Rw2G001510

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016720
Physical Location & Seq
Forward (+)
1259928 .. 1260803
876 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G001510.1

Sequence Viewer

Length: 876 bp
ATGGCCACACCAAACACCGCCGTCACCACCACCACCACCCCCACCGTCGCATTCACCACTCCGCCAATCTACGCCGCCCGCCTCCGCCACTTCCTCACCCTCAACAACTTCGCTCCCCTCTCCTCTCCCACACTCACCGTCCACCACACCCCCTCCACCGTCTCCGCCCTCAAACCCCACCTCTCGCCTCCTTCCCTAAACCTCTTCTCCGCCGTCGCCTTCCCTTCCCGCACCGCCATCGCCGCCTTCTCCGCCGCCGCAGACGACGTCGCTCATCCTCTCCTCTCCCCAAACGGCGCCGTCTTCACCGTCGCCGCGCTCGGCAAGGACGCCGAGCTCATCGACGACGGCTTCGTCCGCAAGCTCAGTCGAGATCGTCGCCGTGTCAGAATTCTGGTACCTCCGACCGCCACGCCGAGTGCGTTGGTTCACGCGCTCGGCGACGGCGGAGGCAGGAGGGTGCTGTGTCCGGTACCGGTGGTGTTTGGACTGACCGAGCCTCCGGTGGTGCCGGATTTTCTCCGGGAGCTCGGAGAAAGCGGTTGGGCTCCGGTTCGGGTCAATGCGTACGAGACGAGGTGGGCCGGGCCGGAGTGTGCGGCCCAAGTGGTGGAGAGAATCAAGAACGGGGCTTTGGATGCGATTGTGTTTACTAGCACTGCAGAGGTGGAGGGGTTACTGAAGAGCTTCAAGGAGTTTGGGCTGGGCTGGGAGAGTGTCAAGAAATTGTGCCCGAAACTGCTCGTTGCGGCCCACGGGCCCGTTACTGCCTCCGGAGCCCGAAACCTTGGTGTCGGAGTAGATTTGGTGAGTTCCAAGTTCGACAGTTTTCAAGGTGTTGTTGATTCTCTGCGTTCTGAGATTTTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

291

Amino Acids

30.77

Weight (kDa)

9.07

Isoelectric Point (pI)

42.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 37 - 275 2.1e-17 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 353
AatII GACGTC 1 cut(s) 270
Acc65I GGTACC 2 cut(s) 397, 472
AccB1I GGYRCC 4 cut(s) 296, 397, 472, 508
AccB7I CCANNNNNTGG 1 cut(s) 610
AccII CGCG 2 cut(s) 317, 434
AccIII TCCGGA 1 cut(s) 773
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 390
AcuI CTGAAG 1 cut(s) 701
AcyI GRCGYC 3 cut(s) 267, 297, 330
AfaI GTAC 3 cut(s) 399, 474, 569
AfiI CCNNNNNNNGG 1 cut(s) 610
AgeI ACCGGT 1 cut(s) 475
AgsI TTSAA 3 cut(s) 691, 833, 868
AjuI GAANNNNNNNTTGG 2 cut(s) 617, 649
AluBI AGCT 5 cut(s) 337, 364, 529, 687, 872
AluI AGCT 5 cut(s) 337, 364, 529, 687, 872
Alw21I GWGCWC 2 cut(s) 339, 531
Alw26I GTCTC 2 cut(s) 166, 566
Aor13HI TCCGGA 1 cut(s) 773
AoxI GGCC 6 cut(s) 3, 582, 587, 600, 750, 758
ApaI GGGCCC 1 cut(s) 762
ApoI RAATTY 1 cut(s) 390
ArsI GACNNNNNNTTYG 2 cut(s) 335, 367
AsiGI ACCGGT 1 cut(s) 475
Asp700I GAANNNNTTC 1 cut(s) 686
Asp718I GGTACC 2 cut(s) 397, 472
AspLEI GCGC 3 cut(s) 299, 319, 436
AspS9I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
AsuC2I CCSGG 2 cut(s) 524, 586
AsuHPI GGTGA 6 cut(s) 16, 46, 88, 127, 298, 820
BaeGI GKGCMC 2 cut(s) 734, 762
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 4 cut(s) 296, 397, 472, 508
BanII GRGCYC 5 cut(s) 339, 531, 550, 762, 781
BbsI GAAGAC 1 cut(s) 295
Bbv12I GWGCWC 2 cut(s) 339, 531
BccI CCATC 1 cut(s) 245
BceAI ACGGC 7 cut(s) 5, 197, 284, 310, 364, 366, 460
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BcnI CCSGG 2 cut(s) 524, 586
BcoDI GTCTC 2 cut(s) 166, 566
BfaI CTAG 1 cut(s) 654
BfmI CTRYAG 1 cut(s) 660
BfoI RGCGCY 1 cut(s) 300
BisI GCNGC 7 cut(s) 75, 243, 255, 258, 315, 600, 750
BlsI GCNGC 7 cut(s) 76, 244, 256, 259, 316, 601, 751
Bme1390I CCNGG 2 cut(s) 524, 586
BmgT120I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
BmiI GGNNCC 7 cut(s) 298, 399, 474, 510, 549, 760, 778
BmrFI CCNGG 2 cut(s) 524, 586
BmsI GCATC 1 cut(s) 628
BpiI GAAGAC 1 cut(s) 295
BpuMI CCSGG 2 cut(s) 524, 586
BsaHI GRCGYC 3 cut(s) 267, 297, 330
BsaJI CCNNGG 2 cut(s) 754, 787
BsaWI WCCGGW 5 cut(s) 469, 475, 502, 550, 773
BsaXI ACNNNNNCTCC 6 cut(s) 137, 167, 191, 221, 484, 514
Bsc4I CCNNNNNNNGG 1 cut(s) 610
Bse118I RCCGGY 1 cut(s) 475
BseAI TCCGGA 1 cut(s) 773
BseDI CCNNGG 2 cut(s) 754, 787
BseGI GGATG 2 cut(s) 274, 643
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMII CTCAG 2 cut(s) 379, 849
BseRI GAGGAG 2 cut(s) 112, 272
BseSI GKGCMC 2 cut(s) 734, 762
BseYI CCCAGC 2 cut(s) 703, 708
Bsh1236I CGCG 2 cut(s) 317, 434
Bsh1285I CGRYCG 1 cut(s) 408
BshFI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BshNI GGYRCC 4 cut(s) 296, 397, 472, 508
BshTI ACCGGT 1 cut(s) 475
BsiEI CGRYCG 1 cut(s) 408
BsiHKAI GWGCWC 2 cut(s) 339, 531
BsiSI CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
BsiWI CGTACG 1 cut(s) 567
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 2 cut(s) 166, 566
BsmBI CGTCTC 2 cut(s) 166, 566
BsmI GAATGC 1 cut(s) 50
BsnI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
Bsp120I GGGCCC 1 cut(s) 758
Bsp1286I GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
Bsp13I TCCGGA 1 cut(s) 773
Bsp143I GATC 1 cut(s) 373
BspANI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BspCNI CTCAG 2 cut(s) 378, 850
BspEI TCCGGA 1 cut(s) 773
BspFNI CGCG 2 cut(s) 317, 434
BspLI GGNNCC 7 cut(s) 298, 399, 474, 510, 549, 760, 778
BspMAI CTGCAG 1 cut(s) 664
BspQI GCTCTTC 1 cut(s) 677
BspT107I GGYRCC 4 cut(s) 296, 397, 472, 508
BsrFI RCCGGY 1 cut(s) 475
BssAI RCCGGY 1 cut(s) 475
BssECI CCNNGG 2 cut(s) 754, 787
BssMI GATC 1 cut(s) 373
BssNI GRCGYC 3 cut(s) 267, 297, 330
BssT1I CCWWGG 1 cut(s) 787
Bst4CI ACNGT 5 cut(s) 46, 139, 160, 310, 827
Bst6I CTCTTC 2 cut(s) 209, 677
BstACI GRCGYC 3 cut(s) 267, 297, 330
BstC8I GCNNGC 2 cut(s) 79, 362
BstDEI CTNAG 3 cut(s) 365, 858, 873
BstDSI CCRYGG 1 cut(s) 754
BstF5I GGATG 2 cut(s) 274, 643
BstFNI CGCG 2 cut(s) 317, 434
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 3 cut(s) 299, 319, 436
BstKTI GATC 1 cut(s) 376
BstMAI GTCTC 2 cut(s) 166, 566
BstMBI GATC 1 cut(s) 373
BstMCI CGRYCG 1 cut(s) 408
BstMWI GCNNNNNNNGC 5 cut(s) 242, 251, 357, 638, 776
BstSCI CCNGG 2 cut(s) 522, 584
BstSFI CTRYAG 1 cut(s) 660
BstSLI GKGCMC 2 cut(s) 734, 762
BstUI CGCG 2 cut(s) 317, 434
BstV2I GAAGAC 1 cut(s) 295
BsuRI GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
BtgI CCRYGG 1 cut(s) 754
BtgZI GCGATG 1 cut(s) 223
BtsCI GGATG 2 cut(s) 274, 643
BtsI GCAGTG 1 cut(s) 657
BtsIMutI CAGTG 1 cut(s) 657
Cac8I GCNNGC 2 cut(s) 79, 362
CfoI GCGC 3 cut(s) 299, 319, 436
Cfr10I RCCGGY 1 cut(s) 475
Cfr13I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
CseI GACGC 1 cut(s) 338
Csp6I GTAC 3 cut(s) 398, 473, 568
CspAI ACCGGT 1 cut(s) 475
CviQI GTAC 3 cut(s) 398, 473, 568
DdeI CTNAG 3 cut(s) 365, 858, 873
DinI GGCGCC 1 cut(s) 298
DpnI GATC 1 cut(s) 375
DpnII GATC 1 cut(s) 373
DrdI GACNNNNNNGTC 1 cut(s) 353
DseDI GACNNNNNNGTC 1 cut(s) 353
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 209, 677
EarI CTCTTC 2 cut(s) 209, 677
EciI GGCGGA 6 cut(s) 51, 74, 154, 199, 241, 462
Ecl136II GAGCTC 2 cut(s) 337, 529
Eco130I CCWWGG 1 cut(s) 787
Eco24I GRGCYC 5 cut(s) 339, 531, 550, 762, 781
Eco53kI GAGCTC 2 cut(s) 337, 529
Eco57I CTGAAG 1 cut(s) 701
EcoICRI GAGCTC 2 cut(s) 337, 529
EcoRI GAATTC 1 cut(s) 390
EcoT14I CCWWGG 1 cut(s) 787
EcoT38I GRGCYC 5 cut(s) 339, 531, 550, 762, 781
EgeI GGCGCC 1 cut(s) 298
EheI GGCGCC 1 cut(s) 298
ErhI CCWWGG 1 cut(s) 787
Esp3I CGTCTC 2 cut(s) 166, 566
FauI CCCGC 2 cut(s) 86, 236
Fnu4HI GCNGC 7 cut(s) 75, 243, 255, 258, 315, 600, 750
FokI GGATG 2 cut(s) 261, 650
FriOI GRGCYC 5 cut(s) 339, 531, 550, 762, 781
Fsp4HI GCNGC 7 cut(s) 75, 243, 255, 258, 315, 600, 750
FspBI CTAG 1 cut(s) 654
GlaI GCGC 3 cut(s) 298, 318, 435
GluI GCNGC 7 cut(s) 75, 243, 255, 258, 315, 600, 750
GsaI CCCAGC 2 cut(s) 707, 712
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 6 cut(s) 5, 584, 589, 602, 752, 760
HapII CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
HgaI GACGC 1 cut(s) 338
HhaI GCGC 3 cut(s) 299, 319, 436
Hin1I GRCGYC 3 cut(s) 267, 297, 330
Hin6I GCGC 3 cut(s) 297, 317, 434
HinP1I GCGC 3 cut(s) 297, 317, 434
HindIII AAGCTT 1 cut(s) 870
HinfI GANTC 2 cut(s) 618, 845
HpaII CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
HphI GGTGA 6 cut(s) 16, 46, 88, 127, 298, 820
Hpy166II GTNNAC 3 cut(s) 142, 430, 651
Hpy188I TCNGA 5 cut(s) 389, 405, 533, 797, 859
Hpy188III TCNNGA 4 cut(s) 371, 622, 721, 774
Hpy8I GTNNAC 3 cut(s) 142, 430, 651
Hpy99I CGWCG 9 cut(s) 50, 218, 269, 272, 314, 347, 350, 381, 446
HpyAV CCTTC 4 cut(s) 201, 229, 234, 256
HpyCH4III ACNGT 5 cut(s) 46, 139, 160, 310, 827
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 1 cut(s) 662
HpyF10VI GCNNNNNNNGC 5 cut(s) 242, 251, 357, 638, 776
HpyF3I CTNAG 3 cut(s) 365, 858, 873
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 3 cut(s) 267, 297, 330
HspAI GCGC 3 cut(s) 297, 317, 434
KasI GGCGCC 1 cut(s) 296
Kpn2I TCCGGA 1 cut(s) 773
KpnI GGTACC 2 cut(s) 401, 476
Kzo9I GATC 1 cut(s) 373
LguI GCTCTTC 1 cut(s) 677
LmnI GCTCC 4 cut(s) 118, 526, 553, 776
LweI GCATC 1 cut(s) 628
MaeI CTAG 1 cut(s) 654
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 3 cut(s) 22, 675, 763
MalI GATC 1 cut(s) 375
MboI GATC 1 cut(s) 373
MboII GAAGA 3 cut(s) 196, 295, 694
MhlI GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 390, 725
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 297
MmeI TCCRAC 2 cut(s) 428, 775
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 773
MroXI GAANNNNTTC 1 cut(s) 686
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 9 cut(s) 470, 476, 503, 512, 523, 551, 585, 590, 774
MspR9I CCNGG 2 cut(s) 524, 586
Mva1269I GAATGC 1 cut(s) 50
MvnI CGCG 2 cut(s) 317, 434
MwoI GCNNNNNNNGC 5 cut(s) 242, 251, 357, 638, 776
NarI GGCGCC 1 cut(s) 297
NciI CCSGG 2 cut(s) 524, 586
NdeII GATC 1 cut(s) 373
NlaIV GGNNCC 7 cut(s) 298, 399, 474, 510, 549, 760, 778
NmeAIII GCCGAG 4 cut(s) 300, 358, 417, 441
NmuCI GTSAC 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 677
PcsI WCGNNNNNNNCGW 3 cut(s) 351, 419, 438
PctI GAATGC 1 cut(s) 50
PdmI GAANNNNTTC 1 cut(s) 686
PfeI GAWTC 2 cut(s) 618, 845
Pfl23II CGTACG 1 cut(s) 567
PflFI GACNNNGTC 1 cut(s) 266
PflMI CCANNNNNTGG 1 cut(s) 610
PfoI TCCNGGA 1 cut(s) 522
PinAI ACCGGT 1 cut(s) 475
PkrI GCNGC 7 cut(s) 76, 244, 256, 259, 316, 601, 751
PluTI GGCGCC 1 cut(s) 300
Psp124BI GAGCTC 2 cut(s) 339, 531
PspFI CCCAGC 2 cut(s) 703, 708
PspLI CGTACG 1 cut(s) 567
PspN4I GGNNCC 7 cut(s) 298, 399, 474, 510, 549, 760, 778
PspOMI GGGCCC 1 cut(s) 758
PspPI GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
PstI CTGCAG 1 cut(s) 664
PsyI GACNNNGTC 1 cut(s) 266
RsaI GTAC 3 cut(s) 399, 474, 569
RsaNI GTAC 3 cut(s) 398, 473, 568
SacI GAGCTC 2 cut(s) 339, 531
SapI GCTCTTC 1 cut(s) 677
SatI GCNGC 7 cut(s) 75, 243, 255, 258, 315, 600, 750
Sau3AI GATC 1 cut(s) 373
Sau96I GGNCC 6 cut(s) 582, 587, 601, 751, 758, 759
ScrFI CCNGG 2 cut(s) 524, 586
SduI GDGCHC 6 cut(s) 339, 531, 550, 734, 762, 781
SfaNI GCATC 1 cut(s) 628
SfcI CTRYAG 1 cut(s) 660
SfoI GGCGCC 1 cut(s) 298
Sse9I AATT 2 cut(s) 390, 725
SspDI GGCGCC 1 cut(s) 296
SspMI CTAG 1 cut(s) 654
SstI GAGCTC 2 cut(s) 339, 531
StyD4I CCNGG 2 cut(s) 522, 584
StyI CCWWGG 1 cut(s) 787
TaaI ACNGT 5 cut(s) 46, 139, 160, 310, 827
TaiI ACGT 1 cut(s) 270
TaqI TCGA 3 cut(s) 342, 370, 822
TaqII GACCGA 1 cut(s) 509
TasI AATT 2 cut(s) 390, 725
TauI GCSGC 7 cut(s) 77, 245, 257, 260, 317, 602, 752
TfiI GAWTC 2 cut(s) 618, 845
TscAI CASTG 1 cut(s) 664
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspRI CASTG 1 cut(s) 664
Tth111I GACNNNGTC 1 cut(s) 266
Van91I CCANNNNNTGG 1 cut(s) 610
XapI RAATTY 1 cut(s) 390
XmnI GAANNNNTTC 1 cut(s) 686
XspI CTAG 1 cut(s) 654
ZraI GACGTC 1 cut(s) 268
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.