Rorug02G0134900

tRNA-dihydrouridine(16 17) synthase NAD(P)( ) -like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0015403
Physical Location & Seq
Forward (+)
11665424 .. 11666140
717 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0134900.1

Sequence Viewer

Length: 717 bp
ATGGAACCGCAACACCGGCATCCAACCGAGGATGGAGGAAGTAACAAAGCAGCCGGGAGTAGTGCTAACATGCTTCGCAGGAAGAGCAGTACTAGGTGGATTCCCACTACAGATCATATAAGAATCCTCAAGGAGCTTTTCTACAATAAGGGAGTTAGGTCCCCAACTATAGAGCAGATTCAGAGGATTTGTCTCCAGCTGAAATGGTACGGCAAGATCGAGTTCAAGAACGTCTATTATTGGTTCGTGAACCAAAGGGCTCGGGAGAAGCAGAAGAGGAGGTCCACTTCGGATGTTCATGTGCCCATGCAAAGATCAGGGCTTGTTGGTGATTGGAAACATGAGGATCAGTATATTAACTTTGGATCTTCTGCACCTGCTTCTTTCGCTGGTGTGATGATTGCTTTTAACGGGCAGATGGTGAACTATGGCGGTTATGGATCTATGAACATGGAGAAGAGTGCTAGGGATTGTTCAATCTCAGCTGGAGGGGGAACTAGTTCTACTTTCTTTGAACAAACTTTCATGGAACAAAGAGGAGAAGATCACCAGGAGATTGAAATCATTTCACTATTTCCCATGCATGGTGAGGACATCTTTGGCAACATGAAAACTACTTCCGATGGAGGTGGCGGTCACGGTGGTGGCTCTCACACTTCCCTTGAGCTCAGCCTTTCCTGCGAGGGCAAAGCTGGAAAAAGTTTCCGGAGCCGCTGA

Protein Analysis

238

Amino Acids

26.65

Weight (kDa)

9.1

Isoelectric Point (pI)

60.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homeodomain PF00046 31 - 91 1.8e-08 Homeodomain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0015403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67220
fragaria_vesca FvH4_1g16470
malus_domestica MD02G1177400.v1.1 MD15G1287200.v1.1
prunus_persica Prupe.6G230700_v2.0.a1
pyrus_communis pycom15g25070
rosa_chinensis RchiOBHm_Chr2g0106351
rosa_laevigata RLG00000017491
rosa_multiflora Rmu_sc0010122.1_g000004
rosa_roxburghii Rroxscaffold_2G00137260
rosa_rugosa Rorug02G0134900
rosa_samantha Rh2AG186000 Rh2BG196200 Rh2CG191000 Rh2DG192100
rosa_wichuraiana Rw2G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 385
Acc36I ACCTGC 1 cut(s) 385
AccIII TCCGGA 1 cut(s) 705
AciI CCGC 4 cut(s) 8, 432, 633, 712
AclWI GGATC 3 cut(s) 354, 373, 448
AfaI GTAC 2 cut(s) 91, 209
AfiI CCNNNNNNNGG 1 cut(s) 584
AgsI TTSAA 4 cut(s) 226, 477, 515, 560
AhlI ACTAGT 1 cut(s) 497
AjnI CCWGG 1 cut(s) 549
AleI CACNNNNGTG 1 cut(s) 642
AluBI AGCT 5 cut(s) 136, 199, 485, 667, 692
AluI AGCT 5 cut(s) 136, 199, 485, 667, 692
Alw21I GWGCWC 1 cut(s) 669
Alw26I GTCTC 1 cut(s) 197
AlwI GGATC 3 cut(s) 354, 373, 448
Ama87I CYCGRG 1 cut(s) 261
Aor13HI TCCGGA 1 cut(s) 705
ApeKI GCWGC 1 cut(s) 50
Asp700I GAANNNNTTC 1 cut(s) 499
AspS9I GGNCC 2 cut(s) 159, 282
AsuC2I CCSGG 1 cut(s) 55
AsuHPI GGTGA 4 cut(s) 341, 433, 539, 599
AvaI CYCGRG 1 cut(s) 261
AvaII GGWCC 2 cut(s) 159, 282
BaeGI GKGCMC 1 cut(s) 306
BanII GRGCYC 2 cut(s) 262, 669
Bbv12I GWGCWC 1 cut(s) 669
BbvI GCAGC 1 cut(s) 62
BccI CCATC 3 cut(s) 26, 412, 617
BceAI ACGGC 1 cut(s) 226
BciT130I CCWGG 1 cut(s) 551
BcnI CCSGG 1 cut(s) 55
BcoDI GTCTC 1 cut(s) 197
BcuI ACTAGT 1 cut(s) 497
BfaI CTAG 3 cut(s) 93, 465, 498
BfmI CTRYAG 2 cut(s) 108, 168
BfuAI ACCTGC 1 cut(s) 385
BisI GCNGC 2 cut(s) 51, 712
BlpI GCTNAGC 1 cut(s) 668
BlsI GCNGC 2 cut(s) 52, 713
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 2 cut(s) 55, 551
Bme18I GGWCC 2 cut(s) 159, 282
BmeT110I CYCGRG 1 cut(s) 261
BmgT120I GGNCC 2 cut(s) 159, 282
BmiI GGNNCC 3 cut(s) 6, 161, 710
BmrFI CCNGG 2 cut(s) 55, 551
BmsI GCATC 1 cut(s) 28
BpmI CTGGAG 2 cut(s) 179, 507
Bpu1102I GCTNAGC 1 cut(s) 668
BpuEI CTTGAG 2 cut(s) 113, 683
BpuMI CCSGG 1 cut(s) 55
BsaBI GATNNNNATC 1 cut(s) 560
BsaJI CCNNGG 1 cut(s) 27
BsaWI WCCGGW 1 cut(s) 705
Bsc4I CCNNNNNNNGG 1 cut(s) 584
Bse118I RCCGGY 1 cut(s) 15
Bse8I GATNNNNATC 1 cut(s) 560
BseAI TCCGGA 1 cut(s) 705
BseBI CCWGG 1 cut(s) 551
BseDI CCNNGG 1 cut(s) 27
BseGI GGATG 3 cut(s) 19, 37, 298
BseJI GATNNNNATC 1 cut(s) 560
BseLI CCNNNNNNNGG 1 cut(s) 584
BseMII CTCAG 2 cut(s) 495, 682
BseRI GAGGAG 2 cut(s) 292, 552
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 1 cut(s) 62
BsgI GTGCAG 1 cut(s) 357
BsiHKAI GWGCWC 1 cut(s) 669
BsiHKCI CYCGRG 1 cut(s) 261
BsiSI CCGG 3 cut(s) 16, 54, 706
BslFI GGGAC 1 cut(s) 145
BslI CCNNNNNNNGG 1 cut(s) 584
BsmAI GTCTC 1 cut(s) 197
BsmFI GGGAC 1 cut(s) 145
BsoBI CYCGRG 1 cut(s) 261
Bsp1286I GDGCHC 3 cut(s) 262, 306, 669
Bsp13I TCCGGA 1 cut(s) 705
Bsp143I GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
Bsp1720I GCTNAGC 1 cut(s) 668
BspACI CCGC 4 cut(s) 8, 432, 633, 712
BspCNI CTCAG 2 cut(s) 494, 681
BspEI TCCGGA 1 cut(s) 705
BspLI GGNNCC 3 cut(s) 6, 161, 710
BspMI ACCTGC 1 cut(s) 385
BspPI GGATC 3 cut(s) 354, 373, 448
BspQI GCTCTTC 1 cut(s) 77
BsrFI RCCGGY 1 cut(s) 15
BssAI RCCGGY 1 cut(s) 15
BssECI CCNNGG 1 cut(s) 27
BssMI GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
Bst2UI CCWGG 1 cut(s) 551
Bst4CI ACNGT 1 cut(s) 641
Bst6I CTCTTC 3 cut(s) 77, 269, 452
BstDEI CTNAG 2 cut(s) 481, 668
BstF5I GGATG 3 cut(s) 19, 37, 298
BstKTI GATC 7 cut(s) 115, 219, 317, 349, 368, 443, 547
BstMAI GTCTC 1 cut(s) 197
BstMBI GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
BstMWI GCNNNNNNNGC 4 cut(s) 16, 84, 386, 678
BstNI CCWGG 1 cut(s) 551
BstNSI RCATGY 1 cut(s) 73
BstSCI CCNGG 2 cut(s) 53, 549
BstSFI CTRYAG 2 cut(s) 108, 168
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 1 cut(s) 62
BstX2I RGATCY 2 cut(s) 365, 440
BstYI RGATCY 2 cut(s) 365, 440
BtsCI GGATG 3 cut(s) 19, 37, 298
BveI ACCTGC 1 cut(s) 385
Cfr10I RCCGGY 1 cut(s) 15
Cfr13I GGNCC 2 cut(s) 159, 282
Csp6I GTAC 2 cut(s) 90, 208
CviAII CATG 9 cut(s) 70, 299, 307, 341, 451, 526, 580, 584, 607
CviQI GTAC 2 cut(s) 90, 208
DdeI CTNAG 2 cut(s) 481, 668
DpnI GATC 7 cut(s) 114, 218, 316, 348, 367, 442, 546
DpnII GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
Eam1104I CTCTTC 3 cut(s) 77, 269, 452
EarI CTCTTC 3 cut(s) 77, 269, 452
Ecl136II GAGCTC 1 cut(s) 667
Eco24I GRGCYC 2 cut(s) 262, 669
Eco47I GGWCC 2 cut(s) 159, 282
Eco53kI GAGCTC 1 cut(s) 667
Eco88I CYCGRG 1 cut(s) 261
EcoICRI GAGCTC 1 cut(s) 667
EcoO109I RGGNCCY 1 cut(s) 159
EcoRII CCWGG 1 cut(s) 549
EcoT22I ATGCAT 1 cut(s) 585
EcoT38I GRGCYC 2 cut(s) 262, 669
FaeI CATG 9 cut(s) 73, 302, 310, 344, 454, 529, 583, 587, 610
FaqI GGGAC 1 cut(s) 145
FatI CATG 9 cut(s) 69, 298, 306, 340, 450, 525, 579, 583, 606
Fnu4HI GCNGC 2 cut(s) 51, 712
FokI GGATG 3 cut(s) 6, 44, 305
FriOI GRGCYC 2 cut(s) 262, 669
Fsp4HI GCNGC 2 cut(s) 51, 712
FspBI CTAG 3 cut(s) 93, 465, 498
GluI GCNGC 2 cut(s) 51, 712
GsuI CTGGAG 2 cut(s) 179, 507
HapII CCGG 3 cut(s) 16, 54, 706
Hin1II CATG 9 cut(s) 73, 302, 310, 344, 454, 529, 583, 587, 610
HinfI GANTC 3 cut(s) 100, 123, 178
HpaII CCGG 3 cut(s) 16, 54, 706
HphI GGTGA 4 cut(s) 341, 433, 539, 599
Hpy166II GTNNAC 3 cut(s) 250, 285, 424
Hpy188I TCNGA 3 cut(s) 183, 292, 622
Hpy188III TCNNGA 4 cut(s) 226, 247, 263, 706
Hpy8I GTNNAC 3 cut(s) 250, 285, 424
HpyCH4III ACNGT 1 cut(s) 641
HpyCH4IV ACGT 1 cut(s) 231
HpyCH4V TGCA 3 cut(s) 310, 374, 583
HpyF10VI GCNNNNNNNGC 4 cut(s) 16, 84, 386, 678
HpyF3I CTNAG 2 cut(s) 481, 668
HpySE526I ACGT 1 cut(s) 231
Hsp92II CATG 9 cut(s) 73, 302, 310, 344, 454, 529, 583, 587, 610
Kpn2I TCCGGA 1 cut(s) 705
Kzo9I GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
LguI GCTCTTC 1 cut(s) 77
LmnI GCTCC 2 cut(s) 133, 708
Lsp1109I GCAGC 1 cut(s) 62
LweI GCATC 1 cut(s) 28
MaeI CTAG 3 cut(s) 93, 465, 498
MaeII ACGT 1 cut(s) 231
MaeIII GTNAC 2 cut(s) 41, 635
MalI GATC 7 cut(s) 114, 218, 316, 348, 367, 442, 546
MboI GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
MboII GAAGA 5 cut(s) 94, 286, 360, 469, 554
MflI RGATCY 2 cut(s) 365, 440
MhlI GDGCHC 3 cut(s) 262, 306, 669
MmeI TCCRAC 1 cut(s) 47
Mph1103I ATGCAT 1 cut(s) 585
MroI TCCGGA 1 cut(s) 705
MroXI GAANNNNTTC 1 cut(s) 499
MseI TTAA 2 cut(s) 357, 408
MslI CAYNNNNRTG 1 cut(s) 642
MspA1I CMGCKG 3 cut(s) 199, 485, 714
MspI CCGG 3 cut(s) 16, 54, 706
MspR9I CCNGG 2 cut(s) 55, 551
MvaI CCWGG 1 cut(s) 551
MwoI GCNNNNNNNGC 4 cut(s) 16, 84, 386, 678
NciI CCSGG 1 cut(s) 55
NdeII GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
NlaIII CATG 9 cut(s) 73, 302, 310, 344, 454, 529, 583, 587, 610
NlaIV GGNNCC 3 cut(s) 6, 161, 710
NmuCI GTSAC 1 cut(s) 635
NsiI ATGCAT 1 cut(s) 585
NspI RCATGY 1 cut(s) 73
OliI CACNNNNGTG 1 cut(s) 642
PaqCI CACCTGC 1 cut(s) 385
PciSI GCTCTTC 1 cut(s) 77
PcsI WCGNNNNNNNCGW 1 cut(s) 216
PdmI GAANNNNTTC 1 cut(s) 499
PfeI GAWTC 3 cut(s) 100, 123, 178
PkrI GCNGC 2 cut(s) 52, 713
PpuMI RGGWCCY 1 cut(s) 159
Psp124BI GAGCTC 1 cut(s) 669
Psp5II RGGWCCY 1 cut(s) 159
Psp6I CCWGG 1 cut(s) 549
PspGI CCWGG 1 cut(s) 549
PspN4I GGNNCC 3 cut(s) 6, 161, 710
PspPI GGNCC 2 cut(s) 159, 282
PspPPI RGGWCCY 1 cut(s) 159
PsrI GAACNNNNNNTAC 2 cut(s) 487, 519
PsuI RGATCY 2 cut(s) 365, 440
PvuII CAGCTG 2 cut(s) 199, 485
RsaI GTAC 2 cut(s) 91, 209
RsaNI GTAC 2 cut(s) 90, 208
RseI CAYNNNNRTG 1 cut(s) 642
SacI GAGCTC 1 cut(s) 669
SapI GCTCTTC 1 cut(s) 77
SaqAI TTAA 2 cut(s) 357, 408
SatI GCNGC 2 cut(s) 51, 712
Sau3AI GATC 7 cut(s) 112, 216, 314, 346, 365, 440, 544
Sau96I GGNCC 2 cut(s) 159, 282
ScaI AGTACT 1 cut(s) 91
ScrFI CCNGG 2 cut(s) 55, 551
SduI GDGCHC 3 cut(s) 262, 306, 669
SfaNI GCATC 1 cut(s) 28
SfcI CTRYAG 2 cut(s) 108, 168
SinI GGWCC 2 cut(s) 159, 282
SmiMI CAYNNNNRTG 1 cut(s) 642
SmlI CTYRAG 2 cut(s) 128, 662
SmoI CTYRAG 2 cut(s) 128, 662
SpeI ACTAGT 1 cut(s) 497
SsiI CCGC 4 cut(s) 8, 432, 633, 712
SspMI CTAG 3 cut(s) 93, 465, 498
SstI GAGCTC 1 cut(s) 669
StyD4I CCNGG 2 cut(s) 53, 549
TaaI ACNGT 1 cut(s) 641
TaiI ACGT 1 cut(s) 234
TaqI TCGA 1 cut(s) 219
TatI WGTACW 1 cut(s) 89
TauI GCSGC 1 cut(s) 714
TfiI GAWTC 3 cut(s) 100, 123, 178
Tru1I TTAA 2 cut(s) 357, 408
Tru9I TTAA 2 cut(s) 357, 408
TseFI GTSAC 1 cut(s) 635
TseI GCWGC 1 cut(s) 50
Tsp45I GTSAC 1 cut(s) 635
TspDTI ATGAA 4 cut(s) 287, 461, 514, 623
VpaK11BI GGWCC 2 cut(s) 159, 282
XceI RCATGY 1 cut(s) 73
XmnI GAANNNNTTC 1 cut(s) 499
XspI CTAG 3 cut(s) 93, 465, 498
ZrmI AGTACT 1 cut(s) 91
Zsp2I ATGCAT 1 cut(s) 585
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.