MD15G1223800.v1.1

phosphoinositide phosphatase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007042
Physical Location & Seq
Reverse (-)
18180491 .. 18188633
8143 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1223800.v1.1.491

Sequence Viewer

Length: 2541 bp
ATGGCGTCCTCAGAGAATGAGCCTCTGTCCCAATTGTGTGGCGACGAAACTCGGCCTCCTCCTCAACACGTAGATATGCAGAAATTCCGGCTATACGGGACTCGATCGAACTTTTACATGATTGGGAGAGATAAGAGTAGAACATATTGGAGAGTGCTAAAGATTGACCGGTTGGACCCTTGTGAGCTTAACATTCGTGAAGATTCTACTACGTACACCGAAAGAGAATGTTCTGACCTGTTGAGGCGGATACATGAAGGGAACAAGGCCACCGGTGGATTGAAGTTTGTTACCACTTGTTACGGAATTGTTGGATTTGTAAAATTTCTGGGGCCCTATTACATGCTGCTTATCACGAAAAGAAGACAAATAGGTGCCATATGTGGGCATACGGTCTATGCAATTTCCAAGAGTGAAATGATACCACTTCCAAATTCTTCTGTCCTATCCAGTACCACTAATTCTAAAAATGAAAACAGATATAAGAAGCTCTTAAGCACAGTGGACCTTACAAAGGACTTCTTTTTCAGCTACTCATACCATGTTATGCGTAGTCTGCAAAAGAACATGTGTAAGAATGAGACAGGTCATGTCCTTTATGAAACCATGTTTGTCTGGAATGAGTTCTTAACCCGGGGAATTCGGAATCACCTCCAAAATACCGTTTGGACAGTTGCATTGGTTTATGGCTTCTTTAAACAGGCCACTTTCTCTATATCTGGACGTGACTTCAAGCTGACCCTTATTGCAAGACGTTCACGTCATTATGCAGGCACCAGGTATCTAAAACGAGGTGTTAATGAGAAGGGAAGAGTAGCTAATGATGTTGAGACAGAACAGATTGTCTGTGAGGATGTTCCTGAGGGGTTTCCTATGCAAATAAGTTCTGTTGTGCAGAACCGGGGCTCAATCCCTCTATTTTGGTCACAGGAAACCTCACGTTTAAATATCAAACCAGATATAATATTGTCAAAGAAGGACCAAAACTATGAAGCCACTAGGCTTCACTTTGAAAATCTTGTCAAGAGATATGGGAACCCCGTAATTATTTTGAATCTGATCAAGACACAAGAGAAGAGGCCTCGAGAGTCCATACTGCGTACAGAGTTTGCTAATGCAATTGATTTCATTAATAAAGACCTATCTGATGAGAACCGTCTGAGATTCCTTCACTGGGATCTCCACAAGCATTCCCGGAGCAAAGCTATAAATGCTTTACTACTCTTGGGCAAGGTGGCTACATATGCCTTGACACTAACTGGGTTCTTCTATTGTCAAGTTAACCCAACACTGCGACCTGACAGTGTCATTAAGTGGCCTTCCTCTGGGCATTTTGACAGTGGTAACTTGTCTGTTGAAATACATAATGCTAATGGTGATGAGGAAGCTAATGGTAAAGAGGATGCCGATAACATAGAGTGTAAACCAAGTGGAGGCAGAAATATTGCAAATGGTGATCATTCTGTCAAGCCATCCATGTTCCAGAGTGGGGTGCTTAGAACCAACTGCATAGACTGTCTGGATCGCACAAATGTTGCTCAGTATGCATATGGGTTGGCTGCATTGGGACACCAGCTTCATGCTTTGGGAGCTATAGACAATCCAAGGATAGACCTTGATGCTCCTTTGGCTGATGACTTGATGGGATTTTACGAAAGAATGGGTGACACACTTGCACACCAGTACGGTGGTTCCGCTGCTCATAACAAGATATTTTCTGAGAGAAGGGGTCAGTGGAGAGCAGCAACTCAGTCTCAGGAGTTCTTCAGAACTCTTCAACGGTATTACAGCAATGCTTATATGGATGCAGAGAAGCAAGATGCAATTAATGTATTTCTTGGGCATTTTCAACCACAAGAAGGTAAACCTGCACTGTGGGAATTGGATTCTGACCAACATTATTACTCTGGGAGGGATGAAACAAAAGATAAGGATCTAGATGGAAGGTCCATTTTCAAAAGATGTTATTCAGATGGCAACATTCTTCGAGAAAGCAGCTCACCTATTTCATCCACACATGTTAAGCAGAAATTTACAAACCCTTGTATGCCTGATCGATCACAACAAGAAAGCAAGATTCTTTCGGAGTCATCACCTGATATATCAACTTCTGAGAGTGATATTGCATTTTCAAGGTACACTCCTTCAATGCCTCGCAGGCAACTGTTTGGAGAGTTTCAAAGATTCCGGTGTGAAAGTGATCATATTTACTATTCTGAACACGGAGATTCGTACAACTTCTCAAACTTTGTTGACCTAGACTGGCTATCTTCATCTGCAAATTCTTGTGAGGAGGAGCCATTTGAGAGGTCGTCAGTACTTACAAACTCTCCAATTGAGGGGCAATCATCGGAGAATATTGTTAATGGAATAATGGGGGATCCTACTCCGTCTACAAGTGAATATGGACCAAGCTTGAAGGGGAAGGAGCAGACAGGAACGGGGTTGTCCTTTAATAACGGGGAGAGTTCCAATGTTCTCGAGGAGTACCCCGATAGCTTTGTGAACTGGGTCAACTTCGGAGAGACGCTATGCCACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004438 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005811 GO:0005911 GO:0006629 GO:0006644 GO:0006650 GO:0006793 GO:0006796 GO:0006996 GO:0007033 GO:0007272 GO:0007275 GO:0007399 GO:0007610 GO:0007626 GO:0008150 GO:0008152 GO:0008366 GO:0009506 GO:0009653 GO:0009987 GO:0010008 GO:0010720 GO:0010927 GO:0010975 GO:0010976 GO:0012505 GO:0016020 GO:0016043 GO:0016311 GO:0016787 GO:0016788 GO:0016791 GO:0019637 GO:0022008 GO:0022607 GO:0030054 GO:0030154 GO:0030182 GO:0031090 GO:0031344 GO:0031346 GO:0031410 GO:0031641 GO:0031642 GO:0031644 GO:0031645 GO:0031982 GO:0032288 GO:0032501 GO:0032502 GO:0032989 GO:0034593 GO:0034596 GO:0042552 GO:0042578 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043473 GO:0043812 GO:0044057 GO:0044085 GO:0044237 GO:0044238 GO:0044255 GO:0044422 GO:0044424 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045595 GO:0045597 GO:0045664 GO:0045666 GO:0046486 GO:0046488 GO:0048468 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0048646 GO:0048666 GO:0048699 GO:0048731 GO:0048856 GO:0048869 GO:0050767 GO:0050769 GO:0050789 GO:0050793 GO:0050794 GO:0051093 GO:0051094 GO:0051128 GO:0051130 GO:0051239 GO:0051240 GO:0051241 GO:0051960 GO:0051961 GO:0051962 GO:0052744 GO:0052866 GO:0055044 GO:0060284 GO:0065007 GO:0071704 GO:0071840 GO:0097708 GO:0098588 GO:0098805 GO:0120035 GO:2000026
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Syja_N

Protein Analysis

847

Amino Acids

96.39

Weight (kDa)

6.41

Isoelectric Point (pI)

50.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syja_N PF02383 101 - 399 4.8e-85 SacI homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 759
Acc36I ACCTGC 1 cut(s) 1876
AccB1I GGYRCC 2 cut(s) 374, 773
AccI GTMKAC 1 cut(s) 2393
AciI CCGC 2 cut(s) 247, 1695
AclWI GGATC 5 cut(s) 1185, 1530, 1941, 2375, 2388
AcsI RAATTY 6 cut(s) 83, 323, 433, 639, 2030, 2281
AcuI CTGAAG 1 cut(s) 1750
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 8 cut(s) 215, 454, 1102, 1685, 2138, 2234, 2319, 2489
AfiI CCNNNNNNNGG 8 cut(s) 384, 514, 1174, 1325, 1433, 1489, 1859, 2339
AflII CTTAAG 1 cut(s) 493
AflIII ACRYGT 3 cut(s) 67, 567, 2017
AgeI ACCGGT 2 cut(s) 168, 272
AjiI CACGTC 2 cut(s) 725, 761
AjnI CCWGG 1 cut(s) 776
Alw26I GTCTC 4 cut(s) 575, 824, 1758, 2519
AlwI GGATC 5 cut(s) 1185, 1530, 1941, 2375, 2388
Ama87I CYCGRG 3 cut(s) 633, 1083, 2480
AoxI GGCC 6 cut(s) 53, 267, 332, 702, 1079, 1316
ApaI GGGCCC 1 cut(s) 336
ApeKI GCWGC 5 cut(s) 346, 1559, 1697, 1742, 1995
ApoI RAATTY 6 cut(s) 83, 323, 433, 639, 2030, 2281
AseI ATTAAT 2 cut(s) 1131, 1827
AsiGI ACCGGT 2 cut(s) 168, 272
Asp700I GAANNNNTTC 1 cut(s) 623
AspS9I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1947, 2408
AsuC2I CCSGG 4 cut(s) 634, 635, 902, 1195
AsuHPI GGTGA 6 cut(s) 641, 1388, 1466, 1676, 1992, 2085
AvaI CYCGRG 3 cut(s) 633, 1083, 2480
AvaII GGWCC 5 cut(s) 175, 505, 979, 1947, 2408
AxyI CCTNAGG 1 cut(s) 861
BaeGI GKGCMC 1 cut(s) 336
BamHI GGATCC 1 cut(s) 2380
BanI GGYRCC 2 cut(s) 374, 773
BanII GRGCYC 2 cut(s) 336, 908
BbsI GAAGAC 1 cut(s) 370
BbvI GCAGC 5 cut(s) 333, 1546, 1684, 1754, 2007
BccI CCATC 4 cut(s) 1480, 1636, 1934, 1967
BciT130I CCWGG 1 cut(s) 778
BciVI GTATCC 1 cut(s) 243
BclI TGATCA 3 cut(s) 1059, 1456, 2200
BcnI CCSGG 4 cut(s) 634, 635, 902, 1195
BcoDI GTCTC 4 cut(s) 575, 824, 1758, 2519
BfaI CTAG 3 cut(s) 999, 1937, 2258
BfmI CTRYAG 1 cut(s) 1593
BfrI CTTAAG 1 cut(s) 493
BfuAI ACCTGC 1 cut(s) 1876
BfuI GTATCC 1 cut(s) 243
BglI GCCNNNNNGGC 1 cut(s) 2158
BisI GCNGC 5 cut(s) 347, 1560, 1698, 1743, 1996
BlsI GCNGC 5 cut(s) 348, 1561, 1699, 1744, 1997
BmcAI AGTACT 1 cut(s) 2319
Bme1390I CCNGG 5 cut(s) 634, 635, 778, 902, 1195
Bme18I GGWCC 5 cut(s) 175, 505, 979, 1947, 2408
BmeT110I CYCGRG 3 cut(s) 633, 1083, 2480
BmgBI CACGTC 2 cut(s) 725, 761
BmgT120I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1947, 2408
BmiI GGNNCC 9 cut(s) 177, 333, 334, 376, 775, 1037, 1693, 2298, 2382
BmrFI CCNGG 5 cut(s) 634, 635, 778, 902, 1195
BmrI ACTGGG 3 cut(s) 1183, 1269, 2518
BmsI GCATC 4 cut(s) 1393, 1609, 1795, 1810
BmuI ACTGGG 3 cut(s) 1183, 1269, 2518
BpiI GAAGAC 1 cut(s) 370
BpuMI CCSGG 4 cut(s) 634, 635, 902, 1195
Bsa29I ATCGAT 1 cut(s) 2056
BsaAI YACGTR 2 cut(s) 70, 213
BsaBI GATNNNNATC 1 cut(s) 1932
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 4 cut(s) 633, 634, 901, 1604
BsaWI WCCGGW 3 cut(s) 168, 272, 2187
BsaXI ACNNNNNCTCC 4 cut(s) 40, 70, 2314, 2344
Bsc4I CCNNNNNNNGG 8 cut(s) 384, 514, 1174, 1325, 1433, 1489, 1859, 2339
Bse118I RCCGGY 2 cut(s) 168, 272
Bse1I ACTGG 6 cut(s) 450, 1178, 1264, 1681, 2267, 2513
Bse21I CCTNAGG 1 cut(s) 861
Bse3DI GCAATG 1 cut(s) 1798
Bse8I GATNNNNATC 1 cut(s) 1932
BseBI CCWGG 1 cut(s) 778
BseCI ATCGAT 1 cut(s) 2056
BseDI CCNNGG 4 cut(s) 633, 634, 901, 1604
BseGI GGATG 6 cut(s) 859, 1408, 1472, 1810, 1921, 2009
BseJI GATNNNNATC 1 cut(s) 1932
BseLI CCNNNNNNNGG 8 cut(s) 384, 514, 1174, 1325, 1433, 1489, 1859, 2339
BseMI GCAATG 1 cut(s) 1798
BseMII CTCAG 8 cut(s) 24, 852, 1151, 1553, 1710, 1763, 1769, 2103
BseNI ACTGG 6 cut(s) 450, 1178, 1264, 1681, 2267, 2513
BseRI GAGGAG 5 cut(s) 48, 51, 2306, 2309, 2498
BseSI GKGCMC 1 cut(s) 336
BseXI GCAGC 5 cut(s) 333, 1546, 1684, 1754, 2007
BsgI GTGCAG 2 cut(s) 914, 1854
Bsh1285I CGRYCG 1 cut(s) 107
BshFI GGCC 6 cut(s) 55, 269, 334, 704, 1081, 1318
BshNI GGYRCC 2 cut(s) 374, 773
BshTI ACCGGT 2 cut(s) 168, 272
BshVI ATCGAT 1 cut(s) 2056
BsiEI CGRYCG 1 cut(s) 107
BsiHKCI CYCGRG 3 cut(s) 633, 1083, 2480
BsiSI CCGG 7 cut(s) 88, 169, 273, 634, 901, 1195, 2188
BslFI GGGAC 3 cut(s) 13, 112, 1581
BslI CCNNNNNNNGG 8 cut(s) 384, 514, 1174, 1325, 1433, 1489, 1859, 2339
BsmAI GTCTC 4 cut(s) 575, 824, 1758, 2519
BsmBI CGTCTC 1 cut(s) 2519
BsmFI GGGAC 3 cut(s) 13, 112, 1581
BsmI GAATGC 1 cut(s) 1189
BsnI GGCC 6 cut(s) 55, 269, 334, 704, 1081, 1318
BsoBI CYCGRG 3 cut(s) 633, 1083, 2480
Bsp120I GGGCCC 1 cut(s) 332
Bsp1286I GDGCHC 2 cut(s) 336, 908
BspACI CCGC 2 cut(s) 247, 1695
BspANI GGCC 6 cut(s) 55, 269, 334, 704, 1081, 1318
BspCNI CTCAG 8 cut(s) 23, 853, 1152, 1552, 1711, 1762, 1768, 2104
BspDI ATCGAT 1 cut(s) 2056
BspLI GGNNCC 9 cut(s) 177, 333, 334, 376, 775, 1037, 1693, 2298, 2382
BspMI ACCTGC 1 cut(s) 1876
BspPI GGATC 5 cut(s) 1185, 1530, 1941, 2375, 2388
BspT107I GGYRCC 2 cut(s) 374, 773
BspTI CTTAAG 1 cut(s) 493
BsrDI GCAATG 1 cut(s) 1798
BsrFI RCCGGY 2 cut(s) 168, 272
BsrI ACTGG 6 cut(s) 450, 1178, 1264, 1681, 2267, 2513
BssAI RCCGGY 2 cut(s) 168, 272
BssECI CCNNGG 4 cut(s) 633, 634, 901, 1604
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 1604
Bst2UI CCWGG 1 cut(s) 778
Bst6I CTCTTC 3 cut(s) 805, 1070, 1779
BstACI GRCGYC 1 cut(s) 5
BstAFI CTTAAG 1 cut(s) 493
BstBAI YACGTR 2 cut(s) 70, 213
BstC8I GCNNGC 2 cut(s) 772, 2159
BstDEI CTNAG 9 cut(s) 10, 861, 1160, 1496, 1539, 1719, 1749, 1755, 2112
BstENI CCTNNNNNAGG 1 cut(s) 512
BstF5I GGATG 6 cut(s) 859, 1408, 1472, 1810, 1921, 2009
BstMAI GTCTC 4 cut(s) 575, 824, 1758, 2519
BstMCI CGRYCG 1 cut(s) 107
BstMWI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1544, 1589, 1628, 2158
BstNI CCWGG 1 cut(s) 778
BstNSI RCATGY 3 cut(s) 346, 571, 2021
BstSCI CCNGG 5 cut(s) 632, 633, 776, 900, 1193
BstSFI CTRYAG 1 cut(s) 1593
BstSLI GKGCMC 1 cut(s) 336
BstSNI TACGTA 1 cut(s) 213
BstV1I GCAGC 5 cut(s) 333, 1546, 1684, 1754, 2007
BstV2I GAAGAC 1 cut(s) 370
BstX2I RGATCY 3 cut(s) 1177, 1933, 2380
BstXI CCANNNNNNTGG 2 cut(s) 38, 1688
BstYI RGATCY 3 cut(s) 1177, 1933, 2380
Bsu15I ATCGAT 1 cut(s) 2056
Bsu36I CCTNAGG 1 cut(s) 861
BsuI GTATCC 1 cut(s) 243
BsuRI GGCC 6 cut(s) 55, 269, 334, 704, 1081, 1318
BsuTUI ATCGAT 1 cut(s) 2056
BtrI CACGTC 2 cut(s) 725, 761
BtsCI GGATG 6 cut(s) 859, 1408, 1472, 1810, 1921, 2009
BtsI GCAGTG 1 cut(s) 1289
BtsIMutI CAGTG 8 cut(s) 507, 1171, 1289, 1309, 1345, 1739, 1871, 2536
BveI ACCTGC 1 cut(s) 1876
Cac8I GCNNGC 2 cut(s) 772, 2159
Cfr10I RCCGGY 2 cut(s) 168, 272
Cfr13I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1947, 2408
Cfr9I CCCGGG 1 cut(s) 633
ClaI ATCGAT 1 cut(s) 2056
CseI GACGC 1 cut(s) 2536
CsiI ACCWGGT 1 cut(s) 776
Csp6I GTAC 8 cut(s) 214, 453, 1101, 1684, 2137, 2233, 2318, 2488
CspAI ACCGGT 2 cut(s) 168, 272
CviQI GTAC 8 cut(s) 214, 453, 1101, 1684, 2137, 2233, 2318, 2488
DdeI CTNAG 9 cut(s) 10, 861, 1160, 1496, 1539, 1719, 1749, 1755, 2112
DraI TTTAAA 2 cut(s) 697, 945
DrdI GACNNNNNNGTC 1 cut(s) 759
DseDI GACNNNNNNGTC 1 cut(s) 759
Eam1104I CTCTTC 3 cut(s) 805, 1070, 1779
EarI CTCTTC 3 cut(s) 805, 1070, 1779
EciI GGCGGA 1 cut(s) 262
Eco105I TACGTA 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 1604
Eco147I AGGCCT 1 cut(s) 1081
Eco24I GRGCYC 2 cut(s) 336, 908
Eco47I GGWCC 5 cut(s) 175, 505, 979, 1947, 2408
Eco57I CTGAAG 1 cut(s) 1750
Eco81I CCTNAGG 1 cut(s) 861
Eco88I CYCGRG 3 cut(s) 633, 1083, 2480
EcoNI CCTNNNNNAGG 1 cut(s) 512
EcoO109I RGGNCCY 2 cut(s) 332, 333
EcoRI GAATTC 1 cut(s) 639
EcoRII CCWGG 1 cut(s) 776
EcoT14I CCWWGG 1 cut(s) 1604
EcoT22I ATGCAT 1 cut(s) 1549
EcoT38I GRGCYC 2 cut(s) 336, 908
ErhI CCWWGG 1 cut(s) 1604
Esp3I CGTCTC 1 cut(s) 2519
FalI AAGNNNNNCTT 4 cut(s) 476, 508, 506, 538
FaqI GGGAC 3 cut(s) 13, 112, 1581
FauNDI CATATG 3 cut(s) 380, 1243, 1549
FbaI TGATCA 3 cut(s) 1059, 1456, 2200
FblI GTMKAC 1 cut(s) 2393
Fnu4HI GCNGC 5 cut(s) 347, 1560, 1698, 1743, 1996
FokI GGATG 6 cut(s) 866, 1415, 1459, 1817, 1928, 1996
FriOI GRGCYC 2 cut(s) 336, 908
Fsp4HI GCNGC 5 cut(s) 347, 1560, 1698, 1743, 1996
FspBI CTAG 3 cut(s) 999, 1937, 2258
GluI GCNGC 5 cut(s) 347, 1560, 1698, 1743, 1996
HaeIII GGCC 6 cut(s) 55, 269, 334, 704, 1081, 1318
HapII CCGG 7 cut(s) 88, 169, 273, 634, 901, 1195, 2188
HgaI GACGC 1 cut(s) 2536
Hin1I GRCGYC 1 cut(s) 5
HincII GTYRAC 3 cut(s) 1282, 2254, 2515
HindII GTYRAC 3 cut(s) 1282, 2254, 2515
HindIII AAGCTT 1 cut(s) 2413
HpaI GTTAAC 1 cut(s) 1282
HpaII CCGG 7 cut(s) 88, 169, 273, 634, 901, 1195, 2188
HphI GGTGA 6 cut(s) 641, 1388, 1466, 1676, 1992, 2085
Hpy99I CGWCG 1 cut(s) 47
HpyCH4IV ACGT 6 cut(s) 69, 212, 724, 754, 760, 940
HpyF10VI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1544, 1589, 1628, 2158
HpyF3I CTNAG 9 cut(s) 10, 861, 1160, 1496, 1539, 1719, 1749, 1755, 2112
HpySE526I ACGT 6 cut(s) 69, 212, 724, 754, 760, 940
Hsp92I GRCGYC 1 cut(s) 5
Ksp22I TGATCA 3 cut(s) 1059, 1456, 2200
KspAI GTTAAC 1 cut(s) 1282
LmnI GCTCC 5 cut(s) 1197, 1589, 1627, 2296, 2428
Lsp1109I GCAGC 5 cut(s) 333, 1546, 1684, 1754, 2007
LweI GCATC 4 cut(s) 1393, 1609, 1795, 1810
MabI ACCWGGT 1 cut(s) 776
MaeI CTAG 3 cut(s) 999, 1937, 2258
MaeII ACGT 6 cut(s) 69, 212, 724, 754, 760, 940
MaeIII GTNAC 6 cut(s) 289, 299, 725, 924, 1343, 1664
MfeI CAATTG 3 cut(s) 32, 1119, 2334
MflI RGATCY 3 cut(s) 1177, 1933, 2380
MhlI GDGCHC 2 cut(s) 336, 908
MlyI GAGTC 3 cut(s) 94, 1097, 2096
MmeI TCCRAC 2 cut(s) 153, 292
Mph1103I ATGCAT 1 cut(s) 1549
MroXI GAANNNNTTC 1 cut(s) 623
MspA1I CMGCKG 1 cut(s) 1697
MspCI CTTAAG 1 cut(s) 493
MspI CCGG 7 cut(s) 88, 169, 273, 634, 901, 1195, 2188
MspR9I CCNGG 5 cut(s) 634, 635, 778, 902, 1195
MunI CAATTG 3 cut(s) 32, 1119, 2334
Mva1269I GAATGC 1 cut(s) 1189
MvaI CCWGG 1 cut(s) 778
MwoI GCNNNNNNNGC 7 cut(s) 556, 1211, 1244, 1544, 1589, 1628, 2158
NciI CCSGG 4 cut(s) 634, 635, 902, 1195
NdeI CATATG 3 cut(s) 380, 1243, 1549
NlaIV GGNNCC 9 cut(s) 177, 333, 334, 376, 775, 1037, 1693, 2298, 2382
NmeAIII GCCGAG 1 cut(s) 31
NmuCI GTSAC 3 cut(s) 725, 924, 1664
NsiI ATGCAT 1 cut(s) 1549
NspI RCATGY 3 cut(s) 346, 571, 2021
PaeR7I CTCGAG 2 cut(s) 1083, 2480
PceI AGGCCT 1 cut(s) 1081
PciI ACATGT 2 cut(s) 567, 2017
PctI GAATGC 1 cut(s) 1189
PdmI GAANNNNTTC 1 cut(s) 623
PfeI GAWTC 8 cut(s) 203, 646, 1054, 1164, 1886, 2077, 2184, 2228
PflFI GACNNNGTC 1 cut(s) 1304
PfoI TCCNGGA 1 cut(s) 1193
PinAI ACCGGT 2 cut(s) 168, 272
PkrI GCNGC 5 cut(s) 348, 1561, 1699, 1744, 1997
Ple19I CGATCG 1 cut(s) 107
PleI GAGTC 3 cut(s) 94, 1096, 2095
PpsI GAGTC 3 cut(s) 94, 1096, 2095
Ppu21I YACGTR 2 cut(s) 70, 213
PscI ACATGT 2 cut(s) 567, 2017
PshBI ATTAAT 2 cut(s) 1131, 1827
Psp6I CCWGG 1 cut(s) 776
PspGI CCWGG 1 cut(s) 776
PspN4I GGNNCC 9 cut(s) 177, 333, 334, 376, 775, 1037, 1693, 2298, 2382
PspOMI GGGCCC 1 cut(s) 332
PspPI GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1947, 2408
PsuI RGATCY 3 cut(s) 1177, 1933, 2380
PsyI GACNNNGTC 1 cut(s) 1304
PvuI CGATCG 1 cut(s) 107
RsaI GTAC 8 cut(s) 215, 454, 1102, 1685, 2138, 2234, 2319, 2489
RsaNI GTAC 8 cut(s) 214, 453, 1101, 1684, 2137, 2233, 2318, 2488
SatI GCNGC 5 cut(s) 347, 1560, 1698, 1743, 1996
Sau96I GGNCC 7 cut(s) 175, 332, 333, 505, 979, 1947, 2408
ScaI AGTACT 1 cut(s) 2319
SchI GAGTC 3 cut(s) 94, 1097, 2096
ScrFI CCNGG 5 cut(s) 634, 635, 778, 902, 1195
SduI GDGCHC 2 cut(s) 336, 908
SexAI ACCWGGT 1 cut(s) 776
SfaNI GCATC 4 cut(s) 1393, 1609, 1795, 1810
SfcI CTRYAG 1 cut(s) 1593
Sfr274I CTCGAG 2 cut(s) 1083, 2480
SgrAI CRCCGGYG 1 cut(s) 272
SinI GGWCC 5 cut(s) 175, 505, 979, 1947, 2408
SlaI CTCGAG 2 cut(s) 1083, 2480
SmaI CCCGGG 1 cut(s) 635
SmlI CTYRAG 3 cut(s) 493, 1083, 2480
SmoI CTYRAG 3 cut(s) 493, 1083, 2480
SnaBI TACGTA 1 cut(s) 213
SseBI AGGCCT 1 cut(s) 1081
SsiI CCGC 2 cut(s) 247, 1695
SspI AATATT 3 cut(s) 966, 1444, 2359
SspMI CTAG 3 cut(s) 999, 1937, 2258
StuI AGGCCT 1 cut(s) 1081
StyD4I CCNGG 5 cut(s) 632, 633, 776, 900, 1193
StyI CCWWGG 1 cut(s) 1604
TaiI ACGT 6 cut(s) 72, 215, 727, 757, 763, 943
TaqI TCGA 6 cut(s) 103, 107, 1084, 1987, 2056, 2481
TatI WGTACW 1 cut(s) 2317
TfiI GAWTC 8 cut(s) 203, 646, 1054, 1164, 1886, 2077, 2184, 2228
TscAI CASTG 7 cut(s) 507, 1178, 1296, 1309, 1345, 1739, 1878
TseFI GTSAC 3 cut(s) 725, 924, 1664
TseI GCWGC 5 cut(s) 346, 1559, 1697, 1742, 1995
Tsp45I GTSAC 3 cut(s) 725, 924, 1664
TspDTI ATGAA 9 cut(s) 270, 486, 615, 1005, 1117, 1568, 1932, 1998, 2262
TspGWI ACGGA 3 cut(s) 318, 2238, 2379
TspMI CCCGGG 1 cut(s) 633
TspRI CASTG 7 cut(s) 507, 1178, 1296, 1309, 1345, 1739, 1878
Tth111I GACNNNGTC 1 cut(s) 1304
Vha464I CTTAAG 1 cut(s) 493
VpaK11BI GGWCC 5 cut(s) 175, 505, 979, 1947, 2408
VspI ATTAAT 2 cut(s) 1131, 1827
XagI CCTNNNNNAGG 1 cut(s) 512
XapI RAATTY 6 cut(s) 83, 323, 433, 639, 2030, 2281
XbaI TCTAGA 1 cut(s) 1936
XceI RCATGY 3 cut(s) 346, 571, 2021
XhoI CTCGAG 2 cut(s) 1083, 2480
XmaI CCCGGG 1 cut(s) 633
XmiI GTMKAC 1 cut(s) 2393
XmnI GAANNNNTTC 1 cut(s) 623
XspI CTAG 3 cut(s) 999, 1937, 2258
ZrmI AGTACT 1 cut(s) 2319
Zsp2I ATGCAT 1 cut(s) 1549
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.