MD02G1018300.v1.1

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012555
Physical Location & Seq
Forward (+)
1350243 .. 1353963
3721 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1018300.v1.1.491

Sequence Viewer

Length: 2193 bp
ATGATGGGGAAGCAAAGAGGAAAAAAGAAGAAAAATGCAGGAGAAATTTCAGGTGATAGTTCGAATCAAAGCGAAGTCGGGGATACTAGCCCGGAAGCCAATGATAAGGACACAGAAGCTTTCATCTCGATGTCTCAAGAACTGAAGGACGAGGGGAACAAGTTGTTTCAGAAGAGGGATGTTGAAGGAGCTTTGTTGAAATATGAGAAAGCCCTCAAACTGCTTCCGAGAAACCATATCGATGTATCCTATCTTAGGAGTAACATGGCAGCGTGCTATATGCAGATGGGGTTGAATGAGTATCCGAGGGCTATCAATGAGTGTAATTTGGTGCTTGAAGTCACGCCTACGTACAGTAAGGCGCTGTTGAAGAGGGCAAAGTGTTACGAGGCTTTGAATAGGTTGGATTTGGCTCTGAGAGATGTTGGCACTGTCTTGAACCTGGAGCCAAAAAATGTCATGGCAATGGAGATCGCAGAACGGGTGAAAAACGTACTTGAGGAGAAGGGGTTGAGGGTGAATGACACAGTAATTGAGTTGCCTCTGGATTATGTTGAACCAAGTCATGCTTTGCAGCCAGGGAAAGTAATGAAGTTGAAGAGTCGGAAGAAGAAGAAGGGAAAAAAAGATGCTGGGATGAAGTCCGAGGAGAATTTTGAGGAGAAGGCTGAGGAGAAGATCGAGGAAAAGAAGGCTGTGGACGTAGCTGAGGAAGAGAAGTCTGTGGAAATAGCTGAAGACAAGAAGGATGTGGAAATAACCGAGGAAAAGAAGGTAGAGGTCAAGGTAGTTGTGGAGGAGACAATCAGCAGCATAAAGGAAGAAGGGCCTAAAAGAAGTGTGAAATTGGTATTTGGAGAGGATATAAGATGGGCTCAGTTGCCTGTATATTGCACCCTCCTGCAACTAAGAGAGGTTGTTCGTGACCGGTTTCCAAGCTCCAAAGCAGTTCTTATCAAGTATAGAGACCAAGAAGGTGATTTGGTCACAATAACTAGTAACGAAGAGCTGAGATGGGCTGAAGGATCAACAGAATCGGAAGGGTCTGTCAGGCTTTACGTAGTGGAAGTTAACCCCAAGCAGGATCCCTTCTTCGAGAAACTGGAAATCGAGGAAAACAGGCAACATAGCGTAGCTGAGAATGGCGTGGTAAAACACAAGGATACAAAGAGGCCACCTTGCATTGAGGATTGGATAATCAGTTTTTCTCAGCTGTTCAAGAACTATGCCGGGATTGAATCTGATGCATACTTGGATCTTCATGAATTTGGCATGAAGCTTTATTCGGAGGCAATGGAGGAGACAATCACGAGTGAAGAAGCACAGGACCTGTTTGACATAGCAGGAATGAAGTTCCAAGAAATGGGAGCTTTAGCTCTGTTCAACTGGGGAAATGTTCACATGGCCAGGGCGAGGAAGAAGGTGTATTTCACCGAAGATCCTTCGAAAGAATCTATCATTTCAAATATCAAAAGTGCATACGATTGGGCACAGAAAGAATACATACAAGCAGGAAGGAGATACGAAGAAGCGCTGCAGATCAAACCAGACTTTTATGAAGGCTATCTGGCCCTCGGGCAGCAGCAGTTTGAACAGGCAAAACTTTCTTGGTATTATGCTATTAGCAGCAATGTTGATCTGGAGACGTGGCCTTCCACAGAGGTTCTGCGACTTTATAACTACGCCGAAGACAATATGGAAAAAGGCATGCAGCTGTTTGAGGAATTGGAAGAGCAACGCCTGACTGAACCGTCCTGTCCAAGTAGTGTCAAGACTCAGGTGCAGAAAATGGGTTTGAACGGAATTTTCAAAGACGTATCATCAAATGAAGCTGCAGACCAGGCTACAAGCATGAGGTGTCAGATCAATCTCCTATGGGGCACTATGCTCTACGAGCGATCTATTGTTGAATTCAAGTTAGGACTACCTGTATGGAACGAATGTTTGGAAGTCGCAGTTGAAAAATTTGGGCTCGCCGGGGCTTCACCAACAGATATAGCAGTCATGCTCAAAAACCACTCTTCAAATGATAATGCATTAGAAGGTCTGGGGTTCAAAATTGACGAGATAGTTCAAGCTTGGAACGAGATGCAAGAAGCGAAAAAGTGGCAGACTGGAATTCCATCATTCCGGTTAGAACCATTACTGCGAAGACGAGTTTCCAGAATTTATTCTGCCTTGGAGCATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

731

Amino Acids

83.42

Weight (kDa)

5.14

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 280 - 356 3.3e-13 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1677
AasI GACNNNNNNGTC 1 cut(s) 1750
AccB7I CCANNNNNTGG 1 cut(s) 1363
AclWI GGATC 5 cut(s) 1033, 1079, 1092, 1263, 1433
AcoI YGGCCR 1 cut(s) 1404
AcsI RAATTY 8 cut(s) 45, 652, 1265, 1803, 1910, 1964, 2118, 2166
AcuI CTGAAG 3 cut(s) 164, 756, 1041
AfaI GTAC 2 cut(s) 353, 495
AfeI AGCGCT 1 cut(s) 1533
AfiI CCNNNNNNNGG 4 cut(s) 255, 1081, 1363, 1413
AgeI ACCGGT 1 cut(s) 927
AhlI ACTAGT 1 cut(s) 995
AjiI CACGTC 1 cut(s) 1647
AjnI CCWGG 4 cut(s) 441, 577, 1406, 1839
AjuI GAANNNNNNNTTGG 2 cut(s) 1928, 1960
Alw26I GTCTC 5 cut(s) 138, 794, 960, 1295, 1637
AlwI GGATC 5 cut(s) 1033, 1079, 1092, 1263, 1433
AlwNI CAGNNNCTG 1 cut(s) 1330
Ama87I CYCGRG 1 cut(s) 1574
Aor51HI AGCGCT 1 cut(s) 1533
AoxI GGCC 5 cut(s) 827, 1172, 1404, 1569, 1649
ApeKI GCWGC 9 cut(s) 269, 574, 810, 1534, 1579, 1582, 1626, 1711, 1832
ApoI RAATTY 8 cut(s) 45, 652, 1265, 1803, 1910, 1964, 2118, 2166
AsiGI ACCGGT 1 cut(s) 927
Asp700I GAANNNNTTC 1 cut(s) 2170
AspLEI GCGC 2 cut(s) 364, 1534
AspS9I GGNCC 3 cut(s) 827, 1327, 1570
AsuC2I CCSGG 3 cut(s) 92, 1231, 1978
AsuHPI GGTGA 6 cut(s) 65, 496, 529, 989, 1423, 1977
AsuII TTCGAA 2 cut(s) 62, 1445
AvaI CYCGRG 1 cut(s) 1574
AvaII GGWCC 1 cut(s) 1327
BaeGI GKGCMC 2 cut(s) 1492, 1883
BalI TGGCCA 1 cut(s) 1406
BamHI GGATCC 1 cut(s) 1084
BanII GRGCYC 2 cut(s) 877, 1974
BauI CACGAG 1 cut(s) 1309
BbsI GAAGAC 3 cut(s) 744, 1695, 2158
BbvCI CCTCAGC 2 cut(s) 669, 708
BbvI GCAGC 9 cut(s) 281, 586, 822, 1521, 1591, 1594, 1638, 1723, 1819
BccI CCATC 4 cut(s) 280, 864, 1008, 2131
BciT130I CCWGG 4 cut(s) 443, 579, 1408, 1841
BciVI GTATCC 4 cut(s) 76, 256, 312, 1156
BcnI CCSGG 3 cut(s) 92, 1231, 1978
BcoDI GTCTC 5 cut(s) 138, 794, 960, 1295, 1637
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 2 cut(s) 87, 996
BfmI CTRYAG 2 cut(s) 1535, 1833
BfoI RGCGCY 2 cut(s) 365, 1535
BfuI GTATCC 4 cut(s) 76, 256, 312, 1156
BisI GCNGC 9 cut(s) 270, 575, 811, 1535, 1580, 1583, 1627, 1712, 1833
BlsI GCNGC 9 cut(s) 271, 576, 812, 1536, 1581, 1584, 1628, 1713, 1834
Bme1390I CCNGG 7 cut(s) 92, 443, 579, 1231, 1408, 1841, 1978
Bme18I GGWCC 1 cut(s) 1327
BmeT110I CYCGRG 1 cut(s) 1574
BmgBI CACGTC 1 cut(s) 1647
BmgT120I GGNCC 3 cut(s) 827, 1327, 1570
BmiI GGNNCC 2 cut(s) 447, 1086
BmrFI CCNGG 7 cut(s) 92, 443, 579, 1231, 1408, 1841, 1978
BmrI ACTGGG 1 cut(s) 1396
BmsI GCATC 3 cut(s) 619, 1234, 2079
BmuI ACTGGG 1 cut(s) 1396
BpiI GAAGAC 3 cut(s) 744, 1695, 2158
BpmI CTGGAG 2 cut(s) 464, 1661
Bpu10I CCTNAGC 2 cut(s) 669, 708
Bpu14I TTCGAA 2 cut(s) 62, 1445
BpuEI CTTGAG 2 cut(s) 120, 518
BpuMI CCSGG 3 cut(s) 92, 1231, 1978
Bsa29I ATCGAT 1 cut(s) 240
BsaAI YACGTR 2 cut(s) 351, 1060
BsaI GGTCTC 1 cut(s) 960
BsaJI CCNNGG 8 cut(s) 305, 578, 645, 762, 1407, 1573, 1977, 2178
BsaWI WCCGGW 2 cut(s) 927, 2130
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 4 cut(s) 255, 1081, 1363, 1413
Bse118I RCCGGY 1 cut(s) 927
Bse1I ACTGG 3 cut(s) 1107, 1391, 2119
Bse3DI GCAATG 3 cut(s) 471, 1299, 1636
BseBI CCWGG 4 cut(s) 443, 579, 1408, 1841
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 8 cut(s) 305, 578, 645, 762, 1407, 1573, 1977, 2178
BseGI GGATG 3 cut(s) 184, 642, 754
BseLI CCNNNNNNNGG 4 cut(s) 255, 1081, 1363, 1413
BseMI GCAATG 3 cut(s) 471, 1299, 1636
BseMII CTCAG 8 cut(s) 407, 660, 699, 890, 1001, 1128, 1223, 1790
BseNI ACTGG 3 cut(s) 1107, 1391, 2119
BseRI GAGGAG 6 cut(s) 515, 662, 674, 686, 812, 1313
BseSI GKGCMC 2 cut(s) 1492, 1883
BseXI GCAGC 9 cut(s) 281, 586, 822, 1521, 1591, 1594, 1638, 1723, 1819
BseYI CCCAGC 1 cut(s) 632
BsgI GTGCAG 1 cut(s) 1802
BshFI GGCC 5 cut(s) 829, 1174, 1406, 1571, 1651
BshTI ACCGGT 1 cut(s) 927
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 1 cut(s) 1574
BsiSI CCGG 5 cut(s) 92, 928, 1230, 1977, 2131
BslI CCNNNNNNNGG 4 cut(s) 255, 1081, 1363, 1413
BsmAI GTCTC 5 cut(s) 138, 794, 960, 1295, 1637
BsmBI CGTCTC 1 cut(s) 1637
BsnI GGCC 5 cut(s) 829, 1174, 1406, 1571, 1651
Bso31I GGTCTC 1 cut(s) 960
BsoBI CYCGRG 1 cut(s) 1574
Bsp119I TTCGAA 2 cut(s) 62, 1445
Bsp1286I GDGCHC 4 cut(s) 877, 1492, 1883, 1974
BspANI GGCC 5 cut(s) 829, 1174, 1406, 1571, 1651
BspCNI CTCAG 8 cut(s) 408, 661, 700, 889, 1002, 1129, 1222, 1789
BspDI ATCGAT 1 cut(s) 240
BspHI TCATGA 1 cut(s) 1261
BspLI GGNNCC 2 cut(s) 447, 1086
BspMAI CTGCAG 2 cut(s) 1539, 1837
BspPI GGATC 5 cut(s) 1033, 1079, 1092, 1263, 1433
BspQI GCTCTTC 2 cut(s) 999, 1725
BspT104I TTCGAA 2 cut(s) 62, 1445
BspTNI GGTCTC 1 cut(s) 960
BsrDI GCAATG 3 cut(s) 471, 1299, 1636
BsrFI RCCGGY 1 cut(s) 927
BsrI ACTGG 3 cut(s) 1107, 1391, 2119
BssAI RCCGGY 1 cut(s) 927
BssECI CCNNGG 8 cut(s) 305, 578, 645, 762, 1407, 1573, 1977, 2178
BssSI CACGAG 1 cut(s) 1309
BssT1I CCWWGG 1 cut(s) 2178
Bst2BI CACGAG 1 cut(s) 1309
Bst2UI CCWGG 4 cut(s) 443, 579, 1408, 1841
Bst4CI ACNGT 4 cut(s) 356, 433, 529, 1752
Bst6I CTCTTC 7 cut(s) 167, 365, 593, 708, 999, 1725, 2026
BstBAI YACGTR 2 cut(s) 351, 1060
BstBI TTCGAA 2 cut(s) 62, 1445
BstC8I GCNNGC 4 cut(s) 274, 1709, 1974, 2187
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 3 cut(s) 184, 642, 754
BstH2I RGCGCY 2 cut(s) 365, 1535
BstHHI GCGC 2 cut(s) 364, 1534
BstMAI GTCTC 5 cut(s) 138, 794, 960, 1295, 1637
BstMWI GCNNNNNNNGC 2 cut(s) 1841, 1894
BstNI CCWGG 4 cut(s) 443, 579, 1408, 1841
BstNSI RCATGY 2 cut(s) 1711, 2189
BstSCI CCNGG 7 cut(s) 90, 441, 577, 1229, 1406, 1839, 1976
BstSFI CTRYAG 2 cut(s) 1535, 1833
BstSLI GKGCMC 2 cut(s) 1492, 1883
BstSNI TACGTA 2 cut(s) 351, 1060
BstV1I GCAGC 9 cut(s) 281, 586, 822, 1521, 1591, 1594, 1638, 1723, 1819
BstV2I GAAGAC 3 cut(s) 744, 1695, 2158
BstX2I RGATCY 3 cut(s) 1084, 1255, 1438
BstYI RGATCY 3 cut(s) 1084, 1255, 1438
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 4 cut(s) 76, 256, 312, 1156
BsuRI GGCC 5 cut(s) 829, 1174, 1406, 1571, 1651
BsuTUI ATCGAT 1 cut(s) 240
BtrI CACGTC 1 cut(s) 1647
BtsCI GGATG 3 cut(s) 184, 642, 754
BtsIMutI CAGTG 1 cut(s) 429
Cac8I GCNNGC 4 cut(s) 274, 1709, 1974, 2187
CaiI CAGNNNCTG 1 cut(s) 1330
CciI TCATGA 1 cut(s) 1261
CfoI GCGC 2 cut(s) 364, 1534
Cfr10I RCCGGY 1 cut(s) 927
Cfr13I GGNCC 3 cut(s) 827, 1327, 1570
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 2 cut(s) 352, 494
CspAI ACCGGT 1 cut(s) 927
CviQI GTAC 2 cut(s) 352, 494
DrdI GACNNNNNNGTC 1 cut(s) 1750
DseDI GACNNNNNNGTC 1 cut(s) 1750
EaeI YGGCCR 1 cut(s) 1404
Eam1104I CTCTTC 7 cut(s) 167, 365, 593, 708, 999, 1725, 2026
EarI CTCTTC 7 cut(s) 167, 365, 593, 708, 999, 1725, 2026
Eco105I TACGTA 2 cut(s) 351, 1060
Eco130I CCWWGG 1 cut(s) 2178
Eco24I GRGCYC 2 cut(s) 877, 1974
Eco31I GGTCTC 1 cut(s) 960
Eco47I GGWCC 1 cut(s) 1327
Eco47III AGCGCT 1 cut(s) 1533
Eco57I CTGAAG 3 cut(s) 164, 756, 1041
Eco88I CYCGRG 1 cut(s) 1574
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 2 cut(s) 827, 1327
EcoRI GAATTC 2 cut(s) 1910, 2118
EcoRII CCWGG 4 cut(s) 441, 577, 1406, 1839
EcoT14I CCWWGG 1 cut(s) 2178
EcoT22I ATGCAT 2 cut(s) 1249, 2038
EcoT38I GRGCYC 2 cut(s) 877, 1974
ErhI CCWWGG 1 cut(s) 2178
Esp3I CGTCTC 1 cut(s) 1637
FalI AAGNNNNNCTT 4 cut(s) 553, 585, 936, 968
Fnu4HI GCNGC 9 cut(s) 270, 575, 811, 1535, 1580, 1583, 1627, 1712, 1833
FokI GGATG 3 cut(s) 191, 649, 761
FriOI GRGCYC 2 cut(s) 877, 1974
Fsp4HI GCNGC 9 cut(s) 270, 575, 811, 1535, 1580, 1583, 1627, 1712, 1833
FspBI CTAG 2 cut(s) 87, 996
GlaI GCGC 2 cut(s) 363, 1533
GluI GCNGC 9 cut(s) 270, 575, 811, 1535, 1580, 1583, 1627, 1712, 1833
GsaI CCCAGC 1 cut(s) 636
GsuI CTGGAG 2 cut(s) 464, 1661
HaeII RGCGCY 2 cut(s) 365, 1535
HaeIII GGCC 5 cut(s) 829, 1174, 1406, 1571, 1651
HapII CCGG 5 cut(s) 92, 928, 1230, 1977, 2131
HhaI GCGC 2 cut(s) 364, 1534
Hin6I GCGC 2 cut(s) 362, 1532
HinP1I GCGC 2 cut(s) 362, 1532
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HindIII AAGCTT 3 cut(s) 117, 1277, 2076
HinfI GANTC 6 cut(s) 64, 601, 1034, 1238, 1451, 1774
HpaI GTTAAC 1 cut(s) 1072
HpaII CCGG 5 cut(s) 92, 928, 1230, 1977, 2131
HphI GGTGA 6 cut(s) 65, 496, 529, 989, 1423, 1977
Hpy166II GTNNAC 3 cut(s) 700, 1072, 1399
Hpy8I GTNNAC 3 cut(s) 700, 1072, 1399
HpyCH4III ACNGT 4 cut(s) 356, 433, 529, 1752
HpyCH4IV ACGT 6 cut(s) 350, 492, 702, 1059, 1646, 1815
HpyF10VI GCNNNNNNNGC 2 cut(s) 1841, 1894
HpySE526I ACGT 6 cut(s) 350, 492, 702, 1059, 1646, 1815
HspAI GCGC 2 cut(s) 362, 1532
KspAI GTTAAC 1 cut(s) 1072
LguI GCTCTTC 2 cut(s) 999, 1725
LmnI GCTCC 5 cut(s) 188, 445, 944, 1367, 2182
Lsp1109I GCAGC 9 cut(s) 281, 586, 822, 1521, 1591, 1594, 1638, 1723, 1819
LweI GCATC 3 cut(s) 619, 1234, 2079
MaeI CTAG 2 cut(s) 87, 996
MaeII ACGT 6 cut(s) 350, 492, 702, 1059, 1646, 1815
MaeIII GTNAC 6 cut(s) 260, 340, 383, 923, 985, 998
MflI RGATCY 3 cut(s) 1084, 1255, 1438
MhlI GDGCHC 4 cut(s) 877, 1492, 1883, 1974
MlsI TGGCCA 1 cut(s) 1406
MluNI TGGCCA 1 cut(s) 1406
MlyI GAGTC 2 cut(s) 610, 1768
MmeI TCCRAC 2 cut(s) 384, 584
Mox20I TGGCCA 1 cut(s) 1406
Mph1103I ATGCAT 2 cut(s) 1249, 2038
MroXI GAANNNNTTC 1 cut(s) 2170
MscI TGGCCA 1 cut(s) 1406
MseI TTAA 1 cut(s) 1071
MslI CAYNNNNRTG 3 cut(s) 128, 240, 464
Msp20I TGGCCA 1 cut(s) 1406
MspA1I CMGCKG 2 cut(s) 1213, 1714
MspI CCGG 5 cut(s) 92, 928, 1230, 1977, 2131
MspR9I CCNGG 7 cut(s) 92, 443, 579, 1231, 1408, 1841, 1978
MvaI CCWGG 4 cut(s) 443, 579, 1408, 1841
MwoI GCNNNNNNNGC 2 cut(s) 1841, 1894
NciI CCSGG 3 cut(s) 92, 1231, 1978
NlaIV GGNNCC 2 cut(s) 447, 1086
NmuCI GTSAC 3 cut(s) 340, 923, 985
NsiI ATGCAT 2 cut(s) 1249, 2038
NspI RCATGY 2 cut(s) 1711, 2189
NspV TTCGAA 2 cut(s) 62, 1445
PaeI GCATGC 2 cut(s) 1711, 2189
PagI TCATGA 1 cut(s) 1261
PciSI GCTCTTC 2 cut(s) 999, 1725
PdmI GAANNNNTTC 1 cut(s) 2170
PfeI GAWTC 4 cut(s) 64, 1034, 1238, 1451
PflMI CCANNNNNTGG 1 cut(s) 1363
PinAI ACCGGT 1 cut(s) 927
PkrI GCNGC 9 cut(s) 271, 576, 812, 1536, 1581, 1584, 1628, 1713, 1834
PleI GAGTC 2 cut(s) 609, 1768
PpsI GAGTC 2 cut(s) 609, 1768
Ppu21I YACGTR 2 cut(s) 351, 1060
PpuMI RGGWCCY 1 cut(s) 1327
PsiI TTATAA 1 cut(s) 1677
Psp5II RGGWCCY 1 cut(s) 1327
Psp6I CCWGG 4 cut(s) 441, 577, 1406, 1839
PspFI CCCAGC 1 cut(s) 632
PspGI CCWGG 4 cut(s) 441, 577, 1406, 1839
PspN4I GGNNCC 2 cut(s) 447, 1086
PspPI GGNCC 3 cut(s) 827, 1327, 1570
PspPPI RGGWCCY 1 cut(s) 1327
PstI CTGCAG 2 cut(s) 1539, 1837
PstNI CAGNNNCTG 1 cut(s) 1330
PsuI RGATCY 3 cut(s) 1084, 1255, 1438
PvuII CAGCTG 2 cut(s) 1213, 1714
RsaI GTAC 2 cut(s) 353, 495
RsaNI GTAC 2 cut(s) 352, 494
RseI CAYNNNNRTG 3 cut(s) 128, 240, 464
SapI GCTCTTC 2 cut(s) 999, 1725
SaqAI TTAA 1 cut(s) 1071
SatI GCNGC 9 cut(s) 270, 575, 811, 1535, 1580, 1583, 1627, 1712, 1833
Sau96I GGNCC 3 cut(s) 827, 1327, 1570
SchI GAGTC 2 cut(s) 610, 1768
ScrFI CCNGG 7 cut(s) 92, 443, 579, 1231, 1408, 1841, 1978
SduI GDGCHC 4 cut(s) 877, 1492, 1883, 1974
SfaNI GCATC 3 cut(s) 619, 1234, 2079
SfcI CTRYAG 2 cut(s) 1535, 1833
SfuI TTCGAA 2 cut(s) 62, 1445
SinI GGWCC 1 cut(s) 1327
SmiMI CAYNNNNRTG 3 cut(s) 128, 240, 464
SmlI CTYRAG 2 cut(s) 135, 497
SmoI CTYRAG 2 cut(s) 135, 497
SnaBI TACGTA 2 cut(s) 351, 1060
SpeI ACTAGT 1 cut(s) 995
SphI GCATGC 2 cut(s) 1711, 2189
SspMI CTAG 2 cut(s) 87, 996
StyD4I CCNGG 7 cut(s) 90, 441, 577, 1229, 1406, 1839, 1976
StyI CCWWGG 1 cut(s) 2178
TaaI ACNGT 4 cut(s) 356, 433, 529, 1752
TaiI ACGT 6 cut(s) 353, 495, 705, 1062, 1649, 1818
TaqI TCGA 7 cut(s) 62, 128, 240, 681, 1095, 1110, 1445
TfiI GAWTC 4 cut(s) 64, 1034, 1238, 1451
Tru1I TTAA 1 cut(s) 1071
Tru9I TTAA 1 cut(s) 1071
TscAI CASTG 1 cut(s) 436
TseFI GTSAC 3 cut(s) 340, 923, 985
TseI GCWGC 9 cut(s) 269, 574, 810, 1534, 1579, 1582, 1626, 1711, 1832
Tsp45I GTSAC 3 cut(s) 340, 923, 985
TspDTI ATGAA 9 cut(s) 112, 605, 653, 1250, 1278, 1289, 1364, 1572, 1842
TspGWI ACGGA 1 cut(s) 1815
TspRI CASTG 1 cut(s) 436
Van91I CCANNNNNTGG 1 cut(s) 1363
VpaK11BI GGWCC 1 cut(s) 1327
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 8 cut(s) 45, 652, 1265, 1803, 1910, 1964, 2118, 2166
XceI RCATGY 2 cut(s) 1711, 2189
XmnI GAANNNNTTC 1 cut(s) 2170
XspI CTAG 2 cut(s) 87, 996
Zsp2I ATGCAT 2 cut(s) 1249, 2038
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.