Rroxscaffold_2G00149730

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0016726
Physical Location & Seq
Reverse (-)
87231366 .. 87236243
4878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00149730.1

Sequence Viewer

Length: 1581 bp
ATGGAATCCACAGACCCCGATCCCCAACTCGACCGAGTCGACTCACTCACCGCCGACCTCGAACTCGCCTCCGCCAGCTCCACCAGCCTCTCCTCCATACTCACCGCCGACGACTCCAGAAGCCCCACCAGCAGCTCCGGTGAGTTCTCCAGGACCACCACCAGCAGCTCTGGTGAGATTCCGGCGTTTCTGCCCCCTGCCGGGGAGGAGATAAGACCCCCTCCGGCGGCGGTTAGGCCGAAGTGCGTTGGGAGGAACAATAAGGGAGTGACGTGGGGGTTCACTTCCGTGATTGGGAGGAGGAGAGAGATGGAGGACGCGGTGGCTGTTATTCCCGGGTTTATGGCTCGCACGTGCGATCACGTGGGGGGTTGTACGGCTCCCGGTTCAAGAACCTCCTCCGAGATCTCCCCCGTCCATTTCTTCGGCGTCTACGACGGCCATGGCGGCAGTCAGGTACTGCAATTTTCTTGGAGGATAGAGGAAAAGAATAAAAATATTAGAGTAACAGTAGCAGTAGTGTGGAATTATGAATATTTAAGTCGGAGGAATGATTGCTTCCCAAGAGAGGACGACACGTGGTCAGCAAAGAGTGGCCGTCATTTATGGATTTTATGCTTTATGGCGGGTTTCGTTTTCGGCCTGGACACGTGTCGCGTTGAGAGTGGATGCCAACGTCACGGGTATGCGTTGTCTTGTCGTCACAGGCGCATCATTTATGGCGCACTCTCGTGGGGCGCTAGTGTCTTGTTGTCTGATGAGGACGAATCAGGATCAAGGAGTTCCATAATTGTTGGAAAGAAAAATCGGAGTGGGGCCTTCCTTGTTTGTGCTCTTGTGGTTCCTTTTAAATTTATAAAGGAAGGGACGGTGGCTAAATATTGTGCTGAGCGAATGCATGAAGTCATAGCACAAGAGTGGGATCGGGAGACGGTTGATGGCTGTGAATGGCAAAGGAGGTGGGAGACTACTTTCTTAGTTGGGTTTGAGAAGGCTGACAGTGAGGTCCTCACAGAGGCAGTTGCGCCTGAGATGGTTGGATCAACTGCAGTTGTAGTTGTCCTATCTGGTTGTCAGATCATCACATCGAACTGTGGTGACTCTAGGGCAGTTCTTTGCCGAGGGACAGAAACAATCCCATTAACTGTTGATCAGAAGCCTGATAGAGAAGACGAACTCACGAGAATTGAAGGAGAGGGAGGGAAAGTCATAAACTGGAATGGCGCAAGGGTGCTTGGAGTTCTTGCCATGTCGAGAGCTATAGGTGATCGATATCTAAGGCCTTGGATAATTCCAGTTCCTGAGATTACATTCACAGCGAGGACTGATGAGGATGAGTGTTTGATTTTGGCCAGTGATGGACTGTGGGATGTAATGACTAATGAAGAGGTTGGGGAGGTGGCTAGACATATTTTAAGAAGGCGGCGAAGGTCCATGTCTTCTAGTACTGATGATATTTCTGCAGCACAAGCTGCTGCTGATCATCTGACCGAAATTGCATATGGAAGAAATAGTTCCGACAACATATCCATTATAGTTGTTGATCTCAAAACAAAAAGAAAACGTCAGCCAAGGCAATGA

Protein Analysis

526

Amino Acids

58.03

Weight (kDa)

5.67

Isoelectric Point (pI)

59.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 291 - 509 9.2e-51 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
AasI GACNNNNNNGTC 1 cut(s) 1004
AccI GTMKAC 2 cut(s) 39, 432
AccII CGCG 2 cut(s) 320, 657
AciI CCGC 9 cut(s) 51, 72, 105, 227, 230, 320, 447, 626, 1423
AclWI GGATC 4 cut(s) 14, 781, 930, 1048
AcoI YGGCCR 3 cut(s) 439, 595, 1350
AcsI RAATTY 1 cut(s) 851
AcvI CACGTG 4 cut(s) 354, 364, 579, 651
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 3 cut(s) 376, 459, 1447
AfiI CCNNNNNNNGG 7 cut(s) 200, 201, 202, 226, 294, 568, 1015
AflIII ACRYGT 3 cut(s) 576, 648, 650
AgsI TTSAA 2 cut(s) 390, 1190
AhdI GACNNNNNGTC 1 cut(s) 580
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 2 cut(s) 149, 642
AleI CACNNNNGTG 3 cut(s) 287, 730, 916
AluBI AGCT 5 cut(s) 78, 135, 168, 1259, 1472
AluI AGCT 5 cut(s) 78, 135, 168, 1259, 1472
Alw21I GWGCWC 1 cut(s) 835
Alw26I GTCTC 2 cut(s) 923, 959
AlwI GGATC 4 cut(s) 14, 781, 930, 1048
AlwNI CAGNNNCTG 2 cut(s) 460, 1301
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 7 cut(s) 236, 439, 595, 640, 816, 1280, 1350
ApeKI GCWGC 5 cut(s) 132, 165, 1463, 1472, 1475
ApoI RAATTY 1 cut(s) 851
Asp700I GAANNNNTTC 1 cut(s) 1513
AspLEI GCGC 5 cut(s) 711, 725, 740, 1027, 1226
AspS9I GGNCC 4 cut(s) 153, 816, 1006, 1431
AsuC2I CCSGG 4 cut(s) 202, 336, 337, 384
AsuHPI GGTGA 6 cut(s) 40, 94, 152, 185, 1109, 1277
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 3 cut(s) 153, 1006, 1431
BalI TGGCCA 1 cut(s) 1352
BauI CACGAG 2 cut(s) 730, 1180
BbrPI CACGTG 4 cut(s) 354, 364, 579, 651
BbsI GAAGAC 2 cut(s) 1176, 1431
Bbv12I GWGCWC 1 cut(s) 835
BbvI GCAGC 5 cut(s) 144, 177, 1459, 1462, 1475
BccI CCATC 4 cut(s) 304, 932, 1027, 1352
BceAI ACGGC 3 cut(s) 393, 454, 582
BciT130I CCWGG 2 cut(s) 151, 644
BclI TGATCA 2 cut(s) 1150, 1480
BcnI CCSGG 4 cut(s) 202, 336, 337, 384
BcoDI GTCTC 2 cut(s) 923, 959
BfaI CTAG 4 cut(s) 741, 1104, 1404, 1443
BfmI CTRYAG 3 cut(s) 1047, 1260, 1461
BfoI RGCGCY 1 cut(s) 741
BglI GCCNNNNNGGC 1 cut(s) 447
BglII AGATCT 1 cut(s) 405
BisI GCNGC 8 cut(s) 133, 166, 228, 448, 1424, 1464, 1473, 1476
BlpI GCTNAGC 1 cut(s) 888
BlsI GCNGC 8 cut(s) 134, 167, 229, 449, 1425, 1465, 1474, 1477
BmcAI AGTACT 1 cut(s) 1447
Bme1390I CCNGG 6 cut(s) 151, 202, 336, 337, 384, 644
Bme18I GGWCC 3 cut(s) 153, 1006, 1431
BmeRI GACNNNNNGTC 1 cut(s) 580
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 4 cut(s) 153, 816, 1006, 1431
BmiI GGNNCC 3 cut(s) 381, 817, 843
BmrFI CCNGG 6 cut(s) 151, 202, 336, 337, 384, 644
BmsI GCATC 2 cut(s) 659, 720
BoxI GACNNNNGTC 1 cut(s) 651
BpiI GAAGAC 2 cut(s) 1176, 1431
BpmI CTGGAG 2 cut(s) 100, 133
Bpu1102I GCTNAGC 1 cut(s) 888
BpuMI CCSGG 4 cut(s) 202, 336, 337, 384
Bsa29I ATCGAT 1 cut(s) 1270
BsaAI YACGTR 4 cut(s) 354, 364, 579, 651
BsaBI GATNNNNATC 1 cut(s) 1272
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 6 cut(s) 201, 335, 442, 1120, 1283, 1571
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 1191, 1221
Bsc4I CCNNNNNNNGG 7 cut(s) 200, 201, 202, 226, 294, 568, 1015
Bse1I ACTGG 3 cut(s) 1220, 1295, 1353
Bse8I GATNNNNATC 1 cut(s) 1272
BseBI CCWGG 2 cut(s) 151, 644
BseCI ATCGAT 1 cut(s) 1270
BseDI CCNNGG 6 cut(s) 201, 335, 442, 1120, 1283, 1571
BseGI GGATG 3 cut(s) 674, 1339, 1375
BseJI GATNNNNATC 1 cut(s) 1272
BseLI CCNNNNNNNGG 7 cut(s) 200, 201, 202, 226, 294, 568, 1015
BseMII CTCAG 3 cut(s) 879, 1020, 1293
BseNI ACTGG 3 cut(s) 1220, 1295, 1353
BseRI GAGGAG 5 cut(s) 82, 221, 313, 316, 388
BseXI GCAGC 5 cut(s) 144, 177, 1459, 1462, 1475
Bsh1236I CGCG 2 cut(s) 320, 657
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 7 cut(s) 238, 441, 597, 642, 818, 1282, 1352
BshVI ATCGAT 1 cut(s) 1270
BsiEI CGRYCG 1 cut(s) 34
BsiHKAI GWGCWC 1 cut(s) 835
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 6 cut(s) 138, 182, 201, 224, 336, 384
BslFI GGGAC 2 cut(s) 880, 1138
BslI CCNNNNNNNGG 7 cut(s) 200, 201, 202, 226, 294, 568, 1015
BsmAI GTCTC 2 cut(s) 923, 959
BsmBI CGTCTC 1 cut(s) 923
BsmFI GGGAC 2 cut(s) 880, 1138
BsmI GAATGC 1 cut(s) 900
BsnI GGCC 7 cut(s) 238, 441, 597, 642, 818, 1282, 1352
BsoBI CYCGRG 1 cut(s) 335
Bsp1286I GDGCHC 1 cut(s) 835
Bsp1720I GCTNAGC 1 cut(s) 888
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 9 cut(s) 51, 72, 105, 227, 230, 320, 447, 626, 1423
BspANI GGCC 7 cut(s) 238, 441, 597, 642, 818, 1282, 1352
BspCNI CTCAG 3 cut(s) 880, 1021, 1294
BspDI ATCGAT 1 cut(s) 1270
BspFNI CGCG 2 cut(s) 320, 657
BspLI GGNNCC 3 cut(s) 381, 817, 843
BspMAI CTGCAG 2 cut(s) 1051, 1465
BspPI GGATC 4 cut(s) 14, 781, 930, 1048
BsrI ACTGG 3 cut(s) 1220, 1295, 1353
BssECI CCNNGG 6 cut(s) 201, 335, 442, 1120, 1283, 1571
BssNI GRCGYC 1 cut(s) 429
BssSI CACGAG 2 cut(s) 730, 1180
BssT1I CCWWGG 3 cut(s) 442, 1283, 1571
Bst2BI CACGAG 2 cut(s) 730, 1180
Bst2UI CCWGG 2 cut(s) 151, 644
Bst4CI ACNGT 7 cut(s) 511, 871, 934, 1001, 1094, 1147, 1365
Bst6I CTCTTC 1 cut(s) 1380
BstACI GRCGYC 1 cut(s) 429
BstAPI GCANNNNNTGC 1 cut(s) 1472
BstBAI YACGTR 4 cut(s) 354, 364, 579, 651
BstC8I GCNNGC 2 cut(s) 76, 349
BstDEI CTNAG 5 cut(s) 888, 976, 1029, 1277, 1302
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 1013
BstF5I GGATG 3 cut(s) 674, 1339, 1375
BstFNI CGCG 2 cut(s) 320, 657
BstH2I RGCGCY 1 cut(s) 741
BstHHI GCGC 5 cut(s) 711, 725, 740, 1027, 1226
BstMAI GTCTC 2 cut(s) 923, 959
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 84, 129, 447, 1469, 1472
BstNI CCWGG 2 cut(s) 151, 644
BstPAI GACNNNNGTC 1 cut(s) 651
BstSCI CCNGG 6 cut(s) 149, 200, 334, 335, 382, 642
BstSFI CTRYAG 3 cut(s) 1047, 1260, 1461
BstUI CGCG 2 cut(s) 320, 657
BstV1I GCAGC 5 cut(s) 144, 177, 1459, 1462, 1475
BstV2I GAAGAC 2 cut(s) 1176, 1431
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 1270
BsuRI GGCC 7 cut(s) 238, 441, 597, 642, 818, 1282, 1352
BsuTUI ATCGAT 1 cut(s) 1270
BtgI CCRYGG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 3 cut(s) 674, 1339, 1375
BtsIMutI CAGTG 2 cut(s) 1006, 1360
Cac8I GCNNGC 2 cut(s) 76, 349
CaiI CAGNNNCTG 2 cut(s) 460, 1301
CfoI GCGC 5 cut(s) 711, 725, 740, 1027, 1226
Cfr13I GGNCC 4 cut(s) 153, 816, 1006, 1431
Cfr9I CCCGGG 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 1270
CseI GACGC 2 cut(s) 326, 418
Csp6I GTAC 3 cut(s) 375, 458, 1446
CspCI CAANNNNNGTGG 2 cut(s) 941, 976
CviAII CATG 4 cut(s) 443, 899, 1249, 1435
CviQI GTAC 3 cut(s) 375, 458, 1446
DdeI CTNAG 5 cut(s) 888, 976, 1029, 1277, 1302
DraI TTTAAA 1 cut(s) 850
DrdI GACNNNNNNGTC 1 cut(s) 1004
DriI GACNNNNNGTC 1 cut(s) 580
DseDI GACNNNNNNGTC 1 cut(s) 1004
EaeI YGGCCR 3 cut(s) 439, 595, 1350
Eam1104I CTCTTC 1 cut(s) 1380
Eam1105I GACNNNNNGTC 1 cut(s) 580
EarI CTCTTC 1 cut(s) 1380
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 3 cut(s) 442, 1283, 1571
Eco147I AGGCCT 1 cut(s) 1282
Eco32I GATATC 1 cut(s) 1274
Eco47I GGWCC 3 cut(s) 153, 1006, 1431
Eco72I CACGTG 4 cut(s) 354, 364, 579, 651
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 1013
EcoO109I RGGNCCY 2 cut(s) 816, 1006
EcoRII CCWGG 2 cut(s) 149, 642
EcoRV GATATC 1 cut(s) 1274
EcoT14I CCWWGG 3 cut(s) 442, 1283, 1571
EcoT22I ATGCAT 1 cut(s) 900
ErhI CCWWGG 3 cut(s) 442, 1283, 1571
Esp3I CGTCTC 1 cut(s) 923
FaeI CATG 4 cut(s) 446, 902, 1252, 1438
FaqI GGGAC 2 cut(s) 880, 1138
FatI CATG 4 cut(s) 442, 898, 1248, 1434
FauI CCCGC 1 cut(s) 619
FauNDI CATATG 1 cut(s) 1501
FbaI TGATCA 2 cut(s) 1150, 1480
FblI GTMKAC 2 cut(s) 39, 432
Fnu4HI GCNGC 8 cut(s) 133, 166, 228, 448, 1424, 1464, 1473, 1476
FokI GGATG 3 cut(s) 681, 1346, 1382
Fsp4HI GCNGC 8 cut(s) 133, 166, 228, 448, 1424, 1464, 1473, 1476
FspBI CTAG 4 cut(s) 741, 1104, 1404, 1443
GlaI GCGC 5 cut(s) 710, 724, 739, 1026, 1225
GluI GCNGC 8 cut(s) 133, 166, 228, 448, 1424, 1464, 1473, 1476
GsuI CTGGAG 2 cut(s) 100, 133
HaeII RGCGCY 1 cut(s) 741
HaeIII GGCC 7 cut(s) 238, 441, 597, 642, 818, 1282, 1352
HapII CCGG 6 cut(s) 138, 182, 201, 224, 336, 384
HgaI GACGC 2 cut(s) 326, 418
HhaI GCGC 5 cut(s) 711, 725, 740, 1027, 1226
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 4 cut(s) 446, 902, 1252, 1438
Hin6I GCGC 5 cut(s) 709, 723, 738, 1025, 1224
HinP1I GCGC 5 cut(s) 709, 723, 738, 1025, 1224
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 7 cut(s) 5, 36, 41, 113, 178, 767, 1100
HpaII CCGG 6 cut(s) 138, 182, 201, 224, 336, 384
HphI GGTGA 6 cut(s) 40, 94, 152, 185, 1109, 1277
Hpy166II GTNNAC 3 cut(s) 40, 282, 433
Hpy188I TCNGA 8 cut(s) 403, 546, 757, 810, 1077, 1155, 1488, 1519
Hpy188III TCNNGA 7 cut(s) 117, 390, 771, 926, 1180, 1254, 1301
Hpy8I GTNNAC 3 cut(s) 40, 282, 433
Hpy99I CGWCG 2 cut(s) 113, 440
HpyAV CCTTC 6 cut(s) 829, 857, 985, 1184, 1413, 1422
HpyCH4III ACNGT 7 cut(s) 511, 871, 934, 1001, 1094, 1147, 1365
HpyCH4IV ACGT 7 cut(s) 272, 353, 363, 578, 650, 676, 1564
HpyCH4V TGCA 5 cut(s) 463, 898, 1049, 1463, 1499
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 129, 447, 1469, 1472
HpyF3I CTNAG 5 cut(s) 888, 976, 1029, 1277, 1302
HpySE526I ACGT 7 cut(s) 272, 353, 363, 578, 650, 676, 1564
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 4 cut(s) 446, 902, 1252, 1438
HspAI GCGC 5 cut(s) 709, 723, 738, 1025, 1224
Ksp22I TGATCA 2 cut(s) 1150, 1480
LmnI GCTCC 3 cut(s) 83, 140, 385
Lsp1109I GCAGC 5 cut(s) 144, 177, 1459, 1462, 1475
LweI GCATC 2 cut(s) 659, 720
MaeI CTAG 4 cut(s) 741, 1104, 1404, 1443
MaeII ACGT 7 cut(s) 272, 353, 363, 578, 650, 676, 1564
MaeIII GTNAC 5 cut(s) 268, 505, 677, 701, 1097
MboII GAAGA 5 cut(s) 415, 1181, 1397, 1431, 1518
MflI RGATCY 1 cut(s) 405
MhlI GDGCHC 1 cut(s) 835
MlsI TGGCCA 1 cut(s) 1352
MluCI AATT 7 cut(s) 464, 526, 789, 851, 1185, 1290, 1494
MluNI TGGCCA 1 cut(s) 1352
MlyI GAGTC 4 cut(s) 35, 45, 107, 1094
MmeI TCCRAC 4 cut(s) 524, 775, 1018, 1542
Mox20I TGGCCA 1 cut(s) 1352
Mph1103I ATGCAT 1 cut(s) 900
MroXI GAANNNNTTC 1 cut(s) 1513
MscI TGGCCA 1 cut(s) 1352
MseI TTAA 4 cut(s) 539, 849, 1142, 1415
MslI CAYNNNNRTG 4 cut(s) 287, 684, 730, 916
Msp20I TGGCCA 1 cut(s) 1352
MspI CCGG 6 cut(s) 138, 182, 201, 224, 336, 384
MspR9I CCNGG 6 cut(s) 151, 202, 336, 337, 384, 644
Mva1269I GAATGC 1 cut(s) 900
MvaI CCWGG 2 cut(s) 151, 644
MvnI CGCG 2 cut(s) 320, 657
MwoI GCNNNNNNNGC 5 cut(s) 84, 129, 447, 1469, 1472
NciI CCSGG 4 cut(s) 202, 336, 337, 384
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 1501
NlaIII CATG 4 cut(s) 446, 902, 1252, 1438
NlaIV GGNNCC 3 cut(s) 381, 817, 843
NmeAIII GCCGAG 1 cut(s) 1145
NmuCI GTSAC 4 cut(s) 268, 677, 701, 1097
NsiI ATGCAT 1 cut(s) 900
OliI CACNNNNGTG 3 cut(s) 287, 730, 916
PceI AGGCCT 1 cut(s) 1282
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 432
PctI GAATGC 1 cut(s) 900
PdmI GAANNNNTTC 1 cut(s) 1513
PfeI GAWTC 3 cut(s) 5, 178, 767
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 8 cut(s) 134, 167, 229, 449, 1425, 1465, 1474, 1477
PleI GAGTC 4 cut(s) 35, 44, 107, 1094
PmaCI CACGTG 4 cut(s) 354, 364, 579, 651
PmlI CACGTG 4 cut(s) 354, 364, 579, 651
PpsI GAGTC 4 cut(s) 35, 44, 107, 1094
Ppu21I YACGTR 4 cut(s) 354, 364, 579, 651
PpuMI RGGWCCY 1 cut(s) 1006
PshAI GACNNNNGTC 1 cut(s) 651
PsiI TTATAA 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 1006
Psp6I CCWGG 2 cut(s) 149, 642
PspCI CACGTG 4 cut(s) 354, 364, 579, 651
PspGI CCWGG 2 cut(s) 149, 642
PspN4I GGNNCC 3 cut(s) 381, 817, 843
PspPI GGNCC 4 cut(s) 153, 816, 1006, 1431
PspPPI RGGWCCY 1 cut(s) 1006
PstI CTGCAG 2 cut(s) 1051, 1465
PstNI CAGNNNCTG 2 cut(s) 460, 1301
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 3 cut(s) 376, 459, 1447
RsaNI GTAC 3 cut(s) 375, 458, 1446
RseI CAYNNNNRTG 4 cut(s) 287, 684, 730, 916
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 4 cut(s) 539, 849, 1142, 1415
SatI GCNGC 8 cut(s) 133, 166, 228, 448, 1424, 1464, 1473, 1476
Sau96I GGNCC 4 cut(s) 153, 816, 1006, 1431
ScaI AGTACT 1 cut(s) 1447
SchI GAGTC 4 cut(s) 35, 45, 107, 1094
ScrFI CCNGG 6 cut(s) 151, 202, 336, 337, 384, 644
SduI GDGCHC 1 cut(s) 835
SfaNI GCATC 2 cut(s) 659, 720
SfcI CTRYAG 3 cut(s) 1047, 1260, 1461
SinI GGWCC 3 cut(s) 153, 1006, 1431
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 4 cut(s) 287, 684, 730, 916
Sse9I AATT 7 cut(s) 464, 526, 789, 851, 1185, 1290, 1494
SseBI AGGCCT 1 cut(s) 1282
SsiI CCGC 9 cut(s) 51, 72, 105, 227, 230, 320, 447, 626, 1423
SspI AATATT 3 cut(s) 499, 536, 881
SspMI CTAG 4 cut(s) 741, 1104, 1404, 1443
StuI AGGCCT 1 cut(s) 1282
StyD4I CCNGG 6 cut(s) 149, 200, 334, 335, 382, 642
StyI CCWWGG 3 cut(s) 442, 1283, 1571
TaaI ACNGT 7 cut(s) 511, 871, 934, 1001, 1094, 1147, 1365
TaiI ACGT 7 cut(s) 275, 356, 366, 581, 653, 679, 1567
TaqI TCGA 6 cut(s) 30, 39, 60, 1088, 1253, 1270
TaqII GACCGA 2 cut(s) 48, 1505
TasI AATT 7 cut(s) 464, 526, 789, 851, 1185, 1290, 1494
TatI WGTACW 1 cut(s) 1445
TauI GCSGC 3 cut(s) 230, 450, 1426
TfiI GAWTC 3 cut(s) 5, 178, 767
Tru1I TTAA 4 cut(s) 539, 849, 1142, 1415
Tru9I TTAA 4 cut(s) 539, 849, 1142, 1415
TscAI CASTG 2 cut(s) 1006, 1360
TseFI GTSAC 4 cut(s) 268, 677, 701, 1097
TseI GCWGC 5 cut(s) 132, 165, 1463, 1472, 1475
Tsp45I GTSAC 4 cut(s) 268, 677, 701, 1097
TspDTI ATGAA 3 cut(s) 546, 915, 1398
TspGWI ACGGA 1 cut(s) 277
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 2 cut(s) 1006, 1360
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 153, 1006, 1431
XagI CCTNNNNNAGG 1 cut(s) 1013
XapI RAATTY 1 cut(s) 851
XmaI CCCGGG 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 39, 432
XmnI GAANNNNTTC 1 cut(s) 1513
XspI CTAG 4 cut(s) 741, 1104, 1404, 1443
ZrmI AGTACT 1 cut(s) 1447
Zsp2I ATGCAT 1 cut(s) 900
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.