Rw2G005500

phosphatase 2c

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0016726
Physical Location & Seq
Reverse (-)
5171915 .. 5177217
5303 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G005500.1

Sequence Viewer

Length: 1200 bp
ATGGAATCCACAGACCCCGATCCCCAACTCGACCGAGTCGACTCACTCGCCGCCGACCTCGAACTCGCCTCCGCCAGCTCCACCAGCCTCTCCTCCATACTCACCGCCGACGACTCCAGAAGCCCCACCAGCAGCTCCGGCGAGTTCTCCAGGACCACCACCAGCAGCTCCGGCGAGATTCCGGCGTTTCTGCCCCCTTCCGGGGAGGAGATAAGACCCCCGGCGGCGGTGGTTAGGCCGAAGTGCGTTGGGAGGAACAACAAGGGAGTGACGTGGGGGTTCACTTCGGTGATTGGGAGGAGGAGAGAGATGGAGGACGCGGTGGCTGTTATTCCCGGGTTTATGGCTCGCACGTGCGATCACGTGGGAGGTTGTACGGCTCCCGGTTCGAGAACCTCCTCCGAGATCTCCCCCGTCCATTTCTTCGGCGTCTACGACGGCCATGGCGGCAGTCAGGTGGCTAAATATTGTGCTGAGCGAATGCATGAAGTCATAGCACAAGAGTGGGATCGGGAGACGGTTGATGGCTGTGAATGGCAAAGGAGGTGGGAGACTACTTTCTTAGTTGGGTTCGAGAAGGCTGACAGTGAGGTCCTCACAGAGGCAGTTGCACCTGAGATGGTTGGATCAACTGCAGTTGTAGTTGTCCTATCTGGTTGTCAGATCATCACATCGAACTGTGGTGACTCTAGGGCAGTTCTTTGCCGAGGGACAGAAACAATCCCATTAACTGTTGATCAGAAGCCTGATAGAGAAGACGAACTCAAGAGAATTGAAGGAGAGGGAGGGAAAGTCATAAACTGGAATGGCGCAAGGGTGCTTGGAGTTCTTGCCATGTCGAGAGCTATAGGTGATCGATATCTAAGGCCTTGGATAATTCCAGTTCCTGAGATTACATTCACAGCCAGGACTGATGAGGATGAGTGTTTGATTTTGGCAAGTGATGGACTGTGGGATGTAATGACTAATGAAGAGGTTGGGGAGGTGGCTAGACATATTTTAAGAAGGCGGCGAAGGTCCATGTCTTCTAGTACTGATGATATTTCTGCAGCACAAGCTGTTGCTGATCATCTGACCGAAATTGCATATGGAAGAAATAGTTCCGACAACATATCCATTATAGTTGTTGATCTCAAAACGAAAAGAAAACGTCAGCCAAAATCCAGTTCTATTCTCACACCTTTTGAAATTCAGAGGTGA

Protein Analysis

399

Amino Acids

43.52

Weight (kDa)

5.06

Isoelectric Point (pI)

63.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 96 - 371 2.6e-60 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016726)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g06040
malus_domestica MD15G1195800.v1.1
prunus_persica Prupe.7G220600_v2.0.a1
pyrus_communis pycom15g17510
rosa_chinensis RchiOBHm_Chr2g0091801
rosa_laevigata RLG00000016252
rosa_multiflora Rmu_sc0021402.1_g000002
rosa_roxburghii Rroxscaffold_2G00149730
rosa_rugosa Rorug02G0021000 Rorug02G0021100
rosa_samantha Rh2BG065200 Rh2CG066600 Rh2DG064800
rosa_wichuraiana Rw2G005500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 590
AccI GTMKAC 2 cut(s) 39, 432
AccII CGCG 1 cut(s) 320
AciI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 447, 1009
AclWI GGATC 3 cut(s) 14, 516, 634
AcoI YGGCCR 1 cut(s) 439
AcsI RAATTY 1 cut(s) 1188
AcvI CACGTG 2 cut(s) 354, 364
AcyI GRCGYC 1 cut(s) 429
AfaI GTAC 2 cut(s) 376, 1033
AfiI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
AgsI TTSAA 2 cut(s) 776, 1187
AjiI CACGTC 1 cut(s) 273
AjnI CCWGG 2 cut(s) 149, 905
AjuI GAANNNNNNNTTGG 2 cut(s) 1150, 1182
AleI CACNNNNGTG 2 cut(s) 287, 502
AluBI AGCT 5 cut(s) 78, 135, 168, 845, 1058
AluI AGCT 5 cut(s) 78, 135, 168, 845, 1058
Alw26I GTCTC 2 cut(s) 509, 545
AlwI GGATC 3 cut(s) 14, 516, 634
AlwNI CAGNNNCTG 1 cut(s) 887
Ama87I CYCGRG 1 cut(s) 335
AoxI GGCC 3 cut(s) 236, 439, 866
ApeKI GCWGC 3 cut(s) 132, 165, 1049
ApoI RAATTY 1 cut(s) 1188
Asp700I GAANNNNTTC 1 cut(s) 1099
AspLEI GCGC 1 cut(s) 812
AspS9I GGNCC 3 cut(s) 153, 592, 1017
AsuC2I CCSGG 5 cut(s) 202, 221, 336, 337, 384
AsuHPI GGTGA 4 cut(s) 94, 301, 695, 863
AvaI CYCGRG 1 cut(s) 335
AvaII GGWCC 3 cut(s) 153, 592, 1017
BbrPI CACGTG 2 cut(s) 354, 364
BbsI GAAGAC 2 cut(s) 762, 1017
BbvI GCAGC 3 cut(s) 144, 177, 1061
BccI CCATC 4 cut(s) 304, 518, 613, 938
BceAI ACGGC 2 cut(s) 393, 454
BciT130I CCWGG 2 cut(s) 151, 907
BclI TGATCA 2 cut(s) 736, 1066
BcnI CCSGG 5 cut(s) 202, 221, 336, 337, 384
BcoDI GTCTC 2 cut(s) 509, 545
BfaI CTAG 3 cut(s) 690, 990, 1029
BfmI CTRYAG 3 cut(s) 633, 846, 1047
BglI GCCNNNNNGGC 1 cut(s) 447
BglII AGATCT 1 cut(s) 405
BisI GCNGC 7 cut(s) 51, 133, 166, 225, 448, 1010, 1050
BlpI GCTNAGC 1 cut(s) 474
BlsI GCNGC 7 cut(s) 52, 134, 167, 226, 449, 1011, 1051
BmcAI AGTACT 1 cut(s) 1033
Bme1390I CCNGG 7 cut(s) 151, 202, 221, 336, 337, 384, 907
Bme18I GGWCC 3 cut(s) 153, 592, 1017
BmeT110I CYCGRG 1 cut(s) 335
BmgBI CACGTC 1 cut(s) 273
BmgT120I GGNCC 3 cut(s) 153, 592, 1017
BmiI GGNNCC 1 cut(s) 381
BmrFI CCNGG 7 cut(s) 151, 202, 221, 336, 337, 384, 907
BpiI GAAGAC 2 cut(s) 762, 1017
BpmI CTGGAG 2 cut(s) 100, 133
Bpu1102I GCTNAGC 1 cut(s) 474
BpuEI CTTGAG 1 cut(s) 749
BpuMI CCSGG 5 cut(s) 202, 221, 336, 337, 384
Bsa29I ATCGAT 1 cut(s) 856
BsaAI YACGTR 2 cut(s) 354, 364
BsaBI GATNNNNATC 1 cut(s) 858
BsaHI GRCGYC 1 cut(s) 429
BsaJI CCNNGG 6 cut(s) 201, 219, 335, 442, 706, 869
BsaXI ACNNNNNCTCC 2 cut(s) 777, 807
Bsc4I CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
Bse1I ACTGG 3 cut(s) 806, 881, 1164
Bse8I GATNNNNATC 1 cut(s) 858
BseBI CCWGG 2 cut(s) 151, 907
BseCI ATCGAT 1 cut(s) 856
BseDI CCNNGG 6 cut(s) 201, 219, 335, 442, 706, 869
BseGI GGATG 2 cut(s) 925, 961
BseJI GATNNNNATC 1 cut(s) 858
BseLI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
BseMII CTCAG 3 cut(s) 465, 606, 879
BseNI ACTGG 3 cut(s) 806, 881, 1164
BseRI GAGGAG 5 cut(s) 82, 221, 313, 316, 388
BseXI GCAGC 3 cut(s) 144, 177, 1061
Bsh1236I CGCG 1 cut(s) 320
Bsh1285I CGRYCG 1 cut(s) 34
BshFI GGCC 3 cut(s) 238, 441, 868
BshVI ATCGAT 1 cut(s) 856
BsiEI CGRYCG 1 cut(s) 34
BsiHKCI CYCGRG 1 cut(s) 335
BsiSI CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
BslFI GGGAC 1 cut(s) 724
BslI CCNNNNNNNGG 5 cut(s) 200, 201, 202, 226, 601
BsmAI GTCTC 2 cut(s) 509, 545
BsmBI CGTCTC 1 cut(s) 509
BsmFI GGGAC 1 cut(s) 724
BsmI GAATGC 1 cut(s) 486
BsnI GGCC 3 cut(s) 238, 441, 868
BsoBI CYCGRG 1 cut(s) 335
Bsp1720I GCTNAGC 1 cut(s) 474
Bsp19I CCATGG 1 cut(s) 442
BspACI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 447, 1009
BspANI GGCC 3 cut(s) 238, 441, 868
BspCNI CTCAG 3 cut(s) 466, 607, 880
BspDI ATCGAT 1 cut(s) 856
BspFNI CGCG 1 cut(s) 320
BspLI GGNNCC 1 cut(s) 381
BspMAI CTGCAG 2 cut(s) 637, 1051
BspPI GGATC 3 cut(s) 14, 516, 634
BsrI ACTGG 3 cut(s) 806, 881, 1164
BssECI CCNNGG 6 cut(s) 201, 219, 335, 442, 706, 869
BssNI GRCGYC 1 cut(s) 429
BssT1I CCWWGG 2 cut(s) 442, 869
Bst2UI CCWGG 2 cut(s) 151, 907
Bst4CI ACNGT 5 cut(s) 520, 587, 680, 733, 951
Bst6I CTCTTC 1 cut(s) 966
BstACI GRCGYC 1 cut(s) 429
BstBAI YACGTR 2 cut(s) 354, 364
BstC8I GCNNGC 2 cut(s) 76, 349
BstDEI CTNAG 5 cut(s) 474, 562, 615, 863, 888
BstDSI CCRYGG 1 cut(s) 442
BstENI CCTNNNNNAGG 1 cut(s) 599
BstF5I GGATG 2 cut(s) 925, 961
BstFNI CGCG 1 cut(s) 320
BstHHI GCGC 1 cut(s) 812
BstMAI GTCTC 2 cut(s) 509, 545
BstMCI CGRYCG 1 cut(s) 34
BstMWI GCNNNNNNNGC 6 cut(s) 84, 129, 138, 171, 447, 1055
BstNI CCWGG 2 cut(s) 151, 907
BstSCI CCNGG 7 cut(s) 149, 200, 219, 334, 335, 382, 905
BstSFI CTRYAG 3 cut(s) 633, 846, 1047
BstUI CGCG 1 cut(s) 320
BstV1I GCAGC 3 cut(s) 144, 177, 1061
BstV2I GAAGAC 2 cut(s) 762, 1017
BstX2I RGATCY 1 cut(s) 405
BstYI RGATCY 1 cut(s) 405
Bsu15I ATCGAT 1 cut(s) 856
BsuRI GGCC 3 cut(s) 238, 441, 868
BsuTUI ATCGAT 1 cut(s) 856
BtgI CCRYGG 1 cut(s) 442
BtrI CACGTC 1 cut(s) 273
BtsCI GGATG 2 cut(s) 925, 961
BtsIMutI CAGTG 1 cut(s) 592
Cac8I GCNNGC 2 cut(s) 76, 349
CaiI CAGNNNCTG 1 cut(s) 887
CfoI GCGC 1 cut(s) 812
Cfr13I GGNCC 3 cut(s) 153, 592, 1017
Cfr9I CCCGGG 1 cut(s) 335
ClaI ATCGAT 1 cut(s) 856
CseI GACGC 2 cut(s) 326, 418
Csp6I GTAC 2 cut(s) 375, 1032
CspCI CAANNNNNGTGG 2 cut(s) 527, 562
CviAII CATG 4 cut(s) 443, 485, 835, 1021
CviQI GTAC 2 cut(s) 375, 1032
DdeI CTNAG 5 cut(s) 474, 562, 615, 863, 888
DrdI GACNNNNNNGTC 1 cut(s) 590
DseDI GACNNNNNNGTC 1 cut(s) 590
EaeI YGGCCR 1 cut(s) 439
Eam1104I CTCTTC 1 cut(s) 966
EarI CTCTTC 1 cut(s) 966
EciI GGCGGA 1 cut(s) 61
Eco130I CCWWGG 2 cut(s) 442, 869
Eco147I AGGCCT 1 cut(s) 868
Eco32I GATATC 1 cut(s) 860
Eco47I GGWCC 3 cut(s) 153, 592, 1017
Eco72I CACGTG 2 cut(s) 354, 364
Eco88I CYCGRG 1 cut(s) 335
EcoNI CCTNNNNNAGG 1 cut(s) 599
EcoO109I RGGNCCY 1 cut(s) 592
EcoRII CCWGG 2 cut(s) 149, 905
EcoRV GATATC 1 cut(s) 860
EcoT14I CCWWGG 2 cut(s) 442, 869
EcoT22I ATGCAT 1 cut(s) 486
ErhI CCWWGG 2 cut(s) 442, 869
Esp3I CGTCTC 1 cut(s) 509
FaeI CATG 4 cut(s) 446, 488, 838, 1024
FaqI GGGAC 1 cut(s) 724
FatI CATG 4 cut(s) 442, 484, 834, 1020
FauNDI CATATG 1 cut(s) 1087
FbaI TGATCA 2 cut(s) 736, 1066
FblI GTMKAC 2 cut(s) 39, 432
Fnu4HI GCNGC 7 cut(s) 51, 133, 166, 225, 448, 1010, 1050
FokI GGATG 2 cut(s) 932, 968
Fsp4HI GCNGC 7 cut(s) 51, 133, 166, 225, 448, 1010, 1050
FspBI CTAG 3 cut(s) 690, 990, 1029
GlaI GCGC 1 cut(s) 811
GluI GCNGC 7 cut(s) 51, 133, 166, 225, 448, 1010, 1050
GsuI CTGGAG 2 cut(s) 100, 133
HaeIII GGCC 3 cut(s) 238, 441, 868
HapII CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
HgaI GACGC 2 cut(s) 326, 418
HhaI GCGC 1 cut(s) 812
Hin1I GRCGYC 1 cut(s) 429
Hin1II CATG 4 cut(s) 446, 488, 838, 1024
Hin6I GCGC 1 cut(s) 810
HinP1I GCGC 1 cut(s) 810
HincII GTYRAC 1 cut(s) 40
HindII GTYRAC 1 cut(s) 40
HinfI GANTC 6 cut(s) 5, 36, 41, 113, 178, 686
HpaII CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
HphI GGTGA 4 cut(s) 94, 301, 695, 863
Hpy166II GTNNAC 3 cut(s) 40, 282, 433
Hpy188I TCNGA 6 cut(s) 403, 663, 741, 1074, 1105, 1194
Hpy188III TCNNGA 7 cut(s) 117, 390, 512, 574, 766, 840, 887
Hpy8I GTNNAC 3 cut(s) 40, 282, 433
Hpy99I CGWCG 2 cut(s) 113, 440
HpyAV CCTTC 5 cut(s) 207, 571, 770, 999, 1008
HpyCH4III ACNGT 5 cut(s) 520, 587, 680, 733, 951
HpyCH4IV ACGT 4 cut(s) 272, 353, 363, 1150
HpyCH4V TGCA 5 cut(s) 484, 611, 635, 1049, 1085
HpyF10VI GCNNNNNNNGC 6 cut(s) 84, 129, 138, 171, 447, 1055
HpyF3I CTNAG 5 cut(s) 474, 562, 615, 863, 888
HpySE526I ACGT 4 cut(s) 272, 353, 363, 1150
Hsp92I GRCGYC 1 cut(s) 429
Hsp92II CATG 4 cut(s) 446, 488, 838, 1024
HspAI GCGC 1 cut(s) 810
Ksp22I TGATCA 2 cut(s) 736, 1066
LmnI GCTCC 4 cut(s) 83, 140, 173, 385
Lsp1109I GCAGC 3 cut(s) 144, 177, 1061
MaeI CTAG 3 cut(s) 690, 990, 1029
MaeII ACGT 4 cut(s) 272, 353, 363, 1150
MaeIII GTNAC 2 cut(s) 268, 683
MboII GAAGA 5 cut(s) 415, 767, 983, 1017, 1104
MflI RGATCY 1 cut(s) 405
MluCI AATT 4 cut(s) 771, 876, 1080, 1188
MlyI GAGTC 4 cut(s) 35, 45, 107, 680
MmeI TCCRAC 2 cut(s) 604, 1128
Mph1103I ATGCAT 1 cut(s) 486
MroXI GAANNNNTTC 1 cut(s) 1099
MseI TTAA 2 cut(s) 728, 1001
MslI CAYNNNNRTG 2 cut(s) 287, 502
MspI CCGG 7 cut(s) 138, 171, 182, 201, 221, 336, 384
MspR9I CCNGG 7 cut(s) 151, 202, 221, 336, 337, 384, 907
Mva1269I GAATGC 1 cut(s) 486
MvaI CCWGG 2 cut(s) 151, 907
MvnI CGCG 1 cut(s) 320
MwoI GCNNNNNNNGC 6 cut(s) 84, 129, 138, 171, 447, 1055
NciI CCSGG 5 cut(s) 202, 221, 336, 337, 384
NcoI CCATGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 1087
NlaIII CATG 4 cut(s) 446, 488, 838, 1024
NlaIV GGNNCC 1 cut(s) 381
NmeAIII GCCGAG 1 cut(s) 731
NmuCI GTSAC 2 cut(s) 268, 683
NsiI ATGCAT 1 cut(s) 486
OliI CACNNNNGTG 2 cut(s) 287, 502
PceI AGGCCT 1 cut(s) 868
PcsI WCGNNNNNNNCGW 2 cut(s) 36, 432
PctI GAATGC 1 cut(s) 486
PdmI GAANNNNTTC 1 cut(s) 1099
PfeI GAWTC 2 cut(s) 5, 178
PflFI GACNNNGTC 1 cut(s) 35
PfoI TCCNGGA 1 cut(s) 149
PkrI GCNGC 7 cut(s) 52, 134, 167, 226, 449, 1011, 1051
PleI GAGTC 4 cut(s) 35, 44, 107, 680
PmaCI CACGTG 2 cut(s) 354, 364
PmlI CACGTG 2 cut(s) 354, 364
PpsI GAGTC 4 cut(s) 35, 44, 107, 680
Ppu21I YACGTR 2 cut(s) 354, 364
PpuMI RGGWCCY 1 cut(s) 592
Psp5II RGGWCCY 1 cut(s) 592
Psp6I CCWGG 2 cut(s) 149, 905
PspCI CACGTG 2 cut(s) 354, 364
PspGI CCWGG 2 cut(s) 149, 905
PspN4I GGNNCC 1 cut(s) 381
PspPI GGNCC 3 cut(s) 153, 592, 1017
PspPPI RGGWCCY 1 cut(s) 592
PstI CTGCAG 2 cut(s) 637, 1051
PstNI CAGNNNCTG 1 cut(s) 887
PsuI RGATCY 1 cut(s) 405
PsyI GACNNNGTC 1 cut(s) 35
RsaI GTAC 2 cut(s) 376, 1033
RsaNI GTAC 2 cut(s) 375, 1032
RseI CAYNNNNRTG 2 cut(s) 287, 502
SalI GTCGAC 1 cut(s) 38
SaqAI TTAA 2 cut(s) 728, 1001
SatI GCNGC 7 cut(s) 51, 133, 166, 225, 448, 1010, 1050
Sau96I GGNCC 3 cut(s) 153, 592, 1017
ScaI AGTACT 1 cut(s) 1033
SchI GAGTC 4 cut(s) 35, 45, 107, 680
ScrFI CCNGG 7 cut(s) 151, 202, 221, 336, 337, 384, 907
SfcI CTRYAG 3 cut(s) 633, 846, 1047
SinI GGWCC 3 cut(s) 153, 592, 1017
SmaI CCCGGG 1 cut(s) 337
SmiMI CAYNNNNRTG 2 cut(s) 287, 502
SmlI CTYRAG 1 cut(s) 764
SmoI CTYRAG 1 cut(s) 764
Sse9I AATT 4 cut(s) 771, 876, 1080, 1188
SseBI AGGCCT 1 cut(s) 868
SsiI CCGC 8 cut(s) 51, 72, 105, 224, 227, 320, 447, 1009
SspI AATATT 1 cut(s) 467
SspMI CTAG 3 cut(s) 690, 990, 1029
StuI AGGCCT 1 cut(s) 868
StyD4I CCNGG 7 cut(s) 149, 200, 219, 334, 335, 382, 905
StyI CCWWGG 2 cut(s) 442, 869
TaaI ACNGT 5 cut(s) 520, 587, 680, 733, 951
TaiI ACGT 4 cut(s) 275, 356, 366, 1153
TaqI TCGA 8 cut(s) 30, 39, 60, 389, 573, 674, 839, 856
TaqII GACCGA 2 cut(s) 48, 1091
TasI AATT 4 cut(s) 771, 876, 1080, 1188
TatI WGTACW 1 cut(s) 1031
TauI GCSGC 4 cut(s) 53, 227, 450, 1012
TfiI GAWTC 2 cut(s) 5, 178
Tru1I TTAA 2 cut(s) 728, 1001
Tru9I TTAA 2 cut(s) 728, 1001
TscAI CASTG 1 cut(s) 592
TseFI GTSAC 2 cut(s) 268, 683
TseI GCWGC 3 cut(s) 132, 165, 1049
Tsp45I GTSAC 2 cut(s) 268, 683
TspDTI ATGAA 2 cut(s) 501, 984
TspMI CCCGGG 1 cut(s) 335
TspRI CASTG 1 cut(s) 592
Tth111I GACNNNGTC 1 cut(s) 35
VpaK11BI GGWCC 3 cut(s) 153, 592, 1017
XagI CCTNNNNNAGG 1 cut(s) 599
XapI RAATTY 1 cut(s) 1188
XmaI CCCGGG 1 cut(s) 335
XmiI GTMKAC 2 cut(s) 39, 432
XmnI GAANNNNTTC 1 cut(s) 1099
XspI CTAG 3 cut(s) 690, 990, 1029
ZrmI AGTACT 1 cut(s) 1033
Zsp2I ATGCAT 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.