FvH4_1g02110

anion transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0009581
Physical Location & Seq
Reverse (-)
1115396 .. 1122629
7234 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02110.t4

Sequence Viewer

Length: 1551 bp
ATGGCCAAACTCACTCTCCGAGCAGAAAGCTCCCACCTTTTCTACTCACAGCCCTCATCTTTCATCTCAGCACCGAAACGCCGTCGTTTGCTGAGCTTCCCTCCGAAACCCAGCTTGAAATTCCGAGTCTTTTGCAGCATTAAGGAGAAGGAGAGCGTTAAGAGAGTCAATGGGCTCCCCGGCGATGAGGTGGTCCACCGGGCCGGGTCGGGTTCGGATTCTGAAGGCGAGTCGGGTCAGAAAAGTGGGTCTGTGGGGTTTGATTTGGACTGGCCTCCCTGGAAGAACATACCTCAGAGATATAAGCTCATTGGCACAACTTCATTGGCCTTTGTTATTTGTAACATGGATAAGGTGAACTTGAGTATTGCTATAATCCCAATGTCACATCAGTTTGGTTGGAGTTCATCTGTGGCTGGATTGGTTCAGTCGTCCTTCTTTTGGGGGTATGCTCTGAGTCAATTGCCTGGGGGATGGCTTGCCAAGATATTTGGTGGGAGAAAAGTGCTTGAGATTGGAGTATTAACTTGGTCATTGGCTACAGCACTTGTCCCTTTCGTTGCTGGATTTACTCCTGGTTTAGTTGTGTCAAGAATTTTGGTAGGAATTGGAGAAGGTGTTTCCCCGTCCGCTGCAACTGACCTTATTGCCAGAACAATACCTTTGGAAGAGCGGTCACGAGCCGTAGCATTTGTCTTTGGTGGGTTGAGTGTAGGCAGTGTTGCAGGGTTTCTTCTAGCTCCTCCACTTATCCAAAATCTTGGCTGGGAATCTGTCTTTTACATATTTTCCCTTTTGGGGATAGGTTGGTATGTGGGGTTTCAGTTTCTTGAAGGACAACCCTCATGGGTTGGGTCCCGGTCAGACTTGAAAACAGCGCAGAACACTTCTCCAAAAGAGTTGGCTGACTCGTTGAAGGATGTACCGTGGAAGGAATTTTTCCGAAGTGAAGCTGTTTGGGCAATGATATATGCTCATTTTTGTGGAAGTTGGGGTCATTACACATGCCTAGCATGGCTGCCTACTTATTTTAGTGAGGAGCTGAACCTGAATTTGACCGAAGCGGCCTGGGTCTCTATCCTTCCTCCCTTGGCATCAATATTTGTGAATACCATTGCATCCCAAATTGCTGATAACTTGATTTCTAATGGAGTTCAAACCACTACGGTCCGAAAAATTTGCCAAACAATTGCCTTTTTGTCTCCTGCAGCTTGTATGACTCTTTCTTCTCTTGATCTAGGATTGCCCCATTGGGAAGTTGTGGGGATACTCACTGCTGGTTTAGCCCTTTCGAGCTTTGCAATTTCAGGACTTTATTGTACTCATCAAGATATATCACCTGAATATGCAAGCATACTTTTGGGTATTACCAATACTGTTGGGGCAGTACCTGGAATAGTAGGTGTTGCCCTCACTGGCTATCTACTTGATTCAACTCATTCATGGAGTATGTCATTGTTCATTCCATCAATATTTTTCTACCTGACTGGTACAATTGTTTGGTTGGCGTTTGCCAGCAGTAAGCCTCAGACCTTTGCGAAGAGAGATTGA

Protein Analysis

517

Amino Acids

55.96

Weight (kDa)

8.37

Isoelectric Point (pI)

45.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 108 - 468 1.3e-52 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 673
AciI CCGC 3 cut(s) 630, 673, 1064
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 5 cut(s) 119, 594, 935, 1051, 1176
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 4 cut(s) 924, 1321, 1389, 1492
AfiI CCNNNNNNNGG 2 cut(s) 441, 798
AgsI TTSAA 6 cut(s) 118, 833, 871, 916, 1157, 1434
AjnI CCWGG 5 cut(s) 278, 466, 574, 1067, 1390
AluBI AGCT 9 cut(s) 30, 96, 114, 307, 740, 953, 1042, 1211, 1296
AluI AGCT 9 cut(s) 30, 96, 114, 307, 740, 953, 1042, 1211, 1296
Alw26I GTCTC 2 cut(s) 1078, 1206
AlwNI CAGNNNCTG 1 cut(s) 1391
AoxI GGCC 5 cut(s) 3, 201, 272, 327, 1065
ApeKI GCWGC 4 cut(s) 135, 632, 1018, 1208
ApoI RAATTY 5 cut(s) 119, 594, 935, 1051, 1176
AspLEI GCGC 1 cut(s) 880
AspS9I GGNCC 4 cut(s) 193, 201, 855, 1168
AsuC2I CCSGG 4 cut(s) 180, 200, 205, 859
AsuHPI GGTGA 2 cut(s) 367, 1329
AvaII GGWCC 3 cut(s) 193, 855, 1168
BaeI ACNNNNGTAYC 2 cut(s) 1482, 1515
BalI TGGCCA 1 cut(s) 5
BanII GRGCYC 1 cut(s) 177
BauI CACGAG 1 cut(s) 678
BbvI GCAGC 4 cut(s) 147, 619, 1005, 1220
BccI CCATC 2 cut(s) 468, 1474
BceAI ACGGC 2 cut(s) 66, 668
BcgI CGANNNNNNTGC 4 cut(s) 114, 148, 1161, 1195
BciT130I CCWGG 5 cut(s) 280, 468, 576, 1069, 1392
BciVI GTATCC 1 cut(s) 1260
BcnI CCSGG 4 cut(s) 180, 200, 205, 859
BcoDI GTCTC 2 cut(s) 1078, 1206
BfaI CTAG 3 cut(s) 737, 1010, 1238
BfmI CTRYAG 2 cut(s) 540, 1206
BfuI GTATCC 1 cut(s) 1260
BisI GCNGC 5 cut(s) 136, 633, 1019, 1065, 1209
BlpI GCTNAGC 1 cut(s) 92
BlsI GCNGC 5 cut(s) 137, 634, 1020, 1066, 1210
Bme1390I CCNGG 9 cut(s) 180, 200, 205, 280, 468, 576, 859, 1069, 1392
Bme18I GGWCC 3 cut(s) 193, 855, 1168
BmgT120I GGNCC 4 cut(s) 193, 201, 855, 1168
BmiI GGNNCC 3 cut(s) 176, 856, 857
BmrFI CCNGG 9 cut(s) 180, 200, 205, 280, 468, 576, 859, 1069, 1392
BmsI GCATC 2 cut(s) 1103, 1127
BplI GAGNNNNNCTC 2 cut(s) 85, 117
Bpu1102I GCTNAGC 1 cut(s) 92
BpuEI CTTGAG 2 cut(s) 382, 530
BpuMI CCSGG 4 cut(s) 180, 200, 205, 859
BsaI GGTCTC 1 cut(s) 1078
BsaJI CCNNGG 6 cut(s) 178, 278, 467, 926, 1068, 1089
BsaXI ACNNNNNCTCC 3 cut(s) 30, 603, 633
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 798
Bse1I ACTGG 3 cut(s) 275, 1420, 1492
Bse3DI GCAATG 2 cut(s) 969, 1113
BseBI CCWGG 5 cut(s) 280, 468, 576, 1069, 1392
BseDI CCNNGG 6 cut(s) 178, 278, 467, 926, 1068, 1089
BseGI GGATG 3 cut(s) 479, 925, 1118
BseLI CCNNNNNNNGG 2 cut(s) 441, 798
BseMI GCAATG 2 cut(s) 969, 1113
BseMII CTCAG 5 cut(s) 81, 83, 308, 446, 1541
BseNI ACTGG 3 cut(s) 275, 1420, 1492
BseRI GAGGAG 2 cut(s) 732, 1052
BseXI GCAGC 4 cut(s) 147, 619, 1005, 1220
BseYI CCCAGC 2 cut(s) 110, 765
BshFI GGCC 5 cut(s) 5, 203, 274, 329, 1067
BsiSI CCGG 4 cut(s) 180, 199, 204, 859
BslFI GGGAC 2 cut(s) 536, 841
BslI CCNNNNNNNGG 2 cut(s) 441, 798
BsmAI GTCTC 2 cut(s) 1078, 1206
BsmFI GGGAC 2 cut(s) 536, 841
BsnI GGCC 5 cut(s) 5, 203, 274, 329, 1067
Bso31I GGTCTC 1 cut(s) 1078
Bsp1286I GDGCHC 1 cut(s) 177
Bsp143I GATC 1 cut(s) 1234
Bsp1720I GCTNAGC 1 cut(s) 92
BspACI CCGC 3 cut(s) 630, 673, 1064
BspANI GGCC 5 cut(s) 5, 203, 274, 329, 1067
BspCNI CTCAG 5 cut(s) 80, 84, 307, 447, 1540
BspLI GGNNCC 3 cut(s) 176, 856, 857
BspMAI CTGCAG 1 cut(s) 1210
BspQI GCTCTTC 1 cut(s) 663
BspTNI GGTCTC 1 cut(s) 1078
BsrBI CCGCTC 1 cut(s) 673
BsrDI GCAATG 2 cut(s) 969, 1113
BsrI ACTGG 3 cut(s) 275, 1420, 1492
BssECI CCNNGG 6 cut(s) 178, 278, 467, 926, 1068, 1089
BssMI GATC 1 cut(s) 1234
BssSI CACGAG 1 cut(s) 678
BssT1I CCWWGG 1 cut(s) 1089
Bst2BI CACGAG 1 cut(s) 678
Bst2UI CCWGG 5 cut(s) 280, 468, 576, 1069, 1392
Bst4CI ACNGT 3 cut(s) 927, 1168, 1378
Bst6I CTCTTC 2 cut(s) 663, 1535
BstC8I GCNNGC 3 cut(s) 480, 1351, 1516
BstDEI CTNAG 5 cut(s) 67, 92, 294, 455, 1527
BstDSI CCRYGG 1 cut(s) 926
BstF5I GGATG 3 cut(s) 479, 925, 1118
BstHHI GCGC 1 cut(s) 880
BstKTI GATC 1 cut(s) 1237
BstMAI GTCTC 2 cut(s) 1078, 1206
BstMBI GATC 1 cut(s) 1234
BstMWI GCNNNNNNNGC 2 cut(s) 959, 1283
BstNI CCWGG 5 cut(s) 280, 468, 576, 1069, 1392
BstNSI RCATGY 1 cut(s) 1008
BstSCI CCNGG 9 cut(s) 178, 198, 203, 278, 466, 574, 857, 1067, 1390
BstSFI CTRYAG 2 cut(s) 540, 1206
BstV1I GCAGC 4 cut(s) 147, 619, 1005, 1220
BstXI CCANNNNNNTGG 1 cut(s) 761
BsuI GTATCC 1 cut(s) 1260
BsuRI GGCC 5 cut(s) 5, 203, 274, 329, 1067
BtgI CCRYGG 1 cut(s) 926
BtgZI GCGATG 1 cut(s) 198
BtsCI GGATG 3 cut(s) 479, 925, 1118
BtsI GCAGTG 2 cut(s) 724, 1272
BtsIMutI CAGTG 3 cut(s) 724, 1272, 1413
Cac8I GCNNGC 3 cut(s) 480, 1351, 1516
CaiI CAGNNNCTG 1 cut(s) 1391
CfoI GCGC 1 cut(s) 880
Cfr13I GGNCC 4 cut(s) 193, 201, 855, 1168
CpoI CGGWCCG 1 cut(s) 1168
Csp6I GTAC 4 cut(s) 923, 1320, 1388, 1491
CspI CGGWCCG 1 cut(s) 1168
CviAII CATG 5 cut(s) 346, 846, 1005, 1014, 1443
CviQI GTAC 4 cut(s) 923, 1320, 1388, 1491
DdeI CTNAG 5 cut(s) 67, 92, 294, 455, 1527
DpnI GATC 1 cut(s) 1236
DpnII GATC 1 cut(s) 1234
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 663, 1535
EarI CTCTTC 2 cut(s) 663, 1535
Eco130I CCWWGG 1 cut(s) 1089
Eco24I GRGCYC 1 cut(s) 177
Eco31I GGTCTC 1 cut(s) 1078
Eco47I GGWCC 3 cut(s) 193, 855, 1168
Eco57I CTGAAG 1 cut(s) 243
EcoO109I RGGNCCY 1 cut(s) 855
EcoRII CCWGG 5 cut(s) 278, 466, 574, 1067, 1390
EcoT14I CCWWGG 1 cut(s) 1089
EcoT38I GRGCYC 1 cut(s) 177
ErhI CCWWGG 1 cut(s) 1089
FaeI CATG 5 cut(s) 349, 849, 1008, 1017, 1446
FalI AAGNNNNNCTT 2 cut(s) 344, 376
FaqI GGGAC 2 cut(s) 536, 841
FatI CATG 5 cut(s) 345, 845, 1004, 1013, 1442
Fnu4HI GCNGC 5 cut(s) 136, 633, 1019, 1065, 1209
FokI GGATG 3 cut(s) 486, 932, 1105
FriOI GRGCYC 1 cut(s) 177
Fsp4HI GCNGC 5 cut(s) 136, 633, 1019, 1065, 1209
FspBI CTAG 3 cut(s) 737, 1010, 1238
GlaI GCGC 1 cut(s) 879
GluI GCNGC 5 cut(s) 136, 633, 1019, 1065, 1209
GsaI CCCAGC 2 cut(s) 114, 769
HaeIII GGCC 5 cut(s) 5, 203, 274, 329, 1067
HapII CCGG 4 cut(s) 180, 199, 204, 859
HhaI GCGC 1 cut(s) 880
Hin1II CATG 5 cut(s) 349, 849, 1008, 1017, 1446
Hin6I GCGC 1 cut(s) 878
HinP1I GCGC 1 cut(s) 878
HinfI GANTC 9 cut(s) 126, 165, 218, 230, 457, 770, 908, 1219, 1430
HpaII CCGG 4 cut(s) 180, 199, 204, 859
HphI GGTGA 2 cut(s) 367, 1329
Hpy166II GTNNAC 2 cut(s) 196, 358
Hpy188III TCNNGA 6 cut(s) 591, 678, 830, 1232, 1308, 1328
Hpy8I GTNNAC 2 cut(s) 196, 358
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 8 cut(s) 142, 218, 445, 608, 827, 910, 925, 1091
HpyCH4III ACNGT 3 cut(s) 927, 1168, 1378
HpyCH4V TGCA 7 cut(s) 135, 635, 725, 1118, 1208, 1301, 1349
HpyF10VI GCNNNNNNNGC 2 cut(s) 959, 1283
HpyF3I CTNAG 5 cut(s) 67, 92, 294, 455, 1527
Hsp92II CATG 5 cut(s) 349, 849, 1008, 1017, 1446
HspAI GCGC 1 cut(s) 878
KflI GGGWCCC 1 cut(s) 855
Kzo9I GATC 1 cut(s) 1234
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 4 cut(s) 35, 180, 745, 1039
Lsp1109I GCAGC 4 cut(s) 147, 619, 1005, 1220
LweI GCATC 2 cut(s) 1103, 1127
MaeI CTAG 3 cut(s) 737, 1010, 1238
MaeIII GTNAC 3 cut(s) 341, 384, 675
MalI GATC 1 cut(s) 1236
MbiI CCGCTC 1 cut(s) 673
MboI GATC 1 cut(s) 1234
MboII GAAGA 4 cut(s) 295, 680, 725, 1218
MfeI CAATTG 3 cut(s) 461, 1188, 1494
MhlI GDGCHC 1 cut(s) 177
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 6 cut(s) 135, 174, 239, 466, 902, 1213
MmeI TCCRAC 1 cut(s) 380
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 141, 159, 524
MslI CAYNNNNRTG 1 cut(s) 981
Msp20I TGGCCA 1 cut(s) 5
MspA1I CMGCKG 1 cut(s) 632
MspI CCGG 4 cut(s) 180, 199, 204, 859
MspR9I CCNGG 9 cut(s) 180, 200, 205, 280, 468, 576, 859, 1069, 1392
MunI CAATTG 3 cut(s) 461, 1188, 1494
MvaI CCWGG 5 cut(s) 280, 468, 576, 1069, 1392
MwoI GCNNNNNNNGC 2 cut(s) 959, 1283
NciI CCSGG 4 cut(s) 180, 200, 205, 859
NdeII GATC 1 cut(s) 1234
NlaIII CATG 5 cut(s) 349, 849, 1008, 1017, 1446
NlaIV GGNNCC 3 cut(s) 176, 856, 857
NmuCI GTSAC 2 cut(s) 384, 675
NspI RCATGY 1 cut(s) 1008
PciSI GCTCTTC 1 cut(s) 663
PfeI GAWTC 3 cut(s) 218, 770, 1430
PkrI GCNGC 5 cut(s) 137, 634, 1020, 1066, 1210
PleI GAGTC 6 cut(s) 134, 173, 238, 465, 902, 1213
PpsI GAGTC 6 cut(s) 134, 173, 238, 465, 902, 1213
PpuMI RGGWCCY 1 cut(s) 855
Psp5II RGGWCCY 1 cut(s) 855
Psp6I CCWGG 5 cut(s) 278, 466, 574, 1067, 1390
PspFI CCCAGC 2 cut(s) 110, 765
PspGI CCWGG 5 cut(s) 278, 466, 574, 1067, 1390
PspN4I GGNNCC 3 cut(s) 176, 856, 857
PspPI GGNCC 4 cut(s) 193, 201, 855, 1168
PspPPI RGGWCCY 1 cut(s) 855
PstI CTGCAG 1 cut(s) 1210
PstNI CAGNNNCTG 1 cut(s) 1391
RsaI GTAC 4 cut(s) 924, 1321, 1389, 1492
RsaNI GTAC 4 cut(s) 923, 1320, 1388, 1491
RseI CAYNNNNRTG 1 cut(s) 981
Rsr2I CGGWCCG 1 cut(s) 1168
RsrII CGGWCCG 1 cut(s) 1168
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 3 cut(s) 141, 159, 524
SatI GCNGC 5 cut(s) 136, 633, 1019, 1065, 1209
Sau3AI GATC 1 cut(s) 1234
Sau96I GGNCC 4 cut(s) 193, 201, 855, 1168
SchI GAGTC 6 cut(s) 135, 174, 239, 466, 902, 1213
ScrFI CCNGG 9 cut(s) 180, 200, 205, 280, 468, 576, 859, 1069, 1392
SduI GDGCHC 1 cut(s) 177
SfaNI GCATC 2 cut(s) 1103, 1127
SfcI CTRYAG 2 cut(s) 540, 1206
SinI GGWCC 3 cut(s) 193, 855, 1168
SmiMI CAYNNNNRTG 1 cut(s) 981
SmlI CTYRAG 2 cut(s) 361, 509
SmoI CTYRAG 2 cut(s) 361, 509
SsiI CCGC 3 cut(s) 630, 673, 1064
SspI AATATT 2 cut(s) 1101, 1473
SspMI CTAG 3 cut(s) 737, 1010, 1238
StyD4I CCNGG 9 cut(s) 178, 198, 203, 278, 466, 574, 857, 1067, 1390
StyI CCWWGG 1 cut(s) 1089
TaaI ACNGT 3 cut(s) 927, 1168, 1378
TaqI TCGA 1 cut(s) 1292
TaqII GACCGA 1 cut(s) 1073
TatI WGTACW 1 cut(s) 1319
TauI GCSGC 1 cut(s) 1067
TfiI GAWTC 3 cut(s) 218, 770, 1430
Tru1I TTAA 3 cut(s) 141, 159, 524
Tru9I TTAA 3 cut(s) 141, 159, 524
TscAI CASTG 3 cut(s) 724, 1279, 1420
TseFI GTSAC 2 cut(s) 384, 675
TseI GCWGC 4 cut(s) 135, 632, 1018, 1208
Tsp45I GTSAC 2 cut(s) 384, 675
TspDTI ATGAA 5 cut(s) 52, 312, 396, 1431, 1450
TspRI CASTG 3 cut(s) 724, 1279, 1420
VpaK11BI GGWCC 3 cut(s) 193, 855, 1168
XapI RAATTY 5 cut(s) 119, 594, 935, 1051, 1176
XceI RCATGY 1 cut(s) 1008
XspI CTAG 3 cut(s) 737, 1010, 1238
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.