FvH4_1g06040

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0016726
Physical Location & Seq
Forward (+)
3222310 .. 3226560
4251 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g06040.t1

Sequence Viewer

Length: 1116 bp
ATGGACGAACCCCAACTCGGCCGGGTCGACTCACTCGACCTCGCCTCCACCAGCTCCTCCGGCCTCTCCTCCATCCTCACCTCCGACGACTCCAGAAGCACCACCAGCAGCTCCGGCGAGTTCTCGAGAACCACCGCCGGAAGCTCCGGCGAGATTCCGGCGCTTCTGGCGGTGCCGGAGGCTAGGCCGAAGTGCGTGGGGAGGAACAATAAGGGAGTGACGTGGGGGTCCACTGCGGTGATTGGGAGGCGGAAGGAGATGGAGGACGCCGTGGCCGTTATTCCCGGGTTTATGGCTTGCACGTGCGATCACGTGGGGGGATGTACCGCTCCCGGTTCGAGAACCTCCTCCGAGATCTCCCCCGTCCATTTCTTCGGTGTCTACGACGGCCACGGCGGCAGTCAGGTGGCTAAATTTTGTGCTGAGCGAATGCATGAAGTCATAGCACAAGAGTGGGATAAGGAGATGGTTGATGGCTGTGAATGGCAAAGGAGGTGGGAGACTACTTTCCTAGTTGGGTTCGAGAGGGCTGATAGTGAGGTCCTGACAGAGGCAGATGCGCCTGAGATGGTTGGATCAACTGCAGTTGTAGTTGTCCTATCTGGTTGCCAGATTATCACATCCAACTGTGGTGACTCTAGGGCGATTCTTTGCCGAGGGACAGAAACAATCCCGTTAACTGTTGATCAGAAGCCCGACAGAGAAGACGAACTCATTAGAATTGAAGGAGAGGGAGGGAGAGTCATTAACTGGAATGGGGCAAGGGTGTTCGGAGTTCTTGCCATGTCAAGAGCTATAGGTGATCGGTATCTAAGGCCTTGGATAATTCCAGTTCCTGAGATTACATTCACAGCGAGGACTGATGAGGATGAGTGTTTGATCTTGGCAAGTGATGGACTGTGGGATGTAATGACTAATGAAGAGGTTGGGGAGGTGGCTAGACATATTTTAAGAAGGCGGCGAAGGTCCATGTTTACAAATACTGAGGAAATTTCTGCAGCACAAGCTGTTGCTGATCATCTTACTGAAATTGCATACGGAAGAAATAGTTCCGACAACATCTCCATCATAGTTGTTGATCTCAAAATGAAAAGGAAACGTCAGCCAAGGCAATGA

Protein Analysis

372

Amino Acids

40.55

Weight (kDa)

5.04

Isoelectric Point (pI)

61.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 78 - 354 6.2e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 226
AccB1I GGYRCC 1 cut(s) 172
AccBSI CCGCTC 1 cut(s) 329
AccI GTMKAC 2 cut(s) 27, 381
AciI CCGC 7 cut(s) 135, 170, 236, 250, 327, 396, 958
AclWI GGATC 1 cut(s) 583
AcoI YGGCCR 3 cut(s) 19, 273, 388
AcsI RAATTY 2 cut(s) 413, 990
AcvI CACGTG 2 cut(s) 303, 313
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 1 cut(s) 325
AfiI CCNNNNNNNGG 2 cut(s) 17, 550
AgsI TTSAA 1 cut(s) 725
AjiI CACGTC 1 cut(s) 222
AleI CACNNNNGTG 2 cut(s) 236, 451
AluBI AGCT 5 cut(s) 54, 111, 144, 794, 1007
AluI AGCT 5 cut(s) 54, 111, 144, 794, 1007
Alw26I GTCTC 1 cut(s) 494
AlwI GGATC 1 cut(s) 583
AlwNI CAGNNNCTG 1 cut(s) 836
Ama87I CYCGRG 2 cut(s) 124, 284
AoxI GGCC 6 cut(s) 19, 61, 185, 273, 388, 815
ApeKI GCWGC 2 cut(s) 108, 998
ApoI RAATTY 2 cut(s) 413, 990
Asp700I GAANNNNTTC 1 cut(s) 1048
AspLEI GCGC 2 cut(s) 163, 562
AspS9I GGNCC 3 cut(s) 228, 541, 966
AsuC2I CCSGG 4 cut(s) 23, 285, 286, 333
AsuHPI GGTGA 4 cut(s) 70, 250, 644, 812
AvaI CYCGRG 2 cut(s) 124, 284
AvaII GGWCC 3 cut(s) 228, 541, 966
BanI GGYRCC 1 cut(s) 172
BbrPI CACGTG 2 cut(s) 303, 313
BbsI GAAGAC 1 cut(s) 711
BbvI GCAGC 2 cut(s) 120, 1010
BccI CCATC 7 cut(s) 80, 253, 460, 467, 562, 887, 1073
BceAI ACGGC 4 cut(s) 254, 260, 403, 409
BclI TGATCA 2 cut(s) 685, 1015
BcnI CCSGG 4 cut(s) 23, 285, 286, 333
BcoDI GTCTC 1 cut(s) 494
BfaI CTAG 4 cut(s) 183, 512, 639, 939
BfmI CTRYAG 3 cut(s) 582, 795, 996
BfoI RGCGCY 1 cut(s) 164
BglI GCCNNNNNGGC 1 cut(s) 396
BglII AGATCT 1 cut(s) 354
BisI GCNGC 4 cut(s) 109, 397, 959, 999
BlpI GCTNAGC 1 cut(s) 423
BlsI GCNGC 4 cut(s) 110, 398, 960, 1000
Bme1390I CCNGG 4 cut(s) 23, 285, 286, 333
Bme18I GGWCC 3 cut(s) 228, 541, 966
BmeT110I CYCGRG 2 cut(s) 124, 284
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 3 cut(s) 228, 541, 966
BmiI GGNNCC 2 cut(s) 174, 229
BmrFI CCNGG 4 cut(s) 23, 285, 286, 333
BmsI GCATC 1 cut(s) 547
BpiI GAAGAC 1 cut(s) 711
BpmI CTGGAG 1 cut(s) 76
Bpu1102I GCTNAGC 1 cut(s) 423
BpuMI CCSGG 4 cut(s) 23, 285, 286, 333
BsaAI YACGTR 2 cut(s) 303, 313
BsaBI GATNNNNATC 1 cut(s) 807
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 6 cut(s) 270, 284, 391, 655, 818, 1106
BsaXI ACNNNNNCTCC 6 cut(s) 29, 59, 726, 756, 1046, 1076
Bsc4I CCNNNNNNNGG 2 cut(s) 17, 550
Bse1I ACTGG 2 cut(s) 755, 830
Bse8I GATNNNNATC 1 cut(s) 807
BseDI CCNNGG 6 cut(s) 270, 284, 391, 655, 818, 1106
BseGI GGATG 5 cut(s) 72, 326, 620, 874, 910
BseJI GATNNNNATC 1 cut(s) 807
BseLI CCNNNNNNNGG 2 cut(s) 17, 550
BseMII CTCAG 4 cut(s) 414, 555, 828, 975
BseNI ACTGG 2 cut(s) 755, 830
BseRI GAGGAG 3 cut(s) 46, 58, 337
BseX3I CGGCCG 1 cut(s) 19
BseXI GCAGC 2 cut(s) 120, 1010
Bsh1285I CGRYCG 1 cut(s) 22
BshFI GGCC 6 cut(s) 21, 63, 187, 275, 390, 817
BshNI GGYRCC 1 cut(s) 172
BsiEI CGRYCG 1 cut(s) 22
BsiHKCI CYCGRG 2 cut(s) 124, 284
BsiSI CCGG 9 cut(s) 22, 60, 114, 138, 147, 158, 176, 285, 333
BslFI GGGAC 1 cut(s) 673
BslI CCNNNNNNNGG 2 cut(s) 17, 550
BsmAI GTCTC 1 cut(s) 494
BsmFI GGGAC 1 cut(s) 673
BsmI GAATGC 1 cut(s) 435
BsnI GGCC 6 cut(s) 21, 63, 187, 275, 390, 817
BsoBI CYCGRG 2 cut(s) 124, 284
Bsp143I GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
Bsp1720I GCTNAGC 1 cut(s) 423
BspACI CCGC 7 cut(s) 135, 170, 236, 250, 327, 396, 958
BspANI GGCC 6 cut(s) 21, 63, 187, 275, 390, 817
BspCNI CTCAG 4 cut(s) 415, 556, 829, 976
BspLI GGNNCC 2 cut(s) 174, 229
BspMAI CTGCAG 2 cut(s) 586, 1000
BspPI GGATC 1 cut(s) 583
BspT107I GGYRCC 1 cut(s) 172
BsrBI CCGCTC 1 cut(s) 329
BsrI ACTGG 2 cut(s) 755, 830
BssECI CCNNGG 6 cut(s) 270, 284, 391, 655, 818, 1106
BssMI GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 818, 1106
Bst4CI ACNGT 3 cut(s) 629, 682, 900
Bst6I CTCTTC 1 cut(s) 915
BstACI GRCGYC 1 cut(s) 267
BstBAI YACGTR 2 cut(s) 303, 313
BstC8I GCNNGC 1 cut(s) 298
BstDEI CTNAG 5 cut(s) 423, 564, 812, 837, 984
BstDSI CCRYGG 2 cut(s) 270, 391
BstENI CCTNNNNNAGG 1 cut(s) 548
BstF5I GGATG 5 cut(s) 72, 326, 620, 874, 910
BstH2I RGCGCY 1 cut(s) 164
BstHHI GCGC 2 cut(s) 163, 562
BstKTI GATC 8 cut(s) 310, 357, 578, 688, 805, 882, 1018, 1081
BstMAI GTCTC 1 cut(s) 494
BstMBI GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
BstMCI CGRYCG 1 cut(s) 22
BstMWI GCNNNNNNNGC 6 cut(s) 60, 105, 114, 167, 396, 1004
BstSCI CCNGG 4 cut(s) 21, 283, 284, 331
BstSFI CTRYAG 3 cut(s) 582, 795, 996
BstV1I GCAGC 2 cut(s) 120, 1010
BstV2I GAAGAC 1 cut(s) 711
BstX2I RGATCY 1 cut(s) 354
BstYI RGATCY 1 cut(s) 354
BstZI CGGCCG 1 cut(s) 19
BsuRI GGCC 6 cut(s) 21, 63, 187, 275, 390, 817
BtgI CCRYGG 2 cut(s) 270, 391
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 5 cut(s) 72, 326, 620, 874, 910
BtsI GCAGTG 1 cut(s) 231
BtsIMutI CAGTG 1 cut(s) 231
Cac8I GCNNGC 1 cut(s) 298
CaiI CAGNNNCTG 1 cut(s) 836
CfoI GCGC 2 cut(s) 163, 562
Cfr13I GGNCC 3 cut(s) 228, 541, 966
Cfr9I CCCGGG 1 cut(s) 284
CseI GACGC 1 cut(s) 275
Csp6I GTAC 1 cut(s) 324
CspCI CAANNNNNGTGG 2 cut(s) 476, 511
CviAII CATG 3 cut(s) 434, 784, 970
CviQI GTAC 1 cut(s) 324
DdeI CTNAG 5 cut(s) 423, 564, 812, 837, 984
DpnI GATC 8 cut(s) 309, 356, 577, 687, 804, 881, 1017, 1080
DpnII GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
DrdI GACNNNNNNGTC 1 cut(s) 226
DseDI GACNNNNNNGTC 1 cut(s) 226
EaeI YGGCCR 3 cut(s) 19, 273, 388
EagI CGGCCG 1 cut(s) 19
Eam1104I CTCTTC 1 cut(s) 915
EarI CTCTTC 1 cut(s) 915
EciI GGCGGA 1 cut(s) 265
EclXI CGGCCG 1 cut(s) 19
Eco130I CCWWGG 2 cut(s) 818, 1106
Eco147I AGGCCT 1 cut(s) 817
Eco47I GGWCC 3 cut(s) 228, 541, 966
Eco52I CGGCCG 1 cut(s) 19
Eco72I CACGTG 2 cut(s) 303, 313
Eco88I CYCGRG 2 cut(s) 124, 284
EcoNI CCTNNNNNAGG 1 cut(s) 548
EcoO109I RGGNCCY 1 cut(s) 541
EcoT14I CCWWGG 2 cut(s) 818, 1106
EcoT22I ATGCAT 1 cut(s) 435
ErhI CCWWGG 2 cut(s) 818, 1106
FaeI CATG 3 cut(s) 437, 787, 973
FaiI YATR 9 cut(s) 293, 435, 443, 785, 797, 945, 971, 1036, 1070
FaqI GGGAC 1 cut(s) 673
FatI CATG 3 cut(s) 433, 783, 969
FbaI TGATCA 2 cut(s) 685, 1015
FblI GTMKAC 2 cut(s) 27, 381
Fnu4HI GCNGC 4 cut(s) 109, 397, 959, 999
FokI GGATG 5 cut(s) 59, 333, 607, 881, 917
Fsp4HI GCNGC 4 cut(s) 109, 397, 959, 999
FspBI CTAG 4 cut(s) 183, 512, 639, 939
GlaI GCGC 2 cut(s) 162, 561
GluI GCNGC 4 cut(s) 109, 397, 959, 999
GsuI CTGGAG 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 164
HaeIII GGCC 6 cut(s) 21, 63, 187, 275, 390, 817
HapII CCGG 9 cut(s) 22, 60, 114, 138, 147, 158, 176, 285, 333
HgaI GACGC 1 cut(s) 275
HhaI GCGC 2 cut(s) 163, 562
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 437, 787, 973
Hin6I GCGC 2 cut(s) 161, 560
HinP1I GCGC 2 cut(s) 161, 560
HincII GTYRAC 2 cut(s) 28, 678
HindII GTYRAC 2 cut(s) 28, 678
HinfI GANTC 6 cut(s) 29, 89, 154, 635, 646, 741
HpaI GTTAAC 1 cut(s) 678
HpaII CCGG 9 cut(s) 22, 60, 114, 138, 147, 158, 176, 285, 333
HphI GGTGA 4 cut(s) 70, 250, 644, 812
Hpy166II GTNNAC 5 cut(s) 28, 231, 382, 678, 975
Hpy188I TCNGA 5 cut(s) 85, 352, 690, 773, 1054
Hpy188III TCNNGA 8 cut(s) 93, 124, 126, 339, 523, 544, 789, 836
Hpy8I GTNNAC 5 cut(s) 28, 231, 382, 678, 975
Hpy99I CGWCG 2 cut(s) 89, 389
HpyAV CCTTC 4 cut(s) 247, 719, 948, 957
HpyCH4III ACNGT 3 cut(s) 629, 682, 900
HpyCH4IV ACGT 4 cut(s) 221, 302, 312, 1099
HpyCH4V TGCA 5 cut(s) 300, 433, 584, 998, 1034
HpyF10VI GCNNNNNNNGC 6 cut(s) 60, 105, 114, 167, 396, 1004
HpyF3I CTNAG 5 cut(s) 423, 564, 812, 837, 984
HpySE526I ACGT 4 cut(s) 221, 302, 312, 1099
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 437, 787, 973
HspAI GCGC 2 cut(s) 161, 560
Ksp22I TGATCA 2 cut(s) 685, 1015
KspAI GTTAAC 1 cut(s) 678
Kzo9I GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
LmnI GCTCC 4 cut(s) 59, 116, 149, 334
Lsp1109I GCAGC 2 cut(s) 120, 1010
LweI GCATC 1 cut(s) 547
MaeI CTAG 4 cut(s) 183, 512, 639, 939
MaeII ACGT 4 cut(s) 221, 302, 312, 1099
MaeIII GTNAC 2 cut(s) 217, 632
MalI GATC 8 cut(s) 309, 356, 577, 687, 804, 881, 1017, 1080
MbiI CCGCTC 1 cut(s) 329
MboI GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
MboII GAAGA 4 cut(s) 364, 716, 932, 1053
MflI RGATCY 1 cut(s) 354
MluCI AATT 5 cut(s) 413, 720, 825, 990, 1029
MlyI GAGTC 4 cut(s) 23, 83, 629, 750
MmeI TCCRAC 4 cut(s) 108, 553, 648, 1077
Mph1103I ATGCAT 1 cut(s) 435
MroXI GAANNNNTTC 1 cut(s) 1048
MseI TTAA 3 cut(s) 677, 747, 950
MslI CAYNNNNRTG 2 cut(s) 236, 451
MspI CCGG 9 cut(s) 22, 60, 114, 138, 147, 158, 176, 285, 333
MspR9I CCNGG 4 cut(s) 23, 285, 286, 333
Mva1269I GAATGC 1 cut(s) 435
MwoI GCNNNNNNNGC 6 cut(s) 60, 105, 114, 167, 396, 1004
NciI CCSGG 4 cut(s) 23, 285, 286, 333
NdeII GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
NlaIII CATG 3 cut(s) 437, 787, 973
NlaIV GGNNCC 2 cut(s) 174, 229
NmeAIII GCCGAG 1 cut(s) 680
NmuCI GTSAC 2 cut(s) 217, 632
NsiI ATGCAT 1 cut(s) 435
OliI CACNNNNGTG 2 cut(s) 236, 451
PaeR7I CTCGAG 1 cut(s) 124
PceI AGGCCT 1 cut(s) 817
PcsI WCGNNNNNNNCGW 4 cut(s) 24, 33, 273, 381
PctI GAATGC 1 cut(s) 435
PdmI GAANNNNTTC 1 cut(s) 1048
PfeI GAWTC 2 cut(s) 154, 646
PkrI GCNGC 4 cut(s) 110, 398, 960, 1000
PleI GAGTC 4 cut(s) 23, 83, 629, 749
PmaCI CACGTG 2 cut(s) 303, 313
PmlI CACGTG 2 cut(s) 303, 313
PpsI GAGTC 4 cut(s) 23, 83, 629, 749
Ppu21I YACGTR 2 cut(s) 303, 313
PpuMI RGGWCCY 1 cut(s) 541
Psp5II RGGWCCY 1 cut(s) 541
PspCI CACGTG 2 cut(s) 303, 313
PspN4I GGNNCC 2 cut(s) 174, 229
PspPI GGNCC 3 cut(s) 228, 541, 966
PspPPI RGGWCCY 1 cut(s) 541
PstI CTGCAG 2 cut(s) 586, 1000
PstNI CAGNNNCTG 1 cut(s) 836
PsuI RGATCY 1 cut(s) 354
RsaI GTAC 1 cut(s) 325
RsaNI GTAC 1 cut(s) 324
RseI CAYNNNNRTG 2 cut(s) 236, 451
SalI GTCGAC 1 cut(s) 26
SaqAI TTAA 3 cut(s) 677, 747, 950
SatI GCNGC 4 cut(s) 109, 397, 959, 999
Sau3AI GATC 8 cut(s) 307, 354, 575, 685, 802, 879, 1015, 1078
Sau96I GGNCC 3 cut(s) 228, 541, 966
SchI GAGTC 4 cut(s) 23, 83, 629, 750
ScrFI CCNGG 4 cut(s) 23, 285, 286, 333
SfaNI GCATC 1 cut(s) 547
SfcI CTRYAG 3 cut(s) 582, 795, 996
Sfr274I CTCGAG 1 cut(s) 124
SinI GGWCC 3 cut(s) 228, 541, 966
SlaI CTCGAG 1 cut(s) 124
SmaI CCCGGG 1 cut(s) 286
SmiMI CAYNNNNRTG 2 cut(s) 236, 451
SmlI CTYRAG 1 cut(s) 124
SmoI CTYRAG 1 cut(s) 124
Sse9I AATT 5 cut(s) 413, 720, 825, 990, 1029
SseBI AGGCCT 1 cut(s) 817
SsiI CCGC 7 cut(s) 135, 170, 236, 250, 327, 396, 958
SspMI CTAG 4 cut(s) 183, 512, 639, 939
StuI AGGCCT 1 cut(s) 817
StyD4I CCNGG 4 cut(s) 21, 283, 284, 331
StyI CCWWGG 2 cut(s) 818, 1106
TaaI ACNGT 3 cut(s) 629, 682, 900
TaiI ACGT 4 cut(s) 224, 305, 315, 1102
TaqI TCGA 5 cut(s) 27, 36, 125, 338, 522
TasI AATT 5 cut(s) 413, 720, 825, 990, 1029
TauI GCSGC 2 cut(s) 399, 961
TfiI GAWTC 2 cut(s) 154, 646
Tru1I TTAA 3 cut(s) 677, 747, 950
Tru9I TTAA 3 cut(s) 677, 747, 950
TscAI CASTG 1 cut(s) 238
TseFI GTSAC 2 cut(s) 217, 632
TseI GCWGC 2 cut(s) 108, 998
Tsp45I GTSAC 2 cut(s) 217, 632
TspDTI ATGAA 3 cut(s) 450, 933, 1103
TspGWI ACGGA 1 cut(s) 1053
TspMI CCCGGG 1 cut(s) 284
TspRI CASTG 1 cut(s) 238
VpaK11BI GGWCC 3 cut(s) 228, 541, 966
XagI CCTNNNNNAGG 1 cut(s) 548
XapI RAATTY 2 cut(s) 413, 990
XhoI CTCGAG 1 cut(s) 124
XmaI CCCGGG 1 cut(s) 284
XmiI GTMKAC 2 cut(s) 27, 381
XmnI GAANNNNTTC 1 cut(s) 1048
XspI CTAG 4 cut(s) 183, 512, 639, 939
Zsp2I ATGCAT 1 cut(s) 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.