pycom02g01640

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0012555
Physical Location & Seq
Forward (+)
976351 .. 977481
1131 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g01640.1

Sequence Viewer

Length: 1131 bp
ATGATGGGGAAGCAAAGAGGAAAAAAGAAGAAAAATGCAGGAGAAAATTCAGGTGGTAGTTCCAATCAAAGCGAAGTCGGGGATACTAGCCCAGAAGCCAGTGATAAGGACACAGAAGTTTTCATTTCAATGTCTCAAGAACTGAAGGACGAGGGGAACAAGTTGTTTCAGAAGAGGGATGTTGAAGGAGCTTTGTTGAAATATGAGAAAGCCCTCAAACTGCTTCCGAGAAACCATATCGATGTATCCTATCTTAGGAGTAACATGGCAGCGTGCTATATGCAGATGGGGTTGAGTGAATACCCGAGGGCTATCAATGAGTGTAATTTGGTGCTTGAAGTCACGCCTAAGTACTGTAAGGCGCTGTTGAAGAGGGCAAAGTGTTACGAGGCTTTGAATAGGTTGGATTTGGCTCTGAGAGATGTTGGCACTGTCTTGAACCTGGAGCCAAAAAATGTCATGGCAATGGAGATCGCAGAACGAGTGAAAAACGTACTTGAGGAGAAGGGGTTGAGGGTGAATGACACAGTAATTGAGTTGCCTCTGGATTATGTTGAACCAAGTCATGCTTTGCAGCCGGGGAAAGTAATGAAGTTGAAGAGTCGGAAGAAGAAGAAGGGAAAAAAAGATGATGAGATGAAGTCCGAGGAGAATTTTGAGGAGAAGGCTGAGGACAAGATTGAGGAAGAGAAGGCTGTGGACGTAGCTGAGGAAGAGAAGTCTGTGGAAATAGCTGAAGACAAGAAGGATGTGGAAATAATCGAGGAAAAGAAGGTAGAGGTCAAGGTAGTTGTGGAGGAGACAATCAGCAGCATAAAGGAAGAAGGGCCTAAAAGAAGTGTGAAATTGGTATTTGGAGAGGATATAAGATGGGCTCAGTTGCCTGTATATTGCACCCTCCTGCAACTAAGAGAGGTTGTTCGTGATCGGTTTCCAAGCTCCAAAGCAGTTCTTATCAAGTACAGAGACCAAGAAGGTGATTTGGTCACAATAACTAGTAACGAAGAGCTGAGATGGGCTGAAGGATCAACAGAATCGGAAGGGTCTGTCAGGCTTTACGTAGTGGAAGTTAACCCCGAGCAGAGATCCCTTCTTCGAGAAACTGGAAATCGAGGAAAACAATCAACATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

377

Amino Acids

42.7

Weight (kDa)

5.54

Isoelectric Point (pI)

53.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PB1 PF00564 280 - 356 1.2e-13 PB1 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 1033, 1080
AcsI RAATTY 2 cut(s) 46, 652
AcuI CTGAAG 3 cut(s) 164, 756, 1041
AfaI GTAC 3 cut(s) 353, 495, 962
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 9 cut(s) 129, 185, 199, 338, 370, 397, 439, 557, 598
AhlI ACTAGT 1 cut(s) 995
AjnI CCWGG 1 cut(s) 441
AluBI AGCT 5 cut(s) 191, 707, 734, 939, 1009
AluI AGCT 5 cut(s) 191, 707, 734, 939, 1009
Alw26I GTCTC 3 cut(s) 138, 794, 960
AlwI GGATC 2 cut(s) 1033, 1080
Ama87I CYCGRG 2 cut(s) 304, 1076
AoxI GGCC 1 cut(s) 827
ApeKI GCWGC 3 cut(s) 269, 574, 810
ApoI RAATTY 2 cut(s) 46, 652
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 1 cut(s) 827
AsuC2I CCSGG 1 cut(s) 579
AsuHPI GGTGA 2 cut(s) 529, 989
AvaI CYCGRG 2 cut(s) 304, 1076
BanII GRGCYC 1 cut(s) 877
BbsI GAAGAC 1 cut(s) 744
BbvCI CCTCAGC 2 cut(s) 669, 708
BbvI GCAGC 3 cut(s) 281, 586, 822
BccI CCATC 3 cut(s) 280, 864, 1008
BciT130I CCWGG 1 cut(s) 443
BciVI GTATCC 2 cut(s) 76, 256
BcnI CCSGG 1 cut(s) 579
BcoDI GTCTC 3 cut(s) 138, 794, 960
BcuI ACTAGT 1 cut(s) 995
BfaI CTAG 2 cut(s) 87, 996
BfoI RGCGCY 1 cut(s) 365
BfuI GTATCC 2 cut(s) 76, 256
BisI GCNGC 3 cut(s) 270, 575, 811
BlsI GCNGC 3 cut(s) 271, 576, 812
BmcAI AGTACT 1 cut(s) 353
Bme1390I CCNGG 2 cut(s) 443, 579
BmeT110I CYCGRG 2 cut(s) 304, 1076
BmgT120I GGNCC 1 cut(s) 827
BmiI GGNNCC 1 cut(s) 447
BmrFI CCNGG 2 cut(s) 443, 579
BpiI GAAGAC 1 cut(s) 744
BpmI CTGGAG 1 cut(s) 464
Bpu10I CCTNAGC 2 cut(s) 669, 708
BpuEI CTTGAG 2 cut(s) 120, 518
BpuMI CCSGG 1 cut(s) 579
Bsa29I ATCGAT 1 cut(s) 240
BsaAI YACGTR 1 cut(s) 1060
BsaI GGTCTC 1 cut(s) 960
BsaJI CCNNGG 3 cut(s) 305, 578, 645
BsaXI ACNNNNNCTCC 2 cut(s) 494, 524
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse1I ACTGG 2 cut(s) 99, 1108
Bse3DI GCAATG 1 cut(s) 471
BseBI CCWGG 1 cut(s) 443
BseCI ATCGAT 1 cut(s) 240
BseDI CCNNGG 3 cut(s) 305, 578, 645
BseGI GGATG 2 cut(s) 184, 754
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 471
BseMII CTCAG 5 cut(s) 407, 660, 699, 890, 1001
BseNI ACTGG 2 cut(s) 99, 1108
BseRI GAGGAG 4 cut(s) 515, 662, 674, 812
BseXI GCAGC 3 cut(s) 281, 586, 822
BshFI GGCC 1 cut(s) 829
BshVI ATCGAT 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 304, 1076
BsiSI CCGG 1 cut(s) 578
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 3 cut(s) 138, 794, 960
BsnI GGCC 1 cut(s) 829
Bso31I GGTCTC 1 cut(s) 960
BsoBI CYCGRG 2 cut(s) 304, 1076
Bsp1286I GDGCHC 1 cut(s) 877
Bsp143I GATC 4 cut(s) 471, 925, 1025, 1085
BspANI GGCC 1 cut(s) 829
BspCNI CTCAG 5 cut(s) 408, 661, 700, 889, 1002
BspDI ATCGAT 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 447
BspPI GGATC 2 cut(s) 1033, 1080
BspQI GCTCTTC 1 cut(s) 999
BspTNI GGTCTC 1 cut(s) 960
BsrDI GCAATG 1 cut(s) 471
BsrI ACTGG 2 cut(s) 99, 1108
BssECI CCNNGG 3 cut(s) 305, 578, 645
BssMI GATC 4 cut(s) 471, 925, 1025, 1085
Bst2UI CCWGG 1 cut(s) 443
Bst4CI ACNGT 3 cut(s) 356, 433, 529
Bst6I CTCTTC 6 cut(s) 167, 365, 593, 681, 708, 999
BstBAI YACGTR 1 cut(s) 1060
BstC8I GCNNGC 1 cut(s) 274
BstDEI CTNAG 8 cut(s) 254, 348, 416, 669, 708, 876, 908, 1010
BstENI CCTNNNNNAGG 1 cut(s) 253
BstF5I GGATG 2 cut(s) 184, 754
BstH2I RGCGCY 1 cut(s) 365
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 4 cut(s) 474, 928, 1028, 1088
BstMAI GTCTC 3 cut(s) 138, 794, 960
BstMBI GATC 4 cut(s) 471, 925, 1025, 1085
BstNI CCWGG 1 cut(s) 443
BstSCI CCNGG 2 cut(s) 441, 577
BstSNI TACGTA 1 cut(s) 1060
BstV1I GCAGC 3 cut(s) 281, 586, 822
BstV2I GAAGAC 1 cut(s) 744
BstX2I RGATCY 1 cut(s) 1085
BstYI RGATCY 1 cut(s) 1085
Bsu15I ATCGAT 1 cut(s) 240
BsuI GTATCC 2 cut(s) 76, 256
BsuRI GGCC 1 cut(s) 829
BsuTUI ATCGAT 1 cut(s) 240
BtsCI GGATG 2 cut(s) 184, 754
BtsIMutI CAGTG 2 cut(s) 106, 429
Cac8I GCNNGC 1 cut(s) 274
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 1 cut(s) 827
ClaI ATCGAT 1 cut(s) 240
Csp6I GTAC 3 cut(s) 352, 494, 961
CviAII CATG 3 cut(s) 265, 460, 566
CviQI GTAC 3 cut(s) 352, 494, 961
DdeI CTNAG 8 cut(s) 254, 348, 416, 669, 708, 876, 908, 1010
DpnI GATC 4 cut(s) 473, 927, 1027, 1087
DpnII GATC 4 cut(s) 471, 925, 1025, 1085
Eam1104I CTCTTC 6 cut(s) 167, 365, 593, 681, 708, 999
EarI CTCTTC 6 cut(s) 167, 365, 593, 681, 708, 999
Eco105I TACGTA 1 cut(s) 1060
Eco24I GRGCYC 1 cut(s) 877
Eco31I GGTCTC 1 cut(s) 960
Eco57I CTGAAG 3 cut(s) 164, 756, 1041
Eco88I CYCGRG 2 cut(s) 304, 1076
EcoNI CCTNNNNNAGG 1 cut(s) 253
EcoO109I RGGNCCY 1 cut(s) 827
EcoRII CCWGG 1 cut(s) 441
EcoT38I GRGCYC 1 cut(s) 877
FaeI CATG 3 cut(s) 268, 463, 569
FalI AAGNNNNNCTT 4 cut(s) 553, 585, 936, 968
FatI CATG 3 cut(s) 264, 459, 565
Fnu4HI GCNGC 3 cut(s) 270, 575, 811
FokI GGATG 2 cut(s) 191, 761
FriOI GRGCYC 1 cut(s) 877
Fsp4HI GCNGC 3 cut(s) 270, 575, 811
FspBI CTAG 2 cut(s) 87, 996
GlaI GCGC 1 cut(s) 363
GluI GCNGC 3 cut(s) 270, 575, 811
GsuI CTGGAG 1 cut(s) 464
HaeII RGCGCY 1 cut(s) 365
HaeIII GGCC 1 cut(s) 829
HapII CCGG 1 cut(s) 578
HhaI GCGC 1 cut(s) 364
Hin1II CATG 3 cut(s) 268, 463, 569
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HincII GTYRAC 1 cut(s) 1072
HindII GTYRAC 1 cut(s) 1072
HinfI GANTC 2 cut(s) 601, 1034
HpaI GTTAAC 1 cut(s) 1072
HpaII CCGG 1 cut(s) 578
HphI GGTGA 2 cut(s) 529, 989
Hpy166II GTNNAC 2 cut(s) 700, 1072
Hpy188I TCNGA 6 cut(s) 171, 228, 417, 606, 646, 1039
Hpy188III TCNNGA 5 cut(s) 137, 436, 545, 923, 1097
Hpy8I GTNNAC 2 cut(s) 700, 1072
HpyCH4III ACNGT 3 cut(s) 356, 433, 529
HpyCH4IV ACGT 3 cut(s) 492, 702, 1059
HpyCH4V TGCA 5 cut(s) 38, 283, 574, 894, 904
HpyF3I CTNAG 8 cut(s) 254, 348, 416, 669, 708, 876, 908, 1010
HpySE526I ACGT 3 cut(s) 492, 702, 1059
Hsp92II CATG 3 cut(s) 268, 463, 569
HspAI GCGC 1 cut(s) 362
KspAI GTTAAC 1 cut(s) 1072
Kzo9I GATC 4 cut(s) 471, 925, 1025, 1085
LguI GCTCTTC 1 cut(s) 999
LmnI GCTCC 3 cut(s) 188, 445, 944
Lsp1109I GCAGC 3 cut(s) 281, 586, 822
MaeI CTAG 2 cut(s) 87, 996
MaeII ACGT 3 cut(s) 492, 702, 1059
MaeIII GTNAC 5 cut(s) 260, 340, 383, 985, 998
MalI GATC 4 cut(s) 473, 927, 1027, 1087
MboI GATC 4 cut(s) 471, 925, 1025, 1085
MflI RGATCY 1 cut(s) 1085
MhlI GDGCHC 1 cut(s) 877
MluCI AATT 5 cut(s) 46, 325, 531, 652, 845
MlyI GAGTC 1 cut(s) 610
MmeI TCCRAC 2 cut(s) 384, 584
MseI TTAA 1 cut(s) 1071
MslI CAYNNNNRTG 3 cut(s) 128, 240, 464
MspI CCGG 1 cut(s) 578
MspR9I CCNGG 2 cut(s) 443, 579
MvaI CCWGG 1 cut(s) 443
NciI CCSGG 1 cut(s) 579
NdeII GATC 4 cut(s) 471, 925, 1025, 1085
NlaIII CATG 3 cut(s) 268, 463, 569
NlaIV GGNNCC 1 cut(s) 447
NmuCI GTSAC 2 cut(s) 340, 985
PciSI GCTCTTC 1 cut(s) 999
PfeI GAWTC 1 cut(s) 1034
PkrI GCNGC 3 cut(s) 271, 576, 812
PleI GAGTC 1 cut(s) 609
PpsI GAGTC 1 cut(s) 609
Ppu21I YACGTR 1 cut(s) 1060
Psp6I CCWGG 1 cut(s) 441
PspGI CCWGG 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 447
PspPI GGNCC 1 cut(s) 827
PsuI RGATCY 1 cut(s) 1085
RsaI GTAC 3 cut(s) 353, 495, 962
RsaNI GTAC 3 cut(s) 352, 494, 961
RseI CAYNNNNRTG 3 cut(s) 128, 240, 464
SapI GCTCTTC 1 cut(s) 999
SaqAI TTAA 1 cut(s) 1071
SatI GCNGC 3 cut(s) 270, 575, 811
Sau3AI GATC 4 cut(s) 471, 925, 1025, 1085
Sau96I GGNCC 1 cut(s) 827
ScaI AGTACT 1 cut(s) 353
SchI GAGTC 1 cut(s) 610
ScrFI CCNGG 2 cut(s) 443, 579
SduI GDGCHC 1 cut(s) 877
SmiMI CAYNNNNRTG 3 cut(s) 128, 240, 464
SmlI CTYRAG 2 cut(s) 135, 497
SmoI CTYRAG 2 cut(s) 135, 497
SnaBI TACGTA 1 cut(s) 1060
SpeI ACTAGT 1 cut(s) 995
Sse9I AATT 5 cut(s) 46, 325, 531, 652, 845
SspMI CTAG 2 cut(s) 87, 996
StyD4I CCNGG 2 cut(s) 441, 577
TaaI ACNGT 3 cut(s) 356, 433, 529
TaiI ACGT 3 cut(s) 495, 705, 1062
TaqI TCGA 4 cut(s) 240, 762, 1096, 1111
TasI AATT 5 cut(s) 46, 325, 531, 652, 845
TatI WGTACW 2 cut(s) 351, 960
TfiI GAWTC 1 cut(s) 1034
Tru1I TTAA 1 cut(s) 1071
Tru9I TTAA 1 cut(s) 1071
TscAI CASTG 2 cut(s) 106, 436
TseFI GTSAC 2 cut(s) 340, 985
TseI GCWGC 3 cut(s) 269, 574, 810
Tsp45I GTSAC 2 cut(s) 340, 985
TspDTI ATGAA 3 cut(s) 112, 605, 653
TspRI CASTG 2 cut(s) 106, 436
XagI CCTNNNNNAGG 1 cut(s) 253
XapI RAATTY 2 cut(s) 46, 652
XspI CTAG 2 cut(s) 87, 996
ZrmI AGTACT 1 cut(s) 353
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.