Rh2DG087500

Cleavage and polyadenylation specificity factor subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0012542
Physical Location & Seq
Reverse (-)
6793145 .. 6797203
4059 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG087500.1

Sequence Viewer

Length: 639 bp
ATGGGGGAAGAAGGGGGTTCTGTACGCGTGTCAGACAGTAACGTTGTTGATCATACTCACAACGATGATCTGAGCCGTCAACTGGACATATACCCTCTCAGTTGCTACTACTTTGGATCCAAGGAAGCCATTCCTTTCAAGGATGAGACTTTAGCTGATCGTGTTCAGAGATTGAACTCCAACTATGCTGCTTATGGATTGAGGACTTGTGTAGAAGCAGTTATTTTGGTCGAGTTATTCAAACATCCTCATATACTACTCTTGCAAGTAAGAAATTGCATCTTTAAGCTTCCTGGAGGTCGCTTAAGACCTGGTGAATTAGAGATTGATGGCTTGAAAAGAAAACTGTCGAGGAAGCTTTGTGTCAATGCTGATGCTGATGGGATTGCTTGGGAGGTGGGGGAATGCCTTGATGTATGGTGGAGGCCTGAATTCGAAACATTGCCTTGTCGAGACTTGCCGTCTACAGATAAAAACCCAAAGGAGTGCACGAAACTCTTTCTTGTAAAGTTGCCAGAAAGTCGCAAGTTCATTGTACCCAAAAACCTAAAATTGCTGGCTATTCCGTTGTGCCAGATCAACAAAAATCGTGAGGTAATTCATGAACTTCATAATTGTGCGGTATTTGTTTATGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
NUDIX_2

Protein Analysis

212

Amino Acids

24.26

Weight (kDa)

6.6

Isoelectric Point (pI)

38.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NUDIX_2 PF13869 27 - 198 6.3e-58 Nucleotide hydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 464
AccII CGCG 1 cut(s) 27
AciI CCGC 1 cut(s) 620
AclI AACGTT 1 cut(s) 42
AclWI GGATC 2 cut(s) 111, 124
AcsI RAATTY 1 cut(s) 431
AfaI GTAC 2 cut(s) 24, 537
AfiI CCNNNNNNNGG 1 cut(s) 82
AflII CTTAAG 1 cut(s) 304
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 4 cut(s) 139, 175, 241, 337
AhdI GACNNNNNGTC 1 cut(s) 460
AjnI CCWGG 2 cut(s) 292, 310
AluBI AGCT 3 cut(s) 155, 289, 358
AluI AGCT 3 cut(s) 155, 289, 358
Alw21I GWGCWC 1 cut(s) 491
Alw26I GTCTC 2 cut(s) 140, 447
Alw44I GTGCAC 1 cut(s) 487
AlwI GGATC 2 cut(s) 111, 124
AoxI GGCC 1 cut(s) 425
ApaLI GTGCAC 1 cut(s) 487
ApeKI GCWGC 1 cut(s) 188
ApoI RAATTY 1 cut(s) 431
Asp700I GAANNNNTTC 1 cut(s) 129
AsuHPI GGTGA 1 cut(s) 326
AsuII TTCGAA 1 cut(s) 435
BaeGI GKGCMC 1 cut(s) 491
BamHI GGATCC 1 cut(s) 116
Bbv12I GWGCWC 1 cut(s) 491
BbvI GCAGC 1 cut(s) 175
BccI CCATC 2 cut(s) 323, 374
BceAI ACGGC 2 cut(s) 60, 445
BciT130I CCWGG 2 cut(s) 294, 312
BclI TGATCA 1 cut(s) 49
BcoDI GTCTC 2 cut(s) 140, 447
BfmI CTRYAG 1 cut(s) 465
BfrI CTTAAG 1 cut(s) 304
BisI GCNGC 1 cut(s) 189
BlsI GCNGC 1 cut(s) 190
Bme1390I CCNGG 2 cut(s) 294, 312
BmeRI GACNNNNNGTC 1 cut(s) 460
BmiI GGNNCC 1 cut(s) 118
BmrFI CCNGG 2 cut(s) 294, 312
BmsI GCATC 2 cut(s) 288, 364
BpmI CTGGAG 1 cut(s) 315
Bpu14I TTCGAA 1 cut(s) 435
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 440
BseBI CCWGG 2 cut(s) 294, 312
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 148, 244
BseLI CCNNNNNNNGG 1 cut(s) 82
BseMI GCAATG 1 cut(s) 440
BseMII CTCAG 2 cut(s) 62, 112
BseNI ACTGG 1 cut(s) 87
BseSI GKGCMC 1 cut(s) 491
BseXI GCAGC 1 cut(s) 175
Bsh1236I CGCG 1 cut(s) 27
BshFI GGCC 1 cut(s) 427
BsiHKAI GWGCWC 1 cut(s) 491
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 2 cut(s) 140, 447
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 1 cut(s) 427
Bsp119I TTCGAA 1 cut(s) 435
Bsp1286I GDGCHC 1 cut(s) 491
Bsp143I GATC 5 cut(s) 49, 67, 116, 157, 576
BspACI CCGC 1 cut(s) 620
BspANI GGCC 1 cut(s) 427
BspCNI CTCAG 2 cut(s) 63, 111
BspFNI CGCG 1 cut(s) 27
BspHI TCATGA 1 cut(s) 601
BspLI GGNNCC 1 cut(s) 118
BspPI GGATC 2 cut(s) 111, 124
BspT104I TTCGAA 1 cut(s) 435
BspTI CTTAAG 1 cut(s) 304
BsrDI GCAATG 1 cut(s) 440
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 5 cut(s) 49, 67, 116, 157, 576
BssT1I CCWWGG 1 cut(s) 120
Bst2UI CCWGG 2 cut(s) 294, 312
Bst4CI ACNGT 2 cut(s) 38, 348
BstAFI CTTAAG 1 cut(s) 304
BstBI TTCGAA 1 cut(s) 435
BstC8I GCNNGC 1 cut(s) 558
BstDEI CTNAG 2 cut(s) 71, 98
BstF5I GGATG 2 cut(s) 148, 244
BstFNI CGCG 1 cut(s) 27
BstKTI GATC 5 cut(s) 52, 70, 119, 160, 579
BstMAI GTCTC 2 cut(s) 140, 447
BstMBI GATC 5 cut(s) 49, 67, 116, 157, 576
BstNI CCWGG 2 cut(s) 294, 312
BstSCI CCNGG 2 cut(s) 292, 310
BstSFI CTRYAG 1 cut(s) 465
BstSLI GKGCMC 1 cut(s) 491
BstUI CGCG 1 cut(s) 27
BstV1I GCAGC 1 cut(s) 175
BstX2I RGATCY 1 cut(s) 116
BstYI RGATCY 1 cut(s) 116
BsuRI GGCC 1 cut(s) 427
BtsCI GGATG 2 cut(s) 148, 244
Cac8I GCNNGC 1 cut(s) 558
CciI TCATGA 1 cut(s) 601
CsiI ACCWGGT 1 cut(s) 310
Csp6I GTAC 2 cut(s) 23, 536
CviAII CATG 1 cut(s) 602
CviJI RGCY 8 cut(s) 75, 128, 155, 289, 333, 358, 427, 560
CviKI_1 RGCY 8 cut(s) 75, 128, 155, 289, 333, 358, 427, 560
CviQI GTAC 2 cut(s) 23, 536
DdeI CTNAG 2 cut(s) 71, 98
DpnI GATC 5 cut(s) 51, 69, 118, 159, 578
DpnII GATC 5 cut(s) 49, 67, 116, 157, 576
DriI GACNNNNNGTC 1 cut(s) 460
Eam1105I GACNNNNNGTC 1 cut(s) 460
Eco130I CCWWGG 1 cut(s) 120
Eco147I AGGCCT 1 cut(s) 427
EcoRI GAATTC 1 cut(s) 431
EcoRII CCWGG 2 cut(s) 292, 310
EcoT14I CCWWGG 1 cut(s) 120
ErhI CCWWGG 1 cut(s) 120
FaeI CATG 1 cut(s) 605
FatI CATG 1 cut(s) 601
FbaI TGATCA 1 cut(s) 49
FblI GTMKAC 1 cut(s) 464
Fnu4HI GCNGC 1 cut(s) 189
FokI GGATG 2 cut(s) 155, 231
Fsp4HI GCNGC 1 cut(s) 189
GluI GCNGC 1 cut(s) 189
GsuI CTGGAG 1 cut(s) 315
HaeIII GGCC 1 cut(s) 427
Hin1II CATG 1 cut(s) 605
HincII GTYRAC 1 cut(s) 80
HindII GTYRAC 1 cut(s) 80
HindIII AAGCTT 2 cut(s) 287, 356
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 3 cut(s) 80, 465, 489
Hpy188I TCNGA 3 cut(s) 34, 72, 168
Hpy188III TCNNGA 3 cut(s) 452, 590, 602
Hpy8I GTNNAC 3 cut(s) 80, 465, 489
HpyAV CCTTC 1 cut(s) 5
HpyCH4III ACNGT 2 cut(s) 38, 348
HpyCH4IV ACGT 1 cut(s) 42
HpyCH4V TGCA 3 cut(s) 265, 279, 489
HpyF3I CTNAG 2 cut(s) 71, 98
HpySE526I ACGT 1 cut(s) 42
Hsp92II CATG 1 cut(s) 605
Ksp22I TGATCA 1 cut(s) 49
Kzo9I GATC 5 cut(s) 49, 67, 116, 157, 576
LpnPI CCDG 9 cut(s) 68, 279, 297, 306, 324, 441, 528, 542, 587
Lsp1109I GCAGC 1 cut(s) 175
LweI GCATC 2 cut(s) 288, 364
MabI ACCWGGT 1 cut(s) 310
MaeII ACGT 1 cut(s) 42
MaeIII GTNAC 1 cut(s) 38
MalI GATC 5 cut(s) 51, 69, 118, 159, 578
MboI GATC 5 cut(s) 49, 67, 116, 157, 576
MboII GAAGA 1 cut(s) 20
MflI RGATCY 1 cut(s) 116
MhlI GDGCHC 1 cut(s) 491
MluCI AATT 6 cut(s) 274, 317, 431, 551, 597, 613
MluI ACGCGT 1 cut(s) 25
MmeI TCCRAC 1 cut(s) 204
MnlI CCTC 8 cut(s) 105, 195, 258, 290, 345, 388, 417, 586
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 2 cut(s) 285, 305
MslI CAYNNNNRTG 2 cut(s) 63, 615
MspCI CTTAAG 1 cut(s) 304
MspR9I CCNGG 2 cut(s) 294, 312
Mva1269I GAATGC 1 cut(s) 410
MvaI CCWGG 2 cut(s) 294, 312
MvnI CGCG 1 cut(s) 27
NdeII GATC 5 cut(s) 49, 67, 116, 157, 576
NlaIII CATG 1 cut(s) 605
NlaIV GGNNCC 1 cut(s) 118
NspV TTCGAA 1 cut(s) 435
PagI TCATGA 1 cut(s) 601
PceI AGGCCT 1 cut(s) 427
PctI GAATGC 1 cut(s) 410
PdmI GAANNNNTTC 1 cut(s) 129
PfoI TCCNGGA 1 cut(s) 292
PkrI GCNGC 1 cut(s) 190
Psp1406I AACGTT 1 cut(s) 42
Psp6I CCWGG 2 cut(s) 292, 310
PspGI CCWGG 2 cut(s) 292, 310
PspN4I GGNNCC 1 cut(s) 118
PsuI RGATCY 1 cut(s) 116
RsaI GTAC 2 cut(s) 24, 537
RsaNI GTAC 2 cut(s) 23, 536
RseI CAYNNNNRTG 2 cut(s) 63, 615
SaqAI TTAA 2 cut(s) 285, 305
SatI GCNGC 1 cut(s) 189
Sau3AI GATC 5 cut(s) 49, 67, 116, 157, 576
ScrFI CCNGG 2 cut(s) 294, 312
SduI GDGCHC 1 cut(s) 491
SetI ASST 9 cut(s) 45, 157, 291, 301, 313, 360, 399, 549, 597
SexAI ACCWGGT 1 cut(s) 310
SfaNI GCATC 2 cut(s) 288, 364
SfcI CTRYAG 1 cut(s) 465
SfuI TTCGAA 1 cut(s) 435
SmiMI CAYNNNNRTG 2 cut(s) 63, 615
SmlI CTYRAG 1 cut(s) 304
SmoI CTYRAG 1 cut(s) 304
Sse9I AATT 6 cut(s) 274, 317, 431, 551, 597, 613
SseBI AGGCCT 1 cut(s) 427
SsiI CCGC 1 cut(s) 620
StuI AGGCCT 1 cut(s) 427
StyD4I CCNGG 2 cut(s) 292, 310
StyI CCWWGG 1 cut(s) 120
TaaI ACNGT 2 cut(s) 38, 348
TaiI ACGT 1 cut(s) 45
TaqI TCGA 4 cut(s) 231, 350, 435, 451
TasI AATT 6 cut(s) 274, 317, 431, 551, 597, 613
Tru1I TTAA 2 cut(s) 285, 305
Tru9I TTAA 2 cut(s) 285, 305
TseI GCWGC 1 cut(s) 188
TspDTI ATGAA 4 cut(s) 520, 590, 599, 618
TspGWI ACGGA 1 cut(s) 555
Vha464I CTTAAG 1 cut(s) 304
VneI GTGCAC 1 cut(s) 487
XapI RAATTY 1 cut(s) 431
XmiI GTMKAC 1 cut(s) 464
XmnI GAANNNNTTC 1 cut(s) 129
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.