pycom15g14150

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0016720
Physical Location & Seq
Forward (+)
9642822 .. 9643691
870 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g14150.1

Sequence Viewer

Length: 870 bp
ATGGCCGCCGCGCATGGCAGTGGCAGCATCCCCACCGTCGCATTCACCACCCCTCCAAACTACGGCGCCAGGCTGGCCCACCTCCTCACCCTCACAGGCTTCAAACCCCTCTCCGCCCCCACACTCATCGTTCAGCCCACGCCGTCCACCGTCGCCGCCCTCAAACCCCACCTCTCACCCCCTTCCCTCGACCTCTTCTCCGCCGTCGCCTTCCCTTCACGCACAGCCGTAGCCGCGTTCTCATCCGCCGCCGCAGACGTCCCCCACCCTCTCCTCTCCCCGCACGGGGACGTATTCACCGTCGCCGCGCTGGGAAAGGACTCCGAGCTCATAGACGACAGTTTCGTCCAGAAGCTCTGTCCCAATCCTGACCGACTCAGAATTTTAGTGCCTTTGACTGCGACCCCGAACGCATTAGTCCAATCGCTCGGAGACGGGCGCGGTCGAAGGGTGTTGTGTCCAGTCCCGCTAGTGGTGGGACTGGTCGAGCCTCCCGTGGTCCCACACTTTCTTCGGGACTTGGAGGCCAAGCATTGGGTTCCAGTTAGGGTAAACGCGTACGAGACGCGATGGGCCGGCCCGGGCTGTGCCGAGAAAGTGGTGGAGAAGATCGAAGATGGAGCGTTGGATGCGATTGTGTTTACGAGCACAGCGGAGGTGGAGGGGTTGCTGAAGAGCTTCAAAGAGTTTGGGCTGGACTGGGAGACTGCCAAGAGGACGTGCCCCAAAATGCTGGTGGCGGCCCATGGCCCCATCACGGCAGCCGGGGCCCAGATGCTTGGAGTTGGAGTTGATTTGGTGAGTTCTCAGTTTGGTAGCTTTCAAGGAGTTGTTGATGCTTTGCACACTGAGATTTTGAGATTGAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

290

Amino Acids

30.44

Weight (kDa)

6.3

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 24 - 271 1.3e-17 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 344
AatII GACGTC 1 cut(s) 261
AccB1I GGYRCC 1 cut(s) 65
AccB7I CCANNNNNTGG 1 cut(s) 534
AccII CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 381
AcuI CTGAAG 1 cut(s) 692
AcyI GRCGYC 2 cut(s) 66, 258
AfaI GTAC 1 cut(s) 560
AfiI CCNNNNNNNGG 6 cut(s) 62, 285, 286, 472, 534, 757
AflIII ACRYGT 1 cut(s) 555
AgsI TTSAA 3 cut(s) 103, 682, 824
AjiI CACGTC 1 cut(s) 720
AjnI CCWGG 1 cut(s) 68
AluBI AGCT 4 cut(s) 328, 355, 678, 819
AluI AGCT 4 cut(s) 328, 355, 678, 819
Alw21I GWGCWC 2 cut(s) 330, 650
Alw26I GTCTC 3 cut(s) 426, 557, 698
Ama87I CYCGRG 1 cut(s) 580
AoxI GGCC 8 cut(s) 3, 75, 525, 573, 577, 741, 748, 768
ApaI GGGCCC 1 cut(s) 772
ApeKI GCWGC 2 cut(s) 24, 761
ApoI RAATTY 1 cut(s) 381
ArsI GACNNNNNNTTYG 2 cut(s) 326, 358
Asp700I GAANNNNTTC 1 cut(s) 677
AspLEI GCGC 4 cut(s) 13, 68, 310, 441
AspS9I GGNCC 8 cut(s) 76, 499, 573, 578, 742, 749, 768, 769
AsuC2I CCSGG 3 cut(s) 581, 582, 766
AsuHPI GGTGA 5 cut(s) 37, 79, 168, 289, 811
AvaI CYCGRG 1 cut(s) 580
AvaII GGWCC 1 cut(s) 499
BaeGI GKGCMC 2 cut(s) 725, 772
BanI GGYRCC 1 cut(s) 65
BanII GRGCYC 2 cut(s) 330, 772
Bbv12I GWGCWC 2 cut(s) 330, 650
BbvI GCAGC 2 cut(s) 36, 773
BccI CCATC 3 cut(s) 564, 611, 761
BceAI ACGGC 5 cut(s) 79, 127, 188, 212, 774
BciT130I CCWGG 1 cut(s) 70
BcnI CCSGG 3 cut(s) 581, 582, 766
BcoDI GTCTC 3 cut(s) 426, 557, 698
BfaI CTAG 1 cut(s) 470
BfoI RGCGCY 1 cut(s) 69
BglI GCCNNNNNGGC 1 cut(s) 74
Bme1390I CCNGG 4 cut(s) 70, 581, 582, 766
Bme18I GGWCC 1 cut(s) 499
BmeT110I CYCGRG 1 cut(s) 580
BmgBI CACGTC 1 cut(s) 720
BmgT120I GGNCC 8 cut(s) 76, 499, 573, 578, 742, 749, 768, 769
BmiI GGNNCC 6 cut(s) 67, 501, 540, 751, 769, 770
BmrFI CCNGG 4 cut(s) 70, 581, 582, 766
BmrI ACTGGG 1 cut(s) 709
BmsI GCATC 4 cut(s) 36, 619, 765, 826
BmuI ACTGGG 1 cut(s) 709
BpuMI CCSGG 3 cut(s) 581, 582, 766
BsaHI GRCGYC 2 cut(s) 66, 258
BsaJI CCNNGG 4 cut(s) 495, 580, 745, 765
BsaXI ACNNNNNCTCC 6 cut(s) 37, 67, 182, 212, 774, 804
Bsc4I CCNNNNNNNGG 6 cut(s) 62, 285, 286, 472, 534, 757
Bse118I RCCGGY 1 cut(s) 575
Bse1I ACTGG 4 cut(s) 461, 486, 542, 704
BseBI CCWGG 1 cut(s) 70
BseDI CCNNGG 4 cut(s) 495, 580, 745, 765
BseGI GGATG 3 cut(s) 27, 242, 634
BseLI CCNNNNNNNGG 6 cut(s) 62, 285, 286, 472, 534, 757
BseMII CTCAG 3 cut(s) 391, 821, 840
BseNI ACTGG 4 cut(s) 461, 486, 542, 704
BseRI GAGGAG 2 cut(s) 74, 263
BseSI GKGCMC 2 cut(s) 725, 772
BseXI GCAGC 2 cut(s) 36, 773
BseYI CCCAGC 1 cut(s) 310
Bsh1236I CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
Bsh1285I CGRYCG 1 cut(s) 445
BshFI GGCC 8 cut(s) 5, 77, 527, 575, 579, 743, 750, 770
BshNI GGYRCC 1 cut(s) 65
BsiEI CGRYCG 1 cut(s) 445
BsiHKAI GWGCWC 2 cut(s) 330, 650
BsiHKCI CYCGRG 1 cut(s) 580
BsiSI CCGG 3 cut(s) 576, 581, 765
BsiWI CGTACG 1 cut(s) 558
BslFI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
BslI CCNNNNNNNGG 6 cut(s) 62, 285, 286, 472, 534, 757
BsmAI GTCTC 3 cut(s) 426, 557, 698
BsmBI CGTCTC 2 cut(s) 426, 557
BsmFI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
BsmI GAATGC 1 cut(s) 41
BsnI GGCC 8 cut(s) 5, 77, 527, 575, 579, 743, 750, 770
BsoBI CYCGRG 1 cut(s) 580
Bsp120I GGGCCC 1 cut(s) 768
Bsp1286I GDGCHC 4 cut(s) 330, 650, 725, 772
Bsp143I GATC 1 cut(s) 609
Bsp19I CCATGG 1 cut(s) 745
BspANI GGCC 8 cut(s) 5, 77, 527, 575, 579, 743, 750, 770
BspCNI CTCAG 3 cut(s) 390, 820, 841
BspFNI CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
BspLI GGNNCC 6 cut(s) 67, 501, 540, 751, 769, 770
BspQI GCTCTTC 1 cut(s) 668
BspT107I GGYRCC 1 cut(s) 65
BsrFI RCCGGY 1 cut(s) 575
BsrI ACTGG 4 cut(s) 461, 486, 542, 704
BssAI RCCGGY 1 cut(s) 575
BssECI CCNNGG 4 cut(s) 495, 580, 745, 765
BssMI GATC 1 cut(s) 609
BssNI GRCGYC 2 cut(s) 66, 258
BssT1I CCWWGG 1 cut(s) 745
Bst2UI CCWGG 1 cut(s) 70
Bst4CI ACNGT 4 cut(s) 37, 151, 301, 341
Bst6I CTCTTC 2 cut(s) 200, 668
BstACI GRCGYC 2 cut(s) 66, 258
BstC8I GCNNGC 2 cut(s) 75, 577
BstDEI CTNAG 3 cut(s) 377, 807, 849
BstDSI CCRYGG 2 cut(s) 495, 745
BstF5I GGATG 3 cut(s) 27, 242, 634
BstFNI CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
BstH2I RGCGCY 1 cut(s) 69
BstHHI GCGC 4 cut(s) 13, 68, 310, 441
BstKTI GATC 1 cut(s) 612
BstMAI GTCTC 3 cut(s) 426, 557, 698
BstMBI GATC 1 cut(s) 609
BstMCI CGRYCG 1 cut(s) 445
BstMWI GCNNNNNNNGC 5 cut(s) 24, 74, 233, 629, 767
BstNI CCWGG 1 cut(s) 70
BstSCI CCNGG 4 cut(s) 68, 579, 580, 764
BstSLI GKGCMC 2 cut(s) 725, 772
BstUI CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
BstV1I GCAGC 2 cut(s) 36, 773
BstXI CCANNNNNNTGG 2 cut(s) 733, 779
BsuRI GGCC 8 cut(s) 5, 77, 527, 575, 579, 743, 750, 770
BtgI CCRYGG 2 cut(s) 495, 745
BtgZI GCGATG 1 cut(s) 583
BtrI CACGTC 1 cut(s) 720
BtsCI GGATG 3 cut(s) 27, 242, 634
BtsI GCAGTG 1 cut(s) 25
BtsIMutI CAGTG 2 cut(s) 25, 846
Cac8I GCNNGC 2 cut(s) 75, 577
CfoI GCGC 4 cut(s) 13, 68, 310, 441
Cfr10I RCCGGY 1 cut(s) 575
Cfr13I GGNCC 8 cut(s) 76, 499, 573, 578, 742, 749, 768, 769
Cfr9I CCCGGG 1 cut(s) 580
CseI GACGC 1 cut(s) 574
Csp6I GTAC 1 cut(s) 559
CviAII CATG 2 cut(s) 14, 746
CviQI GTAC 1 cut(s) 559
DdeI CTNAG 3 cut(s) 377, 807, 849
DinI GGCGCC 1 cut(s) 67
DpnI GATC 1 cut(s) 611
DpnII GATC 1 cut(s) 609
DrdI GACNNNNNNGTC 1 cut(s) 344
DseDI GACNNNNNNGTC 1 cut(s) 344
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 200, 668
EarI CTCTTC 2 cut(s) 200, 668
EciI GGCGGA 3 cut(s) 103, 190, 235
Ecl136II GAGCTC 1 cut(s) 328
Eco130I CCWWGG 1 cut(s) 745
Eco24I GRGCYC 2 cut(s) 330, 772
Eco47I GGWCC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 328
Eco57I CTGAAG 1 cut(s) 692
Eco88I CYCGRG 1 cut(s) 580
EcoICRI GAGCTC 1 cut(s) 328
EcoO109I RGGNCCY 1 cut(s) 768
EcoRII CCWGG 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 745
EcoT38I GRGCYC 2 cut(s) 330, 772
EgeI GGCGCC 1 cut(s) 67
EheI GGCGCC 1 cut(s) 67
ErhI CCWWGG 1 cut(s) 745
Esp3I CGTCTC 2 cut(s) 426, 557
FaeI CATG 2 cut(s) 17, 749
FaiI YATR 3 cut(s) 15, 332, 747
FaqI GGGAC 7 cut(s) 245, 302, 345, 449, 485, 492, 530
FatI CATG 2 cut(s) 13, 745
FauI CCCGC 2 cut(s) 288, 474
FokI GGATG 3 cut(s) 14, 229, 641
FriOI GRGCYC 2 cut(s) 330, 772
FseI GGCCGGCC 1 cut(s) 579
FspBI CTAG 1 cut(s) 470
GlaI GCGC 4 cut(s) 12, 67, 309, 440
GsaI CCCAGC 1 cut(s) 314
HaeII RGCGCY 1 cut(s) 69
HaeIII GGCC 8 cut(s) 5, 77, 527, 575, 579, 743, 750, 770
HapII CCGG 3 cut(s) 576, 581, 765
HgaI GACGC 1 cut(s) 574
HhaI GCGC 4 cut(s) 13, 68, 310, 441
Hin1I GRCGYC 2 cut(s) 66, 258
Hin1II CATG 2 cut(s) 17, 749
Hin6I GCGC 4 cut(s) 11, 66, 308, 439
HinP1I GCGC 4 cut(s) 11, 66, 308, 439
HinfI GANTC 2 cut(s) 320, 375
HpaII CCGG 3 cut(s) 576, 581, 765
HphI GGTGA 5 cut(s) 37, 79, 168, 289, 811
Hpy166II GTNNAC 3 cut(s) 147, 553, 642
Hpy188I TCNGA 3 cut(s) 325, 380, 431
Hpy188III TCNNGA 3 cut(s) 349, 368, 515
Hpy8I GTNNAC 3 cut(s) 147, 553, 642
Hpy99I CGWCG 4 cut(s) 41, 155, 209, 305
HpyAV CCTTC 4 cut(s) 192, 220, 225, 441
HpyCH4III ACNGT 4 cut(s) 37, 151, 301, 341
HpyCH4IV ACGT 3 cut(s) 258, 291, 719
HpyCH4V TGCA 1 cut(s) 844
HpyF10VI GCNNNNNNNGC 5 cut(s) 24, 74, 233, 629, 767
HpyF3I CTNAG 3 cut(s) 377, 807, 849
HpySE526I ACGT 3 cut(s) 258, 291, 719
Hsp92I GRCGYC 2 cut(s) 66, 258
Hsp92II CATG 2 cut(s) 17, 749
HspAI GCGC 4 cut(s) 11, 66, 308, 439
KasI GGCGCC 1 cut(s) 65
KroI GCCGGC 1 cut(s) 575
KroNI GCCGGC 1 cut(s) 577
Kzo9I GATC 1 cut(s) 609
LguI GCTCTTC 1 cut(s) 668
LmnI GCTCC 1 cut(s) 620
Lsp1109I GCAGC 2 cut(s) 36, 773
LweI GCATC 4 cut(s) 36, 619, 765, 826
MaeI CTAG 1 cut(s) 470
MaeII ACGT 3 cut(s) 258, 291, 719
MalI GATC 1 cut(s) 611
MboI GATC 1 cut(s) 609
MboII GAAGA 5 cut(s) 187, 503, 619, 626, 685
MhlI GDGCHC 4 cut(s) 330, 650, 725, 772
MluCI AATT 1 cut(s) 381
MluI ACGCGT 1 cut(s) 555
Mly113I GGCGCC 1 cut(s) 66
MlyI GAGTC 2 cut(s) 314, 369
MmeI TCCRAC 2 cut(s) 606, 766
MroNI GCCGGC 1 cut(s) 575
MroXI GAANNNNTTC 1 cut(s) 677
MseI TTAA 1 cut(s) 868
MslI CAYNNNNRTG 1 cut(s) 18
MspA1I CMGCKG 1 cut(s) 653
MspI CCGG 3 cut(s) 576, 581, 765
MspR9I CCNGG 4 cut(s) 70, 581, 582, 766
Mva1269I GAATGC 1 cut(s) 41
MvaI CCWGG 1 cut(s) 70
MvnI CGCG 6 cut(s) 11, 236, 308, 441, 557, 568
MwoI GCNNNNNNNGC 5 cut(s) 24, 74, 233, 629, 767
NaeI GCCGGC 1 cut(s) 577
NarI GGCGCC 1 cut(s) 66
NciI CCSGG 3 cut(s) 581, 582, 766
NcoI CCATGG 1 cut(s) 745
NdeII GATC 1 cut(s) 609
NgoMIV GCCGGC 1 cut(s) 575
NlaIII CATG 2 cut(s) 17, 749
NlaIV GGNNCC 6 cut(s) 67, 501, 540, 751, 769, 770
NmeAIII GCCGAG 1 cut(s) 616
PciSI GCTCTTC 1 cut(s) 668
PcsI WCGNNNNNNNCGW 3 cut(s) 297, 342, 492
PctI GAATGC 1 cut(s) 41
PdiI GCCGGC 1 cut(s) 577
PdmI GAANNNNTTC 1 cut(s) 677
Pfl23II CGTACG 1 cut(s) 558
PflMI CCANNNNNTGG 1 cut(s) 534
PleI GAGTC 2 cut(s) 314, 369
PluTI GGCGCC 1 cut(s) 69
PpsI GAGTC 2 cut(s) 314, 369
Psp124BI GAGCTC 1 cut(s) 330
Psp6I CCWGG 1 cut(s) 68
PspFI CCCAGC 1 cut(s) 310
PspGI CCWGG 1 cut(s) 68
PspLI CGTACG 1 cut(s) 558
PspN4I GGNNCC 6 cut(s) 67, 501, 540, 751, 769, 770
PspOMI GGGCCC 1 cut(s) 768
PspPI GGNCC 8 cut(s) 76, 499, 573, 578, 742, 749, 768, 769
RigI GGCCGGCC 1 cut(s) 579
RsaI GTAC 1 cut(s) 560
RsaNI GTAC 1 cut(s) 559
RseI CAYNNNNRTG 1 cut(s) 18
SacI GAGCTC 1 cut(s) 330
SapI GCTCTTC 1 cut(s) 668
SaqAI TTAA 1 cut(s) 868
Sau3AI GATC 1 cut(s) 609
Sau96I GGNCC 8 cut(s) 76, 499, 573, 578, 742, 749, 768, 769
SchI GAGTC 2 cut(s) 314, 369
ScrFI CCNGG 4 cut(s) 70, 581, 582, 766
SduI GDGCHC 4 cut(s) 330, 650, 725, 772
SfaNI GCATC 4 cut(s) 36, 619, 765, 826
SfoI GGCGCC 1 cut(s) 67
SinI GGWCC 1 cut(s) 499
SmaI CCCGGG 1 cut(s) 582
SmiMI CAYNNNNRTG 1 cut(s) 18
SrfI GCCCGGGC 1 cut(s) 582
Sse9I AATT 1 cut(s) 381
SspDI GGCGCC 1 cut(s) 65
SspMI CTAG 1 cut(s) 470
SstI GAGCTC 1 cut(s) 330
StyD4I CCNGG 4 cut(s) 68, 579, 580, 764
StyI CCWWGG 1 cut(s) 745
TaaI ACNGT 4 cut(s) 37, 151, 301, 341
TaiI ACGT 3 cut(s) 261, 294, 722
TaqI TCGA 4 cut(s) 189, 445, 486, 612
TaqII GACCGA 1 cut(s) 387
TasI AATT 1 cut(s) 381
TauI GCSGC 8 cut(s) 8, 11, 158, 236, 251, 254, 308, 743
Tru1I TTAA 1 cut(s) 868
Tru9I TTAA 1 cut(s) 868
TscAI CASTG 2 cut(s) 25, 853
TseI GCWGC 2 cut(s) 24, 761
TspMI CCCGGG 1 cut(s) 580
TspRI CASTG 2 cut(s) 25, 853
Van91I CCANNNNNTGG 1 cut(s) 534
VpaK11BI GGWCC 1 cut(s) 499
XapI RAATTY 1 cut(s) 381
XcmI CCANNNNNNNNNTGG 1 cut(s) 733
XmaI CCCGGG 1 cut(s) 580
XmnI GAANNNNTTC 1 cut(s) 677
XspI CTAG 1 cut(s) 470
ZraI GACGTC 1 cut(s) 259
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.