Rh2BG196200

tRNA-dihydrouridine(16 17) synthase NAD(P)( ) -like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0015403
Physical Location & Seq
Reverse (-)
17884007 .. 17886169
2163 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG196200.1

Sequence Viewer

Length: 1275 bp
ATGAAATTCAAACTTCCCAATCTACCCCTCCCCTCCCTCAAAACCCTTCCCCTAAAACCCAAGTCCCAAATGGCCTCACTTCTCACCGCCGCCGAGCCCCAAACCCGAATTCCTCACCCGTCGGAAGACCTCGATGACGTCCTCTGCTCCTCCCAGCAACAGCAGCAGCAGTTGGATTTGGCTGAAACGGCGCCGTTTCGGAACTTGAGCGGCGAGTCGCGAATTGAGCGAGCATGGGCTCACTGGACGAAGCTGGGCCGGCCCAAGTTGATCGTGGCTCCGATGGTGGACAACTCCGAGCTCCCGTTTCGGATGCTCTGCCGCAAGTACGGCGCCGACGCTGCCTACACGCCCATGCTTCACTCTCGGATTTTTACCGAGACCGAGAAGTATCGGAACCAAGAATTTACCACTTGCAAGGAGGATCGTCCGTTGTTTGTTCAATTCTGTGCTAATGATCCTGAGGTTTTACTGGAAGCTGCAAAGAGAGTGGAGCCTTACTGTGATTATGTTGACATTAATTTGGGGTGTCCTCAGCGAATTGCCAGGCGGGGGAACTATGGAGCTTTTCTGATGGATAAGCTTCCTCTTGTAAAATCTCTAGTAGAAAACTTGGTTGCAAATCTTTATGTCCCTGTTTCATGCAAAATCCGTATCTTCCCGAACTTGGATGATACAATTCAGTATGCTAGGATGCTAGAGGATGCCGGTTGCTCTCTTTTAGCCGTCCATGGCCGAACAAGAGACGAGAAAGACCAGAAGAAAGTGCGGGCCAACTGGGATGCCATCAAGGCTGTGAAGAATTCTGTCAAAATACCTGTGATTGCCAATGGGAACATAAGGCACCTGGATGATGCGCACGACTGTTTGAAATACACAGGTGCTGATGGGGTACTTTCTGCTGAGTCTCTTCTTGAAAATCCAGCACTTTTTGCTGGTTTTCGAACTGCTGAATGGGTTGAGGAGGGTGAAGAATGCAATAAAGATCGGAAACTGGACCAGGCAGATCTTTTGGTGGAGTATCTTAAGCTTTGTGAACAATACCCTGTACCTTGGCGTATGATTCGTGCTCATGTGCACAAGATGCTGGGAGACTGGTTCAGAATGCATCCACATGTAAGAGAAGATTTTAATGCACAGCACATTCTCACTTTTGAGTTTCTCTATAACATGGTGGATCGGCTTAGAGAGCTGGGTAAAGGAACTCCCCTTCATCTTAAGGATACTAATGCTGCAAGTATTGCAGCAAATGGGCTGGTTGTGTCAGCTGTATGA

Protein Analysis

424

Amino Acids

48.27

Weight (kDa)

6.87

Isoelectric Point (pI)

32.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dus PF01207 92 - 377 1.6e-77 Dihydrouridine synthase (Dus)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0015403)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G67220
fragaria_vesca FvH4_1g16470
malus_domestica MD02G1177400.v1.1 MD15G1287200.v1.1
prunus_persica Prupe.6G230700_v2.0.a1
pyrus_communis pycom15g25070
rosa_chinensis RchiOBHm_Chr2g0106351
rosa_laevigata RLG00000017491
rosa_multiflora Rmu_sc0010122.1_g000004
rosa_roxburghii Rroxscaffold_2G00137260
rosa_rugosa Rorug02G0134900
rosa_samantha Rh2AG186000 Rh2BG196200 Rh2CG191000 Rh2DG192100
rosa_wichuraiana Rw2G014500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 141
Acc16I TGCGCA 1 cut(s) 858
AccB1I GGYRCC 3 cut(s) 190, 332, 843
AccBSI CCGCTC 1 cut(s) 210
AccII CGCG 1 cut(s) 220
AciI CCGC 6 cut(s) 87, 90, 210, 322, 550, 769
AclWI GGATC 3 cut(s) 432, 452, 1185
AcoI YGGCCR 1 cut(s) 733
AcsI RAATTY 4 cut(s) 5, 108, 404, 802
AcyI GRCGYC 3 cut(s) 138, 191, 333
AfaI GTAC 3 cut(s) 329, 894, 1050
AfiI CCNNNNNNNGG 2 cut(s) 552, 667
AflII CTTAAG 2 cut(s) 1025, 1217
AflIII ACRYGT 1 cut(s) 1114
AgsI TTSAA 4 cut(s) 10, 443, 871, 917
AjnI CCWGG 3 cut(s) 545, 846, 999
AjuI GAANNNNNNNTTGG 2 cut(s) 93, 125
AluBI AGCT 8 cut(s) 253, 301, 479, 566, 583, 1030, 1192, 1268
AluI AGCT 8 cut(s) 253, 301, 479, 566, 583, 1030, 1192, 1268
Alw21I GWGCWC 3 cut(s) 303, 1072, 1080
Alw26I GTCTC 4 cut(s) 374, 738, 912, 1086
Alw44I GTGCAC 1 cut(s) 1076
AlwI GGATC 3 cut(s) 432, 452, 1185
AlwNI CAGNNNCTG 1 cut(s) 884
AoxI GGCC 5 cut(s) 72, 256, 260, 733, 771
ApaLI GTGCAC 1 cut(s) 1076
ApeKI GCWGC 6 cut(s) 163, 166, 341, 479, 1232, 1244
ApoI RAATTY 4 cut(s) 5, 108, 404, 802
AseI ATTAAT 1 cut(s) 519
AspLEI GCGC 3 cut(s) 193, 335, 859
AspS9I GGNCC 4 cut(s) 256, 261, 771, 997
AsuHPI GGTGA 3 cut(s) 76, 107, 980
AsuII TTCGAA 1 cut(s) 943
AvaII GGWCC 1 cut(s) 997
AxyI CCTNAGG 1 cut(s) 462
BaeGI GKGCMC 1 cut(s) 1080
BanI GGYRCC 3 cut(s) 190, 332, 843
BanII GRGCYC 3 cut(s) 99, 241, 303
BbsI GAAGAC 1 cut(s) 132
Bbv12I GWGCWC 3 cut(s) 303, 1072, 1080
BbvCI CCTCAGC 1 cut(s) 534
BbvI GCAGC 6 cut(s) 175, 178, 328, 466, 1219, 1256
BccI CCATC 4 cut(s) 277, 568, 794, 881
BceAI ACGGC 4 cut(s) 178, 204, 346, 710
BciT130I CCWGG 3 cut(s) 547, 848, 1001
BciVI GTATCC 1 cut(s) 1216
BcoDI GTCTC 4 cut(s) 374, 738, 912, 1086
BfaI CTAG 3 cut(s) 602, 690, 698
BfoI RGCGCY 2 cut(s) 194, 336
BfrI CTTAAG 2 cut(s) 1025, 1217
BfuI GTATCC 1 cut(s) 1216
BglI GCCNNNNNGGC 1 cut(s) 791
BglII AGATCT 1 cut(s) 1006
BisI GCNGC 9 cut(s) 90, 164, 167, 211, 322, 342, 480, 1233, 1245
BlsI GCNGC 9 cut(s) 91, 165, 168, 212, 323, 343, 481, 1234, 1246
Bme1390I CCNGG 3 cut(s) 547, 848, 1001
Bme18I GGWCC 1 cut(s) 997
BmgT120I GGNCC 4 cut(s) 256, 261, 771, 997
BmiI GGNNCC 6 cut(s) 192, 279, 334, 398, 495, 845
BmrFI CCNGG 3 cut(s) 547, 848, 1001
BmrI ACTGGG 1 cut(s) 787
BmsI GCATC 7 cut(s) 303, 684, 694, 772, 844, 1074, 1117
BmuI ACTGGG 1 cut(s) 787
BpiI GAAGAC 1 cut(s) 132
Bpu10I CCTNAGC 1 cut(s) 534
Bpu14I TTCGAA 1 cut(s) 943
BpuEI CTTGAG 1 cut(s) 226
BsaHI GRCGYC 3 cut(s) 138, 191, 333
BsaI GGTCTC 1 cut(s) 374
BsaJI CCNNGG 2 cut(s) 730, 1052
Bsc4I CCNNNNNNNGG 2 cut(s) 552, 667
Bse118I RCCGGY 2 cut(s) 258, 707
Bse1I ACTGG 5 cut(s) 248, 477, 782, 999, 1100
Bse21I CCTNAGG 1 cut(s) 462
BseBI CCWGG 3 cut(s) 547, 848, 1001
BseDI CCNNGG 2 cut(s) 730, 1052
BseGI GGATG 7 cut(s) 318, 676, 699, 709, 787, 856, 1108
BseLI CCNNNNNNNGG 2 cut(s) 552, 667
BseMII CTCAG 3 cut(s) 453, 548, 894
BseNI ACTGG 5 cut(s) 248, 477, 782, 999, 1100
BseRI GAGGAG 2 cut(s) 139, 977
BseSI GKGCMC 1 cut(s) 1080
BseXI GCAGC 6 cut(s) 175, 178, 328, 466, 1219, 1256
BseYI CCCAGC 4 cut(s) 153, 253, 1087, 1192
Bsh1236I CGCG 1 cut(s) 220
BshFI GGCC 5 cut(s) 74, 258, 262, 735, 773
BshNI GGYRCC 3 cut(s) 190, 332, 843
BsiHKAI GWGCWC 3 cut(s) 303, 1072, 1080
BsiSI CCGG 2 cut(s) 259, 708
BslFI GGGAC 2 cut(s) 49, 617
BslI CCNNNNNNNGG 2 cut(s) 552, 667
BsmAI GTCTC 4 cut(s) 374, 738, 912, 1086
BsmBI CGTCTC 1 cut(s) 738
BsmFI GGGAC 2 cut(s) 49, 617
BsmI GAATGC 2 cut(s) 980, 1110
BsnI GGCC 5 cut(s) 74, 258, 262, 735, 773
Bso31I GGTCTC 1 cut(s) 374
Bsp119I TTCGAA 1 cut(s) 943
Bsp1286I GDGCHC 5 cut(s) 99, 241, 303, 1072, 1080
Bsp143I GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
Bsp19I CCATGG 1 cut(s) 730
Bsp68I TCGCGA 1 cut(s) 220
BspACI CCGC 6 cut(s) 87, 90, 210, 322, 550, 769
BspANI GGCC 5 cut(s) 74, 258, 262, 735, 773
BspCNI CTCAG 3 cut(s) 454, 547, 895
BspFNI CGCG 1 cut(s) 220
BspLI GGNNCC 6 cut(s) 192, 279, 334, 398, 495, 845
BspPI GGATC 3 cut(s) 432, 452, 1185
BspT104I TTCGAA 1 cut(s) 943
BspT107I GGYRCC 3 cut(s) 190, 332, 843
BspTI CTTAAG 2 cut(s) 1025, 1217
BspTNI GGTCTC 1 cut(s) 374
BsrBI CCGCTC 1 cut(s) 210
BsrFI RCCGGY 2 cut(s) 258, 707
BsrI ACTGG 5 cut(s) 248, 477, 782, 999, 1100
BssAI RCCGGY 2 cut(s) 258, 707
BssECI CCNNGG 2 cut(s) 730, 1052
BssMI GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
BssNI GRCGYC 3 cut(s) 138, 191, 333
BssT1I CCWWGG 2 cut(s) 730, 1052
Bst2UI CCWGG 3 cut(s) 547, 848, 1001
Bst4CI ACNGT 2 cut(s) 503, 866
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 3 cut(s) 138, 191, 333
BstAFI CTTAAG 2 cut(s) 1025, 1217
BstAPI GCANNNNNTGC 3 cut(s) 932, 1084, 1241
BstBI TTCGAA 1 cut(s) 943
BstC8I GCNNGC 3 cut(s) 231, 260, 771
BstDEI CTNAG 4 cut(s) 462, 534, 903, 1184
BstDSI CCRYGG 1 cut(s) 730
BstF5I GGATG 7 cut(s) 318, 676, 699, 709, 787, 856, 1108
BstFNI CGCG 1 cut(s) 220
BstH2I RGCGCY 2 cut(s) 194, 336
BstHHI GCGC 3 cut(s) 193, 335, 859
BstKTI GATC 6 cut(s) 273, 427, 460, 988, 1009, 1180
BstMAI GTCTC 4 cut(s) 374, 738, 912, 1086
BstMBI GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
BstNI CCWGG 3 cut(s) 547, 848, 1001
BstNSI RCATGY 1 cut(s) 1118
BstSCI CCNGG 3 cut(s) 545, 846, 999
BstSLI GKGCMC 1 cut(s) 1080
BstUI CGCG 1 cut(s) 220
BstV1I GCAGC 6 cut(s) 175, 178, 328, 466, 1219, 1256
BstV2I GAAGAC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 1006
BstYI RGATCY 1 cut(s) 1006
Bsu36I CCTNAGG 1 cut(s) 462
BsuI GTATCC 1 cut(s) 1216
BsuRI GGCC 5 cut(s) 74, 258, 262, 735, 773
BtgI CCRYGG 1 cut(s) 730
BtsCI GGATG 7 cut(s) 318, 676, 699, 709, 787, 856, 1108
BtsIMutI CAGTG 1 cut(s) 241
BtuMI TCGCGA 1 cut(s) 220
Cac8I GCNNGC 3 cut(s) 231, 260, 771
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 3 cut(s) 193, 335, 859
Cfr10I RCCGGY 2 cut(s) 258, 707
Cfr13I GGNCC 4 cut(s) 256, 261, 771, 997
CseI GACGC 1 cut(s) 347
Csp6I GTAC 3 cut(s) 328, 893, 1049
CspCI CAANNNNNGTGG 2 cut(s) 471, 506
CviAII CATG 7 cut(s) 234, 355, 642, 731, 1073, 1115, 1171
CviQI GTAC 3 cut(s) 328, 893, 1049
DdeI CTNAG 4 cut(s) 462, 534, 903, 1184
DinI GGCGCC 2 cut(s) 192, 334
DpnI GATC 6 cut(s) 272, 426, 459, 987, 1008, 1179
DpnII GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
EaeI YGGCCR 1 cut(s) 733
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
Ecl136II GAGCTC 1 cut(s) 301
Eco130I CCWWGG 2 cut(s) 730, 1052
Eco24I GRGCYC 3 cut(s) 99, 241, 303
Eco31I GGTCTC 1 cut(s) 374
Eco47I GGWCC 1 cut(s) 997
Eco53kI GAGCTC 1 cut(s) 301
Eco81I CCTNAGG 1 cut(s) 462
EcoICRI GAGCTC 1 cut(s) 301
EcoRI GAATTC 2 cut(s) 108, 802
EcoRII CCWGG 3 cut(s) 545, 846, 999
EcoT14I CCWWGG 2 cut(s) 730, 1052
EcoT22I ATGCAT 1 cut(s) 1110
EcoT38I GRGCYC 3 cut(s) 99, 241, 303
EgeI GGCGCC 2 cut(s) 192, 334
EheI GGCGCC 2 cut(s) 192, 334
ErhI CCWWGG 2 cut(s) 730, 1052
Esp3I CGTCTC 1 cut(s) 738
FaeI CATG 7 cut(s) 237, 358, 645, 734, 1076, 1118, 1174
FaqI GGGAC 2 cut(s) 49, 617
FatI CATG 7 cut(s) 233, 354, 641, 730, 1072, 1114, 1170
FauI CCCGC 2 cut(s) 543, 762
Fnu4HI GCNGC 9 cut(s) 90, 164, 167, 211, 322, 342, 480, 1233, 1245
FokI GGATG 7 cut(s) 325, 683, 706, 716, 794, 863, 1095
FriOI GRGCYC 3 cut(s) 99, 241, 303
FseI GGCCGGCC 1 cut(s) 262
Fsp4HI GCNGC 9 cut(s) 90, 164, 167, 211, 322, 342, 480, 1233, 1245
FspAI RTGCGCAY 1 cut(s) 858
FspBI CTAG 3 cut(s) 602, 690, 698
FspI TGCGCA 1 cut(s) 858
GlaI GCGC 3 cut(s) 192, 334, 858
GluI GCNGC 9 cut(s) 90, 164, 167, 211, 322, 342, 480, 1233, 1245
GsaI CCCAGC 4 cut(s) 157, 257, 1091, 1196
HaeII RGCGCY 2 cut(s) 194, 336
HaeIII GGCC 5 cut(s) 74, 258, 262, 735, 773
HapII CCGG 2 cut(s) 259, 708
HgaI GACGC 1 cut(s) 347
HhaI GCGC 3 cut(s) 193, 335, 859
Hin1I GRCGYC 3 cut(s) 138, 191, 333
Hin1II CATG 7 cut(s) 237, 358, 645, 734, 1076, 1118, 1174
Hin6I GCGC 3 cut(s) 191, 333, 857
HinP1I GCGC 3 cut(s) 191, 333, 857
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 581, 1028
HinfI GANTC 3 cut(s) 215, 905, 1063
HpaII CCGG 2 cut(s) 259, 708
HphI GGTGA 3 cut(s) 76, 107, 980
Hpy166II GTNNAC 4 cut(s) 289, 514, 1037, 1078
Hpy188III TCNNGA 4 cut(s) 219, 461, 661, 914
Hpy8I GTNNAC 4 cut(s) 289, 514, 1037, 1078
Hpy99I CGWCG 2 cut(s) 124, 341
HpyAV CCTTC 2 cut(s) 56, 1220
HpyCH4III ACNGT 2 cut(s) 503, 866
HpyCH4IV ACGT 1 cut(s) 138
HpyF3I CTNAG 4 cut(s) 462, 534, 903, 1184
HpySE526I ACGT 1 cut(s) 138
Hsp92I GRCGYC 3 cut(s) 138, 191, 333
Hsp92II CATG 7 cut(s) 237, 358, 645, 734, 1076, 1118, 1174
HspAI GCGC 3 cut(s) 191, 333, 857
KasI GGCGCC 2 cut(s) 190, 332
KroI GCCGGC 1 cut(s) 258
KroNI GCCGGC 1 cut(s) 260
Kzo9I GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
LmnI GCTCC 5 cut(s) 152, 283, 306, 493, 563
Lsp1109I GCAGC 6 cut(s) 175, 178, 328, 466, 1219, 1256
LweI GCATC 7 cut(s) 303, 684, 694, 772, 844, 1074, 1117
MaeI CTAG 3 cut(s) 602, 690, 698
MaeII ACGT 1 cut(s) 138
MalI GATC 6 cut(s) 272, 426, 459, 987, 1008, 1179
MbiI CCGCTC 1 cut(s) 210
MboI GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
MboII GAAGA 7 cut(s) 137, 649, 772, 811, 902, 983, 1136
MflI RGATCY 1 cut(s) 1006
MhlI GDGCHC 5 cut(s) 99, 241, 303, 1072, 1080
MluCI AATT 9 cut(s) 5, 108, 222, 404, 443, 520, 540, 678, 802
Mly113I GGCGCC 2 cut(s) 191, 333
MlyI GAGTC 2 cut(s) 224, 914
MmeI TCCRAC 2 cut(s) 102, 153
Mph1103I ATGCAT 1 cut(s) 1110
MroNI GCCGGC 1 cut(s) 258
MseI TTAA 4 cut(s) 519, 1026, 1131, 1218
MslI CAYNNNNRTG 3 cut(s) 353, 849, 1113
MspA1I CMGCKG 1 cut(s) 1268
MspCI CTTAAG 2 cut(s) 1025, 1217
MspI CCGG 2 cut(s) 259, 708
MspR9I CCNGG 3 cut(s) 547, 848, 1001
Mva1269I GAATGC 2 cut(s) 980, 1110
MvaI CCWGG 3 cut(s) 547, 848, 1001
MvnI CGCG 1 cut(s) 220
NaeI GCCGGC 1 cut(s) 260
NarI GGCGCC 2 cut(s) 191, 333
NcoI CCATGG 1 cut(s) 730
NdeII GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
NgoMIV GCCGGC 1 cut(s) 258
NlaIII CATG 7 cut(s) 237, 358, 645, 734, 1076, 1118, 1174
NlaIV GGNNCC 6 cut(s) 192, 279, 334, 398, 495, 845
NmeAIII GCCGAG 1 cut(s) 118
NruI TCGCGA 1 cut(s) 220
NsbI TGCGCA 1 cut(s) 858
NsiI ATGCAT 1 cut(s) 1110
NspI RCATGY 1 cut(s) 1118
NspV TTCGAA 1 cut(s) 943
PciI ACATGT 1 cut(s) 1114
PctI GAATGC 2 cut(s) 980, 1110
PdiI GCCGGC 1 cut(s) 260
PfeI GAWTC 1 cut(s) 1063
PkrI GCNGC 9 cut(s) 91, 165, 168, 212, 323, 343, 481, 1234, 1246
PleI GAGTC 2 cut(s) 223, 913
PluTI GGCGCC 2 cut(s) 194, 336
PpsI GAGTC 2 cut(s) 223, 913
PscI ACATGT 1 cut(s) 1114
PshBI ATTAAT 1 cut(s) 519
Psp124BI GAGCTC 1 cut(s) 303
Psp6I CCWGG 3 cut(s) 545, 846, 999
PspFI CCCAGC 4 cut(s) 153, 253, 1087, 1192
PspGI CCWGG 3 cut(s) 545, 846, 999
PspN4I GGNNCC 6 cut(s) 192, 279, 334, 398, 495, 845
PspPI GGNCC 4 cut(s) 256, 261, 771, 997
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 1 cut(s) 1006
PvuII CAGCTG 1 cut(s) 1268
RigI GGCCGGCC 1 cut(s) 262
RruI TCGCGA 1 cut(s) 220
RsaI GTAC 3 cut(s) 329, 894, 1050
RsaNI GTAC 3 cut(s) 328, 893, 1049
RseI CAYNNNNRTG 3 cut(s) 353, 849, 1113
SacI GAGCTC 1 cut(s) 303
SaqAI TTAA 4 cut(s) 519, 1026, 1131, 1218
SatI GCNGC 9 cut(s) 90, 164, 167, 211, 322, 342, 480, 1233, 1245
Sau3AI GATC 6 cut(s) 270, 424, 457, 985, 1006, 1177
Sau96I GGNCC 4 cut(s) 256, 261, 771, 997
SchI GAGTC 2 cut(s) 224, 914
ScrFI CCNGG 3 cut(s) 547, 848, 1001
SduI GDGCHC 5 cut(s) 99, 241, 303, 1072, 1080
SfaNI GCATC 7 cut(s) 303, 684, 694, 772, 844, 1074, 1117
SfoI GGCGCC 2 cut(s) 192, 334
SfuI TTCGAA 1 cut(s) 943
SinI GGWCC 1 cut(s) 997
SmiMI CAYNNNNRTG 3 cut(s) 353, 849, 1113
SmlI CTYRAG 3 cut(s) 205, 1025, 1217
SmoI CTYRAG 3 cut(s) 205, 1025, 1217
Sse9I AATT 9 cut(s) 5, 108, 222, 404, 443, 520, 540, 678, 802
SsiI CCGC 6 cut(s) 87, 90, 210, 322, 550, 769
SspDI GGCGCC 2 cut(s) 190, 332
SspMI CTAG 3 cut(s) 602, 690, 698
SstI GAGCTC 1 cut(s) 303
StyD4I CCNGG 3 cut(s) 545, 846, 999
StyI CCWWGG 2 cut(s) 730, 1052
TaaI ACNGT 2 cut(s) 503, 866
TaiI ACGT 1 cut(s) 141
TaqI TCGA 2 cut(s) 132, 943
TaqII GACCGA 1 cut(s) 398
TasI AATT 9 cut(s) 5, 108, 222, 404, 443, 520, 540, 678, 802
TauI GCSGC 3 cut(s) 92, 213, 324
TfiI GAWTC 1 cut(s) 1063
Tru1I TTAA 4 cut(s) 519, 1026, 1131, 1218
Tru9I TTAA 4 cut(s) 519, 1026, 1131, 1218
TscAI CASTG 1 cut(s) 248
TseI GCWGC 6 cut(s) 163, 166, 341, 479, 1232, 1244
TspDTI ATGAA 3 cut(s) 17, 630, 1202
TspGWI ACGGA 2 cut(s) 420, 641
TspRI CASTG 1 cut(s) 248
Vha464I CTTAAG 2 cut(s) 1025, 1217
VneI GTGCAC 1 cut(s) 1076
VpaK11BI GGWCC 1 cut(s) 997
VspI ATTAAT 1 cut(s) 519
XapI RAATTY 4 cut(s) 5, 108, 404, 802
XceI RCATGY 1 cut(s) 1118
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 271
XspI CTAG 3 cut(s) 602, 690, 698
ZraI GACGTC 1 cut(s) 139
Zsp2I ATGCAT 1 cut(s) 1110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.