pycom02g04510

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0017193
Physical Location & Seq
Forward (+)
2954952 .. 2955419
468 bp
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UTR
Exon/CDS
Intron
pycom02g04510.1

Sequence Viewer

Length: 468 bp
ATGGCAAAATCTCTCGTCGTCTTCTCTCTCCTCCTCCTCCTCCTCCTCCTCATCTCCCCCCTAACCGCTGACCCGAACACCCCGAACCCAAAACCGAGCCCATCTCCGGCCCACACCGACCTCACCAACTACGGCTTCCCAATCGGCCTCCTCCCCACCGCCGTCAAGAACTACACCATCAACCGCTCCACCGGCGACTTCATCGTCGACCTCGGCGGCGCGTGCAAGGTCACCCTCCCTTCCGACAACGTGGCCACCTACTCGAAGAAAATCACCGGAAAGATCGTCGTGGGCAAAATCACCAAGATCAATGGCATCAGCGTCCGGGCCTTCTTCCGGTGGCAGCCGATCACCGGAATCCGGTCGAGCGGCGACAACTTGGTGTTCGAGATCGGCACGGTCTCCGTCAAGTACCCCACTAAGAACTTCGATGAGAGTCCCGCTTGCGAGGGCCGCCACTCCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

156

Amino Acids

16.63

Weight (kDa)

9.48

Isoelectric Point (pI)

38.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 40 - 143 3.8e-27 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19590 AT5G19590
fragaria_vesca FvH4_1g05270
malus_domestica MD02G1056100.v1.1 MD15G1188900.v1.1
prunus_persica Prupe.7G227700_v2.0.a1 Prupe.7G227700_v2.0.a1
pyrus_communis pycom02g04510
rosa_chinensis RchiOBHm_Chr2g0090991
rosa_laevigata RLG00000016164
rosa_multiflora Rmu_sc0000792.1_g000022 Rmu_sc0003305.1_g000003
rosa_wichuraiana Rw2G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
AccBSI CCGCTC 2 cut(s) 186, 369
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 221
AciI CCGC 7 cut(s) 66, 159, 184, 216, 369, 441, 454
AcoI YGGCCR 1 cut(s) 252
AfaI GTAC 1 cut(s) 413
AfiI CCNNNNNNNGG 4 cut(s) 106, 336, 353, 360
AjuI GAANNNNNNNTTGG 2 cut(s) 119, 151
Alw26I GTCTC 1 cut(s) 406
AoxI GGCC 5 cut(s) 108, 145, 252, 327, 451
ApeKI GCWGC 1 cut(s) 343
AspLEI GCGC 1 cut(s) 221
AspS9I GGNCC 3 cut(s) 109, 327, 451
AsuC2I CCSGG 1 cut(s) 326
AsuHPI GGTGA 5 cut(s) 115, 223, 265, 292, 343
BalI TGGCCA 1 cut(s) 254
BanII GRGCYC 1 cut(s) 101
BbsI GAAGAC 1 cut(s) 13
BbvI GCAGC 1 cut(s) 355
BccI CCATC 2 cut(s) 109, 185
BceAI ACGGC 2 cut(s) 146, 148
BcnI CCSGG 1 cut(s) 326
BcoDI GTCTC 1 cut(s) 406
BisI GCNGC 4 cut(s) 217, 344, 370, 454
BlsI GCNGC 4 cut(s) 218, 345, 371, 455
Bme1390I CCNGG 1 cut(s) 326
BmgT120I GGNCC 3 cut(s) 109, 327, 451
BmrFI CCNGG 1 cut(s) 326
BmsI GCATC 1 cut(s) 324
BpiI GAAGAC 1 cut(s) 13
BplI GAGNNNNNCTC 2 cut(s) 88, 120
BpuMI CCSGG 1 cut(s) 326
BsaI GGTCTC 1 cut(s) 406
BsaJI CCNNGG 1 cut(s) 211
BsaWI WCCGGW 4 cut(s) 275, 336, 353, 360
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 336, 353, 360
Bse118I RCCGGY 1 cut(s) 191
BseDI CCNNGG 1 cut(s) 211
BseLI CCNNNNNNNGG 4 cut(s) 106, 336, 353, 360
BseRI GAGGAG 9 cut(s) 20, 23, 26, 29, 32, 35, 38, 140, 451
BseXI GCAGC 1 cut(s) 355
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 365
BshFI GGCC 5 cut(s) 110, 147, 254, 329, 453
BsiEI CGRYCG 1 cut(s) 365
BsiSI CCGG 7 cut(s) 107, 192, 276, 325, 337, 354, 361
BslFI GGGAC 1 cut(s) 423
BslI CCNNNNNNNGG 4 cut(s) 106, 336, 353, 360
BsmAI GTCTC 1 cut(s) 406
BsmFI GGGAC 1 cut(s) 423
BsnI GGCC 5 cut(s) 110, 147, 254, 329, 453
Bso31I GGTCTC 1 cut(s) 406
Bsp1286I GDGCHC 1 cut(s) 101
Bsp143I GATC 4 cut(s) 282, 306, 348, 390
BspACI CCGC 7 cut(s) 66, 159, 184, 216, 369, 441, 454
BspANI GGCC 5 cut(s) 110, 147, 254, 329, 453
BspFNI CGCG 1 cut(s) 221
BspTNI GGTCTC 1 cut(s) 406
BsrBI CCGCTC 2 cut(s) 186, 369
BsrFI RCCGGY 1 cut(s) 191
BssAI RCCGGY 1 cut(s) 191
BssECI CCNNGG 1 cut(s) 211
BssMI GATC 4 cut(s) 282, 306, 348, 390
Bst4CI ACNGT 1 cut(s) 400
BstC8I GCNNGC 2 cut(s) 223, 445
BstDEI CTNAG 2 cut(s) 420, 465
BstEII GGTNACC 1 cut(s) 229
BstFNI CGCG 1 cut(s) 221
BstHHI GCGC 1 cut(s) 221
BstKTI GATC 4 cut(s) 285, 309, 351, 393
BstMAI GTCTC 1 cut(s) 406
BstMBI GATC 4 cut(s) 282, 306, 348, 390
BstMCI CGRYCG 1 cut(s) 365
BstMWI GCNNNNNNNGC 3 cut(s) 192, 222, 453
BstPI GGTNACC 1 cut(s) 229
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 1 cut(s) 221
BstV1I GCAGC 1 cut(s) 355
BstV2I GAAGAC 1 cut(s) 13
BsuRI GGCC 5 cut(s) 110, 147, 254, 329, 453
Cac8I GCNNGC 2 cut(s) 223, 445
CfoI GCGC 1 cut(s) 221
Cfr10I RCCGGY 1 cut(s) 191
Cfr13I GGNCC 3 cut(s) 109, 327, 451
CseI GACGC 1 cut(s) 310
Csp6I GTAC 1 cut(s) 412
CviJI RGCY 8 cut(s) 99, 110, 135, 147, 254, 329, 346, 453
CviKI_1 RGCY 8 cut(s) 99, 110, 135, 147, 254, 329, 346, 453
CviQI GTAC 1 cut(s) 412
DdeI CTNAG 2 cut(s) 420, 465
DpnI GATC 4 cut(s) 284, 308, 350, 392
DpnII GATC 4 cut(s) 282, 306, 348, 390
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 1 cut(s) 252
Eco24I GRGCYC 1 cut(s) 101
Eco31I GGTCTC 1 cut(s) 406
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoT38I GRGCYC 1 cut(s) 101
FaqI GGGAC 1 cut(s) 423
FauI CCCGC 1 cut(s) 448
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 4 cut(s) 217, 344, 370, 454
FriOI GRGCYC 1 cut(s) 101
Fsp4HI GCNGC 4 cut(s) 217, 344, 370, 454
GlaI GCGC 1 cut(s) 220
GluI GCNGC 4 cut(s) 217, 344, 370, 454
HaeIII GGCC 5 cut(s) 110, 147, 254, 329, 453
HapII CCGG 7 cut(s) 107, 192, 276, 325, 337, 354, 361
HgaI GACGC 1 cut(s) 310
HhaI GCGC 1 cut(s) 221
Hin6I GCGC 1 cut(s) 219
HinP1I GCGC 1 cut(s) 219
HincII GTYRAC 1 cut(s) 208
HindII GTYRAC 1 cut(s) 208
HinfI GANTC 2 cut(s) 357, 436
HpaII CCGG 7 cut(s) 107, 192, 276, 325, 337, 354, 361
HphI GGTGA 5 cut(s) 115, 223, 265, 292, 343
Hpy166II GTNNAC 1 cut(s) 208
Hpy188I TCNGA 1 cut(s) 244
Hpy188III TCNNGA 2 cut(s) 166, 388
Hpy8I GTNNAC 1 cut(s) 208
Hpy99I CGWCG 3 cut(s) 20, 209, 290
HpyAV CCTTC 2 cut(s) 249, 340
HpyCH4III ACNGT 1 cut(s) 400
HpyCH4IV ACGT 1 cut(s) 249
HpyCH4V TGCA 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 3 cut(s) 192, 222, 453
HpyF3I CTNAG 2 cut(s) 420, 465
HpySE526I ACGT 1 cut(s) 249
HspAI GCGC 1 cut(s) 219
Kzo9I GATC 4 cut(s) 282, 306, 348, 390
LmnI GCTCC 1 cut(s) 191
LpnPI CCDG 7 cut(s) 120, 205, 289, 338, 350, 367, 374
Lsp1109I GCAGC 1 cut(s) 355
LweI GCATC 1 cut(s) 324
MaeII ACGT 1 cut(s) 249
MaeIII GTNAC 1 cut(s) 229
MalI GATC 4 cut(s) 284, 308, 350, 392
MbiI CCGCTC 2 cut(s) 186, 369
MboI GATC 4 cut(s) 282, 306, 348, 390
MboII GAAGA 3 cut(s) 13, 277, 325
MhlI GDGCHC 1 cut(s) 101
MlsI TGGCCA 1 cut(s) 254
MluNI TGGCCA 1 cut(s) 254
MlyI GAGTC 1 cut(s) 445
MmeI TCCRAC 1 cut(s) 267
Mox20I TGGCCA 1 cut(s) 254
MscI TGGCCA 1 cut(s) 254
Msp20I TGGCCA 1 cut(s) 254
MspA1I CMGCKG 1 cut(s) 68
MspI CCGG 7 cut(s) 107, 192, 276, 325, 337, 354, 361
MspR9I CCNGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 3 cut(s) 192, 222, 453
NciI CCSGG 1 cut(s) 326
NdeII GATC 4 cut(s) 282, 306, 348, 390
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 1 cut(s) 229
PfeI GAWTC 1 cut(s) 357
PkrI GCNGC 4 cut(s) 218, 345, 371, 455
PleI GAGTC 1 cut(s) 444
PpsI GAGTC 1 cut(s) 444
PspEI GGTNACC 1 cut(s) 229
PspPI GGNCC 3 cut(s) 109, 327, 451
RsaI GTAC 1 cut(s) 413
RsaNI GTAC 1 cut(s) 412
SalI GTCGAC 1 cut(s) 206
SatI GCNGC 4 cut(s) 217, 344, 370, 454
Sau3AI GATC 4 cut(s) 282, 306, 348, 390
Sau96I GGNCC 3 cut(s) 109, 327, 451
SchI GAGTC 1 cut(s) 445
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 1 cut(s) 101
SetI ASST 5 cut(s) 123, 213, 231, 252, 260
SfaNI GCATC 1 cut(s) 324
SgrAI CRCCGGYG 1 cut(s) 191
SsiI CCGC 7 cut(s) 66, 159, 184, 216, 369, 441, 454
StyD4I CCNGG 1 cut(s) 324
TaaI ACNGT 1 cut(s) 400
TaiI ACGT 1 cut(s) 252
TaqI TCGA 5 cut(s) 207, 263, 365, 387, 429
TauI GCSGC 3 cut(s) 219, 372, 456
TfiI GAWTC 1 cut(s) 357
TseFI GTSAC 1 cut(s) 229
TseI GCWGC 1 cut(s) 343
Tsp45I GTSAC 1 cut(s) 229
TspDTI ATGAA 1 cut(s) 190
TspGWI ACGGA 1 cut(s) 394
XmiI GTMKAC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.