Rh2BG017700

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016720
Physical Location & Seq
Forward (+)
1291394 .. 1292263
870 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG017700.1

Sequence Viewer

Length: 870 bp
ATGGCCACACCAAACACCGCCGTCACCACCACCCCCACCGTCGCATTCACCACTCCGCCAATCTACGCCGCCCGCCTCCGCCACCTCCTCACCCTCAGCAACTTCACTCCCCTCTCCTCTCCCACACTCACCGTCCACCACACCCCCTCCACCGTCTCCGCCCTCAAACCCCACCTCTCGCCTCCCTCCCTAAACCTCTTCTCCGCCGTCGCCTTCCCTTCCCGCACCGCCATCGCCGCCTTCTCCGCCGCCTCAGACGACGTCGCTCACCCTCTCCTCTCCCCAAACGGCGCCGTCTTCACCGTCGCCGCGCTCGGCAAGGACGCCGAGCTCATCGACGACGGCTTCGTCCTCAAGCTCAGTCGAGATCGTCGCCGTGTCAGAATTCTGGTCCCTCCGACCGCCACGCCGAGCGCGTTGGTTCACGCGCTCGGCGACGGCGGCGGCAGGAGGGTGCTGTGTCCGGTACCGGTGGTGTTTGGACTGACTGAGCCTCCGGTGGTGCCGGATTTTCTCCGGGAGCTCGGTGAAAGCGGTTGGGCTCCGGTTCGGGTCAATGCGTACGAGACGAGGTGGGCCGGGCCGGAGTGTGCGGCCCAAGTGGTGGAGAGAATCAAGAACGGGGCTTTGGATGCGATTGTGTTTACTAGCACTGCGGAGGTGGAGGGGCTACTGAAGAGCTTCAAGGAGTTTGGGCTGGGCTGGGAGAGTGTCAAGAATTACTGCCCGAAACTGCTCGTGGCGGCCCACGGGCCCGTTACTGCCGCCGGAGCCCGAACGCTTGGTGTCGGAGTAGATTTGGTGAGTTCTAAGTTCGATAGTTTTCAAGGTGTTGTTGATTCTTTGCGTTCTGAGATTTTCAAAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

289

Amino Acids

30.51

Weight (kDa)

8.47

Isoelectric Point (pI)

41.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HEM4 PF02602 30 - 273 3.8e-17 Uroporphyrinogen-III synthase HemD
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 347
AatII GACGTC 1 cut(s) 264
Acc65I GGTACC 1 cut(s) 466
AccB1I GGYRCC 3 cut(s) 290, 466, 502
AccB7I CCANNNNNTGG 1 cut(s) 604
AccII CGCG 3 cut(s) 311, 416, 428
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 384
AcuI CTGAAG 1 cut(s) 695
AcyI GRCGYC 3 cut(s) 261, 291, 324
AfaI GTAC 2 cut(s) 468, 563
AfiI CCNNNNNNNGG 1 cut(s) 604
AgeI ACCGGT 1 cut(s) 469
AgsI TTSAA 3 cut(s) 685, 827, 862
AjuI GAANNNNNNNTTGG 2 cut(s) 611, 643
AluBI AGCT 5 cut(s) 331, 358, 523, 681, 866
AluI AGCT 5 cut(s) 331, 358, 523, 681, 866
Alw21I GWGCWC 2 cut(s) 333, 525
Alw26I GTCTC 2 cut(s) 160, 560
AoxI GGCC 6 cut(s) 3, 576, 581, 594, 744, 752
ApaI GGGCCC 1 cut(s) 756
ApoI RAATTY 1 cut(s) 384
ArsI GACNNNNNNTTYG 2 cut(s) 329, 361
AsiGI ACCGGT 1 cut(s) 469
Asp700I GAANNNNTTC 1 cut(s) 680
Asp718I GGTACC 1 cut(s) 466
AspLEI GCGC 4 cut(s) 293, 313, 416, 430
AspS9I GGNCC 7 cut(s) 391, 576, 581, 595, 745, 752, 753
AsuC2I CCSGG 2 cut(s) 518, 580
AsuHPI GGTGA 8 cut(s) 16, 40, 82, 121, 260, 292, 539, 814
AvaII GGWCC 1 cut(s) 391
BaeGI GKGCMC 1 cut(s) 756
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 290, 466, 502
BanII GRGCYC 5 cut(s) 333, 525, 544, 756, 775
BauI CACGAG 1 cut(s) 737
BbsI GAAGAC 1 cut(s) 289
Bbv12I GWGCWC 2 cut(s) 333, 525
BbvCI CCTCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 239
BceAI ACGGC 7 cut(s) 5, 191, 278, 304, 358, 360, 454
BcgI CGANNNNNNTGC 2 cut(s) 214, 248
BcnI CCSGG 2 cut(s) 518, 580
BcoDI GTCTC 2 cut(s) 160, 560
BfaI CTAG 1 cut(s) 648
BfoI RGCGCY 1 cut(s) 294
BisI GCNGC 9 cut(s) 69, 237, 249, 309, 442, 445, 594, 744, 765
BlsI GCNGC 9 cut(s) 70, 238, 250, 310, 443, 446, 595, 745, 766
Bme1390I CCNGG 2 cut(s) 518, 580
Bme18I GGWCC 1 cut(s) 391
BmgT120I GGNCC 7 cut(s) 391, 576, 581, 595, 745, 752, 753
BmiI GGNNCC 7 cut(s) 292, 393, 468, 504, 543, 754, 772
BmrFI CCNGG 2 cut(s) 518, 580
BmsI GCATC 1 cut(s) 622
BpiI GAAGAC 1 cut(s) 289
Bpu10I CCTNAGC 1 cut(s) 95
BpuEI CTTGAG 1 cut(s) 338
BpuMI CCSGG 2 cut(s) 518, 580
BsaHI GRCGYC 3 cut(s) 261, 291, 324
BsaJI CCNNGG 1 cut(s) 748
BsaWI WCCGGW 4 cut(s) 463, 469, 496, 544
BsaXI ACNNNNNCTCC 6 cut(s) 131, 161, 185, 215, 478, 508
Bsc4I CCNNNNNNNGG 1 cut(s) 604
Bse118I RCCGGY 1 cut(s) 469
BseDI CCNNGG 1 cut(s) 748
BseGI GGATG 1 cut(s) 637
BseLI CCNNNNNNNGG 1 cut(s) 604
BseMII CTCAG 5 cut(s) 109, 267, 373, 480, 843
BseRI GAGGAG 3 cut(s) 77, 106, 266
BseSI GKGCMC 1 cut(s) 756
BseYI CCCAGC 2 cut(s) 697, 702
Bsh1236I CGCG 3 cut(s) 311, 416, 428
Bsh1285I CGRYCG 1 cut(s) 402
BshFI GGCC 6 cut(s) 5, 578, 583, 596, 746, 754
BshNI GGYRCC 3 cut(s) 290, 466, 502
BshTI ACCGGT 1 cut(s) 469
BsiEI CGRYCG 1 cut(s) 402
BsiHKAI GWGCWC 2 cut(s) 333, 525
BsiSI CCGG 9 cut(s) 464, 470, 497, 506, 517, 545, 579, 584, 768
BsiWI CGTACG 1 cut(s) 561
BslFI GGGAC 1 cut(s) 377
BslI CCNNNNNNNGG 1 cut(s) 604
BsmAI GTCTC 2 cut(s) 160, 560
BsmBI CGTCTC 2 cut(s) 160, 560
BsmFI GGGAC 1 cut(s) 377
BsmI GAATGC 1 cut(s) 44
BsnI GGCC 6 cut(s) 5, 578, 583, 596, 746, 754
Bsp120I GGGCCC 1 cut(s) 752
Bsp1286I GDGCHC 5 cut(s) 333, 525, 544, 756, 775
Bsp143I GATC 1 cut(s) 367
BspANI GGCC 6 cut(s) 5, 578, 583, 596, 746, 754
BspCNI CTCAG 5 cut(s) 108, 266, 372, 481, 844
BspFNI CGCG 3 cut(s) 311, 416, 428
BspLI GGNNCC 7 cut(s) 292, 393, 468, 504, 543, 754, 772
BspQI GCTCTTC 1 cut(s) 671
BspT107I GGYRCC 3 cut(s) 290, 466, 502
BsrFI RCCGGY 1 cut(s) 469
BssAI RCCGGY 1 cut(s) 469
BssECI CCNNGG 1 cut(s) 748
BssMI GATC 1 cut(s) 367
BssNI GRCGYC 3 cut(s) 261, 291, 324
BssSI CACGAG 1 cut(s) 737
Bst2BI CACGAG 1 cut(s) 737
Bst4CI ACNGT 4 cut(s) 40, 133, 154, 304
Bst6I CTCTTC 2 cut(s) 203, 671
BstACI GRCGYC 3 cut(s) 261, 291, 324
BstC8I GCNNGC 1 cut(s) 73
BstDEI CTNAG 7 cut(s) 95, 253, 359, 489, 810, 852, 867
BstDSI CCRYGG 1 cut(s) 748
BstF5I GGATG 1 cut(s) 637
BstFNI CGCG 3 cut(s) 311, 416, 428
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 4 cut(s) 293, 313, 416, 430
BstKTI GATC 1 cut(s) 370
BstMAI GTCTC 2 cut(s) 160, 560
BstMBI GATC 1 cut(s) 367
BstMCI CGRYCG 1 cut(s) 402
BstMWI GCNNNNNNNGC 5 cut(s) 236, 245, 441, 632, 770
BstSCI CCNGG 2 cut(s) 516, 578
BstSLI GKGCMC 1 cut(s) 756
BstUI CGCG 3 cut(s) 311, 416, 428
BstV2I GAAGAC 1 cut(s) 289
BsuRI GGCC 6 cut(s) 5, 578, 583, 596, 746, 754
BtgI CCRYGG 1 cut(s) 748
BtgZI GCGATG 1 cut(s) 217
BtsCI GGATG 1 cut(s) 637
BtsI GCAGTG 1 cut(s) 651
BtsIMutI CAGTG 1 cut(s) 651
Cac8I GCNNGC 1 cut(s) 73
CfoI GCGC 4 cut(s) 293, 313, 416, 430
Cfr10I RCCGGY 1 cut(s) 469
Cfr13I GGNCC 7 cut(s) 391, 576, 581, 595, 745, 752, 753
CseI GACGC 1 cut(s) 332
Csp6I GTAC 2 cut(s) 467, 562
CspAI ACCGGT 1 cut(s) 469
CviQI GTAC 2 cut(s) 467, 562
DdeI CTNAG 7 cut(s) 95, 253, 359, 489, 810, 852, 867
DinI GGCGCC 1 cut(s) 292
DpnI GATC 1 cut(s) 369
DpnII GATC 1 cut(s) 367
DrdI GACNNNNNNGTC 1 cut(s) 347
DseDI GACNNNNNNGTC 1 cut(s) 347
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 203, 671
EarI CTCTTC 2 cut(s) 203, 671
EciI GGCGGA 5 cut(s) 45, 68, 148, 193, 235
Ecl136II GAGCTC 2 cut(s) 331, 523
Eco24I GRGCYC 5 cut(s) 333, 525, 544, 756, 775
Eco47I GGWCC 1 cut(s) 391
Eco53kI GAGCTC 2 cut(s) 331, 523
Eco57I CTGAAG 1 cut(s) 695
EcoICRI GAGCTC 2 cut(s) 331, 523
EcoRI GAATTC 1 cut(s) 384
EcoT38I GRGCYC 5 cut(s) 333, 525, 544, 756, 775
EgeI GGCGCC 1 cut(s) 292
EheI GGCGCC 1 cut(s) 292
Esp3I CGTCTC 2 cut(s) 160, 560
FaqI GGGAC 1 cut(s) 377
FauI CCCGC 2 cut(s) 80, 230
Fnu4HI GCNGC 9 cut(s) 69, 237, 249, 309, 442, 445, 594, 744, 765
FokI GGATG 1 cut(s) 644
FriOI GRGCYC 5 cut(s) 333, 525, 544, 756, 775
Fsp4HI GCNGC 9 cut(s) 69, 237, 249, 309, 442, 445, 594, 744, 765
FspBI CTAG 1 cut(s) 648
GlaI GCGC 4 cut(s) 292, 312, 415, 429
GluI GCNGC 9 cut(s) 69, 237, 249, 309, 442, 445, 594, 744, 765
GsaI CCCAGC 2 cut(s) 701, 706
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 6 cut(s) 5, 578, 583, 596, 746, 754
HapII CCGG 9 cut(s) 464, 470, 497, 506, 517, 545, 579, 584, 768
HgaI GACGC 1 cut(s) 332
HhaI GCGC 4 cut(s) 293, 313, 416, 430
Hin1I GRCGYC 3 cut(s) 261, 291, 324
Hin6I GCGC 4 cut(s) 291, 311, 414, 428
HinP1I GCGC 4 cut(s) 291, 311, 414, 428
HindIII AAGCTT 1 cut(s) 864
HinfI GANTC 2 cut(s) 612, 839
HpaII CCGG 9 cut(s) 464, 470, 497, 506, 517, 545, 579, 584, 768
HphI GGTGA 8 cut(s) 16, 40, 82, 121, 260, 292, 539, 814
Hpy166II GTNNAC 3 cut(s) 136, 424, 645
Hpy188I TCNGA 5 cut(s) 256, 383, 399, 791, 853
Hpy188III TCNNGA 3 cut(s) 365, 616, 715
Hpy8I GTNNAC 3 cut(s) 136, 424, 645
Hpy99I CGWCG 9 cut(s) 44, 212, 263, 266, 308, 341, 344, 375, 440
HpyAV CCTTC 3 cut(s) 223, 228, 250
HpyCH4III ACNGT 4 cut(s) 40, 133, 154, 304
HpyCH4IV ACGT 1 cut(s) 261
HpyF10VI GCNNNNNNNGC 5 cut(s) 236, 245, 441, 632, 770
HpyF3I CTNAG 7 cut(s) 95, 253, 359, 489, 810, 852, 867
HpySE526I ACGT 1 cut(s) 261
Hsp92I GRCGYC 3 cut(s) 261, 291, 324
HspAI GCGC 4 cut(s) 291, 311, 414, 428
KasI GGCGCC 1 cut(s) 290
KpnI GGTACC 1 cut(s) 470
Kzo9I GATC 1 cut(s) 367
LguI GCTCTTC 1 cut(s) 671
LmnI GCTCC 3 cut(s) 520, 547, 770
LweI GCATC 1 cut(s) 622
MaeI CTAG 1 cut(s) 648
MaeII ACGT 1 cut(s) 261
MaeIII GTNAC 2 cut(s) 22, 757
MalI GATC 1 cut(s) 369
MboI GATC 1 cut(s) 367
MboII GAAGA 3 cut(s) 190, 289, 688
MhlI GDGCHC 5 cut(s) 333, 525, 544, 756, 775
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 2 cut(s) 384, 718
MluNI TGGCCA 1 cut(s) 5
Mly113I GGCGCC 1 cut(s) 291
MmeI TCCRAC 2 cut(s) 422, 769
Mox20I TGGCCA 1 cut(s) 5
MroXI GAANNNNTTC 1 cut(s) 680
MscI TGGCCA 1 cut(s) 5
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 9 cut(s) 464, 470, 497, 506, 517, 545, 579, 584, 768
MspR9I CCNGG 2 cut(s) 518, 580
Mva1269I GAATGC 1 cut(s) 44
MvnI CGCG 3 cut(s) 311, 416, 428
MwoI GCNNNNNNNGC 5 cut(s) 236, 245, 441, 632, 770
NarI GGCGCC 1 cut(s) 291
NciI CCSGG 2 cut(s) 518, 580
NdeII GATC 1 cut(s) 367
NlaIV GGNNCC 7 cut(s) 292, 393, 468, 504, 543, 754, 772
NmeAIII GCCGAG 4 cut(s) 294, 352, 411, 435
NmuCI GTSAC 1 cut(s) 22
PciSI GCTCTTC 1 cut(s) 671
PcsI WCGNNNNNNNCGW 3 cut(s) 345, 413, 432
PctI GAATGC 1 cut(s) 44
PdmI GAANNNNTTC 1 cut(s) 680
PfeI GAWTC 2 cut(s) 612, 839
Pfl23II CGTACG 1 cut(s) 561
PflFI GACNNNGTC 1 cut(s) 260
PflMI CCANNNNNTGG 1 cut(s) 604
PfoI TCCNGGA 1 cut(s) 516
PinAI ACCGGT 1 cut(s) 469
PkrI GCNGC 9 cut(s) 70, 238, 250, 310, 443, 446, 595, 745, 766
PluTI GGCGCC 1 cut(s) 294
Psp124BI GAGCTC 2 cut(s) 333, 525
PspFI CCCAGC 2 cut(s) 697, 702
PspLI CGTACG 1 cut(s) 561
PspN4I GGNNCC 7 cut(s) 292, 393, 468, 504, 543, 754, 772
PspOMI GGGCCC 1 cut(s) 752
PspPI GGNCC 7 cut(s) 391, 576, 581, 595, 745, 752, 753
PsyI GACNNNGTC 1 cut(s) 260
RsaI GTAC 2 cut(s) 468, 563
RsaNI GTAC 2 cut(s) 467, 562
SacI GAGCTC 2 cut(s) 333, 525
SapI GCTCTTC 1 cut(s) 671
SatI GCNGC 9 cut(s) 69, 237, 249, 309, 442, 445, 594, 744, 765
Sau3AI GATC 1 cut(s) 367
Sau96I GGNCC 7 cut(s) 391, 576, 581, 595, 745, 752, 753
ScrFI CCNGG 2 cut(s) 518, 580
SduI GDGCHC 5 cut(s) 333, 525, 544, 756, 775
SfaNI GCATC 1 cut(s) 622
SfoI GGCGCC 1 cut(s) 292
SinI GGWCC 1 cut(s) 391
SmlI CTYRAG 1 cut(s) 353
SmoI CTYRAG 1 cut(s) 353
Sse9I AATT 2 cut(s) 384, 718
SspDI GGCGCC 1 cut(s) 290
SspMI CTAG 1 cut(s) 648
SstI GAGCTC 2 cut(s) 333, 525
StyD4I CCNGG 2 cut(s) 516, 578
TaaI ACNGT 4 cut(s) 40, 133, 154, 304
TaiI ACGT 1 cut(s) 264
TaqI TCGA 3 cut(s) 336, 364, 816
TasI AATT 2 cut(s) 384, 718
TauI GCSGC 9 cut(s) 71, 239, 251, 311, 444, 447, 596, 746, 767
TfiI GAWTC 2 cut(s) 612, 839
TscAI CASTG 1 cut(s) 658
TseFI GTSAC 1 cut(s) 22
Tsp45I GTSAC 1 cut(s) 22
TspRI CASTG 1 cut(s) 658
Tth111I GACNNNGTC 1 cut(s) 260
Van91I CCANNNNNTGG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 391
XapI RAATTY 1 cut(s) 384
XmnI GAANNNNTTC 1 cut(s) 680
XspI CTAG 1 cut(s) 648
ZraI GACGTC 1 cut(s) 262
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.