FvH4_1g01510

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016720
Physical Location & Seq
Forward (+)
799524 .. 800378
855 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g01510.t1

Sequence Viewer

Length: 855 bp
ATGACCACACCAAACACCGCCGCGCCCACCGTCGCATTCACCACTCCACCAATCTACGCCGCCCGTCTCCGCCACCTCCTCACCCTCACCGGCTTCACTCCCCTCTCCTCTCCCACACTCACCGTCCACCACACCGCATCCACCATCTCCGCTCTCAAGCCCCACCTCTCGCCTCCCTCCCTCGACCTCTTCTCCGCCGTCGCCTTCCCTTCCCGCACTGCCATCGCCGCCTTCTCCGCCGCCGCAGACGGCCTCTCTCCTCCTCTCCTCTCCCCACACGGCGCCGTCTTCACCGTCGCCGCGCTCGGCAGGGACGCCGAGCTCATGGACGACGCCTTCGTCTCCAAGCTCAGTCCACACCGAGTGAGGATTCTGGTACCGGAGACCGCCACGCCGAGCGCGATGGTTCACTCGCTCGGCGACGGCGGCGGACGGAGGATTTTATGTCCGGTTCCGGCGGTGTTGGGGCTGACTGAGCCTCCGGTGGTGCCGGATTTTCTCCGGGAACTCGGAGAAAGCGGTTGGGCTCCGGTTCGGGTCAATGCGTACGAAACTCGTTGGGCCGGGTCGGAGTGTGCGGCCCAAGTGGTGGAGAGAATTAAGGCGGGTAATTTGGATGCGATTGTGTTTACTAGCACTGCTGAGGTGGAGGGGTTACTGAAGAGCTTCAAAAAAATTGGGCTGCGCTGGGAGAGTGTCAAGAGATTATGCCCAAAGCTGCTCGTGGCGGCGCACGGGCCTGTTACGGCCGCCGGAGCACGAATGCTTGGAGTGGAAGTAGATGTGGTGAGTTCTCAGTTTGGTAGTTTCCAAGGTGTTGTTGATTCTTTGCATGATGAGATTTTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

285

Amino Acids

29.88

Weight (kDa)

7.13

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 23 - 267 5.5e-19 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
Acc65I GGTACC 1 cut(s) 376
AccB1I GGYRCC 3 cut(s) 281, 376, 487
AccB7I CCANNNNNTGG 1 cut(s) 589
AccBSI CCGCTC 1 cut(s) 152
AccII CGCG 3 cut(s) 23, 302, 401
AcoI YGGCCR 1 cut(s) 747
AcuI CTGAAG 1 cut(s) 680
AcyI GRCGYC 3 cut(s) 282, 315, 333
AdeI CACNNNGTG 1 cut(s) 364
AfaI GTAC 2 cut(s) 378, 548
AfiI CCNNNNNNNGG 1 cut(s) 589
AgsI TTSAA 2 cut(s) 670, 847
AluBI AGCT 5 cut(s) 322, 349, 666, 718, 851
AluI AGCT 5 cut(s) 322, 349, 666, 718, 851
Alw21I GWGCWC 2 cut(s) 324, 760
Alw26I GTCTC 3 cut(s) 71, 346, 377
AoxI GGCC 5 cut(s) 250, 561, 579, 737, 747
ApeKI GCWGC 2 cut(s) 682, 718
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 665
Asp718I GGTACC 1 cut(s) 376
AspLEI GCGC 6 cut(s) 25, 284, 304, 401, 687, 733
AspS9I GGNCC 3 cut(s) 561, 580, 737
AsuC2I CCSGG 2 cut(s) 503, 565
AsuHPI GGTGA 6 cut(s) 31, 73, 79, 112, 283, 799
BanI GGYRCC 3 cut(s) 281, 376, 487
BanII GRGCYC 2 cut(s) 324, 529
BauI CACGAG 1 cut(s) 722
BbsI GAAGAC 1 cut(s) 280
Bbv12I GWGCWC 2 cut(s) 324, 760
BbvCI CCTCAGC 1 cut(s) 642
BbvI GCAGC 2 cut(s) 669, 705
BccI CCATC 3 cut(s) 152, 230, 397
BceAI ACGGC 6 cut(s) 182, 265, 269, 295, 439, 762
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BcnI CCSGG 2 cut(s) 503, 565
BcoDI GTCTC 3 cut(s) 71, 346, 377
BfaI CTAG 1 cut(s) 633
BfoI RGCGCY 1 cut(s) 285
Bme1390I CCNGG 2 cut(s) 503, 565
BmgT120I GGNCC 3 cut(s) 561, 580, 737
BmiI GGNNCC 5 cut(s) 283, 378, 453, 489, 528
BmrFI CCNGG 2 cut(s) 503, 565
BmsI GCATC 2 cut(s) 146, 607
BpiI GAAGAC 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 642
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 2 cut(s) 503, 565
BsaHI GRCGYC 3 cut(s) 282, 315, 333
BsaI GGTCTC 1 cut(s) 377
BsaJI CCNNGG 1 cut(s) 811
BsaWI WCCGGW 4 cut(s) 379, 448, 481, 529
BsaXI ACNNNNNCTCC 6 cut(s) 176, 206, 463, 493, 762, 792
Bsc4I CCNNNNNNNGG 1 cut(s) 589
Bse118I RCCGGY 1 cut(s) 89
BseDI CCNNGG 1 cut(s) 811
BseGI GGATG 2 cut(s) 137, 622
BseLI CCNNNNNNNGG 1 cut(s) 589
BseMII CTCAG 4 cut(s) 364, 465, 633, 809
BseRI GAGGAG 5 cut(s) 68, 97, 249, 252, 257
BseX3I CGGCCG 1 cut(s) 747
BseXI GCAGC 2 cut(s) 669, 705
BseYI CCCAGC 1 cut(s) 687
Bsh1236I CGCG 3 cut(s) 23, 302, 401
Bsh1285I CGRYCG 1 cut(s) 750
BshFI GGCC 5 cut(s) 252, 563, 581, 739, 749
BshNI GGYRCC 3 cut(s) 281, 376, 487
BsiEI CGRYCG 1 cut(s) 750
BsiHKAI GWGCWC 2 cut(s) 324, 760
BsiWI CGTACG 1 cut(s) 546
BslFI GGGAC 1 cut(s) 326
BslI CCNNNNNNNGG 1 cut(s) 589
BsmAI GTCTC 3 cut(s) 71, 346, 377
BsmBI CGTCTC 2 cut(s) 71, 346
BsmFI GGGAC 1 cut(s) 326
BsmI GAATGC 2 cut(s) 35, 768
BsnI GGCC 5 cut(s) 252, 563, 581, 739, 749
Bso31I GGTCTC 1 cut(s) 377
Bsp1286I GDGCHC 3 cut(s) 324, 529, 760
BspANI GGCC 5 cut(s) 252, 563, 581, 739, 749
BspCNI CTCAG 4 cut(s) 363, 466, 634, 808
BspFNI CGCG 3 cut(s) 23, 302, 401
BspLI GGNNCC 5 cut(s) 283, 378, 453, 489, 528
BspQI GCTCTTC 1 cut(s) 656
BspT107I GGYRCC 3 cut(s) 281, 376, 487
BspTNI GGTCTC 1 cut(s) 377
BsrBI CCGCTC 1 cut(s) 152
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssECI CCNNGG 1 cut(s) 811
BssNI GRCGYC 3 cut(s) 282, 315, 333
BssSI CACGAG 1 cut(s) 722
BssT1I CCWWGG 1 cut(s) 811
Bst2BI CACGAG 1 cut(s) 722
Bst4CI ACNGT 3 cut(s) 31, 124, 295
Bst6I CTCTTC 2 cut(s) 194, 656
BstACI GRCGYC 3 cut(s) 282, 315, 333
BstDEI CTNAG 5 cut(s) 350, 474, 642, 795, 852
BstF5I GGATG 2 cut(s) 137, 622
BstFNI CGCG 3 cut(s) 23, 302, 401
BstH2I RGCGCY 1 cut(s) 285
BstHHI GCGC 6 cut(s) 25, 284, 304, 401, 687, 733
BstMAI GTCTC 3 cut(s) 71, 346, 377
BstMCI CGRYCG 1 cut(s) 750
BstMWI GCNNNNNNNGC 5 cut(s) 227, 236, 426, 475, 755
BstSCI CCNGG 2 cut(s) 501, 563
BstUI CGCG 3 cut(s) 23, 302, 401
BstV1I GCAGC 2 cut(s) 669, 705
BstV2I GAAGAC 1 cut(s) 280
BstZI CGGCCG 1 cut(s) 747
BsuRI GGCC 5 cut(s) 252, 563, 581, 739, 749
BtgZI GCGATG 2 cut(s) 208, 416
BtsCI GGATG 2 cut(s) 137, 622
BtsI GCAGTG 2 cut(s) 216, 636
BtsIMutI CAGTG 2 cut(s) 216, 636
CfoI GCGC 6 cut(s) 25, 284, 304, 401, 687, 733
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 3 cut(s) 561, 580, 737
CseI GACGC 2 cut(s) 323, 341
Csp6I GTAC 2 cut(s) 377, 547
CviAII CATG 2 cut(s) 325, 833
CviQI GTAC 2 cut(s) 377, 547
DdeI CTNAG 5 cut(s) 350, 474, 642, 795, 852
DinI GGCGCC 1 cut(s) 283
DraIII CACNNNGTG 1 cut(s) 364
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 747
EagI CGGCCG 1 cut(s) 747
Eam1104I CTCTTC 2 cut(s) 194, 656
EarI CTCTTC 2 cut(s) 194, 656
EciI GGCGGA 4 cut(s) 59, 184, 226, 444
Ecl136II GAGCTC 1 cut(s) 322
EclXI CGGCCG 1 cut(s) 747
Eco130I CCWWGG 1 cut(s) 811
Eco24I GRGCYC 2 cut(s) 324, 529
Eco31I GGTCTC 1 cut(s) 377
Eco52I CGGCCG 1 cut(s) 747
Eco53kI GAGCTC 1 cut(s) 322
Eco57I CTGAAG 1 cut(s) 680
EcoICRI GAGCTC 1 cut(s) 322
EcoT14I CCWWGG 1 cut(s) 811
EcoT38I GRGCYC 2 cut(s) 324, 529
EgeI GGCGCC 1 cut(s) 283
EheI GGCGCC 1 cut(s) 283
ErhI CCWWGG 1 cut(s) 811
Esp3I CGTCTC 2 cut(s) 71, 346
FaeI CATG 2 cut(s) 328, 836
FaiI YATR 4 cut(s) 326, 445, 709, 834
FaqI GGGAC 1 cut(s) 326
FatI CATG 2 cut(s) 324, 832
FauI CCCGC 2 cut(s) 221, 598
FokI GGATG 2 cut(s) 124, 629
FriOI GRGCYC 2 cut(s) 324, 529
FspBI CTAG 1 cut(s) 633
GlaI GCGC 6 cut(s) 24, 283, 303, 400, 686, 732
GsaI CCCAGC 1 cut(s) 691
HaeII RGCGCY 1 cut(s) 285
HaeIII GGCC 5 cut(s) 252, 563, 581, 739, 749
HgaI GACGC 2 cut(s) 323, 341
HhaI GCGC 6 cut(s) 25, 284, 304, 401, 687, 733
Hin1I GRCGYC 3 cut(s) 282, 315, 333
Hin1II CATG 2 cut(s) 328, 836
Hin6I GCGC 6 cut(s) 23, 282, 302, 399, 685, 731
HinP1I GCGC 6 cut(s) 23, 282, 302, 399, 685, 731
HindIII AAGCTT 1 cut(s) 849
HinfI GANTC 2 cut(s) 370, 824
HphI GGTGA 6 cut(s) 31, 73, 79, 112, 283, 799
Hpy166II GTNNAC 4 cut(s) 127, 356, 409, 630
Hpy188I TCNGA 2 cut(s) 512, 571
Hpy188III TCNNGA 1 cut(s) 700
Hpy8I GTNNAC 4 cut(s) 127, 356, 409, 630
Hpy99I CGWCG 5 cut(s) 35, 203, 299, 335, 425
HpyAV CCTTC 4 cut(s) 214, 219, 241, 346
HpyCH4III ACNGT 3 cut(s) 31, 124, 295
HpyCH4V TGCA 1 cut(s) 832
HpyF10VI GCNNNNNNNGC 5 cut(s) 227, 236, 426, 475, 755
HpyF3I CTNAG 5 cut(s) 350, 474, 642, 795, 852
Hsp92I GRCGYC 3 cut(s) 282, 315, 333
Hsp92II CATG 2 cut(s) 328, 836
HspAI GCGC 6 cut(s) 23, 282, 302, 399, 685, 731
KasI GGCGCC 1 cut(s) 281
KpnI GGTACC 1 cut(s) 380
LguI GCTCTTC 1 cut(s) 656
LmnI GCTCC 2 cut(s) 532, 755
Lsp1109I GCAGC 2 cut(s) 669, 705
LweI GCATC 2 cut(s) 146, 607
MaeI CTAG 1 cut(s) 633
MaeIII GTNAC 2 cut(s) 654, 742
MbiI CCGCTC 1 cut(s) 152
MboII GAAGA 3 cut(s) 181, 280, 673
MhlI GDGCHC 3 cut(s) 324, 529, 760
MluCI AATT 3 cut(s) 597, 610, 675
Mly113I GGCGCC 1 cut(s) 282
MmeI TCCRAC 1 cut(s) 549
MroXI GAANNNNTTC 1 cut(s) 665
MseI TTAA 1 cut(s) 600
MspR9I CCNGG 2 cut(s) 503, 565
Mva1269I GAATGC 2 cut(s) 35, 768
MvnI CGCG 3 cut(s) 23, 302, 401
MwoI GCNNNNNNNGC 5 cut(s) 227, 236, 426, 475, 755
NarI GGCGCC 1 cut(s) 282
NciI CCSGG 2 cut(s) 503, 565
NlaIII CATG 2 cut(s) 328, 836
NlaIV GGNNCC 5 cut(s) 283, 378, 453, 489, 528
NmeAIII GCCGAG 4 cut(s) 285, 343, 396, 420
PciSI GCTCTTC 1 cut(s) 656
PcsI WCGNNNNNNNCGW 2 cut(s) 336, 398
PctI GAATGC 2 cut(s) 35, 768
PdmI GAANNNNTTC 1 cut(s) 665
PfeI GAWTC 2 cut(s) 370, 824
Pfl23II CGTACG 1 cut(s) 546
PflMI CCANNNNNTGG 1 cut(s) 589
PfoI TCCNGGA 1 cut(s) 501
PluTI GGCGCC 1 cut(s) 285
Psp124BI GAGCTC 1 cut(s) 324
PspFI CCCAGC 1 cut(s) 687
PspLI CGTACG 1 cut(s) 546
PspN4I GGNNCC 5 cut(s) 283, 378, 453, 489, 528
PspPI GGNCC 3 cut(s) 561, 580, 737
RsaI GTAC 2 cut(s) 378, 548
RsaNI GTAC 2 cut(s) 377, 547
SacI GAGCTC 1 cut(s) 324
SapI GCTCTTC 1 cut(s) 656
SaqAI TTAA 1 cut(s) 600
Sau96I GGNCC 3 cut(s) 561, 580, 737
ScrFI CCNGG 2 cut(s) 503, 565
SduI GDGCHC 3 cut(s) 324, 529, 760
SfaNI GCATC 2 cut(s) 146, 607
SfoI GGCGCC 1 cut(s) 283
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
Sse9I AATT 3 cut(s) 597, 610, 675
SspDI GGCGCC 1 cut(s) 281
SspMI CTAG 1 cut(s) 633
SstI GAGCTC 1 cut(s) 324
StyD4I CCNGG 2 cut(s) 501, 563
StyI CCWWGG 1 cut(s) 811
TaaI ACNGT 3 cut(s) 31, 124, 295
TaqI TCGA 1 cut(s) 183
TasI AATT 3 cut(s) 597, 610, 675
TfiI GAWTC 2 cut(s) 370, 824
Tru1I TTAA 1 cut(s) 600
Tru9I TTAA 1 cut(s) 600
TscAI CASTG 2 cut(s) 223, 643
TseI GCWGC 2 cut(s) 682, 718
TspGWI ACGGA 1 cut(s) 448
TspRI CASTG 2 cut(s) 223, 643
Van91I CCANNNNNTGG 1 cut(s) 589
XmnI GAANNNNTTC 1 cut(s) 665
XspI CTAG 1 cut(s) 633
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.