Rorug02G0021000

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0016726
Physical Location & Seq
Forward (+)
1716638 .. 1718196
1559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0021000.1

Sequence Viewer

Length: 1062 bp
ATGGCTGAATCTTCCACTCCTGCCAAGGTCGAGTTATTAGCAACCAAAACTGTGCAAGAGCAACTCACTGAAGGAGAAGTACTGGTGCCACAGAAATATATTCTCAAAGATGGAATCCCACTTCCAAATGCTTCTGTTGAGTTGATGGATGTTCCGGTTATTGATCTTGGTCTCCTCGCACCTTCCTCATTCAGTATGGAAGAATTTGACAAACTCAGATCAGCTCTTACCAAATGGGGTTGCTTTCAGGTAATAAACCATGGAATGACTCCTGAATTCCTTGACAAAGTCCGTGAAATCACGAAACAATTTTTTGCACTTCCACTGGAAGAGAAGCAGAAATATTTGAGGCCAGCCGACAACATTGAAGGATATGGGAATGACATGGTTTTTTCAGAGCAGCAAACACTTGATTGGACTGATAGACTATACCTTACTGTATATCCACCAGACAAGCGCAAGCTCAAGTTTTGGCCTGAAAATCCCAAATCTTTTAGGGATACTCTAGACCAATATACCAGTAAGTTACAGGTCATAACAGAAACTGTCCTTAAGGGCATGGCGCGGTCATTGAATTTGGAGGAAGATTGCTTTCTGGACAAGTATGGAGAACAAAGGAAAATGGATGCCAGGTTTAACTTCTATCCTCCTTGTTCAAGGCCTGATCGCGTACTAGGTGTCAAGCCACATGCAGATGGAACAATAATCACCCTTCTGTTGCAAGATAAAGAAGTGGAAGGTCTTCAATTTCTGAAAGATGGTCAATGGTTTAGAGCTCCAATTGTTCCTGAGGCACTTCTAATTAATGTTGGTGATCAAGCAGAGATATTGACTAATGGACTGTTCAAGAGCCCAGTGCACAAGGTAGTGACAAATGCAGAAAAGGAGAGGATTTCTTTGGCTACGTTTTGTGTCCCGGATTCAGAGACAGATATCGAACCCTTTGAAGGCCTCATCAATGAGTCAAGGCCAAGGTTGTACAAAAAGTTGAAAAACTACGTTGGCATCTATTTTGAATACTACCAGCAGGGAAGAAGACCAATTGAAGCAGCAATTATATAA

Protein Analysis

353

Amino Acids

40.48

Weight (kDa)

5.18

Isoelectric Point (pI)

44.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DIOX_N PF14226 51 - 161 4.1e-30 non-haem dioxygenase in morphine synthesis N-terminal
2OG-FeII_Oxy PF03171 211 - 306 5e-31 2OG-Fe(II) oxygenase superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 85
AccII CGCG 2 cut(s) 565, 669
AciI CCGC 1 cut(s) 565
AcsI RAATTY 3 cut(s) 203, 275, 574
AcuI CTGAAG 1 cut(s) 90
AfaI GTAC 3 cut(s) 81, 672, 980
AfiI CCNNNNNNNGG 1 cut(s) 947
AflII CTTAAG 1 cut(s) 551
AgsI TTSAA 9 cut(s) 368, 574, 657, 746, 847, 947, 991, 1016, 1046
AjnI CCWGG 1 cut(s) 629
AluBI AGCT 3 cut(s) 224, 463, 776
AluI AGCT 3 cut(s) 224, 463, 776
Alw21I GWGCWC 2 cut(s) 778, 861
Alw26I GTCTC 2 cut(s) 176, 920
Alw44I GTGCAC 1 cut(s) 857
AlwNI CAGNNNCTG 1 cut(s) 545
AoxI GGCC 5 cut(s) 350, 473, 659, 949, 968
ApaLI GTGCAC 1 cut(s) 857
ApeKI GCWGC 2 cut(s) 400, 1049
ApoI RAATTY 3 cut(s) 203, 275, 574
AseI ATTAAT 1 cut(s) 804
Asp700I GAANNNNTTC 1 cut(s) 741
AspLEI GCGC 2 cut(s) 459, 565
AsuC2I CCSGG 1 cut(s) 917
AsuHPI GGTGA 2 cut(s) 700, 824
AxyI CCTNAGG 1 cut(s) 789
BaeGI GKGCMC 1 cut(s) 861
BanI GGYRCC 1 cut(s) 85
BanII GRGCYC 2 cut(s) 778, 854
BarI GAAGNNNNNNTAC 2 cut(s) 63, 95
BbsI GAAGAC 2 cut(s) 734, 1042
Bbv12I GWGCWC 2 cut(s) 778, 861
BbvI GCAGC 1 cut(s) 412
BccI CCATC 4 cut(s) 104, 139, 689, 752
BciT130I CCWGG 1 cut(s) 631
BciVI GTATCC 1 cut(s) 493
BclI TGATCA 1 cut(s) 814
BcnI CCSGG 1 cut(s) 917
BcoDI GTCTC 2 cut(s) 176, 920
BfaI CTAG 2 cut(s) 506, 674
BfrI CTTAAG 1 cut(s) 551
BfuI GTATCC 1 cut(s) 493
BisI GCNGC 2 cut(s) 401, 1050
BlsI GCNGC 2 cut(s) 402, 1051
BmcAI AGTACT 1 cut(s) 81
Bme1390I CCNGG 2 cut(s) 631, 917
BmiI GGNNCC 1 cut(s) 87
BmrFI CCNGG 2 cut(s) 631, 917
BmrI ACTGGG 1 cut(s) 848
BmsI GCATC 2 cut(s) 616, 1014
BmuI ACTGGG 1 cut(s) 848
BpiI GAAGAC 2 cut(s) 734, 1042
BpuEI CTTGAG 1 cut(s) 449
BpuMI CCSGG 1 cut(s) 917
BsaI GGTCTC 1 cut(s) 176
BsaJI CCNNGG 3 cut(s) 24, 259, 971
BsaWI WCCGGW 1 cut(s) 154
Bsc4I CCNNNNNNNGG 1 cut(s) 947
Bse1I ACTGG 4 cut(s) 87, 330, 519, 854
Bse21I CCTNAGG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 631
BseDI CCNNGG 3 cut(s) 24, 259, 971
BseGI GGATG 2 cut(s) 154, 631
BseLI CCNNNNNNNGG 1 cut(s) 947
BseMII CTCAG 2 cut(s) 229, 780
BseNI ACTGG 4 cut(s) 87, 330, 519, 854
BseRI GAGGAG 1 cut(s) 164
BseSI GKGCMC 1 cut(s) 861
BseXI GCAGC 1 cut(s) 412
Bsh1236I CGCG 2 cut(s) 565, 669
BshFI GGCC 5 cut(s) 352, 475, 661, 951, 970
BshNI GGYRCC 1 cut(s) 85
BsiHKAI GWGCWC 2 cut(s) 778, 861
BsiSI CCGG 2 cut(s) 155, 917
BslFI GGGAC 1 cut(s) 899
BslI CCNNNNNNNGG 1 cut(s) 947
BsmAI GTCTC 2 cut(s) 176, 920
BsmFI GGGAC 1 cut(s) 899
BsnI GGCC 5 cut(s) 352, 475, 661, 951, 970
Bso31I GGTCTC 1 cut(s) 176
Bsp1286I GDGCHC 3 cut(s) 778, 854, 861
Bsp1407I TGTACA 1 cut(s) 978
Bsp143I GATC 4 cut(s) 163, 218, 664, 814
Bsp19I CCATGG 1 cut(s) 259
BspACI CCGC 1 cut(s) 565
BspANI GGCC 5 cut(s) 352, 475, 661, 951, 970
BspCNI CTCAG 2 cut(s) 228, 781
BspFNI CGCG 2 cut(s) 565, 669
BspLI GGNNCC 1 cut(s) 87
BspT107I GGYRCC 1 cut(s) 85
BspTI CTTAAG 1 cut(s) 551
BspTNI GGTCTC 1 cut(s) 176
BsrGI TGTACA 1 cut(s) 978
BsrI ACTGG 4 cut(s) 87, 330, 519, 854
BssECI CCNNGG 3 cut(s) 24, 259, 971
BssMI GATC 4 cut(s) 163, 218, 664, 814
BssT1I CCWWGG 3 cut(s) 24, 259, 971
Bst2UI CCWGG 1 cut(s) 631
Bst4CI ACNGT 4 cut(s) 52, 439, 547, 843
Bst6I CTCTTC 1 cut(s) 324
BstAFI CTTAAG 1 cut(s) 551
BstAUI TGTACA 1 cut(s) 978
BstC8I GCNNGC 2 cut(s) 354, 461
BstDEI CTNAG 2 cut(s) 215, 789
BstDSI CCRYGG 1 cut(s) 259
BstF5I GGATG 2 cut(s) 154, 631
BstFNI CGCG 2 cut(s) 565, 669
BstHHI GCGC 2 cut(s) 459, 565
BstKTI GATC 4 cut(s) 166, 221, 667, 817
BstMAI GTCTC 2 cut(s) 176, 920
BstMBI GATC 4 cut(s) 163, 218, 664, 814
BstNI CCWGG 1 cut(s) 631
BstNSI RCATGY 1 cut(s) 692
BstSCI CCNGG 2 cut(s) 629, 915
BstSLI GKGCMC 1 cut(s) 861
BstUI CGCG 2 cut(s) 565, 669
BstV1I GCAGC 1 cut(s) 412
BstV2I GAAGAC 2 cut(s) 734, 1042
Bsu36I CCTNAGG 1 cut(s) 789
BsuI GTATCC 1 cut(s) 493
BsuRI GGCC 5 cut(s) 352, 475, 661, 951, 970
BtgI CCRYGG 1 cut(s) 259
BtsCI GGATG 2 cut(s) 154, 631
BtsIMutI CAGTG 3 cut(s) 66, 323, 861
Cac8I GCNNGC 2 cut(s) 354, 461
CaiI CAGNNNCTG 1 cut(s) 545
CfoI GCGC 2 cut(s) 459, 565
Csp6I GTAC 3 cut(s) 80, 671, 979
CviAII CATG 4 cut(s) 260, 385, 559, 689
CviQI GTAC 3 cut(s) 80, 671, 979
DdeI CTNAG 2 cut(s) 215, 789
DpnI GATC 4 cut(s) 165, 220, 666, 816
DpnII GATC 4 cut(s) 163, 218, 664, 814
Eam1104I CTCTTC 1 cut(s) 324
EarI CTCTTC 1 cut(s) 324
Ecl136II GAGCTC 1 cut(s) 776
Eco130I CCWWGG 3 cut(s) 24, 259, 971
Eco147I AGGCCT 2 cut(s) 661, 951
Eco24I GRGCYC 2 cut(s) 778, 854
Eco31I GGTCTC 1 cut(s) 176
Eco32I GATATC 1 cut(s) 934
Eco53kI GAGCTC 1 cut(s) 776
Eco57I CTGAAG 1 cut(s) 90
Eco81I CCTNAGG 1 cut(s) 789
EcoICRI GAGCTC 1 cut(s) 776
EcoRI GAATTC 1 cut(s) 275
EcoRII CCWGG 1 cut(s) 629
EcoRV GATATC 1 cut(s) 934
EcoT14I CCWWGG 3 cut(s) 24, 259, 971
EcoT38I GRGCYC 2 cut(s) 778, 854
ErhI CCWWGG 3 cut(s) 24, 259, 971
FaeI CATG 4 cut(s) 263, 388, 562, 692
FaqI GGGAC 1 cut(s) 899
FatI CATG 4 cut(s) 259, 384, 558, 688
FbaI TGATCA 1 cut(s) 814
Fnu4HI GCNGC 2 cut(s) 401, 1050
FokI GGATG 2 cut(s) 161, 638
FriOI GRGCYC 2 cut(s) 778, 854
Fsp4HI GCNGC 2 cut(s) 401, 1050
FspBI CTAG 2 cut(s) 506, 674
GlaI GCGC 2 cut(s) 458, 564
GluI GCNGC 2 cut(s) 401, 1050
HaeIII GGCC 5 cut(s) 352, 475, 661, 951, 970
HapII CCGG 2 cut(s) 155, 917
HhaI GCGC 2 cut(s) 459, 565
Hin1II CATG 4 cut(s) 263, 388, 562, 692
Hin6I GCGC 2 cut(s) 457, 563
HinP1I GCGC 2 cut(s) 457, 563
HinfI GANTC 5 cut(s) 8, 114, 268, 920, 962
HpaII CCGG 2 cut(s) 155, 917
HphI GGTGA 2 cut(s) 700, 824
Hpy166II GTNNAC 1 cut(s) 859
Hpy188I TCNGA 4 cut(s) 218, 397, 753, 925
Hpy188III TCNNGA 6 cut(s) 272, 301, 506, 596, 788, 847
Hpy8I GTNNAC 1 cut(s) 859
HpyAV CCTTC 6 cut(s) 65, 192, 362, 722, 731, 941
HpyCH4III ACNGT 4 cut(s) 52, 439, 547, 843
HpyCH4IV ACGT 2 cut(s) 905, 999
HpyCH4V TGCA 6 cut(s) 55, 317, 692, 721, 859, 878
HpyF3I CTNAG 2 cut(s) 215, 789
HpySE526I ACGT 2 cut(s) 905, 999
Hsp92II CATG 4 cut(s) 263, 388, 562, 692
HspAI GCGC 2 cut(s) 457, 563
Ksp22I TGATCA 1 cut(s) 814
Kzo9I GATC 4 cut(s) 163, 218, 664, 814
LmnI GCTCC 1 cut(s) 781
Lsp1109I GCAGC 1 cut(s) 412
LweI GCATC 2 cut(s) 616, 1014
MaeI CTAG 2 cut(s) 506, 674
MaeII ACGT 2 cut(s) 905, 999
MaeIII GTNAC 2 cut(s) 525, 868
MalI GATC 4 cut(s) 165, 220, 666, 816
MboI GATC 4 cut(s) 163, 218, 664, 814
MboII GAAGA 7 cut(s) 3, 212, 341, 596, 734, 1044, 1047
MfeI CAATTG 2 cut(s) 780, 1041
MhlI GDGCHC 3 cut(s) 778, 854, 861
MluCI AATT 9 cut(s) 203, 275, 308, 574, 746, 780, 801, 1041, 1053
MlyI GAGTC 2 cut(s) 262, 971
MnlI CCTC 8 cut(s) 185, 196, 342, 574, 657, 784, 882, 962
MroXI GAANNNNTTC 1 cut(s) 741
MseI TTAA 3 cut(s) 552, 636, 804
MslI CAYNNNNRTG 1 cut(s) 693
MspCI CTTAAG 1 cut(s) 551
MspI CCGG 2 cut(s) 155, 917
MspR9I CCNGG 2 cut(s) 631, 917
MunI CAATTG 2 cut(s) 780, 1041
MvaI CCWGG 1 cut(s) 631
MvnI CGCG 2 cut(s) 565, 669
NciI CCSGG 1 cut(s) 917
NcoI CCATGG 1 cut(s) 259
NdeII GATC 4 cut(s) 163, 218, 664, 814
NlaIII CATG 4 cut(s) 263, 388, 562, 692
NlaIV GGNNCC 1 cut(s) 87
NmuCI GTSAC 1 cut(s) 868
NspI RCATGY 1 cut(s) 692
PceI AGGCCT 2 cut(s) 661, 951
PdmI GAANNNNTTC 1 cut(s) 741
PfeI GAWTC 3 cut(s) 8, 114, 920
PflFI GACNNNGTC 1 cut(s) 287
PfoI TCCNGGA 1 cut(s) 915
PkrI GCNGC 2 cut(s) 402, 1051
PleI GAGTC 2 cut(s) 262, 970
PpsI GAGTC 2 cut(s) 262, 970
PshBI ATTAAT 1 cut(s) 804
Psp124BI GAGCTC 1 cut(s) 778
Psp6I CCWGG 1 cut(s) 629
PspGI CCWGG 1 cut(s) 629
PspN4I GGNNCC 1 cut(s) 87
PstNI CAGNNNCTG 1 cut(s) 545
PsyI GACNNNGTC 1 cut(s) 287
RsaI GTAC 3 cut(s) 81, 672, 980
RsaNI GTAC 3 cut(s) 80, 671, 979
RseI CAYNNNNRTG 1 cut(s) 693
SacI GAGCTC 1 cut(s) 778
SaqAI TTAA 3 cut(s) 552, 636, 804
SatI GCNGC 2 cut(s) 401, 1050
Sau3AI GATC 4 cut(s) 163, 218, 664, 814
ScaI AGTACT 1 cut(s) 81
SchI GAGTC 2 cut(s) 262, 971
ScrFI CCNGG 2 cut(s) 631, 917
SduI GDGCHC 3 cut(s) 778, 854, 861
SfaNI GCATC 2 cut(s) 616, 1014
SmiMI CAYNNNNRTG 1 cut(s) 693
SmlI CTYRAG 2 cut(s) 464, 551
SmoI CTYRAG 2 cut(s) 464, 551
Sse9I AATT 9 cut(s) 203, 275, 308, 574, 746, 780, 801, 1041, 1053
SseBI AGGCCT 2 cut(s) 661, 951
SsiI CCGC 1 cut(s) 565
SspI AATATT 1 cut(s) 344
SspMI CTAG 2 cut(s) 506, 674
SstI GAGCTC 1 cut(s) 778
StuI AGGCCT 2 cut(s) 661, 951
StyD4I CCNGG 2 cut(s) 629, 915
StyI CCWWGG 3 cut(s) 24, 259, 971
TaaI ACNGT 4 cut(s) 52, 439, 547, 843
TaiI ACGT 2 cut(s) 908, 1002
TaqI TCGA 2 cut(s) 30, 936
TasI AATT 9 cut(s) 203, 275, 308, 574, 746, 780, 801, 1041, 1053
TatI WGTACW 2 cut(s) 79, 978
TfiI GAWTC 3 cut(s) 8, 114, 920
Tru1I TTAA 3 cut(s) 552, 636, 804
Tru9I TTAA 3 cut(s) 552, 636, 804
TscAI CASTG 3 cut(s) 73, 330, 861
TseFI GTSAC 1 cut(s) 868
TseI GCWGC 2 cut(s) 400, 1049
Tsp45I GTSAC 1 cut(s) 868
TspGWI ACGGA 1 cut(s) 281
TspRI CASTG 3 cut(s) 73, 330, 861
Tth111I GACNNNGTC 1 cut(s) 287
Vha464I CTTAAG 1 cut(s) 551
VneI GTGCAC 1 cut(s) 857
VspI ATTAAT 1 cut(s) 804
XapI RAATTY 3 cut(s) 203, 275, 574
XbaI TCTAGA 1 cut(s) 505
XceI RCATGY 1 cut(s) 692
XmnI GAANNNNTTC 1 cut(s) 741
XspI CTAG 2 cut(s) 506, 674
ZrmI AGTACT 1 cut(s) 81
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.