MD02G1019200.v1.1

anion transporter 6

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0009581
Physical Location & Seq
Reverse (-)
1389596 .. 1394132
4537 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1019200.v1.1.491

Sequence Viewer

Length: 1512 bp
ATGGCGAAGCTCACGCTCAGAGCCGAGTGCTCCTCCTGCTTATTTTCACACAGCACAAGCTCACAATCTCCCCTCTCCTCACCCAGACGACGTCGTTTGCTTCGGTTTCCTCCGAAGAACAACTTGGAGTTGAGAGTGATTTGTAGCATTCAGGAGAGGGAAAACGTCAGGGAAACCAAGAGGGTCAATGGGCTACCGGTGGATAAGGTCCAACGGGCGGGTTCGGGTTCTGGGACGGATTCTGAGGGGGAATTGAGTCAGGAGAGCGGGTCTGGGGAAGTGGGTTTTGATTGGGATTGGCCGCCGTGGAAGAACATACCTCAGAGGTACAAGCTCATTGGGACTACTTCGCTTGCCTTTGTCATCTGCAACATGGATAAGGTGAACTTGAGTATTGCTATAATTCCAATGTCTCACCAGTTTGGATGGAGTTCATCCGAGGCGGGATTGGTTCAATCGTCGTTCTTTTGGGGGTATGCATTGAGTCAGTTGCCCGGAGGTTGGCTTGCCAAGATATTTAGTGGGAGAAAAGTTCTTCAGTTTGGAGTGTTAACTTGGTCTTTGGCTACGGGACTTGTCCCTCTTGTTGCTGGATTTACACCTGGTTTAGTTTTGTCCAGAATTTTGGTAGGTATTGGAGAAGGCGTTTCCCCATCCGCCGCAACTGACCTTATTGCCAGGACAATACCTTTGGAAGAGCGTTCACGGGCTGTAGCATTTGTCTTTGGTGGTCTGAGTGTAGGAAGTGTGGCAGGGTTTCTTCTGGCTCCTCCCCTTATCCAAAATCTGGGGTGGGAATCTGTATTTTACATATTTTCCTCGTTGGGGATTGCTTGGTATCTGGGGTTTCAGTTTCTCGAAGGACAAGCCTCATGGGTTGGTGAATCAATTCCACGGTCCCAATCAACAGATGTGAAGAAAACATGGAGTACCGAAGAATTGGGTGACTCGTTGAAGGATGTACCATGGAAAGCATTTTTCCAAAGTCCAGCTGTATGGGCAATGATCTATGCTCATTTTTGTGGAAGCTGGGGTCATTATACTTGCCTAGCGTGGCTTCCCACCTATTTTAGTGAAGAGCTGAACCTGAATTTGACAGAAGCTGCATGGGTCTCTATCCTTCCTCCATTGGCTTCGATCTTTGTGACCAGCATTGCATCACAATTTGCTGATAGCTTGATTTCTACTGGAGTTCAAACCACTACGGTCCGAAAAGTTTGCCAAACAATTGCCTTTTTATCTCCTGCAGCTTGCATGACTCTTTCATCTCTTGATCTAGGATTGCCGCATTGGGAAGTTGTTGGGATTCTCACTGGTGGTTTAGCCCTCTCGAGCTTTGCCTTGTCAGGATTGTATTGTACCCATCAAGACATGTCACCCGAATATGCGAGCATACTTTTGGTATGTAAACTTTACACACGAATTTTTATTAGTGTGCTGGAAGCCTGGAAGATGCTATGGAGCGTCCTTTTTTTGTTTGTATCCTATCTCTTCCCTTTGCTTGATTATTAA

Protein Analysis

504

Amino Acids

55.25

Weight (kDa)

6.61

Isoelectric Point (pI)

52.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_1 PF07690 117 - 455 1.4e-44 Major Facilitator Superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 94
AccB7I CCANNNNNTGG 1 cut(s) 787
AccBSI CCGCTC 1 cut(s) 267
AciI CCGC 7 cut(s) 218, 267, 302, 443, 657, 660, 1286
AcoI YGGCCR 1 cut(s) 299
AcsI RAATTY 3 cut(s) 621, 1090, 1422
AcuI CTGAAG 1 cut(s) 521
AcyI GRCGYC 1 cut(s) 91
AfaI GTAC 4 cut(s) 329, 931, 963, 1360
AfiI CCNNNNNNNGG 4 cut(s) 217, 501, 787, 825
AflIII ACRYGT 1 cut(s) 1371
AgeI ACCGGT 1 cut(s) 196
AgsI TTSAA 3 cut(s) 455, 955, 1196
AjnI CCWGG 3 cut(s) 601, 677, 1445
AjuI GAANNNNNNNTTGG 2 cut(s) 107, 139
Alw21I GWGCWC 1 cut(s) 32
Alw26I GTCTC 2 cut(s) 417, 1117
AlwNI CAGNNNCTG 1 cut(s) 1103
Ama87I CYCGRG 1 cut(s) 1330
AoxI GGCC 1 cut(s) 299
ApeKI GCWGC 2 cut(s) 1103, 1247
ApoI RAATTY 3 cut(s) 621, 1090, 1422
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
AsiGI ACCGGT 1 cut(s) 196
Asp700I GAANNNNTTC 1 cut(s) 888
AspS9I GGNCC 3 cut(s) 208, 897, 1207
AsuC2I CCSGG 1 cut(s) 495
AsuHPI GGTGA 6 cut(s) 72, 394, 407, 893, 956, 1368
AvaI CYCGRG 1 cut(s) 1330
AvaII GGWCC 3 cut(s) 208, 897, 1207
Bbv12I GWGCWC 1 cut(s) 32
BbvI GCAGC 2 cut(s) 1090, 1259
BccI CCATC 3 cut(s) 420, 661, 1371
BceAI ACGGC 1 cut(s) 289
BcgI CGANNNNNNTGC 2 cut(s) 1200, 1234
BciT130I CCWGG 3 cut(s) 603, 679, 1447
BciVI GTATCC 1 cut(s) 1492
BcnI CCSGG 1 cut(s) 495
BcoDI GTCTC 2 cut(s) 417, 1117
BfaI CTAG 2 cut(s) 1049, 1277
BfmI CTRYAG 2 cut(s) 711, 1245
BfuI GTATCC 1 cut(s) 1492
BisI GCNGC 5 cut(s) 302, 660, 1104, 1248, 1286
BlsI GCNGC 5 cut(s) 303, 661, 1105, 1249, 1287
Bme1390I CCNGG 4 cut(s) 495, 603, 679, 1447
Bme18I GGWCC 3 cut(s) 208, 897, 1207
BmeT110I CYCGRG 1 cut(s) 1330
BmgT120I GGNCC 3 cut(s) 208, 897, 1207
BmiI GGNNCC 2 cut(s) 768, 899
BmrFI CCNGG 4 cut(s) 495, 603, 679, 1447
BmsI GCATC 2 cut(s) 1166, 1443
BplI GAGNNNNNCTC 2 cut(s) 17, 49
BpmI CTGGAG 1 cut(s) 1209
BpuEI CTTGAG 1 cut(s) 409
BpuMI CCSGG 1 cut(s) 495
BsaHI GRCGYC 1 cut(s) 91
BsaI GGTCTC 1 cut(s) 1117
BsaJI CCNNGG 4 cut(s) 305, 438, 893, 965
BsaWI WCCGGW 1 cut(s) 196
BsaXI ACNNNNNCTCC 4 cut(s) 254, 284, 630, 660
Bsc4I CCNNNNNNNGG 4 cut(s) 217, 501, 787, 825
Bse118I RCCGGY 1 cut(s) 196
Bse1I ACTGG 3 cut(s) 418, 1192, 1318
Bse3DI GCAATG 2 cut(s) 1008, 1152
BseBI CCWGG 3 cut(s) 603, 679, 1447
BseDI CCNNGG 4 cut(s) 305, 438, 893, 965
BseGI GGATG 4 cut(s) 431, 434, 653, 964
BseLI CCNNNNNNNGG 4 cut(s) 217, 501, 787, 825
BseMI GCAATG 2 cut(s) 1008, 1152
BseMII CTCAG 4 cut(s) 31, 234, 335, 725
BseNI ACTGG 3 cut(s) 418, 1192, 1318
BseRI GAGGAG 3 cut(s) 22, 67, 759
BseXI GCAGC 2 cut(s) 1090, 1259
BseYI CCCAGC 1 cut(s) 1029
BshFI GGCC 1 cut(s) 301
BshTI ACCGGT 1 cut(s) 196
BsiHKAI GWGCWC 1 cut(s) 32
BsiHKCI CYCGRG 1 cut(s) 1330
BsiSI CCGG 2 cut(s) 197, 495
BslFI GGGAC 5 cut(s) 247, 355, 563, 585, 883
BslI CCNNNNNNNGG 4 cut(s) 217, 501, 787, 825
BsmAI GTCTC 2 cut(s) 417, 1117
BsmFI GGGAC 5 cut(s) 247, 355, 563, 585, 883
BsmI GAATGC 1 cut(s) 147
BsnI GGCC 1 cut(s) 301
Bso31I GGTCTC 1 cut(s) 1117
BsoBI CYCGRG 1 cut(s) 1330
Bsp1286I GDGCHC 1 cut(s) 32
Bsp143I GATC 3 cut(s) 1005, 1137, 1273
Bsp19I CCATGG 1 cut(s) 965
BspACI CCGC 7 cut(s) 218, 267, 302, 443, 657, 660, 1286
BspANI GGCC 1 cut(s) 301
BspCNI CTCAG 4 cut(s) 30, 235, 334, 726
BspLI GGNNCC 2 cut(s) 768, 899
BspMAI CTGCAG 1 cut(s) 1249
BspQI GCTCTTC 2 cut(s) 690, 1071
BspTNI GGTCTC 1 cut(s) 1117
BsrBI CCGCTC 1 cut(s) 267
BsrDI GCAATG 2 cut(s) 1008, 1152
BsrFI RCCGGY 1 cut(s) 196
BsrI ACTGG 3 cut(s) 418, 1192, 1318
BssAI RCCGGY 1 cut(s) 196
BssECI CCNNGG 4 cut(s) 305, 438, 893, 965
BssMI GATC 3 cut(s) 1005, 1137, 1273
BssNI GRCGYC 1 cut(s) 91
BssT1I CCWWGG 1 cut(s) 965
Bst2UI CCWGG 3 cut(s) 603, 679, 1447
Bst4CI ACNGT 2 cut(s) 897, 1207
Bst6I CTCTTC 3 cut(s) 690, 1071, 1496
BstACI GRCGYC 1 cut(s) 91
BstC8I GCNNGC 4 cut(s) 354, 507, 1252, 1390
BstDEI CTNAG 4 cut(s) 17, 243, 321, 734
BstDSI CCRYGG 3 cut(s) 305, 893, 965
BstF5I GGATG 4 cut(s) 431, 434, 653, 964
BstKTI GATC 3 cut(s) 1008, 1140, 1276
BstMAI GTCTC 2 cut(s) 417, 1117
BstMBI GATC 3 cut(s) 1005, 1137, 1273
BstMWI GCNNNNNNNGC 2 cut(s) 36, 998
BstNI CCWGG 3 cut(s) 603, 679, 1447
BstNSI RCATGY 1 cut(s) 1375
BstSCI CCNGG 4 cut(s) 493, 601, 677, 1445
BstSFI CTRYAG 2 cut(s) 711, 1245
BstV1I GCAGC 2 cut(s) 1090, 1259
BstXI CCANNNNNNTGG 2 cut(s) 625, 996
BsuI GTATCC 1 cut(s) 1492
BsuRI GGCC 1 cut(s) 301
BtgI CCRYGG 3 cut(s) 305, 893, 965
BtsCI GGATG 4 cut(s) 431, 434, 653, 964
BtsIMutI CAGTG 1 cut(s) 1311
Cac8I GCNNGC 4 cut(s) 354, 507, 1252, 1390
CaiI CAGNNNCTG 1 cut(s) 1103
Cfr10I RCCGGY 1 cut(s) 196
Cfr13I GGNCC 3 cut(s) 208, 897, 1207
CpoI CGGWCCG 1 cut(s) 1207
CseI GACGC 1 cut(s) 1453
CsiI ACCWGGT 1 cut(s) 601
Csp6I GTAC 4 cut(s) 328, 930, 962, 1359
CspAI ACCGGT 1 cut(s) 196
CspI CGGWCCG 1 cut(s) 1207
CviAII CATG 7 cut(s) 373, 873, 924, 966, 1107, 1255, 1372
CviQI GTAC 4 cut(s) 328, 930, 962, 1359
DdeI CTNAG 4 cut(s) 17, 243, 321, 734
DpnI GATC 3 cut(s) 1007, 1139, 1275
DpnII GATC 3 cut(s) 1005, 1137, 1273
EaeI YGGCCR 1 cut(s) 299
Eam1104I CTCTTC 3 cut(s) 690, 1071, 1496
EarI CTCTTC 3 cut(s) 690, 1071, 1496
EciI GGCGGA 1 cut(s) 646
Eco130I CCWWGG 1 cut(s) 965
Eco31I GGTCTC 1 cut(s) 1117
Eco47I GGWCC 3 cut(s) 208, 897, 1207
Eco57I CTGAAG 1 cut(s) 521
Eco88I CYCGRG 1 cut(s) 1330
EcoRII CCWGG 3 cut(s) 601, 677, 1445
EcoT14I CCWWGG 1 cut(s) 965
EcoT22I ATGCAT 1 cut(s) 481
ErhI CCWWGG 1 cut(s) 965
FaeI CATG 7 cut(s) 376, 876, 927, 969, 1110, 1258, 1375
FalI AAGNNNNNCTT 4 cut(s) 107, 139, 371, 403
FaqI GGGAC 5 cut(s) 247, 355, 563, 585, 883
FatI CATG 7 cut(s) 372, 872, 923, 965, 1106, 1254, 1371
FauI CCCGC 3 cut(s) 211, 260, 436
Fnu4HI GCNGC 5 cut(s) 302, 660, 1104, 1248, 1286
FokI GGATG 4 cut(s) 421, 438, 640, 971
Fsp4HI GCNGC 5 cut(s) 302, 660, 1104, 1248, 1286
FspBI CTAG 2 cut(s) 1049, 1277
GluI GCNGC 5 cut(s) 302, 660, 1104, 1248, 1286
GsaI CCCAGC 1 cut(s) 1033
GsuI CTGGAG 1 cut(s) 1209
HaeIII GGCC 1 cut(s) 301
HapII CCGG 2 cut(s) 197, 495
HgaI GACGC 1 cut(s) 1453
Hin1I GRCGYC 1 cut(s) 91
Hin1II CATG 7 cut(s) 376, 876, 927, 969, 1110, 1258, 1375
HincII GTYRAC 1 cut(s) 552
HindII GTYRAC 1 cut(s) 552
HinfI GANTC 8 cut(s) 239, 256, 484, 797, 884, 947, 1258, 1306
HpaI GTTAAC 1 cut(s) 552
HpaII CCGG 2 cut(s) 197, 495
HphI GGTGA 6 cut(s) 72, 394, 407, 893, 956, 1368
Hpy166II GTNNAC 4 cut(s) 385, 552, 704, 1409
Hpy188I TCNGA 7 cut(s) 20, 114, 244, 324, 439, 735, 1211
Hpy188III TCNNGA 8 cut(s) 152, 260, 618, 857, 1271, 1330, 1347, 1367
Hpy8I GTNNAC 4 cut(s) 385, 552, 704, 1409
Hpy99I CGWCG 3 cut(s) 93, 96, 463
HpyAV CCTTC 4 cut(s) 635, 854, 949, 1130
HpyCH4III ACNGT 2 cut(s) 897, 1207
HpyCH4IV ACGT 2 cut(s) 91, 165
HpyCH4V TGCA 6 cut(s) 369, 479, 1106, 1157, 1247, 1254
HpyF10VI GCNNNNNNNGC 2 cut(s) 36, 998
HpyF3I CTNAG 4 cut(s) 17, 243, 321, 734
HpySE526I ACGT 2 cut(s) 91, 165
Hsp92I GRCGYC 1 cut(s) 91
Hsp92II CATG 7 cut(s) 376, 876, 927, 969, 1110, 1258, 1375
KspAI GTTAAC 1 cut(s) 552
Kzo9I GATC 3 cut(s) 1005, 1137, 1273
LguI GCTCTTC 2 cut(s) 690, 1071
LmnI GCTCC 3 cut(s) 35, 772, 1461
Lsp1109I GCAGC 2 cut(s) 1090, 1259
LweI GCATC 2 cut(s) 1166, 1443
MabI ACCWGGT 1 cut(s) 601
MaeI CTAG 2 cut(s) 1049, 1277
MaeII ACGT 2 cut(s) 91, 165
MaeIII GTNAC 3 cut(s) 944, 1144, 1374
MalI GATC 3 cut(s) 1007, 1139, 1275
MbiI CCGCTC 1 cut(s) 267
MboI GATC 3 cut(s) 1005, 1137, 1273
MfeI CAATTG 1 cut(s) 1227
MhlI GDGCHC 1 cut(s) 32
MluCI AATT 9 cut(s) 251, 402, 621, 888, 938, 1090, 1163, 1227, 1422
MlyI GAGTC 4 cut(s) 265, 493, 941, 1252
MmeI TCCRAC 1 cut(s) 235
Mph1103I ATGCAT 1 cut(s) 481
MroXI GAANNNNTTC 1 cut(s) 888
MseI TTAA 2 cut(s) 551, 1510
MslI CAYNNNNRTG 1 cut(s) 1020
MspA1I CMGCKG 1 cut(s) 992
MspI CCGG 2 cut(s) 197, 495
MspR9I CCNGG 4 cut(s) 495, 603, 679, 1447
MunI CAATTG 1 cut(s) 1227
Mva1269I GAATGC 1 cut(s) 147
MvaI CCWGG 3 cut(s) 603, 679, 1447
MwoI GCNNNNNNNGC 2 cut(s) 36, 998
NciI CCSGG 1 cut(s) 495
NcoI CCATGG 1 cut(s) 965
NdeII GATC 3 cut(s) 1005, 1137, 1273
NlaIII CATG 7 cut(s) 376, 876, 927, 969, 1110, 1258, 1375
NlaIV GGNNCC 2 cut(s) 768, 899
NmeAIII GCCGAG 1 cut(s) 49
NmuCI GTSAC 3 cut(s) 944, 1144, 1374
NsiI ATGCAT 1 cut(s) 481
NspI RCATGY 1 cut(s) 1375
PaeR7I CTCGAG 1 cut(s) 1330
PciI ACATGT 1 cut(s) 1371
PciSI GCTCTTC 2 cut(s) 690, 1071
PctI GAATGC 1 cut(s) 147
PdmI GAANNNNTTC 1 cut(s) 888
PfeI GAWTC 4 cut(s) 239, 797, 884, 1306
PflFI GACNNNGTC 1 cut(s) 90
PflMI CCANNNNNTGG 1 cut(s) 787
PinAI ACCGGT 1 cut(s) 196
PkrI GCNGC 5 cut(s) 303, 661, 1105, 1249, 1287
PleI GAGTC 4 cut(s) 264, 492, 941, 1252
PpsI GAGTC 4 cut(s) 264, 492, 941, 1252
PscI ACATGT 1 cut(s) 1371
Psp6I CCWGG 3 cut(s) 601, 677, 1445
PspFI CCCAGC 1 cut(s) 1029
PspGI CCWGG 3 cut(s) 601, 677, 1445
PspN4I GGNNCC 2 cut(s) 768, 899
PspPI GGNCC 3 cut(s) 208, 897, 1207
PstI CTGCAG 1 cut(s) 1249
PstNI CAGNNNCTG 1 cut(s) 1103
PsyI GACNNNGTC 1 cut(s) 90
PvuII CAGCTG 1 cut(s) 992
RsaI GTAC 4 cut(s) 329, 931, 963, 1360
RsaNI GTAC 4 cut(s) 328, 930, 962, 1359
RseI CAYNNNNRTG 1 cut(s) 1020
Rsr2I CGGWCCG 1 cut(s) 1207
RsrII CGGWCCG 1 cut(s) 1207
SapI GCTCTTC 2 cut(s) 690, 1071
SaqAI TTAA 2 cut(s) 551, 1510
SatI GCNGC 5 cut(s) 302, 660, 1104, 1248, 1286
Sau3AI GATC 3 cut(s) 1005, 1137, 1273
Sau96I GGNCC 3 cut(s) 208, 897, 1207
SchI GAGTC 4 cut(s) 265, 493, 941, 1252
ScrFI CCNGG 4 cut(s) 495, 603, 679, 1447
SduI GDGCHC 1 cut(s) 32
SexAI ACCWGGT 1 cut(s) 601
SfaNI GCATC 2 cut(s) 1166, 1443
SfcI CTRYAG 2 cut(s) 711, 1245
Sfr274I CTCGAG 1 cut(s) 1330
SinI GGWCC 3 cut(s) 208, 897, 1207
SlaI CTCGAG 1 cut(s) 1330
SmiMI CAYNNNNRTG 1 cut(s) 1020
SmlI CTYRAG 2 cut(s) 388, 1330
SmoI CTYRAG 2 cut(s) 388, 1330
Sse9I AATT 9 cut(s) 251, 402, 621, 888, 938, 1090, 1163, 1227, 1422
SsiI CCGC 7 cut(s) 218, 267, 302, 443, 657, 660, 1286
SspMI CTAG 2 cut(s) 1049, 1277
StyD4I CCNGG 4 cut(s) 493, 601, 677, 1445
StyI CCWWGG 1 cut(s) 965
TaaI ACNGT 2 cut(s) 897, 1207
TaiI ACGT 2 cut(s) 94, 168
TaqI TCGA 3 cut(s) 858, 1136, 1331
TasI AATT 9 cut(s) 251, 402, 621, 888, 938, 1090, 1163, 1227, 1422
TauI GCSGC 3 cut(s) 304, 662, 1288
TfiI GAWTC 4 cut(s) 239, 797, 884, 1306
Tru1I TTAA 2 cut(s) 551, 1510
Tru9I TTAA 2 cut(s) 551, 1510
TscAI CASTG 1 cut(s) 1318
TseFI GTSAC 3 cut(s) 944, 1144, 1374
TseI GCWGC 2 cut(s) 1103, 1247
Tsp45I GTSAC 3 cut(s) 944, 1144, 1374
TspDTI ATGAA 2 cut(s) 423, 1254
TspGWI ACGGA 1 cut(s) 251
TspRI CASTG 1 cut(s) 1318
Tth111I GACNNNGTC 1 cut(s) 90
Van91I CCANNNNNTGG 1 cut(s) 787
VpaK11BI GGWCC 3 cut(s) 208, 897, 1207
XapI RAATTY 3 cut(s) 621, 1090, 1422
XceI RCATGY 1 cut(s) 1375
XhoI CTCGAG 1 cut(s) 1330
XmnI GAANNNNTTC 1 cut(s) 888
XspI CTAG 2 cut(s) 1049, 1277
ZraI GACGTC 1 cut(s) 92
Zsp2I ATGCAT 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.