Rh2BG065200

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0016726
Physical Location & Seq
Forward (+)
4983409 .. 4988106
4698 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG065200.1

Sequence Viewer

Length: 876 bp
ATGGAATCCACAGACCCCGATCCCCAACTCGACCGAGTCGACTCACTCGCCGCCGACCTCGAACTCGCCTCCGCCAGCTCCACCAGCCTCTCCTCCATACTCACCGCCGACGACTCCAGAAGCCCCACCAGCAGCTCCGGCGAGTTCTCCAGGACCACCACCAGCAGCTCCGGCGATATTCCGGCGTTTCTGCCCCCTTCCGGGGAGGAGATAAGACCCCCGGCGGCGGTGGTTAGGCCGAAGTGCGTTGGGAGGAACAATAAGGGAGTGACGTGGGGGTTCACTTCGGTGATTGGGAGGAGGAGAGAGATGGAGGACGCGGTGGCTGTTATTCCCGGGTTTATGGCTCGCACGTGCGATCACGTGGGCGGTTGTACGGCTCCCGGTTCGAGAACCTCCTCCGAGATCTCCCCCGTCCATTTCTTCGGCGTCTACGACGGCCATGGCGGCAGTCAGGTGGCTAAATATTGTGCTGAGCGAATGCATGAAGTCATAGCACAAGAGTGGGATCGGGAGACGGTTGATGGCTTTGAATGGCAAAGGAGGTGGGAGACTACTTTCTTAGTTGGGTTTGAGAAGGCTGACAGTGAGGTCCTCACAGAGGCAGTTGCACCTGAGATGGTTGGATCAACTGCAGTTGTAGTTGTCCTATCTGGTTGTCGGATCATCACATCGAACTGTGGTGACTCTAGGGCAGTTCTTTGCCGAGGGACAGAAACAATCCCATTAACTGTTGATCAGAAGCCTGATAGAGAAGACGAACTCAAGAGAATTGAAGGAGAGGGAGGGAAAGTCATAAACTGGAATGGTGCAAGGGTGCTTGGAGTTCTTGCCATGTCGAGAGCTATAGGTTTTCATCTCTATTTCTCTACTTAA

Protein Analysis

291

Amino Acids

31.35

Weight (kDa)

4.96

Isoelectric Point (pI)

50.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 95 - 289 1.1e-39 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 590
AccI GTMKAC 2 cut(s) 39, 432
AccII CGCG 1 cut(s) 320
AciI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 369, 447
AclWI GGATC 4 cut(s) 14, 516, 634, 671
AcoI YGGCCR 1 cut(s) 439
AcvI CACGTG 2 cut(s) 354, 364
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 1 cut(s) 376
AfiI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
AgsI TTSAA 2 cut(s) 533, 776
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 1 cut(s) 149
AleI CACNNNNGTG 2 cut(s) 287, 502
AluBI AGCT 4 cut(s) 78, 135, 168, 845
AluI AGCT 4 cut(s) 78, 135, 168, 845
Alw26I GTCTC 2 cut(s) 509, 545
AlwI GGATC 4 cut(s) 14, 516, 634, 671
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 2 cut(s) 236, 439
ApeKI GCWGC 2 cut(s) 132, 165
AspS9I GGNCC 2 cut(s) 153, 592
AsuC2I CCSGG 5 cut(s) 202, 221, 336, 337, 384
AsuHPI GGTGA 3 cut(s) 94, 301, 695
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 2 cut(s) 153, 592
BbrPI CACGTG 2 cut(s) 354, 364
BbsI GAAGAC 1 cut(s) 762
BbvI GCAGC 2 cut(s) 144, 177
BccI CCATC 3 cut(s) 304, 518, 613
BceAI ACGGC 2 cut(s) 393, 454
BciT130I CCWGG 1 cut(s) 151
BclI TGATCA 1 cut(s) 736
BcnI CCSGG 5 cut(s) 202, 221, 336, 337, 384
BcoDI GTCTC 2 cut(s) 509, 545
BfaI CTAG 1 cut(s) 690
BfmI CTRYAG 2 cut(s) 633, 846
BglI GCCNNNNNGGC 1 cut(s) 447
BglII AGATCT 1 cut(s) 405
BisI GCNGC 5 cut(s) 51, 133, 166, 225, 448
BlpI GCTNAGC 1 cut(s) 474
BlsI GCNGC 5 cut(s) 52, 134, 167, 226, 449
Bme1390I CCNGG 6 cut(s) 151, 202, 221, 336, 337, 384
Bme18I GGWCC 2 cut(s) 153, 592
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 2 cut(s) 153, 592
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 6 cut(s) 151, 202, 221, 336, 337, 384
BpiI GAAGAC 1 cut(s) 762
BpmI CTGGAG 2 cut(s) 100, 133
Bpu1102I GCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 5 cut(s) 202, 221, 336, 337, 384
BsaAI YACGTR 2 cut(s) 354, 364
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 5 cut(s) 201, 219, 335, 442, 706
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
Bse1I ACTGG 1 cut(s) 806
BseBI CCWGG 1 cut(s) 151
BseDI CCNNGG 5 cut(s) 201, 219, 335, 442, 706
BseLI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
BseMII CTCAG 2 cut(s) 465, 606
BseNI ACTGG 1 cut(s) 806
BseRI GAGGAG 5 cut(s) 82, 221, 313, 316, 388
BseXI GCAGC 2 cut(s) 144, 177
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 2 cut(s) 238, 441
BsiEI CGRYCG 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
BsmAI GTCTC 2 cut(s) 509, 545
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 2 cut(s) 238, 441
BsoBI CYCGRG 1 cut(s) 335
Bsp143I GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
Bsp1720I GCTNAGC 1 cut(s) 474
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 369, 447
BspANI GGCC 2 cut(s) 238, 441
BspCNI CTCAG 2 cut(s) 466, 607
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 1 cut(s) 381
BspMAI CTGCAG 1 cut(s) 637
BspPI GGATC 4 cut(s) 14, 516, 634, 671
BsrI ACTGG 1 cut(s) 806
BssECI CCNNGG 5 cut(s) 201, 219, 335, 442, 706
BssMI GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 1 cut(s) 442
Bst2UI CCWGG 1 cut(s) 151
Bst4CI ACNGT 4 cut(s) 520, 587, 680, 733
BstACI GRCGYC 1 cut(s) 429
BstBAI YACGTR 2 cut(s) 354, 364
BstC8I GCNNGC 2 cut(s) 76, 349
BstDEI CTNAG 3 cut(s) 474, 562, 615
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 599
BstFNI CGCG 1 cut(s) 320
BstKTI GATC 7 cut(s) 22, 361, 408, 511, 629, 666, 739
BstMAI GTCTC 2 cut(s) 509, 545
BstMBI GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 5 cut(s) 84, 129, 138, 171, 447
BstNI CCWGG 1 cut(s) 151
BstSCI CCNGG 6 cut(s) 149, 200, 219, 334, 335, 382
BstSFI CTRYAG 2 cut(s) 633, 846
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 2 cut(s) 144, 177
BstV2I GAAGAC 1 cut(s) 762
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
BsuRI GGCC 2 cut(s) 238, 441
BtgI CCRYGG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 2 cut(s) 76, 349
Cfr13I GGNCC 2 cut(s) 153, 592
Cfr9I CCCGGG 1 cut(s) 335
CseI GACGC 2 cut(s) 326, 418
Csp6I GTAC 1 cut(s) 375
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviAII CATG 3 cut(s) 443, 485, 835
CviQI GTAC 1 cut(s) 375
DdeI CTNAG 3 cut(s) 474, 562, 615
DpnI GATC 7 cut(s) 21, 360, 407, 510, 628, 665, 738
DpnII GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 1 cut(s) 439
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 1 cut(s) 442
Eco47I GGWCC 2 cut(s) 153, 592
Eco72I CACGTG 2 cut(s) 354, 364
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 1 cut(s) 149
EcoT14I CCWWGG 1 cut(s) 442
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 1 cut(s) 442
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 3 cut(s) 446, 488, 838
FaiI YATR 8 cut(s) 98, 344, 444, 486, 494, 797, 836, 848
FaqI GGGAC 1 cut(s) 724
FatI CATG 3 cut(s) 442, 484, 834
FbaI TGATCA 1 cut(s) 736
FblI GTMKAC 2 cut(s) 39, 432
Fnu4HI GCNGC 5 cut(s) 51, 133, 166, 225, 448
Fsp4HI GCNGC 5 cut(s) 51, 133, 166, 225, 448
FspBI CTAG 1 cut(s) 690
GluI GCNGC 5 cut(s) 51, 133, 166, 225, 448
GsuI CTGGAG 2 cut(s) 100, 133
HaeIII GGCC 2 cut(s) 238, 441
HapII CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
HgaI GACGC 2 cut(s) 326, 418
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 3 cut(s) 446, 488, 838
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 5 cut(s) 5, 36, 41, 113, 686
HpaII CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
HphI GGTGA 3 cut(s) 94, 301, 695
Hpy166II GTNNAC 3 cut(s) 40, 282, 433
Hpy188I TCNGA 3 cut(s) 403, 663, 741
Hpy188III TCNNGA 5 cut(s) 117, 390, 512, 766, 840
Hpy8I GTNNAC 3 cut(s) 40, 282, 433
Hpy99I CGWCG 2 cut(s) 113, 440
HpyAV CCTTC 3 cut(s) 207, 571, 770
HpyCH4III ACNGT 4 cut(s) 520, 587, 680, 733
HpyCH4IV ACGT 3 cut(s) 272, 353, 363
HpyCH4V TGCA 4 cut(s) 484, 611, 635, 812
HpyF10VI GCNNNNNNNGC 5 cut(s) 84, 129, 138, 171, 447
HpyF3I CTNAG 3 cut(s) 474, 562, 615
HpySE526I ACGT 3 cut(s) 272, 353, 363
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 3 cut(s) 446, 488, 838
Ksp22I TGATCA 1 cut(s) 736
Kzo9I GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
LmnI GCTCC 4 cut(s) 83, 140, 173, 385
Lsp1109I GCAGC 2 cut(s) 144, 177
MaeI CTAG 1 cut(s) 690
MaeII ACGT 3 cut(s) 272, 353, 363
MaeIII GTNAC 2 cut(s) 268, 683
MalI GATC 7 cut(s) 21, 360, 407, 510, 628, 665, 738
MboI GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
MboII GAAGA 2 cut(s) 415, 767
MflI RGATCY 1 cut(s) 405
MluCI AATT 1 cut(s) 771
MlyI GAGTC 4 cut(s) 35, 45, 107, 680
MmeI TCCRAC 2 cut(s) 604, 641
Mph1103I ATGCAT 1 cut(s) 486
MseI TTAA 2 cut(s) 728, 874
MslI CAYNNNNRTG 2 cut(s) 287, 502
MspI CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
MspR9I CCNGG 6 cut(s) 151, 202, 221, 336, 337, 384
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 1 cut(s) 151
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 5 cut(s) 84, 129, 138, 171, 447
NciI CCSGG 5 cut(s) 202, 221, 336, 337, 384
NcoI CCATGG 1 cut(s) 442
NdeII GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
NlaIII CATG 3 cut(s) 446, 488, 838
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 2 cut(s) 268, 683
NsiI ATGCAT 1 cut(s) 486
OliI CACNNNNGTG 2 cut(s) 287, 502
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 432
PctI GAATGC 1 cut(s) 486
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 5 cut(s) 52, 134, 167, 226, 449
PleI GAGTC 4 cut(s) 35, 44, 107, 680
PmaCI CACGTG 2 cut(s) 354, 364
PmlI CACGTG 2 cut(s) 354, 364
PpsI GAGTC 4 cut(s) 35, 44, 107, 680
Ppu21I YACGTR 2 cut(s) 354, 364
PpuMI RGGWCCY 1 cut(s) 592
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 1 cut(s) 149
PspCI CACGTG 2 cut(s) 354, 364
PspGI CCWGG 1 cut(s) 149
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 2 cut(s) 153, 592
PspPPI RGGWCCY 1 cut(s) 592
PstI CTGCAG 1 cut(s) 637
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 1 cut(s) 376
RsaNI GTAC 1 cut(s) 375
RseI CAYNNNNRTG 2 cut(s) 287, 502
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 728, 874
SatI GCNGC 5 cut(s) 51, 133, 166, 225, 448
Sau3AI GATC 7 cut(s) 19, 358, 405, 508, 626, 663, 736
Sau96I GGNCC 2 cut(s) 153, 592
SchI GAGTC 4 cut(s) 35, 45, 107, 680
ScrFI CCNGG 6 cut(s) 151, 202, 221, 336, 337, 384
SfcI CTRYAG 2 cut(s) 633, 846
SinI GGWCC 2 cut(s) 153, 592
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 287, 502
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 1 cut(s) 771
SsiI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 369, 447
SspI AATATT 1 cut(s) 467
SspMI CTAG 1 cut(s) 690
StyD4I CCNGG 6 cut(s) 149, 200, 219, 334, 335, 382
StyI CCWWGG 1 cut(s) 442
TaaI ACNGT 4 cut(s) 520, 587, 680, 733
TaiI ACGT 3 cut(s) 275, 356, 366
TaqI TCGA 6 cut(s) 30, 39, 60, 389, 674, 839
TaqII GACCGA 1 cut(s) 48
TasI AATT 1 cut(s) 771
TauI GCSGC 3 cut(s) 53, 227, 450
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 2 cut(s) 728, 874
Tru9I TTAA 2 cut(s) 728, 874
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 2 cut(s) 268, 683
TseI GCWGC 2 cut(s) 132, 165
Tsp45I GTSAC 2 cut(s) 268, 683
TspDTI ATGAA 2 cut(s) 501, 845
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 1 cut(s) 592
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 2 cut(s) 153, 592
XagI CCTNNNNNAGG 1 cut(s) 599
XmaI CCCGGG 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 39, 432
XspI CTAG 1 cut(s) 690
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.