MD02G1056100.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0017193
Physical Location & Seq
Forward (+)
4546178 .. 4549130
2953 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1056100.v1.1.491

Sequence Viewer

Length: 477 bp
ATGGCAAAATCTCTCGTCGTCTTCTCTCTCCTCCTCCTCCTCCTCCTCCTCCTCCTCCCCATCTCCCCCCTAACCGCTCACCCGAACACCCCGAACCCAAAACCGAGCCCATCTCCGGCACACACTGACCTCACCAACTACGGCTTCCCAATCGGACTCCTCCCCACCGCCGTCAAGAACTACACCATCAACCGCACCACCGGCGACTTCATCGTCGACCTCGGCAGCACGTGCAAGGTCACCCTCCCTTCCGACAACGTGGCCACCTACTCGAAGAAAATCACCGGAAAGATCGTCGTGGGCAAAATCGCCAAGATCAACGGTATCAGCGTCCGGGCCTTCTTCCGGTGGCAGCCGATCACCGCAATCCGGTCGAGCGGCGACAACTTGGTGTTCGAGATCGGCACGGTCTCCGTCAAGTACCCCACTAAGAACTTCGAAGAGAGCCCCGCTTGCGAGGGCCGCCACTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

159

Amino Acids

17.05

Weight (kDa)

9.61

Isoelectric Point (pI)

35.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 43 - 147 2.1e-27 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19590 AT5G19590
fragaria_vesca FvH4_1g05270
malus_domestica MD02G1056100.v1.1 MD15G1188900.v1.1
prunus_persica Prupe.7G227700_v2.0.a1 Prupe.7G227700_v2.0.a1
pyrus_communis pycom02g04510
rosa_chinensis RchiOBHm_Chr2g0090991
rosa_laevigata RLG00000016164
rosa_multiflora Rmu_sc0000792.1_g000022 Rmu_sc0003305.1_g000003
rosa_wichuraiana Rw2G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 212
AccBSI CCGCTC 2 cut(s) 77, 378
AccI GTMKAC 1 cut(s) 216
AciI CCGC 7 cut(s) 75, 168, 193, 363, 378, 450, 463
AcoI YGGCCR 1 cut(s) 261
AcvI CACGTG 1 cut(s) 231
AfaI GTAC 1 cut(s) 422
AfiI CCNNNNNNNGG 3 cut(s) 115, 345, 369
AjuI GAANNNNNNNTTGG 2 cut(s) 128, 160
Alw26I GTCTC 1 cut(s) 415
AoxI GGCC 3 cut(s) 261, 336, 460
ApeKI GCWGC 2 cut(s) 225, 352
AspS9I GGNCC 2 cut(s) 336, 460
AsuC2I CCSGG 1 cut(s) 335
AsuHPI GGTGA 5 cut(s) 71, 124, 232, 274, 352
AsuII TTCGAA 1 cut(s) 438
BalI TGGCCA 1 cut(s) 263
BanII GRGCYC 2 cut(s) 110, 449
BbrPI CACGTG 1 cut(s) 231
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 2 cut(s) 237, 364
BccI CCATC 3 cut(s) 68, 118, 194
BceAI ACGGC 2 cut(s) 155, 157
BcgI CGANNNNNNTGC 2 cut(s) 354, 388
BcnI CCSGG 1 cut(s) 335
BcoDI GTCTC 1 cut(s) 415
BisI GCNGC 4 cut(s) 226, 353, 379, 463
BlsI GCNGC 4 cut(s) 227, 354, 380, 464
Bme1390I CCNGG 1 cut(s) 335
BmgT120I GGNCC 2 cut(s) 336, 460
BmrFI CCNGG 1 cut(s) 335
BpiI GAAGAC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 97, 129
Bpu14I TTCGAA 1 cut(s) 438
BpuMI CCSGG 1 cut(s) 335
BsaAI YACGTR 1 cut(s) 231
BsaI GGTCTC 1 cut(s) 415
BsaJI CCNNGG 1 cut(s) 220
BsaWI WCCGGW 3 cut(s) 284, 345, 369
Bsc4I CCNNNNNNNGG 3 cut(s) 115, 345, 369
Bse118I RCCGGY 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 220
BseLI CCNNNNNNNGG 3 cut(s) 115, 345, 369
BseXI GCAGC 2 cut(s) 237, 364
Bsh1285I CGRYCG 1 cut(s) 374
BshFI GGCC 3 cut(s) 263, 338, 462
BsiEI CGRYCG 1 cut(s) 374
BsiSI CCGG 6 cut(s) 116, 201, 285, 334, 346, 370
BslI CCNNNNNNNGG 3 cut(s) 115, 345, 369
BsmAI GTCTC 1 cut(s) 415
BsnI GGCC 3 cut(s) 263, 338, 462
Bso31I GGTCTC 1 cut(s) 415
Bsp119I TTCGAA 1 cut(s) 438
Bsp1286I GDGCHC 2 cut(s) 110, 449
Bsp143I GATC 4 cut(s) 291, 315, 357, 399
BspACI CCGC 7 cut(s) 75, 168, 193, 363, 378, 450, 463
BspANI GGCC 3 cut(s) 263, 338, 462
BspT104I TTCGAA 1 cut(s) 438
BspTNI GGTCTC 1 cut(s) 415
BsrBI CCGCTC 2 cut(s) 77, 378
BsrFI RCCGGY 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 200
BssECI CCNNGG 1 cut(s) 220
BssMI GATC 4 cut(s) 291, 315, 357, 399
Bst4CI ACNGT 2 cut(s) 323, 409
Bst6I CTCTTC 1 cut(s) 435
BstAPI GCANNNNNTGC 1 cut(s) 231
BstBAI YACGTR 1 cut(s) 231
BstBI TTCGAA 1 cut(s) 438
BstC8I GCNNGC 1 cut(s) 454
BstDEI CTNAG 2 cut(s) 429, 474
BstEII GGTNACC 1 cut(s) 238
BstKTI GATC 4 cut(s) 294, 318, 360, 402
BstMAI GTCTC 1 cut(s) 415
BstMBI GATC 4 cut(s) 291, 315, 357, 399
BstMCI CGRYCG 1 cut(s) 374
BstMWI GCNNNNNNNGC 4 cut(s) 201, 231, 453, 462
BstPI GGTNACC 1 cut(s) 238
BstSCI CCNGG 1 cut(s) 333
BstV1I GCAGC 2 cut(s) 237, 364
BstV2I GAAGAC 1 cut(s) 13
BsuRI GGCC 3 cut(s) 263, 338, 462
BtsIMutI CAGTG 1 cut(s) 123
Cac8I GCNNGC 1 cut(s) 454
Cfr10I RCCGGY 1 cut(s) 200
Cfr13I GGNCC 2 cut(s) 336, 460
CseI GACGC 1 cut(s) 319
Csp6I GTAC 1 cut(s) 421
CviJI RGCY 7 cut(s) 108, 144, 263, 338, 355, 447, 462
CviKI_1 RGCY 7 cut(s) 108, 144, 263, 338, 355, 447, 462
CviQI GTAC 1 cut(s) 421
DdeI CTNAG 2 cut(s) 429, 474
DpnI GATC 4 cut(s) 293, 317, 359, 401
DpnII GATC 4 cut(s) 291, 315, 357, 399
DrdI GACNNNNNNGTC 1 cut(s) 212
DseDI GACNNNNNNGTC 1 cut(s) 212
EaeI YGGCCR 1 cut(s) 261
Eam1104I CTCTTC 1 cut(s) 435
EarI CTCTTC 1 cut(s) 435
Eco24I GRGCYC 2 cut(s) 110, 449
Eco31I GGTCTC 1 cut(s) 415
Eco72I CACGTG 1 cut(s) 231
Eco91I GGTNACC 1 cut(s) 238
EcoO65I GGTNACC 1 cut(s) 238
EcoT38I GRGCYC 2 cut(s) 110, 449
FauI CCCGC 1 cut(s) 457
FblI GTMKAC 1 cut(s) 216
Fnu4HI GCNGC 4 cut(s) 226, 353, 379, 463
FriOI GRGCYC 2 cut(s) 110, 449
Fsp4HI GCNGC 4 cut(s) 226, 353, 379, 463
GluI GCNGC 4 cut(s) 226, 353, 379, 463
HaeIII GGCC 3 cut(s) 263, 338, 462
HapII CCGG 6 cut(s) 116, 201, 285, 334, 346, 370
HgaI GACGC 1 cut(s) 319
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HinfI GANTC 1 cut(s) 156
HpaII CCGG 6 cut(s) 116, 201, 285, 334, 346, 370
HphI GGTGA 5 cut(s) 71, 124, 232, 274, 352
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 2 cut(s) 155, 253
Hpy188III TCNNGA 2 cut(s) 175, 397
Hpy8I GTNNAC 1 cut(s) 217
Hpy99I CGWCG 3 cut(s) 20, 218, 299
HpyAV CCTTC 2 cut(s) 258, 349
HpyCH4III ACNGT 2 cut(s) 323, 409
HpyCH4IV ACGT 2 cut(s) 230, 258
HpyCH4V TGCA 1 cut(s) 234
HpyF10VI GCNNNNNNNGC 4 cut(s) 201, 231, 453, 462
HpyF3I CTNAG 2 cut(s) 429, 474
HpySE526I ACGT 2 cut(s) 230, 258
Kzo9I GATC 4 cut(s) 291, 315, 357, 399
LpnPI CCDG 6 cut(s) 129, 214, 298, 347, 359, 383
Lsp1109I GCAGC 2 cut(s) 237, 364
MaeII ACGT 2 cut(s) 230, 258
MaeIII GTNAC 1 cut(s) 238
MalI GATC 4 cut(s) 293, 317, 359, 401
MbiI CCGCTC 2 cut(s) 77, 378
MboI GATC 4 cut(s) 291, 315, 357, 399
MboII GAAGA 4 cut(s) 13, 286, 334, 452
MhlI GDGCHC 2 cut(s) 110, 449
MlsI TGGCCA 1 cut(s) 263
MluNI TGGCCA 1 cut(s) 263
MlyI GAGTC 1 cut(s) 150
MmeI TCCRAC 1 cut(s) 276
Mox20I TGGCCA 1 cut(s) 263
MscI TGGCCA 1 cut(s) 263
Msp20I TGGCCA 1 cut(s) 263
MspI CCGG 6 cut(s) 116, 201, 285, 334, 346, 370
MspR9I CCNGG 1 cut(s) 335
MwoI GCNNNNNNNGC 4 cut(s) 201, 231, 453, 462
NciI CCSGG 1 cut(s) 335
NdeII GATC 4 cut(s) 291, 315, 357, 399
NmeAIII GCCGAG 1 cut(s) 201
NmuCI GTSAC 1 cut(s) 238
NspV TTCGAA 1 cut(s) 438
PcsI WCGNNNNNNNCGW 1 cut(s) 327
PkrI GCNGC 4 cut(s) 227, 354, 380, 464
PleI GAGTC 1 cut(s) 150
PmaCI CACGTG 1 cut(s) 231
PmlI CACGTG 1 cut(s) 231
PpsI GAGTC 1 cut(s) 150
Ppu21I YACGTR 1 cut(s) 231
PspCI CACGTG 1 cut(s) 231
PspEI GGTNACC 1 cut(s) 238
PspPI GGNCC 2 cut(s) 336, 460
RsaI GTAC 1 cut(s) 422
RsaNI GTAC 1 cut(s) 421
SalI GTCGAC 1 cut(s) 215
SatI GCNGC 4 cut(s) 226, 353, 379, 463
Sau3AI GATC 4 cut(s) 291, 315, 357, 399
Sau96I GGNCC 2 cut(s) 336, 460
SchI GAGTC 1 cut(s) 150
ScrFI CCNGG 1 cut(s) 335
SduI GDGCHC 2 cut(s) 110, 449
SetI ASST 6 cut(s) 132, 222, 233, 240, 261, 269
SfuI TTCGAA 1 cut(s) 438
SgrAI CRCCGGYG 1 cut(s) 200
SsiI CCGC 7 cut(s) 75, 168, 193, 363, 378, 450, 463
StyD4I CCNGG 1 cut(s) 333
TaaI ACNGT 2 cut(s) 323, 409
TaiI ACGT 2 cut(s) 233, 261
TaqI TCGA 5 cut(s) 216, 272, 374, 396, 438
TauI GCSGC 2 cut(s) 381, 465
TscAI CASTG 1 cut(s) 130
TseFI GTSAC 1 cut(s) 238
TseI GCWGC 2 cut(s) 225, 352
Tsp45I GTSAC 1 cut(s) 238
TspDTI ATGAA 1 cut(s) 199
TspGWI ACGGA 1 cut(s) 403
TspRI CASTG 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 216
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.