Rh2DG131700

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0006904
Physical Location & Seq
Reverse (-)
11112213 .. 11117201
4989 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG131700.1

Sequence Viewer

Length: 1653 bp
ATGGAGGAGAGGGAAGGTCGATCCTTGGCTGAAACACCTACATGGACTGTGGCTACTGTGATCACTGTCTTGATTTCTCTTGGGTTCTTCTTTCATTGCTCTTTGAAACATTTTGGAAAGTGGTTGGACAAGACTAAGAAGAAGTCTATACTTGCTGCTTTGGAAAAGATCCAAGAAGAGCTGATGCTTTTTGGGCTTCTGTCATTGTTGATGGGTCACTGGATTGTTTTTGTGGCCAAGATTTGTGTTAAATCTTCATCATGGGCCTTCAGCAGCCGGTTCTTTCCTTGTGCTTTGGAGCAAGAGATCAGTGTGATTCAACATGCACTTTTTGCGAGTCCGGAGTATCTGAACAAGTCAGTTTCTAGAGTGGAAGTGAAAACTATGTCTCATGAATATTGTCCTAAGGGTCATGAGTCCTTTGCTTCTTATGAAAGTCTTGAACAGCTTCACCGATTAATATTCGTGCTTGGCATGACTCACGTTGTTTACAGTTTCTTTGCCATTGTCATGGCCATGATTAAGATTTATAGCTGGAGAACATGGGAAAATGAAGCTAAAGCTAAGGCAATTCAAACCTTTAAAGATACCGAAGAAACTAAGCGAATCAGAAGATTGTCGACATTCATAGTTCACCATGCATCTCATCCATGGAGCCAGCACAAGTATCTTGTTTGGCTGCTTTGTTTCAGCCGCCAGTTCTGGAGTTCTATAAACCGACCTTCATATATGGCTTTGCGATTTGGATTCATCACTACTCATCAGCTTCCTTTGTCATATGATTTCCATAATTATATGATCCGGAGCATGGAGGAAGAATTTCGTGATATTGTTGGTATCAGTATACCTCTCTGGATATACGCCTTATGTTGCGTTATTCTAGATTTTCATGGAAGCAACATTTACTTTTGGCTTTCCTTCCTTCCAGCGATTTTGATCCTGCTTGTCGGCACTAAACTGCATCGGATAGTGGTAAAATTGGCTGTGGAAATCATGGAAGAAAGTCCATATATGGAAAATCACCAGTTTAAACTAAGGGATGATCTCTTTTGGTTCAAGAGGCCCTGGCTTCTTTTACGGCTAATACAACTGATATCCTTCCAGAATTCATTCGAGATGGCTACTTTCCTCTGGTCTCTGTGGGAAATTAAACATCCTTCATGTTTCATGGACAATCATACTTTCCTCGTGATCCGCTTGACATTTGGGGTAATCTCCCAGTTCTGGTGTAGCTTCATTACATTCCCACTCTATGTTATCATCACACAAATGGGTTCAAGCTTCAAGAAATCTGTAGTTTCCGAAAATGTAAGACAATTGCTATCTGGATGGAAAAGGAGAGTGAAGGCGAGACAAAGTACATCTAATAAGACTGGACTCCTGAATTCATCATCAACATCAACGGTAGCATTCTCATCAGATTCTAGCAAAGTTTGTAGTTCTTTTTCCTCGAACAGCGTGGAAGGGAGCTCAGTCTCCTCCAGGTTCCAGAACACATCCTCCCGACATCTAAAGTCCAATGTGCAGCTAGGTGAAGTTAGTGAGATCGATGAGAAGTCAGAGTCTGAGCTCTGCACGCAAACACTCTCTGCTTTGATGAAAGATTATGACGTTTATGAAGAATATGAGGAAGATGAAGAAGAGGCTAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

550

Amino Acids

64.02

Weight (kDa)

8.18

Isoelectric Point (pI)

48.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 6 - 478 1e-158 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 620, 844
AccIII TCCGGA 2 cut(s) 340, 801
AciI CCGC 2 cut(s) 694, 1195
AclWI GGATC 5 cut(s) 15, 163, 793, 931, 1186
AcoI YGGCCR 2 cut(s) 234, 513
AcsI RAATTY 3 cut(s) 818, 1105, 1384
AcuI CTGAAG 1 cut(s) 253
AfaI GTAC 1 cut(s) 1360
AfiI CCNNNNNNNGG 2 cut(s) 808, 1224
AgsI TTSAA 7 cut(s) 106, 320, 443, 575, 1057, 1278, 1285
AjnI CCWGG 2 cut(s) 1064, 1481
AloI GAACNNNNNNTCC 2 cut(s) 1484, 1516
Alw21I GWGCWC 2 cut(s) 1472, 1572
Alw26I GTCTC 4 cut(s) 393, 1140, 1345, 1480
AlwI GGATC 5 cut(s) 15, 163, 793, 931, 1186
AlwNI CAGNNNCTG 1 cut(s) 1565
Aor13HI TCCGGA 2 cut(s) 340, 801
AoxI GGCC 4 cut(s) 234, 264, 513, 1061
ApeKI GCWGC 4 cut(s) 155, 273, 679, 1525
ApoI RAATTY 3 cut(s) 818, 1105, 1384
AseI ATTAAT 1 cut(s) 458
Asp700I GAANNNNTTC 3 cut(s) 447, 819, 1109
AspS9I GGNCC 2 cut(s) 264, 1062
AsuHPI GGTGA 4 cut(s) 443, 626, 1013, 1544
AxyI CCTNAGG 1 cut(s) 405
BalI TGGCCA 2 cut(s) 236, 515
BanII GRGCYC 2 cut(s) 1472, 1572
BauI CACGAG 1 cut(s) 1187
Bbv12I GWGCWC 2 cut(s) 1472, 1572
BbvI GCAGC 4 cut(s) 142, 285, 666, 1537
BccI CCATC 3 cut(s) 205, 1111, 1323
BceAI ACGGC 1 cut(s) 1094
BciT130I CCWGG 2 cut(s) 1066, 1483
BclI TGATCA 1 cut(s) 60
BcoDI GTCTC 4 cut(s) 393, 1140, 1345, 1480
BfaI CTAG 4 cut(s) 366, 881, 1425, 1529
BfmI CTRYAG 1 cut(s) 1293
BisI GCNGC 5 cut(s) 156, 274, 680, 694, 1526
BlsI GCNGC 5 cut(s) 157, 275, 681, 695, 1527
Bme1390I CCNGG 2 cut(s) 1066, 1483
BmgT120I GGNCC 2 cut(s) 264, 1062
BmiI GGNNCC 2 cut(s) 656, 1487
BmrFI CCNGG 2 cut(s) 1066, 1483
BmrI ACTGGG 1 cut(s) 1213
BmsI GCATC 3 cut(s) 174, 650, 970
BmuI ACTGGG 1 cut(s) 1213
BpmI CTGGAG 3 cut(s) 556, 724, 1465
Bpu10I CCTNAGC 1 cut(s) 564
Bsa29I ATCGAT 1 cut(s) 1548
BsaBI GATNNNNATC 1 cut(s) 935
BsaI GGTCTC 1 cut(s) 1140
BsaJI CCNNGG 3 cut(s) 24, 650, 1064
BsaWI WCCGGW 2 cut(s) 340, 801
BsaXI ACNNNNNCTCC 2 cut(s) 1484, 1514
Bsc4I CCNNNNNNNGG 2 cut(s) 808, 1224
Bse118I RCCGGY 1 cut(s) 276
Bse1I ACTGG 5 cut(s) 224, 697, 1024, 1219, 1378
Bse21I CCTNAGG 1 cut(s) 405
Bse3DI GCAATG 1 cut(s) 94
Bse8I GATNNNNATC 1 cut(s) 935
BseAI TCCGGA 2 cut(s) 340, 801
BseBI CCWGG 2 cut(s) 1066, 1483
BseCI ATCGAT 1 cut(s) 1548
BseDI CCNNGG 3 cut(s) 24, 650, 1064
BseGI GGATG 5 cut(s) 646, 1045, 1153, 1334, 1496
BseJI GATNNNNATC 1 cut(s) 935
BseLI CCNNNNNNNGG 2 cut(s) 808, 1224
BseMI GCAATG 1 cut(s) 94
BseMII CTCAG 2 cut(s) 1485, 1557
BseNI ACTGG 5 cut(s) 224, 697, 1024, 1219, 1378
BseRI GAGGAG 2 cut(s) 20, 1468
BseXI GCAGC 4 cut(s) 142, 285, 666, 1537
BsgI GTGCAG 2 cut(s) 1544, 1558
BshFI GGCC 4 cut(s) 236, 266, 515, 1063
BshVI ATCGAT 1 cut(s) 1548
BsiHKAI GWGCWC 2 cut(s) 1472, 1572
BsiSI CCGG 3 cut(s) 277, 341, 802
BslI CCNNNNNNNGG 2 cut(s) 808, 1224
BsmAI GTCTC 4 cut(s) 393, 1140, 1345, 1480
BsmI GAATGC 1 cut(s) 1409
BsnI GGCC 4 cut(s) 236, 266, 515, 1063
Bso31I GGTCTC 1 cut(s) 1140
Bsp1286I GDGCHC 2 cut(s) 1472, 1572
Bsp13I TCCGGA 2 cut(s) 340, 801
Bsp143I GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
Bsp19I CCATGG 1 cut(s) 650
BspACI CCGC 2 cut(s) 694, 1195
BspANI GGCC 4 cut(s) 236, 266, 515, 1063
BspCNI CTCAG 2 cut(s) 1484, 1558
BspDI ATCGAT 1 cut(s) 1548
BspEI TCCGGA 2 cut(s) 340, 801
BspHI TCATGA 2 cut(s) 391, 412
BspLI GGNNCC 2 cut(s) 656, 1487
BspPI GGATC 5 cut(s) 15, 163, 793, 931, 1186
BspQI GCTCTTC 1 cut(s) 171
BspTNI GGTCTC 1 cut(s) 1140
BsrDI GCAATG 1 cut(s) 94
BsrFI RCCGGY 1 cut(s) 276
BsrI ACTGG 5 cut(s) 224, 697, 1024, 1219, 1378
BssAI RCCGGY 1 cut(s) 276
BssECI CCNNGG 3 cut(s) 24, 650, 1064
BssMI GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
BssNAI GTATAC 1 cut(s) 845
BssSI CACGAG 1 cut(s) 1187
BssT1I CCWWGG 2 cut(s) 24, 650
Bst1107I GTATAC 1 cut(s) 845
Bst2BI CACGAG 1 cut(s) 1187
Bst2UI CCWGG 2 cut(s) 1066, 1483
Bst4CI ACNGT 5 cut(s) 49, 58, 67, 494, 1405
Bst6I CTCTTC 2 cut(s) 171, 1635
BstAPI GCANNNNNTGC 1 cut(s) 332
BstC8I GCNNGC 2 cut(s) 659, 1577
BstDEI CTNAG 7 cut(s) 135, 405, 564, 600, 1034, 1471, 1566
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 5 cut(s) 646, 1045, 1153, 1334, 1496
BstKTI GATC 9 cut(s) 23, 63, 171, 309, 801, 939, 1045, 1194, 1548
BstMAI GTCTC 4 cut(s) 393, 1140, 1345, 1480
BstMBI GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
BstMWI GCNNNNNNNGC 3 cut(s) 193, 332, 1576
BstNI CCWGG 2 cut(s) 1066, 1483
BstNSI RCATGY 1 cut(s) 326
BstSCI CCNGG 2 cut(s) 1064, 1481
BstSFI CTRYAG 1 cut(s) 1293
BstV1I GCAGC 4 cut(s) 142, 285, 666, 1537
BstX2I RGATCY 1 cut(s) 168
BstXI CCANNNNNNTGG 1 cut(s) 511
BstYI RGATCY 1 cut(s) 168
BstZ17I GTATAC 1 cut(s) 845
Bsu15I ATCGAT 1 cut(s) 1548
Bsu36I CCTNAGG 1 cut(s) 405
BsuRI GGCC 4 cut(s) 236, 266, 515, 1063
BsuTUI ATCGAT 1 cut(s) 1548
BtgI CCRYGG 1 cut(s) 650
BtsCI GGATG 5 cut(s) 646, 1045, 1153, 1334, 1496
BtsIMutI CAGTG 3 cut(s) 63, 217, 316
Cac8I GCNNGC 2 cut(s) 659, 1577
CaiI CAGNNNCTG 1 cut(s) 1565
CciI TCATGA 2 cut(s) 391, 412
Cfr10I RCCGGY 1 cut(s) 276
Cfr13I GGNCC 2 cut(s) 264, 1062
ClaI ATCGAT 1 cut(s) 1548
Csp6I GTAC 1 cut(s) 1359
CviQI GTAC 1 cut(s) 1359
DdeI CTNAG 7 cut(s) 135, 405, 564, 600, 1034, 1471, 1566
DpnI GATC 9 cut(s) 22, 62, 170, 308, 800, 938, 1044, 1193, 1547
DpnII GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
DraI TTTAAA 2 cut(s) 583, 1030
EaeI YGGCCR 2 cut(s) 234, 513
Eam1104I CTCTTC 2 cut(s) 171, 1635
EarI CTCTTC 2 cut(s) 171, 1635
Ecl136II GAGCTC 2 cut(s) 1470, 1570
Eco130I CCWWGG 2 cut(s) 24, 650
Eco24I GRGCYC 2 cut(s) 1472, 1572
Eco31I GGTCTC 1 cut(s) 1140
Eco32I GATATC 1 cut(s) 1095
Eco53kI GAGCTC 2 cut(s) 1470, 1570
Eco57I CTGAAG 1 cut(s) 253
Eco81I CCTNAGG 1 cut(s) 405
EcoICRI GAGCTC 2 cut(s) 1470, 1570
EcoO109I RGGNCCY 1 cut(s) 1062
EcoRI GAATTC 2 cut(s) 1105, 1384
EcoRII CCWGG 2 cut(s) 1064, 1481
EcoRV GATATC 1 cut(s) 1095
EcoT14I CCWWGG 2 cut(s) 24, 650
EcoT22I ATGCAT 1 cut(s) 643
EcoT38I GRGCYC 2 cut(s) 1472, 1572
ErhI CCWWGG 2 cut(s) 24, 650
FauNDI CATATG 1 cut(s) 778
FbaI TGATCA 1 cut(s) 60
FblI GTMKAC 2 cut(s) 620, 844
Fnu4HI GCNGC 5 cut(s) 156, 274, 680, 694, 1526
FokI GGATG 5 cut(s) 633, 1052, 1140, 1341, 1483
FriOI GRGCYC 2 cut(s) 1472, 1572
Fsp4HI GCNGC 5 cut(s) 156, 274, 680, 694, 1526
FspBI CTAG 4 cut(s) 366, 881, 1425, 1529
GluI GCNGC 5 cut(s) 156, 274, 680, 694, 1526
GsuI CTGGAG 3 cut(s) 556, 724, 1465
HaeIII GGCC 4 cut(s) 236, 266, 515, 1063
HapII CCGG 3 cut(s) 277, 341, 802
HincII GTYRAC 1 cut(s) 621
HindII GTYRAC 1 cut(s) 621
HindIII AAGCTT 1 cut(s) 1279
HinfI GANTC 9 cut(s) 316, 337, 416, 478, 606, 747, 1377, 1421, 1562
HpaII CCGG 3 cut(s) 277, 341, 802
HphI GGTGA 4 cut(s) 443, 626, 1013, 1544
Hpy166II GTNNAC 4 cut(s) 490, 621, 634, 845
Hpy188I TCNGA 7 cut(s) 351, 611, 966, 1303, 1420, 1561, 1567
Hpy8I GTNNAC 4 cut(s) 490, 621, 634, 845
HpyAV CCTTC 9 cut(s) 8, 277, 732, 928, 932, 1108, 1167, 1339, 1457
HpyCH4III ACNGT 5 cut(s) 49, 58, 67, 494, 1405
HpyCH4IV ACGT 2 cut(s) 483, 1611
HpyCH4V TGCA 5 cut(s) 326, 641, 961, 1525, 1575
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 332, 1576
HpyF3I CTNAG 7 cut(s) 135, 405, 564, 600, 1034, 1471, 1566
HpySE526I ACGT 2 cut(s) 483, 1611
Kpn2I TCCGGA 2 cut(s) 340, 801
Ksp22I TGATCA 1 cut(s) 60
Kzo9I GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 4 cut(s) 298, 654, 804, 1467
Lsp1109I GCAGC 4 cut(s) 142, 285, 666, 1537
LweI GCATC 3 cut(s) 174, 650, 970
MaeI CTAG 4 cut(s) 366, 881, 1425, 1529
MaeII ACGT 2 cut(s) 483, 1611
MaeIII GTNAC 1 cut(s) 215
MalI GATC 9 cut(s) 22, 62, 170, 308, 800, 938, 1044, 1193, 1547
MboI GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
MfeI CAATTG 1 cut(s) 1316
MflI RGATCY 1 cut(s) 168
MhlI GDGCHC 2 cut(s) 1472, 1572
MlsI TGGCCA 2 cut(s) 236, 515
MluCI AATT 8 cut(s) 570, 790, 818, 977, 1105, 1146, 1316, 1384
MluNI TGGCCA 2 cut(s) 236, 515
MlyI GAGTC 5 cut(s) 346, 425, 472, 1371, 1571
MmeI TCCRAC 1 cut(s) 105
Mox20I TGGCCA 2 cut(s) 236, 515
Mph1103I ATGCAT 1 cut(s) 643
MroI TCCGGA 2 cut(s) 340, 801
MroXI GAANNNNTTC 3 cut(s) 447, 819, 1109
MscI TGGCCA 2 cut(s) 236, 515
MseI TTAA 6 cut(s) 249, 458, 522, 582, 1029, 1149
MslI CAYNNNNRTG 4 cut(s) 40, 509, 515, 1268
Msp20I TGGCCA 2 cut(s) 236, 515
MspI CCGG 3 cut(s) 277, 341, 802
MspR9I CCNGG 2 cut(s) 1066, 1483
MssI GTTTAAAC 1 cut(s) 1030
MunI CAATTG 1 cut(s) 1316
Mva1269I GAATGC 1 cut(s) 1409
MvaI CCWGG 2 cut(s) 1066, 1483
MwoI GCNNNNNNNGC 3 cut(s) 193, 332, 1576
NcoI CCATGG 1 cut(s) 650
NdeI CATATG 1 cut(s) 778
NdeII GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
NlaIV GGNNCC 2 cut(s) 656, 1487
NmuCI GTSAC 1 cut(s) 215
NsiI ATGCAT 1 cut(s) 643
NspI RCATGY 1 cut(s) 326
PagI TCATGA 2 cut(s) 391, 412
PciSI GCTCTTC 1 cut(s) 171
PctI GAATGC 1 cut(s) 1409
PdmI GAANNNNTTC 3 cut(s) 447, 819, 1109
PfeI GAWTC 4 cut(s) 316, 606, 747, 1421
PkrI GCNGC 5 cut(s) 157, 275, 681, 695, 1527
PleI GAGTC 5 cut(s) 345, 424, 472, 1371, 1570
PmeI GTTTAAAC 1 cut(s) 1030
PpsI GAGTC 5 cut(s) 345, 424, 472, 1371, 1570
PshBI ATTAAT 1 cut(s) 458
Psp124BI GAGCTC 2 cut(s) 1472, 1572
Psp6I CCWGG 2 cut(s) 1064, 1481
PspGI CCWGG 2 cut(s) 1064, 1481
PspN4I GGNNCC 2 cut(s) 656, 1487
PspPI GGNCC 2 cut(s) 264, 1062
PstNI CAGNNNCTG 1 cut(s) 1565
PsuI RGATCY 1 cut(s) 168
RsaI GTAC 1 cut(s) 1360
RsaNI GTAC 1 cut(s) 1359
RseI CAYNNNNRTG 4 cut(s) 40, 509, 515, 1268
SacI GAGCTC 2 cut(s) 1472, 1572
SalI GTCGAC 1 cut(s) 619
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 6 cut(s) 249, 458, 522, 582, 1029, 1149
SatI GCNGC 5 cut(s) 156, 274, 680, 694, 1526
Sau3AI GATC 9 cut(s) 20, 60, 168, 306, 798, 936, 1042, 1191, 1545
Sau96I GGNCC 2 cut(s) 264, 1062
SchI GAGTC 5 cut(s) 346, 425, 472, 1371, 1571
ScrFI CCNGG 2 cut(s) 1066, 1483
SduI GDGCHC 2 cut(s) 1472, 1572
SfaNI GCATC 3 cut(s) 174, 650, 970
SfcI CTRYAG 1 cut(s) 1293
SmiMI CAYNNNNRTG 4 cut(s) 40, 509, 515, 1268
Sse9I AATT 8 cut(s) 570, 790, 818, 977, 1105, 1146, 1316, 1384
SsiI CCGC 2 cut(s) 694, 1195
SspI AATATT 2 cut(s) 398, 462
SspMI CTAG 4 cut(s) 366, 881, 1425, 1529
SstI GAGCTC 2 cut(s) 1472, 1572
StyD4I CCNGG 2 cut(s) 1064, 1481
StyI CCWWGG 2 cut(s) 24, 650
TaaI ACNGT 5 cut(s) 49, 58, 67, 494, 1405
TaiI ACGT 2 cut(s) 486, 1614
TaqI TCGA 5 cut(s) 19, 620, 1113, 1451, 1548
TasI AATT 8 cut(s) 570, 790, 818, 977, 1105, 1146, 1316, 1384
TatI WGTACW 1 cut(s) 1358
TauI GCSGC 1 cut(s) 696
TfiI GAWTC 4 cut(s) 316, 606, 747, 1421
Tru1I TTAA 6 cut(s) 249, 458, 522, 582, 1029, 1149
Tru9I TTAA 6 cut(s) 249, 458, 522, 582, 1029, 1149
TscAI CASTG 3 cut(s) 70, 224, 316
TseFI GTSAC 1 cut(s) 215
TseI GCWGC 4 cut(s) 155, 273, 679, 1525
Tsp45I GTSAC 1 cut(s) 215
TspRI CASTG 3 cut(s) 70, 224, 316
VspI ATTAAT 1 cut(s) 458
XapI RAATTY 3 cut(s) 818, 1105, 1384
XbaI TCTAGA 2 cut(s) 365, 880
XceI RCATGY 1 cut(s) 326
XmiI GTMKAC 2 cut(s) 620, 844
XmnI GAANNNNTTC 3 cut(s) 447, 819, 1109
XspI CTAG 4 cut(s) 366, 881, 1425, 1529
Zsp2I ATGCAT 1 cut(s) 643
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.