Rorug02G0054100

component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is involved in protein synthesis of a specialized repertoire of mRNAs and, together with other initiation factors, stimulates binding of mRNA and methionyl-tRNAi to the 40S ribosome. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0007042
Physical Location & Seq
Forward (+)
4146993 .. 4150534
3542 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0054100.1

Sequence Viewer

Length: 1248 bp
ATGCGAAGCTTTTTCGGAGCGACCCAAATTCTGAGCAAGTCTTCATCTTTCTTCCTACCCACAAATCCCAACCTCTCCTTCTCCAAAACCCCCATCTTCCTCCGAACCTTCGCGATCATGGCCACCGCCACCGACGAGCTCGTCAAGGGCGCCGTTCACCCAAACGGCGTCGCTGTGATCACCCTCGATCGCCCCAAAGCTCTCAACGCCATGAACATAGATATGGATTTGAAATTCAAGAGCTATCTGGATCAGTGGGAATCGGACCCGAGTGTGAAGTGTGTTTTGGTCGATAGCAGCTCAGCTCGTGCCTTTTGTGCTGGCGGTGATGTGAAGCAGATCACGGCCAAAAACCAACTATCCGATGTGATTGAGGTATTCACTGCTGAGTATTCTTTAATATGCAAAATATCTAACTACAAGAAGCCGTATGTAAGTTTCATGGATGGCATAACAATGGGCTTTGGAATTGGCCTATCAGGTCATGGTCGCTACCGGATCATTACAGAGAGGACTCTTCTTGCTATGCCAGAAAATGGTATTGGATTGTTTCCAGACGTTGGCTTTTCACATATAGCGGCACAGAGTCCAGGAGGAGGATCAGTTGGTGCTTATCTTGGAATCACTGGGAAAAGAATTTCGACGCCGTCGGATGCACTCTATGTAGGTCTTGGGACACATTTTGTGCCATCTCAAAACTTAGGTTCAGTAAAGGAGACCCTTTTGTCAACTAACTTTTCCCAGGATCCTCATGAGGACATCAAAACGATTTTGGCGAAGTTCAGCAGTAAACCTGATTCCGAATCCCAACTGAAGCAGCTTTTACCTCAAATAGCCTCATCATTTGGTGCAAATAAGTCAGTTATTGGGACAATTGAAGAACTCAAAAAGCATCAATCGAGTTCAAATGCTACTGTGGTGGAGTGGGCTAATGAAGCTCTTCAAGGTCTTGGAAAGGGTGCTCCTTTTTCACTTTTCTTGACACAGAAGTATTTCTCCAAAGTTGCATCTGCCCTTGGGAAGAATGACAATCAATTATCCACACTGACTGGTGTCATGAAAACTGAATATCGCATTGCTGTAAGATCATCCTTGCGTAATGACTTTGCTGAAGGTGTGCGGGCAGTGTTGGTGGACAAGGATCAGAATCCAAAGTGGAACCCGTCAAGGTTGGAGGAAGTTAATGAGAACGAAGTGGAAGCTCTGTTTGAACCATTGAGCCCAAATGTGGAGGAGTTGAGCGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005852 GO:0032991 GO:0044424 GO:0044444 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CSN8_PSD8_EIF3K

Protein Analysis

415

Amino Acids

45.06

Weight (kDa)

6.62

Isoelectric Point (pI)

34.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ECH_1 PF00378 54 - 262 1.6e-24 Enoyl-CoA hydratase/isomerase
ECH_2 PF16113 58 - 403 8.4e-106 Enoyl-CoA hydratase/isomerase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 140, 724
AccB1I GGYRCC 1 cut(s) 149
AccB7I CCANNNNNTGG 2 cut(s) 536, 560
AccII CGCG 1 cut(s) 113
AciI CCGC 4 cut(s) 126, 324, 578, 1120
AclWI GGATC 6 cut(s) 258, 506, 607, 740, 753, 1149
AcoI YGGCCR 2 cut(s) 120, 345
AcsI RAATTY 3 cut(s) 27, 233, 636
AcuI CTGAAG 2 cut(s) 833, 1131
AcyI GRCGYC 3 cut(s) 150, 168, 644
AfiI CCNNNNNNNGG 4 cut(s) 536, 560, 596, 1228
AgsI TTSAA 6 cut(s) 232, 238, 878, 906, 944, 1211
AjnI CCWGG 2 cut(s) 589, 741
AjuI GAANNNNNNNTTGG 6 cut(s) 62, 94, 269, 301, 525, 557
AluBI AGCT 9 cut(s) 9, 139, 200, 243, 300, 305, 820, 938, 1202
AluI AGCT 9 cut(s) 9, 139, 200, 243, 300, 305, 820, 938, 1202
Alw21I GWGCWC 2 cut(s) 141, 964
Alw26I GTCTC 1 cut(s) 710
AlwI GGATC 6 cut(s) 258, 506, 607, 740, 753, 1149
Ama87I CYCGRG 1 cut(s) 268
AoxI GGCC 3 cut(s) 120, 345, 472
ApeKI GCWGC 2 cut(s) 297, 817
ApoI RAATTY 3 cut(s) 27, 233, 636
Asp700I GAANNNNTTC 2 cut(s) 939, 992
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 3 cut(s) 149, 172, 338
AvaI CYCGRG 1 cut(s) 268
AvaII GGWCC 1 cut(s) 265
BalI TGGCCA 1 cut(s) 122
BamHI GGATCC 1 cut(s) 745
BanI GGYRCC 1 cut(s) 149
BanII GRGCYC 2 cut(s) 141, 1223
BauI CACGAG 1 cut(s) 306
BbsI GAAGAC 1 cut(s) 33
Bbv12I GWGCWC 2 cut(s) 141, 964
BbvI GCAGC 2 cut(s) 309, 829
BccI CCATC 3 cut(s) 101, 440, 697
BceAI ACGGC 5 cut(s) 137, 181, 360, 412, 631
BciT130I CCWGG 2 cut(s) 591, 743
BclI TGATCA 1 cut(s) 177
BcoDI GTCTC 1 cut(s) 710
BfoI RGCGCY 1 cut(s) 153
BisI GCNGC 3 cut(s) 298, 579, 818
BlpI GCTNAGC 1 cut(s) 301
BlsI GCNGC 3 cut(s) 299, 580, 819
Bme1390I CCNGG 2 cut(s) 591, 743
Bme18I GGWCC 1 cut(s) 265
BmeT110I CYCGRG 1 cut(s) 268
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 4 cut(s) 151, 267, 747, 1160
BmrFI CCNGG 2 cut(s) 591, 743
BmrI ACTGGG 1 cut(s) 636
BmsI GCATC 3 cut(s) 643, 901, 1016
BmuI ACTGGG 1 cut(s) 636
BoxI GACNNNNGTC 1 cut(s) 1052
BpiI GAAGAC 1 cut(s) 33
Bpu1102I GCTNAGC 1 cut(s) 301
BsaBI GATNNNNATC 1 cut(s) 1146
BsaHI GRCGYC 3 cut(s) 150, 168, 644
BsaI GGTCTC 1 cut(s) 710
BsaJI CCNNGG 2 cut(s) 741, 1015
BsaWI WCCGGW 1 cut(s) 495
BsaXI ACNNNNNCTCC 2 cut(s) 588, 618
Bsc4I CCNNNNNNNGG 4 cut(s) 536, 560, 596, 1228
Bse1I ACTGG 2 cut(s) 631, 1054
Bse3DI GCAATG 1 cut(s) 1074
Bse8I GATNNNNATC 1 cut(s) 1146
BseBI CCWGG 2 cut(s) 591, 743
BseDI CCNNGG 2 cut(s) 741, 1015
BseGI GGATG 3 cut(s) 451, 658, 1088
BseJI GATNNNNATC 1 cut(s) 1146
BseLI CCNNNNNNNGG 4 cut(s) 536, 560, 596, 1228
BseMI GCAATG 1 cut(s) 1074
BseMII CTCAG 3 cut(s) 23, 315, 378
BseNI ACTGG 2 cut(s) 631, 1054
BseRI GAGGAG 2 cut(s) 609, 1247
BseXI GCAGC 2 cut(s) 309, 829
Bsh1236I CGCG 1 cut(s) 113
Bsh1285I CGRYCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 122, 347, 474
BshNI GGYRCC 1 cut(s) 149
BsiEI CGRYCG 1 cut(s) 190
BsiHKAI GWGCWC 2 cut(s) 141, 964
BsiHKCI CYCGRG 1 cut(s) 268
BsiSI CCGG 1 cut(s) 496
BslFI GGGAC 2 cut(s) 688, 883
BslI CCNNNNNNNGG 4 cut(s) 536, 560, 596, 1228
BsmAI GTCTC 1 cut(s) 710
BsmFI GGGAC 2 cut(s) 688, 883
BsnI GGCC 3 cut(s) 122, 347, 474
Bso31I GGTCTC 1 cut(s) 710
BsoBI CYCGRG 1 cut(s) 268
Bsp1286I GDGCHC 3 cut(s) 141, 964, 1223
Bsp1720I GCTNAGC 1 cut(s) 301
Bsp68I TCGCGA 1 cut(s) 113
BspACI CCGC 4 cut(s) 126, 324, 578, 1120
BspANI GGCC 3 cut(s) 122, 347, 474
BspCNI CTCAG 3 cut(s) 24, 314, 379
BspFNI CGCG 1 cut(s) 113
BspHI TCATGA 2 cut(s) 751, 1056
BspLI GGNNCC 4 cut(s) 151, 267, 747, 1160
BspPI GGATC 6 cut(s) 258, 506, 607, 740, 753, 1149
BspQI GCTCTTC 1 cut(s) 945
BspT107I GGYRCC 1 cut(s) 149
BspTNI GGTCTC 1 cut(s) 710
BsrDI GCAATG 1 cut(s) 1074
BsrI ACTGG 2 cut(s) 631, 1054
BssECI CCNNGG 2 cut(s) 741, 1015
BssNI GRCGYC 3 cut(s) 150, 168, 644
BssSI CACGAG 1 cut(s) 306
BssT1I CCWWGG 1 cut(s) 1015
Bst2BI CACGAG 1 cut(s) 306
Bst2UI CCWGG 2 cut(s) 591, 743
Bst4CI ACNGT 1 cut(s) 916
Bst6I CTCTTC 2 cut(s) 522, 945
BstACI GRCGYC 3 cut(s) 150, 168, 644
BstC8I GCNNGC 2 cut(s) 322, 1122
BstDEI CTNAG 4 cut(s) 32, 301, 387, 700
BstF5I GGATG 3 cut(s) 451, 658, 1088
BstFNI CGCG 1 cut(s) 113
BstH2I RGCGCY 1 cut(s) 153
BstHHI GCGC 1 cut(s) 152
BstMAI GTCTC 1 cut(s) 710
BstMCI CGRYCG 1 cut(s) 190
BstMWI GCNNNNNNNGC 4 cut(s) 119, 206, 317, 935
BstNI CCWGG 2 cut(s) 591, 743
BstPAI GACNNNNGTC 1 cut(s) 1052
BstSCI CCNGG 2 cut(s) 589, 741
BstUI CGCG 1 cut(s) 113
BstV1I GCAGC 2 cut(s) 309, 829
BstV2I GAAGAC 1 cut(s) 33
BstX2I RGATCY 1 cut(s) 745
BstYI RGATCY 1 cut(s) 745
BsuRI GGCC 3 cut(s) 122, 347, 474
BtsCI GGATG 3 cut(s) 451, 658, 1088
BtsI GCAGTG 2 cut(s) 381, 1131
BtsIMutI CAGTG 5 cut(s) 260, 381, 624, 1043, 1131
BtuMI TCGCGA 1 cut(s) 113
Cac8I GCNNGC 2 cut(s) 322, 1122
CciI TCATGA 2 cut(s) 751, 1056
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 1 cut(s) 265
CseI GACGC 2 cut(s) 157, 652
CviAII CATG 6 cut(s) 118, 211, 442, 485, 752, 1057
DdeI CTNAG 4 cut(s) 32, 301, 387, 700
DinI GGCGCC 1 cut(s) 151
DrdI GACNNNNNNGTC 2 cut(s) 140, 724
DseDI GACNNNNNNGTC 2 cut(s) 140, 724
EaeI YGGCCR 2 cut(s) 120, 345
Eam1104I CTCTTC 2 cut(s) 522, 945
EarI CTCTTC 2 cut(s) 522, 945
Ecl136II GAGCTC 1 cut(s) 139
Eco130I CCWWGG 1 cut(s) 1015
Eco24I GRGCYC 2 cut(s) 141, 1223
Eco31I GGTCTC 1 cut(s) 710
Eco47I GGWCC 1 cut(s) 265
Eco53kI GAGCTC 1 cut(s) 139
Eco57I CTGAAG 2 cut(s) 833, 1131
Eco88I CYCGRG 1 cut(s) 268
EcoICRI GAGCTC 1 cut(s) 139
EcoRII CCWGG 2 cut(s) 589, 741
EcoT14I CCWWGG 1 cut(s) 1015
EcoT38I GRGCYC 2 cut(s) 141, 1223
EgeI GGCGCC 1 cut(s) 151
EheI GGCGCC 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 1015
FaeI CATG 6 cut(s) 121, 214, 445, 488, 755, 1060
FaqI GGGAC 2 cut(s) 688, 883
FatI CATG 6 cut(s) 117, 210, 441, 484, 751, 1056
FauI CCCGC 1 cut(s) 1113
FbaI TGATCA 1 cut(s) 177
Fnu4HI GCNGC 3 cut(s) 298, 579, 818
FokI GGATG 3 cut(s) 458, 665, 1075
FriOI GRGCYC 2 cut(s) 141, 1223
Fsp4HI GCNGC 3 cut(s) 298, 579, 818
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 298, 579, 818
HaeII RGCGCY 1 cut(s) 153
HaeIII GGCC 3 cut(s) 122, 347, 474
HapII CCGG 1 cut(s) 496
HgaI GACGC 2 cut(s) 157, 652
HhaI GCGC 1 cut(s) 152
Hin1I GRCGYC 3 cut(s) 150, 168, 644
Hin1II CATG 6 cut(s) 121, 214, 445, 488, 755, 1060
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 1 cut(s) 729
HindII GTYRAC 1 cut(s) 729
HindIII AAGCTT 1 cut(s) 7
HinfI GANTC 7 cut(s) 260, 514, 586, 621, 797, 803, 1147
HpaII CCGG 1 cut(s) 496
HphI GGTGA 3 cut(s) 149, 172, 338
Hpy166II GTNNAC 4 cut(s) 157, 729, 791, 1135
Hpy188I TCNGA 8 cut(s) 17, 33, 104, 265, 364, 652, 802, 1146
Hpy188III TCNNGA 7 cut(s) 112, 238, 248, 554, 752, 979, 1057
Hpy8I GTNNAC 4 cut(s) 157, 729, 791, 1135
Hpy99I CGWCG 4 cut(s) 137, 173, 646, 652
HpyAV CCTTC 3 cut(s) 88, 118, 1106
HpyCH4III ACNGT 1 cut(s) 916
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 4 cut(s) 405, 656, 851, 1007
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 206, 317, 935
HpyF3I CTNAG 4 cut(s) 32, 301, 387, 700
HpySE526I ACGT 1 cut(s) 558
Hsp92I GRCGYC 3 cut(s) 150, 168, 644
Hsp92II CATG 6 cut(s) 121, 214, 445, 488, 755, 1060
HspAI GCGC 1 cut(s) 150
KasI GGCGCC 1 cut(s) 149
Ksp22I TGATCA 1 cut(s) 177
LguI GCTCTTC 1 cut(s) 945
LmnI GCTCC 2 cut(s) 17, 967
Lsp1109I GCAGC 2 cut(s) 309, 829
LweI GCATC 3 cut(s) 643, 901, 1016
MaeII ACGT 1 cut(s) 558
MboII GAAGA 7 cut(s) 33, 43, 88, 509, 890, 932, 1033
MfeI CAATTG 1 cut(s) 873
MflI RGATCY 1 cut(s) 745
MhlI GDGCHC 3 cut(s) 141, 964, 1223
MlsI TGGCCA 1 cut(s) 122
MluCI AATT 6 cut(s) 27, 233, 468, 636, 873, 1034
MluNI TGGCCA 1 cut(s) 122
Mly113I GGCGCC 1 cut(s) 150
MlyI GAGTC 2 cut(s) 508, 595
MmeI TCCRAC 2 cut(s) 630, 1152
Mox20I TGGCCA 1 cut(s) 122
MroXI GAANNNNTTC 2 cut(s) 939, 992
MscI TGGCCA 1 cut(s) 122
MseI TTAA 2 cut(s) 398, 1182
MslI CAYNNNNRTG 2 cut(s) 221, 455
Msp20I TGGCCA 1 cut(s) 122
MspI CCGG 1 cut(s) 496
MspR9I CCNGG 2 cut(s) 591, 743
MunI CAATTG 1 cut(s) 873
MvaI CCWGG 2 cut(s) 591, 743
MvnI CGCG 1 cut(s) 113
MwoI GCNNNNNNNGC 4 cut(s) 119, 206, 317, 935
NarI GGCGCC 1 cut(s) 150
NlaIII CATG 6 cut(s) 121, 214, 445, 488, 755, 1060
NlaIV GGNNCC 4 cut(s) 151, 267, 747, 1160
NruI TCGCGA 1 cut(s) 113
PagI TCATGA 2 cut(s) 751, 1056
PciSI GCTCTTC 1 cut(s) 945
PcsI WCGNNNNNNNCGW 1 cut(s) 773
PdmI GAANNNNTTC 2 cut(s) 939, 992
PfeI GAWTC 5 cut(s) 260, 621, 797, 803, 1147
PflFI GACNNNGTC 1 cut(s) 646
PflMI CCANNNNNTGG 2 cut(s) 536, 560
PfoI TCCNGGA 1 cut(s) 589
PkrI GCNGC 3 cut(s) 299, 580, 819
Ple19I CGATCG 1 cut(s) 190
PleI GAGTC 2 cut(s) 508, 594
PluTI GGCGCC 1 cut(s) 153
PpsI GAGTC 2 cut(s) 508, 594
PshAI GACNNNNGTC 1 cut(s) 1052
Psp124BI GAGCTC 1 cut(s) 141
Psp6I CCWGG 2 cut(s) 589, 741
PspGI CCWGG 2 cut(s) 589, 741
PspN4I GGNNCC 4 cut(s) 151, 267, 747, 1160
PspPI GGNCC 1 cut(s) 265
PsuI RGATCY 1 cut(s) 745
PsyI GACNNNGTC 1 cut(s) 646
PvuI CGATCG 1 cut(s) 190
RruI TCGCGA 1 cut(s) 113
RseI CAYNNNNRTG 2 cut(s) 221, 455
SacI GAGCTC 1 cut(s) 141
SapI GCTCTTC 1 cut(s) 945
SaqAI TTAA 2 cut(s) 398, 1182
SatI GCNGC 3 cut(s) 298, 579, 818
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 2 cut(s) 508, 595
ScrFI CCNGG 2 cut(s) 591, 743
SduI GDGCHC 3 cut(s) 141, 964, 1223
SfaNI GCATC 3 cut(s) 643, 901, 1016
SfoI GGCGCC 1 cut(s) 151
SinI GGWCC 1 cut(s) 265
SmiMI CAYNNNNRTG 2 cut(s) 221, 455
Sse9I AATT 6 cut(s) 27, 233, 468, 636, 873, 1034
SsiI CCGC 4 cut(s) 126, 324, 578, 1120
SspDI GGCGCC 1 cut(s) 149
SstI GAGCTC 1 cut(s) 141
StyD4I CCNGG 2 cut(s) 589, 741
StyI CCWWGG 1 cut(s) 1015
TaaI ACNGT 1 cut(s) 916
TaiI ACGT 1 cut(s) 561
TaqI TCGA 4 cut(s) 186, 291, 641, 899
TasI AATT 6 cut(s) 27, 233, 468, 636, 873, 1034
TauI GCSGC 1 cut(s) 581
TfiI GAWTC 5 cut(s) 260, 621, 797, 803, 1147
Tru1I TTAA 2 cut(s) 398, 1182
Tru9I TTAA 2 cut(s) 398, 1182
TscAI CASTG 5 cut(s) 260, 388, 631, 1050, 1131
TseI GCWGC 2 cut(s) 297, 817
TspDTI ATGAA 5 cut(s) 33, 227, 430, 948, 1073
TspRI CASTG 5 cut(s) 260, 388, 631, 1050, 1131
Tth111I GACNNNGTC 1 cut(s) 646
Van91I CCANNNNNTGG 2 cut(s) 536, 560
VpaK11BI GGWCC 1 cut(s) 265
XapI RAATTY 3 cut(s) 27, 233, 636
XmnI GAANNNNTTC 2 cut(s) 939, 992
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.