FvH4_1g02010

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012555
Physical Location & Seq
Forward (+)
1052702 .. 1056316
3615 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02010.t1

Sequence Viewer

Length: 2226 bp
ATGATGGGGAAGCAAAGTGGGAAGAAGAAGAAAAGTTCAGGTGATAGTCCAAAGCCAAACCAAAGTAGTGACAATAGTCCGAAAACCCAAGATAAGGATACAGAAGTTTTCATTGCAATGTCTCATGAATTGAAAGATGAGGGGAATAAGTTGTTTCAGAAAAGGGATCATGAAGGAGCTATGTTGAAGTATGAGAAAGCTCTCAAATTGCTTCCGAGAAATCACATTGATGTGTCCTATCTTCGGAGTAATATGGCGGCTTGTTATATGCAGATGGGGCTGAGTGAGTATCCCAGGGCTATTAATGAGTGTAATTTGGCTCTTGAAGTTACACCTAAGTATAGTAAGGCACTGCTGAAGAGGGCAAAGTGTTATGAGGCTTTGAATAGGTTGGATTTGGCTCTTAGAGATGTTGGTACCGTGTTGGACATGGAACCAAACAATATCATGGCATTGGAGGTTGCAGAAAGAGTGAAAGATGTGCTAGAGAAGAAGGGATTGAGGGTGAATGACACGGTGATTGAGTTGCCTCCTGATTATGTAGAACCAAGTCACAATGTGCTGCCAAGGAAAGAAACAAAATCAAAGAGTCGGAAGAAGAAAGGGAACAAAGATGAAGACAAGAAAATTCATGAGGACAATACTAGTGGGAATACTGAAGACAAGATTGAGGAGAAGAAGGCTGTAGAAATAACTGAAGAAATGAAGTCTGTGGATATAGCTGAGGAGATGAAGTCTGTGGATATAGGTGAGGAAGATAAGGATGTGGAAATATCTGAGGAAAAGAAGGCAGATGTGAAGGTAGTTGTGGAGGAAAAGATCACTACAATGGAAGAAGTGCCGAAAAGATGTGTGAAATTGGTGTTGGGAGAGGATATAAGATGGGCTCAGTTGCCAGTAAATTGTACCCTCCTCCAACTAAGAGAGGTGATCAGCGATCGGTTTCCAAGATCAAAAGCAGTTCTTATTAAATATAGAGACCAGGAAGGTGATTTGGTCACCATCACTACTAATGAAGAGCTGAGATGGGCTGAAGAATCAGCAGAATCGGAAGGGTCTGTCAGGCTTTACATAGTAGAAGTTAGTCCTGATCAGGATCCATTCTTTGAGAAACTGAGAATTGAAGGGGACAAGCTTAACTATGGAGCTGAGAATGGTACCATGGTAAAACAAAAGGACATGAAGGGGTCACCTTGCATTGAGGATTGGATTATTGATTTTGCTCAGCTATTCAAGAACTATGCTGGGCTTGAATCTGATGCATACTTGGATCTTCATGAACTTGGGATGAAGTTTTACTCTGAGGCTATGGAGGAGACAGTTACTAGTGAAGAAGCTCAAGACATTTTCGACATAGCAGGAGAGAAGTTCCAGGAAATGGGAGCTTTGGCATTGTTCAATTGGGGAAATGTTCACATGGCCAGGGCAAGGAAGAAGGTATATATCGCAGAAGATTCTACCAAAGAGTCTATCTTTTCAACCATTAAAGCTGCATATGAATGGGCACAGAAAGAATACATGGAAGCTGGAAAGAGATATAAAGAAGCGTTGCACATCAAACCCGATTTCTATGAAGGTTATTTGGCCCTTGCGCAGCAGCAGTTTGAACAAGCTAAACTTTCTTGGTATTATGCAATTAGCAGCAGTGTTGATTTGGAGACTTGGCCCTCTACAGAGGTTCTAAGACTTTATAACAATGCCGAAGACAACATGGAAAAGGGCATGCAGCTGTGGGAGGAAGCAGAAGAGCAACGGTTGAGTGAACTCTCCAGTCCAACTAGTGGTAAAATCCAGTTGCAGAAAATGGGTTTGGATGGGATTGTCAAAGACCTATCAGCAAATGAAGCTGCAGAACAAGCTACAAACATGAGGTCTCAGATAAACCTTCTGTGGGGTACCATGCTCTATGAGCGATCCATTGTGGAGTTCAAATTGGGGTTACCTGTATGGCATGAATGCTTGGACGTTGCAGTTGAGAAGTTTGGACTTGCTGGGGCATCTTCAACTGATATAGCTGTTATGGTCAAAAACCACTCTTCAAACGATAATGCATTGGAAGGTCTAGGGTTCAAGATTGATGAGATAATACAAGCATGGAATGAGATGTATGAAGCTAAGAAGTGGCAGAGTGGAATTCCATCATTTCGTTTAGAGCCATTACTTCGGCGGAGAGTTTCAAAACTTCATTCTGCTTTGGAGAATGCTTTATCAGACAGCACTGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

742

Amino Acids

84.45

Weight (kDa)

5.05

Isoelectric Point (pI)

48.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 285 - 359 3.1e-13 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1692
Acc16I TGCGCA 1 cut(s) 1593
Acc65I GGTACC 3 cut(s) 416, 1157, 1895
AccB1I GGYRCC 3 cut(s) 416, 1157, 1895
AccB7I CCANNNNNTGG 2 cut(s) 1378, 1781
AciI CCGC 2 cut(s) 257, 2167
AclWI GGATC 5 cut(s) 174, 1091, 1104, 1278, 1908
AcoI YGGCCR 1 cut(s) 1419
AcsI RAATTY 2 cut(s) 627, 2133
AcuI CTGAAG 4 cut(s) 377, 678, 717, 1053
AdeI CACNNNGTG 1 cut(s) 559
AfaI GTAC 4 cut(s) 418, 907, 1159, 1897
AfiI CCNNNNNNNGG 6 cut(s) 94, 243, 1378, 1428, 1781, 1891
AhlI ACTAGT 3 cut(s) 644, 1325, 1778
AjnI CCWGG 4 cut(s) 293, 981, 1371, 1421
AjuI GAANNNNNNNTTGG 4 cut(s) 848, 880, 1793, 1825
Alw26I GTCTC 5 cut(s) 126, 972, 1310, 1652, 1878
AlwI GGATC 5 cut(s) 174, 1091, 1104, 1278, 1908
AoxI GGCC 3 cut(s) 1419, 1584, 1664
ApeKI GCWGC 7 cut(s) 562, 1490, 1594, 1597, 1641, 1726, 1847
ApoI RAATTY 2 cut(s) 627, 2133
ArsI GACNNNNNNTTYG 2 cut(s) 1856, 1888
AseI ATTAAT 1 cut(s) 303
Asp718I GGTACC 3 cut(s) 416, 1157, 1895
AspLEI GCGC 1 cut(s) 1594
AspS9I GGNCC 2 cut(s) 1585, 1665
AsuHPI GGTGA 8 cut(s) 53, 517, 529, 761, 940, 991, 1001, 1182
BaeGI GKGCMC 1 cut(s) 1507
BaeI ACNNNNGTAYC 4 cut(s) 90, 123, 1149, 1182
BalI TGGCCA 1 cut(s) 1421
BamHI GGATCC 1 cut(s) 1096
BanI GGYRCC 3 cut(s) 416, 1157, 1895
BanII GRGCYC 1 cut(s) 889
BbsI GAAGAC 3 cut(s) 624, 666, 1710
BbvCI CCTCAGC 1 cut(s) 723
BbvI GCAGC 7 cut(s) 549, 1477, 1606, 1609, 1653, 1738, 1834
BccI CCATC 6 cut(s) 268, 876, 1010, 1020, 1808, 2146
BciT130I CCWGG 4 cut(s) 295, 983, 1373, 1423
BciVI GTATCC 2 cut(s) 91, 300
BclI TGATCA 2 cut(s) 930, 1090
BcoDI GTCTC 5 cut(s) 126, 972, 1310, 1652, 1878
BcuI ACTAGT 3 cut(s) 644, 1325, 1778
BfaI CTAG 5 cut(s) 485, 645, 1326, 1779, 2063
BfmI CTRYAG 3 cut(s) 684, 1671, 1848
BfuI GTATCC 2 cut(s) 91, 300
BisI GCNGC 8 cut(s) 258, 563, 1491, 1595, 1598, 1642, 1727, 1848
BlpI GCTNAGC 1 cut(s) 1224
BlsI GCNGC 8 cut(s) 259, 564, 1492, 1596, 1599, 1643, 1728, 1849
Bme1390I CCNGG 4 cut(s) 295, 983, 1373, 1423
BmgT120I GGNCC 2 cut(s) 1585, 1665
BmiI GGNNCC 5 cut(s) 418, 435, 1098, 1159, 1897
BmrFI CCNGG 4 cut(s) 295, 983, 1373, 1423
BmsI GCATC 2 cut(s) 1249, 2006
BoxI GACNNNNGTC 1 cut(s) 75
BpiI GAAGAC 3 cut(s) 624, 666, 1710
BpmI CTGGAG 1 cut(s) 1753
Bpu10I CCTNAGC 1 cut(s) 723
Bpu1102I GCTNAGC 1 cut(s) 1224
BpuEI CTTGAG 1 cut(s) 1323
BsaBI GATNNNNATC 1 cut(s) 1095
BsaI GGTCTC 2 cut(s) 972, 1878
BsaJI CCNNGG 5 cut(s) 293, 294, 566, 1161, 1422
BsaXI ACNNNNNCTCC 2 cut(s) 719, 749
Bsc4I CCNNNNNNNGG 6 cut(s) 94, 243, 1378, 1428, 1781, 1891
Bse1I ACTGG 3 cut(s) 896, 1770, 1792
Bse3DI GCAATG 2 cut(s) 111, 123
Bse8I GATNNNNATC 1 cut(s) 1095
BseBI CCWGG 4 cut(s) 295, 983, 1373, 1423
BseDI CCNNGG 5 cut(s) 293, 294, 566, 1161, 1422
BseGI GGATG 3 cut(s) 769, 1293, 1819
BseJI GATNNNNATC 1 cut(s) 1095
BseLI CCNNNNNNNGG 6 cut(s) 94, 243, 1378, 1428, 1781, 1891
BseMI GCAATG 2 cut(s) 111, 123
BseNI ACTGG 3 cut(s) 896, 1770, 1792
BseRI GAGGAG 4 cut(s) 686, 740, 902, 1328
BseSI GKGCMC 1 cut(s) 1507
BseXI GCAGC 7 cut(s) 549, 1477, 1606, 1609, 1653, 1738, 1834
BseYI CCCAGC 2 cut(s) 1244, 1991
Bsh1285I CGRYCG 1 cut(s) 940
BshFI GGCC 3 cut(s) 1421, 1586, 1666
BshNI GGYRCC 3 cut(s) 416, 1157, 1895
BsiEI CGRYCG 1 cut(s) 940
BslFI GGGAC 1 cut(s) 1142
BslI CCNNNNNNNGG 6 cut(s) 94, 243, 1378, 1428, 1781, 1891
BsmAI GTCTC 5 cut(s) 126, 972, 1310, 1652, 1878
BsmFI GGGAC 1 cut(s) 1142
BsmI GAATGC 2 cut(s) 1961, 2206
BsnI GGCC 3 cut(s) 1421, 1586, 1666
Bso31I GGTCTC 2 cut(s) 972, 1878
Bsp1286I GDGCHC 2 cut(s) 889, 1507
Bsp143I GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
Bsp1720I GCTNAGC 1 cut(s) 1224
Bsp19I CCATGG 1 cut(s) 1161
BspACI CCGC 2 cut(s) 257, 2167
BspANI GGCC 3 cut(s) 1421, 1586, 1666
BspHI TCATGA 4 cut(s) 124, 169, 631, 1276
BspLI GGNNCC 5 cut(s) 418, 435, 1098, 1159, 1897
BspMAI CTGCAG 1 cut(s) 1852
BspPI GGATC 5 cut(s) 174, 1091, 1104, 1278, 1908
BspQI GCTCTTC 2 cut(s) 1011, 1740
BspT107I GGYRCC 3 cut(s) 416, 1157, 1895
BspTNI GGTCTC 2 cut(s) 972, 1878
BsrDI GCAATG 2 cut(s) 111, 123
BsrI ACTGG 3 cut(s) 896, 1770, 1792
BssECI CCNNGG 5 cut(s) 293, 294, 566, 1161, 1422
BssMI GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
BssT1I CCWWGG 2 cut(s) 566, 1161
Bst2UI CCWGG 4 cut(s) 295, 983, 1373, 1423
Bst4CI ACNGT 4 cut(s) 421, 517, 1321, 1755
Bst6I CTCTTC 4 cut(s) 353, 1011, 1740, 2041
BstC8I GCNNGC 1 cut(s) 1724
BstDSI CCRYGG 1 cut(s) 1161
BstEII GGTNACC 3 cut(s) 997, 1188, 1938
BstF5I GGATG 3 cut(s) 769, 1293, 1819
BstHHI GCGC 1 cut(s) 1594
BstKTI GATC 9 cut(s) 169, 822, 933, 940, 953, 1093, 1099, 1273, 1916
BstMAI GTCTC 5 cut(s) 126, 972, 1310, 1652, 1878
BstMBI GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
BstMCI CGRYCG 1 cut(s) 940
BstMWI GCNNNNNNNGC 4 cut(s) 277, 1844, 1856, 1909
BstNI CCWGG 4 cut(s) 295, 983, 1373, 1423
BstNSI RCATGY 1 cut(s) 1726
BstPAI GACNNNNGTC 1 cut(s) 75
BstPI GGTNACC 3 cut(s) 997, 1188, 1938
BstSCI CCNGG 4 cut(s) 293, 981, 1371, 1421
BstSFI CTRYAG 3 cut(s) 684, 1671, 1848
BstSLI GKGCMC 1 cut(s) 1507
BstV1I GCAGC 7 cut(s) 549, 1477, 1606, 1609, 1653, 1738, 1834
BstV2I GAAGAC 3 cut(s) 624, 666, 1710
BstX2I RGATCY 2 cut(s) 1096, 1270
BstYI RGATCY 2 cut(s) 1096, 1270
BsuI GTATCC 2 cut(s) 91, 300
BsuRI GGCC 3 cut(s) 1421, 1586, 1666
BtgI CCRYGG 1 cut(s) 1161
BtsCI GGATG 3 cut(s) 769, 1293, 1819
BtsI GCAGTG 2 cut(s) 350, 1651
BtsIMutI CAGTG 3 cut(s) 350, 1651, 2217
Cac8I GCNNGC 1 cut(s) 1724
CciI TCATGA 4 cut(s) 124, 169, 631, 1276
CfoI GCGC 1 cut(s) 1594
Cfr13I GGNCC 2 cut(s) 1585, 1665
Csp6I GTAC 4 cut(s) 417, 906, 1158, 1896
CspCI CAANNNNNGTGG 2 cut(s) 628, 663
CviQI GTAC 4 cut(s) 417, 906, 1158, 1896
DpnI GATC 9 cut(s) 168, 821, 932, 939, 952, 1092, 1098, 1272, 1915
DpnII GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
DraIII CACNNNGTG 1 cut(s) 559
EaeI YGGCCR 1 cut(s) 1419
Eam1104I CTCTTC 4 cut(s) 353, 1011, 1740, 2041
EarI CTCTTC 4 cut(s) 353, 1011, 1740, 2041
EciI GGCGGA 1 cut(s) 2182
Eco130I CCWWGG 2 cut(s) 566, 1161
Eco24I GRGCYC 1 cut(s) 889
Eco31I GGTCTC 2 cut(s) 972, 1878
Eco57I CTGAAG 4 cut(s) 377, 678, 717, 1053
Eco91I GGTNACC 3 cut(s) 997, 1188, 1938
EcoO65I GGTNACC 3 cut(s) 997, 1188, 1938
EcoRI GAATTC 1 cut(s) 2133
EcoRII CCWGG 4 cut(s) 293, 981, 1371, 1421
EcoT14I CCWWGG 2 cut(s) 566, 1161
EcoT22I ATGCAT 2 cut(s) 1264, 2053
EcoT38I GRGCYC 1 cut(s) 889
ErhI CCWWGG 2 cut(s) 566, 1161
FalI AAGNNNNNCTT 4 cut(s) 948, 980, 1602, 1634
FaqI GGGAC 1 cut(s) 1142
FauNDI CATATG 1 cut(s) 1495
FbaI TGATCA 2 cut(s) 930, 1090
Fnu4HI GCNGC 8 cut(s) 258, 563, 1491, 1595, 1598, 1642, 1727, 1848
FokI GGATG 3 cut(s) 776, 1300, 1826
FriOI GRGCYC 1 cut(s) 889
Fsp4HI GCNGC 8 cut(s) 258, 563, 1491, 1595, 1598, 1642, 1727, 1848
FspBI CTAG 5 cut(s) 485, 645, 1326, 1779, 2063
FspI TGCGCA 1 cut(s) 1593
GlaI GCGC 1 cut(s) 1593
GluI GCNGC 8 cut(s) 258, 563, 1491, 1595, 1598, 1642, 1727, 1848
GsaI CCCAGC 2 cut(s) 1248, 1995
GsuI CTGGAG 1 cut(s) 1753
HaeIII GGCC 3 cut(s) 1421, 1586, 1666
HhaI GCGC 1 cut(s) 1594
Hin6I GCGC 1 cut(s) 1592
HinP1I GCGC 1 cut(s) 1592
HindIII AAGCTT 1 cut(s) 1133
HinfI GANTC 6 cut(s) 589, 1037, 1046, 1253, 1454, 1466
HphI GGTGA 8 cut(s) 53, 517, 529, 761, 940, 991, 1001, 1182
Hpy166II GTNNAC 2 cut(s) 1414, 1763
Hpy8I GTNNAC 2 cut(s) 1414, 1763
HpyCH4III ACNGT 4 cut(s) 421, 517, 1321, 1755
HpyCH4IV ACGT 1 cut(s) 1965
HpyF10VI GCNNNNNNNGC 4 cut(s) 277, 1844, 1856, 1909
HpySE526I ACGT 1 cut(s) 1965
HspAI GCGC 1 cut(s) 1592
KpnI GGTACC 3 cut(s) 420, 1161, 1899
Ksp22I TGATCA 2 cut(s) 930, 1090
Kzo9I GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
LguI GCTCTTC 2 cut(s) 1011, 1740
LmnI GCTCC 3 cut(s) 176, 1145, 1382
Lsp1109I GCAGC 7 cut(s) 549, 1477, 1606, 1609, 1653, 1738, 1834
LweI GCATC 2 cut(s) 1249, 2006
MaeI CTAG 5 cut(s) 485, 645, 1326, 1779, 2063
MaeII ACGT 1 cut(s) 1965
MaeIII GTNAC 7 cut(s) 68, 328, 551, 997, 1188, 1321, 1938
MalI GATC 9 cut(s) 168, 821, 932, 939, 952, 1092, 1098, 1272, 1915
MboI GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
MfeI CAATTG 1 cut(s) 1399
MflI RGATCY 2 cut(s) 1096, 1270
MhlI GDGCHC 2 cut(s) 889, 1507
MlsI TGGCCA 1 cut(s) 1421
MluNI TGGCCA 1 cut(s) 1421
MlyI GAGTC 2 cut(s) 598, 1475
MmeI TCCRAC 5 cut(s) 372, 405, 572, 940, 1799
Mox20I TGGCCA 1 cut(s) 1421
Mph1103I ATGCAT 2 cut(s) 1264, 2053
MscI TGGCCA 1 cut(s) 1421
MseI TTAA 4 cut(s) 303, 969, 1137, 1485
MslI CAYNNNNRTG 5 cut(s) 116, 228, 230, 827, 1498
Msp20I TGGCCA 1 cut(s) 1421
MspA1I CMGCKG 1 cut(s) 1729
MspR9I CCNGG 4 cut(s) 295, 983, 1373, 1423
MunI CAATTG 1 cut(s) 1399
Mva1269I GAATGC 2 cut(s) 1961, 2206
MvaI CCWGG 4 cut(s) 295, 983, 1373, 1423
MwoI GCNNNNNNNGC 4 cut(s) 277, 1844, 1856, 1909
NcoI CCATGG 1 cut(s) 1161
NdeI CATATG 1 cut(s) 1495
NdeII GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
NlaIV GGNNCC 5 cut(s) 418, 435, 1098, 1159, 1897
NmuCI GTSAC 4 cut(s) 68, 551, 997, 1188
NsbI TGCGCA 1 cut(s) 1593
NsiI ATGCAT 2 cut(s) 1264, 2053
NspI RCATGY 1 cut(s) 1726
PaeI GCATGC 1 cut(s) 1726
PagI TCATGA 4 cut(s) 124, 169, 631, 1276
PasI CCCWGGG 1 cut(s) 294
PciSI GCTCTTC 2 cut(s) 1011, 1740
PctI GAATGC 2 cut(s) 1961, 2206
PfeI GAWTC 4 cut(s) 1037, 1046, 1253, 1454
PflMI CCANNNNNTGG 2 cut(s) 1378, 1781
PfoI TCCNGGA 1 cut(s) 1371
PkrI GCNGC 8 cut(s) 259, 564, 1492, 1596, 1599, 1643, 1728, 1849
Ple19I CGATCG 1 cut(s) 940
PleI GAGTC 2 cut(s) 597, 1474
PpsI GAGTC 2 cut(s) 597, 1474
PshAI GACNNNNGTC 1 cut(s) 75
PshBI ATTAAT 1 cut(s) 303
PsiI TTATAA 1 cut(s) 1692
Psp6I CCWGG 4 cut(s) 293, 981, 1371, 1421
PspEI GGTNACC 3 cut(s) 997, 1188, 1938
PspFI CCCAGC 2 cut(s) 1244, 1991
PspGI CCWGG 4 cut(s) 293, 981, 1371, 1421
PspN4I GGNNCC 5 cut(s) 418, 435, 1098, 1159, 1897
PspPI GGNCC 2 cut(s) 1585, 1665
PstI CTGCAG 1 cut(s) 1852
PsuI RGATCY 2 cut(s) 1096, 1270
PvuI CGATCG 1 cut(s) 940
PvuII CAGCTG 1 cut(s) 1729
RsaI GTAC 4 cut(s) 418, 907, 1159, 1897
RsaNI GTAC 4 cut(s) 417, 906, 1158, 1896
RseI CAYNNNNRTG 5 cut(s) 116, 228, 230, 827, 1498
SapI GCTCTTC 2 cut(s) 1011, 1740
SaqAI TTAA 4 cut(s) 303, 969, 1137, 1485
SatI GCNGC 8 cut(s) 258, 563, 1491, 1595, 1598, 1642, 1727, 1848
Sau3AI GATC 9 cut(s) 166, 819, 930, 937, 950, 1090, 1096, 1270, 1913
Sau96I GGNCC 2 cut(s) 1585, 1665
SchI GAGTC 2 cut(s) 598, 1475
ScrFI CCNGG 4 cut(s) 295, 983, 1373, 1423
SduI GDGCHC 2 cut(s) 889, 1507
SfaNI GCATC 2 cut(s) 1249, 2006
SfcI CTRYAG 3 cut(s) 684, 1671, 1848
SmiMI CAYNNNNRTG 5 cut(s) 116, 228, 230, 827, 1498
SmlI CTYRAG 1 cut(s) 1338
SmoI CTYRAG 1 cut(s) 1338
SpeI ACTAGT 3 cut(s) 644, 1325, 1778
SphI GCATGC 1 cut(s) 1726
SsiI CCGC 2 cut(s) 257, 2167
SspMI CTAG 5 cut(s) 485, 645, 1326, 1779, 2063
StyD4I CCNGG 4 cut(s) 293, 981, 1371, 1421
StyI CCWWGG 2 cut(s) 566, 1161
TaaI ACNGT 4 cut(s) 421, 517, 1321, 1755
TaiI ACGT 1 cut(s) 1968
TaqI TCGA 1 cut(s) 1350
TauI GCSGC 1 cut(s) 260
TfiI GAWTC 4 cut(s) 1037, 1046, 1253, 1454
Tru1I TTAA 4 cut(s) 303, 969, 1137, 1485
Tru9I TTAA 4 cut(s) 303, 969, 1137, 1485
TscAI CASTG 3 cut(s) 357, 1651, 2224
TseFI GTSAC 4 cut(s) 68, 551, 997, 1188
TseI GCWGC 7 cut(s) 562, 1490, 1594, 1597, 1641, 1726, 1847
Tsp45I GTSAC 4 cut(s) 68, 551, 997, 1188
TspRI CASTG 3 cut(s) 357, 1651, 2224
Van91I CCANNNNNTGG 2 cut(s) 1378, 1781
VspI ATTAAT 1 cut(s) 303
XapI RAATTY 2 cut(s) 627, 2133
XceI RCATGY 1 cut(s) 1726
XspI CTAG 5 cut(s) 485, 645, 1326, 1779, 2063
Zsp2I ATGCAT 2 cut(s) 1264, 2053
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.