MD02G1001300.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014625
Physical Location & Seq
Forward (+)
76866 .. 80731
3866 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1001300.v1.1.491

Sequence Viewer

Length: 1623 bp
ATGGCAGAAGGAGGAACAACCTTGGAGTACACTCCGACTTGGGTGGTGGCTTTGGTGTCTAGTGTCATAGTCATCCTATCACTTTTCCTCGAGAGAACCCTTCACTACACTGGCAAGTATCTCAAGAAACACAACCAGAAACCTCTCTTTCAGGCCTTGCAGAAGATCAAAGAAGAGTTGATGCTTTTGGGGTTTATATCACTGCTGCTGACGGTGTTCCAAGGACGTATAGCCAAAATCTGTATAACCGAAAAACAGGCCGGTCAATGGCTGCCTTGCAAGAAAGAGACCACCAGTACTAGTACCAGTGCCCATTTCCAGACCGTGTTCTTTCCCTGGAGCAGTGCTGCCCGTCGTCTTCTTGCTGAAGACTCTGCTTCCACCACGTCCGGCTCCTGTTCAGAGGGGAAGGTCCCTCTGTTATCCACCACGGCATTGCATCATCTTCACATCTTCATTTTTGTGCTGGCTGTGGTGCATGTGACTTTCTGTGTTCTAACCATTCTTTTTGGAGGAGCAAAGATACGTCAATGGAAACTTTGGGAGGATTCTATCACCAAAAAGGAGTACAACCCACAAGAAGTTTTGACAATGAAGTTCACCCACGTCCACGATCATGATTTTATTAGGGGCCGCTTTCGTGGTATTGGAAAGAATTCAGCTATTCTTGGCTGGTTGCATGCATTTTTCAAGCAATTTTATGGCTCTGTGACCAAAACAGATTATATGACAATGCGACTAGGCTTCATCAGCACCCATTGCAAGGCAAATCCAAATTTTAATTTCCACAAGTACATGATTCGTGCCCTTGAAGCAGACTTTAAAAGAGTCGTTGGGATAAGCTGGTATCTTTGGATCTTTGTGGTCATCTTCTTGTTGCTGAATGTGTCGGGTTGGCATGCCTATTTCTGGATAGCATTCATTCCATTCATCCTTTTACTTGCGGTTGGGACCAAGTTGGAGCATGTAATAAGTCAACTGGCCCATGAGGTTGCTGAGAAACACATTGCAATCGAAGGTGACTTGGTTGTTCAGCCTTCAGATGATCATTTTTGGTTCCACCGGCCCAGACTCGTTCTCTTACTCGTTCATATCATCTTGTTCCAAAATTCGTTTGAACTCGCATTTTTCTTCTGGATATTGGTTCAGTATAAATTTCATTCCTGCATGATGGGGGAACTTGGTTATGTTATCCCAAGGCTTGTAATTGGAGTATTCGTTCAGTTCGTCTGCAGTTACAGTACCTTGCCACTTTACGCAATTGTCACACAGATGGGAAGTTCGTTCAAGAAGGCTATATTTGAAGAACATATACAAGAGGGGCTTGTTGGGTGGGCTCGGTCGGCCAAAAAGAATAAGGCGTTGAGAAACGCAGCTAATGCGAATGGTTCTGGTTCTGGTGCTGGTGCTGGTTCTACTCAAGTAGGTGTAGGTGTAGGTCTCAAAGAGAAAGAGAAAGAGGCTTCAGCGACACTTGAATTGGCTCAAAAACTGAAAGTTGGAAACAAGGAGGGATCTTCTCTCCAACGTCATGACACTACTTCTACAGGTGCGGCTGCGGCTGTGACTGCCGGAGAAATTGAGCCCCAACAGCCACTCATACAGCAAAGTAAACTTCAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

541

Amino Acids

60.66

Weight (kDa)

9.35

Isoelectric Point (pI)

32.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 462 4.2e-191 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014625)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g00060 FvH4_1g00060 FvH4_1g00060 FvH4_3g16430
malus_domestica MD02G1001300.v1.1
prunus_persica Prupe.7G271400_v2.0.a1 Prupe.7G271400_v2.0.a1
pyrus_communis pycom290g00060
rosa_chinensis RchiOBHm_Chr2g0084281
rosa_laevigata RLG00000015606
rosa_multiflora Rmu_sc0003129.1_g000012
rosa_rugosa Rorug01G0452400
rosa_samantha Rh2AG000800 Rh2BG000700 Rh2CG000600 Rh2DG000800
rosa_wichuraiana Rw2G000060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 4 cut(s) 634, 944, 1553, 1559
AclWI GGATC 2 cut(s) 863, 1522
AcoI YGGCCR 1 cut(s) 1344
AcsI RAATTY 4 cut(s) 655, 775, 1108, 1154
AcuI CTGAAG 3 cut(s) 387, 1023, 1449
AfaI GTAC 6 cut(s) 29, 298, 304, 569, 794, 1243
AfiI CCNNNNNNNGG 3 cut(s) 267, 763, 909
AgsI TTSAA 7 cut(s) 691, 812, 1118, 1288, 1304, 1478, 1619
AhlI ACTAGT 1 cut(s) 299
AjiI CACGTC 2 cut(s) 387, 607
AjnI CCWGG 1 cut(s) 335
AloI GAACNNNNNNTCC 2 cut(s) 1203, 1235
AluBI AGCT 3 cut(s) 662, 843, 1376
AluI AGCT 3 cut(s) 662, 843, 1376
Alw26I GTCTC 2 cut(s) 281, 1445
AlwI GGATC 2 cut(s) 863, 1522
Ama87I CYCGRG 1 cut(s) 89
AoxI GGCC 6 cut(s) 153, 258, 631, 981, 1064, 1344
ApeKI GCWGC 5 cut(s) 205, 271, 347, 1373, 1556
ApoI RAATTY 4 cut(s) 655, 775, 1108, 1154
Asp700I GAANNNNTTC 1 cut(s) 655
AspS9I GGNCC 5 cut(s) 412, 631, 951, 982, 1065
AsuHPI GGTGA 3 cut(s) 547, 592, 1031
AvaI CYCGRG 1 cut(s) 89
AvaII GGWCC 2 cut(s) 412, 951
BaeGI GKGCMC 2 cut(s) 313, 808
BanII GRGCYC 2 cut(s) 1339, 1587
BarI GAAGNNNNNNTAC 2 cut(s) 1296, 1328
BbsI GAAGAC 2 cut(s) 350, 375
BbvI GCAGC 5 cut(s) 192, 258, 334, 1385, 1543
BccI CCATC 2 cut(s) 1165, 1267
BceAI ACGGC 1 cut(s) 447
BciT130I CCWGG 1 cut(s) 337
BclI TGATCA 1 cut(s) 1045
BcoDI GTCTC 2 cut(s) 281, 1445
BcuI ACTAGT 1 cut(s) 299
BfaI CTAG 3 cut(s) 60, 300, 740
BfmI CTRYAG 2 cut(s) 1231, 1545
BisI GCNGC 8 cut(s) 206, 272, 348, 634, 1374, 1554, 1557, 1560
BlsI GCNGC 8 cut(s) 207, 273, 349, 635, 1375, 1555, 1558, 1561
BmcAI AGTACT 1 cut(s) 298
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 2 cut(s) 412, 951
BmeT110I CYCGRG 1 cut(s) 89
BmgBI CACGTC 2 cut(s) 387, 607
BmgT120I GGNCC 5 cut(s) 412, 631, 951, 982, 1065
BmiI GGNNCC 5 cut(s) 394, 414, 632, 952, 1058
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 2 cut(s) 171, 448
BpiI GAAGAC 2 cut(s) 350, 375
BpmI CTGGAG 1 cut(s) 358
BpuEI CTTGAG 2 cut(s) 107, 1404
BsaI GGTCTC 2 cut(s) 281, 1445
BsaJI CCNNGG 5 cut(s) 21, 220, 335, 429, 1196
BsaXI ACNNNNNCTCC 2 cut(s) 1203, 1233
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 763, 909
Bse118I RCCGGY 2 cut(s) 260, 1062
Bse1I ACTGG 4 cut(s) 115, 294, 306, 984
Bse3DI GCAATG 3 cut(s) 434, 757, 1005
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 5 cut(s) 21, 220, 335, 429, 1196
BseGI GGATG 2 cut(s) 72, 930
BseLI CCNNNNNNNGG 3 cut(s) 267, 763, 909
BseMI GCAATG 3 cut(s) 434, 757, 1005
BseMII CTCAG 1 cut(s) 987
BseNI ACTGG 4 cut(s) 115, 294, 306, 984
BseRI GAGGAG 1 cut(s) 528
BseSI GKGCMC 2 cut(s) 313, 808
BseXI GCAGC 5 cut(s) 192, 258, 334, 1385, 1543
Bsh1285I CGRYCG 1 cut(s) 1343
BshFI GGCC 6 cut(s) 155, 260, 633, 983, 1066, 1346
BsiEI CGRYCG 1 cut(s) 1343
BsiHKCI CYCGRG 1 cut(s) 89
BsiSI CCGG 4 cut(s) 261, 390, 1063, 1572
BslFI GGGAC 2 cut(s) 398, 964
BslI CCNNNNNNNGG 3 cut(s) 267, 763, 909
BsmAI GTCTC 2 cut(s) 281, 1445
BsmFI GGGAC 2 cut(s) 398, 964
BsmI GAATGC 1 cut(s) 917
BsnI GGCC 6 cut(s) 155, 260, 633, 983, 1066, 1346
Bso31I GGTCTC 2 cut(s) 281, 1445
BsoBI CYCGRG 1 cut(s) 89
Bsp1286I GDGCHC 4 cut(s) 313, 808, 1339, 1587
Bsp143I GATC 5 cut(s) 165, 613, 855, 1045, 1514
BspACI CCGC 4 cut(s) 634, 944, 1553, 1559
BspANI GGCC 6 cut(s) 155, 260, 633, 983, 1066, 1346
BspCNI CTCAG 1 cut(s) 988
BspHI TCATGA 2 cut(s) 616, 1531
BspLI GGNNCC 5 cut(s) 394, 414, 632, 952, 1058
BspMAI CTGCAG 1 cut(s) 1235
BspPI GGATC 2 cut(s) 863, 1522
BspTNI GGTCTC 2 cut(s) 281, 1445
BsrDI GCAATG 3 cut(s) 434, 757, 1005
BsrFI RCCGGY 2 cut(s) 260, 1062
BsrI ACTGG 4 cut(s) 115, 294, 306, 984
BssAI RCCGGY 2 cut(s) 260, 1062
BssECI CCNNGG 5 cut(s) 21, 220, 335, 429, 1196
BssMI GATC 5 cut(s) 165, 613, 855, 1045, 1514
BssT1I CCWWGG 3 cut(s) 21, 220, 1196
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 3 cut(s) 214, 325, 1241
Bst6I CTCTTC 1 cut(s) 168
BstAPI GCANNNNNTGC 2 cut(s) 759, 1379
BstC8I GCNNGC 3 cut(s) 468, 681, 900
BstDEI CTNAG 1 cut(s) 996
BstDSI CCRYGG 1 cut(s) 429
BstF5I GGATG 2 cut(s) 72, 930
BstKTI GATC 5 cut(s) 168, 616, 858, 1048, 1517
BstMAI GTCTC 2 cut(s) 281, 1445
BstMBI GATC 5 cut(s) 165, 613, 855, 1045, 1514
BstMCI CGRYCG 1 cut(s) 1343
BstMWI GCNNNNNNNGC 8 cut(s) 750, 759, 812, 1343, 1379, 1559, 1568, 1591
BstNI CCWGG 1 cut(s) 337
BstNSI RCATGY 4 cut(s) 482, 683, 902, 968
BstSCI CCNGG 1 cut(s) 335
BstSFI CTRYAG 2 cut(s) 1231, 1545
BstSLI GKGCMC 2 cut(s) 313, 808
BstV1I GCAGC 5 cut(s) 192, 258, 334, 1385, 1543
BstV2I GAAGAC 2 cut(s) 350, 375
BstX2I RGATCY 2 cut(s) 855, 1514
BstYI RGATCY 2 cut(s) 855, 1514
BsuRI GGCC 6 cut(s) 155, 260, 633, 983, 1066, 1346
BtgI CCRYGG 1 cut(s) 429
BtrI CACGTC 2 cut(s) 387, 607
BtsCI GGATG 2 cut(s) 72, 930
BtsI GCAGTG 2 cut(s) 200, 349
BtsIMutI CAGTG 4 cut(s) 108, 200, 313, 349
Cac8I GCNNGC 3 cut(s) 468, 681, 900
CciI TCATGA 2 cut(s) 616, 1531
Cfr10I RCCGGY 2 cut(s) 260, 1062
Cfr13I GGNCC 5 cut(s) 412, 631, 951, 982, 1065
Csp6I GTAC 6 cut(s) 28, 297, 303, 568, 793, 1242
CviAII CATG 9 cut(s) 479, 617, 680, 796, 899, 965, 986, 1168, 1532
CviQI GTAC 6 cut(s) 28, 297, 303, 568, 793, 1242
DdeI CTNAG 1 cut(s) 996
DpnI GATC 5 cut(s) 167, 615, 857, 1047, 1516
DpnII GATC 5 cut(s) 165, 613, 855, 1045, 1514
DraI TTTAAA 1 cut(s) 823
EaeI YGGCCR 1 cut(s) 1344
Eam1104I CTCTTC 1 cut(s) 168
EarI CTCTTC 1 cut(s) 168
Eco130I CCWWGG 3 cut(s) 21, 220, 1196
Eco147I AGGCCT 1 cut(s) 155
Eco24I GRGCYC 2 cut(s) 1339, 1587
Eco31I GGTCTC 2 cut(s) 281, 1445
Eco47I GGWCC 2 cut(s) 412, 951
Eco57I CTGAAG 3 cut(s) 387, 1023, 1449
Eco88I CYCGRG 1 cut(s) 89
EcoO109I RGGNCCY 1 cut(s) 412
EcoRI GAATTC 1 cut(s) 655
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 3 cut(s) 21, 220, 1196
EcoT22I ATGCAT 1 cut(s) 685
EcoT38I GRGCYC 2 cut(s) 1339, 1587
ErhI CCWWGG 3 cut(s) 21, 220, 1196
FaeI CATG 9 cut(s) 482, 620, 683, 799, 902, 968, 989, 1171, 1535
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 2 cut(s) 398, 964
FatI CATG 9 cut(s) 478, 616, 679, 795, 898, 964, 985, 1167, 1531
FbaI TGATCA 1 cut(s) 1045
Fnu4HI GCNGC 8 cut(s) 206, 272, 348, 634, 1374, 1554, 1557, 1560
FokI GGATG 2 cut(s) 59, 917
FriOI GRGCYC 2 cut(s) 1339, 1587
Fsp4HI GCNGC 8 cut(s) 206, 272, 348, 634, 1374, 1554, 1557, 1560
FspBI CTAG 3 cut(s) 60, 300, 740
GluI GCNGC 8 cut(s) 206, 272, 348, 634, 1374, 1554, 1557, 1560
GsuI CTGGAG 1 cut(s) 358
HaeIII GGCC 6 cut(s) 155, 260, 633, 983, 1066, 1346
HapII CCGG 4 cut(s) 261, 390, 1063, 1572
Hin1II CATG 9 cut(s) 482, 620, 683, 799, 902, 968, 989, 1171, 1535
HincII GTYRAC 1 cut(s) 977
HindII GTYRAC 1 cut(s) 977
HinfI GANTC 5 cut(s) 371, 548, 799, 828, 1071
HpaII CCGG 4 cut(s) 261, 390, 1063, 1572
HphI GGTGA 3 cut(s) 547, 592, 1031
Hpy166II GTNNAC 5 cut(s) 30, 600, 610, 977, 1613
Hpy188I TCNGA 3 cut(s) 36, 403, 1042
Hpy188III TCNNGA 8 cut(s) 91, 124, 319, 617, 910, 1135, 1288, 1532
Hpy8I GTNNAC 5 cut(s) 30, 600, 610, 977, 1613
Hpy99I CGWCG 1 cut(s) 357
HpyAV CCTTC 5 cut(s) 110, 403, 1010, 1047, 1285
HpyCH4III ACNGT 3 cut(s) 214, 325, 1241
HpyCH4IV ACGT 5 cut(s) 226, 386, 526, 606, 1528
HpyF10VI GCNNNNNNNGC 8 cut(s) 750, 759, 812, 1343, 1379, 1559, 1568, 1591
HpyF3I CTNAG 1 cut(s) 996
HpySE526I ACGT 5 cut(s) 226, 386, 526, 606, 1528
Hsp92II CATG 9 cut(s) 482, 620, 683, 799, 902, 968, 989, 1171, 1535
Ksp22I TGATCA 1 cut(s) 1045
Kzo9I GATC 5 cut(s) 165, 613, 855, 1045, 1514
LmnI GCTCC 4 cut(s) 339, 398, 515, 961
Lsp1109I GCAGC 5 cut(s) 192, 258, 334, 1385, 1543
LweI GCATC 2 cut(s) 171, 448
MaeI CTAG 3 cut(s) 60, 300, 740
MaeII ACGT 5 cut(s) 226, 386, 526, 606, 1528
MaeIII GTNAC 6 cut(s) 481, 709, 1019, 1235, 1264, 1564
MalI GATC 5 cut(s) 167, 615, 857, 1047, 1516
MboI GATC 5 cut(s) 165, 613, 855, 1045, 1514
MfeI CAATTG 1 cut(s) 1260
MflI RGATCY 2 cut(s) 855, 1514
MhlI GDGCHC 4 cut(s) 313, 808, 1339, 1587
MlyI GAGTC 3 cut(s) 365, 837, 1065
MmeI TCCRAC 4 cut(s) 59, 939, 1480, 1549
Mph1103I ATGCAT 1 cut(s) 685
MroXI GAANNNNTTC 1 cut(s) 655
MseI TTAA 2 cut(s) 780, 822
MslI CAYNNNNRTG 3 cut(s) 461, 615, 1271
MspI CCGG 4 cut(s) 261, 390, 1063, 1572
MspR9I CCNGG 1 cut(s) 337
MunI CAATTG 1 cut(s) 1260
Mva1269I GAATGC 1 cut(s) 917
MvaI CCWGG 1 cut(s) 337
MwoI GCNNNNNNNGC 8 cut(s) 750, 759, 812, 1343, 1379, 1559, 1568, 1591
NdeII GATC 5 cut(s) 165, 613, 855, 1045, 1514
NlaIII CATG 9 cut(s) 482, 620, 683, 799, 902, 968, 989, 1171, 1535
NlaIV GGNNCC 5 cut(s) 394, 414, 632, 952, 1058
NmuCI GTSAC 5 cut(s) 481, 709, 1019, 1264, 1564
NsiI ATGCAT 1 cut(s) 685
NspI RCATGY 4 cut(s) 482, 683, 902, 968
PaeI GCATGC 2 cut(s) 683, 902
PaeR7I CTCGAG 1 cut(s) 89
PagI TCATGA 2 cut(s) 616, 1531
PceI AGGCCT 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 1224
PctI GAATGC 1 cut(s) 917
PdmI GAANNNNTTC 1 cut(s) 655
PfeI GAWTC 2 cut(s) 548, 799
PkrI GCNGC 8 cut(s) 207, 273, 349, 635, 1375, 1555, 1558, 1561
PleI GAGTC 3 cut(s) 365, 836, 1065
PpsI GAGTC 3 cut(s) 365, 836, 1065
PpuMI RGGWCCY 1 cut(s) 412
Psp5II RGGWCCY 1 cut(s) 412
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 5 cut(s) 394, 414, 632, 952, 1058
PspPI GGNCC 5 cut(s) 412, 631, 951, 982, 1065
PspPPI RGGWCCY 1 cut(s) 412
PstI CTGCAG 1 cut(s) 1235
PsuI RGATCY 2 cut(s) 855, 1514
RsaI GTAC 6 cut(s) 29, 298, 304, 569, 794, 1243
RsaNI GTAC 6 cut(s) 28, 297, 303, 568, 793, 1242
RseI CAYNNNNRTG 3 cut(s) 461, 615, 1271
SaqAI TTAA 2 cut(s) 780, 822
SatI GCNGC 8 cut(s) 206, 272, 348, 634, 1374, 1554, 1557, 1560
Sau3AI GATC 5 cut(s) 165, 613, 855, 1045, 1514
Sau96I GGNCC 5 cut(s) 412, 631, 951, 982, 1065
ScaI AGTACT 1 cut(s) 298
SchI GAGTC 3 cut(s) 365, 837, 1065
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 4 cut(s) 313, 808, 1339, 1587
SfaNI GCATC 2 cut(s) 171, 448
SfcI CTRYAG 2 cut(s) 1231, 1545
Sfr274I CTCGAG 1 cut(s) 89
SinI GGWCC 2 cut(s) 412, 951
SlaI CTCGAG 1 cut(s) 89
SmiMI CAYNNNNRTG 3 cut(s) 461, 615, 1271
SmlI CTYRAG 3 cut(s) 89, 122, 1419
SmoI CTYRAG 3 cut(s) 89, 122, 1419
SpeI ACTAGT 1 cut(s) 299
SphI GCATGC 2 cut(s) 683, 902
SseBI AGGCCT 1 cut(s) 155
SsiI CCGC 4 cut(s) 634, 944, 1553, 1559
SspMI CTAG 3 cut(s) 60, 300, 740
StuI AGGCCT 1 cut(s) 155
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 3 cut(s) 21, 220, 1196
TaaI ACNGT 3 cut(s) 214, 325, 1241
TaiI ACGT 5 cut(s) 229, 389, 529, 609, 1531
TaqI TCGA 2 cut(s) 90, 1014
TaqII GACCGA 1 cut(s) 1329
TatI WGTACW 4 cut(s) 27, 296, 567, 792
TauI GCSGC 3 cut(s) 636, 1556, 1562
TfiI GAWTC 2 cut(s) 548, 799
Tru1I TTAA 2 cut(s) 780, 822
Tru9I TTAA 2 cut(s) 780, 822
TscAI CASTG 4 cut(s) 115, 207, 313, 349
TseFI GTSAC 5 cut(s) 481, 709, 1019, 1264, 1564
TseI GCWGC 5 cut(s) 205, 271, 347, 1373, 1556
Tsp45I GTSAC 5 cut(s) 481, 709, 1019, 1264, 1564
TspDTI ATGAA 7 cut(s) 445, 608, 736, 910, 919, 1079, 1148
TspRI CASTG 4 cut(s) 115, 207, 313, 349
VpaK11BI GGWCC 2 cut(s) 412, 951
XapI RAATTY 4 cut(s) 655, 775, 1108, 1154
XceI RCATGY 4 cut(s) 482, 683, 902, 968
XhoI CTCGAG 1 cut(s) 89
XmnI GAANNNNTTC 1 cut(s) 655
XspI CTAG 3 cut(s) 60, 300, 740
ZrmI AGTACT 1 cut(s) 298
Zsp2I ATGCAT 1 cut(s) 685
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.