AT5G67220

tRNA-dihydrouridine(16 17) synthase NAD(P)( ) -like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0015403
Physical Location & Seq
Reverse (-)
26820121 .. 26821964
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G67220.1

Sequence Viewer

Length: 1272 bp
ATGAAACTAAATCTCTCGAATCTCAGATTTCTACGTACCCGAAAATCGTTAATCTCCCAGACGCGAGCAATGACTCAAAATCCGGATCCAAAACCTGATCCATCGCAGGTTCTAGACGATATCCTCTGTTCGGAGCAGCGTGATGGGCAGATTGAGGAAACAGTCGACACAGCGCCGGCGAGCTTGGGCTCTCCAAGTCGGGTCTTAAGCATTGATACTAGAGTAGAGAGAGCTTGGGCACACTGGAAAAAACTGGGTAGACCCAAGTATATCGTTGCTCCAATGGTTGATAACTCTGAGCTTCCGTTTAGATTGCTCTGCCAGAAATACGGAGCTCAGGCTGCTTATACTCCGATGTTGCATTCTAGGATCTTCACCGAGACTGAGAAGTATAGAAATCAGGAGTTCACCACCTGTAAGGAGGACAGGCCATTGTTTGTGCAGTTCTGTGCTAATGATCCTGATACGTTATTGGAAGCTGCAAAGAGAGTCGAACCTTACTGCGACTATGTTGATATCAATTTAGGGTGTCCTCAGCGTATAGCGAGGCGAGGAAATTATGGTGCATTCTTGATGGATAATCTTCCTTTGGTGAAATCACTTGTTGAAAAGTTAGCTCAGAACCTCAATGTTCCTGTCTCCTGTAAAATCCGGATCTTCCCGAACCTGGAAGATACACTCAAGTACGCCAAGATGCTAGAAGATGCTGGTTGCTCGCTCCTAGCTGTTCACGGGCGAACAAGAGATGAGAAAGACGGGAAGAAATTTAGAGCTGATTGGAGCGCAATCAAGGAAGTGAAAAACGCTATGAGAATCCCTGTCTTAGCGAATGGGAATGTAAGATGCATCGAAGATGTCGATAACTGCATCAAAGAGACGGGTGTTGAAGGTGTTCTCTCTGCGGAGACGCTTCTTGAAAACCCGGCGGCCTTTGCTGGGTTTAGAACAGCTGAATGGGCAAAAGATAACGAAGAAGAGGGATTCGTCGATGGAGGGTTAGACCAGGGAGATTTAGTTGTTGAGTATTTAAAGCTGTGTGAGAAGCATCCGGTTCCATGGAGGATGATTCGATCTCACGTTCATAAGATGTTGGGAGAATGGTTTAGAATTCATCCACAAGTTAGAGAGCAACTTAATGCTCAAAACATATTGACGTTTGAGTTTCTATACGGACTTGTGGATCAGCTAAGAGAGCTTGGAGGAAGAGTTCCACTCTACAAGAAAAAGAAGATAGATACTCTGACTCCACAAGACTCTCCACAAAGGGTTTAG

Protein Analysis

423

Amino Acids

48.24

Weight (kDa)

7.51

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 92 - 380 9.4e-72 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67220
fragaria_vesca FvH4_1g16470
malus_domestica MD02G1177400.v1.1 MD15G1287200.v1.1
prunus_persica Prupe.6G230700_v2.0.a1
pyrus_communis pycom15g25070
rosa_chinensis RchiOBHm_Chr2g0106351
rosa_laevigata RLG00000017491
rosa_multiflora Rmu_sc0010122.1_g000004
rosa_roxburghii Rroxscaffold_2G00137260
rosa_rugosa Rorug02G0134900
rosa_samantha Rh2AG186000 Rh2BG196200 Rh2CG191000 Rh2DG192100
rosa_wichuraiana Rw2G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 97
AccI GTMKAC 2 cut(s) 165, 259
AccII CGCG 1 cut(s) 64
AccIII TCCGGA 2 cut(s) 82, 651
AciI CCGC 2 cut(s) 902, 926
AclWI GGATC 7 cut(s) 80, 92, 93, 377, 452, 662, 1188
AcsI RAATTY 2 cut(s) 764, 1107
AfaI GTAC 2 cut(s) 37, 686
AfiI CCNNNNNNNGG 3 cut(s) 130, 667, 936
AflII CTTAAG 1 cut(s) 205
AgsI TTSAA 3 cut(s) 608, 887, 917
AjnI CCWGG 2 cut(s) 666, 1002
AloI GAACNNNNNNTCC 2 cut(s) 1191, 1223
Alw21I GWGCWC 1 cut(s) 337
Alw26I GTCTC 4 cut(s) 374, 643, 869, 899
AlwI GGATC 7 cut(s) 80, 92, 93, 377, 452, 662, 1188
Aor13HI TCCGGA 2 cut(s) 82, 651
AoxI GGCC 2 cut(s) 428, 927
ApeKI GCWGC 3 cut(s) 136, 341, 479
ApoI RAATTY 2 cut(s) 764, 1107
Asp700I GAANNNNTTC 1 cut(s) 891
AspLEI GCGC 2 cut(s) 175, 785
AsuC2I CCSGG 1 cut(s) 923
AsuHPI GGTGA 3 cut(s) 367, 400, 604
BaeGI GKGCMC 1 cut(s) 241
BaeI ACNNNNGTAYC 2 cut(s) 207, 240
BamHI GGATCC 1 cut(s) 85
BanII GRGCYC 2 cut(s) 191, 337
Bbv12I GWGCWC 1 cut(s) 337
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 3 cut(s) 148, 328, 466
BccI CCATC 4 cut(s) 109, 137, 568, 983
BciT130I CCWGG 2 cut(s) 668, 1004
BcnI CCSGG 1 cut(s) 923
BcoDI GTCTC 4 cut(s) 374, 643, 869, 899
BfaI CTAG 5 cut(s) 113, 219, 366, 698, 722
BfoI RGCGCY 1 cut(s) 176
BfrI CTTAAG 1 cut(s) 205
BfuAI ACCTGC 1 cut(s) 97
BisI GCNGC 4 cut(s) 137, 342, 480, 927
BlsI GCNGC 4 cut(s) 138, 343, 481, 928
Bme1390I CCNGG 3 cut(s) 668, 923, 1004
BmiI GGNNCC 2 cut(s) 87, 1053
BmrFI CCNGG 3 cut(s) 668, 923, 1004
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 6 cut(s) 684, 694, 833, 855, 876, 1054
BmuI ACTGGG 1 cut(s) 263
BplI GAGNNNNNCTC 2 cut(s) 1197, 1229
Bpu10I CCTNAGC 2 cut(s) 336, 534
BpuEI CTTGAG 1 cut(s) 665
BpuMI CCSGG 1 cut(s) 923
BsaAI YACGTR 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 1003, 1055
BsaWI WCCGGW 3 cut(s) 82, 651, 1048
BsaXI ACNNNNNCTCC 6 cut(s) 999, 1029, 1191, 1221, 1228, 1258
Bsc4I CCNNNNNNNGG 3 cut(s) 130, 667, 936
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 2 cut(s) 248, 258
Bse3DI GCAATG 1 cut(s) 75
BseAI TCCGGA 2 cut(s) 82, 651
BseBI CCWGG 2 cut(s) 668, 1004
BseDI CCNNGG 2 cut(s) 1003, 1055
BseGI GGATG 3 cut(s) 1045, 1068, 1111
BseLI CCNNNNNNNGG 3 cut(s) 130, 667, 936
BseMI GCAATG 1 cut(s) 75
BseMII CTCAG 6 cut(s) 37, 288, 350, 375, 548, 632
BseNI ACTGG 2 cut(s) 248, 258
BseSI GKGCMC 1 cut(s) 241
BseXI GCAGC 3 cut(s) 148, 328, 466
BseYI CCCAGC 1 cut(s) 935
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 64
BshFI GGCC 2 cut(s) 430, 929
BsiHKAI GWGCWC 1 cut(s) 337
BsiSI CCGG 5 cut(s) 83, 176, 652, 923, 1049
BslI CCNNNNNNNGG 3 cut(s) 130, 667, 936
BsmAI GTCTC 4 cut(s) 374, 643, 869, 899
BsmBI CGTCTC 2 cut(s) 869, 899
BsmI GAATGC 2 cut(s) 361, 566
BsnI GGCC 2 cut(s) 430, 929
Bsp1286I GDGCHC 3 cut(s) 191, 241, 337
Bsp13I TCCGGA 2 cut(s) 82, 651
Bsp143I GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
Bsp19I CCATGG 1 cut(s) 1055
BspACI CCGC 2 cut(s) 902, 926
BspANI GGCC 2 cut(s) 430, 929
BspCNI CTCAG 6 cut(s) 36, 289, 349, 376, 547, 631
BspEI TCCGGA 2 cut(s) 82, 651
BspFNI CGCG 1 cut(s) 64
BspLI GGNNCC 2 cut(s) 87, 1053
BspMI ACCTGC 1 cut(s) 97
BspPI GGATC 7 cut(s) 80, 92, 93, 377, 452, 662, 1188
BspTI CTTAAG 1 cut(s) 205
BsrDI GCAATG 1 cut(s) 75
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 2 cut(s) 248, 258
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 2 cut(s) 1003, 1055
BssMI GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
BssT1I CCWWGG 1 cut(s) 1055
Bst2UI CCWGG 2 cut(s) 668, 1004
Bst4CI ACNGT 1 cut(s) 163
Bst6I CTCTTC 2 cut(s) 969, 1198
BstAFI CTTAAG 1 cut(s) 205
BstBAI YACGTR 1 cut(s) 35
BstC8I GCNNGC 4 cut(s) 66, 177, 181, 716
BstDEI CTNAG 8 cut(s) 23, 297, 336, 384, 534, 618, 823, 1187
BstDSI CCRYGG 1 cut(s) 1055
BstF5I GGATG 3 cut(s) 1045, 1068, 1111
BstFNI CGCG 1 cut(s) 64
BstH2I RGCGCY 1 cut(s) 176
BstHHI GCGC 2 cut(s) 175, 785
BstKTI GATC 7 cut(s) 88, 100, 372, 460, 657, 1073, 1183
BstMAI GTCTC 4 cut(s) 374, 643, 869, 899
BstMBI GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
BstMWI GCNNNNNNNGC 5 cut(s) 145, 341, 932, 956, 1192
BstNI CCWGG 2 cut(s) 668, 1004
BstSCI CCNGG 3 cut(s) 666, 921, 1002
BstSLI GKGCMC 1 cut(s) 241
BstSNI TACGTA 1 cut(s) 35
BstUI CGCG 1 cut(s) 64
BstV1I GCAGC 3 cut(s) 148, 328, 466
BstX2I RGATCY 3 cut(s) 85, 369, 654
BstYI RGATCY 3 cut(s) 85, 369, 654
BsuRI GGCC 2 cut(s) 430, 929
BtgI CCRYGG 1 cut(s) 1055
BtgZI GCGATG 1 cut(s) 87
BtsCI GGATG 3 cut(s) 1045, 1068, 1111
BtsIMutI CAGTG 1 cut(s) 241
BveI ACCTGC 1 cut(s) 97
Cac8I GCNNGC 4 cut(s) 66, 177, 181, 716
CfoI GCGC 2 cut(s) 175, 785
Cfr10I RCCGGY 1 cut(s) 175
CseI GACGC 2 cut(s) 70, 916
Csp6I GTAC 2 cut(s) 36, 685
CviAII CATG 1 cut(s) 1056
CviQI GTAC 2 cut(s) 36, 685
DdeI CTNAG 8 cut(s) 23, 297, 336, 384, 534, 618, 823, 1187
DpnI GATC 7 cut(s) 87, 99, 371, 459, 656, 1072, 1182
DpnII GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
DraI TTTAAA 1 cut(s) 1029
Eam1104I CTCTTC 2 cut(s) 969, 1198
EarI CTCTTC 2 cut(s) 969, 1198
Ecl136II GAGCTC 1 cut(s) 335
Eco105I TACGTA 1 cut(s) 35
Eco130I CCWWGG 1 cut(s) 1055
Eco24I GRGCYC 2 cut(s) 191, 337
Eco32I GATATC 2 cut(s) 121, 517
Eco53kI GAGCTC 1 cut(s) 335
EcoICRI GAGCTC 1 cut(s) 335
EcoRI GAATTC 1 cut(s) 1107
EcoRII CCWGG 2 cut(s) 666, 1002
EcoRV GATATC 2 cut(s) 121, 517
EcoT14I CCWWGG 1 cut(s) 1055
EcoT22I ATGCAT 1 cut(s) 848
EcoT38I GRGCYC 2 cut(s) 191, 337
ErhI CCWWGG 1 cut(s) 1055
Esp3I CGTCTC 2 cut(s) 869, 899
FaeI CATG 1 cut(s) 1059
FatI CATG 1 cut(s) 1055
FblI GTMKAC 2 cut(s) 165, 259
Fnu4HI GCNGC 4 cut(s) 137, 342, 480, 927
FokI GGATG 3 cut(s) 1032, 1075, 1098
FriOI GRGCYC 2 cut(s) 191, 337
Fsp4HI GCNGC 4 cut(s) 137, 342, 480, 927
FspBI CTAG 5 cut(s) 113, 219, 366, 698, 722
GlaI GCGC 2 cut(s) 174, 784
GluI GCNGC 4 cut(s) 137, 342, 480, 927
GsaI CCCAGC 1 cut(s) 939
HaeII RGCGCY 1 cut(s) 176
HaeIII GGCC 2 cut(s) 430, 929
HapII CCGG 5 cut(s) 83, 176, 652, 923, 1049
HgaI GACGC 2 cut(s) 70, 916
HhaI GCGC 2 cut(s) 175, 785
Hin1II CATG 1 cut(s) 1059
Hin6I GCGC 2 cut(s) 173, 783
HinP1I GCGC 2 cut(s) 173, 783
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HinfI GANTC 8 cut(s) 19, 73, 489, 813, 981, 1066, 1243, 1253
HpaII CCGG 5 cut(s) 83, 176, 652, 923, 1049
HphI GGTGA 3 cut(s) 367, 400, 604
Hpy166II GTNNAC 4 cut(s) 166, 260, 408, 730
Hpy188I TCNGA 6 cut(s) 26, 133, 298, 354, 621, 1242
Hpy188III TCNNGA 9 cut(s) 16, 83, 113, 401, 461, 571, 652, 661, 914
Hpy8I GTNNAC 4 cut(s) 166, 260, 408, 730
Hpy99I CGWCG 1 cut(s) 989
HpyAV CCTTC 1 cut(s) 881
HpyCH4III ACNGT 1 cut(s) 163
HpyCH4IV ACGT 4 cut(s) 34, 467, 1077, 1154
HpyCH4V TGCA 6 cut(s) 361, 442, 482, 566, 846, 867
HpyF10VI GCNNNNNNNGC 5 cut(s) 145, 341, 932, 956, 1192
HpyF3I CTNAG 8 cut(s) 23, 297, 336, 384, 534, 618, 823, 1187
HpySE526I ACGT 4 cut(s) 34, 467, 1077, 1154
Hsp92II CATG 1 cut(s) 1059
HspAI GCGC 2 cut(s) 173, 783
Kpn2I TCCGGA 2 cut(s) 82, 651
KroI GCCGGC 1 cut(s) 175
KroNI GCCGGC 1 cut(s) 177
Kzo9I GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
LmnI GCTCC 5 cut(s) 133, 283, 332, 723, 780
Lsp1109I GCAGC 3 cut(s) 148, 328, 466
LweI GCATC 6 cut(s) 684, 694, 833, 855, 876, 1054
MaeI CTAG 5 cut(s) 113, 219, 366, 698, 722
MaeII ACGT 4 cut(s) 34, 467, 1077, 1154
MalI GATC 7 cut(s) 87, 99, 371, 459, 656, 1072, 1182
MboI GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
MflI RGATCY 3 cut(s) 85, 369, 654
MhlI GDGCHC 3 cut(s) 191, 241, 337
MluCI AATT 4 cut(s) 520, 556, 764, 1107
MlyI GAGTC 4 cut(s) 67, 498, 1237, 1247
Mph1103I ATGCAT 1 cut(s) 848
MreI CGCCGGCG 1 cut(s) 175
MroI TCCGGA 2 cut(s) 82, 651
MroNI GCCGGC 1 cut(s) 175
MroXI GAANNNNTTC 1 cut(s) 891
MseI TTAA 4 cut(s) 50, 206, 1028, 1134
MspA1I CMGCKG 1 cut(s) 950
MspCI CTTAAG 1 cut(s) 205
MspI CCGG 5 cut(s) 83, 176, 652, 923, 1049
MspR9I CCNGG 3 cut(s) 668, 923, 1004
Mva1269I GAATGC 2 cut(s) 361, 566
MvaI CCWGG 2 cut(s) 668, 1004
MvnI CGCG 1 cut(s) 64
MwoI GCNNNNNNNGC 5 cut(s) 145, 341, 932, 956, 1192
NaeI GCCGGC 1 cut(s) 177
NciI CCSGG 1 cut(s) 923
NcoI CCATGG 1 cut(s) 1055
NdeII GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
NgoMIV GCCGGC 1 cut(s) 175
NlaIII CATG 1 cut(s) 1059
NlaIV GGNNCC 2 cut(s) 87, 1053
NsiI ATGCAT 1 cut(s) 848
PcsI WCGNNNNNNNCGW 1 cut(s) 855
PctI GAATGC 2 cut(s) 361, 566
PdiI GCCGGC 1 cut(s) 177
PdmI GAANNNNTTC 1 cut(s) 891
PfeI GAWTC 4 cut(s) 19, 813, 981, 1066
PkrI GCNGC 4 cut(s) 138, 343, 481, 928
PleI GAGTC 4 cut(s) 67, 497, 1237, 1247
PpsI GAGTC 4 cut(s) 67, 497, 1237, 1247
Ppu21I YACGTR 1 cut(s) 35
Psp124BI GAGCTC 1 cut(s) 337
Psp6I CCWGG 2 cut(s) 666, 1002
PspFI CCCAGC 1 cut(s) 935
PspGI CCWGG 2 cut(s) 666, 1002
PspN4I GGNNCC 2 cut(s) 87, 1053
PsuI RGATCY 3 cut(s) 85, 369, 654
PvuII CAGCTG 1 cut(s) 950
RsaI GTAC 2 cut(s) 37, 686
RsaNI GTAC 2 cut(s) 36, 685
SacI GAGCTC 1 cut(s) 337
SalI GTCGAC 1 cut(s) 164
SaqAI TTAA 4 cut(s) 50, 206, 1028, 1134
SatI GCNGC 4 cut(s) 137, 342, 480, 927
Sau3AI GATC 7 cut(s) 85, 97, 369, 457, 654, 1070, 1180
SchI GAGTC 4 cut(s) 67, 498, 1237, 1247
ScrFI CCNGG 3 cut(s) 668, 923, 1004
SduI GDGCHC 3 cut(s) 191, 241, 337
SfaNI GCATC 6 cut(s) 684, 694, 833, 855, 876, 1054
SgrAI CRCCGGYG 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 205, 680
SmoI CTYRAG 2 cut(s) 205, 680
SnaBI TACGTA 1 cut(s) 35
Sse9I AATT 4 cut(s) 520, 556, 764, 1107
SsiI CCGC 2 cut(s) 902, 926
SspMI CTAG 5 cut(s) 113, 219, 366, 698, 722
SstI GAGCTC 1 cut(s) 337
StyD4I CCNGG 3 cut(s) 666, 921, 1002
StyI CCWWGG 1 cut(s) 1055
TaaI ACNGT 1 cut(s) 163
TaiI ACGT 4 cut(s) 37, 470, 1080, 1157
TaqI TCGA 7 cut(s) 17, 165, 492, 849, 858, 987, 1069
TasI AATT 4 cut(s) 520, 556, 764, 1107
TauI GCSGC 1 cut(s) 929
TfiI GAWTC 4 cut(s) 19, 813, 981, 1066
Tru1I TTAA 4 cut(s) 50, 206, 1028, 1134
Tru9I TTAA 4 cut(s) 50, 206, 1028, 1134
TscAI CASTG 1 cut(s) 248
TseI GCWGC 3 cut(s) 136, 341, 479
TspDTI ATGAA 3 cut(s) 17, 1070, 1100
TspGWI ACGGA 3 cut(s) 294, 345, 1185
TspRI CASTG 1 cut(s) 248
Vha464I CTTAAG 1 cut(s) 205
XapI RAATTY 2 cut(s) 764, 1107
XbaI TCTAGA 1 cut(s) 112
XmiI GTMKAC 2 cut(s) 165, 259
XmnI GAANNNNTTC 1 cut(s) 891
XspI CTAG 5 cut(s) 113, 219, 366, 698, 722
Zsp2I ATGCAT 1 cut(s) 848
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.