RLG00000016164

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0017193
Physical Location & Seq
Forward (+)
4389825 .. 4390301
477 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016164

Sequence Viewer

Length: 477 bp
ATGACGAAATATCTCCTCTTCTTCTTCTTCGCTCTCGTCCTCCTCGTTTCTCTCTCCTCCCAAACCGCCGACCCGAAAACCCAAAACCCGACCCGAACCCCATCGCCGGCCCACACCGAGCTGACCCGCTACGGCTTCCCAATCGGGCTCCTCCCTTCTCCGGTCAAAAACTACACCGTCAACCCCCTCACCGGCGACTTCGTCGTCGACCTCGGCGACGCGTGCAAGATCACGCTCCCGCCGGACAACTACTTGGCCACGTATTCCAAGAAAGTCACCGGAAAAATCAACCACGGCCGGATCGCCAAAATCAACGGCATCAGCGTCCGGGCCTTCTACCAGGGGTGGTCGATCACCGGAATCAGGTCCAGCGGCGAGAATTTGGTGTTTGAGGTCGGCATGGTGACGGCGAGGTACCCCTCTAAGAACTTCAATGATAGTCCGGCTTGCGAGGGCCAACGCCACTCCGCTTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

159

Amino Acids

17.32

Weight (kDa)

9.45

Isoelectric Point (pI)

33.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 38 - 145 7.2e-25 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017193)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G19590 AT5G19590
fragaria_vesca FvH4_1g05270
malus_domestica MD02G1056100.v1.1 MD15G1188900.v1.1
prunus_persica Prupe.7G227700_v2.0.a1 Prupe.7G227700_v2.0.a1
pyrus_communis pycom02g04510
rosa_chinensis RchiOBHm_Chr2g0090991
rosa_laevigata RLG00000016164
rosa_multiflora Rmu_sc0000792.1_g000022 Rmu_sc0003305.1_g000003
rosa_wichuraiana Rw2G005170

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 203
Acc65I GGTACC 1 cut(s) 414
AccB1I GGYRCC 1 cut(s) 414
AccI GTMKAC 1 cut(s) 207
AccII CGCG 1 cut(s) 221
AciI CCGC 5 cut(s) 66, 127, 239, 372, 468
AclWI GGATC 1 cut(s) 308
AcoI YGGCCR 2 cut(s) 255, 295
AcsI RAATTY 1 cut(s) 379
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 4 cut(s) 106, 160, 191, 363
AflIII ACRYGT 1 cut(s) 219
AgsI TTSAA 1 cut(s) 433
AjnI CCWGG 1 cut(s) 339
AluBI AGCT 1 cut(s) 121
AluI AGCT 1 cut(s) 121
AlwI GGATC 1 cut(s) 308
AoxI GGCC 5 cut(s) 108, 255, 295, 330, 454
ApoI RAATTY 1 cut(s) 379
Asp718I GGTACC 1 cut(s) 414
AspS9I GGNCC 4 cut(s) 109, 330, 366, 454
AsuC2I CCSGG 1 cut(s) 329
AsuHPI GGTGA 4 cut(s) 181, 268, 346, 415
AvaII GGWCC 1 cut(s) 366
BalI TGGCCA 1 cut(s) 257
BanI GGYRCC 1 cut(s) 414
BanII GRGCYC 1 cut(s) 150
BccI CCATC 1 cut(s) 109
BceAI ACGGC 4 cut(s) 148, 310, 331, 423
BciT130I CCWGG 1 cut(s) 341
BcnI CCSGG 1 cut(s) 329
BisI GCNGC 1 cut(s) 373
BlsI GCNGC 1 cut(s) 374
Bme1390I CCNGG 2 cut(s) 329, 341
Bme18I GGWCC 1 cut(s) 366
BmgT120I GGNCC 4 cut(s) 109, 330, 366, 454
BmiI GGNNCC 2 cut(s) 149, 416
BmrFI CCNGG 2 cut(s) 329, 341
BmsI GCATC 1 cut(s) 327
BpuMI CCSGG 1 cut(s) 329
BsaAI YACGTR 1 cut(s) 261
BsaJI CCNNGG 3 cut(s) 211, 292, 340
BsaWI WCCGGW 3 cut(s) 160, 278, 356
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 160, 191, 363
Bse118I RCCGGY 2 cut(s) 106, 191
BseBI CCWGG 1 cut(s) 341
BseDI CCNNGG 3 cut(s) 211, 292, 340
BseLI CCNNNNNNNGG 4 cut(s) 106, 160, 191, 363
BseRI GAGGAG 4 cut(s) 5, 32, 46, 140
BseX3I CGGCCG 1 cut(s) 295
Bsh1236I CGCG 1 cut(s) 221
Bsh1285I CGRYCG 1 cut(s) 298
BshFI GGCC 5 cut(s) 110, 257, 297, 332, 456
BshNI GGYRCC 1 cut(s) 414
BsiEI CGRYCG 1 cut(s) 298
BsiSI CCGG 9 cut(s) 107, 161, 192, 242, 279, 298, 328, 357, 443
BslI CCNNNNNNNGG 4 cut(s) 106, 160, 191, 363
BsnI GGCC 5 cut(s) 110, 257, 297, 332, 456
Bsp1286I GDGCHC 1 cut(s) 150
Bsp143I GATC 3 cut(s) 228, 300, 351
BspACI CCGC 5 cut(s) 66, 127, 239, 372, 468
BspANI GGCC 5 cut(s) 110, 257, 297, 332, 456
BspFNI CGCG 1 cut(s) 221
BspLI GGNNCC 2 cut(s) 149, 416
BspPI GGATC 1 cut(s) 308
BspT107I GGYRCC 1 cut(s) 414
BsrFI RCCGGY 2 cut(s) 106, 191
BssAI RCCGGY 2 cut(s) 106, 191
BssECI CCNNGG 3 cut(s) 211, 292, 340
BssMI GATC 3 cut(s) 228, 300, 351
Bst2UI CCWGG 1 cut(s) 341
Bst4CI ACNGT 1 cut(s) 178
Bst6I CTCTTC 1 cut(s) 23
BstBAI YACGTR 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 108, 223, 448
BstDEI CTNAG 1 cut(s) 423
BstDSI CCRYGG 1 cut(s) 292
BstFNI CGCG 1 cut(s) 221
BstKTI GATC 3 cut(s) 231, 303, 354
BstMBI GATC 3 cut(s) 228, 300, 351
BstMCI CGRYCG 1 cut(s) 298
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstNI CCWGG 1 cut(s) 341
BstSCI CCNGG 2 cut(s) 327, 339
BstUI CGCG 1 cut(s) 221
BstZI CGGCCG 1 cut(s) 295
BsuRI GGCC 5 cut(s) 110, 257, 297, 332, 456
BtgI CCRYGG 1 cut(s) 292
BtgZI GCGATG 1 cut(s) 87
Cac8I GCNNGC 3 cut(s) 108, 223, 448
Cfr10I RCCGGY 2 cut(s) 106, 191
Cfr13I GGNCC 4 cut(s) 109, 330, 366, 454
CseI GACGC 2 cut(s) 227, 313
Csp6I GTAC 1 cut(s) 415
CviAII CATG 1 cut(s) 400
CviJI RGCY 9 cut(s) 110, 121, 135, 148, 257, 297, 332, 446, 456
CviKI_1 RGCY 9 cut(s) 110, 121, 135, 148, 257, 297, 332, 446, 456
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 1 cut(s) 423
DpnI GATC 3 cut(s) 230, 302, 353
DpnII GATC 3 cut(s) 228, 300, 351
DrdI GACNNNNNNGTC 1 cut(s) 203
DseDI GACNNNNNNGTC 1 cut(s) 203
EaeI YGGCCR 2 cut(s) 255, 295
EagI CGGCCG 1 cut(s) 295
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
EclXI CGGCCG 1 cut(s) 295
Eco24I GRGCYC 1 cut(s) 150
Eco47I GGWCC 1 cut(s) 366
Eco52I CGGCCG 1 cut(s) 295
EcoRII CCWGG 1 cut(s) 339
EcoT38I GRGCYC 1 cut(s) 150
FaeI CATG 1 cut(s) 403
FaiI YATR 1 cut(s) 401
FatI CATG 1 cut(s) 399
FauI CCCGC 2 cut(s) 134, 246
FblI GTMKAC 1 cut(s) 207
Fnu4HI GCNGC 1 cut(s) 373
FriOI GRGCYC 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 373
GluI GCNGC 1 cut(s) 373
HaeIII GGCC 5 cut(s) 110, 257, 297, 332, 456
HapII CCGG 9 cut(s) 107, 161, 192, 242, 279, 298, 328, 357, 443
HgaI GACGC 2 cut(s) 227, 313
Hin1II CATG 1 cut(s) 403
HincII GTYRAC 2 cut(s) 181, 208
HindII GTYRAC 2 cut(s) 181, 208
HinfI GANTC 1 cut(s) 360
HpaII CCGG 9 cut(s) 107, 161, 192, 242, 279, 298, 328, 357, 443
HphI GGTGA 4 cut(s) 181, 268, 346, 415
Hpy166II GTNNAC 2 cut(s) 181, 208
Hpy8I GTNNAC 2 cut(s) 181, 208
Hpy99I CGWCG 3 cut(s) 206, 209, 221
HpyAV CCTTC 2 cut(s) 165, 343
HpyCH4III ACNGT 1 cut(s) 178
HpyCH4IV ACGT 1 cut(s) 260
HpyCH4V TGCA 1 cut(s) 225
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 1 cut(s) 423
HpySE526I ACGT 1 cut(s) 260
Hsp92II CATG 1 cut(s) 403
KpnI GGTACC 1 cut(s) 418
KroI GCCGGC 1 cut(s) 106
KroNI GCCGGC 1 cut(s) 108
Kzo9I GATC 3 cut(s) 228, 300, 351
LmnI GCTCC 2 cut(s) 153, 240
LweI GCATC 1 cut(s) 327
MaeII ACGT 1 cut(s) 260
MaeIII GTNAC 2 cut(s) 274, 403
MalI GATC 3 cut(s) 230, 302, 353
MboI GATC 3 cut(s) 228, 300, 351
MboII GAAGA 4 cut(s) 10, 13, 16, 19
MhlI GDGCHC 1 cut(s) 150
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 1 cut(s) 379
MluI ACGCGT 1 cut(s) 219
MluNI TGGCCA 1 cut(s) 257
Mox20I TGGCCA 1 cut(s) 257
MroNI GCCGGC 1 cut(s) 106
MscI TGGCCA 1 cut(s) 257
MseI TTAA 1 cut(s) 475
Msp20I TGGCCA 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 372
MspI CCGG 9 cut(s) 107, 161, 192, 242, 279, 298, 328, 357, 443
MspR9I CCNGG 2 cut(s) 329, 341
MvaI CCWGG 1 cut(s) 341
MvnI CGCG 1 cut(s) 221
MwoI GCNNNNNNNGC 1 cut(s) 222
NaeI GCCGGC 1 cut(s) 108
NciI CCSGG 1 cut(s) 329
NdeII GATC 3 cut(s) 228, 300, 351
NgoMIV GCCGGC 1 cut(s) 106
NlaIII CATG 1 cut(s) 403
NlaIV GGNNCC 2 cut(s) 149, 416
NmeAIII GCCGAG 1 cut(s) 192
NmuCI GTSAC 2 cut(s) 274, 403
PcsI WCGNNNNNNNCGW 3 cut(s) 42, 213, 321
PdiI GCCGGC 1 cut(s) 108
PfeI GAWTC 1 cut(s) 360
PflFI GACNNNGTC 1 cut(s) 200
PkrI GCNGC 1 cut(s) 374
Ppu21I YACGTR 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 339
PspGI CCWGG 1 cut(s) 339
PspN4I GGNNCC 2 cut(s) 149, 416
PspPI GGNCC 4 cut(s) 109, 330, 366, 454
PsyI GACNNNGTC 1 cut(s) 200
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
SalI GTCGAC 1 cut(s) 206
SaqAI TTAA 1 cut(s) 475
SatI GCNGC 1 cut(s) 373
Sau3AI GATC 3 cut(s) 228, 300, 351
Sau96I GGNCC 4 cut(s) 109, 330, 366, 454
ScrFI CCNGG 2 cut(s) 329, 341
SduI GDGCHC 1 cut(s) 150
SetI ASST 6 cut(s) 123, 213, 263, 368, 396, 416
SfaNI GCATC 1 cut(s) 327
SgrAI CRCCGGYG 1 cut(s) 191
SinI GGWCC 1 cut(s) 366
Sse9I AATT 1 cut(s) 379
SsiI CCGC 5 cut(s) 66, 127, 239, 372, 468
StyD4I CCNGG 2 cut(s) 327, 339
TaaI ACNGT 1 cut(s) 178
TaiI ACGT 1 cut(s) 263
TaqI TCGA 2 cut(s) 207, 350
TasI AATT 1 cut(s) 379
TauI GCSGC 1 cut(s) 375
TfiI GAWTC 1 cut(s) 360
Tru1I TTAA 1 cut(s) 475
Tru9I TTAA 1 cut(s) 475
TseFI GTSAC 2 cut(s) 274, 403
Tsp45I GTSAC 2 cut(s) 274, 403
Tth111I GACNNNGTC 1 cut(s) 200
VpaK11BI GGWCC 1 cut(s) 366
XapI RAATTY 1 cut(s) 379
XmiI GTMKAC 1 cut(s) 207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.