Rroxscaffold_2G00145930

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007042
Physical Location & Seq
Forward (+)
83787405 .. 83796158
8754 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00145930.1

Sequence Viewer

Length: 2451 bp
ATGGCCTCGTCGGAAAATGCGCCGGAGCCGCCGCCGTCCTCCCACGCCGGAGGAGACGAGGAAGTTATTATTACTCCTCCCTCAGCACATAGATACATAGATATGCAGAAATTCAGGCTTTACGAGACTCGATCGAACTTTTACATGATTGGAAGGGACAAGAGTAGAACGTATTGGAGGGTACTAAAGATTGATCGATTGGATCCCTGTGAGCTCAATGTTCTTGAAGATTCTACTACTTATACGGAACGTGAATGTTCTGACCTCTTGAGGCGGGTACATGAAGGGAACAAGGTCACTGGTGGATTGAAGTTTGTCACTACTTGTTACGGCATTATTGGGTTCATTAAATTTCTGGGCCCCTATTACATGCTGCTTATAACAAAGAGAAGAAAGATAGGTGCCATTCGTGGCCACGCGGTGTATGCCATCTCCAAAAGTGAAATGATCCCACTTCCAAATTCACCTGTTCAGTGCAGTATCGCTGATTGTAGAGATGAAAACAGATACAAGAAGCTCTTGTGCACAATGGACCTCACAAAGGACTTCTTTTTCAGCTACTCATACAATGTTATGCGTAGTCTTCAAAAGAACATGTGTGATCATAAGATAGGACATGTTGTTTATGAAACCATGTTCGTCTGGAATGAGTTCTTAACTCGGGGAATAAGGAATCACCTGCAAAATACTGTTTGGACAGTTGCATTGGTTTATGGTTTTTCTTTAGACAGGTATCTAAAACGTGGTGTTAATGAGAAGGGAAGAGTAGCTAATGATGTTGAGACCGAACAGATAGTTTTTGAGGATGTTCCTGAGGGCTTTCCTGTGCAAATAAGTTCTGTTGTACAGAACCGTGGCTCAATCCCTCTCTATTGGTCACAAGAAACATCGCTGTCAAAGAAGGATCCAAACTATGAAGCCACTAGGCTTCATTTTGAAAATCTTGCCAAGAGATATGGGAACCCCATAATTATTTTAAATCTCATCAAGACACAGGAAAGAAGGCCTCGAGAGTCCATTCTACGCACAGAGTTTGCTAATGCAATTGATTTCATTAATAAAGATCTATCTGAGGAGAACCGTCTCAGATTTCTCCACTGGGATTTGCACAAACATTCTCGAAGCAAAGCTATAAATGCTTTACTACTCTTGGGAAAGGTGGCTACATATGCCTTGACTTTAACTGGGTTCTTCTATTGTCAAGTTACACCAGAAATGGGACCCGAGGGCATGATTAATTGGCCTTCATCGGAGAATTTTGACAATTGTATGTCTCCCCAAAGACATTGTGCTAATGGAGATGAGGATGTTGATAGCATAGTGGGGAAACCAACTGGAGGCAGCAACATTGCAAATGGAAATCATTCTACCAAACCAGCCATATTCCAGAATGGGGTGCTGAGGACCAATTGCATAGACTGCCTTGATCGGACAAATGTTGCACAGTATGCATATGGGTTGGCTGCTCTAGGACACCAGCTTCATGCTTTGGGAGTTTTAGAGAATCCCAAGATAGACCTTGACGATCCATTGGCTGATGATTTAATGGGATTTTATGAAAGAATGGGTGACACACTTGCACACCAGTATGGTGGTTCAGCTGCTCACAATAAGATATTTTCTGAGAGAAGAGGTCAGTGGAGAGCAGCAACTCAGTCTCAGGAATTCTTCAGAACTCTTCAACGGTATTACAGCAATGCGTATATGGATGCACAGAAGCAAGATGCAATTAATATATTTCTTGGGTATTTTCAACCACAGCAAGATAAACCTGCACTCTGGGAACTGGATTCAGACCAGCATTACCATGATGGGAGGCATGGGTCAAAAGACATGGATGAAGATGGATGGTTCGCTTTCAAGAGATCATATTCCGATGGAAACATTCTCCGTCCAAATAGCTCACCTATGTCAGCACCAGCTGTTAAGCAGGAGAAATTTACTAACTATCCGGTTTTGCCTGATCAAACTCAACAAAGCAAGATACTTTCAGAATCCTCACCTGAAATATCAACTTGTGAGAGTGATATTGCATATTCAAGGTACACTCCCTCGATGCCTCGCAGACAACTTTTCGGAGAGTTGCAAAGATATCGGTGTCTTGAAAGTGATCATATTTACTATTCTGAACATGGAGATGGGTATAATTTCTCAAACTTTGTGGATCTAGACTGGCTATCTTCCTCTGGGAATTCATGCGAGGAGGAGCTGTATGAGAGATCGTCAATACTTACCAACTCACCATTTGAGGGGCTATCATCGGAAAATGTTGTCAACGGAATAATAGGAGATTCTACACCCTCCACAAGTGTGTATGGATCCATCTTGAAGGGAAAGGAGCAGACAGGAACAGGATTATCCTTTAGTAATGGAGAGAGTTCCAATGTTCCTGAAGACTTTCCAGATAGCTTCTCGAACTGGGTCAATTATGGAGAGACACTCTGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005811 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0009506 GO:0009653 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031982 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034596 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098805 GO:0120035 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

816

Amino Acids

92.92

Weight (kDa)

5.75

Isoelectric Point (pI)

48.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 110 - 241 2e-22 SacI homology domain
Syja_N PF02383 244 - 374 2.5e-37 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 380
AarI CACCTGC 1 cut(s) 687
Acc36I ACCTGC 2 cut(s) 687, 1780
AccB1I GGYRCC 1 cut(s) 401
AccII CGCG 1 cut(s) 419
AciI CCGC 4 cut(s) 29, 32, 274, 419
AclWI GGATC 9 cut(s) 197, 210, 442, 899, 912, 1520, 2172, 2313, 2326
AcoI YGGCCR 1 cut(s) 412
AcsI RAATTY 7 cut(s) 110, 350, 460, 1255, 1664, 1937, 2191
AcuI CTGAAG 2 cut(s) 1654, 2412
AdeI CACNNNGTG 1 cut(s) 421
AfaI GTAC 4 cut(s) 183, 279, 846, 2045
AfiI CCNNNNNNNGG 5 cut(s) 541, 1217, 1337, 1393, 2249
AflIII ACRYGT 2 cut(s) 594, 616
AleI CACNNNNGTG 1 cut(s) 2309
Alw21I GWGCWC 2 cut(s) 216, 527
Alw26I GTCTC 7 cut(s) 48, 119, 776, 1088, 1278, 1662, 2429
Alw44I GTGCAC 1 cut(s) 523
AlwI GGATC 9 cut(s) 197, 210, 442, 899, 912, 1520, 2172, 2313, 2326
Ama87I CYCGRG 3 cut(s) 660, 1008, 1223
AoxI GGCC 5 cut(s) 3, 358, 412, 1004, 1241
ApaI GGGCCC 1 cut(s) 362
ApaLI GTGCAC 1 cut(s) 523
ApeKI GCWGC 5 cut(s) 373, 1341, 1463, 1601, 1646
ApoI RAATTY 7 cut(s) 110, 350, 460, 1255, 1664, 1937, 2191
AseI ATTAAT 3 cut(s) 1056, 1236, 1731
Asp700I GAANNNNTTC 3 cut(s) 650, 1363, 2397
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 5 cut(s) 358, 359, 532, 1220, 1404
AsuHPI GGTGA 6 cut(s) 456, 668, 1580, 1896, 1992, 2232
AvaI CYCGRG 3 cut(s) 660, 1008, 1223
AvaII GGWCC 3 cut(s) 532, 1220, 1404
AxyI CCTNAGG 1 cut(s) 813
BaeGI GKGCMC 2 cut(s) 362, 527
BalI TGGCCA 1 cut(s) 414
BamHI GGATCC 3 cut(s) 202, 904, 2318
BanI GGYRCC 1 cut(s) 401
BanII GRGCYC 2 cut(s) 216, 362
BbsI GAAGAC 2 cut(s) 575, 2400
Bbv12I GWGCWC 2 cut(s) 216, 527
BbvCI CCTCAGC 2 cut(s) 82, 1400
BbvI GCAGC 5 cut(s) 360, 1353, 1450, 1588, 1658
BccI CCATC 7 cut(s) 437, 1805, 1838, 1842, 1871, 2132, 2330
BceAI ACGGC 2 cut(s) 19, 346
BcgI CGANNNNNNTGC 2 cut(s) 2075, 2109
BclI TGATCA 3 cut(s) 601, 1963, 2110
BcoDI GTCTC 7 cut(s) 48, 119, 776, 1088, 1278, 1662, 2429
BfaI CTAG 3 cut(s) 924, 1469, 2168
BfuAI ACCTGC 2 cut(s) 687, 1780
BglII AGATCT 1 cut(s) 1063
BisI GCNGC 7 cut(s) 29, 32, 374, 1342, 1464, 1602, 1647
BlsI GCNGC 7 cut(s) 30, 33, 375, 1343, 1465, 1603, 1648
Bme18I GGWCC 3 cut(s) 532, 1220, 1404
BmeT110I CYCGRG 3 cut(s) 660, 1008, 1223
BmgT120I GGNCC 5 cut(s) 358, 359, 532, 1220, 1404
BmrI ACTGGG 3 cut(s) 1108, 1194, 2428
BmsI GCATC 3 cut(s) 1699, 1714, 2046
BmuI ACTGGG 3 cut(s) 1108, 1194, 2428
BpiI GAAGAC 2 cut(s) 575, 2400
BplI GAGNNNNNCTC 1 cut(s) 2424
BpmI CTGGAG 1 cut(s) 1356
Bpu10I CCTNAGC 2 cut(s) 82, 1400
BpuEI CTTGAG 1 cut(s) 289
Bsa29I ATCGAT 1 cut(s) 196
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 2 cut(s) 853, 1224
BsaWI WCCGGW 1 cut(s) 1951
BsaXI ACNNNNNCTCC 2 cut(s) 2195, 2225
Bsc4I CCNNNNNNNGG 5 cut(s) 541, 1217, 1337, 1393, 2249
Bse1I ACTGG 8 cut(s) 304, 1103, 1189, 1339, 1585, 1791, 2177, 2423
Bse21I CCTNAGG 1 cut(s) 813
Bse3DI GCAATG 2 cut(s) 1347, 1702
BseCI ATCGAT 1 cut(s) 196
BseDI CCNNGG 2 cut(s) 853, 1224
BseGI GGATG 5 cut(s) 811, 1312, 1714, 1843, 1853
BseLI CCNNNNNNNGG 5 cut(s) 541, 1217, 1337, 1393, 2249
BseMI GCAATG 2 cut(s) 1347, 1702
BseMII CTCAG 8 cut(s) 96, 804, 1062, 1099, 1391, 1614, 1667, 1673
BseNI ACTGG 8 cut(s) 304, 1103, 1189, 1339, 1585, 1791, 2177, 2423
BseRI GAGGAG 5 cut(s) 66, 66, 1088, 2216, 2219
BseSI GKGCMC 2 cut(s) 362, 527
BseXI GCAGC 5 cut(s) 360, 1353, 1450, 1588, 1658
BsgI GTGCAG 2 cut(s) 496, 1758
Bsh1236I CGCG 1 cut(s) 419
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 5 cut(s) 5, 360, 414, 1006, 1243
BshNI GGYRCC 1 cut(s) 401
BshVI ATCGAT 1 cut(s) 196
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 2 cut(s) 216, 527
BsiHKCI CYCGRG 3 cut(s) 660, 1008, 1223
BsiSI CCGG 3 cut(s) 23, 48, 1952
BslFI GGGAC 2 cut(s) 170, 1233
BslI CCNNNNNNNGG 5 cut(s) 541, 1217, 1337, 1393, 2249
BsmAI GTCTC 7 cut(s) 48, 119, 776, 1088, 1278, 1662, 2429
BsmBI CGTCTC 2 cut(s) 48, 1088
BsmFI GGGAC 2 cut(s) 170, 1233
BsnI GGCC 5 cut(s) 5, 360, 414, 1006, 1243
Bso31I GGTCTC 1 cut(s) 776
BsoBI CYCGRG 3 cut(s) 660, 1008, 1223
Bsp120I GGGCCC 1 cut(s) 358
Bsp1286I GDGCHC 3 cut(s) 216, 362, 527
Bsp1407I TGTACA 1 cut(s) 844
BspACI CCGC 4 cut(s) 29, 32, 274, 419
BspANI GGCC 5 cut(s) 5, 360, 414, 1006, 1243
BspCNI CTCAG 8 cut(s) 95, 805, 1063, 1098, 1392, 1615, 1666, 1672
BspDI ATCGAT 1 cut(s) 196
BspFNI CGCG 1 cut(s) 419
BspMI ACCTGC 2 cut(s) 687, 1780
BspPI GGATC 9 cut(s) 197, 210, 442, 899, 912, 1520, 2172, 2313, 2326
BspT107I GGYRCC 1 cut(s) 401
BspTNI GGTCTC 1 cut(s) 776
BsrDI GCAATG 2 cut(s) 1347, 1702
BsrGI TGTACA 1 cut(s) 844
BsrI ACTGG 8 cut(s) 304, 1103, 1189, 1339, 1585, 1791, 2177, 2423
BssECI CCNNGG 2 cut(s) 853, 1224
Bst4CI ACNGT 6 cut(s) 691, 700, 854, 1082, 1446, 1686
Bst6I CTCTTC 3 cut(s) 757, 1624, 1683
BstAPI GCANNNNNTGC 2 cut(s) 1419, 1448
BstAUI TGTACA 1 cut(s) 844
BstDEI CTNAG 8 cut(s) 82, 813, 1071, 1085, 1400, 1623, 1653, 1659
BstDSI CCRYGG 1 cut(s) 853
BstENI CCTNNNNNAGG 1 cut(s) 539
BstF5I GGATG 5 cut(s) 811, 1312, 1714, 1843, 1853
BstFNI CGCG 1 cut(s) 419
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 7 cut(s) 48, 119, 776, 1088, 1278, 1662, 2429
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 6 cut(s) 28, 425, 1136, 1169, 1419, 1448
BstNSI RCATGY 3 cut(s) 373, 598, 620
BstSLI GKGCMC 2 cut(s) 362, 527
BstUI CGCG 1 cut(s) 419
BstV1I GCAGC 5 cut(s) 360, 1353, 1450, 1588, 1658
BstV2I GAAGAC 2 cut(s) 575, 2400
BstX2I RGATCY 5 cut(s) 202, 904, 1063, 2164, 2318
BstXI CCANNNNNNTGG 1 cut(s) 1592
BstYI RGATCY 5 cut(s) 202, 904, 1063, 2164, 2318
Bsu15I ATCGAT 1 cut(s) 196
Bsu36I CCTNAGG 1 cut(s) 813
BsuRI GGCC 5 cut(s) 5, 360, 414, 1006, 1243
BsuTUI ATCGAT 1 cut(s) 196
BtgI CCRYGG 1 cut(s) 853
BtgZI GCGATG 1 cut(s) 873
BtsCI GGATG 5 cut(s) 811, 1312, 1714, 1843, 1853
BtsIMutI CAGTG 5 cut(s) 297, 479, 1096, 1643, 2446
BveI ACCTGC 2 cut(s) 687, 1780
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 5 cut(s) 358, 359, 532, 1220, 1404
ClaI ATCGAT 1 cut(s) 196
Csp6I GTAC 4 cut(s) 182, 278, 845, 2044
CspCI CAANNNNNGTGG 2 cut(s) 2142, 2177
CviQI GTAC 4 cut(s) 182, 278, 845, 2044
DdeI CTNAG 8 cut(s) 82, 813, 1071, 1085, 1400, 1623, 1653, 1659
DraI TTTAAA 1 cut(s) 978
DraIII CACNNNGTG 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 412
Eam1104I CTCTTC 3 cut(s) 757, 1624, 1683
EarI CTCTTC 3 cut(s) 757, 1624, 1683
Ecl136II GAGCTC 1 cut(s) 214
Eco147I AGGCCT 1 cut(s) 1006
Eco24I GRGCYC 2 cut(s) 216, 362
Eco31I GGTCTC 1 cut(s) 776
Eco32I GATATC 1 cut(s) 2093
Eco47I GGWCC 3 cut(s) 532, 1220, 1404
Eco53kI GAGCTC 1 cut(s) 214
Eco57I CTGAAG 2 cut(s) 1654, 2412
Eco81I CCTNAGG 1 cut(s) 813
Eco88I CYCGRG 3 cut(s) 660, 1008, 1223
EcoICRI GAGCTC 1 cut(s) 214
EcoNI CCTNNNNNAGG 1 cut(s) 539
EcoO109I RGGNCCY 2 cut(s) 359, 1220
EcoRI GAATTC 2 cut(s) 1664, 2191
EcoRV GATATC 1 cut(s) 2093
EcoT22I ATGCAT 1 cut(s) 1453
EcoT38I GRGCYC 2 cut(s) 216, 362
Esp3I CGTCTC 2 cut(s) 48, 1088
FalI AAGNNNNNCTT 4 cut(s) 503, 535, 533, 565
FaqI GGGAC 2 cut(s) 170, 1233
FauI CCCGC 1 cut(s) 267
FauNDI CATATG 2 cut(s) 1168, 1453
FbaI TGATCA 3 cut(s) 601, 1963, 2110
Fnu4HI GCNGC 7 cut(s) 29, 32, 374, 1342, 1464, 1602, 1647
FokI GGATG 5 cut(s) 818, 1319, 1721, 1850, 1860
FriOI GRGCYC 2 cut(s) 216, 362
Fsp4HI GCNGC 7 cut(s) 29, 32, 374, 1342, 1464, 1602, 1647
FspBI CTAG 3 cut(s) 924, 1469, 2168
GlaI GCGC 1 cut(s) 21
GluI GCNGC 7 cut(s) 29, 32, 374, 1342, 1464, 1602, 1647
GsuI CTGGAG 1 cut(s) 1356
HaeIII GGCC 5 cut(s) 5, 360, 414, 1006, 1243
HapII CCGG 3 cut(s) 23, 48, 1952
HhaI GCGC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 1 cut(s) 2275
HindII GTYRAC 1 cut(s) 2275
HinfI GANTC 8 cut(s) 127, 230, 673, 1013, 1504, 1790, 1994, 2291
HpaII CCGG 3 cut(s) 23, 48, 1952
HphI GGTGA 6 cut(s) 456, 668, 1580, 1896, 1992, 2232
Hpy166II GTNNAC 3 cut(s) 525, 2046, 2275
Hpy8I GTNNAC 3 cut(s) 525, 2046, 2275
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 7 cut(s) 147, 278, 751, 895, 996, 1254, 2323
HpyCH4III ACNGT 6 cut(s) 691, 700, 854, 1082, 1446, 1686
HpyCH4IV ACGT 3 cut(s) 170, 250, 742
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 425, 1136, 1169, 1419, 1448
HpyF3I CTNAG 8 cut(s) 82, 813, 1071, 1085, 1400, 1623, 1653, 1659
HpySE526I ACGT 3 cut(s) 170, 250, 742
HspAI GCGC 1 cut(s) 20
KflI GGGWCCC 1 cut(s) 1220
Ksp22I TGATCA 3 cut(s) 601, 1963, 2110
LmnI GCTCC 3 cut(s) 25, 2206, 2338
Lsp1109I GCAGC 5 cut(s) 360, 1353, 1450, 1588, 1658
LweI GCATC 3 cut(s) 1699, 1714, 2046
MaeI CTAG 3 cut(s) 924, 1469, 2168
MaeII ACGT 3 cut(s) 170, 250, 742
MaeIII GTNAC 6 cut(s) 295, 316, 326, 876, 1204, 1568
MfeI CAATTG 3 cut(s) 1044, 1264, 1408
MflI RGATCY 5 cut(s) 202, 904, 1063, 2164, 2318
MhlI GDGCHC 3 cut(s) 216, 362, 527
MlsI TGGCCA 1 cut(s) 414
MluNI TGGCCA 1 cut(s) 414
MlyI GAGTC 2 cut(s) 121, 1022
Mox20I TGGCCA 1 cut(s) 414
Mph1103I ATGCAT 1 cut(s) 1453
MroXI GAANNNNTTC 3 cut(s) 650, 1363, 2397
MscI TGGCCA 1 cut(s) 414
MslI CAYNNNNRTG 5 cut(s) 101, 1587, 1806, 2136, 2309
Msp20I TGGCCA 1 cut(s) 414
MspA1I CMGCKG 2 cut(s) 1601, 1922
MspI CCGG 3 cut(s) 23, 48, 1952
MunI CAATTG 3 cut(s) 1044, 1264, 1408
MvnI CGCG 1 cut(s) 419
MwoI GCNNNNNNNGC 6 cut(s) 28, 425, 1136, 1169, 1419, 1448
NdeI CATATG 2 cut(s) 1168, 1453
NmuCI GTSAC 4 cut(s) 295, 316, 876, 1568
NsiI ATGCAT 1 cut(s) 1453
NspI RCATGY 3 cut(s) 373, 598, 620
OliI CACNNNNGTG 1 cut(s) 2309
PaeR7I CTCGAG 1 cut(s) 1008
PaqCI CACCTGC 1 cut(s) 687
PceI AGGCCT 1 cut(s) 1006
PciI ACATGT 2 cut(s) 594, 616
PdmI GAANNNNTTC 3 cut(s) 650, 1363, 2397
PfeI GAWTC 6 cut(s) 230, 673, 1504, 1790, 1994, 2291
PkrI GCNGC 7 cut(s) 30, 33, 375, 1343, 1465, 1603, 1648
Ple19I CGATCG 1 cut(s) 134
PleI GAGTC 2 cut(s) 121, 1021
PpsI GAGTC 2 cut(s) 121, 1021
PpuMI RGGWCCY 1 cut(s) 1220
PscI ACATGT 2 cut(s) 594, 616
PshBI ATTAAT 3 cut(s) 1056, 1236, 1731
PsiI TTATAA 1 cut(s) 380
Psp124BI GAGCTC 1 cut(s) 216
Psp5II RGGWCCY 1 cut(s) 1220
PspOMI GGGCCC 1 cut(s) 358
PspPI GGNCC 5 cut(s) 358, 359, 532, 1220, 1404
PspPPI RGGWCCY 1 cut(s) 1220
PsuI RGATCY 5 cut(s) 202, 904, 1063, 2164, 2318
PvuI CGATCG 1 cut(s) 134
PvuII CAGCTG 2 cut(s) 1601, 1922
RsaI GTAC 4 cut(s) 183, 279, 846, 2045
RsaNI GTAC 4 cut(s) 182, 278, 845, 2044
RseI CAYNNNNRTG 5 cut(s) 101, 1587, 1806, 2136, 2309
SacI GAGCTC 1 cut(s) 216
SatI GCNGC 7 cut(s) 29, 32, 374, 1342, 1464, 1602, 1647
Sau96I GGNCC 5 cut(s) 358, 359, 532, 1220, 1404
SchI GAGTC 2 cut(s) 121, 1022
SduI GDGCHC 3 cut(s) 216, 362, 527
SfaNI GCATC 3 cut(s) 1699, 1714, 2046
Sfr274I CTCGAG 1 cut(s) 1008
SinI GGWCC 3 cut(s) 532, 1220, 1404
SlaI CTCGAG 1 cut(s) 1008
SmiMI CAYNNNNRTG 5 cut(s) 101, 1587, 1806, 2136, 2309
SmlI CTYRAG 2 cut(s) 268, 1008
SmoI CTYRAG 2 cut(s) 268, 1008
SseBI AGGCCT 1 cut(s) 1006
SsiI CCGC 4 cut(s) 29, 32, 274, 419
SspMI CTAG 3 cut(s) 924, 1469, 2168
SstI GAGCTC 1 cut(s) 216
StuI AGGCCT 1 cut(s) 1006
TaaI ACNGT 6 cut(s) 691, 700, 854, 1082, 1446, 1686
TaiI ACGT 3 cut(s) 173, 253, 745
TaqI TCGA 7 cut(s) 130, 134, 196, 1009, 1120, 2054, 2414
TaqII GACCGA 1 cut(s) 800
TatI WGTACW 1 cut(s) 844
TauI GCSGC 2 cut(s) 31, 34
TfiI GAWTC 6 cut(s) 230, 673, 1504, 1790, 1994, 2291
TscAI CASTG 4 cut(s) 304, 479, 1103, 1643
TseFI GTSAC 4 cut(s) 295, 316, 876, 1568
TseI GCWGC 5 cut(s) 373, 1341, 1463, 1601, 1646
Tsp45I GTSAC 4 cut(s) 295, 316, 876, 1568
TspGWI ACGGA 3 cut(s) 260, 1880, 2292
TspRI CASTG 4 cut(s) 304, 479, 1103, 1643
VneI GTGCAC 1 cut(s) 523
VpaK11BI GGWCC 3 cut(s) 532, 1220, 1404
VspI ATTAAT 3 cut(s) 1056, 1236, 1731
XagI CCTNNNNNAGG 1 cut(s) 539
XapI RAATTY 7 cut(s) 110, 350, 460, 1255, 1664, 1937, 2191
XbaI TCTAGA 1 cut(s) 2167
XceI RCATGY 3 cut(s) 373, 598, 620
XhoI CTCGAG 1 cut(s) 1008
XmnI GAANNNNTTC 3 cut(s) 650, 1363, 2397
XspI CTAG 3 cut(s) 924, 1469, 2168
Zsp2I ATGCAT 1 cut(s) 1453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.