Basic Information

Type: gene

Biological Identity

Species

fragaria_vesca

Chromosome

Fvb1

Orthogroup ID

OG0006629

Physical Location & Seq

Genomic Coordinates Reverse (-)

4730786 .. 4736159

Gene Structure 5374 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

FvH4_1g08920.t2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2214 bp

ATGAAGAAATCGACGTTACCGTTGCATAGCGCAACCGGCGCCGGTAAGAACCAGAAGCTCGGGAAGGAAGCGCTGGTGAAGCTGCTGAGGTGGCATTTCGGTCATCCGGAGTTCAGAGGGAAGCAATTGGAGGCCATTGAAGCTGTTCTATCAGGAAGAGACTGTTTCTGCTTGATGCCGACCGGTGGCGGGAAGTCCATGTGTTATCAGATTCCGGCGCTGGCCAAGAAGACCGGCATTGTGCTTGTTGTGTGTCCTTTGATAGCTTTGATGGAAAACCAAGTGATGGCGTTGAAGGAGAAAGGAATTGCTGCTGAATATCTATCTTCCACGCAGACATCCCAAGTCAAAAATAAGATACATGAAGACCTTGATTCTGGAAGACCGTCTTTGAGGCTTCTATATGTGACGCCCGAGTTGATTGCAACACCTGGATTTATGACAAAGCTGACAAAGATTCACTCAAGAGGGTTGTTGAATCTTATTGCCATAGATGAGGCACATTGCATCTCTTCATGGGGCCATGATTTTAGGCCTAGCTATCGTAAGCTATCGTCTTTAAGGAGTCACCTCCCAGGTGTGCCAACATTGGCTTTGACAGCTACAGCCGTTCCTAAAGTTCAGAAGGATGTGATGGAGTCCTTATGCCTCCAAAACCCTTTAGTCCTAAAATCATCTTTCAATCGTTCTAATATATATTATGAAGTTCGATACAAAGATCTTTTACATGATGTGTATGCTGATTTGTCTGACGTACTAAAATCTAGTGGAGATGTATGCGCAATTGTATACTGCCTTGAACGAACGATGTGCGATGACTTGTCTGTTCATCTATCACAAAACGGGATTTCATGTGCTGCTTATCATGCAGGGTTGAAGGATAAATTACGCAGTTCTGTTTTAGATGATTGGATTTCTTCCAAAATACAAGTTGTTGTGGCAACGGTGGCTTTTGGGATGGGTATAGATAGAAAAGATGTCAGAATTGTTTGCCATTTTAATATTCCAAAGTCAATGGAAGGCTTCTACCAAGAGTCTGGTAGAGCTGGTCGTGATCAATTACCCTCCAGAAGCCTATTGTATTATGGAATTGAAGATAGCAAAAGAATGAAATTTATTCTAAACAATGCTGAGAACAAGATATCAAAGGCCTCAAACTCACAGGAAGAGTTGTCTAAAAAATCCATGACCGACTTTCAACAGATGGTTGAGTATTGTGAGGGCTCTGGATGCCGGAGGAAAAAGATCCTTGAGAATTTTGGTGAAAAGGTACCTGTAACACTATGTAGCAAAACATGTGATGCATGTAAACATCCAAACCTCGTAGCCAAATACTTGGAGGAGCTCGCAACTTCTTGTATTGTACGGCAGAAAGCCGGCTCATCTAGAATTTTTGTGGGCAGCTCCTCAAATAAGGGTAATGAAGATCAGTCCTCTGAATTCTGGAACCGTGATGATGAAGCAAGTTGTTCTGAGGAAGACATATCTGATTCTGATGATGATGTTGCAGACCTTGCAGCTGTCAAGAGCCTTACCAGATCAAAGTTGATCGATGAAAAGTTGGAAGCCTTGCAGCGTGCAGAAGAAAAATATTACCAGAATAAATCCTCTGAAAAGCAGCCAGATAAACTTAACAAGAATGCTGTATCTGATACACTGAGGGAAGCAAGCAAGCAGAGATTAGAAAATTCTGTAAAGCAGGCTCAGCAACGACTTGGAAACTTAAAGATTGAGGTGGAAAAAGCGGCATCGTTCCTCGAAGATGAATGTTACAAAAAATATGGGAAAACTGGGAAATCATTCTACTATTCTCAAGTCGCAAGTACAGTGAGGTGGTTATCAACCGTAAATTCTACCGACTTAACTGATCGTCTTGGCACCATAACCAGTTCTCCGTCAGAGAATAGTGTGACAGTGCCTTCTGCAACACAATCACCATTGCTGGAGACAGGATCTTTTGCAAAGCCATCACCTTTGGTGGACAGAGCAACAGAAATCACCGTGAATGACTTCCATGAGAGTGTCAGACCAGAAGCATCTGTAGCTCTGTCACCAACGCAAAGTGATCCCCCAAGTACGATGTTGCCAGCAATTCCGTCCTTCTCAGAGTTTATCAACAGCAAAAAGGTGATGGACAACAGATCAAGCGGATCAGATAAGCAATCACCTAATGGGGGTAAGAAGAATCCAGAGAAGAGAATGAGGTTACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0000733 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0036310 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097617 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

DEAD Helicase_C RecQ_Zn_bind

Protein Analysis

738

Amino Acids

81.67

Weight (kDa)

8.62

Isoelectric Point (pI)

49.02

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	36 - 112	5.4e-07	DEAD/DEAH box helicase
Helicase_C	PF00271	152 - 257	9.3e-18	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	270 - 342	4.1e-13	RecQ zinc-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006629)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35740 AT4G35740
fragaria_vesca	FvH4_1g08920 FvH4_1g08920
malus_domestica	MD02G1094600.v1.1
prunus_persica	Prupe.7G196400_v2.0.a1 Prupe.7G196400_v2.0.a1 Prupe.7G196400_v2.0.a1
pyrus_communis	pycom02g07480
rosa_chinensis	RchiOBHm_Chr2g0095381
rosa_laevigata	RLG00000016560
rosa_multiflora	Rmu_co8503843.1_g000001 Rmu_ssc0000183.1_g000039
rosa_roxburghii	Rroxscaffold_2G00146420
rosa_rugosa	Rorug02G0050200 Rorug02G0050200 Rorug02G0050300
rosa_samantha	Rh2AG098400 Rh2AG098500 Rh2BG099600 Rh2BG099700 Rh2CG101600 Rh2CG101700 Rh2DG099200 Rh2DG099300
rosa_wichuraiana	Rw2G007430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	781
Acc65I	GGTACC	1 cut(s)	1270
AccB1I	GGYRCC	3 cut(s)	38, 1270, 1877
AccB7I	CCANNNNNTGG	1 cut(s)	286
AccI	GTMKAC	1 cut(s)	789
AccIII	TCCGGA	1 cut(s)	106
AciI	CCGC	3 cut(s)	189, 1745, 2148
AclWI	GGATC	4 cut(s)	1240, 1960, 2060, 2158
AcoI	YGGCCR	1 cut(s)	222
AcsI	RAATTY	6 cut(s)	1112, 1255, 1389, 1439, 1687, 1849
AcyI	GRCGYC	2 cut(s)	39, 410
AfaI	GTAC	5 cut(s)	756, 1272, 1365, 1825, 2077
AfeI	AGCGCT	1 cut(s)	72
AfiI	CCNNNNNNNGG	4 cut(s)	185, 189, 286, 2174
AflIII	ACRYGT	1 cut(s)	1295
AgeI	ACCGGT	1 cut(s)	182
AgsI	TTSAA	8 cut(s)	140, 295, 478, 682, 800, 877, 1094, 1199
AjnI	CCWGG	2 cut(s)	430, 574
Alw21I	GWGCWC	1 cut(s)	1347
Alw26I	GTCTC	2 cut(s)	153, 1940
AlwI	GGATC	4 cut(s)	1240, 1960, 2060, 2158
Ama87I	CYCGRG	2 cut(s)	59, 413
Aor13HI	TCCGGA	1 cut(s)	106
Aor51HI	AGCGCT	1 cut(s)	72
AoxI	GGCC	5 cut(s)	132, 222, 520, 533, 1149
ApeKI	GCWGC	7 cut(s)	82, 311, 857, 1401, 1517, 1573, 1618
ApoI	RAATTY	6 cut(s)	1112, 1255, 1389, 1439, 1687, 1849
AsiGI	ACCGGT	1 cut(s)	182
Asp700I	GAANNNNTTC	3 cut(s)	144, 1799, 2009
Asp718I	GGTACC	1 cut(s)	1270
AspLEI	GCGC	5 cut(s)	32, 41, 73, 220, 782
AspS9I	GGNCC	1 cut(s)	520
AsuHPI	GGTGA	9 cut(s)	88, 560, 1274, 1926, 1962, 1990, 2043, 2140, 2157
AvaI	CYCGRG	2 cut(s)	59, 413
BalI	TGGCCA	1 cut(s)	224
BanI	GGYRCC	3 cut(s)	38, 1270, 1877
BanII	GRGCYC	2 cut(s)	1226, 1347
BbsI	GAAGAC	4 cut(s)	236, 372, 388, 1485
Bbv12I	GWGCWC	1 cut(s)	1347
BbvCI	CCTCAGC	1 cut(s)	86
BbvI	GCAGC	7 cut(s)	69, 298, 844, 1413, 1529, 1585, 1630
BccI	CCATC	7 cut(s)	265, 280, 628, 952, 1198, 1975, 2125
BceAI	ACGGC	2 cut(s)	593, 1382
BcgI	CGANNNNNNTGC	4 cut(s)	404, 438, 792, 826
BciT130I	CCWGG	2 cut(s)	432, 576
BclI	TGATCA	1 cut(s)	1054
BcoDI	GTCTC	2 cut(s)	153, 1940
BfaI	CTAG	3 cut(s)	537, 765, 1386
BfmI	CTRYAG	2 cut(s)	603, 2040
BfoI	RGCGCY	3 cut(s)	42, 74, 221
BglII	AGATCT	1 cut(s)	718
BisI	GCNGC	8 cut(s)	83, 312, 858, 1402, 1518, 1574, 1619, 1746
BlpI	GCTNAGC	1 cut(s)	1704
BlsI	GCNGC	8 cut(s)	84, 313, 859, 1403, 1519, 1575, 1620, 1747
Bme1390I	CCNGG	2 cut(s)	432, 576
BmeT110I	CYCGRG	2 cut(s)	59, 413
BmgT120I	GGNCC	1 cut(s)	520
BmiI	GGNNCC	5 cut(s)	40, 521, 1272, 1448, 1879
BmrFI	CCNGG	2 cut(s)	432, 576
BmrI	ACTGGG	1 cut(s)	1800
BmsI	GCATC	6 cut(s)	165, 516, 1220, 1291, 1757, 2045
BmuI	ACTGGG	1 cut(s)	1800
BpiI	GAAGAC	4 cut(s)	236, 372, 388, 1485
BpmI	CTGGAG	2 cut(s)	1051, 1964
Bpu10I	CCTNAGC	1 cut(s)	86
Bpu1102I	GCTNAGC	1 cut(s)	1704
BpuEI	CTTGAG	3 cut(s)	448, 1271, 1797
Bsa29I	ATCGAT	1 cut(s)	1551
BsaHI	GRCGYC	2 cut(s)	39, 410
BsaJI	CCNNGG	1 cut(s)	574
BsaWI	WCCGGW	2 cut(s)	106, 182
BsaXI	ACNNNNNCTCC	2 cut(s)	1876, 1906
Bsc4I	CCNNNNNNNGG	4 cut(s)	185, 189, 286, 2174
Bse118I	RCCGGY	5 cut(s)	35, 41, 182, 233, 1376
Bse1I	ACTGG	2 cut(s)	1795, 1887
Bse3DI	GCAATG	2 cut(s)	502, 1937
BseAI	TCCGGA	1 cut(s)	106
BseBI	CCWGG	2 cut(s)	432, 576
BseCI	ATCGAT	1 cut(s)	1551
BseDI	CCNNGG	1 cut(s)	574
BseGI	GGATG	6 cut(s)	103, 338, 634, 963, 1235, 1312
BseLI	CCNNNNNNNGG	4 cut(s)	185, 189, 286, 2174
BseMI	GCAATG	2 cut(s)	502, 1937
BseMII	CTCAG	6 cut(s)	77, 1122, 1464, 1649, 1718, 2118
BseNI	ACTGG	2 cut(s)	1795, 1887
BseRI	GAGGAG	2 cut(s)	1355, 1396
BseXI	GCAGC	7 cut(s)	69, 298, 844, 1413, 1529, 1585, 1630
BsgI	GTGCAG	1 cut(s)	1599
Bsh1285I	CGRYCG	1 cut(s)	183
BshFI	GGCC	5 cut(s)	134, 224, 522, 535, 1151
BshNI	GGYRCC	3 cut(s)	38, 1270, 1877
BshTI	ACCGGT	1 cut(s)	182
BshVI	ATCGAT	1 cut(s)	1551
BsiEI	CGRYCG	1 cut(s)	183
BsiHKAI	GWGCWC	1 cut(s)	1347
BsiHKCI	CYCGRG	2 cut(s)	59, 413
BsiSI	CCGG	8 cut(s)	36, 42, 107, 183, 215, 234, 1234, 1377
BslI	CCNNNNNNNGG	4 cut(s)	185, 189, 286, 2174
BsmAI	GTCTC	2 cut(s)	153, 1940
BsmI	GAATGC	1 cut(s)	1645
BsnI	GGCC	5 cut(s)	134, 224, 522, 535, 1151
BsoBI	CYCGRG	2 cut(s)	59, 413
Bsp1286I	GDGCHC	2 cut(s)	1226, 1347
Bsp13I	TCCGGA	1 cut(s)	106
Bsp1720I	GCTNAGC	1 cut(s)	1704
BspACI	CCGC	3 cut(s)	189, 1745, 2148
BspANI	GGCC	5 cut(s)	134, 224, 522, 535, 1151
BspCNI	CTCAG	6 cut(s)	78, 1123, 1465, 1650, 1717, 2117
BspDI	ATCGAT	1 cut(s)	1551
BspEI	TCCGGA	1 cut(s)	106
BspLI	GGNNCC	5 cut(s)	40, 521, 1272, 1448, 1879
BspPI	GGATC	4 cut(s)	1240, 1960, 2060, 2158
BspT107I	GGYRCC	3 cut(s)	38, 1270, 1877
BsrDI	GCAATG	2 cut(s)	502, 1937
BsrFI	RCCGGY	5 cut(s)	35, 41, 182, 233, 1376
BsrI	ACTGG	2 cut(s)	1795, 1887
BssAI	RCCGGY	5 cut(s)	35, 41, 182, 233, 1376
BssECI	CCNNGG	1 cut(s)	574
BssNAI	GTATAC	1 cut(s)	790
BssNI	GRCGYC	2 cut(s)	39, 410
Bst1107I	GTATAC	1 cut(s)	790
Bst2UI	CCWGG	2 cut(s)	432, 576
Bst4CI	ACNGT	9 cut(s)	21, 164, 387, 946, 1451, 1828, 1846, 1915, 2002
Bst6I	CTCTTC	4 cut(s)	151, 517, 1161, 2189
BstACI	GRCGYC	2 cut(s)	39, 410
BstAPI	GCANNNNNTGC	1 cut(s)	1514
BstC8I	GCNNGC	8 cut(s)	222, 1347, 1378, 1578, 1669, 1673, 1701, 2088
BstDEI	CTNAG	6 cut(s)	86, 1131, 1473, 1658, 1704, 2104
BstF5I	GGATG	6 cut(s)	103, 338, 634, 963, 1235, 1312
BstH2I	RGCGCY	3 cut(s)	42, 74, 221
BstHHI	GCGC	5 cut(s)	32, 41, 73, 220, 782
BstMAI	GTCTC	2 cut(s)	153, 1940
BstMCI	CGRYCG	1 cut(s)	183
BstNI	CCWGG	2 cut(s)	432, 576
BstNSI	RCATGY	2 cut(s)	1299, 1308
BstSCI	CCNGG	2 cut(s)	430, 574
BstSFI	CTRYAG	2 cut(s)	603, 2040
BstV1I	GCAGC	7 cut(s)	69, 298, 844, 1413, 1529, 1585, 1630
BstV2I	GAAGAC	4 cut(s)	236, 372, 388, 1485
BstX2I	RGATCY	3 cut(s)	718, 1245, 1952
BstXI	CCANNNNNNTGG	2 cut(s)	1037, 1336
BstYI	RGATCY	3 cut(s)	718, 1245, 1952
BstZ17I	GTATAC	1 cut(s)	790
Bsu15I	ATCGAT	1 cut(s)	1551
BsuRI	GGCC	5 cut(s)	134, 224, 522, 535, 1151
BsuTUI	ATCGAT	1 cut(s)	1551
BtgZI	GCGATG	1 cut(s)	828
BtsCI	GGATG	6 cut(s)	103, 338, 634, 963, 1235, 1312
BtsIMutI	CAGTG	3 cut(s)	1655, 1833, 1920
Cac8I	GCNNGC	8 cut(s)	222, 1347, 1378, 1578, 1669, 1673, 1701, 2088
CfoI	GCGC	5 cut(s)	32, 41, 73, 220, 782
Cfr10I	RCCGGY	5 cut(s)	35, 41, 182, 233, 1376
Cfr13I	GGNCC	1 cut(s)	520
ClaI	ATCGAT	1 cut(s)	1551
CseI	GACGC	1 cut(s)	418
Csp6I	GTAC	5 cut(s)	755, 1271, 1364, 1824, 2076
CspAI	ACCGGT	1 cut(s)	182
CviQI	GTAC	5 cut(s)	755, 1271, 1364, 1824, 2076
DdeI	CTNAG	6 cut(s)	86, 1131, 1473, 1658, 1704, 2104
DinI	GGCGCC	1 cut(s)	40
EaeI	YGGCCR	1 cut(s)	222
Eam1104I	CTCTTC	4 cut(s)	151, 517, 1161, 2189
EarI	CTCTTC	4 cut(s)	151, 517, 1161, 2189
Ecl136II	GAGCTC	1 cut(s)	1345
Eco147I	AGGCCT	2 cut(s)	535, 1151
Eco24I	GRGCYC	2 cut(s)	1226, 1347
Eco32I	GATATC	1 cut(s)	1143
Eco47III	AGCGCT	1 cut(s)	72
Eco53kI	GAGCTC	1 cut(s)	1345
Eco88I	CYCGRG	2 cut(s)	59, 413
EcoICRI	GAGCTC	1 cut(s)	1345
EcoRI	GAATTC	1 cut(s)	1439
EcoRII	CCWGG	2 cut(s)	430, 574
EcoRV	GATATC	1 cut(s)	1143
EcoT22I	ATGCAT	1 cut(s)	1306
EcoT38I	GRGCYC	2 cut(s)	1226, 1347
EgeI	GGCGCC	1 cut(s)	40
EheI	GGCGCC	1 cut(s)	40
FalI	AAGNNNNNCTT	2 cut(s)	373, 405
FauI	CCCGC	1 cut(s)	182
FbaI	TGATCA	1 cut(s)	1054
FblI	GTMKAC	1 cut(s)	789
Fnu4HI	GCNGC	8 cut(s)	83, 312, 858, 1402, 1518, 1574, 1619, 1746
FokI	GGATG	6 cut(s)	90, 325, 641, 970, 1242, 1299
FriOI	GRGCYC	2 cut(s)	1226, 1347
Fsp4HI	GCNGC	8 cut(s)	83, 312, 858, 1402, 1518, 1574, 1619, 1746
FspBI	CTAG	3 cut(s)	537, 765, 1386
FspI	TGCGCA	1 cut(s)	781
GlaI	GCGC	5 cut(s)	31, 40, 72, 219, 781
GluI	GCNGC	8 cut(s)	83, 312, 858, 1402, 1518, 1574, 1619, 1746
GsuI	CTGGAG	2 cut(s)	1051, 1964
HaeII	RGCGCY	3 cut(s)	42, 74, 221
HaeIII	GGCC	5 cut(s)	134, 224, 522, 535, 1151
HapII	CCGG	8 cut(s)	36, 42, 107, 183, 215, 234, 1234, 1377
HgaI	GACGC	1 cut(s)	418
HhaI	GCGC	5 cut(s)	32, 41, 73, 220, 782
Hin1I	GRCGYC	2 cut(s)	39, 410
Hin6I	GCGC	5 cut(s)	30, 39, 71, 218, 780
HinP1I	GCGC	5 cut(s)	30, 39, 71, 218, 780
HinfI	GANTC	9 cut(s)	211, 374, 457, 478, 565, 638, 1034, 1490, 2185
HpaII	CCGG	8 cut(s)	36, 42, 107, 183, 215, 234, 1234, 1377
HphI	GGTGA	9 cut(s)	88, 560, 1274, 1926, 1962, 1990, 2043, 2140, 2157
Hpy166II	GTNNAC	3 cut(s)	790, 1310, 1981
Hpy8I	GTNNAC	3 cut(s)	790, 1310, 1981
Hpy99I	CGWCG	1 cut(s)	16
HpyAV	CCTTC	7 cut(s)	58, 289, 619, 871, 1013, 1929, 2110
HpyCH4III	ACNGT	9 cut(s)	21, 164, 387, 946, 1451, 1828, 1846, 1915, 2002
HpyCH4IV	ACGT	2 cut(s)	14, 753
HpyF3I	CTNAG	6 cut(s)	86, 1131, 1473, 1658, 1704, 2104
HpySE526I	ACGT	2 cut(s)	14, 753
Hsp92I	GRCGYC	2 cut(s)	39, 410
HspAI	GCGC	5 cut(s)	30, 39, 71, 218, 780
KasI	GGCGCC	1 cut(s)	38
Kpn2I	TCCGGA	1 cut(s)	106
KpnI	GGTACC	1 cut(s)	1274
KroI	GCCGGC	1 cut(s)	1376
KroNI	GCCGGC	1 cut(s)	1378
Ksp22I	TGATCA	1 cut(s)	1054
LmnI	GCTCC	2 cut(s)	1342, 1409
Lsp1109I	GCAGC	7 cut(s)	69, 298, 844, 1413, 1529, 1585, 1630
LweI	GCATC	6 cut(s)	165, 516, 1220, 1291, 1757, 2045
MaeI	CTAG	3 cut(s)	537, 765, 1386
MaeII	ACGT	2 cut(s)	14, 753
MaeIII	GTNAC	8 cut(s)	15, 406, 566, 1276, 1769, 1909, 2049, 2205
MfeI	CAATTG	2 cut(s)	125, 783
MflI	RGATCY	3 cut(s)	718, 1245, 1952
MhlI	GDGCHC	2 cut(s)	1226, 1347
MlsI	TGGCCA	1 cut(s)	224
MluNI	TGGCCA	1 cut(s)	224
Mly113I	GGCGCC	1 cut(s)	39
MlyI	GAGTC	3 cut(s)	574, 647, 1043
MmeI	TCCRAC	1 cut(s)	1542
Mox20I	TGGCCA	1 cut(s)	224
Mph1103I	ATGCAT	1 cut(s)	1306
MroI	TCCGGA	1 cut(s)	106
MroNI	GCCGGC	1 cut(s)	1376
MroXI	GAANNNNTTC	3 cut(s)	144, 1799, 2009
MscI	TGGCCA	1 cut(s)	224
MseI	TTAA	5 cut(s)	560, 999, 1632, 1724, 1862
MslI	CAYNNNNRTG	1 cut(s)	2019
Msp20I	TGGCCA	1 cut(s)	224
MspA1I	CMGCKG	1 cut(s)	1520
MspI	CCGG	8 cut(s)	36, 42, 107, 183, 215, 234, 1234, 1377
MspR9I	CCNGG	2 cut(s)	432, 576
MunI	CAATTG	2 cut(s)	125, 783
Mva1269I	GAATGC	1 cut(s)	1645
MvaI	CCWGG	2 cut(s)	432, 576
NaeI	GCCGGC	1 cut(s)	1378
NarI	GGCGCC	1 cut(s)	39
NgoMIV	GCCGGC	1 cut(s)	1376
NlaIV	GGNNCC	5 cut(s)	40, 521, 1272, 1448, 1879
NmuCI	GTSAC	4 cut(s)	406, 566, 1909, 2049
NsbI	TGCGCA	1 cut(s)	781
NsiI	ATGCAT	1 cut(s)	1306
NspI	RCATGY	2 cut(s)	1299, 1308
PceI	AGGCCT	2 cut(s)	535, 1151
PciI	ACATGT	1 cut(s)	1295
PcsI	WCGNNNNNNNCGW	1 cut(s)	17
PctI	GAATGC	1 cut(s)	1645
PdiI	GCCGGC	1 cut(s)	1378
PdmI	GAANNNNTTC	3 cut(s)	144, 1799, 2009
PfeI	GAWTC	6 cut(s)	211, 374, 457, 478, 1490, 2185
PflMI	CCANNNNNTGG	1 cut(s)	286
PinAI	ACCGGT	1 cut(s)	182
PkrI	GCNGC	8 cut(s)	84, 313, 859, 1403, 1519, 1575, 1620, 1747
PleI	GAGTC	3 cut(s)	573, 646, 1042
PluTI	GGCGCC	1 cut(s)	42
PpsI	GAGTC	3 cut(s)	573, 646, 1042
PscI	ACATGT	1 cut(s)	1295
Psp124BI	GAGCTC	1 cut(s)	1347
Psp6I	CCWGG	2 cut(s)	430, 574
PspGI	CCWGG	2 cut(s)	430, 574
PspN4I	GGNNCC	5 cut(s)	40, 521, 1272, 1448, 1879
PspPI	GGNCC	1 cut(s)	520
PsuI	RGATCY	3 cut(s)	718, 1245, 1952
PvuII	CAGCTG	1 cut(s)	1520
RsaI	GTAC	5 cut(s)	756, 1272, 1365, 1825, 2077
RsaNI	GTAC	5 cut(s)	755, 1271, 1364, 1824, 2076
RseI	CAYNNNNRTG	1 cut(s)	2019
SacI	GAGCTC	1 cut(s)	1347
SaqAI	TTAA	5 cut(s)	560, 999, 1632, 1724, 1862
SatI	GCNGC	8 cut(s)	83, 312, 858, 1402, 1518, 1574, 1619, 1746
Sau96I	GGNCC	1 cut(s)	520
SchI	GAGTC	3 cut(s)	574, 647, 1043
ScrFI	CCNGG	2 cut(s)	432, 576
SduI	GDGCHC	2 cut(s)	1226, 1347
SfaNI	GCATC	6 cut(s)	165, 516, 1220, 1291, 1757, 2045
SfcI	CTRYAG	2 cut(s)	603, 2040
SfoI	GGCGCC	1 cut(s)	40
SmiMI	CAYNNNNRTG	1 cut(s)	2019
SmlI	CTYRAG	3 cut(s)	463, 1250, 1812
SmoI	CTYRAG	3 cut(s)	463, 1250, 1812
SseBI	AGGCCT	2 cut(s)	535, 1151
SsiI	CCGC	3 cut(s)	189, 1745, 2148
SspDI	GGCGCC	1 cut(s)	38
SspI	AATATT	2 cut(s)	1003, 1592
SspMI	CTAG	3 cut(s)	537, 765, 1386
SstI	GAGCTC	1 cut(s)	1347
StuI	AGGCCT	2 cut(s)	535, 1151
StyD4I	CCNGG	2 cut(s)	430, 574
TaaI	ACNGT	9 cut(s)	21, 164, 387, 946, 1451, 1828, 1846, 1915, 2002
TaiI	ACGT	2 cut(s)	17, 756
TaqI	TCGA	4 cut(s)	11, 709, 1551, 1758
TaqII	GACCGA	2 cut(s)	89, 1205
TatI	WGTACW	1 cut(s)	1823
TauI	GCSGC	1 cut(s)	1748
TfiI	GAWTC	6 cut(s)	211, 374, 457, 478, 1490, 2185
Tru1I	TTAA	5 cut(s)	560, 999, 1632, 1724, 1862
Tru9I	TTAA	5 cut(s)	560, 999, 1632, 1724, 1862
TscAI	CASTG	3 cut(s)	1662, 1833, 1920
TseFI	GTSAC	4 cut(s)	406, 566, 1909, 2049
TseI	GCWGC	7 cut(s)	82, 311, 857, 1401, 1517, 1573, 1618
Tsp45I	GTSAC	4 cut(s)	406, 566, 1909, 2049
TspGWI	ACGGA	2 cut(s)	1884, 2085
TspRI	CASTG	3 cut(s)	1662, 1833, 1920
Van91I	CCANNNNNTGG	1 cut(s)	286
XapI	RAATTY	6 cut(s)	1112, 1255, 1389, 1439, 1687, 1849
XbaI	TCTAGA	1 cut(s)	1385
XceI	RCATGY	2 cut(s)	1299, 1308
XmiI	GTMKAC	1 cut(s)	789
XmnI	GAANNNNTTC	3 cut(s)	144, 1799, 2009
XspI	CTAG	3 cut(s)	537, 765, 1386
Zsp2I	ATGCAT	1 cut(s)	1306

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.