MD15G1157700.v1.1

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0016720
Physical Location & Seq
Forward (+)
11722801 .. 11723670
870 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1157700.v1.1.491

Sequence Viewer

Length: 870 bp
ATGGCCGCCGCGCATGGCAGTGGCAGCATCCCCACCGTCGCATTCACCACCCCTCCAAACTACGCCGCCAGGCTGGCCCACCTCCTCACCCTCAAAGGCTTCAAACCCCTCTCCGCCCCCACACTCATCGTTCAGCCCACACCGTCCACCGTAGCCGCCCTCAAACCCCACCTCTCACCCCCTTCCCTCGATCTCTTCTCCGCCGTCGCCTTCCCTTCACGCACAGCCGTCGCCGCGTTCTCATCCGCCGCCGCAGACGTCCCCCACCCTCTCCTCTCCCCGCACGGGGACGTATTCACGGTCGCCGCGCTGGGAAAGGACTCCGAGCTCATAGACGACAGTTTCGTCCACAGGCTCTGTCCCAATCCTGACCGAATCAGAATTTTAGTGCCTTTGACCGCGACCCCGAACGCATTGGTCCAATCGCTCGAAGACGGGCGCGGTCGAAGGGTGTTGTGTCCAGTCCCGCTAGTAGTGGGACTGGTCGAGCCTCCCGTGGTCCCACATTTTCTTCGGGACTTGGAGGCCAAGCATTGGGTTCCAGTTAGGGTAAACGCGTACGAGACGCGATGGGCCGGCCCGGGCTGTGCCGAGGAAGTGGTGGAGAAGATCGAAGATGAAGCGTTGGATGCGATTGTGTTTACGAGCACGGCGGAGGTGGAGGGGTTGCTAAAGAGCTTCAAAGAGTTTGGGCTGGACTGGGAGACCGCCAAAAGGAGGTGCCCCAAAATGCTGGTGGCGGCCCATGGGCCCATCACGGCAGCCGGGGCTCAGATGCTTGGAGTTGGAGTTGATTTGGTGAGTTCTCAGTTTGGTAGCTTTCAAGGAGTTGTTGATGCTTTGCACGCTGAGATTTTGAGATTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

290

Amino Acids

30.68

Weight (kDa)

6.05

Isoelectric Point (pI)

38.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 24 - 271 2.2e-18 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 720
AccB7I CCANNNNNTGG 1 cut(s) 534
AccII CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 381
AcyI GRCGYC 1 cut(s) 258
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 5 cut(s) 285, 286, 534, 714, 717
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 3 cut(s) 103, 682, 824
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 3 cut(s) 328, 678, 819
AluI AGCT 3 cut(s) 328, 678, 819
Alw21I GWGCWC 2 cut(s) 330, 650
Alw26I GTCTC 2 cut(s) 557, 698
AlwNI CAGNNNCTG 1 cut(s) 357
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 7 cut(s) 3, 75, 525, 573, 577, 741, 749
ApaI GGGCCC 1 cut(s) 753
ApeKI GCWGC 2 cut(s) 24, 761
ApoI RAATTY 1 cut(s) 381
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
AspLEI GCGC 3 cut(s) 13, 310, 441
AspS9I GGNCC 8 cut(s) 76, 418, 499, 573, 578, 742, 749, 750
AsuC2I CCSGG 3 cut(s) 581, 582, 766
AsuHPI GGTGA 4 cut(s) 37, 79, 168, 811
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 2 cut(s) 418, 499
BaeGI GKGCMC 2 cut(s) 725, 753
BanI GGYRCC 1 cut(s) 720
BanII GRGCYC 3 cut(s) 330, 753, 772
BbsI GAAGAC 1 cut(s) 438
Bbv12I GWGCWC 2 cut(s) 330, 650
BbvI GCAGC 2 cut(s) 36, 773
BccI CCATC 2 cut(s) 564, 761
BceAI ACGGC 4 cut(s) 188, 212, 666, 774
BcgI CGANNNNNNTGC 2 cut(s) 211, 245
BciT130I CCWGG 1 cut(s) 70
BcnI CCSGG 3 cut(s) 581, 582, 766
BcoDI GTCTC 2 cut(s) 557, 698
BfaI CTAG 1 cut(s) 470
BglI GCCNNNNNGGC 1 cut(s) 74
Bme1390I CCNGG 4 cut(s) 70, 581, 582, 766
Bme18I GGWCC 2 cut(s) 418, 499
BmeT110I CYCGRG 1 cut(s) 580
BmgT120I GGNCC 8 cut(s) 76, 418, 499, 573, 578, 742, 749, 750
BmiI GGNNCC 4 cut(s) 501, 540, 722, 751
BmrFI CCNGG 4 cut(s) 70, 581, 582, 766
BmrI ACTGGG 1 cut(s) 709
BmsI GCATC 4 cut(s) 36, 619, 765, 826
BmuI ACTGGG 1 cut(s) 709
BpiI GAAGAC 1 cut(s) 438
BpuMI CCSGG 3 cut(s) 581, 582, 766
BsaHI GRCGYC 1 cut(s) 258
BsaI GGTCTC 1 cut(s) 698
BsaJI CCNNGG 5 cut(s) 495, 580, 591, 745, 765
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 774, 804
Bsc4I CCNNNNNNNGG 5 cut(s) 285, 286, 534, 714, 717
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 4 cut(s) 461, 486, 542, 704
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 5 cut(s) 495, 580, 591, 745, 765
BseGI GGATG 3 cut(s) 27, 242, 634
BseLI CCNNNNNNNGG 5 cut(s) 285, 286, 534, 714, 717
BseMII CTCAG 3 cut(s) 785, 821, 840
BseNI ACTGG 4 cut(s) 461, 486, 542, 704
BseRI GAGGAG 2 cut(s) 74, 263
BseSI GKGCMC 2 cut(s) 725, 753
BseXI GCAGC 2 cut(s) 36, 773
BseYI CCCAGC 1 cut(s) 310
Bsh1236I CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
Bsh1285I CGRYCG 2 cut(s) 303, 445
BshFI GGCC 7 cut(s) 5, 77, 527, 575, 579, 743, 751
BshNI GGYRCC 1 cut(s) 720
BsiEI CGRYCG 2 cut(s) 303, 445
BsiHKAI GWGCWC 2 cut(s) 330, 650
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 3 cut(s) 576, 581, 765
BsiWI CGTACG 1 cut(s) 558
BslFI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
BslI CCNNNNNNNGG 5 cut(s) 285, 286, 534, 714, 717
BsmAI GTCTC 2 cut(s) 557, 698
BsmBI CGTCTC 1 cut(s) 557
BsmFI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 7 cut(s) 5, 77, 527, 575, 579, 743, 751
Bso31I GGTCTC 1 cut(s) 698
BsoBI CYCGRG 1 cut(s) 580
Bsp120I GGGCCC 1 cut(s) 749
Bsp1286I GDGCHC 5 cut(s) 330, 650, 725, 753, 772
Bsp143I GATC 2 cut(s) 190, 609
Bsp19I CCATGG 1 cut(s) 745
BspANI GGCC 7 cut(s) 5, 77, 527, 575, 579, 743, 751
BspCNI CTCAG 3 cut(s) 784, 820, 841
BspFNI CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
BspLI GGNNCC 4 cut(s) 501, 540, 722, 751
BspT107I GGYRCC 1 cut(s) 720
BspTNI GGTCTC 1 cut(s) 698
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 4 cut(s) 461, 486, 542, 704
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 5 cut(s) 495, 580, 591, 745, 765
BssMI GATC 2 cut(s) 190, 609
BssNI GRCGYC 1 cut(s) 258
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 5 cut(s) 37, 144, 151, 301, 341
Bst6I CTCTTC 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 258
BstC8I GCNNGC 3 cut(s) 75, 577, 846
BstDEI CTNAG 3 cut(s) 771, 807, 849
BstDSI CCRYGG 2 cut(s) 495, 745
BstF5I GGATG 3 cut(s) 27, 242, 634
BstFNI CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
BstHHI GCGC 3 cut(s) 13, 310, 441
BstKTI GATC 2 cut(s) 193, 612
BstMAI GTCTC 2 cut(s) 557, 698
BstMBI GATC 2 cut(s) 190, 609
BstMCI CGRYCG 2 cut(s) 303, 445
BstMWI GCNNNNNNNGC 6 cut(s) 24, 74, 233, 629, 767, 845
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 4 cut(s) 68, 579, 580, 764
BstSLI GKGCMC 2 cut(s) 725, 753
BstUI CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
BstV1I GCAGC 2 cut(s) 36, 773
BstV2I GAAGAC 1 cut(s) 438
BstXI CCANNNNNNTGG 1 cut(s) 733
BsuRI GGCC 7 cut(s) 5, 77, 527, 575, 579, 743, 751
BtgI CCRYGG 2 cut(s) 495, 745
BtgZI GCGATG 1 cut(s) 583
BtsCI GGATG 3 cut(s) 27, 242, 634
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 1 cut(s) 25
Cac8I GCNNGC 3 cut(s) 75, 577, 846
CaiI CAGNNNCTG 1 cut(s) 357
CfoI GCGC 3 cut(s) 13, 310, 441
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 8 cut(s) 76, 418, 499, 573, 578, 742, 749, 750
Cfr9I CCCGGG 1 cut(s) 580
CseI GACGC 1 cut(s) 574
Csp6I GTAC 1 cut(s) 559
CviAII CATG 2 cut(s) 14, 746
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 3 cut(s) 771, 807, 849
DpnI GATC 2 cut(s) 192, 611
DpnII GATC 2 cut(s) 190, 609
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 200
EarI CTCTTC 1 cut(s) 200
EciI GGCGGA 4 cut(s) 103, 190, 235, 668
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 3 cut(s) 330, 753, 772
Eco31I GGTCTC 1 cut(s) 698
Eco47I GGWCC 2 cut(s) 418, 499
Eco53kI GAGCTC 1 cut(s) 328
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 328
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 745
EcoT38I GRGCYC 3 cut(s) 330, 753, 772
ErhI CCWWGG 1 cut(s) 745
Esp3I CGTCTC 1 cut(s) 557
FaeI CATG 2 cut(s) 17, 749
FaiI YATR 3 cut(s) 15, 332, 747
FaqI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
FatI CATG 2 cut(s) 13, 745
FauI CCCGC 2 cut(s) 288, 474
FokI GGATG 3 cut(s) 14, 229, 641
FriOI GRGCYC 3 cut(s) 330, 753, 772
FseI GGCCGGCC 1 cut(s) 579
FspBI CTAG 1 cut(s) 470
GlaI GCGC 3 cut(s) 12, 309, 440
GsaI CCCAGC 1 cut(s) 314
HaeIII GGCC 7 cut(s) 5, 77, 527, 575, 579, 743, 751
HapII CCGG 3 cut(s) 576, 581, 765
HgaI GACGC 1 cut(s) 574
HhaI GCGC 3 cut(s) 13, 310, 441
Hin1I GRCGYC 1 cut(s) 258
Hin1II CATG 2 cut(s) 17, 749
Hin6I GCGC 3 cut(s) 11, 308, 439
HinP1I GCGC 3 cut(s) 11, 308, 439
HinfI GANTC 2 cut(s) 320, 375
HpaII CCGG 3 cut(s) 576, 581, 765
HphI GGTGA 4 cut(s) 37, 79, 168, 811
Hpy166II GTNNAC 4 cut(s) 147, 349, 553, 642
Hpy188I TCNGA 3 cut(s) 325, 380, 774
Hpy188III TCNNGA 2 cut(s) 368, 515
Hpy8I GTNNAC 4 cut(s) 147, 349, 553, 642
Hpy99I CGWCG 3 cut(s) 41, 209, 233
HpyAV CCTTC 4 cut(s) 192, 220, 225, 441
HpyCH4III ACNGT 5 cut(s) 37, 144, 151, 301, 341
HpyCH4IV ACGT 2 cut(s) 258, 291
HpyCH4V TGCA 1 cut(s) 844
HpyF10VI GCNNNNNNNGC 6 cut(s) 24, 74, 233, 629, 767, 845
HpyF3I CTNAG 3 cut(s) 771, 807, 849
HpySE526I ACGT 2 cut(s) 258, 291
Hsp92I GRCGYC 1 cut(s) 258
Hsp92II CATG 2 cut(s) 17, 749
HspAI GCGC 3 cut(s) 11, 308, 439
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Kzo9I GATC 2 cut(s) 190, 609
Lsp1109I GCAGC 2 cut(s) 36, 773
LweI GCATC 4 cut(s) 36, 619, 765, 826
MaeI CTAG 1 cut(s) 470
MaeII ACGT 2 cut(s) 258, 291
MalI GATC 2 cut(s) 192, 611
MboI GATC 2 cut(s) 190, 609
MboII GAAGA 5 cut(s) 187, 443, 503, 619, 626
MhlI GDGCHC 5 cut(s) 330, 650, 725, 753, 772
MluCI AATT 1 cut(s) 381
MluI ACGCGT 1 cut(s) 555
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 2 cut(s) 606, 766
MroNI GCCGGC 1 cut(s) 575
MseI TTAA 1 cut(s) 868
MslI CAYNNNNRTG 1 cut(s) 18
MspI CCGG 3 cut(s) 576, 581, 765
MspR9I CCNGG 4 cut(s) 70, 581, 582, 766
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 1 cut(s) 70
MvnI CGCG 7 cut(s) 11, 236, 308, 401, 441, 557, 568
MwoI GCNNNNNNNGC 6 cut(s) 24, 74, 233, 629, 767, 845
NaeI GCCGGC 1 cut(s) 577
NciI CCSGG 3 cut(s) 581, 582, 766
NcoI CCATGG 1 cut(s) 745
NdeII GATC 2 cut(s) 190, 609
NgoMIV GCCGGC 1 cut(s) 575
NlaIII CATG 2 cut(s) 17, 749
NlaIV GGNNCC 4 cut(s) 501, 540, 722, 751
NmeAIII GCCGAG 1 cut(s) 616
PcsI WCGNNNNNNNCGW 2 cut(s) 342, 492
PctI GAATGC 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 577
PfeI GAWTC 1 cut(s) 375
Pfl23II CGTACG 1 cut(s) 558
PflMI CCANNNNNTGG 1 cut(s) 534
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 68
PspLI CGTACG 1 cut(s) 558
PspN4I GGNNCC 4 cut(s) 501, 540, 722, 751
PspOMI GGGCCC 1 cut(s) 749
PspPI GGNCC 8 cut(s) 76, 418, 499, 573, 578, 742, 749, 750
PstNI CAGNNNCTG 1 cut(s) 357
RigI GGCCGGCC 1 cut(s) 579
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 330
SaqAI TTAA 1 cut(s) 868
Sau3AI GATC 2 cut(s) 190, 609
Sau96I GGNCC 8 cut(s) 76, 418, 499, 573, 578, 742, 749, 750
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 4 cut(s) 70, 581, 582, 766
SduI GDGCHC 5 cut(s) 330, 650, 725, 753, 772
SetI ASST 9 cut(s) 84, 174, 261, 294, 330, 660, 680, 722, 821
SfaNI GCATC 4 cut(s) 36, 619, 765, 826
SinI GGWCC 2 cut(s) 418, 499
SmaI CCCGGG 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 18
SrfI GCCCGGGC 1 cut(s) 582
Sse9I AATT 1 cut(s) 381
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 68, 579, 580, 764
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 5 cut(s) 37, 144, 151, 301, 341
TaiI ACGT 2 cut(s) 261, 294
TaqI TCGA 5 cut(s) 189, 429, 445, 486, 612
TaqII GACCGA 1 cut(s) 387
TasI AATT 1 cut(s) 381
TauI GCSGC 9 cut(s) 8, 11, 68, 158, 236, 251, 254, 308, 743
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TscAI CASTG 1 cut(s) 25
TseI GCWGC 2 cut(s) 24, 761
TspDTI ATGAA 1 cut(s) 633
TspMI CCCGGG 1 cut(s) 580
TspRI CASTG 1 cut(s) 25
Van91I CCANNNNNTGG 1 cut(s) 534
VpaK11BI GGWCC 2 cut(s) 418, 499
XapI RAATTY 1 cut(s) 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 580
XspI CTAG 1 cut(s) 470
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.