Rroxscaffold_2G00140140

PB1 domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0012555
Physical Location & Seq
Reverse (-)
78406993 .. 78411018
4026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00140140.1

Sequence Viewer

Length: 939 bp
ATGATCGACGACGAAGGAATGAAGCTCGAAGGCTGCAATGGTTTTCCGGCAGTTCGGCAAAGTGGGAAGAAGAAAAGAAGTGCAGGTGATAGTCCAAAGCCAAGCAACAGTAGCAAAAGTAGTCCAAAAAACCAAGATAAGGATACAGAAGTTTTCATTGCAATGGCTCATGAATTGAAGGATGAGGGGAATAACTTGTTTCAGAAAAGGGACCATGAAGGAGCTATGTTCAAGTATGAAAAAGCCCTCAGATTGCTTCCGAGAAATCACATCTATGTGTCCTATCTTCGGAGTAATGTGGCGGCTTGTTATATGCAGATGGGGCTGAGTGAGTATCCGAGGGCTATTAATGAGTGCAATTTGGCCCTTGAAGTTACACCTAATTATAGTAAGGCTCTGCTGAAGAGGGCAAAGTGCTATGAGGCTTTGAATAGGTTGGATTTGGCTCTAAGAGATGTTGGTACCGTGTTGAACACGGAACCAAACAATATCATGGCACTGGAGGTTGCAGAAAGAGTGAAAGATGTGCTAGAGAAGAAGGGATTGAGGGTGAATGACACGGTGATTGAGTTGCCTCCTGATTATGTAGAACCAAGTCATAATGTGCTGCCAATGAAATTAACGAAATCGAAGAGTGGGAAGAATAAAGGGAACAAAGATGAAGAGAAGAAAGTTCATGACGACAATACCGGGGGGAATACTGAAGACAAGATTGAGGAGAAGAAGGCTGTGGAAATAGGTGAGGAAAAGAAGTCTGTGGGTATAAGTGAGGAAGATAAGGATGTGGTAATAGCTGAGGAAAAGAAGGCAGAGGTCATGGTAGTTGTGGAAGAGAAGATCACAACAGCGGAAGAGGTGCCGAAAAGAAATGTGAAATTGGTGTTGGGAGAGGACATAAGATGGGTCAGTTGCCAGTATATTGCACCCTCCTGCAACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PB1

Protein Analysis

312

Amino Acids

35.0

Weight (kDa)

6.09

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 74
Acc36I ACCTGC 1 cut(s) 74
Acc65I GGTACC 1 cut(s) 461
AccB1I GGYRCC 2 cut(s) 461, 856
AciI CCGC 2 cut(s) 302, 848
AcuI CTGAAG 2 cut(s) 422, 723
AfaI GTAC 1 cut(s) 463
AfiI CCNNNNNNNGG 2 cut(s) 139, 288
AgsI TTSAA 5 cut(s) 178, 232, 371, 430, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 866, 898
AluBI AGCT 3 cut(s) 25, 224, 794
AluI AGCT 3 cut(s) 25, 224, 794
AoxI GGCC 1 cut(s) 363
ApeKI GCWGC 2 cut(s) 33, 607
AseI ATTAAT 1 cut(s) 348
Asp718I GGTACC 1 cut(s) 461
AspS9I GGNCC 2 cut(s) 211, 364
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 4 cut(s) 98, 562, 574, 752
AvaII GGWCC 1 cut(s) 211
BaeI ACNNNNGTAYC 2 cut(s) 135, 168
BanI GGYRCC 2 cut(s) 461, 856
BbsI GAAGAC 1 cut(s) 711
BbvCI CCTCAGC 1 cut(s) 795
BbvI GCAGC 2 cut(s) 20, 594
BccI CCATC 2 cut(s) 313, 894
BciVI GTATCC 2 cut(s) 136, 345
BcnI CCSGG 1 cut(s) 691
BfaI CTAG 1 cut(s) 530
BfuAI ACCTGC 1 cut(s) 74
BfuI GTATCC 2 cut(s) 136, 345
BisI GCNGC 3 cut(s) 34, 303, 608
BlsI GCNGC 3 cut(s) 35, 304, 609
Bme1390I CCNGG 1 cut(s) 691
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 2 cut(s) 211, 364
BmiI GGNNCC 4 cut(s) 212, 463, 480, 858
BmrFI CCNGG 1 cut(s) 691
BpiI GAAGAC 1 cut(s) 711
BpmI CTGGAG 1 cut(s) 521
Bpu10I CCTNAGC 1 cut(s) 795
BpuMI CCSGG 1 cut(s) 691
BsaJI CCNNGG 2 cut(s) 338, 690
Bsc4I CCNNNNNNNGG 2 cut(s) 139, 288
Bse1I ACTGG 2 cut(s) 504, 913
Bse3DI GCAATG 3 cut(s) 43, 156, 168
BseDI CCNNGG 2 cut(s) 338, 690
BseGI GGATG 2 cut(s) 187, 787
BseLI CCNNNNNNNGG 2 cut(s) 139, 288
BseMI GCAATG 3 cut(s) 43, 156, 168
BseMII CTCAG 3 cut(s) 262, 317, 786
BseNI ACTGG 2 cut(s) 504, 913
BseRI GAGGAG 1 cut(s) 731
BseXI GCAGC 2 cut(s) 20, 594
BsgI GTGCAG 1 cut(s) 102
BshFI GGCC 1 cut(s) 365
BshNI GGYRCC 2 cut(s) 461, 856
BsiSI CCGG 2 cut(s) 47, 690
BslFI GGGAC 1 cut(s) 224
BslI CCNNNNNNNGG 2 cut(s) 139, 288
BsmFI GGGAC 1 cut(s) 224
BsnI GGCC 1 cut(s) 365
Bsp143I GATC 2 cut(s) 3, 837
BspACI CCGC 2 cut(s) 302, 848
BspANI GGCC 1 cut(s) 365
BspCNI CTCAG 3 cut(s) 261, 318, 787
BspHI TCATGA 2 cut(s) 169, 676
BspLI GGNNCC 4 cut(s) 212, 463, 480, 858
BspMI ACCTGC 1 cut(s) 74
BspT107I GGYRCC 2 cut(s) 461, 856
BsrDI GCAATG 3 cut(s) 43, 156, 168
BsrI ACTGG 2 cut(s) 504, 913
BssECI CCNNGG 2 cut(s) 338, 690
BssMI GATC 2 cut(s) 3, 837
Bst4CI ACNGT 3 cut(s) 110, 466, 562
Bst6I CTCTTC 5 cut(s) 398, 626, 657, 825, 846
BstDEI CTNAG 4 cut(s) 248, 326, 449, 795
BstF5I GGATG 2 cut(s) 187, 787
BstKTI GATC 2 cut(s) 6, 840
BstMBI GATC 2 cut(s) 3, 837
BstMWI GCNNNNNNNGC 2 cut(s) 111, 322
BstSCI CCNGG 1 cut(s) 689
BstV1I GCAGC 2 cut(s) 20, 594
BstV2I GAAGAC 1 cut(s) 711
BsuI GTATCC 2 cut(s) 136, 345
BsuRI GGCC 1 cut(s) 365
BtsCI GGATG 2 cut(s) 187, 787
BtsIMutI CAGTG 1 cut(s) 497
BveI ACCTGC 1 cut(s) 74
CciI TCATGA 2 cut(s) 169, 676
Cfr13I GGNCC 2 cut(s) 211, 364
Csp6I GTAC 1 cut(s) 462
CviAII CATG 5 cut(s) 170, 215, 493, 677, 817
CviQI GTAC 1 cut(s) 462
DdeI CTNAG 4 cut(s) 248, 326, 449, 795
DpnI GATC 2 cut(s) 5, 839
DpnII GATC 2 cut(s) 3, 837
Eam1104I CTCTTC 5 cut(s) 398, 626, 657, 825, 846
EarI CTCTTC 5 cut(s) 398, 626, 657, 825, 846
Eco47I GGWCC 1 cut(s) 211
Eco57I CTGAAG 2 cut(s) 422, 723
FaeI CATG 5 cut(s) 173, 218, 496, 680, 820
FaqI GGGAC 1 cut(s) 224
FatI CATG 5 cut(s) 169, 214, 492, 676, 816
Fnu4HI GCNGC 3 cut(s) 34, 303, 608
FokI GGATG 2 cut(s) 194, 794
Fsp4HI GCNGC 3 cut(s) 34, 303, 608
FspBI CTAG 1 cut(s) 530
GluI GCNGC 3 cut(s) 34, 303, 608
GsuI CTGGAG 1 cut(s) 521
HaeIII GGCC 1 cut(s) 365
HapII CCGG 2 cut(s) 47, 690
Hin1II CATG 5 cut(s) 173, 218, 496, 680, 820
HpaII CCGG 2 cut(s) 47, 690
HphI GGTGA 4 cut(s) 98, 562, 574, 752
Hpy188I TCNGA 5 cut(s) 204, 251, 261, 291, 339
Hpy188III TCNNGA 3 cut(s) 170, 578, 677
Hpy99I CGWCG 2 cut(s) 11, 14
HpyAV CCTTC 7 cut(s) 8, 23, 172, 212, 532, 718, 799
HpyCH4III ACNGT 3 cut(s) 110, 466, 562
HpyCH4V TGCA 8 cut(s) 36, 83, 161, 316, 357, 509, 923, 933
HpyF10VI GCNNNNNNNGC 2 cut(s) 111, 322
HpyF3I CTNAG 4 cut(s) 248, 326, 449, 795
Hsp92II CATG 5 cut(s) 173, 218, 496, 680, 820
KpnI GGTACC 1 cut(s) 465
Kzo9I GATC 2 cut(s) 3, 837
LmnI GCTCC 1 cut(s) 221
LpnPI CCDG 6 cut(s) 60, 69, 485, 591, 703, 926
Lsp1109I GCAGC 2 cut(s) 20, 594
MaeI CTAG 1 cut(s) 530
MaeIII GTNAC 1 cut(s) 373
MalI GATC 2 cut(s) 5, 839
MboI GATC 2 cut(s) 3, 837
MluCI AATT 5 cut(s) 173, 358, 382, 617, 875
MmeI TCCRAC 1 cut(s) 417
MseI TTAA 2 cut(s) 348, 620
MslI CAYNNNNRTG 3 cut(s) 161, 273, 275
MspA1I CMGCKG 1 cut(s) 848
MspI CCGG 2 cut(s) 47, 690
MspR9I CCNGG 1 cut(s) 691
MwoI GCNNNNNNNGC 2 cut(s) 111, 322
NciI CCSGG 1 cut(s) 691
NdeII GATC 2 cut(s) 3, 837
NlaIII CATG 5 cut(s) 173, 218, 496, 680, 820
NlaIV GGNNCC 4 cut(s) 212, 463, 480, 858
PagI TCATGA 2 cut(s) 169, 676
PaqCI CACCTGC 1 cut(s) 74
PkrI GCNGC 3 cut(s) 35, 304, 609
PshBI ATTAAT 1 cut(s) 348
PspN4I GGNNCC 4 cut(s) 212, 463, 480, 858
PspPI GGNCC 2 cut(s) 211, 364
RsaI GTAC 1 cut(s) 463
RsaNI GTAC 1 cut(s) 462
RseI CAYNNNNRTG 3 cut(s) 161, 273, 275
SaqAI TTAA 2 cut(s) 348, 620
SatI GCNGC 3 cut(s) 34, 303, 608
Sau3AI GATC 2 cut(s) 3, 837
Sau96I GGNCC 2 cut(s) 211, 364
ScrFI CCNGG 1 cut(s) 691
SinI GGWCC 1 cut(s) 211
SmiMI CAYNNNNRTG 3 cut(s) 161, 273, 275
Sse9I AATT 5 cut(s) 173, 358, 382, 617, 875
SsiI CCGC 2 cut(s) 302, 848
SspMI CTAG 1 cut(s) 530
StyD4I CCNGG 1 cut(s) 689
TaaI ACNGT 3 cut(s) 110, 466, 562
TaqI TCGA 3 cut(s) 6, 27, 629
TasI AATT 5 cut(s) 173, 358, 382, 617, 875
TauI GCSGC 1 cut(s) 305
Tru1I TTAA 2 cut(s) 348, 620
Tru9I TTAA 2 cut(s) 348, 620
TscAI CASTG 1 cut(s) 504
TseI GCWGC 2 cut(s) 33, 607
TspDTI ATGAA 8 cut(s) 35, 145, 186, 231, 252, 629, 665, 675
TspGWI ACGGA 1 cut(s) 491
TspRI CASTG 1 cut(s) 504
VpaK11BI GGWCC 1 cut(s) 211
VspI ATTAAT 1 cut(s) 348
XspI CTAG 1 cut(s) 530
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.