MD02G1102300.v1.1

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007042
Physical Location & Seq
Reverse (-)
8203276 .. 8211475
8200 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1102300.v1.1.491

Sequence Viewer

Length: 2538 bp
ATGGCGTCCTCAGAGAATGAGCCTATGTCTCAATTGGGTGGCGGCGAAACTCGGGCTCCTTCCCAACACGTAGATATGCAGAAATTCCGGCTTTACGAGACACGAGCGAACTTTTACATGATCGGGAGAGATAAGAGTAGAACGTATTGGAGAGTACTAAAGATTGACCGGTTGGACCCTTGTGAGCTTAACATTCGTGAAGATTCTACCACGTACACTGAAAGAGAATGTTCTGACCTGTTGTGGCGGATACATGAAGGGAATAAGGCTACCGGTGGATTGAAGTTTGTCACCAATTGTTATGGAATTGTTGGATTCGTTAAATTTTTAGGGCCCTATTACATGCTGCTTATCACGAAAAGAAGACAAATAGGCGCAATCTGTGGTCATACTGTATATGCAGTCTCCAAGAGTGAAATGATACCGCTTCTGAATTCTTCTGTCCAATCCAGTACTACTAATTCTAAAAATGAAAACAGATACAAGAAGCTCTTATGCACAGTGGACCTTACAAAGGACTTCTTTTTCAGCTACTCATACCATGTTATGCGTAGTCTGCAAAAGAACATGTGTAAGAATGAGACAGGAAATGTCCTTTATGAAACCATGTTTGTCTGGAATGAGTTTTTAACTCGGGGAATTCGGAATCACCTCCAAAATACTCTTTGGACGGTTGCATTGGTTTATGGCTTCTTTAAACAGGCCACTCTCTCTATATCTGGACGTGACTTCAAGCTGACCCTTATTGCAAGACGTTCACGTCATTATGCAGGCACCAGGTATCTGAAACGTGGTGTTAATGAAGAGGGAAGAGTAGCTAATGACGTTGAGACAGAACAGATTGTCTGTGAGGATGTTCCTGAGGGGTTTCCGATGCAGATAAGTTCTGTTGTGCAGAACCGGGGCTCAATCCCTCTATTTTGGTCACAGGAAACCTCACGTTTAAATATCAAACCAGATATAATATTGTCAAAAAAGGACCAAAACTATGAAGCCACTAGGCTGCACTTTGAAAATCTTGTTAAGAGATATGGGAACCCCATTATTATATTGAATCTGATCAAGACACAAGAGAAGAGGCCTCGAGAATCGATACTACGTAGAGAGTTTGCTAATGCAATTGATTTCATTAATAAAGACCTATCTGATGAGAACCGTCTGAAATTCCTTCACTGGGACCTCCACAAACATCCCCGGAGCAAAGCTACGGATGCTTTACTACTCTTGGGCAAGGTGGCTACATATGCATTGACACTAACTGGGTTCTTCTATTGTCAAGTTAACCCAGCACTGAGACCTGACGGTGTCATTAAGTGGCCTTCCTCTGGGCATTTCGACAGTAACTTGTTTCCCGAAATACATAATGCTAATGGTGATGGGGAAGCTAATGGTAATGGGGATGCTGATAACATAGAGTGTAAACCAAGTGGAGGCAGAAATATTGCAAATGGTAATCATTCTGTCAAGCCATCCATGTTCCAGAGCGGGGTGCTTAGAACCAATTGCATAGACTGTCTGGATCGCACAAATGTTGCTCAGTATGCATATGGGTTGGCTGCATTGGGACACCAGCTTCATGCTTTGGGAGCTATAGACAATCCAAGGATAGATCTTGATGCTACTTTGGCTGAAGATTTGATGGGATTTTACGAAAGAATGGGTGACACACTTGCACACCAGTATGGTGGTTCCGCTGCACATAATAAGATGTTTTCTGAGAGAAGGGGTCAGTGGAGAGCAGCAACTCAGTCTCAGGAATTCTTTAGAACTCTTCAACGGTATTACAGCAATGCTTACATGGATACAGAGAAGCAAGATGCAATTAATGTATTTCTAGGGCATTTTCAACCACAACAAGGCAAACCTGCACTGTGGGAATTGGATTCTGACCAGAATTTTTACTCCGGGAAGGATGAACCAAAAGATAAGGATCTAGATGGAAGGTCCACTTTCAAAAGATCTTATTCCGATGGCAACATTCTTCAAGAAAGCAGCTCACTTATGTCATCCCCACATGTTAAGCAGGAGAAATTTACAAACCCTTGTATGCCTGATCGATCACAACAAGAAAGCAAGATTCTTTCAGAGTCATCACCTGATATATCAACTTCTGAGAGTGATATTGCATTTTCAAGGTACACTCCCTCGATGCCTCGCAGGCAACTTTTTGGAGAGTTTCAAAGATACCGGTGTGAAAGTGAGCATATTTACTATTCCGAACGTGGAGATACGTACAACTTCTCAAACTTTGTTGACCTGGACTGGCTATCTTCATCTGGGAATTCTTGTGAGGAGGAGCCATTTGAGAGGTCGTCAGTACTTGCATCTCCAATTGAGGGGCTATCAGCGGAGAATGTTGTTAACGGAATAATTGGGGATTCTACTCCATCTACTAGTGAATATGGATCAAGCATGAAGGGGAAGGAGCAGGCAGGAACGGGGTTGTCCTTTCATAACGGGGAGAGTTCCAACGTTCTCGAGGAGTATCCCGATAGCTTTGTGAACTGGGTCAACTTCGGAGAGACGATATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005811 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0009506 GO:0009653 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031982 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034596 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098805 GO:0120035 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

846

Amino Acids

96.11

Weight (kDa)

6.3

Isoelectric Point (pI)

50.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 101 - 397 2.7e-84 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 759
Acc36I ACCTGC 1 cut(s) 1873
AccB1I GGYRCC 1 cut(s) 773
AccBSI CCGCTC 1 cut(s) 1485
AciI CCGC 6 cut(s) 42, 247, 425, 1485, 1692, 2348
AclI AACGTT 1 cut(s) 2472
AclWI GGATC 3 cut(s) 1527, 1938, 2413
AcsI RAATTY 9 cut(s) 83, 323, 433, 639, 1163, 1757, 1894, 2030, 2281
AcuI CTGAAG 1 cut(s) 1650
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 6 cut(s) 156, 215, 454, 2138, 2234, 2319
AfiI CCNNNNNNNGG 7 cut(s) 514, 1174, 1325, 1430, 1486, 1856, 2336
AflIII ACRYGT 3 cut(s) 67, 567, 2014
AgeI ACCGGT 3 cut(s) 168, 272, 2187
AhlI ACTAGT 1 cut(s) 2393
AjiI CACGTC 2 cut(s) 725, 761
AjnI CCWGG 2 cut(s) 776, 2256
Alw26I GTCTC 8 cut(s) 33, 92, 409, 575, 824, 1288, 1755, 2516
AlwI GGATC 3 cut(s) 1527, 1938, 2413
Ama87I CYCGRG 4 cut(s) 51, 633, 1083, 2477
AoxI GGCC 4 cut(s) 332, 702, 1079, 1316
ApaI GGGCCC 1 cut(s) 336
ApeKI GCWGC 6 cut(s) 346, 1003, 1556, 1694, 1739, 1992
ApoI RAATTY 9 cut(s) 83, 323, 433, 639, 1163, 1757, 1894, 2030, 2281
AseI ATTAAT 2 cut(s) 1131, 1824
AsiGI ACCGGT 3 cut(s) 168, 272, 2187
AspLEI GCGC 1 cut(s) 377
AspS9I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1177, 1944
AsuC2I CCSGG 3 cut(s) 902, 1195, 1906
AsuHPI GGTGA 5 cut(s) 283, 641, 1385, 1673, 2085
AvaI CYCGRG 4 cut(s) 51, 633, 1083, 2477
AvaII GGWCC 5 cut(s) 175, 505, 979, 1177, 1944
AxyI CCTNAGG 1 cut(s) 861
BaeGI GKGCMC 1 cut(s) 336
BaeI ACNNNNGTAYC 2 cut(s) 2176, 2209
BanI GGYRCC 1 cut(s) 773
BanII GRGCYC 3 cut(s) 58, 336, 908
BauI CACGAG 1 cut(s) 102
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 6 cut(s) 333, 990, 1543, 1681, 1751, 2004
BccI CCATC 6 cut(s) 1370, 1477, 1633, 1931, 1964, 2395
BciT130I CCWGG 2 cut(s) 778, 2258
BciVI GTATCC 3 cut(s) 243, 1795, 2496
BclI TGATCA 1 cut(s) 1059
BcnI CCSGG 3 cut(s) 902, 1195, 1906
BcoDI GTCTC 8 cut(s) 33, 92, 409, 575, 824, 1288, 1755, 2516
BcuI ACTAGT 1 cut(s) 2393
BfaI CTAG 4 cut(s) 999, 1835, 1934, 2394
BfmI CTRYAG 1 cut(s) 1590
BfuAI ACCTGC 1 cut(s) 1873
BfuI GTATCC 3 cut(s) 243, 1795, 2496
BglI GCCNNNNNGGC 1 cut(s) 2158
BglII AGATCT 2 cut(s) 1609, 1958
BisI GCNGC 7 cut(s) 43, 347, 1004, 1557, 1695, 1740, 1993
BlsI GCNGC 7 cut(s) 44, 348, 1005, 1558, 1696, 1741, 1994
BmcAI AGTACT 3 cut(s) 156, 454, 2319
Bme1390I CCNGG 5 cut(s) 778, 902, 1195, 1906, 2258
Bme18I GGWCC 5 cut(s) 175, 505, 979, 1177, 1944
BmeT110I CYCGRG 4 cut(s) 51, 633, 1083, 2477
BmgBI CACGTC 2 cut(s) 725, 761
BmgT120I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1177, 1944
BmiI GGNNCC 8 cut(s) 57, 177, 334, 775, 1037, 1178, 1690, 2298
BmrFI CCNGG 5 cut(s) 778, 902, 1195, 1906, 2258
BmrI ACTGGG 3 cut(s) 1183, 1269, 2515
BmsI GCATC 7 cut(s) 864, 1201, 1390, 1606, 1807, 2139, 2333
BmuI ACTGGG 3 cut(s) 1183, 1269, 2515
BpiI GAAGAC 1 cut(s) 370
BpuMI CCSGG 3 cut(s) 902, 1195, 1906
Bsa29I ATCGAT 2 cut(s) 1091, 2056
BsaAI YACGTR 4 cut(s) 70, 213, 1100, 2232
BsaBI GATNNNNATC 1 cut(s) 1929
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 1288
BsaJI CCNNGG 3 cut(s) 901, 1193, 1601
BsaWI WCCGGW 3 cut(s) 168, 272, 2187
BsaXI ACNNNNNCTCC 2 cut(s) 40, 70
Bsc4I CCNNNNNNNGG 7 cut(s) 514, 1174, 1325, 1430, 1486, 1856, 2336
Bse118I RCCGGY 3 cut(s) 168, 272, 2187
Bse1I ACTGG 6 cut(s) 450, 1178, 1264, 1678, 2267, 2510
Bse21I CCTNAGG 1 cut(s) 861
Bse3DI GCAATG 1 cut(s) 1795
Bse8I GATNNNNATC 1 cut(s) 1929
BseBI CCWGG 2 cut(s) 778, 2258
BseCI ATCGAT 2 cut(s) 1091, 2056
BseDI CCNNGG 3 cut(s) 901, 1193, 1601
BseGI GGATG 7 cut(s) 859, 1189, 1216, 1405, 1469, 1918, 2006
BseJI GATNNNNATC 1 cut(s) 1929
BseLI CCNNNNNNNGG 7 cut(s) 514, 1174, 1325, 1430, 1486, 1856, 2336
BseMI GCAATG 1 cut(s) 1795
BseMII CTCAG 8 cut(s) 24, 852, 1283, 1550, 1707, 1760, 1766, 2103
BseNI ACTGG 6 cut(s) 450, 1178, 1264, 1678, 2267, 2510
BseRI GAGGAG 3 cut(s) 2306, 2309, 2495
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 6 cut(s) 333, 990, 1543, 1681, 1751, 2004
BseYI CCCAGC 1 cut(s) 1285
BsgI GTGCAG 4 cut(s) 914, 989, 1680, 1851
BshFI GGCC 4 cut(s) 334, 704, 1081, 1318
BshNI GGYRCC 1 cut(s) 773
BshTI ACCGGT 3 cut(s) 168, 272, 2187
BshVI ATCGAT 2 cut(s) 1091, 2056
BsiHKCI CYCGRG 4 cut(s) 51, 633, 1083, 2477
BsiSI CCGG 7 cut(s) 88, 169, 273, 901, 1195, 1905, 2188
BslFI GGGAC 2 cut(s) 1190, 1578
BslI CCNNNNNNNGG 7 cut(s) 514, 1174, 1325, 1430, 1486, 1856, 2336
BsmAI GTCTC 8 cut(s) 33, 92, 409, 575, 824, 1288, 1755, 2516
BsmBI CGTCTC 1 cut(s) 2516
BsmFI GGGAC 2 cut(s) 1190, 1578
BsnI GGCC 4 cut(s) 334, 704, 1081, 1318
Bso31I GGTCTC 1 cut(s) 1288
BsoBI CYCGRG 4 cut(s) 51, 633, 1083, 2477
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 3 cut(s) 58, 336, 908
Bsp143I GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
BspACI CCGC 6 cut(s) 42, 247, 425, 1485, 1692, 2348
BspANI GGCC 4 cut(s) 334, 704, 1081, 1318
BspCNI CTCAG 8 cut(s) 23, 853, 1284, 1549, 1708, 1759, 1765, 2104
BspDI ATCGAT 2 cut(s) 1091, 2056
BspLI GGNNCC 8 cut(s) 57, 177, 334, 775, 1037, 1178, 1690, 2298
BspMI ACCTGC 1 cut(s) 1873
BspPI GGATC 3 cut(s) 1527, 1938, 2413
BspT107I GGYRCC 1 cut(s) 773
BspTNI GGTCTC 1 cut(s) 1288
BsrBI CCGCTC 1 cut(s) 1485
BsrDI GCAATG 1 cut(s) 1795
BsrFI RCCGGY 3 cut(s) 168, 272, 2187
BsrI ACTGG 6 cut(s) 450, 1178, 1264, 1678, 2267, 2510
BssAI RCCGGY 3 cut(s) 168, 272, 2187
BssECI CCNNGG 3 cut(s) 901, 1193, 1601
BssMI GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
BssNI GRCGYC 1 cut(s) 5
BssSI CACGAG 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 1601
Bst2BI CACGAG 1 cut(s) 102
Bst2UI CCWGG 2 cut(s) 778, 2258
Bst4CI ACNGT 9 cut(s) 394, 502, 673, 1157, 1304, 1340, 1514, 1779, 1872
Bst6I CTCTTC 4 cut(s) 798, 805, 1070, 1776
BstACI GRCGYC 1 cut(s) 5
BstBAI YACGTR 4 cut(s) 70, 213, 1100, 2232
BstC8I GCNNGC 3 cut(s) 772, 2159, 2430
BstDEI CTNAG 9 cut(s) 10, 861, 1292, 1493, 1536, 1716, 1746, 1752, 2112
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 7 cut(s) 859, 1189, 1216, 1405, 1469, 1918, 2006
BstHHI GCGC 1 cut(s) 377
BstKTI GATC 9 cut(s) 123, 1062, 1522, 1612, 1933, 1961, 2056, 2060, 2408
BstMAI GTCTC 8 cut(s) 33, 92, 409, 575, 824, 1288, 1755, 2516
BstMBI GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
BstMWI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1541, 1586, 1625, 2158
BstNI CCWGG 2 cut(s) 778, 2258
BstNSI RCATGY 3 cut(s) 346, 571, 2018
BstSCI CCNGG 5 cut(s) 776, 900, 1193, 1904, 2256
BstSFI CTRYAG 1 cut(s) 1590
BstSLI GKGCMC 1 cut(s) 336
BstSNI TACGTA 2 cut(s) 1100, 2232
BstV1I GCAGC 6 cut(s) 333, 990, 1543, 1681, 1751, 2004
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 3 cut(s) 1609, 1930, 1958
BstXI CCANNNNNNTGG 1 cut(s) 1685
BstYI RGATCY 3 cut(s) 1609, 1930, 1958
Bsu15I ATCGAT 2 cut(s) 1091, 2056
Bsu36I CCTNAGG 1 cut(s) 861
BsuI GTATCC 3 cut(s) 243, 1795, 2496
BsuRI GGCC 4 cut(s) 334, 704, 1081, 1318
BsuTUI ATCGAT 2 cut(s) 1091, 2056
BtrI CACGTC 2 cut(s) 725, 761
BtsCI GGATG 7 cut(s) 859, 1189, 1216, 1405, 1469, 1918, 2006
BtsIMutI CAGTG 7 cut(s) 216, 507, 1171, 1289, 1736, 1868, 2533
BveI ACCTGC 1 cut(s) 1873
Cac8I GCNNGC 3 cut(s) 772, 2159, 2430
CfoI GCGC 1 cut(s) 377
Cfr10I RCCGGY 3 cut(s) 168, 272, 2187
Cfr13I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1177, 1944
ClaI ATCGAT 2 cut(s) 1091, 2056
CsiI ACCWGGT 1 cut(s) 776
Csp6I GTAC 6 cut(s) 155, 214, 453, 2137, 2233, 2318
CspAI ACCGGT 3 cut(s) 168, 272, 2187
CviQI GTAC 6 cut(s) 155, 214, 453, 2137, 2233, 2318
DdeI CTNAG 9 cut(s) 10, 861, 1292, 1493, 1536, 1716, 1746, 1752, 2112
DpnI GATC 9 cut(s) 122, 1061, 1521, 1611, 1932, 1960, 2055, 2059, 2407
DpnII GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
DraI TTTAAA 2 cut(s) 697, 945
DrdI GACNNNNNNGTC 1 cut(s) 759
DseDI GACNNNNNNGTC 1 cut(s) 759
Eam1104I CTCTTC 4 cut(s) 798, 805, 1070, 1776
EarI CTCTTC 4 cut(s) 798, 805, 1070, 1776
EciI GGCGGA 1 cut(s) 262
Eco105I TACGTA 2 cut(s) 1100, 2232
Eco130I CCWWGG 1 cut(s) 1601
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 3 cut(s) 58, 336, 908
Eco31I GGTCTC 1 cut(s) 1288
Eco47I GGWCC 5 cut(s) 175, 505, 979, 1177, 1944
Eco57I CTGAAG 1 cut(s) 1650
Eco81I CCTNAGG 1 cut(s) 861
Eco88I CYCGRG 4 cut(s) 51, 633, 1083, 2477
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO109I RGGNCCY 3 cut(s) 332, 333, 1177
EcoRI GAATTC 4 cut(s) 433, 639, 1757, 2281
EcoRII CCWGG 2 cut(s) 776, 2256
EcoT14I CCWWGG 1 cut(s) 1601
EcoT22I ATGCAT 2 cut(s) 1249, 1546
EcoT38I GRGCYC 3 cut(s) 58, 336, 908
ErhI CCWWGG 1 cut(s) 1601
Esp3I CGTCTC 1 cut(s) 2516
FalI AAGNNNNNCTT 6 cut(s) 476, 508, 506, 538, 1933, 1965
FaqI GGGAC 2 cut(s) 1190, 1578
FauI CCCGC 1 cut(s) 1478
FauNDI CATATG 2 cut(s) 1243, 1546
FbaI TGATCA 1 cut(s) 1059
Fnu4HI GCNGC 7 cut(s) 43, 347, 1004, 1557, 1695, 1740, 1993
FokI GGATG 7 cut(s) 866, 1176, 1223, 1412, 1456, 1925, 1993
FriOI GRGCYC 3 cut(s) 58, 336, 908
Fsp4HI GCNGC 7 cut(s) 43, 347, 1004, 1557, 1695, 1740, 1993
FspBI CTAG 4 cut(s) 999, 1835, 1934, 2394
GlaI GCGC 1 cut(s) 376
GluI GCNGC 7 cut(s) 43, 347, 1004, 1557, 1695, 1740, 1993
GsaI CCCAGC 1 cut(s) 1289
HaeIII GGCC 4 cut(s) 334, 704, 1081, 1318
HapII CCGG 7 cut(s) 88, 169, 273, 901, 1195, 1905, 2188
HhaI GCGC 1 cut(s) 377
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 375
HinP1I GCGC 1 cut(s) 375
HincII GTYRAC 4 cut(s) 1282, 2254, 2362, 2512
HindII GTYRAC 4 cut(s) 1282, 2254, 2362, 2512
HinfI GANTC 9 cut(s) 203, 315, 646, 1054, 1088, 1883, 2077, 2087, 2378
HpaI GTTAAC 2 cut(s) 1282, 2362
HpaII CCGG 7 cut(s) 88, 169, 273, 901, 1195, 1905, 2188
HphI GGTGA 5 cut(s) 283, 641, 1385, 1673, 2085
HpyAV CCTTC 9 cut(s) 69, 251, 1178, 1329, 1716, 1903, 1935, 2410, 2416
HpyCH4III ACNGT 9 cut(s) 394, 502, 673, 1157, 1304, 1340, 1514, 1779, 1872
HpyF10VI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1541, 1586, 1625, 2158
HpyF3I CTNAG 9 cut(s) 10, 861, 1292, 1493, 1536, 1716, 1746, 1752, 2112
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 375
Ksp22I TGATCA 1 cut(s) 1059
KspAI GTTAAC 2 cut(s) 1282, 2362
Kzo9I GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
LmnI GCTCC 5 cut(s) 61, 1197, 1586, 2296, 2425
Lsp1109I GCAGC 6 cut(s) 333, 990, 1543, 1681, 1751, 2004
LweI GCATC 7 cut(s) 864, 1201, 1390, 1606, 1807, 2139, 2333
MabI ACCWGGT 1 cut(s) 776
MaeI CTAG 4 cut(s) 999, 1835, 1934, 2394
MaeIII GTNAC 5 cut(s) 289, 725, 924, 1340, 1661
MalI GATC 9 cut(s) 122, 1061, 1521, 1611, 1932, 1960, 2055, 2059, 2407
MbiI CCGCTC 1 cut(s) 1485
MboI GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
MfeI CAATTG 5 cut(s) 32, 295, 1119, 1501, 2331
MflI RGATCY 3 cut(s) 1609, 1930, 1958
MhlI GDGCHC 3 cut(s) 58, 336, 908
MlyI GAGTC 1 cut(s) 2096
MmeI TCCRAC 3 cut(s) 153, 292, 2493
Mph1103I ATGCAT 2 cut(s) 1249, 1546
MslI CAYNNNNRTG 1 cut(s) 1680
MspA1I CMGCKG 2 cut(s) 1694, 2348
MspI CCGG 7 cut(s) 88, 169, 273, 901, 1195, 1905, 2188
MspR9I CCNGG 5 cut(s) 778, 902, 1195, 1906, 2258
MunI CAATTG 5 cut(s) 32, 295, 1119, 1501, 2331
MvaI CCWGG 2 cut(s) 778, 2258
MwoI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1541, 1586, 1625, 2158
NciI CCSGG 3 cut(s) 902, 1195, 1906
NdeI CATATG 2 cut(s) 1243, 1546
NdeII GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
NlaIV GGNNCC 8 cut(s) 57, 177, 334, 775, 1037, 1178, 1690, 2298
NmuCI GTSAC 4 cut(s) 289, 725, 924, 1661
NsiI ATGCAT 2 cut(s) 1249, 1546
NspI RCATGY 3 cut(s) 346, 571, 2018
PaeR7I CTCGAG 2 cut(s) 1083, 2477
PceI AGGCCT 1 cut(s) 1081
PciI ACATGT 2 cut(s) 567, 2014
PfeI GAWTC 8 cut(s) 203, 315, 646, 1054, 1088, 1883, 2077, 2378
PflFI GACNNNGTC 1 cut(s) 1304
PfoI TCCNGGA 1 cut(s) 1904
PinAI ACCGGT 3 cut(s) 168, 272, 2187
PkrI GCNGC 7 cut(s) 44, 348, 1005, 1558, 1696, 1741, 1994
PleI GAGTC 1 cut(s) 2095
PpsI GAGTC 1 cut(s) 2095
Ppu21I YACGTR 4 cut(s) 70, 213, 1100, 2232
PpuMI RGGWCCY 1 cut(s) 1177
PscI ACATGT 2 cut(s) 567, 2014
PshBI ATTAAT 2 cut(s) 1131, 1824
Psp1406I AACGTT 1 cut(s) 2472
Psp5II RGGWCCY 1 cut(s) 1177
Psp6I CCWGG 2 cut(s) 776, 2256
PspFI CCCAGC 1 cut(s) 1285
PspGI CCWGG 2 cut(s) 776, 2256
PspN4I GGNNCC 8 cut(s) 57, 177, 334, 775, 1037, 1178, 1690, 2298
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1177, 1944
PspPPI RGGWCCY 1 cut(s) 1177
PsuI RGATCY 3 cut(s) 1609, 1930, 1958
PsyI GACNNNGTC 1 cut(s) 1304
RsaI GTAC 6 cut(s) 156, 215, 454, 2138, 2234, 2319
RsaNI GTAC 6 cut(s) 155, 214, 453, 2137, 2233, 2318
RseI CAYNNNNRTG 1 cut(s) 1680
SatI GCNGC 7 cut(s) 43, 347, 1004, 1557, 1695, 1740, 1993
Sau3AI GATC 9 cut(s) 120, 1059, 1519, 1609, 1930, 1958, 2053, 2057, 2405
Sau96I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1177, 1944
ScaI AGTACT 3 cut(s) 156, 454, 2319
SchI GAGTC 1 cut(s) 2096
ScrFI CCNGG 5 cut(s) 778, 902, 1195, 1906, 2258
SduI GDGCHC 3 cut(s) 58, 336, 908
SexAI ACCWGGT 1 cut(s) 776
SfaNI GCATC 7 cut(s) 864, 1201, 1390, 1606, 1807, 2139, 2333
SfcI CTRYAG 1 cut(s) 1590
Sfr274I CTCGAG 2 cut(s) 1083, 2477
SinI GGWCC 5 cut(s) 175, 505, 979, 1177, 1944
SlaI CTCGAG 2 cut(s) 1083, 2477
SmiMI CAYNNNNRTG 1 cut(s) 1680
SmlI CTYRAG 2 cut(s) 1083, 2477
SmoI CTYRAG 2 cut(s) 1083, 2477
SnaBI TACGTA 2 cut(s) 1100, 2232
SpeI ACTAGT 1 cut(s) 2393
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 6 cut(s) 42, 247, 425, 1485, 1692, 2348
SspI AATATT 2 cut(s) 966, 1441
SspMI CTAG 4 cut(s) 999, 1835, 1934, 2394
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 5 cut(s) 776, 900, 1193, 1904, 2256
StyI CCWWGG 1 cut(s) 1601
TaaI ACNGT 9 cut(s) 394, 502, 673, 1157, 1304, 1340, 1514, 1779, 1872
TaqI TCGA 6 cut(s) 1084, 1091, 1335, 2056, 2147, 2478
TatI WGTACW 3 cut(s) 154, 452, 2317
TauI GCSGC 1 cut(s) 45
TfiI GAWTC 8 cut(s) 203, 315, 646, 1054, 1088, 1883, 2077, 2378
TscAI CASTG 6 cut(s) 223, 507, 1178, 1296, 1736, 1875
TseFI GTSAC 4 cut(s) 289, 725, 924, 1661
TseI GCWGC 6 cut(s) 346, 1003, 1556, 1694, 1739, 1992
Tsp45I GTSAC 4 cut(s) 289, 725, 924, 1661
TspGWI ACGGA 2 cut(s) 1223, 2379
TspRI CASTG 6 cut(s) 223, 507, 1178, 1296, 1736, 1875
Tth111I GACNNNGTC 1 cut(s) 1304
VpaK11BI GGWCC 5 cut(s) 175, 505, 979, 1177, 1944
VspI ATTAAT 2 cut(s) 1131, 1824
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 9 cut(s) 83, 323, 433, 639, 1163, 1757, 1894, 2030, 2281
XbaI TCTAGA 1 cut(s) 1933
XceI RCATGY 3 cut(s) 346, 571, 2018
XhoI CTCGAG 2 cut(s) 1083, 2477
XspI CTAG 4 cut(s) 999, 1835, 1934, 2394
ZrmI AGTACT 3 cut(s) 156, 454, 2319
Zsp2I ATGCAT 2 cut(s) 1249, 1546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.