Rroxscaffold_2G00153830

anion transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0009581
Physical Location & Seq
Forward (+)
90548978 .. 90555583
6606 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00153830.1

Sequence Viewer

Length: 1617 bp
ATGGCCAAACTCACTCTCCGAGCAGAAAGCTCCTACCTTTTTTCCCACACAACCTCCGCCTCCCACTCCCAGCCCTCTTCTTTCACCTCAGCACCGAAACGCCGTCGTTTGCTGAGCTTCCCTTCGAAGCACAGCTTGAAATTCAGAGTCTTTTGCAGCATTAAGGAGAAGGAGAGCGTCAAGAGGGTCAATGGGCTCCCCGCCGGAGATAAGGTCCACCGGGTCGGGTCGGGTTCGGATTCTGAAGGCGAGTCGGGTCTGGAAAGTGGGTCTGGGGCTGTGGGGTTTGATTTGGACTGGCCTCCGTGGAAGAACATACCCCAGAGATATAAGCTCATTGGCACAACTTCATTGGCTTTTGTTATCTGTAACATGGATAAGGTGAACTTGAGTATTGCTATAATTCCCATGTCGCATCAGTTTGGTTGGAGTTCATCAGTGGCTGGATTGGTTCAGTCGTCCTTCTTTTGGGGGTATGCTTTGAGTCAATTGCCTGGGGGCTGGCTTGCCAAGATATTTGGTGGGAGAAAAGTTCTTGAGATTGGAGTATTAACTTGGTCATTGGCTACAGCACTTGTTCCTTTCGTTGCTGGATTTACTCCTGGTTTAGTTGTGTCAAGAATTTTGGTAGGGATTGGAGAAGGTGTTTCCCCGTCTGCTGCAACTGACCTTATTGCCAGGCATAACTCAACCGTTAAACTCACTTTTATTTCATGCAGAACAATACCTTTGGAAGAGCGCTCACGAGCCGTAGCAGTTGTCTTTGGTGGTTTGAGTGTAGGAAGTGTTGCAGGGTTTCTTCTGGCTCCTCCCCTTATCCAAAATCTTGGCTGGGAATTTGTCTTTTACATATTTTCCCTTTTGGGGATAGGTTGGTATGTGGGTTTTCAGTTTCTTGAAGGACAAGCCTCATGGGTTGGATCCCGTTCAGACATGAAGACAGCGCAGCAAACTTCTCCAAAAGAGTTGGCTGACTCATTAAAGGATGTACCGTGGAAGGCATTTTTCCAAAGTGAAGCTGTTTGGGCAATGATATATGCTCATTTTTGTGGAAGCTGGGGTCATTATACTTGCCTAGCATGGCTTCCTACCTATTTTAGTGAGGAGCTGAACCTGAATTTGACCGAAGCGGCGTGGGTCTCTATCCTTCCTCCGTTGGCGTCAATATTTGTGACTAGTGTTGCATCCCAAATTGCTGATAACTTGATTTCTAGTGGAGTTCAAACCACTACGGTCCGAAAAATTTGCCAAACAATTGCCTTTTTGTCTCCTGCAGCTTGTATGACTCTTTCTTCTCTTGATCTAGGATTGCCCCATTGGGAAGTTGTGGGAATTCTCACTGGTGGTTTAGCCCTTTCGAGCTTTGCAATTTCAGGACTTTATTGTACCCATCAAGATATATCACCCGAATATGCAAGCATACTTTTGGGTATTACCAATACTGTTGGGGCGCTACCCGGAATAGTAGGTGTTGCCCTCACTGGCTATCTACTTGATTCAACTCATTCATGGAGTATGTCATTGTTCATTCCATCAATATTTTTCTACCTGACTGGTACAATTGTTTGGTTGGTGTTTGCCAGCAGTAAGCCTCAAACCTTTACAAAGAGAGATTGA

Protein Analysis

538

Amino Acids

58.18

Weight (kDa)

8.67

Isoelectric Point (pI)

45.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 117 - 490 2.1e-44 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 57, 201, 1130
AclWI GGATC 2 cut(s) 915, 928
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 6 cut(s) 140, 621, 836, 1117, 1242, 1332
AcuI CTGAAG 1 cut(s) 264
AcyI GRCGYC 1 cut(s) 1160
AfaI GTAC 3 cut(s) 990, 1387, 1558
AfeI AGCGCT 1 cut(s) 740
AfiI CCNNNNNNNGG 2 cut(s) 468, 864
AgsI TTSAA 4 cut(s) 139, 899, 1223, 1500
AhlI ACTAGT 1 cut(s) 1175
AjnI CCWGG 3 cut(s) 493, 601, 677
AluBI AGCT 9 cut(s) 30, 117, 135, 334, 1019, 1056, 1108, 1277, 1362
AluI AGCT 9 cut(s) 30, 117, 135, 334, 1019, 1056, 1108, 1277, 1362
Alw26I GTCTC 2 cut(s) 1144, 1272
AlwI GGATC 2 cut(s) 915, 928
AlwNI CAGNNNCTG 1 cut(s) 443
Aor51HI AGCGCT 1 cut(s) 740
AoxI GGCC 2 cut(s) 3, 299
ApeKI GCWGC 4 cut(s) 156, 659, 946, 1274
ApoI RAATTY 6 cut(s) 140, 621, 836, 1117, 1242, 1332
AspLEI GCGC 3 cut(s) 741, 946, 1453
AspS9I GGNCC 2 cut(s) 214, 1234
AsuC2I CCSGG 2 cut(s) 221, 1458
AsuHPI GGTGA 3 cut(s) 76, 394, 1395
AsuII TTCGAA 1 cut(s) 125
AvaII GGWCC 2 cut(s) 214, 1234
BaeI ACNNNNGTAYC 2 cut(s) 1548, 1581
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 920
BanII GRGCYC 1 cut(s) 198
BauI CACGAG 1 cut(s) 744
BbsI GAAGAC 1 cut(s) 944
BbvCI CCTCAGC 1 cut(s) 88
BbvI GCAGC 4 cut(s) 168, 646, 958, 1286
BccI CCATC 2 cut(s) 1398, 1540
BceAI ACGGC 2 cut(s) 87, 734
BcgI CGANNNNNNTGC 2 cut(s) 1227, 1261
BciT130I CCWGG 3 cut(s) 495, 603, 679
BcnI CCSGG 2 cut(s) 221, 1458
BcoDI GTCTC 2 cut(s) 1144, 1272
BcuI ACTAGT 1 cut(s) 1175
BfaI CTAG 4 cut(s) 1076, 1176, 1212, 1304
BfmI CTRYAG 2 cut(s) 567, 1272
BfoI RGCGCY 2 cut(s) 742, 1454
BisI GCNGC 5 cut(s) 157, 660, 947, 1131, 1275
BlpI GCTNAGC 1 cut(s) 113
BlsI GCNGC 5 cut(s) 158, 661, 948, 1132, 1276
Bme1390I CCNGG 5 cut(s) 221, 495, 603, 679, 1458
Bme18I GGWCC 2 cut(s) 214, 1234
BmgT120I GGNCC 2 cut(s) 214, 1234
BmiI GGNNCC 3 cut(s) 197, 807, 922
BmrFI CCNGG 5 cut(s) 221, 495, 603, 679, 1458
BmsI GCATC 2 cut(s) 424, 1193
BpiI GAAGAC 1 cut(s) 944
Bpu10I CCTNAGC 1 cut(s) 88
Bpu1102I GCTNAGC 1 cut(s) 113
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 2 cut(s) 409, 557
BpuMI CCSGG 2 cut(s) 221, 1458
BsaHI GRCGYC 1 cut(s) 1160
BsaI GGTCTC 1 cut(s) 1144
BsaJI CCNNGG 3 cut(s) 305, 494, 992
BsaXI ACNNNNNCTCC 7 cut(s) 30, 38, 68, 198, 228, 630, 660
Bsc4I CCNNNNNNNGG 2 cut(s) 468, 864
Bse1I ACTGG 4 cut(s) 302, 1345, 1486, 1558
Bse3DI GCAATG 1 cut(s) 1035
BseBI CCWGG 3 cut(s) 495, 603, 679
BseDI CCNNGG 3 cut(s) 305, 494, 992
BseGI GGATG 2 cut(s) 991, 1184
BseLI CCNNNNNNNGG 2 cut(s) 468, 864
BseMI GCAATG 1 cut(s) 1035
BseMII CTCAG 2 cut(s) 102, 104
BseNI ACTGG 4 cut(s) 302, 1345, 1486, 1558
BseRI GAGGAG 2 cut(s) 798, 1118
BseXI GCAGC 4 cut(s) 168, 646, 958, 1286
BseYI CCCAGC 3 cut(s) 69, 831, 1056
BshFI GGCC 2 cut(s) 5, 301
BsiSI CCGG 3 cut(s) 204, 220, 1458
BslI CCNNNNNNNGG 2 cut(s) 468, 864
BsmAI GTCTC 2 cut(s) 1144, 1272
BsnI GGCC 2 cut(s) 5, 301
Bso31I GGTCTC 1 cut(s) 1144
Bsp119I TTCGAA 1 cut(s) 125
Bsp1286I GDGCHC 1 cut(s) 198
Bsp143I GATC 2 cut(s) 920, 1300
Bsp1720I GCTNAGC 1 cut(s) 113
BspACI CCGC 3 cut(s) 57, 201, 1130
BspANI GGCC 2 cut(s) 5, 301
BspCNI CTCAG 2 cut(s) 101, 105
BspLI GGNNCC 3 cut(s) 197, 807, 922
BspMAI CTGCAG 1 cut(s) 1276
BspPI GGATC 2 cut(s) 915, 928
BspQI GCTCTTC 1 cut(s) 729
BspT104I TTCGAA 1 cut(s) 125
BspTNI GGTCTC 1 cut(s) 1144
BsrDI GCAATG 1 cut(s) 1035
BsrI ACTGG 4 cut(s) 302, 1345, 1486, 1558
BssECI CCNNGG 3 cut(s) 305, 494, 992
BssMI GATC 2 cut(s) 920, 1300
BssNI GRCGYC 1 cut(s) 1160
BssSI CACGAG 1 cut(s) 744
Bst2BI CACGAG 1 cut(s) 744
Bst2UI CCWGG 3 cut(s) 495, 603, 679
Bst4CI ACNGT 4 cut(s) 694, 993, 1234, 1444
Bst6I CTCTTC 2 cut(s) 82, 729
BstACI GRCGYC 1 cut(s) 1160
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 4 cut(s) 503, 507, 1417, 1582
BstDEI CTNAG 2 cut(s) 88, 113
BstDSI CCRYGG 2 cut(s) 305, 992
BstF5I GGATG 2 cut(s) 991, 1184
BstH2I RGCGCY 2 cut(s) 742, 1454
BstHHI GCGC 3 cut(s) 741, 946, 1453
BstKTI GATC 2 cut(s) 923, 1303
BstMAI GTCTC 2 cut(s) 1144, 1272
BstMBI GATC 2 cut(s) 920, 1300
BstMWI GCNNNNNNNGC 1 cut(s) 1025
BstNI CCWGG 3 cut(s) 495, 603, 679
BstSCI CCNGG 5 cut(s) 219, 493, 601, 677, 1456
BstSFI CTRYAG 2 cut(s) 567, 1272
BstV1I GCAGC 4 cut(s) 168, 646, 958, 1286
BstV2I GAAGAC 1 cut(s) 944
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 827
BstYI RGATCY 1 cut(s) 920
BsuRI GGCC 2 cut(s) 5, 301
BtgI CCRYGG 2 cut(s) 305, 992
BtsCI GGATG 2 cut(s) 991, 1184
BtsIMutI CAGTG 3 cut(s) 444, 1338, 1479
Cac8I GCNNGC 4 cut(s) 503, 507, 1417, 1582
CaiI CAGNNNCTG 1 cut(s) 443
CfoI GCGC 3 cut(s) 741, 946, 1453
Cfr13I GGNCC 2 cut(s) 214, 1234
CpoI CGGWCCG 1 cut(s) 1234
CseI GACGC 2 cut(s) 166, 1149
Csp6I GTAC 3 cut(s) 989, 1386, 1557
CspI CGGWCCG 1 cut(s) 1234
CviAII CATG 7 cut(s) 373, 409, 714, 912, 934, 1080, 1509
CviQI GTAC 3 cut(s) 989, 1386, 1557
DdeI CTNAG 2 cut(s) 88, 113
DpnI GATC 2 cut(s) 922, 1302
DpnII GATC 2 cut(s) 920, 1300
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 82, 729
EarI CTCTTC 2 cut(s) 82, 729
EciI GGCGGA 1 cut(s) 46
Eco24I GRGCYC 1 cut(s) 198
Eco31I GGTCTC 1 cut(s) 1144
Eco47I GGWCC 2 cut(s) 214, 1234
Eco47III AGCGCT 1 cut(s) 740
Eco57I CTGAAG 1 cut(s) 264
EcoRI GAATTC 1 cut(s) 1332
EcoRII CCWGG 3 cut(s) 493, 601, 677
EcoT38I GRGCYC 1 cut(s) 198
FaeI CATG 7 cut(s) 376, 412, 717, 915, 937, 1083, 1512
FalI AAGNNNNNCTT 4 cut(s) 119, 151, 371, 403
FatI CATG 7 cut(s) 372, 408, 713, 911, 933, 1079, 1508
FauI CCCGC 1 cut(s) 208
Fnu4HI GCNGC 5 cut(s) 157, 660, 947, 1131, 1275
FokI GGATG 2 cut(s) 998, 1171
FriOI GRGCYC 1 cut(s) 198
Fsp4HI GCNGC 5 cut(s) 157, 660, 947, 1131, 1275
FspBI CTAG 4 cut(s) 1076, 1176, 1212, 1304
GlaI GCGC 3 cut(s) 740, 945, 1452
GluI GCNGC 5 cut(s) 157, 660, 947, 1131, 1275
GsaI CCCAGC 3 cut(s) 73, 835, 1060
HaeII RGCGCY 2 cut(s) 742, 1454
HaeIII GGCC 2 cut(s) 5, 301
HapII CCGG 3 cut(s) 204, 220, 1458
HgaI GACGC 2 cut(s) 166, 1149
HhaI GCGC 3 cut(s) 741, 946, 1453
Hin1I GRCGYC 1 cut(s) 1160
Hin1II CATG 7 cut(s) 376, 412, 717, 915, 937, 1083, 1512
Hin6I GCGC 3 cut(s) 739, 944, 1451
HinP1I GCGC 3 cut(s) 739, 944, 1451
HinfI GANTC 7 cut(s) 147, 239, 251, 484, 974, 1285, 1496
HpaII CCGG 3 cut(s) 204, 220, 1458
HphI GGTGA 3 cut(s) 76, 394, 1395
Hpy166II GTNNAC 2 cut(s) 217, 385
Hpy188I TCNGA 6 cut(s) 20, 146, 238, 244, 931, 1238
Hpy188III TCNNGA 9 cut(s) 181, 260, 536, 618, 744, 896, 1298, 1374, 1394
Hpy8I GTNNAC 2 cut(s) 217, 385
Hpy99I CGWCG 1 cut(s) 108
HpyAV CCTTC 8 cut(s) 132, 163, 239, 472, 635, 893, 991, 1157
HpyCH4III ACNGT 4 cut(s) 694, 993, 1234, 1444
HpyCH4V TGCA 8 cut(s) 156, 662, 717, 791, 1184, 1274, 1367, 1415
HpyF10VI GCNNNNNNNGC 1 cut(s) 1025
HpyF3I CTNAG 2 cut(s) 88, 113
Hsp92I GRCGYC 1 cut(s) 1160
Hsp92II CATG 7 cut(s) 376, 412, 717, 915, 937, 1083, 1512
HspAI GCGC 3 cut(s) 739, 944, 1451
Kzo9I GATC 2 cut(s) 920, 1300
LguI GCTCTTC 1 cut(s) 729
LmnI GCTCC 4 cut(s) 35, 201, 811, 1105
Lsp1109I GCAGC 4 cut(s) 168, 646, 958, 1286
LweI GCATC 2 cut(s) 424, 1193
MaeI CTAG 4 cut(s) 1076, 1176, 1212, 1304
MaeIII GTNAC 2 cut(s) 368, 1171
MalI GATC 2 cut(s) 922, 1302
MboI GATC 2 cut(s) 920, 1300
MboII GAAGA 6 cut(s) 69, 322, 746, 791, 949, 1284
MfeI CAATTG 3 cut(s) 488, 1254, 1560
MflI RGATCY 1 cut(s) 920
MhlI GDGCHC 1 cut(s) 198
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 5 cut(s) 156, 260, 493, 968, 1279
MmeI TCCRAC 2 cut(s) 407, 898
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 4 cut(s) 162, 551, 696, 980
MslI CAYNNNNRTG 1 cut(s) 1047
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 3 cut(s) 204, 220, 1458
MspR9I CCNGG 5 cut(s) 221, 495, 603, 679, 1458
MunI CAATTG 3 cut(s) 488, 1254, 1560
MvaI CCWGG 3 cut(s) 495, 603, 679
MwoI GCNNNNNNNGC 1 cut(s) 1025
NciI CCSGG 2 cut(s) 221, 1458
NdeII GATC 2 cut(s) 920, 1300
NlaIII CATG 7 cut(s) 376, 412, 717, 915, 937, 1083, 1512
NlaIV GGNNCC 3 cut(s) 197, 807, 922
NmuCI GTSAC 1 cut(s) 1171
NspV TTCGAA 1 cut(s) 125
PciSI GCTCTTC 1 cut(s) 729
PfeI GAWTC 2 cut(s) 239, 1496
PkrI GCNGC 5 cut(s) 158, 661, 948, 1132, 1276
PleI GAGTC 5 cut(s) 155, 259, 492, 968, 1279
PpsI GAGTC 5 cut(s) 155, 259, 492, 968, 1279
Psp6I CCWGG 3 cut(s) 493, 601, 677
PspFI CCCAGC 3 cut(s) 69, 831, 1056
PspGI CCWGG 3 cut(s) 493, 601, 677
PspN4I GGNNCC 3 cut(s) 197, 807, 922
PspPI GGNCC 2 cut(s) 214, 1234
PstI CTGCAG 1 cut(s) 1276
PstNI CAGNNNCTG 1 cut(s) 443
PsuI RGATCY 1 cut(s) 920
RsaI GTAC 3 cut(s) 990, 1387, 1558
RsaNI GTAC 3 cut(s) 989, 1386, 1557
RseI CAYNNNNRTG 1 cut(s) 1047
Rsr2I CGGWCCG 1 cut(s) 1234
RsrII CGGWCCG 1 cut(s) 1234
SapI GCTCTTC 1 cut(s) 729
SaqAI TTAA 4 cut(s) 162, 551, 696, 980
SatI GCNGC 5 cut(s) 157, 660, 947, 1131, 1275
Sau3AI GATC 2 cut(s) 920, 1300
Sau96I GGNCC 2 cut(s) 214, 1234
SchI GAGTC 5 cut(s) 156, 260, 493, 968, 1279
ScrFI CCNGG 5 cut(s) 221, 495, 603, 679, 1458
SduI GDGCHC 1 cut(s) 198
SfaNI GCATC 2 cut(s) 424, 1193
SfcI CTRYAG 2 cut(s) 567, 1272
SfuI TTCGAA 1 cut(s) 125
SinI GGWCC 2 cut(s) 214, 1234
SmiMI CAYNNNNRTG 1 cut(s) 1047
SmlI CTYRAG 2 cut(s) 388, 536
SmoI CTYRAG 2 cut(s) 388, 536
SpeI ACTAGT 1 cut(s) 1175
SsiI CCGC 3 cut(s) 57, 201, 1130
SspI AATATT 2 cut(s) 1167, 1539
SspMI CTAG 4 cut(s) 1076, 1176, 1212, 1304
StyD4I CCNGG 5 cut(s) 219, 493, 601, 677, 1456
TaaI ACNGT 4 cut(s) 694, 993, 1234, 1444
TaqI TCGA 2 cut(s) 125, 1358
TaqII GACCGA 1 cut(s) 1139
TauI GCSGC 1 cut(s) 1133
TfiI GAWTC 2 cut(s) 239, 1496
Tru1I TTAA 4 cut(s) 162, 551, 696, 980
Tru9I TTAA 4 cut(s) 162, 551, 696, 980
TscAI CASTG 3 cut(s) 444, 1345, 1486
TseFI GTSAC 1 cut(s) 1171
TseI GCWGC 4 cut(s) 156, 659, 946, 1274
Tsp45I GTSAC 1 cut(s) 1171
TspDTI ATGAA 6 cut(s) 339, 423, 702, 950, 1497, 1516
TspGWI ACGGA 2 cut(s) 294, 1143
TspRI CASTG 3 cut(s) 444, 1345, 1486
VpaK11BI GGWCC 2 cut(s) 214, 1234
XapI RAATTY 6 cut(s) 140, 621, 836, 1117, 1242, 1332
XspI CTAG 4 cut(s) 1076, 1176, 1212, 1304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.