MD02G1177400.v1.1

tRNA-dihydrouridine(16 17) synthase NAD(P)( ) -like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0015403
Physical Location & Seq
Reverse (-)
15642770 .. 15643775
1006 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1177400.v1.1.491

Sequence Viewer

Length: 633 bp
ATGAGATTTAAAATCCCCAATCTGCACCTCTTCTTTCCCAAAACCCTAAAACCCCTCACCGCCACAGAATCCCAAATCCGAACCCGAAGTCTCCCTTTTGACTCGTTGCAAGACCTCGACGACGCCCTCTGCTCCAACCACCACCAACAACAACAAGAGCAACAAACTTTCTCCTATACGCCGCCGTTGCGCTGCTTGACTGGTGAGTCGCGCATCGAGCGAGCATGGGATCAGTGGTCGAAACTGGGGCGGCCCAAACTGATTGTTGCCCCAAGTACGGGGCCCGATAGTGGACAACTCGGAGCTCCCGTTTTGGATGCTCTGCAGCAAGTACGGGGCCGACGCCGCTCATTTTCACAGAGAGTGAGAAGTACCGGGAGCAAGAATTTACCACTTGCAAGAGTATTGCAGGAGGACCGTCCATTATTTGTTCAATTTTGCGCTAATGATCCTGAAGTTCTGTTGGACGCTACAAAGAGAGTGGAGACTCATTGTGATTATGTCGACATTAATTTGGGGTGTCCTCAGCGTATTGCGAGGCGGGGGAATTACGGAGCTTTTCTGATGGATAACCTTCCTCTTATAAAATCTCTAGTAGAGAACTTGGTTCAAAACCTGAATGTGCCTGTGTAG

Protein Analysis

211

Amino Acids

23.86

Weight (kDa)

9.27

Isoelectric Point (pI)

58.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 136 - 210 4.6e-20 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0015403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67220
fragaria_vesca FvH4_1g16470
malus_domestica MD02G1177400.v1.1 MD15G1287200.v1.1
prunus_persica Prupe.6G230700_v2.0.a1
pyrus_communis pycom15g25070
rosa_chinensis RchiOBHm_Chr2g0106351
rosa_laevigata RLG00000017491
rosa_multiflora Rmu_sc0010122.1_g000004
rosa_roxburghii Rroxscaffold_2G00137260
rosa_rugosa Rorug02G0134900
rosa_samantha Rh2AG186000 Rh2BG196200 Rh2CG191000 Rh2DG192100
rosa_wichuraiana Rw2G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 584
AasI GACNNNNNNGTC 1 cut(s) 205
AccBSI CCGCTC 1 cut(s) 348
AccI GTMKAC 1 cut(s) 504
AccII CGCG 1 cut(s) 211
AciI CCGC 5 cut(s) 60, 182, 250, 346, 541
AclWI GGATC 2 cut(s) 237, 443
AcsI RAATTY 1 cut(s) 385
AcuI CTGAAG 1 cut(s) 474
AcyI GRCGYC 2 cut(s) 123, 343
AfaI GTAC 3 cut(s) 277, 333, 373
AfiI CCNNNNNNNGG 3 cut(s) 277, 278, 290
AgsI TTSAA 2 cut(s) 434, 611
AluBI AGCT 2 cut(s) 305, 557
AluI AGCT 2 cut(s) 305, 557
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 2 cut(s) 95, 479
AlwI GGATC 2 cut(s) 237, 443
AoxI GGCC 3 cut(s) 251, 281, 337
ApaI GGGCCC 1 cut(s) 285
ApeKI GCWGC 2 cut(s) 192, 325
ApoI RAATTY 1 cut(s) 385
ArsI GACNNNNNNTTYG 2 cut(s) 73, 105
AseI ATTAAT 1 cut(s) 510
AspLEI GCGC 3 cut(s) 192, 213, 443
AspS9I GGNCC 5 cut(s) 252, 281, 282, 337, 415
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 2 cut(s) 49, 215
AvaII GGWCC 1 cut(s) 415
BaeGI GKGCMC 1 cut(s) 285
BanII GRGCYC 2 cut(s) 285, 307
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 525
BbvI GCAGC 2 cut(s) 179, 337
BccI CCATC 1 cut(s) 559
BceAI ACGGC 1 cut(s) 169
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 2 cut(s) 95, 479
BfaI CTAG 1 cut(s) 593
BfmI CTRYAG 1 cut(s) 323
BisI GCNGC 5 cut(s) 182, 193, 251, 326, 346
BlsI GCNGC 5 cut(s) 183, 194, 252, 327, 347
Bme1390I CCNGG 1 cut(s) 376
Bme18I GGWCC 1 cut(s) 415
BmgT120I GGNCC 5 cut(s) 252, 281, 282, 337, 415
BmiI GGNNCC 3 cut(s) 282, 283, 338
BmrFI CCNGG 1 cut(s) 376
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 2 cut(s) 222, 307
BmuI ACTGGG 1 cut(s) 254
Bpu10I CCTNAGC 1 cut(s) 525
BpuMI CCSGG 1 cut(s) 376
BsaHI GRCGYC 2 cut(s) 123, 343
BsaXI ACNNNNNCTCC 2 cut(s) 294, 324
Bsc4I CCNNNNNNNGG 3 cut(s) 277, 278, 290
Bse1I ACTGG 2 cut(s) 205, 249
BseGI GGATG 1 cut(s) 322
BseLI CCNNNNNNNGG 3 cut(s) 277, 278, 290
BseMII CTCAG 1 cut(s) 539
BseNI ACTGG 2 cut(s) 205, 249
BseSI GKGCMC 1 cut(s) 285
BseXI GCAGC 2 cut(s) 179, 337
BsgI GTGCAG 1 cut(s) 8
Bsh1236I CGCG 1 cut(s) 211
BshFI GGCC 3 cut(s) 253, 283, 339
BsiHKAI GWGCWC 1 cut(s) 307
BsiSI CCGG 1 cut(s) 375
BslI CCNNNNNNNGG 3 cut(s) 277, 278, 290
BsmAI GTCTC 2 cut(s) 95, 479
BsnI GGCC 3 cut(s) 253, 283, 339
Bsp120I GGGCCC 1 cut(s) 281
Bsp1286I GDGCHC 2 cut(s) 285, 307
Bsp143I GATC 2 cut(s) 229, 448
BspACI CCGC 5 cut(s) 60, 182, 250, 346, 541
BspANI GGCC 3 cut(s) 253, 283, 339
BspCNI CTCAG 1 cut(s) 538
BspFNI CGCG 1 cut(s) 211
BspLI GGNNCC 3 cut(s) 282, 283, 338
BspMAI CTGCAG 1 cut(s) 327
BspPI GGATC 2 cut(s) 237, 443
BsrBI CCGCTC 1 cut(s) 348
BsrI ACTGG 2 cut(s) 205, 249
BssMI GATC 2 cut(s) 229, 448
BssNI GRCGYC 2 cut(s) 123, 343
Bst4CI ACNGT 1 cut(s) 419
Bst6I CTCTTC 1 cut(s) 35
BstACI GRCGYC 2 cut(s) 123, 343
BstC8I GCNNGC 1 cut(s) 222
BstDEI CTNAG 1 cut(s) 525
BstF5I GGATG 1 cut(s) 322
BstFNI CGCG 1 cut(s) 211
BstHHI GCGC 3 cut(s) 192, 213, 443
BstKTI GATC 2 cut(s) 232, 451
BstMAI GTCTC 2 cut(s) 95, 479
BstMBI GATC 2 cut(s) 229, 448
BstMWI GCNNNNNNNGC 3 cut(s) 187, 217, 345
BstSCI CCNGG 1 cut(s) 374
BstSFI CTRYAG 1 cut(s) 323
BstSLI GKGCMC 1 cut(s) 285
BstUI CGCG 1 cut(s) 211
BstV1I GCAGC 2 cut(s) 179, 337
BsuRI GGCC 3 cut(s) 253, 283, 339
BtsCI GGATG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 239
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 3 cut(s) 192, 213, 443
Cfr13I GGNCC 5 cut(s) 252, 281, 282, 337, 415
CseI GACGC 3 cut(s) 131, 351, 476
Csp6I GTAC 3 cut(s) 276, 332, 372
CspCI CAANNNNNGTGG 2 cut(s) 462, 497
CviAII CATG 1 cut(s) 225
CviJI RGCY 5 cut(s) 253, 283, 305, 339, 557
CviKI_1 RGCY 5 cut(s) 253, 283, 305, 339, 557
CviQI GTAC 3 cut(s) 276, 332, 372
DdeI CTNAG 1 cut(s) 525
DpnI GATC 2 cut(s) 231, 450
DpnII GATC 2 cut(s) 229, 448
DraI TTTAAA 1 cut(s) 10
DrdI GACNNNNNNGTC 1 cut(s) 205
DseDI GACNNNNNNGTC 1 cut(s) 205
Eam1104I CTCTTC 1 cut(s) 35
EarI CTCTTC 1 cut(s) 35
Ecl136II GAGCTC 1 cut(s) 305
Eco24I GRGCYC 2 cut(s) 285, 307
Eco47I GGWCC 1 cut(s) 415
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 474
EcoICRI GAGCTC 1 cut(s) 305
EcoO109I RGGNCCY 1 cut(s) 281
EcoT38I GRGCYC 2 cut(s) 285, 307
FaeI CATG 1 cut(s) 228
FaiI YATR 4 cut(s) 177, 226, 501, 584
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FatI CATG 1 cut(s) 224
FauI CCCGC 1 cut(s) 534
FblI GTMKAC 1 cut(s) 504
Fnu4HI GCNGC 5 cut(s) 182, 193, 251, 326, 346
FokI GGATG 1 cut(s) 329
FriOI GRGCYC 2 cut(s) 285, 307
Fsp4HI GCNGC 5 cut(s) 182, 193, 251, 326, 346
FspBI CTAG 1 cut(s) 593
GlaI GCGC 3 cut(s) 191, 212, 442
GluI GCNGC 5 cut(s) 182, 193, 251, 326, 346
HaeIII GGCC 3 cut(s) 253, 283, 339
HapII CCGG 1 cut(s) 375
HgaI GACGC 3 cut(s) 131, 351, 476
HhaI GCGC 3 cut(s) 192, 213, 443
Hin1I GRCGYC 2 cut(s) 123, 343
Hin1II CATG 1 cut(s) 228
Hin6I GCGC 3 cut(s) 190, 211, 441
HinP1I GCGC 3 cut(s) 190, 211, 441
HincII GTYRAC 1 cut(s) 505
HindII GTYRAC 1 cut(s) 505
HinfI GANTC 4 cut(s) 68, 101, 206, 487
HpaII CCGG 1 cut(s) 375
HphI GGTGA 2 cut(s) 49, 215
Hpy166II GTNNAC 2 cut(s) 293, 505
Hpy188I TCNGA 3 cut(s) 80, 302, 564
Hpy188III TCNNGA 1 cut(s) 452
Hpy8I GTNNAC 2 cut(s) 293, 505
Hpy99I CGWCG 3 cut(s) 122, 125, 345
HpyAV CCTTC 1 cut(s) 584
HpyCH4III ACNGT 1 cut(s) 419
HpyCH4V TGCA 5 cut(s) 25, 109, 325, 398, 409
HpyF10VI GCNNNNNNNGC 3 cut(s) 187, 217, 345
HpyF3I CTNAG 1 cut(s) 525
Hsp92I GRCGYC 2 cut(s) 123, 343
Hsp92II CATG 1 cut(s) 228
HspAI GCGC 3 cut(s) 190, 211, 441
Kzo9I GATC 2 cut(s) 229, 448
LmnI GCTCC 5 cut(s) 137, 302, 310, 378, 554
LpnPI CCDG 6 cut(s) 186, 230, 388, 395, 465, 629
Lsp1109I GCAGC 2 cut(s) 179, 337
LweI GCATC 2 cut(s) 222, 307
MaeI CTAG 1 cut(s) 593
MalI GATC 2 cut(s) 231, 450
MbiI CCGCTC 1 cut(s) 348
MboI GATC 2 cut(s) 229, 448
MboII GAAGA 1 cut(s) 22
MhlI GDGCHC 2 cut(s) 285, 307
MluCI AATT 4 cut(s) 385, 434, 511, 547
MlyI GAGTC 3 cut(s) 95, 215, 481
MmeI TCCRAC 2 cut(s) 159, 444
MnlI CCTC 8 cut(s) 38, 65, 125, 137, 406, 531, 534, 588
MseI TTAA 2 cut(s) 9, 510
MspI CCGG 1 cut(s) 375
MspR9I CCNGG 1 cut(s) 376
MvnI CGCG 1 cut(s) 211
MwoI GCNNNNNNNGC 3 cut(s) 187, 217, 345
NciI CCSGG 1 cut(s) 376
NdeII GATC 2 cut(s) 229, 448
NlaIII CATG 1 cut(s) 228
NlaIV GGNNCC 3 cut(s) 282, 283, 338
PcsI WCGNNNNNNNCGW 1 cut(s) 306
PfeI GAWTC 1 cut(s) 68
PkrI GCNGC 5 cut(s) 183, 194, 252, 327, 347
PleI GAGTC 3 cut(s) 95, 214, 481
PpsI GAGTC 3 cut(s) 95, 214, 481
PshBI ATTAAT 1 cut(s) 510
PsiI TTATAA 1 cut(s) 584
Psp124BI GAGCTC 1 cut(s) 307
PspN4I GGNNCC 3 cut(s) 282, 283, 338
PspOMI GGGCCC 1 cut(s) 281
PspPI GGNCC 5 cut(s) 252, 281, 282, 337, 415
PstI CTGCAG 1 cut(s) 327
RsaI GTAC 3 cut(s) 277, 333, 373
RsaNI GTAC 3 cut(s) 276, 332, 372
SacI GAGCTC 1 cut(s) 307
SalI GTCGAC 1 cut(s) 503
SaqAI TTAA 2 cut(s) 9, 510
SatI GCNGC 5 cut(s) 182, 193, 251, 326, 346
Sau3AI GATC 2 cut(s) 229, 448
Sau96I GGNCC 5 cut(s) 252, 281, 282, 337, 415
SchI GAGTC 3 cut(s) 95, 215, 481
ScrFI CCNGG 1 cut(s) 376
SduI GDGCHC 2 cut(s) 285, 307
SetI ASST 6 cut(s) 30, 117, 307, 559, 576, 618
SfaNI GCATC 2 cut(s) 222, 307
SfcI CTRYAG 1 cut(s) 323
SinI GGWCC 1 cut(s) 415
Sse9I AATT 4 cut(s) 385, 434, 511, 547
SsiI CCGC 5 cut(s) 60, 182, 250, 346, 541
SspMI CTAG 1 cut(s) 593
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 1 cut(s) 374
TaaI ACNGT 1 cut(s) 419
TaqI TCGA 4 cut(s) 117, 216, 239, 504
TasI AATT 4 cut(s) 385, 434, 511, 547
TauI GCSGC 3 cut(s) 184, 253, 348
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 2 cut(s) 9, 510
Tru9I TTAA 2 cut(s) 9, 510
TscAI CASTG 1 cut(s) 239
TseI GCWGC 2 cut(s) 192, 325
TspGWI ACGGA 1 cut(s) 567
TspRI CASTG 1 cut(s) 239
VpaK11BI GGWCC 1 cut(s) 415
VspI ATTAAT 1 cut(s) 510
XapI RAATTY 1 cut(s) 385
XmiI GTMKAC 1 cut(s) 504
XspI CTAG 1 cut(s) 593
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.