FvH4_1g05270

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0017193
Physical Location & Seq
Forward (+)
2802340 .. 2804778
2439 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g05270.t1

Sequence Viewer

Length: 477 bp
ATGACGATACCTCTCCTCTTCTTCTCCGCCCTTACCCTCCTCGTTTCTCTCTCCTCCCAAACGGCCGACCCGAAACCCCCAAACCCGACCCGAAGCCCATCGCCGGCCCACACCGAACTCACCCGCTTCGGCTTCCCGATCGGGCTCCTCCCTTCCCCGGTGGAAAACTACACCGTCAACCCCCTCACCGGCGACTTCGTCGTCGACCTCGGCGACGCGTGCAAGATCACGCTCCCGCCGGACAACTACCTCGCCACGTATTCCAAGAGAGTCACCGGCAAAATCAACCACGGCCGGATCGCCGAGATCAACGGCATCAGCGTCCGCGCTTTCTACCAGTGGTGGTCCATCACCGGAATCAGGTCCAGCGGCGAGAATCTGGTGTTTGAGGTCGGCATGGTGACGGCGAAGTATCCGTCTAAGAACTTCGTTGACAGTCCGGCCTGTGAGGGCCGCCGCCGCCACTCCGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

159

Amino Acids

17.27

Weight (kDa)

9.0

Isoelectric Point (pI)

51.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 37 - 143 8.3e-27 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19590 AT5G19590
fragaria_vesca FvH4_1g05270
malus_domestica MD02G1056100.v1.1 MD15G1188900.v1.1
prunus_persica Prupe.7G227700_v2.0.a1 Prupe.7G227700_v2.0.a1
pyrus_communis pycom02g04510
rosa_chinensis RchiOBHm_Chr2g0090991
rosa_laevigata RLG00000016164
rosa_multiflora Rmu_sc0000792.1_g000022 Rmu_sc0003305.1_g000003
rosa_wichuraiana Rw2G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 200
AccI GTMKAC 1 cut(s) 204
AccII CGCG 2 cut(s) 218, 327
AciI CCGC 9 cut(s) 27, 124, 236, 325, 369, 454, 457, 460, 468
AclWI GGATC 1 cut(s) 305
AcoI YGGCCR 2 cut(s) 63, 292
AfiI CCNNNNNNNGG 4 cut(s) 103, 157, 188, 360
AflIII ACRYGT 1 cut(s) 216
AlwI GGATC 1 cut(s) 305
AoxI GGCC 5 cut(s) 63, 105, 292, 441, 451
ArsI GACNNNNNNTTYG 2 cut(s) 401, 433
AspLEI GCGC 1 cut(s) 329
AspS9I GGNCC 4 cut(s) 106, 345, 363, 451
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 5 cut(s) 112, 178, 265, 343, 412
AvaII GGWCC 2 cut(s) 345, 363
BanII GRGCYC 1 cut(s) 147
BccI CCATC 2 cut(s) 106, 356
BceAI ACGGC 4 cut(s) 78, 307, 328, 420
BciVI GTATCC 1 cut(s) 423
BcnI CCSGG 1 cut(s) 158
BfuI GTATCC 1 cut(s) 423
BisI GCNGC 4 cut(s) 370, 454, 457, 460
BlsI GCNGC 4 cut(s) 371, 455, 458, 461
Bme1390I CCNGG 1 cut(s) 158
Bme18I GGWCC 2 cut(s) 345, 363
BmgT120I GGNCC 4 cut(s) 106, 345, 363, 451
BmiI GGNNCC 1 cut(s) 146
BmrFI CCNGG 1 cut(s) 158
BmsI GCATC 1 cut(s) 324
BpuMI CCSGG 1 cut(s) 158
BsaAI YACGTR 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 156, 208, 289
BsaWI WCCGGW 1 cut(s) 353
Bsc4I CCNNNNNNNGG 4 cut(s) 103, 157, 188, 360
Bse118I RCCGGY 3 cut(s) 103, 188, 275
Bse1I ACTGG 1 cut(s) 337
BseDI CCNNGG 3 cut(s) 156, 208, 289
BseLI CCNNNNNNNGG 4 cut(s) 103, 157, 188, 360
BseNI ACTGG 1 cut(s) 337
BseRI GAGGAG 4 cut(s) 5, 29, 43, 137
BseX3I CGGCCG 2 cut(s) 63, 292
Bsh1236I CGCG 2 cut(s) 218, 327
Bsh1285I CGRYCG 3 cut(s) 66, 141, 295
BshFI GGCC 5 cut(s) 65, 107, 294, 443, 453
BsiEI CGRYCG 3 cut(s) 66, 141, 295
BsiSI CCGG 8 cut(s) 104, 158, 189, 239, 276, 295, 354, 440
BslI CCNNNNNNNGG 4 cut(s) 103, 157, 188, 360
BsnI GGCC 5 cut(s) 65, 107, 294, 443, 453
Bsp1286I GDGCHC 1 cut(s) 147
Bsp143I GATC 4 cut(s) 138, 225, 297, 306
BspACI CCGC 9 cut(s) 27, 124, 236, 325, 369, 454, 457, 460, 468
BspANI GGCC 5 cut(s) 65, 107, 294, 443, 453
BspFNI CGCG 2 cut(s) 218, 327
BspLI GGNNCC 1 cut(s) 146
BspPI GGATC 1 cut(s) 305
BsrFI RCCGGY 3 cut(s) 103, 188, 275
BsrI ACTGG 1 cut(s) 337
BssAI RCCGGY 3 cut(s) 103, 188, 275
BssECI CCNNGG 3 cut(s) 156, 208, 289
BssMI GATC 4 cut(s) 138, 225, 297, 306
Bst4CI ACNGT 2 cut(s) 175, 437
Bst6I CTCTTC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 258
BstC8I GCNNGC 2 cut(s) 105, 220
BstDEI CTNAG 1 cut(s) 420
BstDSI CCRYGG 1 cut(s) 289
BstFNI CGCG 2 cut(s) 218, 327
BstHHI GCGC 1 cut(s) 329
BstKTI GATC 4 cut(s) 141, 228, 300, 309
BstMBI GATC 4 cut(s) 138, 225, 297, 306
BstMCI CGRYCG 3 cut(s) 66, 141, 295
BstMWI GCNNNNNNNGC 2 cut(s) 219, 459
BstSCI CCNGG 1 cut(s) 156
BstUI CGCG 2 cut(s) 218, 327
BstZI CGGCCG 2 cut(s) 63, 292
BsuI GTATCC 1 cut(s) 423
BsuRI GGCC 5 cut(s) 65, 107, 294, 443, 453
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 84
BtsIMutI CAGTG 1 cut(s) 344
Cac8I GCNNGC 2 cut(s) 105, 220
CfoI GCGC 1 cut(s) 329
Cfr10I RCCGGY 3 cut(s) 103, 188, 275
Cfr13I GGNCC 4 cut(s) 106, 345, 363, 451
CseI GACGC 2 cut(s) 224, 310
CviAII CATG 1 cut(s) 397
CviJI RGCY 8 cut(s) 65, 96, 107, 132, 145, 294, 443, 453
CviKI_1 RGCY 8 cut(s) 65, 96, 107, 132, 145, 294, 443, 453
DdeI CTNAG 1 cut(s) 420
DpnI GATC 4 cut(s) 140, 227, 299, 308
DpnII GATC 4 cut(s) 138, 225, 297, 306
DrdI GACNNNNNNGTC 1 cut(s) 200
DseDI GACNNNNNNGTC 1 cut(s) 200
EaeI YGGCCR 2 cut(s) 63, 292
EagI CGGCCG 2 cut(s) 63, 292
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EciI GGCGGA 1 cut(s) 16
EclXI CGGCCG 2 cut(s) 63, 292
Eco24I GRGCYC 1 cut(s) 147
Eco47I GGWCC 2 cut(s) 345, 363
Eco52I CGGCCG 2 cut(s) 63, 292
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 1 cut(s) 400
FaiI YATR 1 cut(s) 398
FatI CATG 1 cut(s) 396
FauI CCCGC 2 cut(s) 131, 243
FblI GTMKAC 1 cut(s) 204
Fnu4HI GCNGC 4 cut(s) 370, 454, 457, 460
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 4 cut(s) 370, 454, 457, 460
GlaI GCGC 1 cut(s) 328
GluI GCNGC 4 cut(s) 370, 454, 457, 460
HaeIII GGCC 5 cut(s) 65, 107, 294, 443, 453
HapII CCGG 8 cut(s) 104, 158, 189, 239, 276, 295, 354, 440
HgaI GACGC 2 cut(s) 224, 310
HhaI GCGC 1 cut(s) 329
Hin1II CATG 1 cut(s) 400
Hin6I GCGC 1 cut(s) 327
HinP1I GCGC 1 cut(s) 327
HincII GTYRAC 3 cut(s) 178, 205, 433
HindII GTYRAC 3 cut(s) 178, 205, 433
HinfI GANTC 3 cut(s) 270, 357, 376
HpaII CCGG 8 cut(s) 104, 158, 189, 239, 276, 295, 354, 440
HphI GGTGA 5 cut(s) 112, 178, 265, 343, 412
Hpy166II GTNNAC 3 cut(s) 178, 205, 433
Hpy188III TCNNGA 1 cut(s) 136
Hpy8I GTNNAC 3 cut(s) 178, 205, 433
Hpy99I CGWCG 3 cut(s) 203, 206, 218
HpyAV CCTTC 1 cut(s) 162
HpyCH4III ACNGT 2 cut(s) 175, 437
HpyCH4IV ACGT 1 cut(s) 257
HpyCH4V TGCA 1 cut(s) 222
HpyF10VI GCNNNNNNNGC 2 cut(s) 219, 459
HpyF3I CTNAG 1 cut(s) 420
HpySE526I ACGT 1 cut(s) 257
Hsp92II CATG 1 cut(s) 400
HspAI GCGC 1 cut(s) 327
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 4 cut(s) 138, 225, 297, 306
LmnI GCTCC 2 cut(s) 150, 237
LweI GCATC 1 cut(s) 324
MaeII ACGT 1 cut(s) 257
MaeIII GTNAC 2 cut(s) 271, 400
MalI GATC 4 cut(s) 140, 227, 299, 308
MboI GATC 4 cut(s) 138, 225, 297, 306
MboII GAAGA 2 cut(s) 10, 13
MhlI GDGCHC 1 cut(s) 147
MluI ACGCGT 1 cut(s) 216
MlyI GAGTC 1 cut(s) 279
MroNI GCCGGC 1 cut(s) 103
MseI TTAA 1 cut(s) 475
MspA1I CMGCKG 1 cut(s) 369
MspI CCGG 8 cut(s) 104, 158, 189, 239, 276, 295, 354, 440
MspR9I CCNGG 1 cut(s) 158
MvnI CGCG 2 cut(s) 218, 327
MwoI GCNNNNNNNGC 2 cut(s) 219, 459
NaeI GCCGGC 1 cut(s) 105
NciI CCSGG 1 cut(s) 158
NdeII GATC 4 cut(s) 138, 225, 297, 306
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 1 cut(s) 400
NlaIV GGNNCC 1 cut(s) 146
NmeAIII GCCGAG 2 cut(s) 189, 328
NmuCI GTSAC 2 cut(s) 271, 400
PcsI WCGNNNNNNNCGW 3 cut(s) 68, 210, 318
PdiI GCCGGC 1 cut(s) 105
PfeI GAWTC 2 cut(s) 357, 376
PflFI GACNNNGTC 1 cut(s) 197
PkrI GCNGC 4 cut(s) 371, 455, 458, 461
Ple19I CGATCG 1 cut(s) 141
PleI GAGTC 1 cut(s) 278
PpsI GAGTC 1 cut(s) 278
Ppu21I YACGTR 1 cut(s) 258
PspN4I GGNNCC 1 cut(s) 146
PspPI GGNCC 4 cut(s) 106, 345, 363, 451
PsyI GACNNNGTC 1 cut(s) 197
PvuI CGATCG 1 cut(s) 141
SalI GTCGAC 1 cut(s) 203
SaqAI TTAA 1 cut(s) 475
SatI GCNGC 4 cut(s) 370, 454, 457, 460
Sau3AI GATC 4 cut(s) 138, 225, 297, 306
Sau96I GGNCC 4 cut(s) 106, 345, 363, 451
SchI GAGTC 1 cut(s) 279
ScrFI CCNGG 1 cut(s) 158
SduI GDGCHC 1 cut(s) 147
SetI ASST 6 cut(s) 13, 210, 252, 260, 365, 393
SfaNI GCATC 1 cut(s) 324
SgrAI CRCCGGYG 1 cut(s) 188
SinI GGWCC 2 cut(s) 345, 363
SsiI CCGC 9 cut(s) 27, 124, 236, 325, 369, 454, 457, 460, 468
StyD4I CCNGG 1 cut(s) 156
TaaI ACNGT 2 cut(s) 175, 437
TaiI ACGT 1 cut(s) 260
TaqI TCGA 1 cut(s) 204
TauI GCSGC 4 cut(s) 372, 456, 459, 462
TfiI GAWTC 2 cut(s) 357, 376
Tru1I TTAA 1 cut(s) 475
Tru9I TTAA 1 cut(s) 475
TscAI CASTG 1 cut(s) 344
TseFI GTSAC 2 cut(s) 271, 400
Tsp45I GTSAC 2 cut(s) 271, 400
TspGWI ACGGA 1 cut(s) 405
TspRI CASTG 1 cut(s) 344
Tth111I GACNNNGTC 1 cut(s) 197
VpaK11BI GGWCC 2 cut(s) 345, 363
XmiI GTMKAC 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.