Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr4

Orthogroup ID

OG0006629

Physical Location & Seq

Genomic Coordinates Reverse (-)

7657751 .. 7663391

Gene Structure 5641 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000016560

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2334 bp

ATGTCCTCGGAAATTCCGGTTCACGAATCACGACCAAGCTCCGTCGTCTCTAAAAGTCTAAACCACACAAAACCAGTGACTTTTCTCAAACTCGAGGTTGAGGTCGACGCTTACTTGGCTCGCTTGAGAATGAAGAAATCACCGTTGCCATTACAGAACGCAAGCGGCGCGAGTAAGAATCAGAAGCTCGGAAAGGAAGCGCTGGTGAAGCTACTGAGATGGCATTTCGGTCATCCGGAATTCAGAGGGAAGCAATTGGAGGCCATTGAAGCTGTTCTATCAGGAAGAGATTGTTTCTGCTTGATGCCAACCGGAGGCGGGAAGTCGATGTGTTATCAGATACCGGCGCTGGCCAAGAAGACCGGAATCGTGCTTGTTGTGTGTCCTTTGATAGCTTTGATGGAAAACCAAGTGATGGCTTTGAAGGAGAAAGGAATTGCTGCTGAATATCTATCCTCCACACAGACTACACAAGTCAAAAGTAAGATACATGAAGACCTTGATTCTGGAAAACCGTCTTTGAGGCTGCTATATGTGACGCCGGAATTGATTGCAACACCTGGATTTATGACAAAGCTGACAAAGATTCACTCGAGAGGGTTGTTAAATCTTATTGCCATAGACGAGGCACATTGCATCTCTTCATGGGGCCATGATTTTAGGCCTAGCTATCGAAAGCTGTCGTCTTTGAGGAGCCACCTCCCAGGTGTACCAACATTAGCTTTGACAGCTACAGCCGTTCCTAAAGTTCAGAAGGATGTGATAGAGTCCTTACGCCTCCAAAACCCTTTAGTCCTAAAATCTTCTTTCAATCGTTCTAATATATATTATGAAGTTCGATACAAAGATCTTTTACACGATGTGTATGCTGATTTATCTGACGTACTAAAATCTAGTGGAGATATCTGTGCAATTGTCTACTGCCTTGAACGAACAATGTGCGATGACTTGTCTGTTCATCTATCACAAAATGGGATATCATGTGCTGCTTATCATGCAGGGTTGAATGATAAATTACGCAGTTCAGTTTTAGATGATTGGATTTCTTCTAAAATACAAGTTGTCGTGGCAACGGTCGCTTTTGGGATGGGTATAGATAGAAAAGATGTCAGAATTGTTTGCCATTTTAATATTCCGAAGTCAATGGAAGGCTTCTACCAAGAGTCTGGTAGAGCTGGTCGTGATCAATTACCCTCCAGAAGCCTATTGTATTATGGAATTGAAGATTGCAAGAGAATGAAATTTATTCTAAGCAATGCTGAGAACAAGAAATCAAAGGCCTCAAACTCACAAGAAGAGTTGTCAAAAAAATCCCTGACCGACTTTCAACAGATGGTTGAGTATTGTGAGGGCTCTGGATGCCGGAGGAAAAAGATCCTTGAGAATTTTGGTGAAAAGGTACCTGTAACACTGTGTAGCAAAACATGTGATGCCTGCAAACATCCAAACCTCGTAGCCAAATACTTGGAGGAGCTCGCAACTGCTTGTATCGTCCGGCAGAAAGCTGGTTCATCTCGAATTTTTGTGGGCAGCTCCTCAAATAAGGTTAATGAAGATCAGTCCTCTGAATTCTGGAACCGTGATGATGAAGCAAGTTGTTCTGAGGAAGACATATCTGATTCTGATGATGATGTTACGGACCTTGTAGCTGTCAAGAGCCTTACCAGATCAAAGTTGATCGATGAAAAATTGGAAGCCTTGCAGCGTGCAGAAGAAAAATATTACCAGAATAAATCTGATATGCAGACGGATAAACTTAACAAGATTGCTATATCTGATACGCTGAGAGAAGCAAGCAAGCAGAGATTAGAAAATGCTGTAAAGCAGGCTCAGCAACGACTTGGAAACTTAAAGATTGAGGTTGAAAACTCGGCATCTTTCCTTGAAAATGAATGTTACAAAAAATATGGGAAAACTGGGAAATCATTCTATTATTCTCAGGTCGCAAGTACAGTGAGGTGGTTATCAACCGTAAATTCTACCGACTTAACTGATCGTCTCGGCACCATAACCAGTTCCCCGTCAGAGAATACTCTGCTCAAAACAGCACCTTCTGCATCACAATTGCCATTGGTGCAGACAGGACCTTTTGCAACGCCATCACCTTTGGTGGACAGAGCAACAGAAATCACCAGAAATGACTTACATGAGAGTGTTAGACTGGAAGCTTCTGTAGCTCTGTCACCAACTCAAAGTGATTCCCCAAGTACAATTTTGCCAGCAATTCCATCCTTCTCAGAGTTTATCAACAGCAAAAAGGTGAACGACAGCAGATCAAGTGCATCCCCTAATGGGGTTAAGAAGAATCCAGAGAAGAAAATGAGGTTACAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000724 GO:0000725 GO:0000733 GO:0003674 GO:0003676 GO:0003677 GO:0003678 GO:0003824 GO:0004003 GO:0004386 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005694 GO:0005737 GO:0006139 GO:0006259 GO:0006281 GO:0006302 GO:0006310 GO:0006725 GO:0006807 GO:0006950 GO:0006974 GO:0006996 GO:0008026 GO:0008094 GO:0008150 GO:0008152 GO:0009378 GO:0009987 GO:0016043 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017111 GO:0032392 GO:0032508 GO:0033554 GO:0034641 GO:0036310 GO:0042623 GO:0043138 GO:0043140 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0044237 GO:0044238 GO:0044260 GO:0044424 GO:0044464 GO:0046483 GO:0050896 GO:0051276 GO:0051716 GO:0070035 GO:0071103 GO:0071704 GO:0071840 GO:0090304 GO:0097159 GO:0097617 GO:0140097 GO:1901360 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko03440 ko03460 map03440 map03460

Pfam Domains

Protein Families

DEAD Helicase_C RecQ_Zn_bind

Protein Analysis

778

Amino Acids

86.26

Weight (kDa)

8.77

Isoelectric Point (pI)

49.17

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DEAD	PF00270	85 - 250	3.5e-26	DEAD/DEAH box helicase
Helicase_C	PF00271	290 - 395	2.3e-17	Helicase conserved C-terminal domain
RecQ_Zn_bind	PF16124	408 - 480	2.5e-12	RecQ zinc-binding

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006629)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT4G35740 AT4G35740
fragaria_vesca	FvH4_1g08920 FvH4_1g08920
malus_domestica	MD02G1094600.v1.1
prunus_persica	Prupe.7G196400_v2.0.a1 Prupe.7G196400_v2.0.a1 Prupe.7G196400_v2.0.a1
pyrus_communis	pycom02g07480
rosa_chinensis	RchiOBHm_Chr2g0095381
rosa_laevigata	RLG00000016560
rosa_multiflora	Rmu_co8503843.1_g000001 Rmu_ssc0000183.1_g000039
rosa_roxburghii	Rroxscaffold_2G00146420
rosa_rugosa	Rorug02G0050200 Rorug02G0050200 Rorug02G0050300
rosa_samantha	Rh2AG098400 Rh2AG098500 Rh2BG099600 Rh2BG099700 Rh2CG101600 Rh2CG101700 Rh2DG099200 Rh2DG099300
rosa_wichuraiana	Rw2G007430

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	1399
AccB1I	GGYRCC	2 cut(s)	1399, 2003
AccB7I	CCANNNNNTGG	1 cut(s)	415
AccI	GTMKAC	2 cut(s)	105, 918
AccII	CGCG	1 cut(s)	170
AccIII	TCCGGA	1 cut(s)	235
AciI	CCGC	2 cut(s)	165, 318
AclWI	GGATC	1 cut(s)	1369
AcoI	YGGCCR	1 cut(s)	351
AcsI	RAATTY	7 cut(s)	12, 239, 1241, 1384, 1518, 1568, 1975
AcyI	GRCGYC	1 cut(s)	539
AdeI	CACNNNGTG	1 cut(s)	862
AfaI	GTAC	5 cut(s)	711, 885, 1401, 1951, 2209
AfeI	AGCGCT	1 cut(s)	201
AfiI	CCNNNNNNNGG	5 cut(s)	314, 318, 415, 2292, 2293
AflIII	ACRYGT	1 cut(s)	1424
AgsI	TTSAA	9 cut(s)	269, 424, 811, 929, 1006, 1223, 1328, 1865, 1886
AjnI	CCWGG	2 cut(s)	559, 703
Alw21I	GWGCWC	1 cut(s)	1476
Alw26I	GTCTC	2 cut(s)	52, 2003
AlwI	GGATC	1 cut(s)	1369
Ama87I	CYCGRG	2 cut(s)	92, 592
Aor13HI	TCCGGA	1 cut(s)	235
Aor51HI	AGCGCT	1 cut(s)	201
AoxI	GGCC	5 cut(s)	261, 351, 649, 662, 1278
ApeKI	GCWGC	5 cut(s)	440, 526, 986, 1530, 1702
ApoI	RAATTY	7 cut(s)	12, 239, 1241, 1384, 1518, 1568, 1975
Asp700I	GAANNNNTTC	2 cut(s)	273, 1925
Asp718I	GGTACC	1 cut(s)	1399
AspLEI	GCGC	3 cut(s)	170, 202, 349
AspS9I	GGNCC	3 cut(s)	649, 1639, 2084
AsuHPI	GGTGA	7 cut(s)	132, 217, 1403, 2094, 2122, 2175, 2272
AvaI	CYCGRG	2 cut(s)	92, 592
AvaII	GGWCC	2 cut(s)	1639, 2084
BalI	TGGCCA	1 cut(s)	353
BanI	GGYRCC	2 cut(s)	1399, 2003
BanII	GRGCYC	2 cut(s)	1355, 1476
BbsI	GAAGAC	3 cut(s)	365, 501, 1614
Bbv12I	GWGCWC	1 cut(s)	1476
BbvI	GCAGC	5 cut(s)	427, 513, 973, 1542, 1714
BccI	CCATC	7 cut(s)	213, 394, 409, 1081, 1327, 2107, 2236
BceAI	ACGGC	1 cut(s)	722
BcgI	CGANNNNNNTGC	4 cut(s)	848, 882, 921, 955
BciT130I	CCWGG	2 cut(s)	561, 705
BclI	TGATCA	1 cut(s)	1183
BcoDI	GTCTC	2 cut(s)	52, 2003
BfaI	CTAG	2 cut(s)	666, 894
BfmI	CTRYAG	2 cut(s)	732, 2172
BfoI	RGCGCY	2 cut(s)	203, 350
BglII	AGATCT	1 cut(s)	847
BisI	GCNGC	6 cut(s)	166, 441, 527, 987, 1531, 1703
BlpI	GCTNAGC	1 cut(s)	1830
BlsI	GCNGC	6 cut(s)	167, 442, 528, 988, 1532, 1704
Bme1390I	CCNGG	2 cut(s)	561, 705
Bme18I	GGWCC	2 cut(s)	1639, 2084
BmeT110I	CYCGRG	2 cut(s)	92, 592
BmgT120I	GGNCC	3 cut(s)	649, 1639, 2084
BmiI	GGNNCC	5 cut(s)	650, 695, 1401, 1577, 2005
BmrFI	CCNGG	2 cut(s)	561, 705
BmrI	ACTGGG	1 cut(s)	1926
BmsI	GCATC	7 cut(s)	294, 645, 1349, 1420, 1883, 2066, 2291
BmuI	ACTGGG	1 cut(s)	1926
BpiI	GAAGAC	3 cut(s)	365, 501, 1614
BpmI	CTGGAG	1 cut(s)	1180
Bpu1102I	GCTNAGC	1 cut(s)	1830
BpuEI	CTTGAG	2 cut(s)	145, 1400
Bsa29I	ATCGAT	1 cut(s)	1680
BsaHI	GRCGYC	1 cut(s)	539
BsaJI	CCNNGG	2 cut(s)	6, 703
BsaWI	WCCGGW	4 cut(s)	16, 235, 311, 362
Bsc4I	CCNNNNNNNGG	5 cut(s)	314, 318, 415, 2292, 2293
Bse118I	RCCGGY	1 cut(s)	343
Bse1I	ACTGG	4 cut(s)	74, 1921, 2013, 2166
Bse3DI	GCAATG	2 cut(s)	631, 1261
BseAI	TCCGGA	1 cut(s)	235
BseBI	CCWGG	2 cut(s)	561, 705
BseCI	ATCGAT	1 cut(s)	1680
BseDI	CCNNGG	2 cut(s)	6, 703
BseGI	GGATG	7 cut(s)	232, 763, 1092, 1364, 1441, 2228, 2282
BseLI	CCNNNNNNNGG	5 cut(s)	314, 318, 415, 2292, 2293
BseMI	GCAATG	2 cut(s)	631, 1261
BseMII	CTCAG	7 cut(s)	206, 1251, 1593, 1775, 1844, 1952, 2250
BseNI	ACTGG	4 cut(s)	74, 1921, 2013, 2166
BseRI	GAGGAG	3 cut(s)	706, 1484, 1525
BseXI	GCAGC	5 cut(s)	427, 513, 973, 1542, 1714
BsgI	GTGCAG	2 cut(s)	1728, 2096
Bsh1236I	CGCG	1 cut(s)	170
Bsh1285I	CGRYCG	1 cut(s)	1077
BshFI	GGCC	5 cut(s)	263, 353, 651, 664, 1280
BshNI	GGYRCC	2 cut(s)	1399, 2003
BshVI	ATCGAT	1 cut(s)	1680
BsiEI	CGRYCG	1 cut(s)	1077
BsiHKAI	GWGCWC	1 cut(s)	1476
BsiHKCI	CYCGRG	2 cut(s)	92, 592
BsiSI	CCGG	8 cut(s)	17, 236, 312, 344, 363, 542, 1363, 1495
BslI	CCNNNNNNNGG	5 cut(s)	314, 318, 415, 2292, 2293
BsmAI	GTCTC	2 cut(s)	52, 2003
BsmBI	CGTCTC	2 cut(s)	52, 2003
BsnI	GGCC	5 cut(s)	263, 353, 651, 664, 1280
BsoBI	CYCGRG	2 cut(s)	92, 592
Bsp1286I	GDGCHC	2 cut(s)	1355, 1476
Bsp13I	TCCGGA	1 cut(s)	235
Bsp143I	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
Bsp1720I	GCTNAGC	1 cut(s)	1830
BspACI	CCGC	2 cut(s)	165, 318
BspANI	GGCC	5 cut(s)	263, 353, 651, 664, 1280
BspCNI	CTCAG	7 cut(s)	207, 1252, 1594, 1776, 1843, 1951, 2249
BspDI	ATCGAT	1 cut(s)	1680
BspEI	TCCGGA	1 cut(s)	235
BspFNI	CGCG	1 cut(s)	170
BspLI	GGNNCC	5 cut(s)	650, 695, 1401, 1577, 2005
BspPI	GGATC	1 cut(s)	1369
BspT107I	GGYRCC	2 cut(s)	1399, 2003
BsrDI	GCAATG	2 cut(s)	631, 1261
BsrFI	RCCGGY	1 cut(s)	343
BsrI	ACTGG	4 cut(s)	74, 1921, 2013, 2166
BssAI	RCCGGY	1 cut(s)	343
BssECI	CCNNGG	2 cut(s)	6, 703
BssMI	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
BssNI	GRCGYC	1 cut(s)	539
Bst2UI	CCWGG	2 cut(s)	561, 705
Bst4CI	ACNGT	7 cut(s)	144, 516, 1075, 1413, 1580, 1954, 1972
Bst6I	CTCTTC	3 cut(s)	280, 646, 1290
BstACI	GRCGYC	1 cut(s)	539
BstAPI	GCANNNNNTGC	1 cut(s)	2054
BstDEI	CTNAG	8 cut(s)	215, 1250, 1260, 1602, 1784, 1830, 1938, 2236
BstF5I	GGATG	7 cut(s)	232, 763, 1092, 1364, 1441, 2228, 2282
BstFNI	CGCG	1 cut(s)	170
BstH2I	RGCGCY	2 cut(s)	203, 350
BstHHI	GCGC	3 cut(s)	170, 202, 349
BstKTI	GATC	8 cut(s)	850, 1186, 1377, 1558, 1670, 1680, 1996, 2276
BstMAI	GTCTC	2 cut(s)	52, 2003
BstMBI	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
BstMCI	CGRYCG	1 cut(s)	1077
BstNI	CCWGG	2 cut(s)	561, 705
BstNSI	RCATGY	1 cut(s)	1428
BstSCI	CCNGG	2 cut(s)	559, 703
BstSFI	CTRYAG	2 cut(s)	732, 2172
BstUI	CGCG	1 cut(s)	170
BstV1I	GCAGC	5 cut(s)	427, 513, 973, 1542, 1714
BstV2I	GAAGAC	3 cut(s)	365, 501, 1614
BstX2I	RGATCY	2 cut(s)	847, 1374
BstXI	CCANNNNNNTGG	2 cut(s)	1166, 1465
BstYI	RGATCY	2 cut(s)	847, 1374
Bsu15I	ATCGAT	1 cut(s)	1680
BsuRI	GGCC	5 cut(s)	263, 353, 651, 664, 1280
BsuTUI	ATCGAT	1 cut(s)	1680
BtgZI	GCGATG	1 cut(s)	957
BtsCI	GGATG	7 cut(s)	232, 763, 1092, 1364, 1441, 2228, 2282
BtsIMutI	CAGTG	3 cut(s)	81, 1409, 1959
CfoI	GCGC	3 cut(s)	170, 202, 349
Cfr10I	RCCGGY	1 cut(s)	343
Cfr13I	GGNCC	3 cut(s)	649, 1639, 2084
ClaI	ATCGAT	1 cut(s)	1680
CseI	GACGC	2 cut(s)	116, 547
Csp6I	GTAC	5 cut(s)	710, 884, 1400, 1950, 2208
CviAII	CATG	7 cut(s)	491, 645, 653, 981, 995, 1425, 2147
CviQI	GTAC	5 cut(s)	710, 884, 1400, 1950, 2208
DdeI	CTNAG	8 cut(s)	215, 1250, 1260, 1602, 1784, 1830, 1938, 2236
DpnI	GATC	8 cut(s)	849, 1185, 1376, 1557, 1669, 1679, 1995, 2275
DpnII	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
DraIII	CACNNNGTG	1 cut(s)	862
EaeI	YGGCCR	1 cut(s)	351
Eam1104I	CTCTTC	3 cut(s)	280, 646, 1290
EarI	CTCTTC	3 cut(s)	280, 646, 1290
Ecl136II	GAGCTC	1 cut(s)	1474
Eco147I	AGGCCT	2 cut(s)	664, 1280
Eco24I	GRGCYC	2 cut(s)	1355, 1476
Eco32I	GATATC	2 cut(s)	904, 978
Eco47I	GGWCC	2 cut(s)	1639, 2084
Eco47III	AGCGCT	1 cut(s)	201
Eco53kI	GAGCTC	1 cut(s)	1474
Eco88I	CYCGRG	2 cut(s)	92, 592
EcoICRI	GAGCTC	1 cut(s)	1474
EcoO109I	RGGNCCY	1 cut(s)	2084
EcoRI	GAATTC	2 cut(s)	239, 1568
EcoRII	CCWGG	2 cut(s)	559, 703
EcoRV	GATATC	2 cut(s)	904, 978
EcoT38I	GRGCYC	2 cut(s)	1355, 1476
Esp3I	CGTCTC	2 cut(s)	52, 2003
FaeI	CATG	7 cut(s)	494, 648, 656, 984, 998, 1428, 2150
FatI	CATG	7 cut(s)	490, 644, 652, 980, 994, 1424, 2146
FauI	CCCGC	1 cut(s)	311
FbaI	TGATCA	1 cut(s)	1183
FblI	GTMKAC	2 cut(s)	105, 918
Fnu4HI	GCNGC	6 cut(s)	166, 441, 527, 987, 1531, 1703
FokI	GGATG	7 cut(s)	219, 770, 1099, 1371, 1428, 2215, 2269
FriOI	GRGCYC	2 cut(s)	1355, 1476
Fsp4HI	GCNGC	6 cut(s)	166, 441, 527, 987, 1531, 1703
FspBI	CTAG	2 cut(s)	666, 894
GlaI	GCGC	3 cut(s)	169, 201, 348
GluI	GCNGC	6 cut(s)	166, 441, 527, 987, 1531, 1703
GsuI	CTGGAG	1 cut(s)	1180
HaeII	RGCGCY	2 cut(s)	203, 350
HaeIII	GGCC	5 cut(s)	263, 353, 651, 664, 1280
HapII	CCGG	8 cut(s)	17, 236, 312, 344, 363, 542, 1363, 1495
HgaI	GACGC	2 cut(s)	116, 547
HhaI	GCGC	3 cut(s)	170, 202, 349
Hin1I	GRCGYC	1 cut(s)	539
Hin1II	CATG	7 cut(s)	494, 648, 656, 984, 998, 1428, 2150
Hin6I	GCGC	3 cut(s)	168, 200, 347
HinP1I	GCGC	3 cut(s)	168, 200, 347
HincII	GTYRAC	1 cut(s)	106
HindII	GTYRAC	1 cut(s)	106
HindIII	AAGCTT	1 cut(s)	2166
HpaII	CCGG	8 cut(s)	17, 236, 312, 344, 363, 542, 1363, 1495
HphI	GGTGA	7 cut(s)	132, 217, 1403, 2094, 2122, 2175, 2272
Hpy166II	GTNNAC	6 cut(s)	22, 106, 710, 919, 2113, 2263
Hpy8I	GTNNAC	6 cut(s)	22, 106, 710, 919, 2113, 2263
Hpy99I	CGWCG	2 cut(s)	47, 110
HpyAV	CCTTC	5 cut(s)	418, 748, 1142, 2061, 2242
HpyCH4III	ACNGT	7 cut(s)	144, 516, 1075, 1413, 1580, 1954, 1972
HpyCH4IV	ACGT	1 cut(s)	882
HpyF3I	CTNAG	8 cut(s)	215, 1250, 1260, 1602, 1784, 1830, 1938, 2236
HpySE526I	ACGT	1 cut(s)	882
Hsp92I	GRCGYC	1 cut(s)	539
Hsp92II	CATG	7 cut(s)	494, 648, 656, 984, 998, 1428, 2150
HspAI	GCGC	3 cut(s)	168, 200, 347
Kpn2I	TCCGGA	1 cut(s)	235
KpnI	GGTACC	1 cut(s)	1403
Ksp22I	TGATCA	1 cut(s)	1183
Kzo9I	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
LmnI	GCTCC	4 cut(s)	44, 693, 1471, 1538
Lsp1109I	GCAGC	5 cut(s)	427, 513, 973, 1542, 1714
LweI	GCATC	7 cut(s)	294, 645, 1349, 1420, 1883, 2066, 2291
MaeI	CTAG	2 cut(s)	666, 894
MaeII	ACGT	1 cut(s)	882
MaeIII	GTNAC	7 cut(s)	76, 535, 1405, 1633, 1895, 2181, 2325
MalI	GATC	8 cut(s)	849, 1185, 1376, 1557, 1669, 1679, 1995, 2275
MboI	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
MfeI	CAATTG	3 cut(s)	254, 912, 2063
MflI	RGATCY	2 cut(s)	847, 1374
MhlI	GDGCHC	2 cut(s)	1355, 1476
MlsI	TGGCCA	1 cut(s)	353
MluNI	TGGCCA	1 cut(s)	353
MlyI	GAGTC	2 cut(s)	776, 1172
Mox20I	TGGCCA	1 cut(s)	353
MroI	TCCGGA	1 cut(s)	235
MroXI	GAANNNNTTC	2 cut(s)	273, 1925
MscI	TGGCCA	1 cut(s)	353
MseI	TTAA	7 cut(s)	605, 1128, 1548, 1758, 1850, 1988, 2298
MslI	CAYNNNNRTG	1 cut(s)	2151
Msp20I	TGGCCA	1 cut(s)	353
MspI	CCGG	8 cut(s)	17, 236, 312, 344, 363, 542, 1363, 1495
MspR9I	CCNGG	2 cut(s)	561, 705
MunI	CAATTG	3 cut(s)	254, 912, 2063
MvaI	CCWGG	2 cut(s)	561, 705
MvnI	CGCG	1 cut(s)	170
NdeII	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
NlaIII	CATG	7 cut(s)	494, 648, 656, 984, 998, 1428, 2150
NlaIV	GGNNCC	5 cut(s)	650, 695, 1401, 1577, 2005
NmeAIII	GCCGAG	2 cut(s)	1850, 1980
NmuCI	GTSAC	3 cut(s)	76, 535, 2181
NspI	RCATGY	1 cut(s)	1428
PaeR7I	CTCGAG	2 cut(s)	92, 592
PceI	AGGCCT	2 cut(s)	664, 1280
PciI	ACATGT	1 cut(s)	1424
PdmI	GAANNNNTTC	2 cut(s)	273, 1925
PfeI	GAWTC	8 cut(s)	26, 178, 366, 503, 586, 1619, 2198, 2305
PflMI	CCANNNNNTGG	1 cut(s)	415
PkrI	GCNGC	6 cut(s)	167, 442, 528, 988, 1532, 1704
PleI	GAGTC	2 cut(s)	775, 1171
PpsI	GAGTC	2 cut(s)	775, 1171
PpuMI	RGGWCCY	1 cut(s)	2084
PscI	ACATGT	1 cut(s)	1424
Psp124BI	GAGCTC	1 cut(s)	1476
Psp5II	RGGWCCY	1 cut(s)	2084
Psp6I	CCWGG	2 cut(s)	559, 703
PspGI	CCWGG	2 cut(s)	559, 703
PspN4I	GGNNCC	5 cut(s)	650, 695, 1401, 1577, 2005
PspPI	GGNCC	3 cut(s)	649, 1639, 2084
PspPPI	RGGWCCY	1 cut(s)	2084
PspXI	VCTCGAGB	1 cut(s)	92
PsuI	RGATCY	2 cut(s)	847, 1374
RsaI	GTAC	5 cut(s)	711, 885, 1401, 1951, 2209
RsaNI	GTAC	5 cut(s)	710, 884, 1400, 1950, 2208
RseI	CAYNNNNRTG	1 cut(s)	2151
SacI	GAGCTC	1 cut(s)	1476
SalI	GTCGAC	1 cut(s)	104
SaqAI	TTAA	7 cut(s)	605, 1128, 1548, 1758, 1850, 1988, 2298
SatI	GCNGC	6 cut(s)	166, 441, 527, 987, 1531, 1703
Sau3AI	GATC	8 cut(s)	847, 1183, 1374, 1555, 1667, 1677, 1993, 2273
Sau96I	GGNCC	3 cut(s)	649, 1639, 2084
SchI	GAGTC	2 cut(s)	776, 1172
ScrFI	CCNGG	2 cut(s)	561, 705
SduI	GDGCHC	2 cut(s)	1355, 1476
SfaNI	GCATC	7 cut(s)	294, 645, 1349, 1420, 1883, 2066, 2291
SfcI	CTRYAG	2 cut(s)	732, 2172
Sfr274I	CTCGAG	2 cut(s)	92, 592
SinI	GGWCC	2 cut(s)	1639, 2084
SlaI	CTCGAG	2 cut(s)	92, 592
SmiMI	CAYNNNNRTG	1 cut(s)	2151
SmlI	CTYRAG	4 cut(s)	92, 124, 592, 1379
SmoI	CTYRAG	4 cut(s)	92, 124, 592, 1379
SseBI	AGGCCT	2 cut(s)	664, 1280
SsiI	CCGC	2 cut(s)	165, 318
SspI	AATATT	2 cut(s)	1132, 1721
SspMI	CTAG	2 cut(s)	666, 894
SstI	GAGCTC	1 cut(s)	1476
StuI	AGGCCT	2 cut(s)	664, 1280
StyD4I	CCNGG	2 cut(s)	559, 703
TaaI	ACNGT	7 cut(s)	144, 516, 1075, 1413, 1580, 1954, 1972
TaiI	ACGT	1 cut(s)	885
TaqI	TCGA	8 cut(s)	93, 105, 326, 593, 673, 838, 1516, 1680
TaqII	GACCGA	2 cut(s)	218, 1334
TatI	WGTACW	2 cut(s)	1949, 2207
TauI	GCSGC	1 cut(s)	168
TfiI	GAWTC	8 cut(s)	26, 178, 366, 503, 586, 1619, 2198, 2305
Tru1I	TTAA	7 cut(s)	605, 1128, 1548, 1758, 1850, 1988, 2298
Tru9I	TTAA	7 cut(s)	605, 1128, 1548, 1758, 1850, 1988, 2298
TscAI	CASTG	3 cut(s)	81, 1416, 1959
TseFI	GTSAC	3 cut(s)	76, 535, 2181
TseI	GCWGC	5 cut(s)	440, 526, 986, 1530, 1702
Tsp45I	GTSAC	3 cut(s)	76, 535, 2181
TspGWI	ACGGA	3 cut(s)	31, 1652, 1763
TspRI	CASTG	3 cut(s)	81, 1416, 1959
Van91I	CCANNNNNTGG	1 cut(s)	415
VpaK11BI	GGWCC	2 cut(s)	1639, 2084
XapI	RAATTY	7 cut(s)	12, 239, 1241, 1384, 1518, 1568, 1975
XceI	RCATGY	1 cut(s)	1428
XhoI	CTCGAG	2 cut(s)	92, 592
XmiI	GTMKAC	2 cut(s)	105, 918
XmnI	GAANNNNTTC	2 cut(s)	273, 1925
XspI	CTAG	2 cut(s)	666, 894

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.