MD15G1188900.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0017193
Physical Location & Seq
Forward (+)
14920998 .. 14921498
501 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1188900.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCAAAATCTCTCTTCGTCTGCATCTCTCTCCTCCTCCTCCTCCGCCTCACCTCCCAAACCACTGACCCGAGCACCCCGAACCGAAACTCAATTTCAAGCCCATCTCCGGCCCACACCGAGCTCACCAACTACGGCTTCCCAATTGGCCTCCTCCCCACCGCCGTCAAGAACTACACCATCAACCGCACCACCGGCGACTTCATCGTCGACCTCGGCGGCGCGTGCAAGGTAACCCTCCCTCCTGACAACTACCTGGCCACCTACTCGAAGAAAATCACCGGAAAGATCGTCGAGGGCAAAATCGCTGAGATCAACGGCATCAGCGTCCGGGCCTTCTTCCAGTGGTGGTCGATCACCGGAATTCGGTCGAGCGGCGACAACTTGGTGTTCGAGGTCGGCATGGTCTCCGCCAAGTACCCATCTAAGAACTTCGACGAGAGCCCTGCTTGCGAGGGCCGCCACTCCTCTTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

159

Amino Acids

17.12

Weight (kDa)

8.81

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 39 - 145 2.1e-29 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19590 AT5G19590
fragaria_vesca FvH4_1g05270
malus_domestica MD02G1056100.v1.1 MD15G1188900.v1.1
prunus_persica Prupe.7G227700_v2.0.a1 Prupe.7G227700_v2.0.a1
pyrus_communis pycom02g04510
rosa_chinensis RchiOBHm_Chr2g0090991
rosa_laevigata RLG00000016164
rosa_multiflora Rmu_sc0000792.1_g000022 Rmu_sc0003305.1_g000003
rosa_wichuraiana Rw2G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 206
AccBSI CCGCTC 1 cut(s) 375
AccI GTMKAC 1 cut(s) 210
AccII CGCG 1 cut(s) 224
AciI CCGC 7 cut(s) 46, 162, 187, 219, 375, 411, 460
AcoI YGGCCR 1 cut(s) 258
AcsI RAATTY 1 cut(s) 363
AfaI GTAC 1 cut(s) 419
AfiI CCNNNNNNNGG 2 cut(s) 109, 366
AgsI TTSAA 1 cut(s) 99
AjnI CCWGG 1 cut(s) 255
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AluBI AGCT 1 cut(s) 124
AluI AGCT 1 cut(s) 124
Alw21I GWGCWC 2 cut(s) 77, 126
Alw26I GTCTC 1 cut(s) 412
Ama87I CYCGRG 1 cut(s) 70
AoxI GGCC 5 cut(s) 111, 148, 258, 333, 457
ApoI RAATTY 1 cut(s) 363
AspLEI GCGC 1 cut(s) 224
AspS9I GGNCC 3 cut(s) 112, 333, 457
AsuC2I CCSGG 1 cut(s) 332
AsuHPI GGTGA 4 cut(s) 43, 118, 271, 349
AvaI CYCGRG 1 cut(s) 70
BalI TGGCCA 1 cut(s) 260
BanII GRGCYC 2 cut(s) 126, 446
Bbv12I GWGCWC 2 cut(s) 77, 126
BccI CCATC 3 cut(s) 112, 188, 430
BceAI ACGGC 3 cut(s) 149, 151, 334
BcgI CGANNNNNNTGC 2 cut(s) 428, 462
BciT130I CCWGG 1 cut(s) 257
BcnI CCSGG 1 cut(s) 332
BcoDI GTCTC 1 cut(s) 412
BisI GCNGC 3 cut(s) 220, 376, 460
BlsI GCNGC 3 cut(s) 221, 377, 461
Bme1390I CCNGG 2 cut(s) 257, 332
BmeT110I CYCGRG 1 cut(s) 70
BmgT120I GGNCC 3 cut(s) 112, 333, 457
BmrFI CCNGG 2 cut(s) 257, 332
BmsI GCATC 2 cut(s) 33, 330
BpuMI CCSGG 1 cut(s) 332
BsaI GGTCTC 1 cut(s) 412
BsaJI CCNNGG 1 cut(s) 214
BsaWI WCCGGW 2 cut(s) 281, 359
BsaXI ACNNNNNCTCC 2 cut(s) 226, 256
Bsc4I CCNNNNNNNGG 2 cut(s) 109, 366
Bse118I RCCGGY 1 cut(s) 194
Bse1I ACTGG 1 cut(s) 343
BseBI CCWGG 1 cut(s) 257
BseDI CCNNGG 1 cut(s) 214
BseLI CCNNNNNNNGG 2 cut(s) 109, 366
BseMII CTCAG 1 cut(s) 300
BseNI ACTGG 1 cut(s) 343
BseRI GAGGAG 6 cut(s) 23, 26, 29, 32, 143, 457
Bsh1236I CGCG 1 cut(s) 224
Bsh1285I CGRYCG 1 cut(s) 371
BshFI GGCC 5 cut(s) 113, 150, 260, 335, 459
BsiEI CGRYCG 1 cut(s) 371
BsiHKAI GWGCWC 2 cut(s) 77, 126
BsiHKCI CYCGRG 1 cut(s) 70
BsiSI CCGG 5 cut(s) 110, 195, 282, 331, 360
BslI CCNNNNNNNGG 2 cut(s) 109, 366
BsmAI GTCTC 1 cut(s) 412
BsnI GGCC 5 cut(s) 113, 150, 260, 335, 459
Bso31I GGTCTC 1 cut(s) 412
BsoBI CYCGRG 1 cut(s) 70
Bsp1286I GDGCHC 3 cut(s) 77, 126, 446
Bsp143I GATC 3 cut(s) 288, 312, 354
BspACI CCGC 7 cut(s) 46, 162, 187, 219, 375, 411, 460
BspANI GGCC 5 cut(s) 113, 150, 260, 335, 459
BspCNI CTCAG 1 cut(s) 301
BspFNI CGCG 1 cut(s) 224
BspTNI GGTCTC 1 cut(s) 412
BsrBI CCGCTC 1 cut(s) 375
BsrFI RCCGGY 1 cut(s) 194
BsrI ACTGG 1 cut(s) 343
BssAI RCCGGY 1 cut(s) 194
BssECI CCNNGG 1 cut(s) 214
BssMI GATC 3 cut(s) 288, 312, 354
Bst2UI CCWGG 1 cut(s) 257
Bst6I CTCTTC 1 cut(s) 20
BstC8I GCNNGC 2 cut(s) 226, 451
BstDEI CTNAG 3 cut(s) 309, 426, 474
BstEII GGTNACC 1 cut(s) 232
BstFNI CGCG 1 cut(s) 224
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 3 cut(s) 291, 315, 357
BstMAI GTCTC 1 cut(s) 412
BstMBI GATC 3 cut(s) 288, 312, 354
BstMCI CGRYCG 1 cut(s) 371
BstMWI GCNNNNNNNGC 4 cut(s) 195, 225, 450, 459
BstNI CCWGG 1 cut(s) 257
BstPI GGTNACC 1 cut(s) 232
BstSCI CCNGG 2 cut(s) 255, 330
BstUI CGCG 1 cut(s) 224
BsuRI GGCC 5 cut(s) 113, 150, 260, 335, 459
BtsIMutI CAGTG 2 cut(s) 63, 350
Cac8I GCNNGC 2 cut(s) 226, 451
CfoI GCGC 1 cut(s) 224
Cfr10I RCCGGY 1 cut(s) 194
Cfr13I GGNCC 3 cut(s) 112, 333, 457
CseI GACGC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 418
CviAII CATG 1 cut(s) 403
CviJI RGCY 9 cut(s) 102, 113, 124, 138, 150, 260, 335, 444, 459
CviKI_1 RGCY 9 cut(s) 102, 113, 124, 138, 150, 260, 335, 444, 459
CviQI GTAC 1 cut(s) 418
DdeI CTNAG 3 cut(s) 309, 426, 474
DpnI GATC 3 cut(s) 290, 314, 356
DpnII GATC 3 cut(s) 288, 312, 354
DrdI GACNNNNNNGTC 1 cut(s) 206
DseDI GACNNNNNNGTC 1 cut(s) 206
EaeI YGGCCR 1 cut(s) 258
Eam1104I CTCTTC 1 cut(s) 20
EarI CTCTTC 1 cut(s) 20
EciI GGCGGA 2 cut(s) 35, 400
Ecl136II GAGCTC 1 cut(s) 124
Eco24I GRGCYC 2 cut(s) 126, 446
Eco31I GGTCTC 1 cut(s) 412
Eco53kI GAGCTC 1 cut(s) 124
Eco88I CYCGRG 1 cut(s) 70
Eco91I GGTNACC 1 cut(s) 232
EcoICRI GAGCTC 1 cut(s) 124
EcoO65I GGTNACC 1 cut(s) 232
EcoRI GAATTC 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 255
EcoT38I GRGCYC 2 cut(s) 126, 446
FaeI CATG 1 cut(s) 406
FaiI YATR 1 cut(s) 404
FatI CATG 1 cut(s) 402
FblI GTMKAC 1 cut(s) 210
Fnu4HI GCNGC 3 cut(s) 220, 376, 460
FriOI GRGCYC 2 cut(s) 126, 446
Fsp4HI GCNGC 3 cut(s) 220, 376, 460
GlaI GCGC 1 cut(s) 223
GluI GCNGC 3 cut(s) 220, 376, 460
HaeIII GGCC 5 cut(s) 113, 150, 260, 335, 459
HapII CCGG 5 cut(s) 110, 195, 282, 331, 360
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 224
Hin1II CATG 1 cut(s) 406
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HincII GTYRAC 1 cut(s) 211
HindII GTYRAC 1 cut(s) 211
HpaII CCGG 5 cut(s) 110, 195, 282, 331, 360
HphI GGTGA 4 cut(s) 43, 118, 271, 349
Hpy166II GTNNAC 1 cut(s) 211
Hpy188III TCNNGA 2 cut(s) 169, 245
Hpy8I GTNNAC 1 cut(s) 211
Hpy99I CGWCG 3 cut(s) 212, 296, 440
HpyAV CCTTC 1 cut(s) 346
HpyCH4V TGCA 2 cut(s) 24, 228
HpyF10VI GCNNNNNNNGC 4 cut(s) 195, 225, 450, 459
HpyF3I CTNAG 3 cut(s) 309, 426, 474
Hsp92II CATG 1 cut(s) 406
HspAI GCGC 1 cut(s) 222
Kzo9I GATC 3 cut(s) 288, 312, 354
LweI GCATC 2 cut(s) 33, 330
MaeIII GTNAC 1 cut(s) 232
MalI GATC 3 cut(s) 290, 314, 356
MbiI CCGCTC 1 cut(s) 375
MboI GATC 3 cut(s) 288, 312, 354
MboII GAAGA 4 cut(s) 7, 283, 331, 462
MfeI CAATTG 1 cut(s) 144
MhlI GDGCHC 3 cut(s) 77, 126, 446
MlsI TGGCCA 1 cut(s) 260
MluCI AATT 3 cut(s) 93, 144, 363
MluNI TGGCCA 1 cut(s) 260
Mox20I TGGCCA 1 cut(s) 260
MscI TGGCCA 1 cut(s) 260
Msp20I TGGCCA 1 cut(s) 260
MspI CCGG 5 cut(s) 110, 195, 282, 331, 360
MspR9I CCNGG 2 cut(s) 257, 332
MunI CAATTG 1 cut(s) 144
MvaI CCWGG 1 cut(s) 257
MvnI CGCG 1 cut(s) 224
MwoI GCNNNNNNNGC 4 cut(s) 195, 225, 450, 459
NciI CCSGG 1 cut(s) 332
NdeII GATC 3 cut(s) 288, 312, 354
NlaIII CATG 1 cut(s) 406
NmeAIII GCCGAG 1 cut(s) 195
PcsI WCGNNNNNNNCGW 1 cut(s) 324
PkrI GCNGC 3 cut(s) 221, 377, 461
Psp124BI GAGCTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 255
PspEI GGTNACC 1 cut(s) 232
PspGI CCWGG 1 cut(s) 255
PspPI GGNCC 3 cut(s) 112, 333, 457
RsaI GTAC 1 cut(s) 419
RsaNI GTAC 1 cut(s) 418
SacI GAGCTC 1 cut(s) 126
SalI GTCGAC 1 cut(s) 209
SatI GCNGC 3 cut(s) 220, 376, 460
Sau3AI GATC 3 cut(s) 288, 312, 354
Sau96I GGNCC 3 cut(s) 112, 333, 457
ScrFI CCNGG 2 cut(s) 257, 332
SduI GDGCHC 3 cut(s) 77, 126, 446
SetI ASST 7 cut(s) 56, 126, 216, 234, 258, 266, 399
SfaNI GCATC 2 cut(s) 33, 330
SgrAI CRCCGGYG 1 cut(s) 194
Sse9I AATT 3 cut(s) 93, 144, 363
SsiI CCGC 7 cut(s) 46, 162, 187, 219, 375, 411, 460
SstI GAGCTC 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 255, 330
TaqI TCGA 7 cut(s) 210, 269, 294, 353, 371, 393, 435
TaqII GACCGA 1 cut(s) 357
TasI AATT 3 cut(s) 93, 144, 363
TauI GCSGC 3 cut(s) 222, 378, 462
TscAI CASTG 2 cut(s) 70, 350
TspDTI ATGAA 1 cut(s) 193
TspRI CASTG 2 cut(s) 70, 350
XapI RAATTY 1 cut(s) 363
XmiI GTMKAC 1 cut(s) 210
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.