MD15G1162900.v1.1

anion transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0009581
Physical Location & Seq
Reverse (-)
12283808 .. 12289489
5682 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1162900.v1.1.491

Sequence Viewer

Length: 1596 bp
ATGGCGAAGCTCACACTCAGAGCCGAGAGCTCCTCGTGCCTATTTTCACACAGCACGGGATCGCAGCCATCGTCGTTCGTGTCCACGCTCGGACGGCGTAGTTTGATTCGGTTCCCTCCGAAGCATAATTTGGAGTTCAGAGTGTTTTGTAGTATTAAGGAGAAGGAAAGCGTCAGAGAAACTAAGAGGGTCAATGGGTTACCGGTGGATAAGCTCCAACGGGCGGGTTCGGGGTCTGGGTCGGATTCTGAGGGTGAATCGGGTCTGAACAGCGGGTCTGGGGAAGTGGGTTTTGATTGGGATTGGCCTCCGTGGAAGAGCGTGCCTCGGAGGTACAAGCTCATTGGGACTACCTCGCTTGCGTTTGTTATCTGCAATATGGATAAGGTGAACTTGAGTATTGCTATAATTCCAATGTCCCACCAGTTTGGATGGAGTTCATCCGAGGCGGGATTGGTTCAATCCTCGTTCTTTTGGGGGTATGCATTGAGTCAGTTGCCTGGTGGCTGGCTTGCCAAGATATTTGGTGGGAGAAAAGTTCTTGAGTTTGGAGTATTAACTTGGTCTTTGGCTACAGCACTTGTACCTCTTGTTGCTGGATTTACACCTGGTTTAGTTTTGTCAAGAATTTTGGAATTGGAGAAGGCGTTTCCCCATCCGCTGCAACGGACCTTATTGCCAGGCATGACAATACCATTGGAAGAGCGGTCACGGGCTGTAGCATTTGTCTTCGGTGGTCTGAGTGTAGGAAGTGTTGCAGGGTTTCTTCTGGCTCCTCCCCTTATCCAAAATCTTGGCTGGGAATCTGTTTTTTACATATTTTCCTTTTTGGGGATTGCTTGGTATCTGGGATTTCAGTTTCTTGAAGGACAAGCCTCATGGGTTGGTGAATCTATTCCACGGTCCCAATCAACAGATGTGAAGAAAACATGGAGTACCGAAGAATTAGGTGACTCTTTGAAGGGTGTACCATGGAAAGCATTTTTTCAAAGTCAAGCTGTATGGGCAATGATATATGCTCATTTTTGTGGAAGCTGGGGTCATTATACTTGCCTAGCATGGCTTCCCACCTATTTTAGTGAGGAGCTGAACCTGAATTTGACAGAAGCTGCATGGGTCTCTATCCTTCCTCCATTGGCTTCAATCTTTGTGACTAGCATTGCATCACAATTTGCTGATAGCTTGATTTCTAGTGGAGTTCAAACCACTACAGTCCGAAAAATTTGCCAAACAATTGCCTTTTTATCTCCTGCAGCTTGCATGACTCTTTCCTCTGTTGATCTAGGATTGCCCCATTGGGAAGTTGTTGGAATTCTCACTGGTGGTTTAGCCCTCTCAAGCTTTGCGTTGTCAGGATTGTATTGTACCCATCAAGACATGTCACCCGAATACGCAAGCATACTTTTGGGTATTACCAACACTGTTGGGGCAGTACCTGGAATAATAGGTGTTGCCCTCACCGGCATTCTTCTTGATTCAACTCATTCATGGAGTATATCATTGTTCATTCCATCAATATTTTTCTACCTGACTGGTACAGTCGTTTGGTTGGCGTTTGCCAGCAGTAAGCCTCAATCCTTTTCGAAGAGAGACTGA

Protein Analysis

532

Amino Acids

57.75

Weight (kDa)

7.61

Isoelectric Point (pI)

47.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 119 - 483 1.9e-41 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 706
AciI CCGC 5 cut(s) 224, 273, 449, 659, 706
AclWI GGATC 1 cut(s) 67
AcsI RAATTY 4 cut(s) 627, 1096, 1221, 1311
AfaI GTAC 7 cut(s) 335, 585, 937, 969, 1366, 1434, 1537
AfiI CCNNNNNNNGG 2 cut(s) 223, 831
AflIII ACRYGT 1 cut(s) 1377
AgeI ACCGGT 1 cut(s) 202
AgsI TTSAA 7 cut(s) 461, 866, 961, 989, 1143, 1202, 1479
AjnI CCWGG 4 cut(s) 499, 607, 679, 1435
AjuI GAANNNNNNNTTGG 2 cut(s) 113, 145
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 1123, 1584
AlwI GGATC 1 cut(s) 67
AlwNI CAGNNNCTG 2 cut(s) 1109, 1436
AoxI GGCC 1 cut(s) 305
ApeKI GCWGC 4 cut(s) 64, 661, 1109, 1253
ApoI RAATTY 4 cut(s) 627, 1096, 1221, 1311
AsiGI ACCGGT 1 cut(s) 202
Asp700I GAANNNNTTC 1 cut(s) 894
AspS9I GGNCC 2 cut(s) 669, 903
AsuHPI GGTGA 6 cut(s) 266, 400, 899, 962, 1374, 1450
AsuII TTCGAA 1 cut(s) 1583
AvaII GGWCC 2 cut(s) 669, 903
BaeI ACNNNNGTAYC 2 cut(s) 1527, 1560
BanII GRGCYC 1 cut(s) 32
BauI CACGAG 1 cut(s) 34
BbsI GAAGAC 1 cut(s) 721
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 4 cut(s) 76, 648, 1096, 1265
BccI CCATC 5 cut(s) 76, 426, 663, 1377, 1519
BceAI ACGGC 1 cut(s) 110
BcgI CGANNNNNNTGC 2 cut(s) 1206, 1240
BciT130I CCWGG 4 cut(s) 501, 609, 681, 1437
BcoDI GTCTC 2 cut(s) 1123, 1584
BfaI CTAG 4 cut(s) 1055, 1155, 1191, 1283
BfmI CTRYAG 4 cut(s) 573, 717, 1209, 1251
BisI GCNGC 4 cut(s) 65, 662, 1110, 1254
BlsI GCNGC 4 cut(s) 66, 663, 1111, 1255
Bme1390I CCNGG 4 cut(s) 501, 609, 681, 1437
Bme18I GGWCC 2 cut(s) 669, 903
BmgT120I GGNCC 2 cut(s) 669, 903
BmiI GGNNCC 3 cut(s) 113, 774, 905
BmrFI CCNGG 4 cut(s) 501, 609, 681, 1437
BmsI GCATC 1 cut(s) 1172
BpiI GAAGAC 1 cut(s) 721
BplI GAGNNNNNCTC 4 cut(s) 17, 49, 310, 342
Bpu14I TTCGAA 1 cut(s) 1583
BpuEI CTTGAG 3 cut(s) 415, 563, 1321
BsaI GGTCTC 1 cut(s) 1123
BsaJI CCNNGG 5 cut(s) 311, 326, 444, 899, 971
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 831
Bse118I RCCGGY 2 cut(s) 202, 1460
Bse1I ACTGG 3 cut(s) 424, 1324, 1537
Bse3DI GCAATG 2 cut(s) 1014, 1158
BseBI CCWGG 4 cut(s) 501, 609, 681, 1437
BseDI CCNNGG 5 cut(s) 311, 326, 444, 899, 971
BseGI GGATG 3 cut(s) 437, 440, 655
BseLI CCNNNNNNNGG 2 cut(s) 223, 831
BseMI GCAATG 2 cut(s) 1014, 1158
BseMII CTCAG 3 cut(s) 31, 240, 731
BseNI ACTGG 3 cut(s) 424, 1324, 1537
BseRI GAGGAG 3 cut(s) 22, 765, 1097
BseXI GCAGC 4 cut(s) 76, 648, 1096, 1265
BseYI CCCAGC 2 cut(s) 798, 1035
BshFI GGCC 1 cut(s) 307
BshTI ACCGGT 1 cut(s) 202
BsiHKAI GWGCWC 1 cut(s) 32
BsiSI CCGG 2 cut(s) 203, 1461
BslFI GGGAC 3 cut(s) 361, 403, 889
BslI CCNNNNNNNGG 2 cut(s) 223, 831
BsmAI GTCTC 2 cut(s) 1123, 1584
BsmFI GGGAC 3 cut(s) 361, 403, 889
BsmI GAATGC 1 cut(s) 1464
BsnI GGCC 1 cut(s) 307
Bso31I GGTCTC 1 cut(s) 1123
Bsp119I TTCGAA 1 cut(s) 1583
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 2 cut(s) 59, 1279
Bsp19I CCATGG 1 cut(s) 971
BspACI CCGC 5 cut(s) 224, 273, 449, 659, 706
BspANI GGCC 1 cut(s) 307
BspCNI CTCAG 3 cut(s) 30, 241, 732
BspLI GGNNCC 3 cut(s) 113, 774, 905
BspMAI CTGCAG 1 cut(s) 1255
BspPI GGATC 1 cut(s) 67
BspQI GCTCTTC 2 cut(s) 311, 696
BspT104I TTCGAA 1 cut(s) 1583
BspTNI GGTCTC 1 cut(s) 1123
BsrBI CCGCTC 1 cut(s) 706
BsrDI GCAATG 2 cut(s) 1014, 1158
BsrFI RCCGGY 2 cut(s) 202, 1460
BsrI ACTGG 3 cut(s) 424, 1324, 1537
BssAI RCCGGY 2 cut(s) 202, 1460
BssECI CCNNGG 5 cut(s) 311, 326, 444, 899, 971
BssMI GATC 2 cut(s) 59, 1279
BssSI CACGAG 1 cut(s) 34
BssT1I CCWWGG 1 cut(s) 971
Bst2BI CACGAG 1 cut(s) 34
Bst2UI CCWGG 4 cut(s) 501, 609, 681, 1437
Bst4CI ACNGT 4 cut(s) 903, 1213, 1423, 1540
Bst6I CTCTTC 3 cut(s) 311, 696, 1580
BstBI TTCGAA 1 cut(s) 1583
BstC8I GCNNGC 7 cut(s) 323, 360, 509, 513, 1258, 1396, 1561
BstDEI CTNAG 4 cut(s) 17, 183, 249, 740
BstDSI CCRYGG 3 cut(s) 311, 899, 971
BstEII GGTNACC 1 cut(s) 198
BstF5I GGATG 3 cut(s) 437, 440, 655
BstKTI GATC 2 cut(s) 62, 1282
BstMAI GTCTC 2 cut(s) 1123, 1584
BstMBI GATC 2 cut(s) 59, 1279
BstMWI GCNNNNNNNGC 3 cut(s) 36, 94, 1004
BstNI CCWGG 4 cut(s) 501, 609, 681, 1437
BstNSI RCATGY 1 cut(s) 1381
BstPI GGTNACC 1 cut(s) 198
BstSCI CCNGG 4 cut(s) 499, 607, 679, 1435
BstSFI CTRYAG 4 cut(s) 573, 717, 1209, 1251
BstV1I GCAGC 4 cut(s) 76, 648, 1096, 1265
BstV2I GAAGAC 1 cut(s) 721
BstXI CCANNNNNNTGG 2 cut(s) 428, 794
BsuRI GGCC 1 cut(s) 307
BtgI CCRYGG 3 cut(s) 311, 899, 971
BtsCI GGATG 3 cut(s) 437, 440, 655
BtsIMutI CAGTG 2 cut(s) 1317, 1419
Cac8I GCNNGC 7 cut(s) 323, 360, 509, 513, 1258, 1396, 1561
CaiI CAGNNNCTG 2 cut(s) 1109, 1436
Cfr10I RCCGGY 2 cut(s) 202, 1460
Cfr13I GGNCC 2 cut(s) 669, 903
CseI GACGC 1 cut(s) 160
CsiI ACCWGGT 1 cut(s) 607
Csp6I GTAC 7 cut(s) 334, 584, 936, 968, 1365, 1433, 1536
CspAI ACCGGT 1 cut(s) 202
CviAII CATG 9 cut(s) 685, 879, 930, 972, 1059, 1113, 1261, 1378, 1488
CviQI GTAC 7 cut(s) 334, 584, 936, 968, 1365, 1433, 1536
DdeI CTNAG 4 cut(s) 17, 183, 249, 740
DpnI GATC 2 cut(s) 61, 1281
DpnII GATC 2 cut(s) 59, 1279
Eam1104I CTCTTC 3 cut(s) 311, 696, 1580
EarI CTCTTC 3 cut(s) 311, 696, 1580
Ecl136II GAGCTC 1 cut(s) 30
Eco130I CCWWGG 1 cut(s) 971
Eco24I GRGCYC 1 cut(s) 32
Eco31I GGTCTC 1 cut(s) 1123
Eco47I GGWCC 2 cut(s) 669, 903
Eco53kI GAGCTC 1 cut(s) 30
Eco91I GGTNACC 1 cut(s) 198
EcoICRI GAGCTC 1 cut(s) 30
EcoO65I GGTNACC 1 cut(s) 198
EcoRI GAATTC 1 cut(s) 1311
EcoRII CCWGG 4 cut(s) 499, 607, 679, 1435
EcoT14I CCWWGG 1 cut(s) 971
EcoT22I ATGCAT 1 cut(s) 487
EcoT38I GRGCYC 1 cut(s) 32
ErhI CCWWGG 1 cut(s) 971
FaeI CATG 9 cut(s) 688, 882, 933, 975, 1062, 1116, 1264, 1381, 1491
FalI AAGNNNNNCTT 2 cut(s) 377, 409
FaqI GGGAC 3 cut(s) 361, 403, 889
FatI CATG 9 cut(s) 684, 878, 929, 971, 1058, 1112, 1260, 1377, 1487
FauI CCCGC 3 cut(s) 217, 266, 442
Fnu4HI GCNGC 4 cut(s) 65, 662, 1110, 1254
FokI GGATG 3 cut(s) 427, 444, 642
FriOI GRGCYC 1 cut(s) 32
Fsp4HI GCNGC 4 cut(s) 65, 662, 1110, 1254
FspBI CTAG 4 cut(s) 1055, 1155, 1191, 1283
GluI GCNGC 4 cut(s) 65, 662, 1110, 1254
GsaI CCCAGC 2 cut(s) 802, 1039
HaeIII GGCC 1 cut(s) 307
HapII CCGG 2 cut(s) 203, 1461
HgaI GACGC 1 cut(s) 160
Hin1II CATG 9 cut(s) 688, 882, 933, 975, 1062, 1116, 1264, 1381, 1491
HindIII AAGCTT 1 cut(s) 1339
HinfI GANTC 9 cut(s) 106, 245, 257, 490, 803, 890, 953, 1264, 1475
HpaII CCGG 2 cut(s) 203, 1461
HphI GGTGA 6 cut(s) 266, 400, 899, 962, 1374, 1450
Hpy166II GTNNAC 3 cut(s) 84, 391, 968
Hpy188III TCNNGA 6 cut(s) 542, 624, 863, 1353, 1373, 1472
Hpy8I GTNNAC 3 cut(s) 84, 391, 968
Hpy99I CGWCG 1 cut(s) 76
HpyAV CCTTC 5 cut(s) 157, 637, 860, 955, 1136
HpyCH4III ACNGT 4 cut(s) 903, 1213, 1423, 1540
HpyCH4V TGCA 8 cut(s) 375, 485, 664, 758, 1112, 1163, 1253, 1260
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 94, 1004
HpyF3I CTNAG 4 cut(s) 17, 183, 249, 740
Hsp92II CATG 9 cut(s) 688, 882, 933, 975, 1062, 1116, 1264, 1381, 1491
Kzo9I GATC 2 cut(s) 59, 1279
LguI GCTCTTC 2 cut(s) 311, 696
LmnI GCTCC 4 cut(s) 35, 219, 778, 1084
Lsp1109I GCAGC 4 cut(s) 76, 648, 1096, 1265
LweI GCATC 1 cut(s) 1172
MabI ACCWGGT 1 cut(s) 607
MaeI CTAG 4 cut(s) 1055, 1155, 1191, 1283
MaeIII GTNAC 5 cut(s) 198, 708, 950, 1150, 1380
MalI GATC 2 cut(s) 61, 1281
MbiI CCGCTC 1 cut(s) 706
MboI GATC 2 cut(s) 59, 1279
MboII GAAGA 7 cut(s) 328, 713, 721, 758, 934, 953, 1460
MfeI CAATTG 1 cut(s) 1233
MhlI GDGCHC 1 cut(s) 32
MlyI GAGTC 3 cut(s) 499, 947, 1258
MmeI TCCRAC 3 cut(s) 222, 241, 1288
Mph1103I ATGCAT 1 cut(s) 487
MroXI GAANNNNTTC 1 cut(s) 894
MseI TTAA 2 cut(s) 156, 557
MslI CAYNNNNRTG 1 cut(s) 1026
MspA1I CMGCKG 2 cut(s) 273, 661
MspI CCGG 2 cut(s) 203, 1461
MspR9I CCNGG 4 cut(s) 501, 609, 681, 1437
MunI CAATTG 1 cut(s) 1233
Mva1269I GAATGC 1 cut(s) 1464
MvaI CCWGG 4 cut(s) 501, 609, 681, 1437
MwoI GCNNNNNNNGC 3 cut(s) 36, 94, 1004
NcoI CCATGG 1 cut(s) 971
NdeII GATC 2 cut(s) 59, 1279
NlaIII CATG 9 cut(s) 688, 882, 933, 975, 1062, 1116, 1264, 1381, 1491
NlaIV GGNNCC 3 cut(s) 113, 774, 905
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 4 cut(s) 708, 950, 1150, 1380
NsiI ATGCAT 1 cut(s) 487
NspI RCATGY 1 cut(s) 1381
NspV TTCGAA 1 cut(s) 1583
PciI ACATGT 1 cut(s) 1377
PciSI GCTCTTC 2 cut(s) 311, 696
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PctI GAATGC 1 cut(s) 1464
PdmI GAANNNNTTC 1 cut(s) 894
PfeI GAWTC 6 cut(s) 106, 245, 257, 803, 890, 1475
PinAI ACCGGT 1 cut(s) 202
PkrI GCNGC 4 cut(s) 66, 663, 1111, 1255
PleI GAGTC 3 cut(s) 498, 947, 1258
PpsI GAGTC 3 cut(s) 498, 947, 1258
PscI ACATGT 1 cut(s) 1377
Psp124BI GAGCTC 1 cut(s) 32
Psp6I CCWGG 4 cut(s) 499, 607, 679, 1435
PspEI GGTNACC 1 cut(s) 198
PspFI CCCAGC 2 cut(s) 798, 1035
PspGI CCWGG 4 cut(s) 499, 607, 679, 1435
PspN4I GGNNCC 3 cut(s) 113, 774, 905
PspPI GGNCC 2 cut(s) 669, 903
PstI CTGCAG 1 cut(s) 1255
PstNI CAGNNNCTG 2 cut(s) 1109, 1436
RsaI GTAC 7 cut(s) 335, 585, 937, 969, 1366, 1434, 1537
RsaNI GTAC 7 cut(s) 334, 584, 936, 968, 1365, 1433, 1536
RseI CAYNNNNRTG 1 cut(s) 1026
SacI GAGCTC 1 cut(s) 32
SapI GCTCTTC 2 cut(s) 311, 696
SaqAI TTAA 2 cut(s) 156, 557
SatI GCNGC 4 cut(s) 65, 662, 1110, 1254
Sau3AI GATC 2 cut(s) 59, 1279
Sau96I GGNCC 2 cut(s) 669, 903
SchI GAGTC 3 cut(s) 499, 947, 1258
ScrFI CCNGG 4 cut(s) 501, 609, 681, 1437
SduI GDGCHC 1 cut(s) 32
SexAI ACCWGGT 1 cut(s) 607
SfaNI GCATC 1 cut(s) 1172
SfcI CTRYAG 4 cut(s) 573, 717, 1209, 1251
SfuI TTCGAA 1 cut(s) 1583
SinI GGWCC 2 cut(s) 669, 903
SmiMI CAYNNNNRTG 1 cut(s) 1026
SmlI CTYRAG 3 cut(s) 394, 542, 1336
SmoI CTYRAG 3 cut(s) 394, 542, 1336
SsiI CCGC 5 cut(s) 224, 273, 449, 659, 706
SspI AATATT 1 cut(s) 1518
SspMI CTAG 4 cut(s) 1055, 1155, 1191, 1283
SstI GAGCTC 1 cut(s) 32
StyD4I CCNGG 4 cut(s) 499, 607, 679, 1435
StyI CCWWGG 1 cut(s) 971
TaaI ACNGT 4 cut(s) 903, 1213, 1423, 1540
TaqI TCGA 1 cut(s) 1583
TfiI GAWTC 6 cut(s) 106, 245, 257, 803, 890, 1475
Tru1I TTAA 2 cut(s) 156, 557
Tru9I TTAA 2 cut(s) 156, 557
TscAI CASTG 2 cut(s) 1324, 1426
TseFI GTSAC 4 cut(s) 708, 950, 1150, 1380
TseI GCWGC 4 cut(s) 64, 661, 1109, 1253
Tsp45I GTSAC 4 cut(s) 708, 950, 1150, 1380
TspDTI ATGAA 3 cut(s) 429, 1476, 1495
TspGWI ACGGA 2 cut(s) 300, 682
TspRI CASTG 2 cut(s) 1324, 1426
VpaK11BI GGWCC 2 cut(s) 669, 903
XapI RAATTY 4 cut(s) 627, 1096, 1221, 1311
XceI RCATGY 1 cut(s) 1381
XmnI GAANNNNTTC 1 cut(s) 894
XspI CTAG 4 cut(s) 1055, 1155, 1191, 1283
Zsp2I ATGCAT 1 cut(s) 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.