RLG00000016252

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0016726
Physical Location & Seq
Forward (+)
5110395 .. 5114449
4055 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000016252

Sequence Viewer

Length: 1164 bp
ATGGAATCCACAGACCCCGATCCCCAACTCGACCGAGTCGACTCACTCACCGCCGACCTCGAACTCGCCTCCGCCAGCTCCACCAGCCTCTCCTCCATACTCACCGCCGACGACTCCAGAAGCCCCACCAGCAGCTCCGGTGAGTTCTCCAGGACCACCACCAGCAGCTCCGGCGAGATTCCGGCGTTTCTGCCCCCTGCCGGGGAGGAGATAAGACCCCCGGCGGCGGTTAGGCCGAAGTGCGTTGGGAGGAACAATAAGGGAGTGACGTGGGGGTTCACTTCGGTGATTGGGAGGAGGAGAGAGATGGAGGACGCGGTGGCTGTTATTCCCGGGTTTATGACTCGCACGTGCGATCACGTGGGGGGTTGTACTGCTCCCGGTTCAAGAACCTCCTCCGAGATCTCCCCCGTCCATTTCTTCGGCGTCTACGACGGCCATGGCGGCAGTCAGGTGGCTAAATATTGTGCTGAGCGAATGCATGAAGTCATAGCACAAGAGTGGGATCGGGAGACGGTTGATGGCTGTGAATGGCAAAGGAGGTGGGAGACTACTTTCTTAGTTGGGTTTGAGAAGGCTGACAGTGAGGTCCTCACAGAGGCAGTTGCGCCTGAGATGGTTGGATCAACTGCAGTTGTAGTTGTCCTATCTGGTTGTCAGATCATCACATCGAACTGTGGTGACTCTAGGGCAGTTCTTTGCCGAGGGACAGAAACAATCCCATTAACTGTTGATCAGAAGCCTGATAGAGAAGACGAACTCACGAGAATTGAAGGAGAGGGAGGGAAAGTCATAAACTGGAATGGCGCAAGGGTGCTTGGAGTTCTTGCCATGTCAAGAGCTATAGGTGATCGATATCTAAGGCCTTGGATAATTCCAGTTCCTGAGATTACATTCACAGCGAGGACTGATGAGGATGAGTGTTTGATTTTGGCAAGTGATGGACTGTGGGATGTAATGACTAATGAAGAGGTTGGGGAGGTGGCTAGACATATTTTAAGAAGGCGGCGAAGGTCCATGTCTTCTAGTACTGATGATATTTCTGCAGCACAAGCTGTTGCTGATCATCTGACCGAAATTGCATATGGAAGAAATAGTTCCGACAACATATCCATTATAGTTGTTGATCTCAAAACGAAAAGAAAACGTCAGCCAAGGCAATGA

Protein Analysis

388

Amino Acids

42.23

Weight (kDa)

5.0

Isoelectric Point (pI)

61.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 96 - 370 5.6e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 587
AccI GTMKAC 2 cut(s) 39, 429
AccII CGCG 1 cut(s) 317
AciI CCGC 8 cut(s) 51, 72, 105, 224, 227, 317, 444, 1006
AclWI GGATC 3 cut(s) 14, 513, 631
AcoI YGGCCR 1 cut(s) 436
AcvI CACGTG 2 cut(s) 351, 361
AcyI GRCGYC 1 cut(s) 426
AfaI GTAC 2 cut(s) 373, 1030
AfiI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 598
AgsI TTSAA 2 cut(s) 387, 773
AjiI CACGTC 1 cut(s) 270
AjnI CCWGG 1 cut(s) 149
AleI CACNNNNGTG 2 cut(s) 284, 499
AluBI AGCT 5 cut(s) 78, 135, 168, 842, 1055
AluI AGCT 5 cut(s) 78, 135, 168, 842, 1055
Alw26I GTCTC 2 cut(s) 506, 542
AlwI GGATC 3 cut(s) 14, 513, 631
AlwNI CAGNNNCTG 1 cut(s) 884
Ama87I CYCGRG 1 cut(s) 332
AoxI GGCC 3 cut(s) 233, 436, 863
ApeKI GCWGC 3 cut(s) 132, 165, 1046
Asp700I GAANNNNTTC 1 cut(s) 1096
AspLEI GCGC 2 cut(s) 610, 809
AspS9I GGNCC 3 cut(s) 153, 589, 1014
AsuC2I CCSGG 5 cut(s) 202, 221, 333, 334, 381
AsuHPI GGTGA 6 cut(s) 40, 94, 152, 298, 692, 860
AvaI CYCGRG 1 cut(s) 332
AvaII GGWCC 3 cut(s) 153, 589, 1014
BauI CACGAG 1 cut(s) 763
BbrPI CACGTG 2 cut(s) 351, 361
BbsI GAAGAC 2 cut(s) 759, 1014
BbvI GCAGC 3 cut(s) 144, 177, 1058
BccI CCATC 4 cut(s) 301, 515, 610, 935
BceAI ACGGC 1 cut(s) 451
BciT130I CCWGG 1 cut(s) 151
BclI TGATCA 2 cut(s) 733, 1063
BcnI CCSGG 5 cut(s) 202, 221, 333, 334, 381
BcoDI GTCTC 2 cut(s) 506, 542
BfaI CTAG 3 cut(s) 687, 987, 1026
BfmI CTRYAG 3 cut(s) 630, 843, 1044
BglI GCCNNNNNGGC 1 cut(s) 444
BglII AGATCT 1 cut(s) 402
BisI GCNGC 6 cut(s) 133, 166, 225, 445, 1007, 1047
BlpI GCTNAGC 1 cut(s) 471
BlsI GCNGC 6 cut(s) 134, 167, 226, 446, 1008, 1048
BmcAI AGTACT 1 cut(s) 1030
Bme1390I CCNGG 6 cut(s) 151, 202, 221, 333, 334, 381
Bme18I GGWCC 3 cut(s) 153, 589, 1014
BmeT110I CYCGRG 1 cut(s) 332
BmgBI CACGTC 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 153, 589, 1014
BmrFI CCNGG 6 cut(s) 151, 202, 221, 333, 334, 381
BpiI GAAGAC 2 cut(s) 759, 1014
BpmI CTGGAG 2 cut(s) 100, 133
Bpu1102I GCTNAGC 1 cut(s) 471
BpuMI CCSGG 5 cut(s) 202, 221, 333, 334, 381
Bsa29I ATCGAT 1 cut(s) 853
BsaAI YACGTR 2 cut(s) 351, 361
BsaBI GATNNNNATC 1 cut(s) 855
BsaHI GRCGYC 1 cut(s) 426
BsaJI CCNNGG 7 cut(s) 201, 219, 332, 439, 703, 866, 1154
BsaWI WCCGGW 1 cut(s) 137
BsaXI ACNNNNNCTCC 2 cut(s) 774, 804
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 598
Bse1I ACTGG 2 cut(s) 803, 878
Bse8I GATNNNNATC 1 cut(s) 855
BseBI CCWGG 1 cut(s) 151
BseCI ATCGAT 1 cut(s) 853
BseDI CCNNGG 7 cut(s) 201, 219, 332, 439, 703, 866, 1154
BseGI GGATG 2 cut(s) 922, 958
BseJI GATNNNNATC 1 cut(s) 855
BseLI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 598
BseMII CTCAG 3 cut(s) 462, 603, 876
BseNI ACTGG 2 cut(s) 803, 878
BseRI GAGGAG 5 cut(s) 82, 221, 310, 313, 385
BseXI GCAGC 3 cut(s) 144, 177, 1058
Bsh1236I CGCG 1 cut(s) 317
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 3 cut(s) 235, 438, 865
BshVI ATCGAT 1 cut(s) 853
BsiEI CGRYCG 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 332
BsiSI CCGG 7 cut(s) 138, 171, 182, 201, 221, 333, 381
BslFI GGGAC 1 cut(s) 721
BslI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 598
BsmAI GTCTC 2 cut(s) 506, 542
BsmBI CGTCTC 1 cut(s) 506
BsmFI GGGAC 1 cut(s) 721
BsmI GAATGC 1 cut(s) 483
BsnI GGCC 3 cut(s) 235, 438, 865
BsoBI CYCGRG 1 cut(s) 332
Bsp1720I GCTNAGC 1 cut(s) 471
Bsp19I CCATGG 1 cut(s) 439
BspACI CCGC 8 cut(s) 51, 72, 105, 224, 227, 317, 444, 1006
BspANI GGCC 3 cut(s) 235, 438, 865
BspCNI CTCAG 3 cut(s) 463, 604, 877
BspDI ATCGAT 1 cut(s) 853
BspFNI CGCG 1 cut(s) 317
BspMAI CTGCAG 2 cut(s) 634, 1048
BspPI GGATC 3 cut(s) 14, 513, 631
BsrI ACTGG 2 cut(s) 803, 878
BssECI CCNNGG 7 cut(s) 201, 219, 332, 439, 703, 866, 1154
BssNI GRCGYC 1 cut(s) 426
BssSI CACGAG 1 cut(s) 763
BssT1I CCWWGG 3 cut(s) 439, 866, 1154
Bst2BI CACGAG 1 cut(s) 763
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 5 cut(s) 517, 584, 677, 730, 948
Bst6I CTCTTC 1 cut(s) 963
BstACI GRCGYC 1 cut(s) 426
BstBAI YACGTR 2 cut(s) 351, 361
BstC8I GCNNGC 1 cut(s) 76
BstDEI CTNAG 5 cut(s) 471, 559, 612, 860, 885
BstDSI CCRYGG 1 cut(s) 439
BstENI CCTNNNNNAGG 1 cut(s) 596
BstF5I GGATG 2 cut(s) 922, 958
BstFNI CGCG 1 cut(s) 317
BstHHI GCGC 2 cut(s) 610, 809
BstMAI GTCTC 2 cut(s) 506, 542
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 84, 129, 171, 444, 1052
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 6 cut(s) 149, 200, 219, 331, 332, 379
BstSFI CTRYAG 3 cut(s) 630, 843, 1044
BstUI CGCG 1 cut(s) 317
BstV1I GCAGC 3 cut(s) 144, 177, 1058
BstV2I GAAGAC 2 cut(s) 759, 1014
BstX2I RGATCY 1 cut(s) 402
BstYI RGATCY 1 cut(s) 402
Bsu15I ATCGAT 1 cut(s) 853
BsuRI GGCC 3 cut(s) 235, 438, 865
BsuTUI ATCGAT 1 cut(s) 853
BtgI CCRYGG 1 cut(s) 439
BtrI CACGTC 1 cut(s) 270
BtsCI GGATG 2 cut(s) 922, 958
BtsIMutI CAGTG 1 cut(s) 589
Cac8I GCNNGC 1 cut(s) 76
CaiI CAGNNNCTG 1 cut(s) 884
CfoI GCGC 2 cut(s) 610, 809
Cfr13I GGNCC 3 cut(s) 153, 589, 1014
Cfr9I CCCGGG 1 cut(s) 332
ClaI ATCGAT 1 cut(s) 853
CseI GACGC 2 cut(s) 323, 415
Csp6I GTAC 2 cut(s) 372, 1029
CspCI CAANNNNNGTGG 2 cut(s) 524, 559
CviAII CATG 4 cut(s) 440, 482, 832, 1018
CviQI GTAC 2 cut(s) 372, 1029
DdeI CTNAG 5 cut(s) 471, 559, 612, 860, 885
DrdI GACNNNNNNGTC 1 cut(s) 587
DseDI GACNNNNNNGTC 1 cut(s) 587
EaeI YGGCCR 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 963
EarI CTCTTC 1 cut(s) 963
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 3 cut(s) 439, 866, 1154
Eco147I AGGCCT 1 cut(s) 865
Eco32I GATATC 1 cut(s) 857
Eco47I GGWCC 3 cut(s) 153, 589, 1014
Eco72I CACGTG 2 cut(s) 351, 361
Eco88I CYCGRG 1 cut(s) 332
EcoNI CCTNNNNNAGG 1 cut(s) 596
EcoO109I RGGNCCY 1 cut(s) 589
EcoRII CCWGG 1 cut(s) 149
EcoRV GATATC 1 cut(s) 857
EcoT14I CCWWGG 3 cut(s) 439, 866, 1154
EcoT22I ATGCAT 1 cut(s) 483
ErhI CCWWGG 3 cut(s) 439, 866, 1154
Esp3I CGTCTC 1 cut(s) 506
FaeI CATG 4 cut(s) 443, 485, 835, 1021
FaqI GGGAC 1 cut(s) 721
FatI CATG 4 cut(s) 439, 481, 831, 1017
FauNDI CATATG 1 cut(s) 1084
FbaI TGATCA 2 cut(s) 733, 1063
FblI GTMKAC 2 cut(s) 39, 429
Fnu4HI GCNGC 6 cut(s) 133, 166, 225, 445, 1007, 1047
FokI GGATG 2 cut(s) 929, 965
Fsp4HI GCNGC 6 cut(s) 133, 166, 225, 445, 1007, 1047
FspBI CTAG 3 cut(s) 687, 987, 1026
GlaI GCGC 2 cut(s) 609, 808
GluI GCNGC 6 cut(s) 133, 166, 225, 445, 1007, 1047
GsuI CTGGAG 2 cut(s) 100, 133
HaeIII GGCC 3 cut(s) 235, 438, 865
HapII CCGG 7 cut(s) 138, 171, 182, 201, 221, 333, 381
HgaI GACGC 2 cut(s) 323, 415
HhaI GCGC 2 cut(s) 610, 809
Hin1I GRCGYC 1 cut(s) 426
Hin1II CATG 4 cut(s) 443, 485, 835, 1021
Hin6I GCGC 2 cut(s) 608, 807
HinP1I GCGC 2 cut(s) 608, 807
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 7 cut(s) 5, 36, 41, 113, 178, 343, 683
HpaII CCGG 7 cut(s) 138, 171, 182, 201, 221, 333, 381
HphI GGTGA 6 cut(s) 40, 94, 152, 298, 692, 860
Hpy166II GTNNAC 3 cut(s) 40, 279, 430
Hpy188I TCNGA 5 cut(s) 400, 660, 738, 1071, 1102
Hpy188III TCNNGA 6 cut(s) 117, 387, 509, 763, 837, 884
Hpy8I GTNNAC 3 cut(s) 40, 279, 430
Hpy99I CGWCG 2 cut(s) 113, 437
HpyAV CCTTC 4 cut(s) 568, 767, 996, 1005
HpyCH4III ACNGT 5 cut(s) 517, 584, 677, 730, 948
HpyCH4IV ACGT 4 cut(s) 269, 350, 360, 1147
HpyCH4V TGCA 4 cut(s) 481, 632, 1046, 1082
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 129, 171, 444, 1052
HpyF3I CTNAG 5 cut(s) 471, 559, 612, 860, 885
HpySE526I ACGT 4 cut(s) 269, 350, 360, 1147
Hsp92I GRCGYC 1 cut(s) 426
Hsp92II CATG 4 cut(s) 443, 485, 835, 1021
HspAI GCGC 2 cut(s) 608, 807
Ksp22I TGATCA 2 cut(s) 733, 1063
LmnI GCTCC 4 cut(s) 83, 140, 173, 382
Lsp1109I GCAGC 3 cut(s) 144, 177, 1058
MaeI CTAG 3 cut(s) 687, 987, 1026
MaeII ACGT 4 cut(s) 269, 350, 360, 1147
MaeIII GTNAC 2 cut(s) 265, 680
MboII GAAGA 5 cut(s) 412, 764, 980, 1014, 1101
MflI RGATCY 1 cut(s) 402
MluCI AATT 3 cut(s) 768, 873, 1077
MlyI GAGTC 5 cut(s) 35, 45, 107, 337, 677
MmeI TCCRAC 2 cut(s) 601, 1125
Mph1103I ATGCAT 1 cut(s) 483
MroXI GAANNNNTTC 1 cut(s) 1096
MseI TTAA 2 cut(s) 725, 998
MslI CAYNNNNRTG 2 cut(s) 284, 499
MspI CCGG 7 cut(s) 138, 171, 182, 201, 221, 333, 381
MspR9I CCNGG 6 cut(s) 151, 202, 221, 333, 334, 381
Mva1269I GAATGC 1 cut(s) 483
MvaI CCWGG 1 cut(s) 151
MvnI CGCG 1 cut(s) 317
MwoI GCNNNNNNNGC 5 cut(s) 84, 129, 171, 444, 1052
NciI CCSGG 5 cut(s) 202, 221, 333, 334, 381
NcoI CCATGG 1 cut(s) 439
NdeI CATATG 1 cut(s) 1084
NlaIII CATG 4 cut(s) 443, 485, 835, 1021
NmeAIII GCCGAG 1 cut(s) 728
NmuCI GTSAC 2 cut(s) 265, 680
NsiI ATGCAT 1 cut(s) 483
OliI CACNNNNGTG 2 cut(s) 284, 499
PceI AGGCCT 1 cut(s) 865
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 429
PctI GAATGC 1 cut(s) 483
PdmI GAANNNNTTC 1 cut(s) 1096
PfeI GAWTC 2 cut(s) 5, 178
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 6 cut(s) 134, 167, 226, 446, 1008, 1048
PleI GAGTC 5 cut(s) 35, 44, 107, 337, 677
PmaCI CACGTG 2 cut(s) 351, 361
PmlI CACGTG 2 cut(s) 351, 361
PpsI GAGTC 5 cut(s) 35, 44, 107, 337, 677
Ppu21I YACGTR 2 cut(s) 351, 361
PpuMI RGGWCCY 1 cut(s) 589
Psp5II RGGWCCY 1 cut(s) 589
Psp6I CCWGG 1 cut(s) 149
PspCI CACGTG 2 cut(s) 351, 361
PspGI CCWGG 1 cut(s) 149
PspPI GGNCC 3 cut(s) 153, 589, 1014
PspPPI RGGWCCY 1 cut(s) 589
PstI CTGCAG 2 cut(s) 634, 1048
PstNI CAGNNNCTG 1 cut(s) 884
PsuI RGATCY 1 cut(s) 402
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 2 cut(s) 373, 1030
RsaNI GTAC 2 cut(s) 372, 1029
RseI CAYNNNNRTG 2 cut(s) 284, 499
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 725, 998
SatI GCNGC 6 cut(s) 133, 166, 225, 445, 1007, 1047
Sau96I GGNCC 3 cut(s) 153, 589, 1014
ScaI AGTACT 1 cut(s) 1030
SchI GAGTC 5 cut(s) 35, 45, 107, 337, 677
ScrFI CCNGG 6 cut(s) 151, 202, 221, 333, 334, 381
SfcI CTRYAG 3 cut(s) 630, 843, 1044
SinI GGWCC 3 cut(s) 153, 589, 1014
SmaI CCCGGG 1 cut(s) 334
SmiMI CAYNNNNRTG 2 cut(s) 284, 499
Sse9I AATT 3 cut(s) 768, 873, 1077
SseBI AGGCCT 1 cut(s) 865
SsiI CCGC 8 cut(s) 51, 72, 105, 224, 227, 317, 444, 1006
SspI AATATT 1 cut(s) 464
SspMI CTAG 3 cut(s) 687, 987, 1026
StuI AGGCCT 1 cut(s) 865
StyD4I CCNGG 6 cut(s) 149, 200, 219, 331, 332, 379
StyI CCWWGG 3 cut(s) 439, 866, 1154
TaaI ACNGT 5 cut(s) 517, 584, 677, 730, 948
TaiI ACGT 4 cut(s) 272, 353, 363, 1150
TaqI TCGA 5 cut(s) 30, 39, 60, 671, 853
TaqII GACCGA 2 cut(s) 48, 1088
TasI AATT 3 cut(s) 768, 873, 1077
TatI WGTACW 2 cut(s) 371, 1028
TauI GCSGC 3 cut(s) 227, 447, 1009
TfiI GAWTC 2 cut(s) 5, 178
Tru1I TTAA 2 cut(s) 725, 998
Tru9I TTAA 2 cut(s) 725, 998
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 2 cut(s) 265, 680
TseI GCWGC 3 cut(s) 132, 165, 1046
Tsp45I GTSAC 2 cut(s) 265, 680
TspDTI ATGAA 2 cut(s) 498, 981
TspMI CCCGGG 1 cut(s) 332
TspRI CASTG 1 cut(s) 589
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 153, 589, 1014
XagI CCTNNNNNAGG 1 cut(s) 596
XmaI CCCGGG 1 cut(s) 332
XmiI GTMKAC 2 cut(s) 39, 429
XmnI GAANNNNTTC 1 cut(s) 1096
XspI CTAG 3 cut(s) 687, 987, 1026
ZrmI AGTACT 1 cut(s) 1030
Zsp2I ATGCAT 1 cut(s) 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.