FvH4_1g02200

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0014627
Physical Location & Seq
Forward (+)
1178625 .. 1181065
2441 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g02200.t1

Sequence Viewer

Length: 729 bp
ATGGGGAAGAAGAGGCCACGCTCTGATGATGATCACACCCCACCCTTTTCTCCTCCTCCTCCTCCTTCGGACATCATTTCATGTTCTTCAGGAATGGAATTAGTGTCGGAAGAGAAACAATTGCACCCGGTTGATTCTGGTAAACATCTAAGGCCTCTGTCTTCTGTACTGGATACTACCGGTACTTCTTTGAAGCTACTACATGCCCACCCTTCTGCTGCACAACATCATCAGAGCCTTGGCCGATCAACGTTTTTTAAACGTTCACGTCATTACTATGCCCATCAATACTCTCGGCGTAACTCGGGCCATGTTGCCACTGCATCAACTTCTCGTGGAAAAGGTACTCCTTCACGTGAAGAAAAGCTCTCTTATAAGTTGGCTACTCAATGCAGCGCTGACTCTGGACGTCACTCAGAAATCAAGGAAAAACCCTTTTTCCGGCCGGCCAGAATTAGGTCCAGTTACTTGGTAATGGATGCTGCCTCAGCTGATCCAATGAAGATGTTATGTGGGATTTGTCAAAAGCCATTGAGGCGCAAACCTTTATTCCTTGGGACCACGCTGTCTTCTACTGAAGTCTCTGTTGTTGCAGTGTTAGTTTGCGGCCATGTTTACCATGCTGATTGTTTGGAGCAGAAAACAAGTTTTGAAGAAAGACGTGACCCTCCCTGTCCTTTGTGTGTTGGGTTGTTGCCCAAGATCGAGGATTTAAGAGAGCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_UBOX

Protein Analysis

243

Amino Acids

26.87

Weight (kDa)

9.3

Isoelectric Point (pI)

63.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 171 - 228 2.1e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014627)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02200
malus_domestica MD02G1020600.v1.1 MD15G1163400.v1.1
prunus_persica Prupe.7G250900_v2.0.a1
pyrus_communis pycom15g14700
rosa_chinensis RchiOBHm_Chr2g0087291
rosa_laevigata RLG00000015851
rosa_multiflora Rmu_sc0003367.1_g000018
rosa_roxburghii Rroxscaffold_2G00153740
rosa_rugosa Rorug01G0473700 Rorug01G0473700 Rorug01G0473700
rosa_samantha Rh2AG027600 Rh2BG026700 Rh2CG027800 Rh2DG027400
rosa_wichuraiana Rw2G002160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 375
AasI GACNNNNNNGTC 1 cut(s) 565
AatII GACGTC 1 cut(s) 412
Acc36I ACCTGC 1 cut(s) 712
AciI CCGC 1 cut(s) 606
AclI AACGTT 2 cut(s) 251, 262
AclWI GGATC 1 cut(s) 488
AcoI YGGCCR 4 cut(s) 241, 443, 447, 607
AcuI CTGAAG 2 cut(s) 72, 597
AcvI CACGTG 1 cut(s) 356
AcyI GRCGYC 1 cut(s) 409
AfaI GTAC 3 cut(s) 168, 184, 346
AfeI AGCGCT 1 cut(s) 397
AfiI CCNNNNNNNGG 2 cut(s) 441, 456
AgeI ACCGGT 1 cut(s) 179
AgsI TTSAA 2 cut(s) 193, 653
AjiI CACGTC 2 cut(s) 269, 662
AluBI AGCT 3 cut(s) 196, 367, 491
AluI AGCT 3 cut(s) 196, 367, 491
Alw26I GTCTC 1 cut(s) 586
AlwI GGATC 1 cut(s) 488
Ama87I CYCGRG 1 cut(s) 304
Aor51HI AGCGCT 1 cut(s) 397
AoxI GGCC 7 cut(s) 14, 152, 241, 307, 443, 447, 607
ApeKI GCWGC 3 cut(s) 218, 393, 482
AsiGI ACCGGT 1 cut(s) 179
AspLEI GCGC 2 cut(s) 398, 540
AspS9I GGNCC 3 cut(s) 307, 459, 558
AsuC2I CCSGG 1 cut(s) 128
AvaI CYCGRG 1 cut(s) 304
AvaII GGWCC 2 cut(s) 459, 558
BauI CACGAG 1 cut(s) 333
BbrPI CACGTG 1 cut(s) 356
BbsI GAAGAC 2 cut(s) 153, 561
BbvCI CCTCAGC 1 cut(s) 487
BbvI GCAGC 3 cut(s) 205, 405, 469
BccI CCATC 1 cut(s) 291
BciVI GTATCC 1 cut(s) 166
BclI TGATCA 1 cut(s) 31
BcnI CCSGG 1 cut(s) 128
BcoDI GTCTC 1 cut(s) 586
BfoI RGCGCY 1 cut(s) 399
BfuAI ACCTGC 1 cut(s) 712
BfuI GTATCC 1 cut(s) 166
BglI GCCNNNNNGGC 1 cut(s) 535
BisI GCNGC 4 cut(s) 219, 394, 483, 607
BlsI GCNGC 4 cut(s) 220, 395, 484, 608
Bme1390I CCNGG 1 cut(s) 128
Bme18I GGWCC 2 cut(s) 459, 558
BmeT110I CYCGRG 1 cut(s) 304
BmgBI CACGTC 2 cut(s) 269, 662
BmgT120I GGNCC 3 cut(s) 307, 459, 558
BmiI GGNNCC 1 cut(s) 559
BmrFI CCNGG 1 cut(s) 128
BmsI GCATC 2 cut(s) 332, 469
BpiI GAAGAC 2 cut(s) 153, 561
Bpu10I CCTNAGC 1 cut(s) 487
BpuMI CCSGG 1 cut(s) 128
BsaAI YACGTR 1 cut(s) 356
BsaBI GATNNNNATC 1 cut(s) 30
BsaHI GRCGYC 1 cut(s) 409
BsaJI CCNNGG 2 cut(s) 238, 553
BsaWI WCCGGW 1 cut(s) 179
Bsc4I CCNNNNNNNGG 2 cut(s) 441, 456
Bse118I RCCGGY 2 cut(s) 179, 445
Bse1I ACTGG 2 cut(s) 174, 462
Bse8I GATNNNNATC 1 cut(s) 30
BseDI CCNNGG 2 cut(s) 238, 553
BseGI GGATG 1 cut(s) 484
BseJI GATNNNNATC 1 cut(s) 30
BseLI CCNNNNNNNGG 2 cut(s) 441, 456
BseMII CTCAG 2 cut(s) 429, 501
BseNI ACTGG 2 cut(s) 174, 462
BseRI GAGGAG 4 cut(s) 42, 45, 48, 51
BseX3I CGGCCG 1 cut(s) 443
BseXI GCAGC 3 cut(s) 205, 405, 469
BsgI GTGCAG 1 cut(s) 204
Bsh1285I CGRYCG 1 cut(s) 446
BshFI GGCC 7 cut(s) 16, 154, 243, 309, 445, 449, 609
BshTI ACCGGT 1 cut(s) 179
BsiEI CGRYCG 1 cut(s) 446
BsiHKCI CYCGRG 1 cut(s) 304
BsiSI CCGG 4 cut(s) 128, 180, 442, 446
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 2 cut(s) 441, 456
BsmAI GTCTC 1 cut(s) 586
BsmFI GGGAC 1 cut(s) 571
BsnI GGCC 7 cut(s) 16, 154, 243, 309, 445, 449, 609
BsoBI CYCGRG 1 cut(s) 304
Bsp143I GATC 4 cut(s) 31, 245, 493, 702
BspACI CCGC 1 cut(s) 606
BspANI GGCC 7 cut(s) 16, 154, 243, 309, 445, 449, 609
BspCNI CTCAG 2 cut(s) 428, 500
BspLI GGNNCC 1 cut(s) 559
BspMI ACCTGC 1 cut(s) 712
BspPI GGATC 1 cut(s) 488
BsrFI RCCGGY 2 cut(s) 179, 445
BsrI ACTGG 2 cut(s) 174, 462
BssAI RCCGGY 2 cut(s) 179, 445
BssECI CCNNGG 2 cut(s) 238, 553
BssMI GATC 4 cut(s) 31, 245, 493, 702
BssNI GRCGYC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 333
BssT1I CCWWGG 2 cut(s) 238, 553
Bst2BI CACGAG 1 cut(s) 333
Bst6I CTCTTC 2 cut(s) 5, 105
BstACI GRCGYC 1 cut(s) 409
BstBAI YACGTR 1 cut(s) 356
BstC8I GCNNGC 1 cut(s) 447
BstDEI CTNAG 3 cut(s) 149, 415, 487
BstF5I GGATG 1 cut(s) 484
BstH2I RGCGCY 1 cut(s) 399
BstHHI GCGC 2 cut(s) 398, 540
BstKTI GATC 4 cut(s) 34, 248, 496, 705
BstMAI GTCTC 1 cut(s) 586
BstMBI GATC 4 cut(s) 31, 245, 493, 702
BstMCI CGRYCG 1 cut(s) 446
BstMWI GCNNNNNNNGC 2 cut(s) 488, 535
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 1 cut(s) 126
BstV1I GCAGC 3 cut(s) 205, 405, 469
BstV2I GAAGAC 2 cut(s) 153, 561
BstXI CCANNNNNNTGG 1 cut(s) 469
BstZI CGGCCG 1 cut(s) 443
BsuI GTATCC 1 cut(s) 166
BsuRI GGCC 7 cut(s) 16, 154, 243, 309, 445, 449, 609
BtrI CACGTC 2 cut(s) 269, 662
BtsCI GGATG 1 cut(s) 484
BtsI GCAGTG 2 cut(s) 318, 600
BtsIMutI CAGTG 2 cut(s) 318, 600
BveI ACCTGC 1 cut(s) 712
Cac8I GCNNGC 1 cut(s) 447
CfoI GCGC 2 cut(s) 398, 540
Cfr10I RCCGGY 2 cut(s) 179, 445
Cfr13I GGNCC 3 cut(s) 307, 459, 558
Csp6I GTAC 3 cut(s) 167, 183, 345
CspAI ACCGGT 1 cut(s) 179
CviAII CATG 5 cut(s) 81, 203, 311, 611, 620
CviQI GTAC 3 cut(s) 167, 183, 345
DdeI CTNAG 3 cut(s) 149, 415, 487
DpnI GATC 4 cut(s) 33, 247, 495, 704
DpnII GATC 4 cut(s) 31, 245, 493, 702
DraI TTTAAA 1 cut(s) 259
DrdI GACNNNNNNGTC 1 cut(s) 565
DseDI GACNNNNNNGTC 1 cut(s) 565
EaeI YGGCCR 4 cut(s) 241, 443, 447, 607
EagI CGGCCG 1 cut(s) 443
Eam1104I CTCTTC 2 cut(s) 5, 105
EarI CTCTTC 2 cut(s) 5, 105
EclXI CGGCCG 1 cut(s) 443
Eco130I CCWWGG 2 cut(s) 238, 553
Eco147I AGGCCT 1 cut(s) 154
Eco47I GGWCC 2 cut(s) 459, 558
Eco47III AGCGCT 1 cut(s) 397
Eco52I CGGCCG 1 cut(s) 443
Eco57I CTGAAG 2 cut(s) 72, 597
Eco72I CACGTG 1 cut(s) 356
Eco88I CYCGRG 1 cut(s) 304
EcoT14I CCWWGG 2 cut(s) 238, 553
ErhI CCWWGG 2 cut(s) 238, 553
FaeI CATG 5 cut(s) 84, 206, 314, 614, 623
FaiI YATR 8 cut(s) 82, 204, 279, 312, 375, 511, 612, 621
FaqI GGGAC 1 cut(s) 571
FatI CATG 5 cut(s) 80, 202, 310, 610, 619
FbaI TGATCA 1 cut(s) 31
Fnu4HI GCNGC 4 cut(s) 219, 394, 483, 607
FokI GGATG 1 cut(s) 491
FseI GGCCGGCC 1 cut(s) 449
Fsp4HI GCNGC 4 cut(s) 219, 394, 483, 607
GlaI GCGC 2 cut(s) 397, 539
GluI GCNGC 4 cut(s) 219, 394, 483, 607
HaeII RGCGCY 1 cut(s) 399
HaeIII GGCC 7 cut(s) 16, 154, 243, 309, 445, 449, 609
HapII CCGG 4 cut(s) 128, 180, 442, 446
HhaI GCGC 2 cut(s) 398, 540
Hin1I GRCGYC 1 cut(s) 409
Hin1II CATG 5 cut(s) 84, 206, 314, 614, 623
Hin6I GCGC 2 cut(s) 396, 538
HinP1I GCGC 2 cut(s) 396, 538
HinfI GANTC 2 cut(s) 134, 401
HpaII CCGG 4 cut(s) 128, 180, 442, 446
Hpy166II GTNNAC 3 cut(s) 143, 266, 616
Hpy188I TCNGA 5 cut(s) 25, 70, 109, 234, 418
Hpy188III TCNNGA 2 cut(s) 90, 405
Hpy8I GTNNAC 3 cut(s) 143, 266, 616
HpyAV CCTTC 3 cut(s) 75, 222, 360
HpyCH4IV ACGT 6 cut(s) 251, 262, 268, 355, 409, 661
HpyCH4V TGCA 5 cut(s) 124, 221, 323, 393, 593
HpyF10VI GCNNNNNNNGC 2 cut(s) 488, 535
HpyF3I CTNAG 3 cut(s) 149, 415, 487
HpySE526I ACGT 6 cut(s) 251, 262, 268, 355, 409, 661
Hsp92I GRCGYC 1 cut(s) 409
Hsp92II CATG 5 cut(s) 84, 206, 314, 614, 623
HspAI GCGC 2 cut(s) 396, 538
KroI GCCGGC 1 cut(s) 445
KroNI GCCGGC 1 cut(s) 447
Ksp22I TGATCA 1 cut(s) 31
Kzo9I GATC 4 cut(s) 31, 245, 493, 702
LmnI GCTCC 1 cut(s) 634
Lsp1109I GCAGC 3 cut(s) 205, 405, 469
LweI GCATC 2 cut(s) 332, 469
MaeII ACGT 6 cut(s) 251, 262, 268, 355, 409, 661
MaeIII GTNAC 4 cut(s) 299, 410, 464, 662
MalI GATC 4 cut(s) 33, 247, 495, 704
MboI GATC 4 cut(s) 31, 245, 493, 702
MboII GAAGA 9 cut(s) 19, 22, 78, 122, 153, 371, 514, 561, 665
MfeI CAATTG 1 cut(s) 119
MluCI AATT 3 cut(s) 98, 119, 453
MlyI GAGTC 1 cut(s) 395
MmeI TCCRAC 1 cut(s) 87
MroNI GCCGGC 1 cut(s) 445
MseI TTAA 2 cut(s) 258, 713
MslI CAYNNNNRTG 1 cut(s) 276
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 4 cut(s) 128, 180, 442, 446
MspR9I CCNGG 1 cut(s) 128
MunI CAATTG 1 cut(s) 119
MwoI GCNNNNNNNGC 2 cut(s) 488, 535
NaeI GCCGGC 1 cut(s) 447
NciI CCSGG 1 cut(s) 128
NdeII GATC 4 cut(s) 31, 245, 493, 702
NgoMIV GCCGGC 1 cut(s) 445
NlaIII CATG 5 cut(s) 84, 206, 314, 614, 623
NlaIV GGNNCC 1 cut(s) 559
NmeAIII GCCGAG 1 cut(s) 274
NmuCI GTSAC 2 cut(s) 410, 662
NspI RCATGY 1 cut(s) 206
PceI AGGCCT 1 cut(s) 154
PdiI GCCGGC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 134
PinAI ACCGGT 1 cut(s) 179
PkrI GCNGC 4 cut(s) 220, 395, 484, 608
PleI GAGTC 1 cut(s) 395
PmaCI CACGTG 1 cut(s) 356
PmlI CACGTG 1 cut(s) 356
PpsI GAGTC 1 cut(s) 395
Ppu21I YACGTR 1 cut(s) 356
PsiI TTATAA 1 cut(s) 375
Psp1406I AACGTT 2 cut(s) 251, 262
PspCI CACGTG 1 cut(s) 356
PspN4I GGNNCC 1 cut(s) 559
PspPI GGNCC 3 cut(s) 307, 459, 558
PvuII CAGCTG 1 cut(s) 491
RigI GGCCGGCC 1 cut(s) 449
RsaI GTAC 3 cut(s) 168, 184, 346
RsaNI GTAC 3 cut(s) 167, 183, 345
RseI CAYNNNNRTG 1 cut(s) 276
SaqAI TTAA 2 cut(s) 258, 713
SatI GCNGC 4 cut(s) 219, 394, 483, 607
Sau3AI GATC 4 cut(s) 31, 245, 493, 702
Sau96I GGNCC 3 cut(s) 307, 459, 558
SchI GAGTC 1 cut(s) 395
ScrFI CCNGG 1 cut(s) 128
SfaNI GCATC 2 cut(s) 332, 469
SinI GGWCC 2 cut(s) 459, 558
SmiMI CAYNNNNRTG 1 cut(s) 276
Sse9I AATT 3 cut(s) 98, 119, 453
SseBI AGGCCT 1 cut(s) 154
SsiI CCGC 1 cut(s) 606
StuI AGGCCT 1 cut(s) 154
StyD4I CCNGG 1 cut(s) 126
StyI CCWWGG 2 cut(s) 238, 553
TaiI ACGT 6 cut(s) 254, 265, 271, 358, 412, 664
TaqI TCGA 1 cut(s) 705
TasI AATT 3 cut(s) 98, 119, 453
TatI WGTACW 1 cut(s) 166
TauI GCSGC 1 cut(s) 609
TfiI GAWTC 1 cut(s) 134
Tru1I TTAA 2 cut(s) 258, 713
Tru9I TTAA 2 cut(s) 258, 713
TscAI CASTG 2 cut(s) 325, 600
TseFI GTSAC 2 cut(s) 410, 662
TseI GCWGC 3 cut(s) 218, 393, 482
Tsp45I GTSAC 2 cut(s) 410, 662
TspDTI ATGAA 2 cut(s) 69, 515
TspRI CASTG 2 cut(s) 325, 600
VpaK11BI GGWCC 2 cut(s) 459, 558
XceI RCATGY 1 cut(s) 206
ZraI GACGTC 1 cut(s) 410
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.