Rh2AG103300

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0007042
Physical Location & Seq
Reverse (-)
8865967 .. 8874445
8479 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG103300.1

Sequence Viewer

Length: 2475 bp
ATGGCCTCGTCGGAAAATGCGCCGGAGCAGCCGCCGTCCTCCCACCCCGGAGGAGACGAGGAAGTTATTACTCCTCCTCAGCACATAGATATGCAGAAATTCAGGCTTTACGAGACTCGATCGAACTTTTACATGATTGGAAGAGACAAGAGTAGAACGTATTGGAGGGTACTAAAGATTGATCGATTGGATCCCTGTGAGCTCAGTGTTCTTGAGGATTCTACTACTTATACCGAACGTGAATGTTCTGACCTCTTGAGGCGGGTACATGAAGGGAACAAGGTCACTGGCGGATTGAAGTTTGTCACTACTTGTTACGGCATTATTGGGTTCATTAAATTTCTGGGGCCCTATTACATGCTGCTTATAACAAAGAGAAGAAAGATAGGTGCCATTCGTGGCCATGCGGTGTATGCCATCTCCAAAAGTGAAATGATCCCACTTCCAAATTCACCTGTTCAGTGCAGTATCGCTGATTCTAGAGATGAAGACAGATACAAGAAGCTCCTGTGCACAATGGACCTCACAAAGGACTTCTTTTTCAGCTACTCATACAATGTTATGCGTAGTCTTCAAAAGAACATGTGTGATCATAAGATGGGACATGTTGTTTATGAAACCATGTTCGTCTGGAATGAGTTCTTAACTCGGGGAATAAGGAATCACCTGCAAAATACTGTTTGGACAGTTGCATTGGTTTATGGTTTCTTTAGACAGGCCACTCTCTCTACATCTGGACGTGACTTCAAACTGACCCTCATTGCAAGACGTTCACGTCACTATGCGGGAACCAGGTATCTAAAACGTGGTGTTAATGAGAAGGGAAGAGTAGCTAATGATGTTGAGACCGAACAGATAGTTTTTGAGGATGTTCCTGAGGGCTTTCCTGTGCAAATAAGTTCTGTTGTACAGAACCGTGGCTCAATCCCTCTCTATTGGTCACAAGAAACATCGCGTTTAAACATTAAACCAGATATAATATTGTCAAAGAAGGATCCAAACTACGAAGCCACTAGGCTTCATTTTGAAAATCTTGCCAAGAGATATGGGAACCCCATAATTATTTTAAATCTAATCAAGACACAGGAAAAAAGGCCTCGAGAGTCCATTCTACGCACAGAGTTTGCTAATGCAATTGATTTCATTAATAAAGATCTATCTGAGGAGAACCATCTCAGATTTCTCCACTGGGATTTGCACAAACATTCTCGAAGCAAAGCTATAAATGCTTTACTACTCTTGGGAAAGGTGGCTACATATGCCTTGACTTTAACTGGGTTCTTCTATTGTCAAGTTACACCAGAAATGGGACCTGAGGGCATGATTAATTGGCCTTCATCGGAGAATTTTGACAATTGTATGTCTCCCCAAAGACATTGTGCTAATGGAGATGAGGATGTTGATAGCATAGTGGGGAAACCAACTGGAGGCAGCAACATTGCAAATGGAAATCATTCTAGCAAACCAGCCATATTCCAGAATGGGGTGCTGAGGACCAATTGCATAGACTGCCTTGATCGGACAAATGTTGCACAGTATGCATATGGGTTGGCTGCTCTAGGACACCAGCTTCATGCTTTGGGAGTTTTAGAGAATCCCAAGATAGACCTTGACGATCCATTGGCTGATGATTTAATGGGATTTTATGAAAGAATGGGTGACACACTTGCACACCAGTATGGTGGTTCAGCTGCTCATAATAAGATATTTTCTGAGAGAAGAGGTCAGTGGAGAGCAGCAACTCAGTCTCAGGAATTCTTCAGAACTCTTCAACGGTATTACAGCAATGCGTATATGGATGCACAGAAGCAAGATGCAATTAATATATTTCTTGGGTATTTTCAACCACAGCAAGATAAACCTGCACTCTGGGAACTGGATTCAGACCAGCATTACCATGATGGGAGGCATGGGTCAAAAGACACGGATGAAGATGGATGGTTCGGTTTCAAGAGATCATATTCCGATGGAAACATTCTCCGTCCAAATAGCTCACCTATGTCAGCACCAACTGTTAAGCAGGAGAAATTTACTAAATATCCGGTTTTGCCTGATCAAACTCAACAAGGCAAGACACTTTCAGAATCCTCACCTGAAATATCAACTTGTGAGAGTGATATTGCATATTCAAGGTACACTCCCTCGATGCCTCGCAGACAACTTTTTGGAGAGTTGCAAAGATATCGGTGTCTTGAAAGTGATCATATTTACTATTCTGAACATGGAGATGGGTATAATTTCTCAAACTTTGTGGATCTAGACTGGCTATCTTCCTCTGGGAATTCATGCGAGGAGGAGCTGTATGAGAGATCGTCAATACTTACCAACTCACCATTTGAGGGGCTATCATCGGAAAATGTTGTCAACGGAATAATAGGAGATTCTACACCCTCCACAAGTGTGTATGGATCCATCTTGAAGGTCAGTAAACTTTCCTTTGAAATGCTAGAAGTAATTTCCCACTTGATAGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005811 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0009506 GO:0009653 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031982 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034596 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098805 GO:0120035 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

824

Amino Acids

94.0

Weight (kDa)

6.29

Isoelectric Point (pI)

51.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 106 - 404 8.6e-83 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 368
AarI CACCTGC 1 cut(s) 675
AasI GACNNNNNNGTC 1 cut(s) 774
Acc36I ACCTGC 2 cut(s) 675, 1870
AccB1I GGYRCC 1 cut(s) 389
AccII CGCG 1 cut(s) 955
AciI CCGC 5 cut(s) 32, 262, 291, 407, 785
AclWI GGATC 9 cut(s) 185, 198, 430, 989, 1002, 1610, 2262, 2403, 2416
AcoI YGGCCR 1 cut(s) 400
AcsI RAATTY 7 cut(s) 98, 338, 448, 1345, 1754, 2027, 2281
AcuI CTGAAG 1 cut(s) 1744
AfaI GTAC 4 cut(s) 171, 267, 909, 2135
AfiI CCNNNNNNNGG 5 cut(s) 529, 1307, 1427, 1483, 2339
AflIII ACRYGT 2 cut(s) 582, 604
AjiI CACGTC 2 cut(s) 740, 776
AjnI CCWGG 1 cut(s) 791
AleI CACNNNNGTG 1 cut(s) 2399
Alw21I GWGCWC 2 cut(s) 204, 515
Alw26I GTCTC 6 cut(s) 48, 107, 138, 839, 1368, 1752
Alw44I GTGCAC 1 cut(s) 511
AlwI GGATC 9 cut(s) 185, 198, 430, 989, 1002, 1610, 2262, 2403, 2416
Ama87I CYCGRG 2 cut(s) 648, 1098
AoxI GGCC 6 cut(s) 3, 347, 400, 717, 1094, 1331
ApaI GGGCCC 1 cut(s) 351
ApaLI GTGCAC 1 cut(s) 511
ApeKI GCWGC 6 cut(s) 28, 361, 1431, 1553, 1691, 1736
ApoI RAATTY 7 cut(s) 98, 338, 448, 1345, 1754, 2027, 2281
AseI ATTAAT 3 cut(s) 1146, 1326, 1821
Asp700I GAANNNNTTC 2 cut(s) 638, 1453
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 5 cut(s) 347, 348, 520, 1310, 1494
AsuC2I CCSGG 1 cut(s) 48
AsuHPI GGTGA 6 cut(s) 444, 656, 1670, 1986, 2082, 2322
AvaI CYCGRG 2 cut(s) 648, 1098
AvaII GGWCC 3 cut(s) 520, 1310, 1494
AxyI CCTNAGG 2 cut(s) 876, 1314
BaeGI GKGCMC 2 cut(s) 351, 515
BalI TGGCCA 1 cut(s) 402
BamHI GGATCC 3 cut(s) 190, 994, 2408
BanI GGYRCC 1 cut(s) 389
BanII GRGCYC 2 cut(s) 204, 351
BbsI GAAGAC 2 cut(s) 495, 563
Bbv12I GWGCWC 2 cut(s) 204, 515
BbvCI CCTCAGC 2 cut(s) 78, 1490
BbvI GCAGC 6 cut(s) 40, 348, 1443, 1540, 1678, 1748
BccI CCATC 9 cut(s) 425, 592, 1179, 1895, 1928, 1932, 1961, 2222, 2420
BceAI ACGGC 2 cut(s) 19, 334
BcgI CGANNNNNNTGC 2 cut(s) 2165, 2199
BciT130I CCWGG 1 cut(s) 793
BclI TGATCA 3 cut(s) 589, 2053, 2200
BcnI CCSGG 1 cut(s) 48
BcoDI GTCTC 6 cut(s) 48, 107, 138, 839, 1368, 1752
BfaI CTAG 6 cut(s) 480, 1014, 1458, 1559, 2258, 2447
BfuAI ACCTGC 2 cut(s) 675, 1870
BglII AGATCT 1 cut(s) 1153
BisI GCNGC 7 cut(s) 29, 32, 362, 1432, 1554, 1692, 1737
BlsI GCNGC 7 cut(s) 30, 33, 363, 1433, 1555, 1693, 1738
Bme1390I CCNGG 2 cut(s) 48, 793
Bme18I GGWCC 3 cut(s) 520, 1310, 1494
BmeT110I CYCGRG 2 cut(s) 648, 1098
BmgBI CACGTC 2 cut(s) 740, 776
BmgT120I GGNCC 5 cut(s) 347, 348, 520, 1310, 1494
BmiI GGNNCC 9 cut(s) 192, 348, 349, 391, 790, 996, 1052, 1311, 2410
BmrFI CCNGG 2 cut(s) 48, 793
BmrI ACTGGG 2 cut(s) 1198, 1284
BmsI GCATC 3 cut(s) 1789, 1804, 2136
BmuI ACTGGG 2 cut(s) 1198, 1284
BpiI GAAGAC 2 cut(s) 495, 563
BpmI CTGGAG 1 cut(s) 1446
Bpu10I CCTNAGC 2 cut(s) 78, 1490
BpuEI CTTGAG 2 cut(s) 233, 277
BpuMI CCSGG 1 cut(s) 48
Bsa29I ATCGAT 1 cut(s) 184
BsaI GGTCTC 1 cut(s) 839
BsaJI CCNNGG 2 cut(s) 46, 916
BsaWI WCCGGW 1 cut(s) 2041
BsaXI ACNNNNNCTCC 2 cut(s) 2285, 2315
Bsc4I CCNNNNNNNGG 5 cut(s) 529, 1307, 1427, 1483, 2339
Bse1I ACTGG 7 cut(s) 292, 1193, 1279, 1429, 1675, 1881, 2267
Bse21I CCTNAGG 2 cut(s) 876, 1314
Bse3DI GCAATG 3 cut(s) 759, 1437, 1792
BseBI CCWGG 1 cut(s) 793
BseCI ATCGAT 1 cut(s) 184
BseDI CCNNGG 2 cut(s) 46, 916
BseGI GGATG 5 cut(s) 874, 1402, 1804, 1933, 1943
BseLI CCNNNNNNNGG 5 cut(s) 529, 1307, 1427, 1483, 2339
BseMI GCAATG 3 cut(s) 759, 1437, 1792
BseNI ACTGG 7 cut(s) 292, 1193, 1279, 1429, 1675, 1881, 2267
BseRI GAGGAG 6 cut(s) 63, 66, 66, 1178, 2306, 2309
BseSI GKGCMC 2 cut(s) 351, 515
BseXI GCAGC 6 cut(s) 40, 348, 1443, 1540, 1678, 1748
BsgI GTGCAG 2 cut(s) 484, 1848
Bsh1236I CGCG 1 cut(s) 955
Bsh1285I CGRYCG 1 cut(s) 122
BshFI GGCC 6 cut(s) 5, 349, 402, 719, 1096, 1333
BshNI GGYRCC 1 cut(s) 389
BshVI ATCGAT 1 cut(s) 184
BsiEI CGRYCG 1 cut(s) 122
BsiHKAI GWGCWC 2 cut(s) 204, 515
BsiHKCI CYCGRG 2 cut(s) 648, 1098
BsiSI CCGG 3 cut(s) 23, 48, 2042
BslFI GGGAC 2 cut(s) 615, 1323
BslI CCNNNNNNNGG 5 cut(s) 529, 1307, 1427, 1483, 2339
BsmAI GTCTC 6 cut(s) 48, 107, 138, 839, 1368, 1752
BsmBI CGTCTC 1 cut(s) 48
BsmFI GGGAC 2 cut(s) 615, 1323
BsnI GGCC 6 cut(s) 5, 349, 402, 719, 1096, 1333
Bso31I GGTCTC 1 cut(s) 839
BsoBI CYCGRG 2 cut(s) 648, 1098
Bsp120I GGGCCC 1 cut(s) 347
Bsp1286I GDGCHC 3 cut(s) 204, 351, 515
Bsp1407I TGTACA 1 cut(s) 907
BspACI CCGC 5 cut(s) 32, 262, 291, 407, 785
BspANI GGCC 6 cut(s) 5, 349, 402, 719, 1096, 1333
BspDI ATCGAT 1 cut(s) 184
BspFNI CGCG 1 cut(s) 955
BspLI GGNNCC 9 cut(s) 192, 348, 349, 391, 790, 996, 1052, 1311, 2410
BspMI ACCTGC 2 cut(s) 675, 1870
BspPI GGATC 9 cut(s) 185, 198, 430, 989, 1002, 1610, 2262, 2403, 2416
BspT107I GGYRCC 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 839
BsrDI GCAATG 3 cut(s) 759, 1437, 1792
BsrGI TGTACA 1 cut(s) 907
BsrI ACTGG 7 cut(s) 292, 1193, 1279, 1429, 1675, 1881, 2267
BssECI CCNNGG 2 cut(s) 46, 916
Bst2UI CCWGG 1 cut(s) 793
Bst4CI ACNGT 6 cut(s) 679, 688, 917, 1536, 1776, 2014
Bst6I CTCTTC 4 cut(s) 136, 820, 1714, 1773
BstAPI GCANNNNNTGC 2 cut(s) 1509, 1538
BstAUI TGTACA 1 cut(s) 907
BstDSI CCRYGG 1 cut(s) 916
BstENI CCTNNNNNAGG 1 cut(s) 527
BstF5I GGATG 5 cut(s) 874, 1402, 1804, 1933, 1943
BstFNI CGCG 1 cut(s) 955
BstHHI GCGC 1 cut(s) 22
BstMAI GTCTC 6 cut(s) 48, 107, 138, 839, 1368, 1752
BstMCI CGRYCG 1 cut(s) 122
BstMWI GCNNNNNNNGC 6 cut(s) 28, 413, 1226, 1259, 1509, 1538
BstNI CCWGG 1 cut(s) 793
BstNSI RCATGY 3 cut(s) 361, 586, 608
BstSCI CCNGG 2 cut(s) 46, 791
BstSLI GKGCMC 2 cut(s) 351, 515
BstUI CGCG 1 cut(s) 955
BstV1I GCAGC 6 cut(s) 40, 348, 1443, 1540, 1678, 1748
BstV2I GAAGAC 2 cut(s) 495, 563
BstX2I RGATCY 5 cut(s) 190, 994, 1153, 2254, 2408
BstXI CCANNNNNNTGG 1 cut(s) 1682
BstYI RGATCY 5 cut(s) 190, 994, 1153, 2254, 2408
Bsu15I ATCGAT 1 cut(s) 184
Bsu36I CCTNAGG 2 cut(s) 876, 1314
BsuRI GGCC 6 cut(s) 5, 349, 402, 719, 1096, 1333
BsuTUI ATCGAT 1 cut(s) 184
BtgI CCRYGG 1 cut(s) 916
BtgZI GCGATG 1 cut(s) 936
BtrI CACGTC 2 cut(s) 740, 776
BtsCI GGATG 5 cut(s) 874, 1402, 1804, 1933, 1943
BtsIMutI CAGTG 5 cut(s) 211, 285, 467, 1186, 1733
BveI ACCTGC 2 cut(s) 675, 1870
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 5 cut(s) 347, 348, 520, 1310, 1494
ClaI ATCGAT 1 cut(s) 184
CsiI ACCWGGT 1 cut(s) 791
Csp6I GTAC 4 cut(s) 170, 266, 908, 2134
CspCI CAANNNNNGTGG 2 cut(s) 2232, 2267
CviQI GTAC 4 cut(s) 170, 266, 908, 2134
DraI TTTAAA 2 cut(s) 960, 1068
DrdI GACNNNNNNGTC 1 cut(s) 774
DseDI GACNNNNNNGTC 1 cut(s) 774
EaeI YGGCCR 1 cut(s) 400
Eam1104I CTCTTC 4 cut(s) 136, 820, 1714, 1773
EarI CTCTTC 4 cut(s) 136, 820, 1714, 1773
EciI GGCGGA 1 cut(s) 306
Ecl136II GAGCTC 1 cut(s) 202
Eco147I AGGCCT 1 cut(s) 1096
Eco24I GRGCYC 2 cut(s) 204, 351
Eco31I GGTCTC 1 cut(s) 839
Eco32I GATATC 1 cut(s) 2183
Eco47I GGWCC 3 cut(s) 520, 1310, 1494
Eco53kI GAGCTC 1 cut(s) 202
Eco57I CTGAAG 1 cut(s) 1744
Eco81I CCTNAGG 2 cut(s) 876, 1314
Eco88I CYCGRG 2 cut(s) 648, 1098
EcoICRI GAGCTC 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 527
EcoO109I RGGNCCY 3 cut(s) 347, 348, 1310
EcoRI GAATTC 2 cut(s) 1754, 2281
EcoRII CCWGG 1 cut(s) 791
EcoRV GATATC 1 cut(s) 2183
EcoT22I ATGCAT 1 cut(s) 1543
EcoT38I GRGCYC 2 cut(s) 204, 351
Esp3I CGTCTC 1 cut(s) 48
FalI AAGNNNNNCTT 2 cut(s) 521, 553
FaqI GGGAC 2 cut(s) 615, 1323
FauI CCCGC 2 cut(s) 255, 778
FauNDI CATATG 2 cut(s) 1258, 1543
FbaI TGATCA 3 cut(s) 589, 2053, 2200
Fnu4HI GCNGC 7 cut(s) 29, 32, 362, 1432, 1554, 1692, 1737
FokI GGATG 5 cut(s) 881, 1409, 1811, 1940, 1950
FriOI GRGCYC 2 cut(s) 204, 351
Fsp4HI GCNGC 7 cut(s) 29, 32, 362, 1432, 1554, 1692, 1737
FspBI CTAG 6 cut(s) 480, 1014, 1458, 1559, 2258, 2447
GlaI GCGC 1 cut(s) 21
GluI GCNGC 7 cut(s) 29, 32, 362, 1432, 1554, 1692, 1737
GsuI CTGGAG 1 cut(s) 1446
HaeIII GGCC 6 cut(s) 5, 349, 402, 719, 1096, 1333
HapII CCGG 3 cut(s) 23, 48, 2042
HhaI GCGC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 1 cut(s) 2365
HindII GTYRAC 1 cut(s) 2365
HinfI GANTC 9 cut(s) 115, 218, 476, 661, 1103, 1594, 1880, 2084, 2381
HpaII CCGG 3 cut(s) 23, 48, 2042
HphI GGTGA 6 cut(s) 444, 656, 1670, 1986, 2082, 2322
Hpy166II GTNNAC 5 cut(s) 513, 773, 2136, 2365, 2429
Hpy8I GTNNAC 5 cut(s) 513, 773, 2136, 2365, 2429
Hpy99I CGWCG 1 cut(s) 13
HpyAV CCTTC 5 cut(s) 266, 814, 985, 1344, 2413
HpyCH4III ACNGT 6 cut(s) 679, 688, 917, 1536, 1776, 2014
HpyCH4IV ACGT 6 cut(s) 158, 238, 739, 769, 775, 805
HpyF10VI GCNNNNNNNGC 6 cut(s) 28, 413, 1226, 1259, 1509, 1538
HpySE526I ACGT 6 cut(s) 158, 238, 739, 769, 775, 805
HspAI GCGC 1 cut(s) 20
Ksp22I TGATCA 3 cut(s) 589, 2053, 2200
LmnI GCTCC 3 cut(s) 25, 510, 2296
Lsp1109I GCAGC 6 cut(s) 40, 348, 1443, 1540, 1678, 1748
LweI GCATC 3 cut(s) 1789, 1804, 2136
MabI ACCWGGT 1 cut(s) 791
MaeI CTAG 6 cut(s) 480, 1014, 1458, 1559, 2258, 2447
MaeII ACGT 6 cut(s) 158, 238, 739, 769, 775, 805
MaeIII GTNAC 8 cut(s) 283, 304, 314, 740, 776, 939, 1294, 1658
MfeI CAATTG 3 cut(s) 1134, 1354, 1498
MflI RGATCY 5 cut(s) 190, 994, 1153, 2254, 2408
MhlI GDGCHC 3 cut(s) 204, 351, 515
MlsI TGGCCA 1 cut(s) 402
MluNI TGGCCA 1 cut(s) 402
MlyI GAGTC 2 cut(s) 109, 1112
Mox20I TGGCCA 1 cut(s) 402
Mph1103I ATGCAT 1 cut(s) 1543
MroXI GAANNNNTTC 2 cut(s) 638, 1453
MscI TGGCCA 1 cut(s) 402
MslI CAYNNNNRTG 5 cut(s) 89, 1677, 1896, 2226, 2399
Msp20I TGGCCA 1 cut(s) 402
MspA1I CMGCKG 1 cut(s) 1691
MspI CCGG 3 cut(s) 23, 48, 2042
MspR9I CCNGG 2 cut(s) 48, 793
MssI GTTTAAAC 1 cut(s) 960
MunI CAATTG 3 cut(s) 1134, 1354, 1498
MvaI CCWGG 1 cut(s) 793
MvnI CGCG 1 cut(s) 955
MwoI GCNNNNNNNGC 6 cut(s) 28, 413, 1226, 1259, 1509, 1538
NciI CCSGG 1 cut(s) 48
NdeI CATATG 2 cut(s) 1258, 1543
NlaIV GGNNCC 9 cut(s) 192, 348, 349, 391, 790, 996, 1052, 1311, 2410
NmuCI GTSAC 6 cut(s) 283, 304, 740, 776, 939, 1658
NsiI ATGCAT 1 cut(s) 1543
NspI RCATGY 3 cut(s) 361, 586, 608
OliI CACNNNNGTG 1 cut(s) 2399
PaeR7I CTCGAG 1 cut(s) 1098
PaqCI CACCTGC 1 cut(s) 675
PceI AGGCCT 1 cut(s) 1096
PciI ACATGT 2 cut(s) 582, 604
PdmI GAANNNNTTC 2 cut(s) 638, 1453
PfeI GAWTC 7 cut(s) 218, 476, 661, 1594, 1880, 2084, 2381
PkrI GCNGC 7 cut(s) 30, 33, 363, 1433, 1555, 1693, 1738
Ple19I CGATCG 1 cut(s) 122
PleI GAGTC 2 cut(s) 109, 1111
PmeI GTTTAAAC 1 cut(s) 960
PpsI GAGTC 2 cut(s) 109, 1111
PpuMI RGGWCCY 1 cut(s) 1310
PscI ACATGT 2 cut(s) 582, 604
PshBI ATTAAT 3 cut(s) 1146, 1326, 1821
PsiI TTATAA 1 cut(s) 368
Psp124BI GAGCTC 1 cut(s) 204
Psp5II RGGWCCY 1 cut(s) 1310
Psp6I CCWGG 1 cut(s) 791
PspGI CCWGG 1 cut(s) 791
PspN4I GGNNCC 9 cut(s) 192, 348, 349, 391, 790, 996, 1052, 1311, 2410
PspOMI GGGCCC 1 cut(s) 347
PspPI GGNCC 5 cut(s) 347, 348, 520, 1310, 1494
PspPPI RGGWCCY 1 cut(s) 1310
PsuI RGATCY 5 cut(s) 190, 994, 1153, 2254, 2408
PvuI CGATCG 1 cut(s) 122
PvuII CAGCTG 1 cut(s) 1691
RsaI GTAC 4 cut(s) 171, 267, 909, 2135
RsaNI GTAC 4 cut(s) 170, 266, 908, 2134
RseI CAYNNNNRTG 5 cut(s) 89, 1677, 1896, 2226, 2399
SacI GAGCTC 1 cut(s) 204
SatI GCNGC 7 cut(s) 29, 32, 362, 1432, 1554, 1692, 1737
Sau96I GGNCC 5 cut(s) 347, 348, 520, 1310, 1494
SchI GAGTC 2 cut(s) 109, 1112
ScrFI CCNGG 2 cut(s) 48, 793
SduI GDGCHC 3 cut(s) 204, 351, 515
SexAI ACCWGGT 1 cut(s) 791
SfaNI GCATC 3 cut(s) 1789, 1804, 2136
Sfr274I CTCGAG 1 cut(s) 1098
SinI GGWCC 3 cut(s) 520, 1310, 1494
SlaI CTCGAG 1 cut(s) 1098
SmiMI CAYNNNNRTG 5 cut(s) 89, 1677, 1896, 2226, 2399
SmlI CTYRAG 3 cut(s) 212, 256, 1098
SmoI CTYRAG 3 cut(s) 212, 256, 1098
SseBI AGGCCT 1 cut(s) 1096
SsiI CCGC 5 cut(s) 32, 262, 291, 407, 785
SspI AATATT 1 cut(s) 981
SspMI CTAG 6 cut(s) 480, 1014, 1458, 1559, 2258, 2447
SstI GAGCTC 1 cut(s) 204
StuI AGGCCT 1 cut(s) 1096
StyD4I CCNGG 2 cut(s) 46, 791
TaaI ACNGT 6 cut(s) 679, 688, 917, 1536, 1776, 2014
TaiI ACGT 6 cut(s) 161, 241, 742, 772, 778, 808
TaqI TCGA 6 cut(s) 118, 122, 184, 1099, 1210, 2144
TaqII GACCGA 1 cut(s) 863
TatI WGTACW 1 cut(s) 907
TauI GCSGC 1 cut(s) 34
TfiI GAWTC 7 cut(s) 218, 476, 661, 1594, 1880, 2084, 2381
TscAI CASTG 5 cut(s) 211, 292, 467, 1193, 1733
TseFI GTSAC 6 cut(s) 283, 304, 740, 776, 939, 1658
TseI GCWGC 6 cut(s) 28, 361, 1431, 1553, 1691, 1736
Tsp45I GTSAC 6 cut(s) 283, 304, 740, 776, 939, 1658
TspGWI ACGGA 3 cut(s) 1940, 1970, 2382
TspRI CASTG 5 cut(s) 211, 292, 467, 1193, 1733
VneI GTGCAC 1 cut(s) 511
VpaK11BI GGWCC 3 cut(s) 520, 1310, 1494
VspI ATTAAT 3 cut(s) 1146, 1326, 1821
XagI CCTNNNNNAGG 1 cut(s) 527
XapI RAATTY 7 cut(s) 98, 338, 448, 1345, 1754, 2027, 2281
XbaI TCTAGA 2 cut(s) 479, 2257
XceI RCATGY 3 cut(s) 361, 586, 608
XhoI CTCGAG 1 cut(s) 1098
XmnI GAANNNNTTC 2 cut(s) 638, 1453
XspI CTAG 6 cut(s) 480, 1014, 1458, 1559, 2258, 2447
Zsp2I ATGCAT 1 cut(s) 1543
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.