FvH4_1g11630

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb1
OG0006904
Physical Location & Seq
Reverse (-)
6354364 .. 6358144
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_1g11630.t2

Sequence Viewer

Length: 1647 bp
ATGGGGGAGGGGAAGGGGGAAGGTCGATCCTTGGAACAAACACCCACATGGACTGTGGCTACAGTGATCACTGCCATGGTTTTAATTGTGTTGATCTTTCACAGCTCAATGAAACATTTTGGAAAGTGGTTGGATAGGACTAGAAAGAAGTCTATACTTGCTGCTTTGGAGAAGATCCAAGAAGCAGAGCTCATGCTTTTTGGGCTTATGTCATTGTTGATGGGTCACTGGATTGTTTTTGTGGCCAAGATTTGTGTTAAAACTTCTTCATCGGCCTTCAGCAGCAAATTCTTTCCTTGTGCTTTGGATGAAGATATCAGTTCGAGTCGTGAGTATCTAGACAAGTCTGTTTCTAGAGAGGAAGTAGAACCAAAGTTTCATCAATATTGTCCTGAGGGTGAGGAGTCCTTTGCTTCTTATGAAAGTCTTGAACAGCTTCATCGGTTAATATTCGTGCTTGGCATGACTCATGTTGTTTACAGTTTCTTTGCCATTGTCCTGGCCATGATTAAGATATATAGCTGGAGAACATGGGAAAATGAAGCTAAAGCCAAGGCATTTCAGAGCTTAGAAGGTAACCCAGAAACTAGACAAATCAGAAGATTGTCAACATTCATAGTTCACCATGTGTCCCATCCATGGAGCCAGCACAAGTATCTTGTTTGGCTGCTTTGTTTCAGCCGCCAGTTCTGGAGTTCCATAAACCGACCTTCTTATATGGCTTTGCGATTTGGATTCATCACTACTCATAAGCTTCCATTGATATATGATTTCCATAATTATATGTTACGGAGCATGGAGGAAGAATTTCGTGATATTGTTGGTATCAGTATACCTCTCTGGATATATGCCATATGTTGCATTATACTAGATTTTCATGGAAGTAACTTGTACTTTTGGCTTTCCTTCCTTCCAGCTATTTTGATCTTGCTTATGGGTACAAAACTGCATCGGATAGTGGTAAAATTGGCTGTGGAAATCATGGAAGAAAGTCCATATATGGAAAATCATCAGTTCAAACTAAGGGATGAGCTCTTTTGGTTCAAGAGGCCACGGCTTCTTTTACGGATAATACAACTGATATCCTTCCAGAATGCATTCGAGATGGCTACGTTCACCTGGTCTCTGTGGGAAATTAAACATCCTTCATGTTTCATGGACAATCAAACTTTCCTTGTGATCCGCTTGACATTTGGGATACTCTCCCAGTTCTGGTGTAGCTTCAGTACATTCCCACTCTATGTTATCATCACACAAATGGGTTCAAGGTTCAAGAAAGCTGTAGTTTCCGAAAATGTTAGACGATCACTATATGGATGGAAAAGGAGAGTGAAGGCTAGACAAAGTCCTTCATCTAGTACGGTTGGACTCCTCAGTTCACCATCTTCATTCACATCAGATTCTAGCAAAGTTTATGGTTCTGCCTCAAGAAGCATGGAAGGGAGCACAGTCCCCTCCTGCAGGGTCCAAAAAACATCTTTCCCACATGGTAACTCCAGTGTGCAGCTTGGTCAAGTTAGTGAGGGCTACGAGAAGTTAGAGTCTGAGCTCGGATCGCCAACGCTCGCTGCCCTGATGAGTGACTATGACCTTTATGAGGAAGATGAAGAAGATGATTACCATGAGGATGACAACAGTAGAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

549

Amino Acids

63.5

Weight (kDa)

7.29

Isoelectric Point (pI)

50.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 460 9e-158 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 832
AciI CCGC 2 cut(s) 682, 1183
AclWI GGATC 4 cut(s) 21, 169, 1174, 1561
AcoI YGGCCR 2 cut(s) 243, 501
AcsI RAATTY 2 cut(s) 287, 806
AcuI CTGAAG 2 cut(s) 262, 1207
AdeI CACNNNGTG 1 cut(s) 628
AfaI GTAC 4 cut(s) 893, 940, 1228, 1360
AfiI CCNNNNNNNGG 4 cut(s) 639, 1212, 1461, 1597
AgsI TTSAA 5 cut(s) 431, 1018, 1045, 1266, 1273
AjnI CCWGG 2 cut(s) 498, 1118
Alw21I GWGCWC 4 cut(s) 192, 1035, 1448, 1551
Alw26I GTCTC 1 cut(s) 1128
AlwI GGATC 4 cut(s) 21, 169, 1174, 1561
AoxI GGCC 4 cut(s) 243, 273, 501, 1049
ApeKI GCWGC 5 cut(s) 161, 282, 667, 1504, 1568
ApoI RAATTY 2 cut(s) 287, 806
Asp700I GAANNNNTTC 3 cut(s) 435, 807, 1097
AspS9I GGNCC 1 cut(s) 1465
AsuHPI GGTGA 4 cut(s) 410, 614, 1108, 1371
AvaII GGWCC 1 cut(s) 1465
AxyI CCTNAGG 1 cut(s) 393
BalI TGGCCA 2 cut(s) 245, 503
BanII GRGCYC 3 cut(s) 192, 1035, 1551
Bbv12I GWGCWC 4 cut(s) 192, 1035, 1448, 1551
BbvI GCAGC 5 cut(s) 148, 294, 654, 1516, 1555
BccI CCATC 5 cut(s) 214, 642, 1099, 1311, 1390
BceAI ACGGC 1 cut(s) 1070
BciT130I CCWGG 2 cut(s) 500, 1120
BciVI GTATCC 1 cut(s) 1191
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 1128
BfaI CTAG 8 cut(s) 141, 338, 354, 588, 869, 1338, 1356, 1404
BfmI CTRYAG 3 cut(s) 60, 1281, 1459
BfuI GTATCC 1 cut(s) 1191
BisI GCNGC 6 cut(s) 162, 283, 668, 682, 1505, 1569
BlsI GCNGC 6 cut(s) 163, 284, 669, 683, 1506, 1570
Bme1390I CCNGG 2 cut(s) 500, 1120
Bme18I GGWCC 1 cut(s) 1465
BmgT120I GGNCC 1 cut(s) 1465
BmiI GGNNCC 2 cut(s) 644, 1466
BmrFI CCNGG 2 cut(s) 500, 1120
BmrI ACTGGG 1 cut(s) 1201
BmsI GCATC 1 cut(s) 958
BmuI ACTGGG 1 cut(s) 1201
BpmI CTGGAG 3 cut(s) 544, 712, 1480
BpuEI CTTGAG 1 cut(s) 1411
BsaI GGTCTC 1 cut(s) 1128
BsaJI CCNNGG 5 cut(s) 30, 75, 552, 638, 1052
Bsc4I CCNNNNNNNGG 4 cut(s) 639, 1212, 1461, 1597
Bse1I ACTGG 4 cut(s) 233, 685, 1207, 1497
Bse21I CCTNAGG 1 cut(s) 393
BseBI CCWGG 2 cut(s) 500, 1120
BseDI CCNNGG 5 cut(s) 30, 75, 552, 638, 1052
BseGI GGATG 6 cut(s) 313, 634, 1033, 1141, 1322, 1633
BseLI CCNNNNNNNGG 4 cut(s) 639, 1212, 1461, 1597
BseMII CTCAG 3 cut(s) 384, 1387, 1536
BseNI ACTGG 4 cut(s) 233, 685, 1207, 1497
BseRI GAGGAG 2 cut(s) 416, 1361
BseXI GCAGC 5 cut(s) 148, 294, 654, 1516, 1555
BsgI GTGCAG 1 cut(s) 1523
BshFI GGCC 4 cut(s) 245, 275, 503, 1051
BsiHKAI GWGCWC 4 cut(s) 192, 1035, 1448, 1551
BslFI GGGAC 2 cut(s) 616, 1436
BslI CCNNNNNNNGG 4 cut(s) 639, 1212, 1461, 1597
BsmAI GTCTC 1 cut(s) 1128
BsmFI GGGAC 2 cut(s) 616, 1436
BsmI GAATGC 2 cut(s) 1097, 1099
BsnI GGCC 4 cut(s) 245, 275, 503, 1051
Bso31I GGTCTC 1 cut(s) 1128
Bsp1286I GDGCHC 4 cut(s) 192, 1035, 1448, 1551
Bsp143I GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
Bsp19I CCATGG 2 cut(s) 75, 638
BspACI CCGC 2 cut(s) 682, 1183
BspANI GGCC 4 cut(s) 245, 275, 503, 1051
BspCNI CTCAG 3 cut(s) 385, 1386, 1537
BspLI GGNNCC 2 cut(s) 644, 1466
BspMAI CTGCAG 1 cut(s) 1463
BspPI GGATC 4 cut(s) 21, 169, 1174, 1561
BspTNI GGTCTC 1 cut(s) 1128
BsrI ACTGG 4 cut(s) 233, 685, 1207, 1497
BssECI CCNNGG 5 cut(s) 30, 75, 552, 638, 1052
BssMI GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
BssNAI GTATAC 1 cut(s) 833
BssT1I CCWWGG 4 cut(s) 30, 75, 552, 638
Bst1107I GTATAC 1 cut(s) 833
Bst2UI CCWGG 2 cut(s) 500, 1120
Bst4CI ACNGT 6 cut(s) 55, 64, 482, 1363, 1450, 1637
BstC8I GCNNGC 2 cut(s) 647, 1566
BstDEI CTNAG 5 cut(s) 393, 568, 1022, 1373, 1545
BstDSI CCRYGG 3 cut(s) 75, 638, 1052
BstEII GGTNACC 1 cut(s) 575
BstENI CCTNNNNNAGG 2 cut(s) 1459, 1595
BstF5I GGATG 6 cut(s) 313, 634, 1033, 1141, 1322, 1633
BstKTI GATC 8 cut(s) 29, 69, 96, 177, 927, 1182, 1307, 1556
BstMAI GTCTC 1 cut(s) 1128
BstMBI GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
BstMWI GCNNNNNNNGC 2 cut(s) 202, 1555
BstNI CCWGG 2 cut(s) 500, 1120
BstPI GGTNACC 1 cut(s) 575
BstSCI CCNGG 2 cut(s) 498, 1118
BstSFI CTRYAG 3 cut(s) 60, 1281, 1459
BstV1I GCAGC 5 cut(s) 148, 294, 654, 1516, 1555
BstX2I RGATCY 1 cut(s) 174
BstXI CCANNNNNNTGG 1 cut(s) 499
BstYI RGATCY 1 cut(s) 174
BstZ17I GTATAC 1 cut(s) 833
Bsu36I CCTNAGG 1 cut(s) 393
BsuI GTATCC 1 cut(s) 1191
BsuRI GGCC 4 cut(s) 245, 275, 503, 1051
BtgI CCRYGG 3 cut(s) 75, 638, 1052
BtsCI GGATG 6 cut(s) 313, 634, 1033, 1141, 1322, 1633
BtsI GCAGTG 1 cut(s) 69
BtsIMutI CAGTG 4 cut(s) 69, 69, 226, 1504
Cac8I GCNNGC 2 cut(s) 647, 1566
Cfr13I GGNCC 1 cut(s) 1465
CsiI ACCWGGT 1 cut(s) 1118
Csp6I GTAC 4 cut(s) 892, 939, 1227, 1359
CviQI GTAC 4 cut(s) 892, 939, 1227, 1359
DdeI CTNAG 5 cut(s) 393, 568, 1022, 1373, 1545
DpnI GATC 8 cut(s) 28, 68, 95, 176, 926, 1181, 1306, 1555
DpnII GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
DraIII CACNNNGTG 1 cut(s) 628
EaeI YGGCCR 2 cut(s) 243, 501
Ecl136II GAGCTC 3 cut(s) 190, 1033, 1549
Eco130I CCWWGG 4 cut(s) 30, 75, 552, 638
Eco24I GRGCYC 3 cut(s) 192, 1035, 1551
Eco31I GGTCTC 1 cut(s) 1128
Eco32I GATATC 2 cut(s) 316, 1083
Eco47I GGWCC 1 cut(s) 1465
Eco53kI GAGCTC 3 cut(s) 190, 1033, 1549
Eco57I CTGAAG 2 cut(s) 262, 1207
Eco81I CCTNAGG 1 cut(s) 393
Eco91I GGTNACC 1 cut(s) 575
EcoICRI GAGCTC 3 cut(s) 190, 1033, 1549
EcoNI CCTNNNNNAGG 2 cut(s) 1459, 1595
EcoO65I GGTNACC 1 cut(s) 575
EcoRII CCWGG 2 cut(s) 498, 1118
EcoRV GATATC 2 cut(s) 316, 1083
EcoT14I CCWWGG 4 cut(s) 30, 75, 552, 638
EcoT22I ATGCAT 1 cut(s) 1099
EcoT38I GRGCYC 3 cut(s) 192, 1035, 1551
ErhI CCWWGG 4 cut(s) 30, 75, 552, 638
FaqI GGGAC 2 cut(s) 616, 1436
FauNDI CATATG 1 cut(s) 854
FbaI TGATCA 1 cut(s) 66
FblI GTMKAC 1 cut(s) 832
Fnu4HI GCNGC 6 cut(s) 162, 283, 668, 682, 1505, 1569
FokI GGATG 6 cut(s) 320, 621, 1040, 1128, 1329, 1640
FriOI GRGCYC 3 cut(s) 192, 1035, 1551
Fsp4HI GCNGC 6 cut(s) 162, 283, 668, 682, 1505, 1569
FspBI CTAG 8 cut(s) 141, 338, 354, 588, 869, 1338, 1356, 1404
GluI GCNGC 6 cut(s) 162, 283, 668, 682, 1505, 1569
GsuI CTGGAG 3 cut(s) 544, 712, 1480
HaeIII GGCC 4 cut(s) 245, 275, 503, 1051
HincII GTYRAC 1 cut(s) 609
HindII GTYRAC 1 cut(s) 609
HindIII AAGCTT 1 cut(s) 752
HinfI GANTC 7 cut(s) 325, 404, 466, 735, 1368, 1400, 1541
HphI GGTGA 4 cut(s) 410, 614, 1108, 1371
Hpy166II GTNNAC 6 cut(s) 478, 609, 622, 833, 1116, 1379
Hpy188I TCNGA 7 cut(s) 564, 599, 954, 1291, 1399, 1546, 1553
Hpy8I GTNNAC 6 cut(s) 478, 609, 622, 833, 1116, 1379
HpyCH4III ACNGT 6 cut(s) 55, 64, 482, 1363, 1450, 1637
HpyCH4IV ACGT 1 cut(s) 1112
HpyCH4V TGCA 5 cut(s) 861, 949, 1097, 1461, 1504
HpyF10VI GCNNNNNNNGC 2 cut(s) 202, 1555
HpyF3I CTNAG 5 cut(s) 393, 568, 1022, 1373, 1545
HpySE526I ACGT 1 cut(s) 1112
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
LmnI GCTCC 3 cut(s) 642, 792, 1443
Lsp1109I GCAGC 5 cut(s) 148, 294, 654, 1516, 1555
LweI GCATC 1 cut(s) 958
MabI ACCWGGT 1 cut(s) 1118
MaeI CTAG 8 cut(s) 141, 338, 354, 588, 869, 1338, 1356, 1404
MaeII ACGT 1 cut(s) 1112
MaeIII GTNAC 6 cut(s) 224, 575, 786, 884, 1490, 1580
MalI GATC 8 cut(s) 28, 68, 95, 176, 926, 1181, 1306, 1555
MboI GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
MflI RGATCY 1 cut(s) 174
MhlI GDGCHC 4 cut(s) 192, 1035, 1448, 1551
MlsI TGGCCA 2 cut(s) 245, 503
MluCI AATT 6 cut(s) 84, 287, 778, 806, 965, 1134
MluNI TGGCCA 2 cut(s) 245, 503
MlyI GAGTC 5 cut(s) 334, 413, 460, 1362, 1550
MmeI TCCRAC 2 cut(s) 111, 1345
Mox20I TGGCCA 2 cut(s) 245, 503
Mph1103I ATGCAT 1 cut(s) 1099
MroXI GAANNNNTTC 3 cut(s) 435, 807, 1097
MscI TGGCCA 2 cut(s) 245, 503
MseI TTAA 5 cut(s) 83, 258, 446, 510, 1137
MslI CAYNNNNRTG 4 cut(s) 46, 74, 1256, 1626
Msp20I TGGCCA 2 cut(s) 245, 503
MspR9I CCNGG 2 cut(s) 500, 1120
Mva1269I GAATGC 2 cut(s) 1097, 1099
MvaI CCWGG 2 cut(s) 500, 1120
MwoI GCNNNNNNNGC 2 cut(s) 202, 1555
NcoI CCATGG 2 cut(s) 75, 638
NdeI CATATG 1 cut(s) 854
NdeII GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
NlaIV GGNNCC 2 cut(s) 644, 1466
NmuCI GTSAC 2 cut(s) 224, 1580
NsiI ATGCAT 1 cut(s) 1099
PctI GAATGC 2 cut(s) 1097, 1099
PdmI GAANNNNTTC 3 cut(s) 435, 807, 1097
PfeI GAWTC 2 cut(s) 735, 1400
PflFI GACNNNGTC 1 cut(s) 1344
PkrI GCNGC 6 cut(s) 163, 284, 669, 683, 1506, 1570
PleI GAGTC 5 cut(s) 333, 412, 460, 1362, 1549
PpsI GAGTC 5 cut(s) 333, 412, 460, 1362, 1549
Psp124BI GAGCTC 3 cut(s) 192, 1035, 1551
Psp6I CCWGG 2 cut(s) 498, 1118
PspEI GGTNACC 1 cut(s) 575
PspGI CCWGG 2 cut(s) 498, 1118
PspN4I GGNNCC 2 cut(s) 644, 1466
PspPI GGNCC 1 cut(s) 1465
PstI CTGCAG 1 cut(s) 1463
PsuI RGATCY 1 cut(s) 174
PsyI GACNNNGTC 1 cut(s) 1344
RsaI GTAC 4 cut(s) 893, 940, 1228, 1360
RsaNI GTAC 4 cut(s) 892, 939, 1227, 1359
RseI CAYNNNNRTG 4 cut(s) 46, 74, 1256, 1626
SacI GAGCTC 3 cut(s) 192, 1035, 1551
SaqAI TTAA 5 cut(s) 83, 258, 446, 510, 1137
SatI GCNGC 6 cut(s) 162, 283, 668, 682, 1505, 1569
Sau3AI GATC 8 cut(s) 26, 66, 93, 174, 924, 1179, 1304, 1553
Sau96I GGNCC 1 cut(s) 1465
SbfI CCTGCAGG 1 cut(s) 1463
SchI GAGTC 5 cut(s) 334, 413, 460, 1362, 1550
ScrFI CCNGG 2 cut(s) 500, 1120
SdaI CCTGCAGG 1 cut(s) 1463
SduI GDGCHC 4 cut(s) 192, 1035, 1448, 1551
SexAI ACCWGGT 1 cut(s) 1118
SfaNI GCATC 1 cut(s) 958
SfcI CTRYAG 3 cut(s) 60, 1281, 1459
SinI GGWCC 1 cut(s) 1465
SmiMI CAYNNNNRTG 4 cut(s) 46, 74, 1256, 1626
SmlI CTYRAG 1 cut(s) 1426
SmoI CTYRAG 1 cut(s) 1426
Sse8387I CCTGCAGG 1 cut(s) 1463
Sse9I AATT 6 cut(s) 84, 287, 778, 806, 965, 1134
SsiI CCGC 2 cut(s) 682, 1183
SspI AATATT 2 cut(s) 386, 450
SspMI CTAG 8 cut(s) 141, 338, 354, 588, 869, 1338, 1356, 1404
SstI GAGCTC 3 cut(s) 192, 1035, 1551
StyD4I CCNGG 2 cut(s) 498, 1118
StyI CCWWGG 4 cut(s) 30, 75, 552, 638
TaaI ACNGT 6 cut(s) 55, 64, 482, 1363, 1450, 1637
TaiI ACGT 1 cut(s) 1115
TaqI TCGA 3 cut(s) 25, 323, 1101
TasI AATT 6 cut(s) 84, 287, 778, 806, 965, 1134
TatI WGTACW 2 cut(s) 891, 1226
TauI GCSGC 1 cut(s) 684
TfiI GAWTC 2 cut(s) 735, 1400
Tru1I TTAA 5 cut(s) 83, 258, 446, 510, 1137
Tru9I TTAA 5 cut(s) 83, 258, 446, 510, 1137
TscAI CASTG 4 cut(s) 69, 76, 233, 1504
TseFI GTSAC 2 cut(s) 224, 1580
TseI GCWGC 5 cut(s) 161, 282, 667, 1504, 1568
Tsp45I GTSAC 2 cut(s) 224, 1580
TspGWI ACGGA 2 cut(s) 805, 1081
TspRI CASTG 4 cut(s) 69, 76, 233, 1504
Tth111I GACNNNGTC 1 cut(s) 1344
VpaK11BI GGWCC 1 cut(s) 1465
XagI CCTNNNNNAGG 2 cut(s) 1459, 1595
XapI RAATTY 2 cut(s) 287, 806
XbaI TCTAGA 2 cut(s) 337, 353
XcmI CCANNNNNNNNNTGG 1 cut(s) 52
XmiI GTMKAC 1 cut(s) 832
XmnI GAANNNNTTC 3 cut(s) 435, 807, 1097
XspI CTAG 8 cut(s) 141, 338, 354, 588, 869, 1338, 1356, 1404
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.