Rh2DG000800

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014625
Physical Location & Seq
Forward (+)
86932 .. 93650
6719 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG000800.1

Sequence Viewer

Length: 1623 bp
ATGGCCGGAGGAACAACGTTGGAGTACACGCCGACATGGGTGGTGGCTTTTGTGTGCTCCGTTATAGTCTTCATATCTTTAGTAGTCGAGCGATTACTTCACTACACTGGCAAGTATCTCAAGAGAAAGAACCAGAAACCTCTCTTCGAGGCCTTGCAGAAGATCAAAGAAGAGCTGATGCTTTTGGGGTTTATATCACTCCTGCTGACGGTGTTACAAGGACCTATAGAAAAGATCTGCATATCGGAGAAACTGGCCAGTCATTGGCTGCCTTGCAAAGAAGAGTCGAAGTCAACGTCAACGACTGCCCATTTCCAGACCAGCAGCTTCTTCAGTAGTAATTATTATTCTCGGCAGCTGCTGGTTTTTCCAGCCGGGACTGCCCGTCGCCTTCTTGCAGATGACGCTGAGTCGACCTCTGAATCTGGTTCCACCGGCTCATGTCCAGAGGGGAAGGTCCCATTGCTATCCACGACTGCATTGCACCATCTGCATATCTTCATCTTTGTGCTAGCTGTGGTGCATGTGACTTTCTGTGTTCTAACCATTCTTTTTGGAGGAATTAAGATACGTCAATGGAAACAATGGGAAGATTCTATAGTAAGAAAAGAGTACAATCCAGAAGAAGTTTTGACACAGAAGTTCACCGCCGTCCAAGATCATGATTTTATCAGAGGTCGTTTTCTGGGTATCGGAAAGAATCTAGCTTTTCTGGGTTGGTTGATTGCATATTTCAAACAATTTTATGGGTCTGTGACCAAAACAGACTATATGACAATGCGACTAGGCTTCATCAAGACACATTGCAAGGGAAATCCAAATTTTAATTTCCACAAGTACATGGTACGTGCCCTTGAAGCGGATTTCAAGAAAGTCGTTGGAATAAGTTGGTACCTTTGGATCTTTGTGGTCATTTTCTTGTTGCTAAATGTCGCTGGTTGGCATGCCTATTTCTGGATAGCATTCGGTCCTTTCTTTCTTTTACTTGCTGTTGGCACCAAGTTGGAGCATGTTATAATCCAATTAGCCCATGATGTTGCCGAGAAGCACATTGCAATAGAAGGAGACTTGGTGGTTCAACCTTCAGACGATCACTTTTGGTTCCACAGGCCTCGATTGGTTCTCTTACTGATTCATATCATCTTGTTCCAAAATTCTTTTGAACTAGCATTTTTCTTCTGGATATGGATTCAATATTCCTTTAATTCCTGCATGATGGGGAAAATTGGTTACGTTATCCCAAGACTTATAATCGGAGCATTTGTCCAGTTCGTCTGCAGCTACAGTACGTTACCACTCTACGCAATTGTCACACAGATGGGAACAACTTTTAAGAAGGCAATATTCGACGAACACGTACAAGAGGGCCTTGTTGGGTGGGCTAAATCGGCAAAAAAGCACCAGGCGGCGTTAAGAAAGGCGGCTACTAATGGCAGCAGTAGTAATGGTTCTAGCCAAGTAAGTCATAGCAGCAAATACAAATTGCACAGAGAAGAGGCAACTTCTGTGTCCAAGACATCATCGGCGACACTTGAACTGGCTCAAGTTGGAAATAAGGAGTCTTCCGTGCATGTGGGAGAAATTCAGCCTCATCAACTGCCGCCAGCCAATGATCATGTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

540

Amino Acids

61.18

Weight (kDa)

9.08

Isoelectric Point (pI)

30.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 5 - 491 2.5e-192 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014625)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g00060 FvH4_1g00060 FvH4_1g00060 FvH4_3g16430
malus_domestica MD02G1001300.v1.1
prunus_persica Prupe.7G271400_v2.0.a1 Prupe.7G271400_v2.0.a1
pyrus_communis pycom290g00060
rosa_chinensis RchiOBHm_Chr2g0084281
rosa_laevigata RLG00000015606
rosa_multiflora Rmu_sc0003129.1_g000012
rosa_rugosa Rorug01G0452400
rosa_samantha Rh2AG000800 Rh2BG000700 Rh2CG000600 Rh2DG000800
rosa_wichuraiana Rw2G000060

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1016, 1250
Acc65I GGTACC 1 cut(s) 891
AccB1I GGYRCC 2 cut(s) 891, 995
AccB7I CCANNNNNTGG 1 cut(s) 264
AccI GTMKAC 1 cut(s) 413
AciI CCGC 5 cut(s) 648, 860, 1406, 1421, 1601
AclI AACGTT 1 cut(s) 17
AclWI GGATC 1 cut(s) 908
AcoI YGGCCR 2 cut(s) 3, 255
AcsI RAATTY 3 cut(s) 820, 1153, 1581
AcuI CTGAAG 2 cut(s) 316, 1068
AfaI GTAC 7 cut(s) 26, 614, 839, 846, 893, 1288, 1359
AfiI CCNNNNNNNGG 4 cut(s) 208, 264, 859, 954
AflIII ACRYGT 1 cut(s) 1354
AgsI TTSAA 7 cut(s) 736, 857, 868, 1079, 1163, 1193, 1535
AhdI GACNNNNNGTC 2 cut(s) 384, 409
AjnI CCWGG 1 cut(s) 1401
AluBI AGCT 6 cut(s) 175, 327, 358, 515, 707, 1281
AluI AGCT 6 cut(s) 175, 327, 358, 515, 707, 1281
Alw21I GWGCWC 1 cut(s) 59
Alw26I GTCTC 1 cut(s) 1059
AlwI GGATC 1 cut(s) 908
AlwNI CAGNNNCTG 1 cut(s) 361
AoxI GGCC 5 cut(s) 3, 150, 255, 1109, 1366
ApeKI GCWGC 7 cut(s) 268, 324, 355, 358, 1278, 1434, 1470
ApoI RAATTY 3 cut(s) 820, 1153, 1581
ArsI GACNNNNNNTTYG 2 cut(s) 281, 313
Asp718I GGTACC 1 cut(s) 891
AspS9I GGNCC 4 cut(s) 221, 457, 968, 1366
AsuC2I CCSGG 1 cut(s) 376
AsuHPI GGTGA 1 cut(s) 637
AsuNHI GCTAGC 1 cut(s) 511
AvaII GGWCC 3 cut(s) 221, 457, 968
BaeGI GKGCMC 1 cut(s) 853
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 2 cut(s) 891, 995
BbsI GAAGAC 2 cut(s) 61, 1554
Bbv12I GWGCWC 1 cut(s) 59
BbvI GCAGC 7 cut(s) 255, 336, 345, 367, 1290, 1446, 1482
BccI CCATC 3 cut(s) 495, 1210, 1312
BceAI ACGGC 1 cut(s) 635
BcgI CGANNNNNNTGC 2 cut(s) 463, 497
BciT130I CCWGG 1 cut(s) 1403
BclI TGATCA 1 cut(s) 1612
BcnI CCSGG 1 cut(s) 376
BcoDI GTCTC 1 cut(s) 1059
BfaI CTAG 5 cut(s) 512, 704, 785, 1166, 1452
BfmI CTRYAG 4 cut(s) 225, 597, 1276, 1282
BglII AGATCT 1 cut(s) 234
Bme1390I CCNGG 2 cut(s) 376, 1403
Bme18I GGWCC 3 cut(s) 221, 457, 968
BmeRI GACNNNNNGTC 2 cut(s) 384, 409
BmgT120I GGNCC 4 cut(s) 221, 457, 968, 1366
BmiI GGNNCC 5 cut(s) 430, 459, 893, 997, 1103
BmrFI CCNGG 2 cut(s) 376, 1403
BmsI GCATC 1 cut(s) 168
BmtI GCTAGC 1 cut(s) 515
BpiI GAAGAC 2 cut(s) 61, 1554
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpuEI CTTGAG 2 cut(s) 104, 1527
BpuMI CCSGG 1 cut(s) 376
BsaAI YACGTR 2 cut(s) 848, 1357
BsaBI GATNNNNATC 1 cut(s) 1136
BsaXI ACNNNNNCTCC 4 cut(s) 1056, 1086, 1248, 1278
Bsc4I CCNNNNNNNGG 4 cut(s) 208, 264, 859, 954
Bse118I RCCGGY 1 cut(s) 434
Bse1I ACTGG 5 cut(s) 112, 258, 258, 1267, 1542
Bse3DI GCAATG 4 cut(s) 461, 479, 802, 1050
Bse8I GATNNNNATC 1 cut(s) 1136
BseBI CCWGG 1 cut(s) 1403
BseJI GATNNNNATC 1 cut(s) 1136
BseLI CCNNNNNNNGG 4 cut(s) 208, 264, 859, 954
BseMI GCAATG 4 cut(s) 461, 479, 802, 1050
BseMII CTCAG 1 cut(s) 399
BseNI ACTGG 5 cut(s) 112, 258, 258, 1267, 1542
BseSI GKGCMC 1 cut(s) 853
BseXI GCAGC 7 cut(s) 255, 336, 345, 367, 1290, 1446, 1482
BshFI GGCC 5 cut(s) 5, 152, 257, 1111, 1368
BshNI GGYRCC 2 cut(s) 891, 995
BsiHKAI GWGCWC 1 cut(s) 59
BsiSI CCGG 3 cut(s) 6, 375, 435
BslFI GGGAC 2 cut(s) 391, 443
BslI CCNNNNNNNGG 4 cut(s) 208, 264, 859, 954
BsmAI GTCTC 1 cut(s) 1059
BsmFI GGGAC 2 cut(s) 391, 443
BsmI GAATGC 1 cut(s) 962
BsnI GGCC 5 cut(s) 5, 152, 257, 1111, 1368
Bsp1286I GDGCHC 2 cut(s) 59, 853
Bsp143I GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
BspACI CCGC 5 cut(s) 648, 860, 1406, 1421, 1601
BspANI GGCC 5 cut(s) 5, 152, 257, 1111, 1368
BspCNI CTCAG 1 cut(s) 400
BspHI TCATGA 1 cut(s) 661
BspLI GGNNCC 5 cut(s) 430, 459, 893, 997, 1103
BspMAI CTGCAG 1 cut(s) 1280
BspOI GCTAGC 1 cut(s) 515
BspPI GGATC 1 cut(s) 908
BspQI GCTCTTC 1 cut(s) 165
BspT107I GGYRCC 2 cut(s) 891, 995
BsrDI GCAATG 4 cut(s) 461, 479, 802, 1050
BsrFI RCCGGY 1 cut(s) 434
BsrI ACTGG 5 cut(s) 112, 258, 258, 1267, 1542
BssAI RCCGGY 1 cut(s) 434
BssMI GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
Bst2UI CCWGG 1 cut(s) 1403
Bst4CI ACNGT 2 cut(s) 211, 1286
Bst6I CTCTTC 4 cut(s) 149, 165, 276, 1488
BstAPI GCANNNNNTGC 1 cut(s) 490
BstBAI YACGTR 2 cut(s) 848, 1357
BstC8I GCNNGC 3 cut(s) 513, 945, 1605
BstDEI CTNAG 1 cut(s) 408
BstKTI GATC 6 cut(s) 165, 237, 661, 903, 1093, 1615
BstMAI GTCTC 1 cut(s) 1059
BstMBI GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
BstMWI GCNNNNNNNGC 5 cut(s) 380, 404, 490, 857, 1388
BstNI CCWGG 1 cut(s) 1403
BstNSI RCATGY 4 cut(s) 527, 947, 1013, 1574
BstSCI CCNGG 2 cut(s) 374, 1401
BstSFI CTRYAG 4 cut(s) 225, 597, 1276, 1282
BstSLI GKGCMC 1 cut(s) 853
BstV1I GCAGC 7 cut(s) 255, 336, 345, 367, 1290, 1446, 1482
BstV2I GAAGAC 2 cut(s) 61, 1554
BstX2I RGATCY 2 cut(s) 234, 900
BstYI RGATCY 2 cut(s) 234, 900
BsuRI GGCC 5 cut(s) 5, 152, 257, 1111, 1368
BtsIMutI CAGTG 1 cut(s) 105
Cac8I GCNNGC 3 cut(s) 513, 945, 1605
CaiI CAGNNNCTG 1 cut(s) 361
CciI TCATGA 1 cut(s) 661
Cfr10I RCCGGY 1 cut(s) 434
Cfr13I GGNCC 4 cut(s) 221, 457, 968, 1366
CseI GACGC 1 cut(s) 413
Csp6I GTAC 7 cut(s) 25, 613, 838, 845, 892, 1287, 1358
CviQI GTAC 7 cut(s) 25, 613, 838, 845, 892, 1287, 1358
DdeI CTNAG 1 cut(s) 408
DpnI GATC 6 cut(s) 164, 236, 660, 902, 1092, 1614
DpnII GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
DriI GACNNNNNGTC 2 cut(s) 384, 409
EaeI YGGCCR 2 cut(s) 3, 255
Eam1104I CTCTTC 4 cut(s) 149, 165, 276, 1488
Eam1105I GACNNNNNGTC 2 cut(s) 384, 409
EarI CTCTTC 4 cut(s) 149, 165, 276, 1488
Eco147I AGGCCT 2 cut(s) 152, 1111
Eco47I GGWCC 3 cut(s) 221, 457, 968
Eco57I CTGAAG 2 cut(s) 316, 1068
EcoO109I RGGNCCY 3 cut(s) 221, 457, 1366
EcoRII CCWGG 1 cut(s) 1401
FalI AAGNNNNNCTT 2 cut(s) 1353, 1385
FaqI GGGAC 2 cut(s) 391, 443
FbaI TGATCA 1 cut(s) 1612
FblI GTMKAC 1 cut(s) 413
FspBI CTAG 5 cut(s) 512, 704, 785, 1166, 1452
HaeIII GGCC 5 cut(s) 5, 152, 257, 1111, 1368
HapII CCGG 3 cut(s) 6, 375, 435
HgaI GACGC 1 cut(s) 413
HincII GTYRAC 3 cut(s) 294, 300, 414
HindII GTYRAC 3 cut(s) 294, 300, 414
HinfI GANTC 8 cut(s) 284, 410, 422, 593, 700, 1132, 1189, 1559
HpaII CCGG 3 cut(s) 6, 375, 435
HphI GGTGA 1 cut(s) 637
Hpy166II GTNNAC 5 cut(s) 27, 294, 300, 414, 645
Hpy188I TCNGA 6 cut(s) 247, 421, 674, 695, 1087, 1256
Hpy188III TCNNGA 9 cut(s) 121, 316, 446, 620, 662, 796, 868, 955, 1180
Hpy8I GTNNAC 5 cut(s) 27, 294, 300, 414, 645
Hpy99I CGWCG 2 cut(s) 390, 1352
HpyAV CCTTC 5 cut(s) 401, 448, 1055, 1092, 1330
HpyCH4III ACNGT 2 cut(s) 211, 1286
HpyCH4IV ACGT 7 cut(s) 17, 296, 571, 847, 1233, 1289, 1356
HpyF10VI GCNNNNNNNGC 5 cut(s) 380, 404, 490, 857, 1388
HpyF3I CTNAG 1 cut(s) 408
HpySE526I ACGT 7 cut(s) 17, 296, 571, 847, 1233, 1289, 1356
KpnI GGTACC 1 cut(s) 895
Ksp22I TGATCA 1 cut(s) 1612
Kzo9I GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
LguI GCTCTTC 1 cut(s) 165
LmnI GCTCC 3 cut(s) 62, 1006, 1256
Lsp1109I GCAGC 7 cut(s) 255, 336, 345, 367, 1290, 1446, 1482
LweI GCATC 1 cut(s) 168
MaeI CTAG 5 cut(s) 512, 704, 785, 1166, 1452
MaeII ACGT 7 cut(s) 17, 296, 571, 847, 1233, 1289, 1356
MaeIII GTNAC 6 cut(s) 213, 526, 754, 1229, 1290, 1309
MalI GATC 6 cut(s) 164, 236, 660, 902, 1092, 1614
MboI GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
MfeI CAATTG 1 cut(s) 1305
MflI RGATCY 2 cut(s) 234, 900
MhlI GDGCHC 2 cut(s) 59, 853
MlsI TGGCCA 1 cut(s) 257
MluNI TGGCCA 1 cut(s) 257
MlyI GAGTC 3 cut(s) 293, 419, 1568
MmeI TCCRAC 3 cut(s) 859, 984, 1528
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 5 cut(s) 564, 825, 1203, 1332, 1412
MslI CAYNNNNRTG 2 cut(s) 506, 1316
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 358
MspI CCGG 3 cut(s) 6, 375, 435
MspR9I CCNGG 2 cut(s) 376, 1403
MunI CAATTG 1 cut(s) 1305
Mva1269I GAATGC 1 cut(s) 962
MvaI CCWGG 1 cut(s) 1403
MwoI GCNNNNNNNGC 5 cut(s) 380, 404, 490, 857, 1388
NciI CCSGG 1 cut(s) 376
NdeII GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
NheI GCTAGC 1 cut(s) 511
NlaIV GGNNCC 5 cut(s) 430, 459, 893, 997, 1103
NmeAIII GCCGAG 2 cut(s) 331, 1066
NmuCI GTSAC 3 cut(s) 526, 754, 1309
NspI RCATGY 4 cut(s) 527, 947, 1013, 1574
PaeI GCATGC 1 cut(s) 947
PagI TCATGA 1 cut(s) 661
PceI AGGCCT 2 cut(s) 152, 1111
PciSI GCTCTTC 1 cut(s) 165
PcsI WCGNNNNNNNCGW 2 cut(s) 293, 1353
PctI GAATGC 1 cut(s) 962
PfeI GAWTC 5 cut(s) 422, 593, 700, 1132, 1189
PflMI CCANNNNNTGG 1 cut(s) 264
PleI GAGTC 3 cut(s) 292, 418, 1567
PpsI GAGTC 3 cut(s) 292, 418, 1567
Ppu21I YACGTR 2 cut(s) 848, 1357
PpuMI RGGWCCY 2 cut(s) 221, 457
PsiI TTATAA 2 cut(s) 1016, 1250
Psp1406I AACGTT 1 cut(s) 17
Psp5II RGGWCCY 2 cut(s) 221, 457
Psp6I CCWGG 1 cut(s) 1401
PspGI CCWGG 1 cut(s) 1401
PspN4I GGNNCC 5 cut(s) 430, 459, 893, 997, 1103
PspPI GGNCC 4 cut(s) 221, 457, 968, 1366
PspPPI RGGWCCY 2 cut(s) 221, 457
PsrI GAACNNNNNNTAC 2 cut(s) 1432, 1464
PstI CTGCAG 1 cut(s) 1280
PstNI CAGNNNCTG 1 cut(s) 361
PsuI RGATCY 2 cut(s) 234, 900
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 7 cut(s) 26, 614, 839, 846, 893, 1288, 1359
RsaNI GTAC 7 cut(s) 25, 613, 838, 845, 892, 1287, 1358
RseI CAYNNNNRTG 2 cut(s) 506, 1316
SalI GTCGAC 1 cut(s) 412
SapI GCTCTTC 1 cut(s) 165
SaqAI TTAA 5 cut(s) 564, 825, 1203, 1332, 1412
Sau3AI GATC 6 cut(s) 162, 234, 658, 900, 1090, 1612
Sau96I GGNCC 4 cut(s) 221, 457, 968, 1366
SchI GAGTC 3 cut(s) 293, 419, 1568
ScrFI CCNGG 2 cut(s) 376, 1403
SduI GDGCHC 2 cut(s) 59, 853
SfaNI GCATC 1 cut(s) 168
SfcI CTRYAG 4 cut(s) 225, 597, 1276, 1282
SinI GGWCC 3 cut(s) 221, 457, 968
SmiMI CAYNNNNRTG 2 cut(s) 506, 1316
SmlI CTYRAG 2 cut(s) 119, 1542
SmoI CTYRAG 2 cut(s) 119, 1542
SphI GCATGC 1 cut(s) 947
SseBI AGGCCT 2 cut(s) 152, 1111
SsiI CCGC 5 cut(s) 648, 860, 1406, 1421, 1601
SspI AATATT 2 cut(s) 1196, 1344
SspMI CTAG 5 cut(s) 512, 704, 785, 1166, 1452
StuI AGGCCT 2 cut(s) 152, 1111
StyD4I CCNGG 2 cut(s) 374, 1401
TaaI ACNGT 2 cut(s) 211, 1286
TaiI ACGT 7 cut(s) 20, 299, 574, 850, 1236, 1292, 1359
TaqI TCGA 6 cut(s) 87, 147, 287, 413, 1114, 1347
TaqII GACCGA 1 cut(s) 956
TatI WGTACW 3 cut(s) 24, 612, 837
TauI GCSGC 3 cut(s) 1409, 1424, 1603
TfiI GAWTC 5 cut(s) 422, 593, 700, 1132, 1189
Tru1I TTAA 5 cut(s) 564, 825, 1203, 1332, 1412
Tru9I TTAA 5 cut(s) 564, 825, 1203, 1332, 1412
TscAI CASTG 1 cut(s) 112
TseFI GTSAC 3 cut(s) 526, 754, 1309
TseI GCWGC 7 cut(s) 268, 324, 355, 358, 1278, 1434, 1470
Tsp45I GTSAC 3 cut(s) 526, 754, 1309
TspDTI ATGAA 4 cut(s) 61, 490, 781, 1124
TspGWI ACGGA 2 cut(s) 49, 1555
TspRI CASTG 1 cut(s) 112
Van91I CCANNNNNTGG 1 cut(s) 264
VpaK11BI GGWCC 3 cut(s) 221, 457, 968
XapI RAATTY 3 cut(s) 820, 1153, 1581
XceI RCATGY 4 cut(s) 527, 947, 1013, 1574
XmiI GTMKAC 1 cut(s) 413
XspI CTAG 5 cut(s) 512, 704, 785, 1166, 1452
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.