Prupe.7G252900_v2.0.a1

Uroporphyrinogen-III synthase HemD

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp07
OG0016720
Physical Location & Seq
Forward (+)
21333355 .. 21334626
1272 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.7G252900.1

Sequence Viewer

Length: 864 bp
ATGAGCGTGCCCACAGCCGCACCCACAGTTGCCTTCACCACCCCTCCAAACTACGCCGCCAGGCTGGCCCACCTCCTCGCCCTCAAAGGATTCAACCCTATCTCCTCTCCCACGCTCATCGTCCAGCCCACCCCATCCACCATCTCCGCTCTCAAACCCTACCTCTCGCCACCCCCTTCCCTCGACCTCTTCTCCGCCATCGCCTTCCCTTCACGCACCGCAATCACCTCCCTTTCCGCGGCCGCCGCCGACATCAGTCACCCTCTCCTGTCGCCGCATGGCGATGCATTCATTATCGCCGCGCTGGGCAAGGACGCCGAGCTCATGGACGACAACTTCGTCCATAAGCTCTGTTCAAATACTAACCGGGTTAGAATTTTAGTCCCTCCGACTGCGACTCCGAGCGGCTTAGTTGAAGCGCTAGGAGACGGACGCAATCGGAGGGTGTTGTGTCCAGTCCCGGTCGTGGTGGGACTGGTTGAGCCTCCTGTGGTCCCGGATTTTCTCCGAGACTTGGAGGCCAAGCGTTGGGTTCCGGTTAGGGTTAATGCGTACGAGACGCGGTGGGCCGGGCCGGGGTGCGCTAAGCAAGTGGTGGAGAGGATTGAGGAGGGAGCGTTGGATGCGATGGTGTTCACGAGCACTGCGGAGGTGGAGGGCTTACTGAAGAGCTTCAAAGAGTTTGGGCTGGATTGGGAGATTGCCAAGAAGAGGTGTCCCAAGATGTTGGTTGCGGCCCACGGGCCCATCACGGCGGCTGGGGCCCACATGCTTGGGGTTAGAGTTGATTTGGTGAGCTCTCAATTTGATAGCTTTCAAGGCGTCGTTGATGCTTTACACACTGAGATTTCAAGGCTGAGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HEM4 RsfS

Protein Analysis

288

Amino Acids

30.63

Weight (kDa)

6.66

Isoelectric Point (pI)

44.34

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016720)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g01510
malus_domestica MD15G1157700.v1.1
prunus_persica Prupe.7G252900_v2.0.a1
pyrus_communis pycom15g14150
rosa_chinensis RchiOBHm_Chr2g0086431
rosa_laevigata RLG00000015758
rosa_multiflora Rmu_sc0005127.1_g000004
rosa_roxburghii Rroxscaffold_2G00154580
rosa_rugosa Rorug01G0467400
rosa_samantha Rh2AG018000 Rh2BG017700 Rh2CG018400 Rh2DG019300
rosa_wichuraiana Rw2G001510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 338
AccB7I CCANNNNNTGG 1 cut(s) 528
AccBSI CCGCTC 2 cut(s) 149, 405
AccII CGCG 3 cut(s) 239, 302, 562
AcoI YGGCCR 1 cut(s) 240
AcsI RAATTY 1 cut(s) 375
AcuI CTGAAG 1 cut(s) 686
AcyI GRCGYC 2 cut(s) 315, 822
AfaI GTAC 1 cut(s) 554
AfeI AGCGCT 1 cut(s) 420
AfiI CCNNNNNNNGG 6 cut(s) 238, 466, 514, 528, 576, 711
AgsI TTSAA 6 cut(s) 94, 357, 416, 676, 818, 852
AjnI CCWGG 1 cut(s) 59
AluBI AGCT 5 cut(s) 322, 349, 672, 798, 813
AluI AGCT 5 cut(s) 322, 349, 672, 798, 813
Alw21I GWGCWC 3 cut(s) 324, 644, 800
Alw26I GTCTC 3 cut(s) 420, 504, 551
Aor51HI AGCGCT 1 cut(s) 420
AoxI GGCC 8 cut(s) 66, 240, 519, 567, 572, 735, 743, 762
ApaI GGGCCC 2 cut(s) 747, 766
ApoI RAATTY 1 cut(s) 375
ArsI GACNNNNNNTTYG 2 cut(s) 320, 352
Asp700I GAANNNNTTC 1 cut(s) 671
AspLEI GCGC 3 cut(s) 304, 421, 584
AspS9I GGNCC 9 cut(s) 67, 493, 567, 572, 736, 743, 744, 762, 763
AsuC2I CCSGG 5 cut(s) 368, 461, 497, 571, 576
AsuHPI GGTGA 4 cut(s) 28, 217, 251, 805
AvaII GGWCC 1 cut(s) 493
BaeGI GKGCMC 3 cut(s) 12, 747, 766
BanII GRGCYC 4 cut(s) 324, 747, 766, 800
BauI CACGAG 1 cut(s) 637
Bbv12I GWGCWC 3 cut(s) 324, 644, 800
BccI CCATC 5 cut(s) 142, 149, 206, 622, 755
BceAI ACGGC 1 cut(s) 768
BciT130I CCWGG 1 cut(s) 61
BcnI CCSGG 5 cut(s) 368, 461, 497, 571, 576
BcoDI GTCTC 3 cut(s) 420, 504, 551
BfaI CTAG 1 cut(s) 422
BfoI RGCGCY 1 cut(s) 422
BglI GCCNNNNNGGC 1 cut(s) 65
BlpI GCTNAGC 1 cut(s) 585
Bme1390I CCNGG 6 cut(s) 61, 368, 461, 497, 571, 576
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 9 cut(s) 67, 493, 567, 572, 736, 743, 744, 762, 763
BmiI GGNNCC 5 cut(s) 495, 534, 745, 763, 764
BmrFI CCNGG 6 cut(s) 61, 368, 461, 497, 571, 576
BmsI GCATC 3 cut(s) 274, 613, 820
BoxI GACNNNNGTC 1 cut(s) 255
Bpu1102I GCTNAGC 1 cut(s) 585
BpuMI CCSGG 5 cut(s) 368, 461, 497, 571, 576
BsaHI GRCGYC 2 cut(s) 315, 822
BsaJI CCNNGG 3 cut(s) 237, 575, 739
BsaWI WCCGGW 1 cut(s) 535
Bsc4I CCNNNNNNNGG 6 cut(s) 238, 466, 514, 528, 576, 711
Bse1I ACTGG 2 cut(s) 455, 480
BseBI CCWGG 1 cut(s) 61
BseDI CCNNGG 3 cut(s) 237, 575, 739
BseGI GGATG 2 cut(s) 134, 628
BseLI CCNNNNNNNGG 6 cut(s) 238, 466, 514, 528, 576, 711
BseMII CTCAG 2 cut(s) 834, 848
BseNI ACTGG 2 cut(s) 455, 480
BseRI GAGGAG 3 cut(s) 65, 94, 623
BseSI GKGCMC 3 cut(s) 12, 747, 766
BseX3I CGGCCG 1 cut(s) 240
BseYI CCCAGC 2 cut(s) 304, 758
Bsh1236I CGCG 3 cut(s) 239, 302, 562
Bsh1285I CGRYCG 2 cut(s) 243, 465
BshFI GGCC 8 cut(s) 68, 242, 521, 569, 574, 737, 745, 764
BsiEI CGRYCG 2 cut(s) 243, 465
BsiHKAI GWGCWC 3 cut(s) 324, 644, 800
BsiSI CCGG 6 cut(s) 367, 461, 497, 536, 570, 575
BsiWI CGTACG 1 cut(s) 552
BslFI GGGAC 5 cut(s) 368, 443, 479, 486, 702
BslI CCNNNNNNNGG 6 cut(s) 238, 466, 514, 528, 576, 711
BsmAI GTCTC 3 cut(s) 420, 504, 551
BsmBI CGTCTC 2 cut(s) 420, 551
BsmFI GGGAC 5 cut(s) 368, 443, 479, 486, 702
BsmI GAATGC 1 cut(s) 287
BsnI GGCC 8 cut(s) 68, 242, 521, 569, 574, 737, 745, 764
Bsp120I GGGCCC 2 cut(s) 743, 762
Bsp1286I GDGCHC 6 cut(s) 12, 324, 644, 747, 766, 800
Bsp1720I GCTNAGC 1 cut(s) 585
BspANI GGCC 8 cut(s) 68, 242, 521, 569, 574, 737, 745, 764
BspCNI CTCAG 2 cut(s) 835, 849
BspFNI CGCG 3 cut(s) 239, 302, 562
BspLI GGNNCC 5 cut(s) 495, 534, 745, 763, 764
BspQI GCTCTTC 1 cut(s) 662
BsrBI CCGCTC 2 cut(s) 149, 405
BsrI ACTGG 2 cut(s) 455, 480
BssECI CCNNGG 3 cut(s) 237, 575, 739
BssNI GRCGYC 2 cut(s) 315, 822
BssSI CACGAG 1 cut(s) 637
Bst2BI CACGAG 1 cut(s) 637
Bst2UI CCWGG 1 cut(s) 61
Bst4CI ACNGT 1 cut(s) 28
Bst6I CTCTTC 3 cut(s) 194, 662, 704
BstACI GRCGYC 2 cut(s) 315, 822
BstC8I GCNNGC 2 cut(s) 8, 66
BstDEI CTNAG 4 cut(s) 409, 585, 843, 857
BstDSI CCRYGG 2 cut(s) 237, 739
BstF5I GGATG 2 cut(s) 134, 628
BstFNI CGCG 3 cut(s) 239, 302, 562
BstH2I RGCGCY 1 cut(s) 422
BstHHI GCGC 3 cut(s) 304, 421, 584
BstMAI GTCTC 3 cut(s) 420, 504, 551
BstMCI CGRYCG 2 cut(s) 243, 465
BstMWI GCNNNNNNNGC 5 cut(s) 65, 245, 623, 761, 819
BstNI CCWGG 1 cut(s) 61
BstNSI RCATGY 1 cut(s) 772
BstPAI GACNNNNGTC 1 cut(s) 255
BstSCI CCNGG 6 cut(s) 59, 366, 459, 495, 569, 574
BstSLI GKGCMC 3 cut(s) 12, 747, 766
BstUI CGCG 3 cut(s) 239, 302, 562
BstXI CCANNNNNNTGG 2 cut(s) 727, 773
BstZI CGGCCG 1 cut(s) 240
BsuRI GGCC 8 cut(s) 68, 242, 521, 569, 574, 737, 745, 764
BtgI CCRYGG 2 cut(s) 237, 739
BtgZI GCGATG 3 cut(s) 184, 297, 641
BtsCI GGATG 2 cut(s) 134, 628
BtsI GCAGTG 1 cut(s) 642
BtsIMutI CAGTG 2 cut(s) 642, 840
Cac8I GCNNGC 2 cut(s) 8, 66
CciNI GCGGCCGC 1 cut(s) 240
CfoI GCGC 3 cut(s) 304, 421, 584
Cfr13I GGNCC 9 cut(s) 67, 493, 567, 572, 736, 743, 744, 762, 763
Cfr42I CCGCGG 1 cut(s) 240
CseI GACGC 4 cut(s) 323, 441, 568, 811
Csp6I GTAC 1 cut(s) 553
CviAII CATG 3 cut(s) 278, 325, 769
CviQI GTAC 1 cut(s) 553
DdeI CTNAG 4 cut(s) 409, 585, 843, 857
DrdI GACNNNNNNGTC 1 cut(s) 338
DseDI GACNNNNNNGTC 1 cut(s) 338
EaeI YGGCCR 1 cut(s) 240
EagI CGGCCG 1 cut(s) 240
Eam1104I CTCTTC 3 cut(s) 194, 662, 704
EarI CTCTTC 3 cut(s) 194, 662, 704
EciI GGCGGA 1 cut(s) 184
Ecl136II GAGCTC 2 cut(s) 322, 798
EclXI CGGCCG 1 cut(s) 240
Eco24I GRGCYC 4 cut(s) 324, 747, 766, 800
Eco47I GGWCC 1 cut(s) 493
Eco47III AGCGCT 1 cut(s) 420
Eco52I CGGCCG 1 cut(s) 240
Eco53kI GAGCTC 2 cut(s) 322, 798
Eco57I CTGAAG 1 cut(s) 686
EcoICRI GAGCTC 2 cut(s) 322, 798
EcoO109I RGGNCCY 1 cut(s) 762
EcoRII CCWGG 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 289
EcoT38I GRGCYC 4 cut(s) 324, 747, 766, 800
Esp3I CGTCTC 2 cut(s) 420, 551
FaeI CATG 3 cut(s) 281, 328, 772
FaiI YATR 4 cut(s) 279, 326, 345, 770
FaqI GGGAC 5 cut(s) 368, 443, 479, 486, 702
FatI CATG 3 cut(s) 277, 324, 768
FokI GGATG 2 cut(s) 121, 635
FriOI GRGCYC 4 cut(s) 324, 747, 766, 800
FspBI CTAG 1 cut(s) 422
GlaI GCGC 3 cut(s) 303, 420, 583
GsaI CCCAGC 2 cut(s) 308, 762
HaeII RGCGCY 1 cut(s) 422
HaeIII GGCC 8 cut(s) 68, 242, 521, 569, 574, 737, 745, 764
HapII CCGG 6 cut(s) 367, 461, 497, 536, 570, 575
HgaI GACGC 4 cut(s) 323, 441, 568, 811
HhaI GCGC 3 cut(s) 304, 421, 584
Hin1I GRCGYC 2 cut(s) 315, 822
Hin1II CATG 3 cut(s) 281, 328, 772
Hin6I GCGC 3 cut(s) 302, 419, 582
HinP1I GCGC 3 cut(s) 302, 419, 582
HinfI GANTC 2 cut(s) 90, 397
HpaII CCGG 6 cut(s) 367, 461, 497, 536, 570, 575
HphI GGTGA 4 cut(s) 28, 217, 251, 805
Hpy166II GTNNAC 1 cut(s) 636
Hpy188I TCNGA 4 cut(s) 390, 402, 441, 509
Hpy188III TCNNGA 1 cut(s) 637
Hpy8I GTNNAC 1 cut(s) 636
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 4 cut(s) 43, 186, 214, 219
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4V TGCA 1 cut(s) 287
HpyF10VI GCNNNNNNNGC 5 cut(s) 65, 245, 623, 761, 819
HpyF3I CTNAG 4 cut(s) 409, 585, 843, 857
Hsp92I GRCGYC 2 cut(s) 315, 822
Hsp92II CATG 3 cut(s) 281, 328, 772
HspAI GCGC 3 cut(s) 302, 419, 582
KspI CCGCGG 1 cut(s) 240
LguI GCTCTTC 1 cut(s) 662
LmnI GCTCC 1 cut(s) 614
LweI GCATC 3 cut(s) 274, 613, 820
MaeI CTAG 1 cut(s) 422
MaeIII GTNAC 1 cut(s) 257
MbiI CCGCTC 2 cut(s) 149, 405
MboII GAAGA 3 cut(s) 181, 679, 721
MhlI GDGCHC 6 cut(s) 12, 324, 644, 747, 766, 800
MluCI AATT 2 cut(s) 375, 803
MlyI GAGTC 1 cut(s) 391
MmeI TCCRAC 2 cut(s) 413, 600
Mph1103I ATGCAT 1 cut(s) 289
MroXI GAANNNNTTC 1 cut(s) 671
MseI TTAA 1 cut(s) 546
MslI CAYNNNNRTG 1 cut(s) 282
MspA1I CMGCKG 1 cut(s) 239
MspI CCGG 6 cut(s) 367, 461, 497, 536, 570, 575
MspR9I CCNGG 6 cut(s) 61, 368, 461, 497, 571, 576
Mva1269I GAATGC 1 cut(s) 287
MvaI CCWGG 1 cut(s) 61
MvnI CGCG 3 cut(s) 239, 302, 562
MwoI GCNNNNNNNGC 5 cut(s) 65, 245, 623, 761, 819
NciI CCSGG 5 cut(s) 368, 461, 497, 571, 576
NlaIII CATG 3 cut(s) 281, 328, 772
NlaIV GGNNCC 5 cut(s) 495, 534, 745, 763, 764
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 1 cut(s) 257
NotI GCGGCCGC 1 cut(s) 240
NsiI ATGCAT 1 cut(s) 289
NspI RCATGY 1 cut(s) 772
PciSI GCTCTTC 1 cut(s) 662
PcsI WCGNNNNNNNCGW 1 cut(s) 336
PctI GAATGC 1 cut(s) 287
PdmI GAANNNNTTC 1 cut(s) 671
PfeI GAWTC 1 cut(s) 90
Pfl23II CGTACG 1 cut(s) 552
PflMI CCANNNNNTGG 1 cut(s) 528
PfoI TCCNGGA 1 cut(s) 495
PleI GAGTC 1 cut(s) 391
PpsI GAGTC 1 cut(s) 391
PshAI GACNNNNGTC 1 cut(s) 255
Psp124BI GAGCTC 2 cut(s) 324, 800
Psp6I CCWGG 1 cut(s) 59
PspFI CCCAGC 2 cut(s) 304, 758
PspGI CCWGG 1 cut(s) 59
PspLI CGTACG 1 cut(s) 552
PspN4I GGNNCC 5 cut(s) 495, 534, 745, 763, 764
PspOMI GGGCCC 2 cut(s) 743, 762
PspPI GGNCC 9 cut(s) 67, 493, 567, 572, 736, 743, 744, 762, 763
RsaI GTAC 1 cut(s) 554
RsaNI GTAC 1 cut(s) 553
RseI CAYNNNNRTG 1 cut(s) 282
SacI GAGCTC 2 cut(s) 324, 800
SacII CCGCGG 1 cut(s) 240
SapI GCTCTTC 1 cut(s) 662
SaqAI TTAA 1 cut(s) 546
Sau96I GGNCC 9 cut(s) 67, 493, 567, 572, 736, 743, 744, 762, 763
SchI GAGTC 1 cut(s) 391
ScrFI CCNGG 6 cut(s) 61, 368, 461, 497, 571, 576
SduI GDGCHC 6 cut(s) 12, 324, 644, 747, 766, 800
SfaNI GCATC 3 cut(s) 274, 613, 820
Sfr303I CCGCGG 1 cut(s) 240
SgrBI CCGCGG 1 cut(s) 240
SinI GGWCC 1 cut(s) 493
SmiMI CAYNNNNRTG 1 cut(s) 282
Sse9I AATT 2 cut(s) 375, 803
SspMI CTAG 1 cut(s) 422
SstI GAGCTC 2 cut(s) 324, 800
StyD4I CCNGG 6 cut(s) 59, 366, 459, 495, 569, 574
TaaI ACNGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 183
TasI AATT 2 cut(s) 375, 803
TfiI GAWTC 1 cut(s) 90
Tru1I TTAA 1 cut(s) 546
Tru9I TTAA 1 cut(s) 546
TscAI CASTG 2 cut(s) 649, 847
TseFI GTSAC 1 cut(s) 257
Tsp45I GTSAC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 280
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 2 cut(s) 649, 847
Van91I CCANNNNNTGG 1 cut(s) 528
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 1 cut(s) 375
XceI RCATGY 1 cut(s) 772
XmnI GAANNNNTTC 1 cut(s) 671
XspI CTAG 1 cut(s) 422
Zsp2I ATGCAT 1 cut(s) 289
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.