FvH4_1g09400

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0007042
Physical Location & Seq
Reverse (-)
5042739 .. 5051506
8768 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g09400.t2

Sequence Viewer

Length: 2541 bp
ATGGCGTCGTCGGAAAATGAGCCGGAGCAGCCGCCGTCCTCCCGCGCCGGAGGAGACGAAGAAGAAGCTATTACTCCTCCTCCTAAGCACGTTGATATGCAGAAATTCAGGCTTTACGAGACTCGATCGAACTTTTACATGATTGGGAGAGACAAGAGTAGAACGTATTGGAGGGTACTGAAGATTGATAGATTGGATCCTTGTGAGCTTAATGTTCTTGAAGATTCTACTACTTATACCGAGCGTGAATGTTCTGACCTCTTGAGGCGGGTACATGAAGGGAACAAGGTCACTGGTGGATTGAAGTTTGTCACTACTTGTTACGGCATTATTGGGTTCATTAAGTTTCTGGGGCCGTATTACATGCTTCTTATAACAAAAAGAAGGAAGATAGGTGACATTCGTGGCCACGCGGTGTATGGTATCTCCAAAAGTGAAATGATCCCGCTTCCAAATTCTTCTGTTCAGTGCAGTATGTCTGATTCTAGAGATGAAAACAGATACAAGAAGCTCTTGTGCACAATGGACCTCACAAAGGACTTCTTTTTCAGCTACTCATACAATGTCATGCGTAGTCTACAGAAGAACATGTTGGATAATAAGATGGGACATGTCGTGTATGAAACCATGTTTGTGTGGAATGAGTTCTTAACTCGGGGGATAAGGAATCACCTGCAAAATACTGTTTGGACAGTTGCATTGGTTTATGGTTTCTTTAGACAGGCCACTCTCTCTACATCTGGACGTGACTTCAAACTGACCCTCATTGCAAGACGTTCACGTCACTATGCGGGAACCAGGTATCTAAAACGTGGTGTTAATGAGAAGGGAAGAGTGGCTAATGATGTTGAGACCGAACAAATAGTTTTTGAGGATGTTCCTGAGGGCTTCCCCATTCAAATAAGTTCTGTTGTACAGAACCGTGGCTCAATCCCTCTCTATTGGTCACAAGAAACATCGCGTTTGAACATCAAACCAGATATAATATTGTCAAAGAAGGATCAAAACTACGAAGCCACTAGGCTTCATTTTGAAAATCTTGTCAAGAGATATGGGAACCCCATTATCATTTTAAATCTCATCAAGACACAGGAAAAGAGGCCTCGAGAGTCCATTCTACGCACAGAGTTTGCTAATGCAATTGATTTCATAAATAAAGATCTATCTGAGGAGAACCGTCTCAGATTCCTCCACTGGGATTTGCACAAACATTCTCGAAGCAAAGCTGTAAATGCGCTACTACTCTTGGGCAAGGTGGCTACATATGCCTTGAGTTTAACTGGATTCTTCCATTGTCAAGTTACACCAGAAATGGGACCTGAGGGCTTGATTAATTGCCCTTCATCTGAGGATTTCGACAATTGTACATCTCCCCAAAGACATTATGCAAATGGAGACGAGGATGTTGATAACATAGCGGGGAAACCAACTGGAGACAGCAATGTTGGAAATGGAGGTCATTCTGCCAAACTATCCACATTCCAGAAGGGGGTGCTGAGGACCAACTGCATAGACTGCCTTGATCGGACTAATGTTGCGCAGTATGCATATGGGTTGGCTGCTCTAGGACACCAGCTTCATGCTTTGGGACTTTTAGAGAATCCCAAGATAGACCTTGACGATCCCTTGGCTGATGAATTGATGGGATTTTATGAAAGAATGGGTGATACACTTGCCCACCAGTATGGTGGTTCAGCTGCTCACAATAAGATATTTTCCGAGAGAAGAGGTCAATGGAGAGCAGCAACTCAGTCTCAGGAATTCTTCAGAACTCTACAACGGTATTACAGCAATGCGTATATGGATGCACAGAAGCAAGATGCAATTAATATATTTCTCGGGGATTTTCAACCGCAGCAAGATAAACCTGCACTCTGGGAACTGGATTTAGACCAGCATTACCATGATGGGAGGCATGGGTCAAAAGACATGGATGAAGATGGCTGGTCTACAATCAAGAGATCATATTCCGACGGAAACATTCTTCGTCCAAATAGCTCACCTATGTCAGCACCAAGTGTGAAGCAGGAGACTACCTATCCAGTTTTGCCTAATCAAACTAAACAAAGCAAGATACTTTCAGAATCGTCGCCTGAAATATCAACTTGTGAGAGCGATATTGCATATTCAAGGTACACTCCCTCAATGCCTCGCAGACAACTTTTTGGAGAGTTGCAAAGATATCGGTGTCTTGAAAGTGATCATATTTATTATTCCGAACGTGGAGATGGATATAATTTCTCGAACTTTGTGGATCTAGACTGGCTATCTTCCTCTGGGAATTCATGTGAGGAGGAGCCGTTTGAGAGATCGTCATTACTTACCAACTCACCCCTTGAGGGGCTATCATCGGAGAATGTTGTCAACGGAATTATAGGAGAGTCTACACCCTCCACAAGTGAATATGGATCCATGTTGAAGGGAAAGGGGCAGACAGGAATAGGATCATCCTTCACTAATGGAGAGAGTTCCAATGTTCCTGAAGACTTCCCAGATAGCTTTGCGAACTGGGTCAATTATGGAGAGACACTATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005811 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0009506 GO:0009653 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031982 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034596 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098805 GO:0120035 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

847

Amino Acids

96.22

Weight (kDa)

5.99

Isoelectric Point (pI)

51.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 108 - 406 6.4e-84 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 374
AarI CACCTGC 1 cut(s) 681
AasI GACNNNNNNGTC 1 cut(s) 780
Acc16I TGCGCA 1 cut(s) 1539
Acc36I ACCTGC 2 cut(s) 681, 1876
AccI GTMKAC 3 cut(s) 577, 1949, 2384
AccII CGCG 3 cut(s) 45, 413, 961
AciI CCGC 8 cut(s) 32, 43, 268, 413, 446, 791, 1418, 1853
AclWI GGATC 9 cut(s) 191, 204, 436, 1008, 1616, 2262, 2403, 2416, 2452
AcoI YGGCCR 1 cut(s) 406
AcsI RAATTY 4 cut(s) 104, 454, 1760, 2281
AcuI CTGAAG 3 cut(s) 200, 1750, 2502
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 2 cut(s) 415, 2018
AfaI GTAC 5 cut(s) 177, 273, 915, 1366, 2135
AfiI CCNNNNNNNGG 6 cut(s) 535, 1195, 1313, 1489, 2339, 2340
AflIII ACRYGT 2 cut(s) 588, 610
AjiI CACGTC 2 cut(s) 746, 782
AjnI CCWGG 1 cut(s) 797
AjuI GAANNNNNNNTTGG 2 cut(s) 575, 607
AluBI AGCT 9 cut(s) 68, 208, 511, 552, 1226, 1576, 1697, 1998, 2499
AluI AGCT 9 cut(s) 68, 208, 511, 552, 1226, 1576, 1697, 1998, 2499
Alw21I GWGCWC 1 cut(s) 521
Alw44I GTGCAC 1 cut(s) 517
AlwI GGATC 9 cut(s) 191, 204, 436, 1008, 1616, 2262, 2403, 2416, 2452
Ama87I CYCGRG 3 cut(s) 654, 1104, 1838
AoxI GGCC 4 cut(s) 353, 406, 723, 1100
ApaLI GTGCAC 1 cut(s) 517
ApeKI GCWGC 5 cut(s) 28, 1559, 1697, 1742, 1855
ApoI RAATTY 4 cut(s) 104, 454, 1760, 2281
AseI ATTAAT 2 cut(s) 1332, 1827
Asp700I GAANNNNTTC 1 cut(s) 644
AspLEI GCGC 3 cut(s) 47, 1237, 1540
AspS9I GGNCC 4 cut(s) 353, 526, 1316, 1500
AsuHPI GGTGA 5 cut(s) 407, 662, 1676, 1992, 2322
AvaI CYCGRG 3 cut(s) 654, 1104, 1838
AvaII GGWCC 3 cut(s) 526, 1316, 1500
AxyI CCTNAGG 2 cut(s) 882, 1320
BaeGI GKGCMC 1 cut(s) 521
BalI TGGCCA 1 cut(s) 408
BamHI GGATCC 2 cut(s) 196, 2408
BbsI GAAGAC 1 cut(s) 2490
Bbv12I GWGCWC 1 cut(s) 521
BbvCI CCTCAGC 1 cut(s) 1496
BbvI GCAGC 5 cut(s) 40, 1546, 1684, 1754, 1867
BccI CCATC 5 cut(s) 598, 1636, 1901, 1934, 2222
BceAI ACGGC 4 cut(s) 19, 340, 340, 2284
BcgI CGANNNNNNTGC 2 cut(s) 2165, 2199
BciT130I CCWGG 1 cut(s) 799
BclI TGATCA 1 cut(s) 2200
BfaI CTAG 4 cut(s) 486, 1020, 1565, 2258
BfmI CTRYAG 1 cut(s) 578
BfuAI ACCTGC 2 cut(s) 681, 1876
BglII AGATCT 1 cut(s) 1159
BisI GCNGC 6 cut(s) 29, 32, 1560, 1698, 1743, 1856
BlsI GCNGC 6 cut(s) 30, 33, 1561, 1699, 1744, 1857
Bme1390I CCNGG 1 cut(s) 799
Bme18I GGWCC 3 cut(s) 526, 1316, 1500
BmeT110I CYCGRG 3 cut(s) 654, 1104, 1838
BmgBI CACGTC 2 cut(s) 746, 782
BmgT120I GGNCC 4 cut(s) 353, 526, 1316, 1500
BmiI GGNNCC 7 cut(s) 198, 354, 796, 1058, 1317, 2298, 2410
BmrFI CCNGG 1 cut(s) 799
BmrI ACTGGG 2 cut(s) 1204, 2518
BmsI GCATC 2 cut(s) 1795, 1810
BmuI ACTGGG 2 cut(s) 1204, 2518
BpiI GAAGAC 1 cut(s) 2490
BpmI CTGGAG 1 cut(s) 1452
Bpu10I CCTNAGC 2 cut(s) 84, 1496
BpuEI CTTGAG 3 cut(s) 283, 1291, 2357
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 845
BsaJI CCNNGG 2 cut(s) 922, 1626
BsaXI ACNNNNNCTCC 4 cut(s) 64, 94, 2285, 2315
Bsc4I CCNNNNNNNGG 6 cut(s) 535, 1195, 1313, 1489, 2339, 2340
Bse1I ACTGG 9 cut(s) 298, 1199, 1285, 1435, 1681, 1887, 2042, 2267, 2513
Bse21I CCTNAGG 2 cut(s) 882, 1320
Bse3DI GCAATG 3 cut(s) 765, 1447, 1798
BseBI CCWGG 1 cut(s) 799
BseDI CCNNGG 2 cut(s) 922, 1626
BseGI GGATG 5 cut(s) 880, 1408, 1810, 1939, 2447
BseLI CCNNNNNNNGG 6 cut(s) 535, 1195, 1313, 1489, 2339, 2340
BseMI GCAATG 3 cut(s) 765, 1447, 1798
BseMII CTCAG 8 cut(s) 873, 1158, 1195, 1311, 1338, 1487, 1763, 1769
BseNI ACTGG 9 cut(s) 298, 1199, 1285, 1435, 1681, 1887, 2042, 2267, 2513
BseRI GAGGAG 6 cut(s) 66, 66, 69, 1184, 2306, 2309
BseSI GKGCMC 1 cut(s) 521
BseXI GCAGC 5 cut(s) 40, 1546, 1684, 1754, 1867
BsgI GTGCAG 2 cut(s) 490, 1854
Bsh1236I CGCG 3 cut(s) 45, 413, 961
Bsh1285I CGRYCG 1 cut(s) 128
BshFI GGCC 4 cut(s) 355, 408, 725, 1102
BsiEI CGRYCG 1 cut(s) 128
BsiHKAI GWGCWC 1 cut(s) 521
BsiHKCI CYCGRG 3 cut(s) 654, 1104, 1838
BsiSI CCGG 2 cut(s) 23, 48
BslFI GGGAC 3 cut(s) 621, 1329, 1602
BslI CCNNNNNNNGG 6 cut(s) 535, 1195, 1313, 1489, 2339, 2340
BsmBI CGTCTC 3 cut(s) 48, 1184, 1389
BsmFI GGGAC 3 cut(s) 621, 1329, 1602
BsnI GGCC 4 cut(s) 355, 408, 725, 1102
Bso31I GGTCTC 1 cut(s) 845
BsoBI CYCGRG 3 cut(s) 654, 1104, 1838
Bsp1286I GDGCHC 1 cut(s) 521
Bsp1407I TGTACA 2 cut(s) 913, 1364
BspACI CCGC 8 cut(s) 32, 43, 268, 413, 446, 791, 1418, 1853
BspANI GGCC 4 cut(s) 355, 408, 725, 1102
BspCNI CTCAG 8 cut(s) 874, 1159, 1194, 1312, 1339, 1488, 1762, 1768
BspFNI CGCG 3 cut(s) 45, 413, 961
BspLI GGNNCC 7 cut(s) 198, 354, 796, 1058, 1317, 2298, 2410
BspMI ACCTGC 2 cut(s) 681, 1876
BspPI GGATC 9 cut(s) 191, 204, 436, 1008, 1616, 2262, 2403, 2416, 2452
BspTNI GGTCTC 1 cut(s) 845
BsrDI GCAATG 3 cut(s) 765, 1447, 1798
BsrGI TGTACA 2 cut(s) 913, 1364
BsrI ACTGG 9 cut(s) 298, 1199, 1285, 1435, 1681, 1887, 2042, 2267, 2513
BssECI CCNNGG 2 cut(s) 922, 1626
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1626
Bst2UI CCWGG 1 cut(s) 799
Bst4CI ACNGT 5 cut(s) 685, 694, 923, 1178, 1782
Bst6I CTCTTC 2 cut(s) 826, 1720
BstACI GRCGYC 1 cut(s) 5
BstAPI GCANNNNNTGC 1 cut(s) 1515
BstAUI TGTACA 2 cut(s) 913, 1364
BstDEI CTNAG 9 cut(s) 84, 882, 1167, 1181, 1320, 1347, 1496, 1749, 1755
BstDSI CCRYGG 1 cut(s) 922
BstENI CCTNNNNNAGG 1 cut(s) 533
BstF5I GGATG 5 cut(s) 880, 1408, 1810, 1939, 2447
BstFNI CGCG 3 cut(s) 45, 413, 961
BstHHI GCGC 3 cut(s) 47, 1237, 1540
BstMCI CGRYCG 1 cut(s) 128
BstMWI GCNNNNNNNGC 5 cut(s) 28, 1232, 1265, 1515, 1544
BstNI CCWGG 1 cut(s) 799
BstNSI RCATGY 3 cut(s) 367, 592, 614
BstSCI CCNGG 1 cut(s) 797
BstSFI CTRYAG 1 cut(s) 578
BstSLI GKGCMC 1 cut(s) 521
BstUI CGCG 3 cut(s) 45, 413, 961
BstV1I GCAGC 5 cut(s) 40, 1546, 1684, 1754, 1867
BstV2I GAAGAC 1 cut(s) 2490
BstX2I RGATCY 4 cut(s) 196, 1159, 2254, 2408
BstXI CCANNNNNNTGG 2 cut(s) 1685, 1688
BstYI RGATCY 4 cut(s) 196, 1159, 2254, 2408
Bsu36I CCTNAGG 2 cut(s) 882, 1320
BsuRI GGCC 4 cut(s) 355, 408, 725, 1102
BtgI CCRYGG 1 cut(s) 922
BtgZI GCGATG 1 cut(s) 942
BtrI CACGTC 2 cut(s) 746, 782
BtsCI GGATG 5 cut(s) 880, 1408, 1810, 1939, 2447
BtsIMutI CAGTG 4 cut(s) 291, 473, 1192, 2536
BveI ACCTGC 2 cut(s) 681, 1876
CfoI GCGC 3 cut(s) 47, 1237, 1540
Cfr13I GGNCC 4 cut(s) 353, 526, 1316, 1500
CsiI ACCWGGT 1 cut(s) 797
Csp6I GTAC 5 cut(s) 176, 272, 914, 1365, 2134
CviQI GTAC 5 cut(s) 176, 272, 914, 1365, 2134
DdeI CTNAG 9 cut(s) 84, 882, 1167, 1181, 1320, 1347, 1496, 1749, 1755
DraI TTTAAA 1 cut(s) 1074
DraIII CACNNNGTG 2 cut(s) 415, 2018
DrdI GACNNNNNNGTC 1 cut(s) 780
DseDI GACNNNNNNGTC 1 cut(s) 780
EaeI YGGCCR 1 cut(s) 406
Eam1104I CTCTTC 2 cut(s) 826, 1720
EarI CTCTTC 2 cut(s) 826, 1720
Eco130I CCWWGG 1 cut(s) 1626
Eco147I AGGCCT 1 cut(s) 1102
Eco31I GGTCTC 1 cut(s) 845
Eco32I GATATC 1 cut(s) 2183
Eco47I GGWCC 3 cut(s) 526, 1316, 1500
Eco57I CTGAAG 3 cut(s) 200, 1750, 2502
Eco81I CCTNAGG 2 cut(s) 882, 1320
Eco88I CYCGRG 3 cut(s) 654, 1104, 1838
EcoNI CCTNNNNNAGG 1 cut(s) 533
EcoO109I RGGNCCY 1 cut(s) 1316
EcoRI GAATTC 2 cut(s) 1760, 2281
EcoRII CCWGG 1 cut(s) 797
EcoRV GATATC 1 cut(s) 2183
EcoT14I CCWWGG 1 cut(s) 1626
EcoT22I ATGCAT 1 cut(s) 1549
ErhI CCWWGG 1 cut(s) 1626
Esp3I CGTCTC 3 cut(s) 48, 1184, 1389
FalI AAGNNNNNCTT 4 cut(s) 497, 529, 527, 559
FaqI GGGAC 3 cut(s) 621, 1329, 1602
FauI CCCGC 5 cut(s) 50, 261, 453, 784, 1411
FauNDI CATATG 2 cut(s) 1264, 1549
FbaI TGATCA 1 cut(s) 2200
FblI GTMKAC 3 cut(s) 577, 1949, 2384
Fnu4HI GCNGC 6 cut(s) 29, 32, 1560, 1698, 1743, 1856
FokI GGATG 5 cut(s) 887, 1415, 1817, 1946, 2434
Fsp4HI GCNGC 6 cut(s) 29, 32, 1560, 1698, 1743, 1856
FspBI CTAG 4 cut(s) 486, 1020, 1565, 2258
FspI TGCGCA 1 cut(s) 1539
GlaI GCGC 3 cut(s) 46, 1236, 1539
GluI GCNGC 6 cut(s) 29, 32, 1560, 1698, 1743, 1856
GsuI CTGGAG 1 cut(s) 1452
HaeIII GGCC 4 cut(s) 355, 408, 725, 1102
HapII CCGG 2 cut(s) 23, 48
HhaI GCGC 3 cut(s) 47, 1237, 1540
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 3 cut(s) 45, 1235, 1538
HinP1I GCGC 3 cut(s) 45, 1235, 1538
HincII GTYRAC 1 cut(s) 2365
HindII GTYRAC 1 cut(s) 2365
HpaII CCGG 2 cut(s) 23, 48
HphI GGTGA 5 cut(s) 407, 662, 1676, 1992, 2322
Hpy166II GTNNAC 7 cut(s) 519, 578, 779, 1950, 2136, 2365, 2385
Hpy8I GTNNAC 7 cut(s) 519, 578, 779, 1950, 2136, 2365, 2385
Hpy99I CGWCG 4 cut(s) 10, 13, 1976, 2092
HpyAV CCTTC 8 cut(s) 272, 378, 820, 991, 1350, 1480, 2413, 2461
HpyCH4III ACNGT 5 cut(s) 685, 694, 923, 1178, 1782
HpyCH4IV ACGT 7 cut(s) 90, 164, 745, 775, 781, 811, 2221
HpyF10VI GCNNNNNNNGC 5 cut(s) 28, 1232, 1265, 1515, 1544
HpyF3I CTNAG 9 cut(s) 84, 882, 1167, 1181, 1320, 1347, 1496, 1749, 1755
HpySE526I ACGT 7 cut(s) 90, 164, 745, 775, 781, 811, 2221
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 3 cut(s) 45, 1235, 1538
Ksp22I TGATCA 1 cut(s) 2200
LmnI GCTCC 2 cut(s) 25, 2296
Lsp1109I GCAGC 5 cut(s) 40, 1546, 1684, 1754, 1867
LweI GCATC 2 cut(s) 1795, 1810
MabI ACCWGGT 1 cut(s) 797
MaeI CTAG 4 cut(s) 486, 1020, 1565, 2258
MaeII ACGT 7 cut(s) 90, 164, 745, 775, 781, 811, 2221
MaeIII GTNAC 8 cut(s) 289, 310, 320, 395, 746, 782, 945, 1300
MfeI CAATTG 2 cut(s) 1140, 1360
MflI RGATCY 4 cut(s) 196, 1159, 2254, 2408
MhlI GDGCHC 1 cut(s) 521
MlsI TGGCCA 1 cut(s) 408
MluNI TGGCCA 1 cut(s) 408
MlyI GAGTC 3 cut(s) 115, 1118, 2390
MmeI TCCRAC 3 cut(s) 573, 1426, 1995
Mox20I TGGCCA 1 cut(s) 408
Mph1103I ATGCAT 1 cut(s) 1549
MroXI GAANNNNTTC 1 cut(s) 644
MscI TGGCCA 1 cut(s) 408
MseI TTAA 8 cut(s) 210, 342, 650, 819, 1073, 1277, 1332, 1827
MslI CAYNNNNRTG 3 cut(s) 632, 1683, 1902
Msp20I TGGCCA 1 cut(s) 408
MspA1I CMGCKG 1 cut(s) 1697
MspI CCGG 2 cut(s) 23, 48
MspR9I CCNGG 1 cut(s) 799
MunI CAATTG 2 cut(s) 1140, 1360
MvaI CCWGG 1 cut(s) 799
MvnI CGCG 3 cut(s) 45, 413, 961
MwoI GCNNNNNNNGC 5 cut(s) 28, 1232, 1265, 1515, 1544
NdeI CATATG 2 cut(s) 1264, 1549
NlaIV GGNNCC 7 cut(s) 198, 354, 796, 1058, 1317, 2298, 2410
NmuCI GTSAC 6 cut(s) 289, 310, 395, 746, 782, 945
NsbI TGCGCA 1 cut(s) 1539
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 3 cut(s) 367, 592, 614
PaeR7I CTCGAG 1 cut(s) 1104
PaqCI CACCTGC 1 cut(s) 681
PceI AGGCCT 1 cut(s) 1102
PciI ACATGT 2 cut(s) 588, 610
PdmI GAANNNNTTC 1 cut(s) 644
PfeI GAWTC 7 cut(s) 224, 482, 667, 1185, 1284, 1600, 2084
PkrI GCNGC 6 cut(s) 30, 33, 1561, 1699, 1744, 1857
Ple19I CGATCG 1 cut(s) 128
PleI GAGTC 3 cut(s) 115, 1117, 2389
PpsI GAGTC 3 cut(s) 115, 1117, 2389
PpuMI RGGWCCY 1 cut(s) 1316
PscI ACATGT 2 cut(s) 588, 610
PshBI ATTAAT 2 cut(s) 1332, 1827
PsiI TTATAA 1 cut(s) 374
Psp5II RGGWCCY 1 cut(s) 1316
Psp6I CCWGG 1 cut(s) 797
PspGI CCWGG 1 cut(s) 797
PspN4I GGNNCC 7 cut(s) 198, 354, 796, 1058, 1317, 2298, 2410
PspPI GGNCC 4 cut(s) 353, 526, 1316, 1500
PspPPI RGGWCCY 1 cut(s) 1316
PsuI RGATCY 4 cut(s) 196, 1159, 2254, 2408
PvuI CGATCG 1 cut(s) 128
PvuII CAGCTG 1 cut(s) 1697
RsaI GTAC 5 cut(s) 177, 273, 915, 1366, 2135
RsaNI GTAC 5 cut(s) 176, 272, 914, 1365, 2134
RseI CAYNNNNRTG 3 cut(s) 632, 1683, 1902
SaqAI TTAA 8 cut(s) 210, 342, 650, 819, 1073, 1277, 1332, 1827
SatI GCNGC 6 cut(s) 29, 32, 1560, 1698, 1743, 1856
Sau96I GGNCC 4 cut(s) 353, 526, 1316, 1500
SchI GAGTC 3 cut(s) 115, 1118, 2390
ScrFI CCNGG 1 cut(s) 799
SduI GDGCHC 1 cut(s) 521
SexAI ACCWGGT 1 cut(s) 797
SfaNI GCATC 2 cut(s) 1795, 1810
SfcI CTRYAG 1 cut(s) 578
Sfr274I CTCGAG 1 cut(s) 1104
SinI GGWCC 3 cut(s) 526, 1316, 1500
SlaI CTCGAG 1 cut(s) 1104
SmiMI CAYNNNNRTG 3 cut(s) 632, 1683, 1902
SmlI CTYRAG 4 cut(s) 262, 1104, 1270, 2336
SmoI CTYRAG 4 cut(s) 262, 1104, 1270, 2336
SseBI AGGCCT 1 cut(s) 1102
SsiI CCGC 8 cut(s) 32, 43, 268, 413, 446, 791, 1418, 1853
SspI AATATT 1 cut(s) 987
SspMI CTAG 4 cut(s) 486, 1020, 1565, 2258
StuI AGGCCT 1 cut(s) 1102
StyD4I CCNGG 1 cut(s) 797
StyI CCWWGG 1 cut(s) 1626
TaaI ACNGT 5 cut(s) 685, 694, 923, 1178, 1782
TaiI ACGT 7 cut(s) 93, 167, 748, 778, 784, 814, 2224
TaqI TCGA 6 cut(s) 124, 128, 1105, 1216, 1356, 2243
TaqII GACCGA 1 cut(s) 869
TatI WGTACW 2 cut(s) 913, 1364
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 7 cut(s) 224, 482, 667, 1185, 1284, 1600, 2084
Tru1I TTAA 8 cut(s) 210, 342, 650, 819, 1073, 1277, 1332, 1827
Tru9I TTAA 8 cut(s) 210, 342, 650, 819, 1073, 1277, 1332, 1827
TscAI CASTG 3 cut(s) 298, 473, 1199
TseFI GTSAC 6 cut(s) 289, 310, 395, 746, 782, 945
TseI GCWGC 5 cut(s) 28, 1559, 1697, 1742, 1855
Tsp45I GTSAC 6 cut(s) 289, 310, 395, 746, 782, 945
TspGWI ACGGA 2 cut(s) 1989, 2382
TspRI CASTG 3 cut(s) 298, 473, 1199
VneI GTGCAC 1 cut(s) 517
VpaK11BI GGWCC 3 cut(s) 526, 1316, 1500
VspI ATTAAT 2 cut(s) 1332, 1827
XagI CCTNNNNNAGG 1 cut(s) 533
XapI RAATTY 4 cut(s) 104, 454, 1760, 2281
XbaI TCTAGA 2 cut(s) 485, 2257
XceI RCATGY 3 cut(s) 367, 592, 614
XcmI CCANNNNNNNNNTGG 2 cut(s) 416, 1685
XhoI CTCGAG 1 cut(s) 1104
XmiI GTMKAC 3 cut(s) 577, 1949, 2384
XmnI GAANNNNTTC 1 cut(s) 644
XspI CTAG 4 cut(s) 486, 1020, 1565, 2258
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.