FvH4_1g00060

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014625
Physical Location & Seq
Forward (+)
39517 .. 45833
6317 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g00060.t2

Sequence Viewer

Length: 1434 bp
ATGCTTTTGGGGTTTATATCACTCCTGCTGACGGTGTTCCAAGGACCTATAGAAAAGATCTGCATATCAAAGAAACTGGCCAGTCATTGGCTGCCTTGTAATGAAGAAGAGTCGGAGTCAACGTCAACGACTGCCCATTTCCAGACCAGCAGCTTCTTCACTAGTAACTACTATTCTCGGCATCTGCTGCTTTTTCCAGCCGGGACTGCCCGTCGCCTTCTTGCAGATGACTCCGAGACCTCTAAATCCGGTTCCACCGGCACCTGTTCAGAGGGGAAGGTCCCATTGCTATCCACGACTGCATTGCATCATCTACATATTTTCATATTTGTGCTAGCTGTGGTGCATGTGTCGTTCTGTGCTCTAACCATTATTTTTGGAGGAATTAAGATACGTCAATGGAAACAATGGGAAGATTCTATAGTGAGAAAAGAGTACAATCCAGAAGAAGTTTTGACAGAGAAGTTCACTGCCGTCCAAGACCATGAATTTATCAGGGGTCGTTTTCTGGGTATCGGAAAGAATCAAGCTTTTCTGGGTTGGTTGATTGCATTTTTCAAGCAATTTTATGGGTCTGTGACCAAAACAGATTATATGACAATGCGACTAGGCTTCATCAAGACACATTGCAAGAGAAATCCAAATTTTAATTTCCACAAGTACATGGTACGTGCCCTCGAGGCAGATTTCAAGAGAGTCGTTGGAATAAGTTGGTACCTCTGGATGTTTGTAGTCCTTTTCTTGTTGCTGAATGTTGCTGGTTGGCATGCCTATTTCTGGATAGCATTCGTTCCGTTCATTCTTTTACTTGCTGTTGGCACCAAGTTGGAGCATGTTATAATCCAGTTAGCCCATGATGTCGCCGAGAAGCACATCGCAATAGAAGGAGACTTGGTGGTTCAACCTTCAGACGATCACTTCTGGTTCCGCAGGCCTCGATTGGTTCTCTTACTCATTCATATCATCTTGTTCCAAAATTCTTTTGAACTCGCACTTTTCTTCTGGATATGGATTCAATATAAATTTGATTCCTGCATGATGGGGAAAATTGGTTACGTTATCCCAAGACTTGTAATCGGAGCATTTGTCCAGTTCGTCTGCAGCTACAGTACGTTGCCACTGTACGCAATTGTCACACAGATGGGAACATCTTTTAAGAACGCAATATTCGACGAACACGTACAAGTGGGACTTGCTGGGTGGGCTAAATCGGCAAAAAAGCACCAGGCGGCTTTGAGGAAGGCAGCTACTAATGGTAACGGCAGTAGTAGTGGTTCTAGCAAAGTCAGTCATAGCAGCAAATACAAATTGCACAAAGAAGAGGCAACTTCTGTGTCCAACACAACATCGGGGAAACTTGAATTGGCTCAAGTTTCCGTTCATGCGGGAGAAATTCAGCCTCATCAGCAGCCACCCGCCAATGATCATGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

478

Amino Acids

54.08

Weight (kDa)

9.12

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 5 - 494 2.3e-192 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0014625)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g00060 FvH4_1g00060 FvH4_1g00060 FvH4_3g16430
malus_domestica MD02G1001300.v1.1
prunus_persica Prupe.7G271400_v2.0.a1 Prupe.7G271400_v2.0.a1
pyrus_communis pycom290g00060
rosa_chinensis RchiOBHm_Chr2g0084281
rosa_laevigata RLG00000015606
rosa_multiflora Rmu_sc0003129.1_g000012
rosa_rugosa Rorug01G0452400
rosa_samantha Rh2AG000800 Rh2BG000700 Rh2CG000600 Rh2DG000800
rosa_wichuraiana Rw2G000060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 839
AbsI CCTCGAGG 1 cut(s) 677
Acc65I GGTACC 1 cut(s) 714
AccB1I GGYRCC 3 cut(s) 260, 714, 818
AccB7I CCANNNNNTGG 1 cut(s) 87
AciI CCGC 4 cut(s) 928, 1229, 1385, 1416
AcoI YGGCCR 1 cut(s) 78
AcsI RAATTY 5 cut(s) 488, 643, 976, 1022, 1392
AcuI CTGAAG 1 cut(s) 891
AfaI GTAC 7 cut(s) 437, 662, 669, 716, 1111, 1124, 1182
AfiI CCNNNNNNNGG 3 cut(s) 31, 87, 777
AflIII ACRYGT 1 cut(s) 1177
AgsI TTSAA 6 cut(s) 559, 691, 902, 986, 1016, 1361
AhdI GACNNNNNGTC 1 cut(s) 210
AhlI ACTAGT 1 cut(s) 161
AjnI CCWGG 1 cut(s) 1224
AjuI GAANNNNNNNTTGG 2 cut(s) 1346, 1378
AluBI AGCT 5 cut(s) 153, 338, 530, 1104, 1247
AluI AGCT 5 cut(s) 153, 338, 530, 1104, 1247
Alw21I GWGCWC 1 cut(s) 364
Alw26I GTCTC 2 cut(s) 230, 882
Ama87I CYCGRG 1 cut(s) 677
AoxI GGCC 2 cut(s) 78, 932
ApeKI GCWGC 7 cut(s) 91, 150, 187, 1101, 1244, 1296, 1408
ApoI RAATTY 5 cut(s) 488, 643, 976, 1022, 1392
Asp718I GGTACC 1 cut(s) 714
AspS9I GGNCC 2 cut(s) 44, 280
AsuC2I CCSGG 1 cut(s) 202
AsuNHI GCTAGC 1 cut(s) 334
AvaI CYCGRG 1 cut(s) 677
AvaII GGWCC 2 cut(s) 44, 280
BaeGI GKGCMC 1 cut(s) 676
BalI TGGCCA 1 cut(s) 80
BanI GGYRCC 3 cut(s) 260, 714, 818
Bbv12I GWGCWC 1 cut(s) 364
BbvI GCAGC 7 cut(s) 78, 162, 174, 1113, 1256, 1308, 1420
BccI CCATC 2 cut(s) 1033, 1135
BceAI ACGGC 2 cut(s) 458, 1276
BcgI CGANNNNNNTGC 2 cut(s) 286, 320
BciT130I CCWGG 1 cut(s) 1226
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 1 cut(s) 202
BcoDI GTCTC 2 cut(s) 230, 882
BcuI ACTAGT 1 cut(s) 161
BfaI CTAG 4 cut(s) 162, 335, 608, 1278
BfmI CTRYAG 4 cut(s) 48, 420, 1099, 1105
BglI GCCNNNNNGGC 1 cut(s) 680
BglII AGATCT 1 cut(s) 57
BisI GCNGC 8 cut(s) 92, 151, 188, 1102, 1230, 1245, 1297, 1409
BlsI GCNGC 8 cut(s) 93, 152, 189, 1103, 1231, 1246, 1298, 1410
Bme1390I CCNGG 2 cut(s) 202, 1226
Bme18I GGWCC 2 cut(s) 44, 280
BmeRI GACNNNNNGTC 1 cut(s) 210
BmeT110I CYCGRG 1 cut(s) 677
BmgT120I GGNCC 2 cut(s) 44, 280
BmiI GGNNCC 6 cut(s) 253, 262, 282, 716, 820, 926
BmrFI CCNGG 2 cut(s) 202, 1226
BmsI GCATC 2 cut(s) 190, 316
BmtI GCTAGC 1 cut(s) 338
BpuEI CTTGAG 1 cut(s) 1353
BpuMI CCSGG 1 cut(s) 202
BsaAI YACGTR 2 cut(s) 671, 1180
BsaI GGTCTC 1 cut(s) 230
BsaJI CCNNGG 1 cut(s) 40
BsaWI WCCGGW 1 cut(s) 248
BsaXI ACNNNNNCTCC 6 cut(s) 107, 137, 879, 909, 1071, 1101
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 87, 777
Bse118I RCCGGY 1 cut(s) 257
Bse1I ACTGG 4 cut(s) 81, 81, 844, 1090
Bse3DI GCAATG 3 cut(s) 284, 302, 625
BseBI CCWGG 1 cut(s) 1226
BseDI CCNNGG 1 cut(s) 40
BseGI GGATG 1 cut(s) 729
BseLI CCNNNNNNNGG 3 cut(s) 31, 87, 777
BseMI GCAATG 3 cut(s) 284, 302, 625
BseNI ACTGG 4 cut(s) 81, 81, 844, 1090
BseSI GKGCMC 1 cut(s) 676
BseXI GCAGC 7 cut(s) 78, 162, 174, 1113, 1256, 1308, 1420
BseYI CCCAGC 1 cut(s) 1196
BshFI GGCC 2 cut(s) 80, 934
BshNI GGYRCC 3 cut(s) 260, 714, 818
BsiHKAI GWGCWC 1 cut(s) 364
BsiHKCI CYCGRG 1 cut(s) 677
BsiSI CCGG 3 cut(s) 201, 249, 258
BslFI GGGAC 3 cut(s) 217, 266, 1203
BslI CCNNNNNNNGG 3 cut(s) 31, 87, 777
BsmAI GTCTC 2 cut(s) 230, 882
BsmFI GGGAC 3 cut(s) 217, 266, 1203
BsmI GAATGC 1 cut(s) 785
BsnI GGCC 2 cut(s) 80, 934
Bso31I GGTCTC 1 cut(s) 230
BsoBI CYCGRG 1 cut(s) 677
Bsp1286I GDGCHC 2 cut(s) 364, 676
Bsp143I GATC 3 cut(s) 57, 913, 1423
BspACI CCGC 4 cut(s) 928, 1229, 1385, 1416
BspANI GGCC 2 cut(s) 80, 934
BspLI GGNNCC 6 cut(s) 253, 262, 282, 716, 820, 926
BspMAI CTGCAG 1 cut(s) 1103
BspOI GCTAGC 1 cut(s) 338
BspT107I GGYRCC 3 cut(s) 260, 714, 818
BspTNI GGTCTC 1 cut(s) 230
BsrDI GCAATG 3 cut(s) 284, 302, 625
BsrFI RCCGGY 1 cut(s) 257
BsrI ACTGG 4 cut(s) 81, 81, 844, 1090
BssAI RCCGGY 1 cut(s) 257
BssECI CCNNGG 1 cut(s) 40
BssMI GATC 3 cut(s) 57, 913, 1423
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 1 cut(s) 1226
Bst4CI ACNGT 3 cut(s) 34, 1109, 1122
Bst6I CTCTTC 2 cut(s) 102, 1314
BstAPI GCANNNNNTGC 1 cut(s) 187
BstBAI YACGTR 2 cut(s) 671, 1180
BstC8I GCNNGC 3 cut(s) 336, 768, 932
BstF5I GGATG 1 cut(s) 729
BstKTI GATC 3 cut(s) 60, 916, 1426
BstMAI GTCTC 2 cut(s) 230, 882
BstMBI GATC 3 cut(s) 57, 913, 1423
BstMWI GCNNNNNNNGC 6 cut(s) 187, 206, 680, 1202, 1211, 1405
BstNI CCWGG 1 cut(s) 1226
BstNSI RCATGY 3 cut(s) 350, 770, 836
BstSCI CCNGG 2 cut(s) 200, 1224
BstSFI CTRYAG 4 cut(s) 48, 420, 1099, 1105
BstSLI GKGCMC 1 cut(s) 676
BstV1I GCAGC 7 cut(s) 78, 162, 174, 1113, 1256, 1308, 1420
BstX2I RGATCY 1 cut(s) 57
BstYI RGATCY 1 cut(s) 57
BsuRI GGCC 2 cut(s) 80, 934
BtgZI GCGATG 1 cut(s) 859
BtsCI GGATG 1 cut(s) 729
BtsI GCAGTG 1 cut(s) 468
BtsIMutI CAGTG 2 cut(s) 468, 1118
Cac8I GCNNGC 3 cut(s) 336, 768, 932
Cfr10I RCCGGY 1 cut(s) 257
Cfr13I GGNCC 2 cut(s) 44, 280
Csp6I GTAC 7 cut(s) 436, 661, 668, 715, 1110, 1123, 1181
CviAII CATG 9 cut(s) 347, 485, 664, 767, 833, 854, 1036, 1382, 1427
CviQI GTAC 7 cut(s) 436, 661, 668, 715, 1110, 1123, 1181
DpnI GATC 3 cut(s) 59, 915, 1425
DpnII GATC 3 cut(s) 57, 913, 1423
DriI GACNNNNNGTC 1 cut(s) 210
EaeI YGGCCR 1 cut(s) 78
Eam1104I CTCTTC 2 cut(s) 102, 1314
Eam1105I GACNNNNNGTC 1 cut(s) 210
EarI CTCTTC 2 cut(s) 102, 1314
Eco130I CCWWGG 1 cut(s) 40
Eco147I AGGCCT 1 cut(s) 934
Eco31I GGTCTC 1 cut(s) 230
Eco47I GGWCC 2 cut(s) 44, 280
Eco57I CTGAAG 1 cut(s) 891
Eco88I CYCGRG 1 cut(s) 677
EcoO109I RGGNCCY 2 cut(s) 44, 280
EcoRII CCWGG 1 cut(s) 1224
EcoT14I CCWWGG 1 cut(s) 40
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 9 cut(s) 350, 488, 667, 770, 836, 857, 1039, 1385, 1430
FalI AAGNNNNNCTT 2 cut(s) 1176, 1208
FaqI GGGAC 3 cut(s) 217, 266, 1203
FatI CATG 9 cut(s) 346, 484, 663, 766, 832, 853, 1035, 1381, 1426
FauI CCCGC 2 cut(s) 1378, 1423
FbaI TGATCA 1 cut(s) 1423
Fnu4HI GCNGC 8 cut(s) 92, 151, 188, 1102, 1230, 1245, 1297, 1409
FokI GGATG 1 cut(s) 736
Fsp4HI GCNGC 8 cut(s) 92, 151, 188, 1102, 1230, 1245, 1297, 1409
FspBI CTAG 4 cut(s) 162, 335, 608, 1278
GluI GCNGC 8 cut(s) 92, 151, 188, 1102, 1230, 1245, 1297, 1409
GsaI CCCAGC 1 cut(s) 1200
HaeIII GGCC 2 cut(s) 80, 934
HapII CCGG 3 cut(s) 201, 249, 258
Hin1II CATG 9 cut(s) 350, 488, 667, 770, 836, 857, 1039, 1385, 1430
HincII GTYRAC 2 cut(s) 120, 126
HindII GTYRAC 2 cut(s) 120, 126
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 8 cut(s) 110, 116, 230, 416, 523, 696, 1012, 1028
HpaII CCGG 3 cut(s) 201, 249, 258
Hpy166II GTNNAC 3 cut(s) 120, 126, 468
Hpy188I TCNGA 6 cut(s) 115, 235, 271, 518, 910, 1079
Hpy188III TCNNGA 7 cut(s) 142, 443, 619, 691, 721, 778, 1003
Hpy8I GTNNAC 3 cut(s) 120, 126, 468
Hpy99I CGWCG 2 cut(s) 216, 1175
HpyAV CCTTC 5 cut(s) 227, 271, 878, 915, 1234
HpyCH4III ACNGT 3 cut(s) 34, 1109, 1122
HpyCH4IV ACGT 6 cut(s) 122, 394, 670, 1056, 1112, 1179
HpyF10VI GCNNNNNNNGC 6 cut(s) 187, 206, 680, 1202, 1211, 1405
HpySE526I ACGT 6 cut(s) 122, 394, 670, 1056, 1112, 1179
Hsp92II CATG 9 cut(s) 350, 488, 667, 770, 836, 857, 1039, 1385, 1430
KpnI GGTACC 1 cut(s) 718
Ksp22I TGATCA 1 cut(s) 1423
Kzo9I GATC 3 cut(s) 57, 913, 1423
LmnI GCTCC 2 cut(s) 829, 1079
Lsp1109I GCAGC 7 cut(s) 78, 162, 174, 1113, 1256, 1308, 1420
LweI GCATC 2 cut(s) 190, 316
MaeI CTAG 4 cut(s) 162, 335, 608, 1278
MaeII ACGT 6 cut(s) 122, 394, 670, 1056, 1112, 1179
MaeIII GTNAC 5 cut(s) 164, 577, 1052, 1132, 1256
MalI GATC 3 cut(s) 59, 915, 1425
MboI GATC 3 cut(s) 57, 913, 1423
MboII GAAGA 7 cut(s) 116, 119, 148, 425, 458, 991, 1331
MfeI CAATTG 1 cut(s) 1128
MflI RGATCY 1 cut(s) 57
MhlI GDGCHC 2 cut(s) 364, 676
MlsI TGGCCA 1 cut(s) 80
MluNI TGGCCA 1 cut(s) 80
MlyI GAGTC 4 cut(s) 119, 125, 224, 705
MmeI TCCRAC 4 cut(s) 93, 682, 807, 1362
Mox20I TGGCCA 1 cut(s) 80
MscI TGGCCA 1 cut(s) 80
MseI TTAA 3 cut(s) 387, 648, 1155
MslI CAYNNNNRTG 2 cut(s) 329, 1139
Msp20I TGGCCA 1 cut(s) 80
MspI CCGG 3 cut(s) 201, 249, 258
MspR9I CCNGG 2 cut(s) 202, 1226
MunI CAATTG 1 cut(s) 1128
Mva1269I GAATGC 1 cut(s) 785
MvaI CCWGG 1 cut(s) 1226
MwoI GCNNNNNNNGC 6 cut(s) 187, 206, 680, 1202, 1211, 1405
NciI CCSGG 1 cut(s) 202
NdeII GATC 3 cut(s) 57, 913, 1423
NheI GCTAGC 1 cut(s) 334
NlaIII CATG 9 cut(s) 350, 488, 667, 770, 836, 857, 1039, 1385, 1430
NlaIV GGNNCC 6 cut(s) 253, 262, 282, 716, 820, 926
NmeAIII GCCGAG 2 cut(s) 157, 889
NmuCI GTSAC 2 cut(s) 577, 1132
NspI RCATGY 3 cut(s) 350, 770, 836
PaeI GCATGC 1 cut(s) 770
PaeR7I CTCGAG 1 cut(s) 677
PceI AGGCCT 1 cut(s) 934
PcsI WCGNNNNNNNCGW 3 cut(s) 119, 1167, 1176
PctI GAATGC 1 cut(s) 785
PfeI GAWTC 4 cut(s) 416, 523, 1012, 1028
PflMI CCANNNNNTGG 1 cut(s) 87
PkrI GCNGC 8 cut(s) 93, 152, 189, 1103, 1231, 1246, 1298, 1410
PleI GAGTC 4 cut(s) 118, 124, 224, 704
PpsI GAGTC 4 cut(s) 118, 124, 224, 704
Ppu21I YACGTR 2 cut(s) 671, 1180
PpuMI RGGWCCY 2 cut(s) 44, 280
PsiI TTATAA 1 cut(s) 839
Psp5II RGGWCCY 2 cut(s) 44, 280
Psp6I CCWGG 1 cut(s) 1224
PspFI CCCAGC 1 cut(s) 1196
PspGI CCWGG 1 cut(s) 1224
PspN4I GGNNCC 6 cut(s) 253, 262, 282, 716, 820, 926
PspPI GGNCC 2 cut(s) 44, 280
PspPPI RGGWCCY 2 cut(s) 44, 280
PspXI VCTCGAGB 1 cut(s) 677
PsrI GAACNNNNNNTAC 2 cut(s) 1258, 1290
PstI CTGCAG 1 cut(s) 1103
PsuI RGATCY 1 cut(s) 57
RsaI GTAC 7 cut(s) 437, 662, 669, 716, 1111, 1124, 1182
RsaNI GTAC 7 cut(s) 436, 661, 668, 715, 1110, 1123, 1181
RseI CAYNNNNRTG 2 cut(s) 329, 1139
SaqAI TTAA 3 cut(s) 387, 648, 1155
SatI GCNGC 8 cut(s) 92, 151, 188, 1102, 1230, 1245, 1297, 1409
Sau3AI GATC 3 cut(s) 57, 913, 1423
Sau96I GGNCC 2 cut(s) 44, 280
SchI GAGTC 4 cut(s) 119, 125, 224, 705
ScrFI CCNGG 2 cut(s) 202, 1226
SduI GDGCHC 2 cut(s) 364, 676
SfaNI GCATC 2 cut(s) 190, 316
SfcI CTRYAG 4 cut(s) 48, 420, 1099, 1105
Sfr274I CTCGAG 1 cut(s) 677
SinI GGWCC 2 cut(s) 44, 280
SlaI CTCGAG 1 cut(s) 677
SmiMI CAYNNNNRTG 2 cut(s) 329, 1139
SmlI CTYRAG 2 cut(s) 677, 1368
SmoI CTYRAG 2 cut(s) 677, 1368
SpeI ACTAGT 1 cut(s) 161
SphI GCATGC 1 cut(s) 770
SseBI AGGCCT 1 cut(s) 934
SsiI CCGC 4 cut(s) 928, 1229, 1385, 1416
SspI AATATT 1 cut(s) 1167
SspMI CTAG 4 cut(s) 162, 335, 608, 1278
StuI AGGCCT 1 cut(s) 934
StyD4I CCNGG 2 cut(s) 200, 1224
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 3 cut(s) 34, 1109, 1122
TaiI ACGT 6 cut(s) 125, 397, 673, 1059, 1115, 1182
TaqI TCGA 3 cut(s) 678, 937, 1170
TatI WGTACW 2 cut(s) 435, 660
TauI GCSGC 1 cut(s) 1232
TfiI GAWTC 4 cut(s) 416, 523, 1012, 1028
Tru1I TTAA 3 cut(s) 387, 648, 1155
Tru9I TTAA 3 cut(s) 387, 648, 1155
TscAI CASTG 2 cut(s) 475, 1125
TseFI GTSAC 2 cut(s) 577, 1132
TseI GCWGC 7 cut(s) 91, 150, 187, 1101, 1244, 1296, 1408
Tsp45I GTSAC 2 cut(s) 577, 1132
TspDTI ATGAA 7 cut(s) 117, 313, 501, 604, 787, 947, 1370
TspGWI ACGGA 2 cut(s) 783, 1366
TspRI CASTG 2 cut(s) 475, 1125
Van91I CCANNNNNTGG 1 cut(s) 87
VpaK11BI GGWCC 2 cut(s) 44, 280
XapI RAATTY 5 cut(s) 488, 643, 976, 1022, 1392
XceI RCATGY 3 cut(s) 350, 770, 836
XhoI CTCGAG 1 cut(s) 677
XspI CTAG 4 cut(s) 162, 335, 608, 1278
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.