FvH4_1g08030

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0012542
Physical Location & Seq
Reverse (-)
4247743 .. 4249679
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g08030.t2

Sequence Viewer

Length: 624 bp
ATGGGGGAAGAAGCAGAGGGTTCTGTACGTGTGTCAGACAGTAACGGAGGTGATCATACTCAGAGCCGTCAACTTGACATATACCCTCTCAGTTGCTACTACTTTGGATCCAAGGAAGCCATTCCTTTCAAGGATGAGACTTTAGATGATCGTGTTCAGAGATTGAACTCCAATCTTATAACCCAGGTCGAGTTGTTCAAACATCCTCATATACTACTCTTGCAAGTAAGGAATTGCATTTTTAAGCTTCCTGGAGGTCGTTTGAGACCTGGTGAATTAGAAATTGATGGCTTGAAACGAAAACTATCTAGGAAGCTTTGTGTCAATGCTGATGGTGATGGGATTGCTTGGGAGGTTGGTGAATGCCTTGATGTATGGTGGAGGCCGGACTTTGAAACATCGCCATGTCGGGACTTGCCATCTACAGTGAGAAGTCCAAAGGAGCGCACGAAACTCTTTCTTGTAAAGTTGCCGGAAAGTCGGAAGTTCATTGTACCCAAGAACCTAAAATTGCTGGCTATTCCACTGTGCCAGATCAACAAAAATCGCGAGACATATGGACCTATTATATCCGGTGTTCCAGAGATGCTATCGAAGTTCACCATCAACATTATAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
NUDIX_2

Protein Analysis

208

Amino Acids

23.54

Weight (kDa)

8.18

Isoelectric Point (pI)

42.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_2 PF13869 24 - 211 2.1e-65 Nucleotide hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 179
AccII CGCG 1 cut(s) 549
AclWI GGATC 2 cut(s) 102, 115
AfaI GTAC 2 cut(s) 27, 495
AflIII ACRYGT 1 cut(s) 28
AgsI TTSAA 5 cut(s) 130, 166, 199, 295, 395
AjnI CCWGG 3 cut(s) 183, 250, 268
AluBI AGCT 3 cut(s) 247, 316, 620
AluI AGCT 3 cut(s) 247, 316, 620
Alw26I GTCTC 3 cut(s) 131, 259, 545
AlwI GGATC 2 cut(s) 102, 115
AoxI GGCC 1 cut(s) 383
Asp700I GAANNNNTTC 1 cut(s) 120
AspLEI GCGC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 560
AsuHPI GGTGA 5 cut(s) 62, 284, 347, 371, 592
AvaII GGWCC 1 cut(s) 560
BamHI GGATCC 1 cut(s) 107
BccI CCATC 5 cut(s) 281, 326, 332, 427, 611
BceAI ACGGC 1 cut(s) 51
BciT130I CCWGG 3 cut(s) 185, 252, 270
BclI TGATCA 1 cut(s) 52
BcoDI GTCTC 3 cut(s) 131, 259, 545
BfaI CTAG 1 cut(s) 309
BfmI CTRYAG 1 cut(s) 423
Bme1390I CCNGG 3 cut(s) 185, 252, 270
Bme18I GGWCC 1 cut(s) 560
BmgT120I GGNCC 1 cut(s) 560
BmiI GGNNCC 1 cut(s) 109
BmrFI CCNGG 3 cut(s) 185, 252, 270
BmsI GCATC 1 cut(s) 576
BpmI CTGGAG 1 cut(s) 273
BsaAI YACGTR 1 cut(s) 29
BsaI GGTCTC 1 cut(s) 259
BsaJI CCNNGG 2 cut(s) 111, 183
BsaWI WCCGGW 1 cut(s) 572
BseBI CCWGG 3 cut(s) 185, 252, 270
BseDI CCNNGG 2 cut(s) 111, 183
BseGI GGATG 2 cut(s) 139, 202
BseMII CTCAG 2 cut(s) 74, 103
Bsh1236I CGCG 1 cut(s) 549
BshFI GGCC 1 cut(s) 385
BsiSI CCGG 3 cut(s) 386, 473, 573
BslFI GGGAC 1 cut(s) 425
BsmAI GTCTC 3 cut(s) 131, 259, 545
BsmFI GGGAC 1 cut(s) 425
BsmI GAATGC 1 cut(s) 368
BsnI GGCC 1 cut(s) 385
Bso31I GGTCTC 1 cut(s) 259
Bsp143I GATC 4 cut(s) 52, 107, 148, 534
Bsp68I TCGCGA 1 cut(s) 549
BspANI GGCC 1 cut(s) 385
BspCNI CTCAG 2 cut(s) 73, 102
BspFNI CGCG 1 cut(s) 549
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 102, 115
BspTNI GGTCTC 1 cut(s) 259
BssECI CCNNGG 2 cut(s) 111, 183
BssMI GATC 4 cut(s) 52, 107, 148, 534
BssT1I CCWWGG 1 cut(s) 111
Bst2UI CCWGG 3 cut(s) 185, 252, 270
Bst4CI ACNGT 3 cut(s) 41, 427, 528
BstBAI YACGTR 1 cut(s) 29
BstC8I GCNNGC 1 cut(s) 516
BstDEI CTNAG 2 cut(s) 60, 89
BstF5I GGATG 2 cut(s) 139, 202
BstFNI CGCG 1 cut(s) 549
BstHHI GCGC 1 cut(s) 447
BstKTI GATC 4 cut(s) 55, 110, 151, 537
BstMAI GTCTC 3 cut(s) 131, 259, 545
BstMBI GATC 4 cut(s) 52, 107, 148, 534
BstNI CCWGG 3 cut(s) 185, 252, 270
BstSCI CCNGG 3 cut(s) 183, 250, 268
BstSFI CTRYAG 1 cut(s) 423
BstUI CGCG 1 cut(s) 549
BstX2I RGATCY 1 cut(s) 107
BstYI RGATCY 1 cut(s) 107
BsuRI GGCC 1 cut(s) 385
BtgZI GCGATG 1 cut(s) 384
BtsCI GGATG 2 cut(s) 139, 202
BtsIMutI CAGTG 2 cut(s) 432, 524
BtuMI TCGCGA 1 cut(s) 549
Cac8I GCNNGC 1 cut(s) 516
CfoI GCGC 1 cut(s) 447
Cfr13I GGNCC 1 cut(s) 560
CsiI ACCWGGT 1 cut(s) 268
Csp6I GTAC 2 cut(s) 26, 494
CviAII CATG 1 cut(s) 405
CviJI RGCY 8 cut(s) 66, 119, 247, 291, 316, 385, 518, 620
CviKI_1 RGCY 8 cut(s) 66, 119, 247, 291, 316, 385, 518, 620
CviQI GTAC 2 cut(s) 26, 494
DdeI CTNAG 2 cut(s) 60, 89
DpnI GATC 4 cut(s) 54, 109, 150, 536
DpnII GATC 4 cut(s) 52, 107, 148, 534
Eco130I CCWWGG 1 cut(s) 111
Eco31I GGTCTC 1 cut(s) 259
Eco47I GGWCC 1 cut(s) 560
EcoRII CCWGG 3 cut(s) 183, 250, 268
EcoT14I CCWWGG 1 cut(s) 111
ErhI CCWWGG 1 cut(s) 111
FaeI CATG 1 cut(s) 408
FaqI GGGAC 1 cut(s) 425
FatI CATG 1 cut(s) 404
FauNDI CATATG 1 cut(s) 556
FbaI TGATCA 1 cut(s) 52
FokI GGATG 2 cut(s) 146, 189
FspBI CTAG 1 cut(s) 309
GlaI GCGC 1 cut(s) 446
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 1 cut(s) 385
HapII CCGG 3 cut(s) 386, 473, 573
HhaI GCGC 1 cut(s) 447
Hin1II CATG 1 cut(s) 408
Hin6I GCGC 1 cut(s) 445
HinP1I GCGC 1 cut(s) 445
HincII GTYRAC 1 cut(s) 71
HindII GTYRAC 1 cut(s) 71
HindIII AAGCTT 3 cut(s) 245, 314, 618
HpaII CCGG 3 cut(s) 386, 473, 573
HphI GGTGA 5 cut(s) 62, 284, 347, 371, 592
Hpy166II GTNNAC 2 cut(s) 71, 600
Hpy188I TCNGA 4 cut(s) 37, 63, 159, 483
Hpy188III TCNNGA 3 cut(s) 410, 548, 581
Hpy8I GTNNAC 2 cut(s) 71, 600
HpyCH4III ACNGT 3 cut(s) 41, 427, 528
HpyCH4IV ACGT 1 cut(s) 28
HpyCH4V TGCA 2 cut(s) 223, 237
HpyF3I CTNAG 2 cut(s) 60, 89
HpySE526I ACGT 1 cut(s) 28
Hsp92II CATG 1 cut(s) 408
HspAI GCGC 1 cut(s) 445
Ksp22I TGATCA 1 cut(s) 52
Kzo9I GATC 4 cut(s) 52, 107, 148, 534
LmnI GCTCC 1 cut(s) 442
LweI GCATC 1 cut(s) 576
MabI ACCWGGT 1 cut(s) 268
MaeI CTAG 1 cut(s) 309
MaeII ACGT 1 cut(s) 28
MaeIII GTNAC 1 cut(s) 41
MalI GATC 4 cut(s) 54, 109, 150, 536
MboI GATC 4 cut(s) 52, 107, 148, 534
MboII GAAGA 1 cut(s) 20
MflI RGATCY 1 cut(s) 107
MluCI AATT 4 cut(s) 232, 275, 282, 509
MmeI TCCRAC 1 cut(s) 461
MnlI CCTC 7 cut(s) 10, 41, 96, 216, 248, 346, 375
MroXI GAANNNNTTC 1 cut(s) 120
MseI TTAA 1 cut(s) 243
MslI CAYNNNNRTG 1 cut(s) 403
MspI CCGG 3 cut(s) 386, 473, 573
MspR9I CCNGG 3 cut(s) 185, 252, 270
Mva1269I GAATGC 1 cut(s) 368
MvaI CCWGG 3 cut(s) 185, 252, 270
MvnI CGCG 1 cut(s) 549
NdeI CATATG 1 cut(s) 556
NdeII GATC 4 cut(s) 52, 107, 148, 534
NlaIII CATG 1 cut(s) 408
NlaIV GGNNCC 1 cut(s) 109
NruI TCGCGA 1 cut(s) 549
PctI GAATGC 1 cut(s) 368
PdmI GAANNNNTTC 1 cut(s) 120
PfoI TCCNGGA 1 cut(s) 250
Ppu21I YACGTR 1 cut(s) 29
PsiI TTATAA 1 cut(s) 179
Psp6I CCWGG 3 cut(s) 183, 250, 268
PspGI CCWGG 3 cut(s) 183, 250, 268
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 560
PsuI RGATCY 1 cut(s) 107
RruI TCGCGA 1 cut(s) 549
RsaI GTAC 2 cut(s) 27, 495
RsaNI GTAC 2 cut(s) 26, 494
RseI CAYNNNNRTG 1 cut(s) 403
SaqAI TTAA 1 cut(s) 243
Sau3AI GATC 4 cut(s) 52, 107, 148, 534
Sau96I GGNCC 1 cut(s) 560
ScrFI CCNGG 3 cut(s) 185, 252, 270
SexAI ACCWGGT 1 cut(s) 268
SfaNI GCATC 1 cut(s) 576
SfcI CTRYAG 1 cut(s) 423
SinI GGWCC 1 cut(s) 560
SmiMI CAYNNNNRTG 1 cut(s) 403
Sse9I AATT 4 cut(s) 232, 275, 282, 509
SspMI CTAG 1 cut(s) 309
StyD4I CCNGG 3 cut(s) 183, 250, 268
StyI CCWWGG 1 cut(s) 111
TaaI ACNGT 3 cut(s) 41, 427, 528
TaiI ACGT 1 cut(s) 31
TaqI TCGA 2 cut(s) 189, 593
TasI AATT 4 cut(s) 232, 275, 282, 509
Tru1I TTAA 1 cut(s) 243
Tru9I TTAA 1 cut(s) 243
TscAI CASTG 2 cut(s) 432, 531
TspDTI ATGAA 1 cut(s) 478
TspGWI ACGGA 1 cut(s) 60
TspRI CASTG 2 cut(s) 432, 531
VpaK11BI GGWCC 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 120
XspI CTAG 1 cut(s) 309
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.