Rh2DG027400

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0014627
Physical Location & Seq
Forward (+)
1648175 .. 1653341
5167 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG027400.1

Sequence Viewer

Length: 636 bp
ATGGAATTGCTATCAGAAGAGAAACATTTGCACTCGATTGATTCTGGTAAACAGCTAAAGCCTCTGTCATCTGTACTGGATACTACCGGTACTTCTTTGAAGCTACTACATGCCCACCCTTCTGCTGCACAACATCATCAGAGCCTTGGCCGATCAACATTCTTTAAACGTTCACGTCATTACTATGCCCATCAATACTCTCGGCGTAACTCTGGCAGTGTTGCCAATGCATCAACTTCTCGTGGAAAAGGGACTCCTTCACGTGAAGAGAAGCTCTCTTATAAGTTGGCTACTCAATGCAGCTCTGACTCTGGACATCACTCAGAAGTGAAGGAAAAACCCTTTTTTCGGCCAGCCAGAATAAGGTCTAGTTACTTGGTAATGGATGCTGCCTCAGCTGATCCTATGAAGATGTTATGTGGGATTTGTCAGAAGCCATTGAGGCGCAAACCTTTTTTTCTTGGGATCACGCTGTCTTCTACTGAAGTCTCTGTTGTGGCAGTTTTAGTTTGCGGCCATGTTTACCATGCTGATTGTTTGGAGCAGAAAACAAGTTTTGAAGACAGACGTGACCCTCCCTGTCCTTTGTGTGTTGGGTTGTTGCCCAAGATCGAAGATTCTAGAGGGCAGGTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_UBOX

Protein Analysis

211

Amino Acids

23.45

Weight (kDa)

9.38

Isoelectric Point (pI)

53.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 140 - 197 3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014627)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02200
malus_domestica MD02G1020600.v1.1 MD15G1163400.v1.1
prunus_persica Prupe.7G250900_v2.0.a1
pyrus_communis pycom15g14700
rosa_chinensis RchiOBHm_Chr2g0087291
rosa_laevigata RLG00000015851
rosa_multiflora Rmu_sc0003367.1_g000018
rosa_roxburghii Rroxscaffold_2G00153740
rosa_rugosa Rorug01G0473700 Rorug01G0473700 Rorug01G0473700
rosa_samantha Rh2AG027600 Rh2BG026700 Rh2CG027800 Rh2DG027400
rosa_wichuraiana Rw2G002160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
Acc36I ACCTGC 1 cut(s) 619
AciI CCGC 1 cut(s) 513
AclI AACGTT 1 cut(s) 169
AclWI GGATC 2 cut(s) 395, 473
AcoI YGGCCR 3 cut(s) 148, 350, 514
AcuI CTGAAG 1 cut(s) 504
AcvI CACGTG 1 cut(s) 263
AfaI GTAC 2 cut(s) 75, 91
AfiI CCNNNNNNNGG 2 cut(s) 348, 363
AgeI ACCGGT 1 cut(s) 86
AgsI TTSAA 2 cut(s) 100, 560
AjiI CACGTC 2 cut(s) 176, 569
AluBI AGCT 5 cut(s) 55, 103, 274, 303, 398
AluI AGCT 5 cut(s) 55, 103, 274, 303, 398
Alw26I GTCTC 1 cut(s) 493
AlwI GGATC 2 cut(s) 395, 473
AoxI GGCC 3 cut(s) 148, 350, 514
ApeKI GCWGC 3 cut(s) 125, 300, 389
AsiGI ACCGGT 1 cut(s) 86
AspLEI GCGC 1 cut(s) 447
BauI CACGAG 1 cut(s) 240
BbrPI CACGTG 1 cut(s) 263
BbsI GAAGAC 2 cut(s) 468, 567
BbvCI CCTCAGC 1 cut(s) 394
BbvI GCAGC 3 cut(s) 112, 312, 376
BccI CCATC 1 cut(s) 198
BciVI GTATCC 1 cut(s) 73
BcoDI GTCTC 1 cut(s) 493
BfaI CTAG 2 cut(s) 369, 621
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 73
BglI GCCNNNNNGGC 1 cut(s) 442
BisI GCNGC 4 cut(s) 126, 301, 390, 514
BlsI GCNGC 4 cut(s) 127, 302, 391, 515
BmgBI CACGTC 2 cut(s) 176, 569
BmsI GCATC 2 cut(s) 239, 376
BpiI GAAGAC 2 cut(s) 468, 567
BplI GAGNNNNNCTC 2 cut(s) 260, 292
Bpu10I CCTNAGC 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 263
BsaJI CCNNGG 1 cut(s) 145
BsaWI WCCGGW 1 cut(s) 86
Bsc4I CCNNNNNNNGG 2 cut(s) 348, 363
Bse118I RCCGGY 1 cut(s) 86
Bse1I ACTGG 1 cut(s) 81
BseDI CCNNGG 1 cut(s) 145
BseGI GGATG 1 cut(s) 391
BseLI CCNNNNNNNGG 2 cut(s) 348, 363
BseMII CTCAG 2 cut(s) 336, 408
BseNI ACTGG 1 cut(s) 81
BseXI GCAGC 3 cut(s) 112, 312, 376
BsgI GTGCAG 1 cut(s) 111
BshFI GGCC 3 cut(s) 150, 352, 516
BshTI ACCGGT 1 cut(s) 86
BsiSI CCGG 1 cut(s) 87
BslFI GGGAC 1 cut(s) 265
BslI CCNNNNNNNGG 2 cut(s) 348, 363
BsmAI GTCTC 1 cut(s) 493
BsmFI GGGAC 1 cut(s) 265
BsnI GGCC 3 cut(s) 150, 352, 516
Bsp143I GATC 4 cut(s) 152, 400, 465, 609
BspACI CCGC 1 cut(s) 513
BspANI GGCC 3 cut(s) 150, 352, 516
BspCNI CTCAG 2 cut(s) 335, 407
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 2 cut(s) 395, 473
BsrFI RCCGGY 1 cut(s) 86
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 145
BssMI GATC 4 cut(s) 152, 400, 465, 609
BssSI CACGAG 1 cut(s) 240
BssT1I CCWWGG 1 cut(s) 145
Bst2BI CACGAG 1 cut(s) 240
Bst6I CTCTTC 2 cut(s) 12, 261
BstBAI YACGTR 1 cut(s) 263
BstC8I GCNNGC 1 cut(s) 354
BstDEI CTNAG 2 cut(s) 322, 394
BstF5I GGATG 1 cut(s) 391
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 4 cut(s) 155, 403, 468, 612
BstMAI GTCTC 1 cut(s) 493
BstMBI GATC 4 cut(s) 152, 400, 465, 609
BstMWI GCNNNNNNNGC 2 cut(s) 395, 442
BstNSI RCATGY 1 cut(s) 113
BstV1I GCAGC 3 cut(s) 112, 312, 376
BstV2I GAAGAC 2 cut(s) 468, 567
BsuI GTATCC 1 cut(s) 73
BsuRI GGCC 3 cut(s) 150, 352, 516
BtrI CACGTC 2 cut(s) 176, 569
BtsCI GGATG 1 cut(s) 391
BtsI GCAGTG 1 cut(s) 223
BtsIMutI CAGTG 1 cut(s) 223
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 1 cut(s) 354
CfoI GCGC 1 cut(s) 447
Cfr10I RCCGGY 1 cut(s) 86
Csp6I GTAC 2 cut(s) 74, 90
CspAI ACCGGT 1 cut(s) 86
CviAII CATG 3 cut(s) 110, 518, 527
CviQI GTAC 2 cut(s) 74, 90
DdeI CTNAG 2 cut(s) 322, 394
DpnI GATC 4 cut(s) 154, 402, 467, 611
DpnII GATC 4 cut(s) 152, 400, 465, 609
DraI TTTAAA 1 cut(s) 166
EaeI YGGCCR 3 cut(s) 148, 350, 514
Eam1104I CTCTTC 2 cut(s) 12, 261
EarI CTCTTC 2 cut(s) 12, 261
Eco130I CCWWGG 1 cut(s) 145
Eco57I CTGAAG 1 cut(s) 504
Eco72I CACGTG 1 cut(s) 263
EcoT14I CCWWGG 1 cut(s) 145
EcoT22I ATGCAT 1 cut(s) 232
ErhI CCWWGG 1 cut(s) 145
FaeI CATG 3 cut(s) 113, 521, 530
FaiI YATR 7 cut(s) 111, 186, 282, 407, 418, 519, 528
FaqI GGGAC 1 cut(s) 265
FatI CATG 3 cut(s) 109, 517, 526
Fnu4HI GCNGC 4 cut(s) 126, 301, 390, 514
FokI GGATG 1 cut(s) 398
Fsp4HI GCNGC 4 cut(s) 126, 301, 390, 514
FspBI CTAG 2 cut(s) 369, 621
GlaI GCGC 1 cut(s) 446
GluI GCNGC 4 cut(s) 126, 301, 390, 514
HaeIII GGCC 3 cut(s) 150, 352, 516
HapII CCGG 1 cut(s) 87
HhaI GCGC 1 cut(s) 447
Hin1II CATG 3 cut(s) 113, 521, 530
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HinfI GANTC 4 cut(s) 41, 253, 308, 617
HpaII CCGG 1 cut(s) 87
Hpy166II GTNNAC 3 cut(s) 50, 173, 523
Hpy188I TCNGA 5 cut(s) 16, 141, 307, 325, 432
Hpy188III TCNNGA 2 cut(s) 312, 621
Hpy8I GTNNAC 3 cut(s) 50, 173, 523
HpyAV CCTTC 3 cut(s) 129, 267, 325
HpyCH4IV ACGT 4 cut(s) 169, 175, 262, 568
HpyCH4V TGCA 4 cut(s) 31, 128, 230, 300
HpyF10VI GCNNNNNNNGC 2 cut(s) 395, 442
HpyF3I CTNAG 2 cut(s) 322, 394
HpySE526I ACGT 4 cut(s) 169, 175, 262, 568
Hsp92II CATG 3 cut(s) 113, 521, 530
HspAI GCGC 1 cut(s) 445
Kzo9I GATC 4 cut(s) 152, 400, 465, 609
LmnI GCTCC 1 cut(s) 541
LpnPI CCDG 9 cut(s) 30, 62, 100, 198, 297, 366, 370, 592, 614
Lsp1109I GCAGC 3 cut(s) 112, 312, 376
LweI GCATC 2 cut(s) 239, 376
MaeI CTAG 2 cut(s) 369, 621
MaeII ACGT 4 cut(s) 169, 175, 262, 568
MaeIII GTNAC 3 cut(s) 206, 371, 569
MalI GATC 4 cut(s) 154, 402, 467, 611
MboI GATC 4 cut(s) 152, 400, 465, 609
MboII GAAGA 6 cut(s) 29, 278, 421, 468, 572, 626
MluCI AATT 1 cut(s) 5
MlyI GAGTC 2 cut(s) 247, 302
MnlI CCTC 5 cut(s) 72, 403, 435, 585, 617
Mph1103I ATGCAT 1 cut(s) 232
MseI TTAA 1 cut(s) 165
MslI CAYNNNNRTG 1 cut(s) 183
MspA1I CMGCKG 1 cut(s) 398
MspI CCGG 1 cut(s) 87
MwoI GCNNNNNNNGC 2 cut(s) 395, 442
NdeII GATC 4 cut(s) 152, 400, 465, 609
NlaIII CATG 3 cut(s) 113, 521, 530
NmeAIII GCCGAG 1 cut(s) 181
NmuCI GTSAC 1 cut(s) 569
NsiI ATGCAT 1 cut(s) 232
NspI RCATGY 1 cut(s) 113
PfeI GAWTC 2 cut(s) 41, 617
PinAI ACCGGT 1 cut(s) 86
PkrI GCNGC 4 cut(s) 127, 302, 391, 515
PleI GAGTC 2 cut(s) 247, 302
PmaCI CACGTG 1 cut(s) 263
PmlI CACGTG 1 cut(s) 263
PpsI GAGTC 2 cut(s) 247, 302
Ppu21I YACGTR 1 cut(s) 263
PsiI TTATAA 1 cut(s) 282
Psp1406I AACGTT 1 cut(s) 169
PspCI CACGTG 1 cut(s) 263
PvuII CAGCTG 1 cut(s) 398
RsaI GTAC 2 cut(s) 75, 91
RsaNI GTAC 2 cut(s) 74, 90
RseI CAYNNNNRTG 1 cut(s) 183
SaqAI TTAA 1 cut(s) 165
SatI GCNGC 4 cut(s) 126, 301, 390, 514
Sau3AI GATC 4 cut(s) 152, 400, 465, 609
SchI GAGTC 2 cut(s) 247, 302
SfaNI GCATC 2 cut(s) 239, 376
SmiMI CAYNNNNRTG 1 cut(s) 183
Sse9I AATT 1 cut(s) 5
SsiI CCGC 1 cut(s) 513
SspMI CTAG 2 cut(s) 369, 621
StyI CCWWGG 1 cut(s) 145
TaiI ACGT 4 cut(s) 172, 178, 265, 571
TaqI TCGA 2 cut(s) 35, 612
TasI AATT 1 cut(s) 5
TatI WGTACW 1 cut(s) 73
TauI GCSGC 1 cut(s) 516
TfiI GAWTC 2 cut(s) 41, 617
Tru1I TTAA 1 cut(s) 165
Tru9I TTAA 1 cut(s) 165
TscAI CASTG 1 cut(s) 223
TseFI GTSAC 1 cut(s) 569
TseI GCWGC 3 cut(s) 125, 300, 389
Tsp45I GTSAC 1 cut(s) 569
TspDTI ATGAA 1 cut(s) 422
TspRI CASTG 1 cut(s) 223
XbaI TCTAGA 1 cut(s) 620
XceI RCATGY 1 cut(s) 113
XspI CTAG 2 cut(s) 369, 621
Zsp2I ATGCAT 1 cut(s) 232
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.