MD02G1020600.v1.1

Ring finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0014627
Physical Location & Seq
Reverse (-)
1461150 .. 1463551
2402 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1020600.v1.1.491

Sequence Viewer

Length: 606 bp
ATGAGCAAGAGGAAGCTCCCCTCTGATGATCACAGCCCTCCATCTTTCTCTCCTCGTTCTTCGGACACCATGCCGTGTTCTTCTGCAATGGAATTATTAACACAAGAGCATCATCCAAGCCTCGGGCGATCAATGTTCTTTAAACGCTCACGTCATCACTATGCGCACCAGTACTCTCGACGTAACTCTGGCAATCTTGCTAACCCATCAACCTCTCGCGGAAAAGGAGTGCCTTTGCGTGACGATAAGCTCTCCTATAAGTTGGCTACTCAATCCAGCTCCGAGGCCAGACGTCATTCAGAAACCAAGGAAAAGCCCTTTCTTCGGCCAGCAAGAATTAGATCTGGTTTCTTGACAACGGATGCTGTATCACCAGATCCAGGGAAGATGGTATGTGCGATTTGCGACAAGCTGTTGAGGCGTAAACCTTTCTTTCTCGGTAGCACACAGTCTTCTACTGAAGTCTCTGTTGTTGCAGTTTTAGTTTGCGGCCATCTTTTCCATGCAGATTGTTTGGAGCAGAAAACAAGTTTTGAAGATAGACACAACCCCCACTGTCCGTTGTGTGCGGGATTATTGCCCAAGGTTGAAGATTCAAGAGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-C3HC4 zf-RING_UBOX

Protein Analysis

202

Amino Acids

22.5

Weight (kDa)

9.48

Isoelectric Point (pI)

77.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-rbx1 PF12678 131 - 189 1.3e-06 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014627)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g02200
malus_domestica MD02G1020600.v1.1 MD15G1163400.v1.1
prunus_persica Prupe.7G250900_v2.0.a1
pyrus_communis pycom15g14700
rosa_chinensis RchiOBHm_Chr2g0087291
rosa_laevigata RLG00000015851
rosa_multiflora Rmu_sc0003367.1_g000018
rosa_roxburghii Rroxscaffold_2G00153740
rosa_rugosa Rorug01G0473700 Rorug01G0473700 Rorug01G0473700
rosa_samantha Rh2AG027600 Rh2BG026700 Rh2CG027800 Rh2DG027400
rosa_wichuraiana Rw2G002160

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 295
Acc16I TGCGCA 1 cut(s) 165
AccII CGCG 1 cut(s) 219
AciI CCGC 3 cut(s) 219, 489, 569
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 2 cut(s) 326, 490
AcuI CTGAAG 1 cut(s) 480
AcyI GRCGYC 1 cut(s) 292
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 122, 324, 380
AgsI TTSAA 3 cut(s) 536, 590, 597
AjiI CACGTC 1 cut(s) 152
AjnI CCWGG 1 cut(s) 379
AluBI AGCT 4 cut(s) 16, 250, 279, 412
AluI AGCT 4 cut(s) 16, 250, 279, 412
Alw26I GTCTC 1 cut(s) 469
AlwI GGATC 1 cut(s) 371
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 3 cut(s) 285, 326, 490
AspLEI GCGC 1 cut(s) 166
AsuHPI GGTGA 1 cut(s) 363
AvaI CYCGRG 1 cut(s) 122
BbsI GAAGAC 1 cut(s) 444
BccI CCATC 4 cut(s) 49, 214, 382, 501
BceAI ACGGC 1 cut(s) 58
BciT130I CCWGG 1 cut(s) 381
BclI TGATCA 1 cut(s) 28
BcoDI GTCTC 1 cut(s) 469
BglII AGATCT 1 cut(s) 341
BisI GCNGC 1 cut(s) 490
BlsI GCNGC 1 cut(s) 491
BmcAI AGTACT 1 cut(s) 173
Bme1390I CCNGG 1 cut(s) 381
BmeT110I CYCGRG 1 cut(s) 122
BmgBI CACGTC 1 cut(s) 152
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 2 cut(s) 118, 352
BpiI GAAGAC 1 cut(s) 444
BsaHI GRCGYC 1 cut(s) 292
BsaJI CCNNGG 5 cut(s) 121, 282, 306, 380, 582
Bsc4I CCNNNNNNNGG 3 cut(s) 122, 324, 380
Bse1I ACTGG 1 cut(s) 169
Bse3DI GCAATG 1 cut(s) 93
BseBI CCWGG 1 cut(s) 381
BseDI CCNNGG 5 cut(s) 121, 282, 306, 380, 582
BseGI GGATG 2 cut(s) 112, 367
BseLI CCNNNNNNNGG 3 cut(s) 122, 324, 380
BseMI GCAATG 1 cut(s) 93
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 219
BshFI GGCC 3 cut(s) 287, 328, 492
BsiHKCI CYCGRG 1 cut(s) 122
BslI CCNNNNNNNGG 3 cut(s) 122, 324, 380
BsmAI GTCTC 1 cut(s) 469
BsnI GGCC 3 cut(s) 287, 328, 492
BsoBI CYCGRG 1 cut(s) 122
Bsp143I GATC 4 cut(s) 28, 128, 341, 376
BspACI CCGC 3 cut(s) 219, 489, 569
BspANI GGCC 3 cut(s) 287, 328, 492
BspFNI CGCG 1 cut(s) 219
BspPI GGATC 1 cut(s) 371
BsrDI GCAATG 1 cut(s) 93
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 5 cut(s) 121, 282, 306, 380, 582
BssMI GATC 4 cut(s) 28, 128, 341, 376
BssNI GRCGYC 1 cut(s) 292
BssT1I CCWWGG 2 cut(s) 306, 582
Bst2UI CCWGG 1 cut(s) 381
Bst4CI ACNGT 2 cut(s) 450, 557
BstACI GRCGYC 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 330
BstF5I GGATG 2 cut(s) 112, 367
BstFNI CGCG 1 cut(s) 219
BstHHI GCGC 1 cut(s) 166
BstKTI GATC 4 cut(s) 31, 131, 344, 379
BstMAI GTCTC 1 cut(s) 469
BstMBI GATC 4 cut(s) 28, 128, 341, 376
BstMWI GCNNNNNNNGC 1 cut(s) 418
BstNI CCWGG 1 cut(s) 381
BstSCI CCNGG 1 cut(s) 379
BstUI CGCG 1 cut(s) 219
BstV2I GAAGAC 1 cut(s) 444
BstX2I RGATCY 2 cut(s) 341, 376
BstYI RGATCY 2 cut(s) 341, 376
BsuRI GGCC 3 cut(s) 287, 328, 492
BtrI CACGTC 1 cut(s) 152
BtsCI GGATG 2 cut(s) 112, 367
BtsIMutI CAGTG 1 cut(s) 553
Cac8I GCNNGC 1 cut(s) 330
CfoI GCGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 172
CviAII CATG 2 cut(s) 70, 503
CviQI GTAC 1 cut(s) 172
DpnI GATC 4 cut(s) 30, 130, 343, 378
DpnII GATC 4 cut(s) 28, 128, 341, 376
DraI TTTAAA 1 cut(s) 142
EaeI YGGCCR 2 cut(s) 326, 490
Eco130I CCWWGG 2 cut(s) 306, 582
Eco57I CTGAAG 1 cut(s) 480
Eco88I CYCGRG 1 cut(s) 122
EcoRII CCWGG 1 cut(s) 379
EcoT14I CCWWGG 2 cut(s) 306, 582
ErhI CCWWGG 2 cut(s) 306, 582
FaeI CATG 2 cut(s) 73, 506
FaiI YATR 5 cut(s) 71, 162, 258, 394, 504
FatI CATG 2 cut(s) 69, 502
FauI CCCGC 1 cut(s) 562
FbaI TGATCA 1 cut(s) 28
Fnu4HI GCNGC 1 cut(s) 490
FokI GGATG 2 cut(s) 99, 374
Fsp4HI GCNGC 1 cut(s) 490
FspAI RTGCGCAY 1 cut(s) 165
FspI TGCGCA 1 cut(s) 165
GlaI GCGC 1 cut(s) 165
GluI GCNGC 1 cut(s) 490
HaeIII GGCC 3 cut(s) 287, 328, 492
HhaI GCGC 1 cut(s) 166
Hin1I GRCGYC 1 cut(s) 292
Hin1II CATG 2 cut(s) 73, 506
Hin6I GCGC 1 cut(s) 164
HinP1I GCGC 1 cut(s) 164
HinfI GANTC 1 cut(s) 593
HphI GGTGA 1 cut(s) 363
Hpy166II GTNNAC 1 cut(s) 425
Hpy188I TCNGA 4 cut(s) 25, 64, 283, 301
Hpy188III TCNNGA 3 cut(s) 177, 352, 597
Hpy8I GTNNAC 1 cut(s) 425
Hpy99I CGWCG 1 cut(s) 183
HpyCH4III ACNGT 2 cut(s) 450, 557
HpyCH4IV ACGT 3 cut(s) 151, 181, 292
HpyCH4V TGCA 3 cut(s) 86, 476, 506
HpyF10VI GCNNNNNNNGC 1 cut(s) 418
HpySE526I ACGT 3 cut(s) 151, 181, 292
Hsp92I GRCGYC 1 cut(s) 292
Hsp92II CATG 2 cut(s) 73, 506
HspAI GCGC 1 cut(s) 164
Ksp22I TGATCA 1 cut(s) 28
Kzo9I GATC 4 cut(s) 28, 128, 341, 376
LmnI GCTCC 3 cut(s) 21, 284, 517
LpnPI CCDG 9 cut(s) 174, 182, 289, 301, 330, 342, 366, 387, 393
LweI GCATC 2 cut(s) 118, 352
MaeII ACGT 3 cut(s) 151, 181, 292
MaeIII GTNAC 2 cut(s) 182, 239
MalI GATC 4 cut(s) 30, 130, 343, 378
MboI GATC 4 cut(s) 28, 128, 341, 376
MboII GAAGA 7 cut(s) 51, 72, 314, 397, 444, 548, 602
MflI RGATCY 2 cut(s) 341, 376
MluCI AATT 2 cut(s) 92, 336
MnlI CCTC 8 cut(s) 3, 31, 48, 63, 131, 223, 277, 411
MseI TTAA 2 cut(s) 98, 141
MslI CAYNNNNRTG 1 cut(s) 159
MspR9I CCNGG 1 cut(s) 381
MvaI CCWGG 1 cut(s) 381
MvnI CGCG 1 cut(s) 219
MwoI GCNNNNNNNGC 1 cut(s) 418
NdeII GATC 4 cut(s) 28, 128, 341, 376
NlaIII CATG 2 cut(s) 73, 506
NmuCI GTSAC 1 cut(s) 239
NsbI TGCGCA 1 cut(s) 165
PfeI GAWTC 1 cut(s) 593
PkrI GCNGC 1 cut(s) 491
Psp6I CCWGG 1 cut(s) 379
PspGI CCWGG 1 cut(s) 379
PsuI RGATCY 2 cut(s) 341, 376
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 1 cut(s) 159
SaqAI TTAA 2 cut(s) 98, 141
SatI GCNGC 1 cut(s) 490
Sau3AI GATC 4 cut(s) 28, 128, 341, 376
ScaI AGTACT 1 cut(s) 173
ScrFI CCNGG 1 cut(s) 381
SfaNI GCATC 2 cut(s) 118, 352
SmiMI CAYNNNNRTG 1 cut(s) 159
Sse9I AATT 2 cut(s) 92, 336
SsiI CCGC 3 cut(s) 219, 489, 569
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 2 cut(s) 306, 582
TaaI ACNGT 2 cut(s) 450, 557
TaiI ACGT 3 cut(s) 154, 184, 295
TaqI TCGA 1 cut(s) 178
TasI AATT 2 cut(s) 92, 336
TatI WGTACW 1 cut(s) 171
TauI GCSGC 1 cut(s) 492
TfiI GAWTC 1 cut(s) 593
Tru1I TTAA 2 cut(s) 98, 141
Tru9I TTAA 2 cut(s) 98, 141
TscAI CASTG 1 cut(s) 560
TseFI GTSAC 1 cut(s) 239
Tsp45I GTSAC 1 cut(s) 239
TspGWI ACGGA 2 cut(s) 374, 549
TspRI CASTG 1 cut(s) 560
ZraI GACGTC 1 cut(s) 293
ZrmI AGTACT 1 cut(s) 173
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.