s) 508
PspN4I GGNNCC 2 cut(s) 323, 346
PstI CTGCAG 1 cut(s) 742
PstNI CAGNNNCTG 1 cut(s) 737
PsuI RGATCY 2 cut(s) 333, 358
PvuI CGATCG 1 cut(s) 230
PvuII CAGCTG 2 cut(s) 463, 572
RruI TCGCGA 1 cut(s) 64
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
SacI GAGCTC 2 cut(s) 166, 300
SatI GCNGC 2 cut(s) 28, 461
Sau3AI GATC 4 cut(s) 108, 227, 333, 358
SchI GAGTC 1 cut(s) 153
ScrFI CCNGG 2 cut(s) 349, 510
SduI GDGCHC 4 cut(s) 134, 166, 300, 458
SfcI CTRYAG 1 cut(s) 738
SmlI CTYRAG 1 cut(s) 284
SmoI CTYRAG 1 cut(s) 284
SpeI ACTAGT 1 cut(s) 548
Sse9I AATT 4 cut(s) 174, 427, 540, 591
SsiI CCGC 5 cut(s) 27, 41, 255, 326, 446
SspMI CTAG 5 cut(s) 57, 210, 318, 549, 614
SstI GAGCTC 2 cut(s) 166, 300
StyD4I CCNGG 2 cut(s) 347, 508
TaaI ACNGT 1 cut(s) 223
TaiI ACGT 1 cut(s) 381
TaqI TCGA 2 cut(s) 172, 226
TaqII GACCGA 2 cut(s) 174, 220
TasI AATT 4 cut(s) 174, 427, 540, 591
TauI GCSGC 1 cut(s) 30
TfiI GAWTC 4 cut(s) 271, 368, 394, 734
TseI GCWGC 1 cut(s) 460
TspDTI ATGAA 5 cut(s) 386, 625, 644, 680, 699
TspGWI ACGGA 2 cut(s) 26, 139
Van91I CCANNNNNTGG 1 cut(s) 12
VneI GTGCAC 1 cut(s) 130
XapI RAATTY 2 cut(s) 174, 427
XcmI CCANNNNNNNNNTGG 2 cut(s) 338, 595
XmnI GAANNNNTTC 1 cut(s) 579
XspI CTAG 5 cut(s) 57, 210, 318, 549, 614
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.