MD02G1085100.v1.1

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0012542
Physical Location & Seq
Reverse (-)
6678514 .. 6681325
2812 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1085100.v1.1.491

Sequence Viewer

Length: 666 bp
ATGGGGGAAGAAAGGGGTTCCTTACACGTGTCAGACAGCAACGGTCTTGATCATCGTGACAACGATGATCAGAGCCGTACGCTGGACATATACCCTCTCAGCAGCTACTACTTCGCTTCCAAGGAAGCCATTCCTGTCAAGGTCGAAGCTCCGGCCGATCGTCTTCACAGATTAAAGTCCAATTATGCTGCTTATGGATTGAGGACTTGTGTGGAAGCAGTTATTCTGGTTGAGTTGTTCAAACATCCTCATGTGCTGCTCTTGCAAATAAGAAACTGCATTTTTAAGCTGCCCGGGGGCCGCTTAAGACCCGGCGAATCAGAGATCGACGGATTGAAACGTAAGCTGTCGAGGAAGCTATGTATCAATGCTGATGGTGATGGGATTCCTTGGGAGGTTGGGGAATGTCTTGGGGCATGGTGGAGGCACGACTTCGAATCATTGCTCTGCCGGGACTTGCCAAGTAGAGTTGAAAGCCCAAAGGAGTGCACAAAACTCTACGTTGTGCAGTTACCAGATTGTCGGAGGTTCATTGCGCCAAAAAACCAAAAGCTGCTTGCTATTCCCTTGTGCCAGATCGCCAAAAACAACGAGACGTATGGACCGATAATATCAGGTGTTCCAGAGATGCTATCGAAGTTTACCATCAACATTGTAGAAGATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
NUDIX_2

Protein Analysis

222

Amino Acids

25.01

Weight (kDa)

6.99

Isoelectric Point (pI)

42.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_2 PF13869 27 - 214 2.7e-65 Nucleotide hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 301
AcoI YGGCCR 1 cut(s) 153
AcvI CACGTG 1 cut(s) 28
AfaI GTAC 1 cut(s) 79
AfiI CCNNNNNNNGG 1 cut(s) 82
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 2 cut(s) 25, 27
AgsI TTSAA 3 cut(s) 241, 337, 473
AluBI AGCT 6 cut(s) 105, 149, 289, 346, 358, 553
AluI AGCT 6 cut(s) 105, 149, 289, 346, 358, 553
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 1 cut(s) 587
Alw44I GTGCAC 1 cut(s) 487
Ama87I CYCGRG 1 cut(s) 293
AoxI GGCC 2 cut(s) 153, 298
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 5 cut(s) 102, 188, 256, 289, 553
Asp700I GAANNNNTTC 1 cut(s) 129
AspLEI GCGC 1 cut(s) 538
AspS9I GGNCC 2 cut(s) 298, 602
AsuC2I CCSGG 4 cut(s) 294, 295, 312, 452
AsuHPI GGTGA 1 cut(s) 389
AsuII TTCGAA 1 cut(s) 435
AvaI CYCGRG 1 cut(s) 293
AvaII GGWCC 1 cut(s) 602
BaeGI GKGCMC 1 cut(s) 491
BbrPI CACGTG 1 cut(s) 28
BbsI GAAGAC 1 cut(s) 155
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 5 cut(s) 114, 175, 243, 276, 540
BccI CCATC 3 cut(s) 368, 374, 653
BceAI ACGGC 1 cut(s) 60
BclI TGATCA 2 cut(s) 49, 67
BcnI CCSGG 4 cut(s) 294, 295, 312, 452
BcoDI GTCTC 1 cut(s) 587
BfrI CTTAAG 1 cut(s) 304
BisI GCNGC 6 cut(s) 103, 189, 257, 290, 301, 554
BlsI GCNGC 6 cut(s) 104, 190, 258, 291, 302, 555
Bme1390I CCNGG 4 cut(s) 294, 295, 312, 452
Bme18I GGWCC 1 cut(s) 602
BmeT110I CYCGRG 1 cut(s) 293
BmgT120I GGNCC 2 cut(s) 298, 602
BmiI GGNNCC 2 cut(s) 19, 299
BmrFI CCNGG 4 cut(s) 294, 295, 312, 452
BmsI GCATC 1 cut(s) 618
BpiI GAAGAC 1 cut(s) 155
Bpu14I TTCGAA 1 cut(s) 435
BpuMI CCSGG 4 cut(s) 294, 295, 312, 452
BsaAI YACGTR 1 cut(s) 28
BsaJI CCNNGG 4 cut(s) 120, 293, 294, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse3DI GCAATG 2 cut(s) 440, 531
BseDI CCNNGG 4 cut(s) 120, 293, 294, 389
BseGI GGATG 1 cut(s) 244
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 2 cut(s) 440, 531
BseMII CTCAG 1 cut(s) 112
BseSI GKGCMC 1 cut(s) 491
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 5 cut(s) 114, 175, 243, 276, 540
BsgI GTGCAG 1 cut(s) 527
Bsh1285I CGRYCG 2 cut(s) 156, 160
BshFI GGCC 2 cut(s) 155, 300
BsiEI CGRYCG 2 cut(s) 156, 160
BsiHKAI GWGCWC 1 cut(s) 491
BsiHKCI CYCGRG 1 cut(s) 293
BsiSI CCGG 4 cut(s) 152, 294, 312, 451
BsiWI CGTACG 1 cut(s) 77
BslFI GGGAC 1 cut(s) 467
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 587
BsmBI CGTCTC 1 cut(s) 587
BsmFI GGGAC 1 cut(s) 467
BsnI GGCC 2 cut(s) 155, 300
BsoBI CYCGRG 1 cut(s) 293
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 5 cut(s) 49, 67, 157, 324, 576
BspACI CCGC 1 cut(s) 301
BspANI GGCC 2 cut(s) 155, 300
BspCNI CTCAG 1 cut(s) 111
BspLI GGNNCC 2 cut(s) 19, 299
BspT104I TTCGAA 1 cut(s) 435
BspTI CTTAAG 1 cut(s) 304
BsrDI GCAATG 2 cut(s) 440, 531
BssECI CCNNGG 4 cut(s) 120, 293, 294, 389
BssMI GATC 5 cut(s) 49, 67, 157, 324, 576
BssT1I CCWWGG 2 cut(s) 120, 389
Bst4CI ACNGT 1 cut(s) 44
BstAFI CTTAAG 1 cut(s) 304
BstBAI YACGTR 1 cut(s) 28
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 1 cut(s) 98
BstF5I GGATG 1 cut(s) 244
BstHHI GCGC 1 cut(s) 538
BstKTI GATC 5 cut(s) 52, 70, 160, 327, 579
BstMAI GTCTC 1 cut(s) 587
BstMBI GATC 5 cut(s) 49, 67, 157, 324, 576
BstMCI CGRYCG 2 cut(s) 156, 160
BstMWI GCNNNNNNNGC 1 cut(s) 262
BstSCI CCNGG 4 cut(s) 292, 293, 310, 450
BstSLI GKGCMC 1 cut(s) 491
BstV1I GCAGC 5 cut(s) 114, 175, 243, 276, 540
BstV2I GAAGAC 1 cut(s) 155
BstZI CGGCCG 1 cut(s) 153
BsuRI GGCC 2 cut(s) 155, 300
BtsCI GGATG 1 cut(s) 244
Cac8I GCNNGC 1 cut(s) 558
CfoI GCGC 1 cut(s) 538
Cfr13I GGNCC 2 cut(s) 298, 602
Cfr9I CCCGGG 1 cut(s) 293
Csp6I GTAC 1 cut(s) 78
CviAII CATG 2 cut(s) 251, 417
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 1 cut(s) 98
DpnI GATC 5 cut(s) 51, 69, 159, 326, 578
DpnII GATC 5 cut(s) 49, 67, 157, 324, 576
EaeI YGGCCR 1 cut(s) 153
EagI CGGCCG 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 153
Eco130I CCWWGG 2 cut(s) 120, 389
Eco47I GGWCC 1 cut(s) 602
Eco52I CGGCCG 1 cut(s) 153
Eco72I CACGTG 1 cut(s) 28
Eco88I CYCGRG 1 cut(s) 293
EcoT14I CCWWGG 2 cut(s) 120, 389
ErhI CCWWGG 2 cut(s) 120, 389
Esp3I CGTCTC 1 cut(s) 587
FaeI CATG 2 cut(s) 254, 420
FaiI YATR 8 cut(s) 89, 91, 186, 195, 252, 361, 418, 600
FaqI GGGAC 1 cut(s) 467
FatI CATG 2 cut(s) 250, 416
FbaI TGATCA 2 cut(s) 49, 67
Fnu4HI GCNGC 6 cut(s) 103, 189, 257, 290, 301, 554
FokI GGATG 1 cut(s) 231
Fsp4HI GCNGC 6 cut(s) 103, 189, 257, 290, 301, 554
GlaI GCGC 1 cut(s) 537
GluI GCNGC 6 cut(s) 103, 189, 257, 290, 301, 554
HaeIII GGCC 2 cut(s) 155, 300
HapII CCGG 4 cut(s) 152, 294, 312, 451
HhaI GCGC 1 cut(s) 538
Hin1II CATG 2 cut(s) 254, 420
Hin6I GCGC 1 cut(s) 536
HinP1I GCGC 1 cut(s) 536
HinfI GANTC 3 cut(s) 317, 385, 437
HpaII CCGG 4 cut(s) 152, 294, 312, 451
HphI GGTGA 1 cut(s) 389
Hpy166II GTNNAC 2 cut(s) 489, 642
Hpy188I TCNGA 4 cut(s) 34, 72, 322, 525
Hpy188III TCNNGA 3 cut(s) 47, 56, 623
Hpy8I GTNNAC 2 cut(s) 489, 642
Hpy99I CGWCG 1 cut(s) 332
HpyCH4III ACNGT 1 cut(s) 44
HpyCH4IV ACGT 4 cut(s) 27, 340, 501, 596
HpyCH4V TGCA 4 cut(s) 265, 279, 489, 508
HpyF10VI GCNNNNNNNGC 1 cut(s) 262
HpyF3I CTNAG 1 cut(s) 98
HpySE526I ACGT 4 cut(s) 27, 340, 501, 596
Hsp92II CATG 2 cut(s) 254, 420
HspAI GCGC 1 cut(s) 536
Ksp22I TGATCA 2 cut(s) 49, 67
Kzo9I GATC 5 cut(s) 49, 67, 157, 324, 576
LmnI GCTCC 1 cut(s) 154
Lsp1109I GCAGC 5 cut(s) 114, 175, 243, 276, 540
LweI GCATC 1 cut(s) 618
MaeII ACGT 4 cut(s) 27, 340, 501, 596
MaeIII GTNAC 2 cut(s) 56, 510
MalI GATC 5 cut(s) 51, 69, 159, 326, 578
MboI GATC 5 cut(s) 49, 67, 157, 324, 576
MboII GAAGA 2 cut(s) 20, 155
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 1 cut(s) 181
MmeI TCCRAC 1 cut(s) 503
MnlI CCTC 7 cut(s) 105, 195, 258, 345, 388, 417, 519
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 4 cut(s) 173, 285, 305, 664
MslI CAYNNNNRTG 1 cut(s) 249
MspCI CTTAAG 1 cut(s) 304
MspI CCGG 4 cut(s) 152, 294, 312, 451
MspR9I CCNGG 4 cut(s) 294, 295, 312, 452
MwoI GCNNNNNNNGC 1 cut(s) 262
NciI CCSGG 4 cut(s) 294, 295, 312, 452
NdeII GATC 5 cut(s) 49, 67, 157, 324, 576
NlaIII CATG 2 cut(s) 254, 420
NlaIV GGNNCC 2 cut(s) 19, 299
NmuCI GTSAC 1 cut(s) 56
NspV TTCGAA 1 cut(s) 435
PcsI WCGNNNNNNNCGW 1 cut(s) 602
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 3 cut(s) 317, 385, 437
Pfl23II CGTACG 1 cut(s) 77
PkrI GCNGC 6 cut(s) 104, 190, 258, 291, 302, 555
Ple19I CGATCG 1 cut(s) 160
PmaCI CACGTG 1 cut(s) 28
PmlI CACGTG 1 cut(s) 28
Ppu21I YACGTR 1 cut(s) 28
PspCI CACGTG 1 cut(s) 28
PspLI CGTACG 1 cut(s) 77
PspN4I GGNNCC 2 cut(s) 19, 299
PspPI GGNCC 2 cut(s) 298, 602
PvuI CGATCG 1 cut(s) 160
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
RseI CAYNNNNRTG 1 cut(s) 249
SaqAI TTAA 4 cut(s) 173, 285, 305, 664
SatI GCNGC 6 cut(s) 103, 189, 257, 290, 301, 554
Sau3AI GATC 5 cut(s) 49, 67, 157, 324, 576
Sau96I GGNCC 2 cut(s) 298, 602
ScrFI CCNGG 4 cut(s) 294, 295, 312, 452
SduI GDGCHC 1 cut(s) 491
SfaNI GCATC 1 cut(s) 618
SfuI TTCGAA 1 cut(s) 435
SinI GGWCC 1 cut(s) 602
SmaI CCCGGG 1 cut(s) 295
SmiMI CAYNNNNRTG 1 cut(s) 249
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 1 cut(s) 181
SsiI CCGC 1 cut(s) 301
StyD4I CCNGG 4 cut(s) 292, 293, 310, 450
StyI CCWWGG 2 cut(s) 120, 389
TaaI ACNGT 1 cut(s) 44
TaiI ACGT 4 cut(s) 30, 343, 504, 599
TaqI TCGA 5 cut(s) 144, 327, 350, 435, 635
TaqII GACCGA 1 cut(s) 619
TasI AATT 1 cut(s) 181
TauI GCSGC 1 cut(s) 303
TfiI GAWTC 3 cut(s) 317, 385, 437
Tru1I TTAA 4 cut(s) 173, 285, 305, 664
Tru9I TTAA 4 cut(s) 173, 285, 305, 664
TseFI GTSAC 1 cut(s) 56
TseI GCWGC 5 cut(s) 102, 188, 256, 289, 553
Tsp45I GTSAC 1 cut(s) 56
TspDTI ATGAA 1 cut(s) 520
TspGWI ACGGA 1 cut(s) 345
TspMI CCCGGG 1 cut(s) 293
Vha464I CTTAAG 1 cut(s) 304
VneI GTGCAC 1 cut(s) 487
VpaK11BI GGWCC 1 cut(s) 602
XmaI CCCGGG 1 cut(s) 293
XmnI GAANNNNTTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.