FvH4_3g16430

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0014625
Physical Location & Seq
Reverse (-)
10338574 .. 10353553
14980 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g16430.t1

Sequence Viewer

Length: 1575 bp
ATGGCAGCTGGAGGGACAACGTTGGAGCAAACACCGACATGGGCGGTGGCTTTTGTGTGTTCTGTTATAGTCTTCATTTCTTTAGTCGTCGAGAAATTACTTCACTGCACTGGCAAGTATCTCAAGAGAAAGAAGCAGATACCTCTCTTCGAGGCCTTGCAGAAGATCAAAGAAGAGTTGATGCTTTTGGGGTTTATATCACTCCTGCTGACGGTGTTCCAAGATTCTATAGAAAAGTTCTGCATATCAAAGAAACTTGCCAGTCATTGGCTGCCTTGTAAGGAAGAAGAGCCGAAGTCAAAGTCAACGACTGGCCATTTCCAGACCAGCAGCTTCTTCACTAGTAACCATTATTCTCGGCATCTTCTTGCAGCCAAGAACCCTAAATCCTGTTCAGAGCAGGGGAAGGTCCCATTGCTATCCACAACTGCATTGCATCATCTACATATTTTCATATTTGTGCTAGCTCTGGCGCATGTGACTTTCTGTGCTCTAACCATTATTTTTGGAGGAATTAAGATGCGTCAATGGAAACAATGGGAAGATTCTATAGAAAGAAAAGAGTACAATCCAGAAGAAGTTTTGACAGAGAAGGACTTCACTGCCGTTCAAGACCATGATTTTATCAGAGGTCGTTTTCTGGGTATTGGAAAGAATCAAGCTTTTATGGGTTGGTTGATTGCATTTTTCAAGCAATTTTATGGGTCTGTGACCAAAACAGATTACATGACAATGCGACTAGGCTTCATCAAGACACATTGCAAGGGAAATCCAAATTTTAATTTCCACACGTACATGGTACGTGCCCTCGAAGCAGATTTCAAGAAAGTCATCAGAATAAGTTGGTACCTCTGGATATTTGTAGTCCTTTTCTTGTTGCTGAATGTTGCTGGTTGGCATATCTATTTCTGGACATCATTCGTTCCGTTCTTTCTTTTACTTGCTGTTGGCACCAAGTTGGAGCATGTTATAATCCAGTTAGCCCATGATGTTGCCGATAAGCACATGGCAATAGAAGGAGACTTGGTGGTTCGACCTTCAGACGATCACTTCTGGTTGCGCAGGCCTCGATTGGTTCTCTTACTCATTCATATCATCTTGTTCCAAAATTCTTTTGAACTCGCATTCTGCTTCTGGATATGGGTTCAATATAATTTTGATTCCTGCATGATGGGGAAAATTGGTTACGTTATCCCAAGACTTGTGATCGGAGTGTTTGTCCAGTTCGTCTGCAGCTACAGTACGTTGCCACTGTACGCAATTGTCACACAGATGGGAACATCTTTTAAGAAGGCAATATTCGACGAACACGTACAAGAGGGACTTGTTGGGTGGGCTAAATCGGCAAGAAAACACCAGGCGGATTTGAGGAAGGCAGCTACTGATGGCGGCGATGGCAGTAGTAGTGGTTCTAGCAAAGTCAGACATGGCAGCAAATATTACAGAATGCACAAAGAAGAGGCAACTTCTGTGTCAAACACCACATCCGGGAAACTTGAATTGGCTCAAGTACTTCCCGTTCATGAGGGAGCAATCCAGCCTCATCAACAGCCACCCACCAATGATCATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

525

Amino Acids

59.93

Weight (kDa)

9.13

Isoelectric Point (pI)

34.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 470 1.2e-190 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014625)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g00060 FvH4_1g00060 FvH4_1g00060 FvH4_3g16430
malus_domestica MD02G1001300.v1.1
prunus_persica Prupe.7G271400_v2.0.a1 Prupe.7G271400_v2.0.a1
pyrus_communis pycom290g00060
rosa_chinensis RchiOBHm_Chr2g0084281
rosa_laevigata RLG00000015606
rosa_multiflora Rmu_sc0003129.1_g000012
rosa_rugosa Rorug01G0452400
rosa_samantha Rh2AG000800 Rh2BG000700 Rh2CG000600 Rh2DG000800
rosa_wichuraiana Rw2G000060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 971
Acc16I TGCGCA 1 cut(s) 1061
Acc65I GGTACC 1 cut(s) 846
AccB1I GGYRCC 2 cut(s) 846, 950
AccB7I CCANNNNNTGG 1 cut(s) 267
AciI CCGC 3 cut(s) 44, 1361, 1389
AclI AACGTT 1 cut(s) 20
AcoI YGGCCR 1 cut(s) 313
AcsI RAATTY 2 cut(s) 775, 1108
AcuI CTGAAG 1 cut(s) 1023
AfaI GTAC 8 cut(s) 566, 794, 801, 848, 1243, 1256, 1314, 1512
AfiI CCNNNNNNNGG 3 cut(s) 211, 267, 1488
AflIII ACRYGT 2 cut(s) 789, 1309
AgsI TTSAA 6 cut(s) 611, 691, 823, 1118, 1148, 1499
AhlI ACTAGT 1 cut(s) 341
AjnI CCWGG 1 cut(s) 1356
AjuI GAANNNNNNNTTGG 2 cut(s) 1484, 1516
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
AluBI AGCT 6 cut(s) 8, 333, 467, 662, 1236, 1379
AluI AGCT 6 cut(s) 8, 333, 467, 662, 1236, 1379
Alw21I GWGCWC 1 cut(s) 493
Alw26I GTCTC 1 cut(s) 1014
AlwNI CAGNNNCTG 1 cut(s) 1382
AoxI GGCC 3 cut(s) 153, 313, 1064
ApeKI GCWGC 7 cut(s) 5, 271, 330, 371, 1233, 1376, 1431
ApoI RAATTY 2 cut(s) 775, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 287, 319
Asp700I GAANNNNTTC 1 cut(s) 596
Asp718I GGTACC 1 cut(s) 846
AspLEI GCGC 2 cut(s) 475, 1062
AspS9I GGNCC 1 cut(s) 409
AsuC2I CCSGG 1 cut(s) 1489
AsuNHI GCTAGC 1 cut(s) 463
AvaII GGWCC 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 808
BalI TGGCCA 1 cut(s) 315
BanI GGYRCC 2 cut(s) 846, 950
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 1 cut(s) 493
BbvI GCAGC 7 cut(s) 17, 258, 342, 383, 1245, 1388, 1443
BccI CCATC 4 cut(s) 1165, 1267, 1379, 1388
BceAI ACGGC 1 cut(s) 590
BciT130I CCWGG 1 cut(s) 1358
BclI TGATCA 1 cut(s) 1564
BcnI CCSGG 1 cut(s) 1489
BcoDI GTCTC 1 cut(s) 1014
BcuI ACTAGT 1 cut(s) 341
BfaI CTAG 4 cut(s) 342, 464, 740, 1413
BfmI CTRYAG 4 cut(s) 228, 549, 1231, 1237
BisI GCNGC 8 cut(s) 6, 272, 331, 372, 1234, 1377, 1390, 1432
BlsI GCNGC 8 cut(s) 7, 273, 332, 373, 1235, 1378, 1391, 1433
BmcAI AGTACT 1 cut(s) 1512
Bme1390I CCNGG 2 cut(s) 1358, 1489
Bme18I GGWCC 1 cut(s) 409
BmgT120I GGNCC 1 cut(s) 409
BmiI GGNNCC 3 cut(s) 411, 848, 952
BmrFI CCNGG 2 cut(s) 1358, 1489
BmsI GCATC 4 cut(s) 171, 370, 445, 510
BmtI GCTAGC 1 cut(s) 467
BpiI GAAGAC 1 cut(s) 64
BpmI CTGGAG 1 cut(s) 30
BpuEI CTTGAG 2 cut(s) 107, 1491
BpuMI CCSGG 1 cut(s) 1489
BsaAI YACGTR 3 cut(s) 792, 803, 1312
BsaXI ACNNNNNCTCC 4 cut(s) 1011, 1041, 1203, 1233
Bsc4I CCNNNNNNNGG 3 cut(s) 211, 267, 1488
Bse1I ACTGG 5 cut(s) 115, 261, 316, 976, 1222
Bse3DI GCAATG 3 cut(s) 413, 431, 757
BseBI CCWGG 1 cut(s) 1358
BseGI GGATG 1 cut(s) 1484
BseLI CCNNNNNNNGG 3 cut(s) 211, 267, 1488
BseMI GCAATG 3 cut(s) 413, 431, 757
BseNI ACTGG 5 cut(s) 115, 261, 316, 976, 1222
BseSI GKGCMC 1 cut(s) 808
BseXI GCAGC 7 cut(s) 17, 258, 342, 383, 1245, 1388, 1443
BsgI GTGCAG 1 cut(s) 91
BshFI GGCC 3 cut(s) 155, 315, 1066
BshNI GGYRCC 2 cut(s) 846, 950
BsiHKAI GWGCWC 1 cut(s) 493
BsiSI CCGG 1 cut(s) 1488
BslFI GGGAC 3 cut(s) 28, 395, 1335
BslI CCNNNNNNNGG 3 cut(s) 211, 267, 1488
BsmAI GTCTC 1 cut(s) 1014
BsmFI GGGAC 3 cut(s) 28, 395, 1335
BsmI GAATGC 2 cut(s) 1124, 1452
BsnI GGCC 3 cut(s) 155, 315, 1066
Bsp1286I GDGCHC 2 cut(s) 493, 808
Bsp143I GATC 4 cut(s) 165, 1045, 1206, 1564
BspACI CCGC 3 cut(s) 44, 1361, 1389
BspANI GGCC 3 cut(s) 155, 315, 1066
BspHI TCATGA 1 cut(s) 1522
BspLI GGNNCC 3 cut(s) 411, 848, 952
BspMAI CTGCAG 1 cut(s) 1235
BspOI GCTAGC 1 cut(s) 467
BspQI GCTCTTC 1 cut(s) 282
BspT107I GGYRCC 2 cut(s) 846, 950
BsrDI GCAATG 3 cut(s) 413, 431, 757
BsrI ACTGG 5 cut(s) 115, 261, 316, 976, 1222
BssMI GATC 4 cut(s) 165, 1045, 1206, 1564
Bst2UI CCWGG 1 cut(s) 1358
Bst4CI ACNGT 3 cut(s) 214, 1241, 1254
Bst6I CTCTTC 4 cut(s) 152, 168, 282, 1452
BstBAI YACGTR 3 cut(s) 792, 803, 1312
BstC8I GCNNGC 2 cut(s) 465, 1064
BstF5I GGATG 1 cut(s) 1484
BstHHI GCGC 2 cut(s) 475, 1062
BstKTI GATC 4 cut(s) 168, 1048, 1209, 1567
BstMAI GTCTC 1 cut(s) 1014
BstMBI GATC 4 cut(s) 165, 1045, 1206, 1564
BstMWI GCNNNNNNNGC 3 cut(s) 812, 1343, 1395
BstNI CCWGG 1 cut(s) 1358
BstNSI RCATGY 2 cut(s) 479, 968
BstSCI CCNGG 2 cut(s) 1356, 1487
BstSFI CTRYAG 4 cut(s) 228, 549, 1231, 1237
BstSLI GKGCMC 1 cut(s) 808
BstV1I GCAGC 7 cut(s) 17, 258, 342, 383, 1245, 1388, 1443
BstV2I GAAGAC 1 cut(s) 64
BsuRI GGCC 3 cut(s) 155, 315, 1066
BtgZI GCGATG 1 cut(s) 1407
BtsCI GGATG 1 cut(s) 1484
BtsI GCAGTG 2 cut(s) 103, 600
BtsIMutI CAGTG 4 cut(s) 103, 108, 600, 1250
Cac8I GCNNGC 2 cut(s) 465, 1064
CaiI CAGNNNCTG 1 cut(s) 1382
CciI TCATGA 1 cut(s) 1522
CfoI GCGC 2 cut(s) 475, 1062
Cfr13I GGNCC 1 cut(s) 409
CseI GACGC 1 cut(s) 512
Csp6I GTAC 8 cut(s) 565, 793, 800, 847, 1242, 1255, 1313, 1511
CviQI GTAC 8 cut(s) 565, 793, 800, 847, 1242, 1255, 1313, 1511
DpnI GATC 4 cut(s) 167, 1047, 1208, 1566
DpnII GATC 4 cut(s) 165, 1045, 1206, 1564
EaeI YGGCCR 1 cut(s) 313
Eam1104I CTCTTC 4 cut(s) 152, 168, 282, 1452
EarI CTCTTC 4 cut(s) 152, 168, 282, 1452
EciI GGCGGA 1 cut(s) 1376
Eco147I AGGCCT 2 cut(s) 155, 1066
Eco47I GGWCC 1 cut(s) 409
Eco57I CTGAAG 1 cut(s) 1023
EcoO109I RGGNCCY 1 cut(s) 409
EcoRII CCWGG 1 cut(s) 1356
EcoT22I ATGCAT 1 cut(s) 1573
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 3 cut(s) 28, 395, 1335
FbaI TGATCA 1 cut(s) 1564
Fnu4HI GCNGC 8 cut(s) 6, 272, 331, 372, 1234, 1377, 1390, 1432
FokI GGATG 1 cut(s) 1471
Fsp4HI GCNGC 8 cut(s) 6, 272, 331, 372, 1234, 1377, 1390, 1432
FspBI CTAG 4 cut(s) 342, 464, 740, 1413
FspI TGCGCA 1 cut(s) 1061
GlaI GCGC 2 cut(s) 474, 1061
GluI GCNGC 8 cut(s) 6, 272, 331, 372, 1234, 1377, 1390, 1432
GsuI CTGGAG 1 cut(s) 30
HaeIII GGCC 3 cut(s) 155, 315, 1066
HapII CCGG 1 cut(s) 1488
HgaI GACGC 1 cut(s) 512
HhaI GCGC 2 cut(s) 475, 1062
Hin6I GCGC 2 cut(s) 473, 1060
HinP1I GCGC 2 cut(s) 473, 1060
HincII GTYRAC 1 cut(s) 306
HindII GTYRAC 1 cut(s) 306
HindIII AAGCTT 1 cut(s) 660
HinfI GANTC 4 cut(s) 224, 545, 655, 1160
HpaII CCGG 1 cut(s) 1488
Hpy166II GTNNAC 1 cut(s) 306
Hpy188I TCNGA 6 cut(s) 397, 629, 836, 1042, 1211, 1424
Hpy8I GTNNAC 1 cut(s) 306
Hpy99I CGWCG 2 cut(s) 92, 1307
HpyAV CCTTC 6 cut(s) 400, 586, 1010, 1047, 1285, 1366
HpyCH4III ACNGT 3 cut(s) 214, 1241, 1254
HpyCH4IV ACGT 6 cut(s) 20, 791, 802, 1188, 1244, 1311
HpyF10VI GCNNNNNNNGC 3 cut(s) 812, 1343, 1395
HpySE526I ACGT 6 cut(s) 20, 791, 802, 1188, 1244, 1311
HspAI GCGC 2 cut(s) 473, 1060
KpnI GGTACC 1 cut(s) 850
Ksp22I TGATCA 1 cut(s) 1564
Kzo9I GATC 4 cut(s) 165, 1045, 1206, 1564
LguI GCTCTTC 1 cut(s) 282
LmnI GCTCC 3 cut(s) 25, 961, 1529
Lsp1109I GCAGC 7 cut(s) 17, 258, 342, 383, 1245, 1388, 1443
LweI GCATC 4 cut(s) 171, 370, 445, 510
MaeI CTAG 4 cut(s) 342, 464, 740, 1413
MaeII ACGT 6 cut(s) 20, 791, 802, 1188, 1244, 1311
MaeIII GTNAC 5 cut(s) 344, 478, 709, 1184, 1264
MalI GATC 4 cut(s) 167, 1047, 1208, 1566
MboI GATC 4 cut(s) 165, 1045, 1206, 1564
MfeI CAATTG 1 cut(s) 1260
MhlI GDGCHC 2 cut(s) 493, 808
MlsI TGGCCA 1 cut(s) 315
MluNI TGGCCA 1 cut(s) 315
MmeI TCCRAC 1 cut(s) 939
Mox20I TGGCCA 1 cut(s) 315
Mph1103I ATGCAT 1 cut(s) 1573
MroXI GAANNNNTTC 1 cut(s) 596
MscI TGGCCA 1 cut(s) 315
MseI TTAA 3 cut(s) 516, 780, 1287
MslI CAYNNNNRTG 5 cut(s) 37, 458, 731, 794, 1271
Msp20I TGGCCA 1 cut(s) 315
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 1 cut(s) 1488
MspR9I CCNGG 2 cut(s) 1358, 1489
MunI CAATTG 1 cut(s) 1260
Mva1269I GAATGC 2 cut(s) 1124, 1452
MvaI CCWGG 1 cut(s) 1358
MwoI GCNNNNNNNGC 3 cut(s) 812, 1343, 1395
NciI CCSGG 1 cut(s) 1489
NdeII GATC 4 cut(s) 165, 1045, 1206, 1564
NheI GCTAGC 1 cut(s) 463
NlaIV GGNNCC 3 cut(s) 411, 848, 952
NmeAIII GCCGAG 1 cut(s) 337
NmuCI GTSAC 3 cut(s) 478, 709, 1264
NsbI TGCGCA 1 cut(s) 1061
NsiI ATGCAT 1 cut(s) 1573
NspI RCATGY 2 cut(s) 479, 968
PagI TCATGA 1 cut(s) 1522
PceI AGGCCT 2 cut(s) 155, 1066
PciSI GCTCTTC 1 cut(s) 282
PcsI WCGNNNNNNNCGW 1 cut(s) 1308
PctI GAATGC 2 cut(s) 1124, 1452
PdmI GAANNNNTTC 1 cut(s) 596
PfeI GAWTC 4 cut(s) 224, 545, 655, 1160
PflMI CCANNNNNTGG 1 cut(s) 267
PfoI TCCNGGA 1 cut(s) 1487
PkrI GCNGC 8 cut(s) 7, 273, 332, 373, 1235, 1378, 1391, 1433
Ppu21I YACGTR 3 cut(s) 792, 803, 1312
PpuMI RGGWCCY 1 cut(s) 409
PsiI TTATAA 1 cut(s) 971
Psp1406I AACGTT 1 cut(s) 20
Psp5II RGGWCCY 1 cut(s) 409
Psp6I CCWGG 1 cut(s) 1356
PspGI CCWGG 1 cut(s) 1356
PspN4I GGNNCC 3 cut(s) 411, 848, 952
PspPI GGNCC 1 cut(s) 409
PspPPI RGGWCCY 1 cut(s) 409
PsrI GAACNNNNNNTAC 4 cut(s) 1393, 1425, 1503, 1535
PstI CTGCAG 1 cut(s) 1235
PstNI CAGNNNCTG 1 cut(s) 1382
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 8 cut(s) 566, 794, 801, 848, 1243, 1256, 1314, 1512
RsaNI GTAC 8 cut(s) 565, 793, 800, 847, 1242, 1255, 1313, 1511
RseI CAYNNNNRTG 5 cut(s) 37, 458, 731, 794, 1271
SapI GCTCTTC 1 cut(s) 282
SaqAI TTAA 3 cut(s) 516, 780, 1287
SatI GCNGC 8 cut(s) 6, 272, 331, 372, 1234, 1377, 1390, 1432
Sau3AI GATC 4 cut(s) 165, 1045, 1206, 1564
Sau96I GGNCC 1 cut(s) 409
ScaI AGTACT 1 cut(s) 1512
ScrFI CCNGG 2 cut(s) 1358, 1489
SduI GDGCHC 2 cut(s) 493, 808
SfaNI GCATC 4 cut(s) 171, 370, 445, 510
SfcI CTRYAG 4 cut(s) 228, 549, 1231, 1237
SinI GGWCC 1 cut(s) 409
SmiMI CAYNNNNRTG 5 cut(s) 37, 458, 731, 794, 1271
SmlI CTYRAG 2 cut(s) 122, 1506
SmoI CTYRAG 2 cut(s) 122, 1506
SpeI ACTAGT 1 cut(s) 341
SseBI AGGCCT 2 cut(s) 155, 1066
SsiI CCGC 3 cut(s) 44, 1361, 1389
SspI AATATT 2 cut(s) 1299, 1439
SspMI CTAG 4 cut(s) 342, 464, 740, 1413
StuI AGGCCT 2 cut(s) 155, 1066
StyD4I CCNGG 2 cut(s) 1356, 1487
TaaI ACNGT 3 cut(s) 214, 1241, 1254
TaiI ACGT 6 cut(s) 23, 794, 805, 1191, 1247, 1314
TaqI TCGA 6 cut(s) 90, 150, 810, 1033, 1069, 1302
TatI WGTACW 2 cut(s) 564, 1510
TauI GCSGC 1 cut(s) 1392
TfiI GAWTC 4 cut(s) 224, 545, 655, 1160
Tru1I TTAA 3 cut(s) 516, 780, 1287
Tru9I TTAA 3 cut(s) 516, 780, 1287
TscAI CASTG 4 cut(s) 110, 115, 607, 1257
TseFI GTSAC 3 cut(s) 478, 709, 1264
TseI GCWGC 7 cut(s) 5, 271, 330, 371, 1233, 1376, 1431
Tsp45I GTSAC 3 cut(s) 478, 709, 1264
TspDTI ATGAA 5 cut(s) 64, 442, 736, 1079, 1511
TspGWI ACGGA 1 cut(s) 915
TspRI CASTG 4 cut(s) 110, 115, 607, 1257
Van91I CCANNNNNTGG 1 cut(s) 267
VpaK11BI GGWCC 1 cut(s) 409
XapI RAATTY 2 cut(s) 775, 1108
XceI RCATGY 2 cut(s) 479, 968
XmnI GAANNNNTTC 1 cut(s) 596
XspI CTAG 4 cut(s) 342, 464, 740, 1413
ZrmI AGTACT 1 cut(s) 1512
Zsp2I ATGCAT 1 cut(s) 1573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.